An employment of flow cytometry in the biosystematics of the genus Galeobdolon

An employment of flow cytometry in the biosystematics of the genus

Galeobdolon


Galeobdolon

Radka Rosenbaumová

Genus Genus GaleobdolonGaleobdolonGenus Genus GaleobdolonGaleobdolon

x = 9

2x = 184x = 36

3x = 27


G. luteum G. flavidum

2n = 2x = 18


G. montanum

2n = 4x = 36


G. argentatum

2n = 4x = 36


G. montanumG. luteum

2x 4x

G. endtmannii

2n = 2x = 18

Genus Genus GaleobdolonGaleobdolonGenus Genus GaleobdolonGaleobdolonDistribution

-------G. montanum

........

G. flavidum

G. luteum

G. argentatum

G. endtmannii

Ploidy level estimationPloidy level estimationPloidy level estimationPloidy level estimation

Standard: Glycine max cv. Polanka, (2C = 2.5 pg)

0

100

200

300

400

500

0 250 500 750 1000

Standard4x

G. montanumG. argentatum

0

150

300

450

600

750

0 250 500 750 1000

Standard

2x

G. luteumG. flavidum


Triploids

500

400

300

200

100

00 50 100 150 200 250

2x3x

4x


Triploids

Ploidy level estimation Ploidy level estimation Ploidy level estimation Ploidy level estimation

Sympatric occurence

12%

G. luteumG. montanum G. argentatum


G. endtmannii


G. luteum

2x 4x

G. montanum

2x + 4x

4x

G. endtmannii

2x

Nuclear genome size determination Nuclear genome size determination Nuclear genome size determination Nuclear genome size determination

Species 2C DNA content ± SE

(pg)

1C DNA content (pg / Mbp)

Genome size (pg)

G. luteum 3.27 ± 0.002 1.63 / 1597 1.63

„G. endtmannii“ 3.28 ± 0.02 1.64 / 1607 1.64

G. flavidum 3.41 ± 0.02 1.70 / 1666 1.70

G. montanum 6.33 ± 0.04 3.17 / 3107 1.58

G. argentatum 6.36 ± 0.04 3.18 / 3116 1.59

„G. montanum“ 6.59 ± 0.04 3.29 / 3224 1.65

„G. endtmannii“ 6.41 ± 0.03 3.20 / 3136 1.60

Triploids 4.85 ± 0.04 2.43 / 2381 1.62


1.622.43 / 23814.85 ± 0.04Triploids

1.603.20 / 31366.41 ± 0.03„G. endtmannii“

1.653.29 / 32246.59 ± 0.04„G. montanum“

1.593.18 / 31166.36 ± 0.04G. argentatum

1.583.17 / 31076.33 ± 0.04G. montanum

1.701.70 / 16663.41 ± 0.02G. flavidum

1.641.64 / 16073.28 ± 0.02„G. endtmannii“

1.631.63 / 15973.27 ± 0.002G. luteum

Genome size (pg)


2C DNA content ± SE

(pg)

Species

4.8

4.9

4.85


1.622.43 / 23814.85 ± 0.04Triploids

1.603.20 / 31366.41 ± 0.03„G. endtmannii“

1.653.29 / 32246.59 ± 0.04„G. montanum“

1.593.18 / 31166.36 ± 0.04G. argentatum

1.583.17 / 31076.33 ± 0.04G. montanum

1.701.70 / 16663.41 ± 0.02G. flavidum

1.641.64 / 16073.28 ± 0.02„G. endtmannii“

1.631.63 / 15973.27 ± 0.002G. luteum

Genome size (pg)



(pg)

Species

Nuclear genome size determinationNuclear genome size determinationNuclear genome size determinationNuclear genome size determination

G. luteum / G. flavidum

0

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500

750

1000

1250

0 250 500 750 1000

G.l.G.f.


1.622.43 / 23814.85 ± 0.04Triploids

1.603.20 / 31366.41 ± 0.03„G. endtmannii“

1.653.29 / 32246.59 ± 0.04„G. montanum“

1.593.18 / 31166.36 ± 0.04G. argentatum

1.583.17 / 31076.33 ± 0.04G. montanum

1.701.70 / 16663.41 ± 0.02G. flavidum

1.641.64 / 16073.28 ± 0.02„G. endtmannii“

1.631.63 / 15973.27 ± 0.002G. luteum

Genome size (pg)



(pg)

Species

3.7 %


G. argentatum X G. montanum

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„G.m“G.m.


G. argentatum X G. montanum

ConclusionsConclusionsConclusionsConclusions

3x

Sympatric occurence

G. endtmannii

G. flavidum

G. montanum X G. argentatum


Galeobdolon


Galeobdolon

An employment of flow cytometry in the biosystematics of the genus Galeobdolon

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