AlleleBiotechnology & Pharmaceuticals, Inc....
4
Allele Biotechnology & Pharmaceuticals, Inc. allelebiotech.com Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645 Fax: (858) 587-6692 [email protected] Copyright ©2008-2010 All ele ustrious pNCS-mTFP1 (E.Coli expression) Catalog Number: ABP-FP-TPNCS10 Size: 10ug Introduction All ele ustrious pNCS-mTFP1 is a bacterial expression vector that constitutively expresses 6xHis-tagged mTFP1 in E. coli. The properties of All ele ustrious mTFP1 are superior to those of the commonly used Aequorea victoria variant ECFP and which can be easily detected in mammalian cells using standard CFP filter sets. In addition, the fluorescent signal from mTFP1 is significantly brighter than that from EGFP using standard GFP filter sets. mTFP1 also makes an excellent fluorescence resonance energy transfer (FRET) donor to yellow or orange fluorescent proteins. Source Engineered variant of Clavularia sp. fluorescent protein cFP484. Recommended Use To further optimize detection, the use of filter sets specific to the mTFP1 excitation and emission spectra is recommended. Features • More than 3-fold brighter than ECFP • More than twice as photostable as ECFP • Single emission peak • Single-exponential fluorescence lifetime (τ = 3.2 ns) • Can be co-imaged with red and yellow FPs • Low sensitivity to acidic pH (fluorescence pKa=4.3) • Mammalian expression vector ready to transfect your favorite cells • True monomer that will not aggregate or cause non-specific interactions Reconstitution 10 µg provided in lyophilized powder form. Reconstitute with 10 µL of nuclease-free water for a final concentration of 1 µg/µL. Storage Store at -80°C for long-term preservation. pNCS-mTFP1 3623bp pUC Ori T7 6X HIS mTFP1 T7 Terminator F1 Origin BLA AMP R T7 Promoter………………..........64-151 6X HIS………………………….....152-250 mTFP1….....................................251-961 T7 Terminator.............................1024-1042 bla Promoter...............................1679-1783 Ampicillin Resistance Gene......1768-2628 pUC Origin..................................2777-3419 Upstream Sequencing Primer: Universal CMV Promoter Primer Downstream Sequencing Primer: SV40 Primer:GCTTT ATTTG TGAAA TTTGT GATGC TATTG C References: Ai HW, Hazelwood KL, Davidson MW, Campbell RE. Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. Nature Methods. 2008 5(5): 401-03. Ai HW, Henderson JN, Remington SJ, Campbell RE. Directed evolution of a monomeric, bright, and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging. Biochem J. 2006. Shaner NC, Steinbach PA, Tsien RY. A guide to choosing fluorescent proteins. Nat Methods. 2005 2(12):905-09
Transcript of AlleleBiotechnology & Pharmaceuticals, Inc....
Allele Biotechnology & Pharmaceuticals, Inc. allelebiotech.com
Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645Fax: (858) 587-6692 [email protected] Copyright ©2008-2010
Alleleustrious pNCS-mTFP1
(E.Coli expression)
Catalog Number: ABP-FP-TPNCS10
Size: 10ug
Introduction
Alleleustrious pNCS-mTFP1 is a bacterial expression vector thatconstitutively expresses 6xHis-tagged mTFP1 in E. coli. The properties ofAlleleustrious mTFP1 are superior to those of the commonly used Aequoreavictoria variant ECFP and which can be easily detected in mammalian cellsusing standard CFP filter sets. In addition, the fluorescent signal frommTFP1 is significantly brighter than that from EGFP using standard GFPfilter sets. mTFP1 also makes an excellent fluorescence resonance energytransfer (FRET) donor to yellow or orange fluorescent proteins.
Source
Engineered variant of Clavularia sp. fluorescent protein cFP484.
Recommended Use
To further optimize detection, the use of filter sets specific to themTFP1 excitation and emission spectra is recommended.
Features
• More than 3-fold brighter than ECFP
• More than twice as photostable as ECFP
• Single emission peak
• Single-exponential fluorescence lifetime (τ = 3.2 ns)
• Can be co-imaged with red and yellow FPs
• Low sensitivity to acidic pH (fluorescence pKa=4.3)
• Mammalian expression vector ready to transfect your favorite cells
• True monomer that will not aggregate or cause non-specificinteractions
Reconstitution
10 µg provided in lyophilized powder form. Reconstitute with 10 µL ofnuclease-free water for a final concentration of 1 µg/µL.
