Alternatively activated macrophages derived from monocytes and tissue macrophages are phenotypically

and functionally distinct

by Uma Mahesh Gundra, Natasha M. Girgis, Dominik Ruckerl, Stephen Jenkins, Lauren N. Ward, Zachary D. Kurtz, Kirsten E. Wiens, Mei San Tang, Upal Basu-Roy,

Alka Mansukhani, Judith E. Allen, and P’ng Loke

BloodVolume 123(20):e110-e122

May 15, 2014

©2014 by American Society of Hematology

IL-4c and Thio+IL-4c induces alternatively activated macrophages with distinct phenotypes.

Uma Mahesh Gundra et al. Blood 2014;123:e110-e122

©2014 by American Society of Hematology

Gene expression profiling of monocyte-derived and tissue macrophage-derived AAMs. (A) Unsupervised hierarchical clustering of transcriptional profiles, displayed as a heat map of log-

transformed expression values, from FACS-purified (CD11b+F4/80+) macrophag...

Uma Mahesh Gundra et al. Blood 2014;123:e110-e122

©2014 by American Society of Hematology

GO analysis of transcriptional profiles from monocyte-derived and tissue resident macrophage-derived AAMs. (A) Biological processes (BPs) induced in IL-4c expanded AAM (IL-4c) relative to

resident macrophages (Res). x-axis indicates the amount of statistica...

Uma Mahesh Gundra et al. Blood 2014;123:e110-e122

©2014 by American Society of Hematology

AAMs derived from inflammatory monocytes express PD-L2, CD206, and MHC class II, but not tissue macrophage-derived AAMs. FACS analysis of (A) PD-L2, (B) CD206 and (C) MHC class II

from peritoneal macrophages of mice untreated (naïve/resident) or injected i....

Uma Mahesh Gundra et al. Blood 2014;123:e110-e122

©2014 by American Society of Hematology

Different helminth infections induce either monocyte-derived or tissue-derived AAMs. (A) Representative FACs plots of pleural cavity macrophages on day 12 after L sigmodontis

infection compared with pleural cavity macrophages of mice untreated (Res) or inje...

Uma Mahesh Gundra et al. Blood 2014;123:e110-e122

©2014 by American Society of Hematology

Differential effects of monocyte- and tissue macrophage-derived AAMs on naïve CD4+ T cells.

Uma Mahesh Gundra et al. Blood 2014;123:e110-e122

©2014 by American Society of Hematology

Ucp1 is a unique marker for tissue resident macrophage-derived AAMs. (A) Microarray data for Ucp1 from AAMs. (B) Real-time PCR measurement of Ucp1 expression (relative to GAPDH) in

peritoneal macrophages isolated by adherence.

Uma Mahesh Gundra et al. Blood 2014;123:e110-e122

©2014 by American Society of Hematology