Alcohol has the largest death rate out of all other drugs
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Transcript of Alcohol has the largest death rate out of all other drugs
Alcohol has the largest death rate out of all other drugs
Disorders caused by alcohol have increased over the past 6 years
Null Hypothesis: the ethanol exposure will have no effect on the short term memory of
Drosophila melanogaster.
Alternate Hypothesis: The Drosophila exposed to ethanol before training will have impaired memory and the flies exposed to alcohol after training will not have damaged memory. The control group will form short term memory
The purpose of this experiment is to demonstrate the effects of alcohol exposure on short term memory in
drosophila.
AlcoholShort Term Memory
Short-term memory is stored in the α/β mushroom body neurons
Olfactory training
This diagram shows how alcohol effects the brain
This graph shows the results of the two different groups. The chpd flies’ short-term memory didn’t last as long as the control group
Comparison Between Control and chpd Drosophila
Need
Knowledge Base
Literature Review
Purpose
Hypothesis
Moore, 98Moore, 98
LaFerriere, 07LaFerriere, 07
AbstractAbstract
GoesGoes
HereHere
The Heat Box
A model of the heat-box used to train short-term memory
Results
Time interval results
This diagram shows the process of short term memory in the brain
Deaths From Disease
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Figure 7Figure 8
The Effects of Alcohol on Short Term Memory in Drosophila
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Groups
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Wild Type Drosophila
Control
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Alcohol Before
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Alcohol After
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Exposed to Alcohol for 5 minutes
Trained using olfactory
Trained using olfactory
Trained using olfactory
Exposed to Alcohol for 5 minutes
Tested 5 hours laterTested 5 hours laterTested 5 hours later
Methodology
Results
Performance index used to see if learning occurred
Performance Index: (Correct-Incorrect)-.5/.5 x100
Testing Chamber
Flies are lowered between the familiar and unfamiliar scents and are given 4 minutes to choose
Training Chamber
Flies are shocked while simultaneously being being pumped an odor.
Drosophila Learning Without Middle Flies
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Figure 11 Figure 12
Results are put through the performance index with the flies that moved to the unfamiliar scent are correct and those that stayed in the middle or moved to the familiar scent were incorrect
Results are put through the performance index with the flies that moved to the unfamiliar scent are correct and those that moved to the familiar scent were incorrect and those that stayed in the middle were discounted
Data supported part of the alternate hypothesis which stated that the flies exposed to ethanol before training will have impaired memory.
Effects of alcohol on long term memory.Effects of sleep deprivation on short-term memory.Effects of resveratrol on short-term memory.Effects of alcohol on short-term memory in ADHD flies
Bayard, Max. "Alcohol Withdrawal Syndrome - March 15, 2004 - American Family Physician." American Academy of Family Physicians. 2004. 25 May 2009 <http://www.aafp.org/afp/20040315/1443.html>.
Ericson, Nels. "Substance Abuse:The Nation’s Number One Health Problem." 2001. U.S. Department of Justice. <http://www.ncjrs.gov/pdffiles1/ojjdp/fs200117.pdf>.
Farr, Susan. "Drinking for just eight weeks impairs learning and memory in mice." Science, Industry and Business: the innovations-report. 15 May 2009 <http://www.innovations-report.com/html/reports/life_sciences/report-45402.html>.
Grant B, Harford TC, Dawson DA, et al. Prevalence of DSM-IV alcohol abuse and dependence, United States 1992. Alcohol Health & Research World 18(3). 1994.
Laferriere, Holly, and Douglas J. Guarnieri. "Genetic Dissociation of Ethanol Sensitivity and Memory Formation in Drosophila melanogaster." (2008). Genetics. <http://www.genetics.org/cgi/content/full/178/4/1895/>.
Miller, Loren L., and Michael P. Dolan. "SpringerLink - Journal Article." SpringerLink Home - Main. 1973. <http://www.springerlink.com/content/u0161w1781274g83/>.
Moore, Monica, and Jim DeZazzo. "Ethanol Intoxication in Drosophila:Genetic and Pharmacological Evidence for Regulation by the cAMP Signaling Pathway." (1998). <http://www.northwestern.edu/neurobiology/faculty/allada2/gbbpapers/MooreHeberlein98.pdf>.
The number of trials for drosophila exposed to alcohol could have been higher and possible human errors.
Ethanol beaker
Air pump
Alcohol Exposure
Exposure chamber
The purpose of this experiment is to demonstrate the effects of alcohol exposure on short term memory in drosophila.
Results demonstrated that alcohol exposure before and after had similar results.
When graphed with out the undecided flies results appeared similar with the before and after groups varying by a slight percent
Discussion
Limitations Conclusion
Future Studies
Bibliography
Flies are exposed to 200 Proof ethanol for 5 minutes before or after training
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