Albumin Analysis by Potentiometric Method

Properties of Albumin

• Half-life: 17 to 23 days

• Molecular weight: 68,000

• Isoelectric point: 4.8

The Advantages of PotentiometricMethod

• 1. Integrate easily

• 2. Microfabricate easily

• 3. Real time response

Materials

• Monomer Dimethylaminoethyl methacrylate-methyl

chloride:DMAEMMC Dimethylaminopropyl

methacrylamide:DMAPMA• Crosslinking agent Tetraethylene glycol

dimethacrylate:TEGDMA

Materials

• Initiators

Cumene hydroperoxide:CHP

Tetramethyl ethylenediamine:TMEDA

• Modification agent

2-mercapto ethanol

3-mercapto propionic acid

n-dodecyl thiol

Materials

• Electrodes:

Working : Au polymer film

Counter: Pt

Reference: Ag/AgCl

• Electrolyte:

0.5M KCl