An evaluation of a particle beam interface at high aqueous ...
Transcript of An evaluation of a particle beam interface at high aqueous ...
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An Evaluation of a ParticleBeam Interface at High
Aqueous Concentrations
Jennifer M. Duker and Thomas C. Marecic
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Problem...
Reverse phase HPLC can typically include highaqueous solvent conditions.
MS is becoming a preferred detection device forHPLC.
Compatibility between the two techniques ischallenging.
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Stages of Particle Beam LC/MS
HPLC INTERFACE MASSDETECTOR
Phase Momentum
LC Separate Separate MSLiquid _ "- "- _ GasPhase Phase
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INTERFAC
Phase Momentum
Separate Separate
Aerosol Enrichment/generation Pressure
ReductionDesolvation
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Phase Separation
GoalTo separate the solvent from thesolute by converting the solvent tothe gas phase and the solute t°particles
° Nebulization - generation of anaerosol
° Desolvation - separation of thesolvent from the solute
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Momentum Separation
,-GoalTo remove gas phase whiletransporting particles into MS
° Effective removal of solvent• Enrichment of solute
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INTERFACE
Phase Momentum
Separate Separate
Aerosol Enrichment/generation Pressure
ReductionDesolvation
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Why are some solvent conditionsmore difficult?
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Solvent B.Pt.
ProticWater 100 10.5Methanol 64.7 8.9
AproticAcetonitrile 81.6 7.8THF 66.0 7.6
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Phase Separation(Cpdt)g+(Cpdt)soI
(Cpdt)l AHvap
TOcmeasured iDEAL
Power (watts)
100% Solvent Evaporation0% Solute Evaporation
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Summary of Phase SeparationTM
ThermaBeam
Thermal/Pneumatic NebulizationDesolvation
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Analysis of Environmental CompoundsExperimental Conditions
Analytical ConditionsCompounds: caffeine
methomylbenzidine
Column: Waters Nova-Pak C18 (2.0 x 150 mm)Mobile Phases: (1)72:28 ammonium acetate:ACN
(2)87:13 ammonium acetate:ACNFlow rate: 250 uL/min
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MS Review
Ivent Conditions72:28
NH4OAc:ACN
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MaxplotChromatogram
100 to 200 amu
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PDA Review
Fi p
HighAqueousSolvent
Conditions87:13
NH OAc:ACN4
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MS Review
HighAqueousSolvent
Conditions87:13
NH4OAc:ACN
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View Cube
3D
Chromatogram
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TICContour View
MaxplotChromatogram
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Maxplot Chromatogram100 to 200 amu
Environm
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Overlay of Mass Spectra
_ra::_i!_!_ii_i__Hel:::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::
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•Eile',"_!!i_'_i_!_E_ditl;ii__!_PlOt_D,C p
ExtractedMass
Spectrumof methomyl
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__Hetp!!i
Extracta
Mass
Spectrumof
benzidine
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ExtractedMass
Spectrumof benzidine
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Extracta
Mass
Spectrumof
caffeine
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ExtractedMass
Spectrumof caffeine
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Conclusions
Analysis using high aqueoussolvent conditions is possible
,_ThermaBeam TM lessens the
challenge for compatibility betweenHPLC and MS
AutoOptimize adjusts the interfaceto handle high aqueous conditions