Storage
Store at -80°C for long-term preservation.
pNCS-mTFP1
3623bp
pUC Ori
T76X HIS
mTFP1
T7Terminator
F1Origin
BLAAMPR
T7 Promoter………………..........64-151
6X HIS………………………….....152-250
mTFP1….....................................251-961
T7 Terminator.............................1024-1042
bla Promoter...............................1679-1783
Ampicillin Resistance Gene......1768-2628
pUC Origin..................................2777-3419
Upstream Sequencing Primer:
Universal CMV Promoter Primer
Downstream Sequencing Primer:
SV40 Primer:GCTTT ATTTG TGAAA TTTGT GATGC TATTG C
References: Ai HW, Hazelwood KL, Davidson MW, Campbell RE. Fluorescent protein FRET pairs for ratiometric imaging of dual biosensors. NatureMethods. 2008 5(5): 401-03. Ai HW, Henderson JN, Remington SJ, Campbell RE. Directed evolution of a monomeric, bright, and photostable version of Clavularia cyan fluorescent protein: structural characterization and applications in fluorescence imaging. Biochem J. 2006. Shaner NC, Steinbach PA,Tsien RY. A guide to choosing fluorescent proteins. Nat Methods. 2005 2(12):905-09
Allele Biotechnology & Pharmaceuticals, Inc. allelebiotech.com
SEQUENCE 1 GATCTCGATC CCGCGAAATT AATACGACTC ACTATAGGGA GACCACAACG GTTTCCCTCT 61 AGAATCACCG AGTTTATTCT TGACACCTGA TGCGATGAAT GATATAATAG GAAAGTACTG 121 TTTTGTTTAA CTTTAAGAAG GAGATATACA TATGCGGGGT TCTCATCATC ATCATCATCA 181 TGGTATGGCT AGCATGACTG GTGGACAGCA AATGGGTCGG GATCTGTACG ACGATGACGA 241 TAAGGATCCG ATGGTGAGCA AGGGCGAGGA GACCACAATG GGCGTAATCA AGCCCGACAT 301 GAAGATCAAG CTGAAGATGG AGGGCAACGT GAATGGCCAC GCCTTCGTGA TCGAGGGCGA 361 GGGCGAGGGC AAGCCCTACG ACGGCACCAA CACCATCAAC CTGGAGGTGA AGGAGGGAGC 421 CCCCCTGCCC TTCTCCTACG ACATTCTGAC CACCGCGTTC GCCTACGGCA ACAGGGCCTT 481 CACCAAGTAC CCCGACGACA TCCCCAACTA CTTCAAGCAG TCCTTCCCCG AGGGCTACTC 541 TTGGGAGCGC ACCATGACCT TCGAGGACAA GGGCATCGTG AAGGTGAAGT CCGACATCTC 601 CATGGAGGAG GACTCCTTCA TCTACGAGAT ACACCTCAAG GGCGAGAACT TCCCCCCCAA 661 CGGCCCCGTG ATGCAGAAGA AGACCACCGG CTGGGACGCC TCCACCGAGA GGATGTACGT 721 GCGCGACGGC GTGCTGAAGG GCGACGTCAA GCACAAGCTG CTGCTGGAGG GCGGCGGCCA 781 CCACCGCGTT GACTTCAAGA CCATCTACAG GGCCAAGAAG GCGGTGAAGC TGCCCGACTA 841 TCACTTTGTG GACCACCGCA TCGAGATCCT GAACCACGAC AAGGACTACA ACAAGGTGAC 901 CGTTTACGAG AGCGCCGTGG CCCGCAACTC CACCGACGGC ATGGACGAGC TGTACAAGTA 961 AGAATTCGAA GCTTGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG 1021 CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT 1081 TTTTGCTGAA AGGAGGAACT ATATCCGGAT CTGGCGTAAT AGCGAAGAGG CCCGCACCGA 1141 TCGCCCTTCC CAACAGTTGC GCAGCCTGAA TGGCGAATGG GACGCGCCCT GTAGCGGCGC 1201 ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT 1261 AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG 1321 TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA 1381 CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT 1441 TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG 1501 AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC 1561 GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT 1621 ATTAACGCTT ACAATTTAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT 1681 TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG 1741 CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT 1801 CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA 