Metabolic synchronization of the liver circadian clock - eDiss - Georg
AGING AND THE CIRCADIAN CLOCK DENNIE KIM MCB186 13 DECEMBER 2006.
-
date post
19-Dec-2015 -
Category
Documents
-
view
213 -
download
0
Transcript of AGING AND THE CIRCADIAN CLOCK DENNIE KIM MCB186 13 DECEMBER 2006.
![Page 1: AGING AND THE CIRCADIAN CLOCK DENNIE KIM MCB186 13 DECEMBER 2006.](https://reader030.fdocuments.in/reader030/viewer/2022032703/56649d2a5503460f949fe5a9/html5/thumbnails/1.jpg)
AGING AND THE CIRCADIAN CLOCK
DENNIE KIMMCB186
13 DECEMBER 2006
![Page 2: AGING AND THE CIRCADIAN CLOCK DENNIE KIM MCB186 13 DECEMBER 2006.](https://reader030.fdocuments.in/reader030/viewer/2022032703/56649d2a5503460f949fe5a9/html5/thumbnails/2.jpg)
LEVELS OF CONTROL
CHOU ET AL., 2003. J. NEURO 23(33)
![Page 3: AGING AND THE CIRCADIAN CLOCK DENNIE KIM MCB186 13 DECEMBER 2006.](https://reader030.fdocuments.in/reader030/viewer/2022032703/56649d2a5503460f949fe5a9/html5/thumbnails/3.jpg)
CR, SIRT1, and NEURONAL CELL SURVIVAL
B.L. Tang, 2006. Neurobiology of Aging 27 (503)
![Page 4: AGING AND THE CIRCADIAN CLOCK DENNIE KIM MCB186 13 DECEMBER 2006.](https://reader030.fdocuments.in/reader030/viewer/2022032703/56649d2a5503460f949fe5a9/html5/thumbnails/4.jpg)
DETERIORATION OF BIOLOGICAL RHYTHMS
NEURODEGENERATION ?
Bentivoglio et al. 2006
![Page 5: AGING AND THE CIRCADIAN CLOCK DENNIE KIM MCB186 13 DECEMBER 2006.](https://reader030.fdocuments.in/reader030/viewer/2022032703/56649d2a5503460f949fe5a9/html5/thumbnails/5.jpg)
DETERIORATION OF BIOLOGICAL RHYTHMS
Hofman et al., 2006
![Page 6: AGING AND THE CIRCADIAN CLOCK DENNIE KIM MCB186 13 DECEMBER 2006.](https://reader030.fdocuments.in/reader030/viewer/2022032703/56649d2a5503460f949fe5a9/html5/thumbnails/6.jpg)
NOT JUST IN THE SCN?
M. HOFMAN, 2000.
![Page 7: AGING AND THE CIRCADIAN CLOCK DENNIE KIM MCB186 13 DECEMBER 2006.](https://reader030.fdocuments.in/reader030/viewer/2022032703/56649d2a5503460f949fe5a9/html5/thumbnails/7.jpg)
CR-INDUCED LIFESPAN EXTENSION
- and -
NEURONAL RESCUE?
![Page 8: AGING AND THE CIRCADIAN CLOCK DENNIE KIM MCB186 13 DECEMBER 2006.](https://reader030.fdocuments.in/reader030/viewer/2022032703/56649d2a5503460f949fe5a9/html5/thumbnails/8.jpg)
Hypothesis 1: CR mice are protected from degeneration
of rhythmicity.
• Calorie restrict miceCONTROL(S): NORMAL DIET MOUSE, RESVERATROL-TREATED MOUSE, SIRT1-K/O MOUSE
• Measure rhythms/neuronal activity• Test entrainment
![Page 9: AGING AND THE CIRCADIAN CLOCK DENNIE KIM MCB186 13 DECEMBER 2006.](https://reader030.fdocuments.in/reader030/viewer/2022032703/56649d2a5503460f949fe5a9/html5/thumbnails/9.jpg)
Hypothesis 2: Deterioration of Circadian Clock is a Problem of
Neurodegeneration
• If neurons of the SCN (or DMH/VLPO) die during aging, the number of connections made should decrease.
• Use retrograde/anterograde tracers to label neuronal connections in young and old mice.
• Similarly, compare old mice to CR “old” mice.
![Page 10: AGING AND THE CIRCADIAN CLOCK DENNIE KIM MCB186 13 DECEMBER 2006.](https://reader030.fdocuments.in/reader030/viewer/2022032703/56649d2a5503460f949fe5a9/html5/thumbnails/10.jpg)
Hypothesis 3: SIRT1 Activation in SCN Neurons
Can Prevent Circadian Dysfunction.
• Create an temporally/transiently SIRT1-inducible transgenic mouse.
• Measure rhythms/SCN-neuronal activity compared to wt mouse.
• Western blot/In situ hybridization/Immunohistochemistry to show increased levels of SIRT1 in transgenic mouse.