Cell differentiation basing on ACTB expression

AIM• Detect mouse and human ACTB gene in two co-

cultured cell liens

Cell Lines:MEF – Mus muscullus embroynic fibroblasts

Cells infected with SV40 polyomavirus. LargeT antigen(protein) is believed to bind and limit p53 activity – transcription factor responsible for cell death

AIM• Detect mouse and human ACTB gene in two co-

cultured cell liens

Cell Lines:BjhTERT – Homo sapiens sapiens fibroblast

immortalised with hTERT (human telomere reverse transcriptase)

CCTCAATGCTGCTGCTGTACTAC

TGCGTCTATTTAGTGGAGCC

Cy3

Cy5

AGCCTCGCCTTTGCCTTCCTTTTACGACCTCAATGCTGCTGCTGTACTACTCTTCGCCCCGCGAGCACAG TTACACCCTTTCTTTGACAATCCGAGTAGTCTTTGTGCGTCTATTTAGTGGAGCCGCAACTATCTTCTTTGACTGTTACTGAGCTGCGTT

No primer given for mouse ACTB padlock. We will use random priming ! (random decamers : 10 bases oligos)