1861 AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC 1921 GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA 1981 GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC 2041 CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT 2101 ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT 2161 GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC 2221 AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA 2281 CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA 2341 TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG 2401 GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT 2461 AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT 2521 AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA 2581 AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA 2641 GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG 2701 GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC 2761 TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC 2821 GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT 2881 CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT 2941 ACTGTTCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT 3001 ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT 3061 CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG 3121 GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA 3181 CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG 3241 GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG 3301 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC 3361 TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG 3421 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT 3481 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC 3541 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG 3601 CGTTGGCCGA TTCATTAATG CAG
Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645Fax: (858) 587-6692 [email protected] Copyright ©2008-2010
Allele Biotechnology & Pharmaceuticals, Inc. allelebiotech.com
Enzyme Cuts Positions Sequence AatII 1 747 gacgt/c Acc113I 2 116 2074 agt/act Acc16I 2 1160 2332 tgc/gca AccB1I 3 383 1371 2602 g/gyrcc AccB7I 1 690 ccannnn/ntgg AccBSI 3 1271 1716 3517 gagcgg AccIII 1 1104 t/ccgga AcsI 3 962 1595 1606 r/aatty AcyI 3 696 744 2015 gr/cgyc AflIII 1 3443 a/crygt AhdI 1 2555 gacnnn/nngtc Alw21I 3 1887 1972 3133 gwgcw/c Alw44I 2 1883 3129 g/tgcac AlwNI 1 3034 cagnnn/ctg Ama87I 1 527 c/ycgrg ApaLI 2 1883 3129 g/tgcac ApoI 3 962 1595 1606 r/aatty AseI 3 19 2380 3615 at/taat AsnI 3 19 2380 3615 at/taat Asp700I 1 1955 gaann/nnttc AspEI 1 2555 gacnnn/nngtc AspHI 3 1887 1972 3133 gwgcw/c AvaI 1 527 c/ycgrg AviII 2 1160 2332 tgc/gca BalI 1 336 tgg/cca BamHI 1 244 g/gatcc BanI 3 383 1371 2602 g/gyrcc BanII 2 421 1341 grgcy/c BbiII 3 696 744 2015 gr/cgyc BbsI 1 684 gaagac Bbv12I 3 1887 1972 3133 gwgcw/c Bbv16II 1 684 gaagac BcgI 2 219 2039 cgannnnnntgc BcoI 1 527 c/ycgrg BglI 3 818 1170 2437 gccnnnn/nggc BlpI 1 1027 gc/tnagc BpiI 1 684 gaagac BpmI 3 406 769 2470 ctggag Bpu1102I 1 1027 gc/tnagc Bpu14I 1 966 tt/cgaa BpuAI 1 684 gaagac BsaAI 2 718 1412 yac/gtr BsaBI 2 5 243 gatnn/nnatc BsaHI 3 696 744 2015 gr/cgyc BsaI 3 44 274 2488 ggtctc BsaOI 5 1141 2037 2186 3109 3533 cgry/cg BsaWI 