7/27/2019 Alcoholic Fermentation Laba

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 Name: _______________________________________________ Date: __________________ Class: ______ 

ALCOHOLIC FERMENTATION LAB

Introduction

Alcoholic fermentation is a pathway for anaerobic respiration done by yeast cells to produce energy from a sugar 

source. During alcoholic fermentation, the products are 2 ATP molecules, ethyl alcohol, and carbon dioxide. This small

amount of energy is not sufficient to maintain larger cells, but it is enough for the small yeast cells to be able to live and

reproduce.

Materials

  Apple Juice  Active Yeast

  Test tube

  Sugar 

  Masking tape

  Balloon

  Ruler 

Procedure

1.  Obtain a test tube from the back. Make sure the test tube is clean. If it isn’t, clean it thoroughly. Dirt may cause

an infection, which will cause the yeast to die.

2.  Each individual should get a graduated cylinder and fill their test tube up with 15mL of grape juice from the

 beaker. Make sure you get exactly 20mL of juice.  Each individual will have their own test tube.

3.  Add 3 pinches of sugar to your test tube. (What is the sugar for?)

4.  Ask Mr. Hinnenkamp to add enough yeast to the test tube to cover the liquid. Then, swish around the juice to mix

well.

5.  Take a balloon and stretch it over the end of the test tube, making sure it is tightly secured.

6.  Using a ruler, measure the diameter and circumference of the balloon in millimeters. Record this data.

7.  Put a piece of tape around the balloon to keep it from flying off. Also, put your name on the balloon.

8.  We will take measurements for the next few days.

9.  Clean up! If the room is not clean of all messes, we will be cleaning tomorrow!

7/27/2019 Alcoholic Fermentation Laba

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 Name: _______________________________________________ Date: __________________ Class: ______ 

FERMENTATION LAB WRITE-UP**REFER TO GENERAL LAB WRITE UP**

Purpose: As always state the purpose and give some background information. This should be 2 separate sections.

Procedure: Summarize the procedure.

Data/Results: Use the table below to show the data collected from the measurements of the balloon. Also, using the measurements

from the diameter, you should mathematically calculate the circumference of the balloon. Be sure to show the work for all of the

calculations. The calculations can be hand written on the report.

Time

Diameter Measured

(mm)

CircumferenceMeasured

(mm)

CircumferenceCalculated(c= )

Day#1

Day#2

Day#3

Day#4

Include a couple sentences where you talk about how accurate your measurement of the circumference was based on the mathematical

calculations using the measured diameter.

You will also make 3 graphs. 1.) A line graph; circumference measured vs time.

2.) A line graph; diameter measured vs time.

3.) A line graph, circumference calculated vs time.

Remember, to make a line graph on excel, you must choose the xy scatter. You may make one graph with all three lines instead of 3individual graphs

Conclusion: As always, include your sources of error.

Questions

1.  What material filled up the balloon? Use complete sentences.

2.  What was the purpose of the sugar added to the grape juice?

3.  Describe in detail the other form of anaerobic respiration.

4.  Where is the energy produced during anaerobic respiration?

5.  What type or respiration does the electron transport chain get involved in, and briefly describe how it works.

Did you include…………  Place a check in each circle if you included that item in your lab report.

o  Title of the lab

o  Purpose

o  Background information (at least half a page)

o  Procedure

o  Data table (with work)

o  Accuracy of your measurements

o  3 line graphs

o  3 sources of error 

o  5 conclusion questions

o  Did you type the lab?

o  This sheet stapled to the end of the lab