Alaska King Crab Research

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    Alaska King Crab Research,

    Rehabilitation & Biology Program

    An Introduction

    Presented by Jeff HetrickDirector Alutiiq Pride Shellfish

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    The AKCRRAB Science Team

    Ginny Eckert UAF/UAS

    Brad Stevens U. Mass. Dartmouth

    Brian Allee Alaska Sea Grant

    Jeff Hetrick Alutiiq Pride Shellfish Hatchery

    Sara Persselin NOAA NMFS

    Celeste Leroux UAF/Alaska Sea GrantBen Daly Alaska Sea Grant

    Jim Swingle Alaska Sea Grant

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    Background Red King Crab Fishery

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    Background Blue King Crab Fishery

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    Early Enhancement Activities in Alaska

    1992 International King Crab

    Rehabilitation and EnhancementSymposium

    1990s-2000s Extensive researchby Dr. Brad Stevens in Kodiakand Japan

    2000s Continued research byNMFS Kodiak

    2006 Alaska Crab StockEnhancement and RehabilitationWorkshop

    Small scale experiment setup

    S. Persselin and B. Stevens, Kodiak A

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    AKCRRAB - Structure

    Steering Committee

    Science Team

    Partnerships

    Research collaborators

    Advisory Board

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    Crab and Lobster Enhancement Initiatives-A Few Examples-

    Swimming crab, Japan

    Red King Crab, Japan

    American Lobster, New England

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    Crab and Lobster Enhancement Initiatives-A Few Examples-

    Blue crab, Chesapeake Bay Excellent model for

    investigating feasibility ofenhancement

    European lobster, NorwayCommercial sea ranchingvery successful

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    Prezoea

    Crab Instar 1Glaucothoe

    Zoea 4Zoea 3Zoea 1 Zoea 2

    Egg Day 1 Egg Day 395

    King Crab Life Cycle

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    King Crab Life Cycle

    Female with extruded eggsMating behavior

    Juvenile podding behavior Adult solitary behavior

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    Broodstock Collection

    St. Paul, Pribilof Islands

    November 2006Alitak Bay, Kodiak

    July 2006

    Anchorage

    Seward

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    Hatchery Setup

    Alutiiq Pride Shellfish HatcherySeward Marine Center

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    Hatchery Setup Larval culture

    Larval Rearing Modules at APSH

    Flow-through system

    Conical bottom tanks Circulation with air stones Uniform lighting of tanks

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    Larval Rearing Food Production

    Algae production

    24 hr. oldArtemia nauplius

    Artemia sp. production

    Isochrysis galbana algae

    Artemia sp. (brine shrimp) Algae

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    AKCRRAB 2007 Experiments

    Red King Crab Larval diet study

    Larval density hatcherytrials

    Blue King Crab Larval diet/handling

    stress study

    Larval density study

    Juvenile trial rearing

    Water quality investigation

    P. Platypus ZIs swim in Petri dish

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    RKC Larval Rearing Diet StudyMarch 7, 2007 May 3, 2007

    Purpose:

    To test effect of algae or diatom enrichmenton RKC larval survival and growth toglaucothoe stage.

    Diet Treatments:

    Isochrysis galbana Tahitian strian (T-ISO)

    Pavlova lutheri(PAV) Thalassiosira pseudonana (3H)

    No enrichment,Artemia sp. only (Art.)

    P. camtschaticus ZI

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    BKC Larval Handling Stress/Diet studyMay 14, 2007 June 12, 2007

    Purpose:To test effects of live algae vs. algae

    concentrate as dietary enrichmentand effect of handling stress onBKC larval survival and growth tothe glaucothoe stage.

    Treatments:

    Diet: Live T-ISO

    Concentrate T-ISO

    Handling: Once per zoeal stage

    No handling

    P. platypus ZIs

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    BKC Larval Rearing - Survival

    Percent survival per diet treatment

    -handling treatment only-

    0

    10

    20

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    50

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    Z1 Z2 Z3 Z4 G

    MEAN LIVE MEAN CONCENTRATE

    Percent Survival of all BKC ZI to G

    0

    20

    40

    60

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    100

    Z1 G

    Handling, live algae No handling, live algae

    Handling, algae concentrate No handling, algae concent

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    RKC & BKC Growth

    Average dry weight of larvae

    0.000

    0.200

    0.400

    0.600

    0.800

    1.000

    1.200

    ZI ZII ZIII ZIV

    We

    ightinmilligrams

    RKC BKC

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    Average Temp. of RKC Larval Rearing Tanks

    3

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    10

    Duration of experiment

    DegreesCelcius

    Average temperature of BKC larval rearing tanks

    3

    5

    7

    9

    11

    13

    Duration of experiment

    DegreesCelcius

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    Larval Rearing Survival - Discussion

    Concern Course of Action

    Water Quality Monitor for hydrocarbons

    Microbiological contamination Monitor forVibrio bacteria

    Tem erature fluctuation Im rove water s stem

    Excessive aeration Decrease aeration

    Nutritional deprivation

    Fatty acid analysis &

    Further diet investigations

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    Other Hatchery Studies

    Larval rearing density studies

    Water quality investigations

    Juvenile trial rearing

    Pathology & Genetics Larval fatty acid analysis

    Larval growth measurements

    Ongoing Projects

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    2007 Research Findings Summary

    Hatchery scale red and blue king crab larval and juvenile

    rearing is possible.

    Tremendous accomplishment in areas of technologytransfer, hatchery setup, preliminary research andbaseline data.

    Need to refine setup in identified ways to improvesurvival, as expected.

    P. camtschaticus glaucotho

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    AKCRRAB Long-term vision

    Artificial PropagationHabitat Studies

    Genetics & Pathology

    Goal: Assess biological & economicfeasibility of red and blue king crabpopulation rehabilitation through oceanranching.

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    The goal of this three year grant would be the development of productionand operation techniques (hatchery and nursery) with a positive

    benefits/cost for producing juvenile king crab for release into the wild fromfacilities at Old Harbor

    ANA King CrabRehabilitation/Enhancement Grant

    Goal and Objectives

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    1) Investigate and develop king crab hatchery/nursery techniques at

    the Alutiiq Pride Shellfish Hatchery in Seward for producing king crabjuveniles with a 1 inch carapace length. This would include apreliminary marking program for identifying hatchery released crabrecovered in the wild.

    2) Training for Old Harbor residents. This would include training for upto six residents in juvenile king crab hatchery and nursery productiontechniques to a level that would allow them to operate ahatchery/nursery system in Old Harbor under general supervision, andtraining for up to three residents in supervision and managementduties and responsibilities. This would include training for at least oneresident to become a certified diver who would assist in variousevaluation work associated with the project such as habitat surveys,identifying potential release sites and monitoring the progress of

    released crab.

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    3) Identify potential sites for a juvenile king crab production

    facility in Old Harbor and produce design criteria forconstructing and equipping a hatchery/nursery system in OldHarbor with low capital and operating costs.

    1) Conduct a benefit/cost analysis forhatchery/nursery production of juvenile king crab atOld Harbor.

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    Thanks

    Alaska Sea Grant College Program AKCRRAB Science Team & Steering Committee

    NOAA Aquaculture Program

    NOAA National Marine Fisheries Service

    UAF School of Fisheries and Ocean Sciences

    Alutiiq Pride Shellfish Hatchery

    Alaska Department of Fish and Game United Fishermans Association

    Central Bering Sea Fishermans Association

    United Fishermans Marketing Association

    and all of our supporters nationwide

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    Questions

    Red king crab (P. camtschaticus) eggs, Seward, Alaska