4 1104 2259 3090 3237 w/ccggw Bse118I 3 686 1307 2470 r/ccggy Bse8I 2 5 243 gatnn/nnatc BseAI 1 1104 t/ccgga BseRI 2 271 610 gaggag Bsh1285I 5 1141 2037 2186 3109 3533 cgry/cg Bsh1365I 2 5 243 gatnn/nnatc BshNI 3 383 1371 2602 g/gyrcc BsiEI 5 1141 2037 2186 3109 3533 cgry/cg BsiHKAI 3 1887 1972 3133 gwgcw/c BsiI 2 1891 3275 ctcgtg BsiMI 1 1104 t/ccgga BsoBI 1 527 c/ycgrg Bsp119I 1 966 tt/cgaa Bsp13I 1 1104 t/ccgga Bsp1407I 1 951 t/gtaca Bsp143II 5 915 1257 1265 3203 3573 rgcgc/y Bsp1720I 1 1027 gc/tnagc Bsp19I 1 600 c/catgg BspCI 2 1141 2186 cgat/cg BspEI 1 1104 t/ccgga BspHI 2 1715 2723 t/catga BspLU11I 1 3443 a/catgt BsrBI 3 1271 1716 3517 gagcgg BsrBRI 2 5 243 gatnn/nnatc BsrDI 2 2319 2501 gcaatg BsrFI 3 686 1307 2470 r/ccggy BsrGI 1 951 t/gtaca BssAI 3 686 1307 2470 r/ccggy BssSI 2 1891 3275 ctcgtg BssT1I 2 600 1049 c/cwwgg BstBI 1 966 tt/cgaa BstD102I 3 1271 1716 3517 gagcgg BstDSI 2 600 915 c/crygg BstEII 1 895 g/gtnacc BstH2I 5 915 1257 1265 3203 3573 rgcgc/y BstI 1 244 g/gatcc BstMCI 5 1141 2037 2186 3109 3533 cgry/cg BstPI 1 895 g/gtnacc BstSFI 6 32 805 1189 2309 2987 3178 c/tryag BstX2I 10 220 244 864 1107 1908 1925 2693 r/gatcy
2705 2791 2802 BstXI 1 400 ccannnnn/ntgg BstYI 10 220 244 864 1107 1908 1925 2693 r/gatcy 2705 2791 2802 CelII 1 1027 gc/tnagc Cfr10I 3 686 1307 2470 r/ccggy CfrI 4 334 775 2162 3604 y/ggccr Csp45I 1 966 tt/cgaa DraI 3 1977 2669 2688 ttt/aaa DraII 2 474 1054 rg/gnccy DraIII 2 847 1415 cacnnn/gtg DrdI 2 1459 3341 gacnnnn/nngtc DsaI 2 600 915 c/crygg EaeI 4 334 775 2162 3604 y/ggccr Eam1104I 3 1129 1766 3570 ctcttc Eam1105I 1 2555 gacnnn/nngtc EarI 3 1129 1766 3570 ctcttc EclHKI 1 2555 gacnnn/nngtc Eco130I 2 600 1049 c/cwwgg Eco24I 2 421 1341 grgcy/c Eco255I 2 116 2074 agt/act Eco31I 3 44 274 2488 ggtctc Eco57I 4 316 739 1873 2921 ctgaag Eco64I 3 383 1371 2602 g/gyrcc Eco88I 1 527 c/ycgrg Eco91I 1 895 g/gtnacc EcoO109I 2 474 1054 rg/gnccy EcoO65I 1 895 g/gtnacc EcoRI 1 962 g/aattc EcoT14I 2 600 1049 c/cwwgg ErhI 2 600 1049 c/cwwgg Esp1396I 1 690 ccannnn/ntgg FauNDI 1 150 ca/tatg FriOI 2 421 1341 grgcy/c FspI 2 1160 2332 tgc/gca GsuI 3 406 769 2470 ctggag HaeII 5 915 1257 1265 3203 3573 rgcgc/y Hin1I 3 696 744 2015 gr/cgyc HincII 1 790 gty/rac HindII 1 790 gty/rac HindIII 1 969 a/agctt Hsp92I 3 696 744 2015 gr/cgyc Kpn2I 1 1104 t/ccgga Ksp632I 3 1129 1766 3570 ctcttc LspI 1 966 tt/cgaa MamI 2 5 243 gatnn/nnatc MflI 10 220 244 864 1107 1908 1925 2693 r/gatcy 2705 2791 2802 MluNI 1 336 tgg/cca MroI 1 1104 t/ccgga MroNI 1 1307 g/ccggc MscI 1 336 tgg/cca MslI 5 177 599 1784 2143 2302 caynn/nnrtg Msp17I 3 696 744 2015 gr/cgyc MspA1I 4 1026 1919 2860 3105 cmg/ckg NaeI 1 1309 gcc/ggc NcoI 1 600 c/catgg NdeI 1 150 ca/tatg NgoAIV 1 1307 g/ccggc NgoMI 1 1307 g/ccggc NheI 1 188 g/ctagc NspBII 4 1026 1919 2860 3105 cmg/ckg NspI 1 3447 rcatg/y NspV 1 966 tt/cgaa PflMI 1 690 ccannnn/ntgg Ple19I 2 1141 2186 cgat/cg PshBI 3 19 2380 3615 at/taat Psp1406I 2 1953 2326 aa/cgtt PspEI 1 895 g/gtnacc PstNHI 1 188 g/ctagc PvuI 2 1141 2186 cgat/cg RcaI 2 1715 2723 t/catga SapI 1 3571 gctcttc ScaI 2 116 2074 agt/act SfcI 6 32 805 1189 2309 2987 3178 c/tryag SfuI 1 966 tt/cgaa SspBI 1 951 t/gtaca SspI 2 1620 1750 aat/att StyI 2 600 1049 c/cwwgg Van91I 1 690 ccannnn/ntgg VneI 2 1883 3129 g/tgcac VspI 3 19 2380 3615 at/taat XbaI 1 58 t/ctaga XcmI 1 937 ccannnnn/nnnntgg XhoII 10 220 244 864 1107 1908 1925 2693 r/gatcy 2705 2791 2802 XmnI 1 1955 gaann/nnttc
