Equipment (ammonia & nitrate)

Spectrometer

Range : 0- 30 mg/L

Name of model: HACH DR/2010

It’s an instrument used to measure the reflection of light in the material as a function of wavelength.

It was used in the nitrate test.

distilled water

Water was used to clean the conical flask after completing the experiment, it was also used in both experiments to make the equipment zero.

Pipette pumps

Capacity: 2ml

It’s an instrument to dispense small amount of variable, Used to add distilled water to the conical flask

Graduated cylinder

Capacity : 10 ml

Graduated cylinder was used to pour the treated waste water accurately

Scissors

Used to cut the seal on ammonia reagent and nitrate reagent.

Hach DR 2810

Range : 0- 0.50 mg/L

It’s an instrument used to in the ammonia nitrogen test.

Large sample cell

It was used as a closed container to shake the liquids without leaking.

HACH nitrate reagent

For determination of MR and HR nitrate by the NitraVer® 5 cadmium reduction method, It was used in the nitrate test

HACH ammonia reagent

For determination of ammonia by Ammonia Salicylate Reagent Powder Pillows and Ammonia Cyanurate Reagent Powder Pillows, it was used in the ammonia test

Procedure (nitrate)

1. Graduated cylinder was poured with 5ml of treated waste water (s2) and

5ml of distilled water and was poured in a sample cell.

2. The other graduated cylinder was poured with 10ml of treated waste water

(s2) and was poured into sample cell.

3. Reagent was added into the sample cell

4. Equipment hach DR 2010 was switched on and then program 355 and

then 500 wavelength was chosen

5. Timer was started and it was shaked into the larger sample cell for 1 minute

6. After 1minute timer was finished another 5 minutes timer was started but

this time without shacking

7. The sample cell with 5ml of s2 and 5ml of distilled water was inserted into

the equipment and zero was clicked to make the equipment zero

8. prepared sample cell was inserted into the equipment and reading was

taken

9. the procedure was repeated for tape water (s1)

Procedure ( ammonia nitrogen )

1. four sample cell along with two graduated cylinders were prepared

2. 10ml of distilled water was added into 2 sample cells one for diluted and

the other was for tape water

3. 10ml of s2(diluted) was added into 1 sample cell, actual value was equal

to twice dilution factor

4. 5 ml of water with 5 ml of s1(tape water) was added into 1 sample cell

5. Equipment hach DR 2810 was switched on and then program 385 with

wavelength 655 nm was chosen

6. ammonia salicylate reagent was added into all four of the prepared

sample cells

7. Timer was started and it was shaked into the larger sample cell for

3minutes

8. Ammonia cyanurate reagent was added into all four of the sample cells

9. Another timer was started and it was shaked into the larger sample cell

for 15minutes

10.Then the sample cell with distilled water was inserted into the

equipment and zero was clicked to make the equipment zero

11.Diluted sample(s2) cell was inserted into the equipment and reading

was taken

12.Then again the sample cell with distilled water was inserted into the

equipment and zero was clicked to make the equipment zero

13.Tape water(s1) sample cell was inserted into the equipment and

reading was taken