The following enzymes do not cut:
AatI, Acc65I, AccI, AclNI, AfeI, AflII, AgeI, AocI, Aor51HI, ApaI, AscI, Asp718I, AspI, AtsI, AvrII, BanIII, BbeI, BbrPI, BbuI, BclI, BfrI, BglII, BlnI, Bsa29I, BsaMI, BscI, Bse21I, BseCI, BsePI, BsgI, BsiWI, BsmBI,
BsmI, Bsp106I, Bsp120I, Bsp68I, BspDI, BspMI, BspTI, BspXI, BssHII, Bst1107I, Bst98I, BstSNI, BstZI, Bsu15I, Bsu36I, CciNI, Cfr42I, Cfr9I, ClaI, CpoI, CspI, CvnI, EagI, Ecl136II, EclXI, Eco105I, Eco147I,
Eco32I, Eco47III, Eco52I, Eco72I, Eco81I, EcoICRI, EcoNI, EcoRV, EcoT22I, EheI, Esp3I, FbaI, FseI, HpaI, KasI, KpnI, Ksp22I, KspI, MfeI, MluI, Mph1103I, MspCI, MunI, Mva1269I, NarI, NotI, NruI, NsiI,
PacI, PaeI, PaeR7I, Pfl23II, PinAI, PmaCI, Pme55I, PmeI, PmlI, Ppu10I, PpuMI, PshAI, Psp124BI, Psp5II, PspAI, PspALI, PspLI, PspOMI, PstI, PvuII, RsrII, SacI, SacII, SalI, SbfI, SexAI, SfiI, Sfr274I,
Sfr303I, SgfI, SgrAI, SmaI, SmiI, SnaBI, SpeI, SphI, SplI, SrfI, Sse8387I, SseBI, SstI, SstII, StuI, SunI, SwaI, Tth111I, Vha464I, XhoI, XmaI, XmaIII, Zsp2I
Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645Fax: (858) 587-6692 [email protected] Copyright ©2008-2010
Allele Biotechnology & Pharmaceuticals, Inc. allelebiotech.com
Related products: Current Alleleustious Fluorescent Protein Family Members: The founding member is mTFP1. mWasabi is a green derivative with 4 amino acid difference from mTFP1. mTFPG3 is a green FP with 3 amino difference from mTFP1. It has a slightly red-shifted emission spectrum and is 1.5 fold brighter compared to EGFP. While being very bright, mTFPG3 can be photobleached within ~5 sec, about 30 times faster than EGFP, suitable for certain cell-based assays that require a bright FP with very short half-life. mTFP0.7 is a precursor during the evolution of mTFP1. It has photo-switchable properties like Dronpa that cycles between fluorescent and nonfluorescent states. It may be developed into components in PALM/SIM applications.
Basic Vectors Three vectors are available: pNCS-mTFP1, pmTFP1-N and pmTFP1-C. Subcellular Marker Vectors Twenty six vectors are available. Vectors in Viral Vectors All plasmid format vectors in Allele's Phoenix Retroviral vector or HiTiter Lentiviral Vectors.
IMPORTANT NOTICE TO PURCHASER: This product may be subjects of pending U.S. and foreign patents. Allele Biotech grants academic/government research institutions a worldwide, non-exclusive, royalty-free, limited license to use this product for non-commercial life science research use only. Such license specifically prohibits the right to sell or otherwise transfer this product or its derivatives to a third party. Industry For-Profit Institutions: Institutions that wish to use this product are required to obtain a sub-licensing agreement from Allele Biotech. For more information please visit Fluorescent Protein Sublicense, or contact us via phone or email.
Allele Biotechnology and Pharmaceuticals 9924 Mesa Rim Road San Diego, CA 92121 Tel: (800) 991-RNAi (858) 587-6645Fax: (858) 587-6692 [email protected] Copyright ©2008-2010
