Agilent 1100 Series LC/MSD Brochure (5968-9804E)chemistry.gsu.edu/files/2014/05/1100s_bro.pdfThe...
Transcript of Agilent 1100 Series LC/MSD Brochure (5968-9804E)chemistry.gsu.edu/files/2014/05/1100s_bro.pdfThe...
Agilent 1100 Series LC/MSD
New standards for valueand performance
Analyze a wide variety of samples
Standard positive and negative ionizationmodes, and a choice of electrospray ion-ization (ESI) or atmospheric-pressurechemical ionization (APCI), allow you toanalyze a wide variety of compoundssuch as:
Drugs and drug metabolites
Proteins and peptides
Pesticides and herbicides
The advantages of mass spectral data—made easy and reliable
These results from the analysis of a sulfadrug mixture show the complementary combination of mass chromatograms, UVchromatograms, and spectral information.
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The Agilent 1100 Series LC/MSD offers you the sensitivity, selectivity,and depth of molecular information inherent in mass spectrometry—ina package that is rugged, reliable, and surprisingly easy to learn, operate,and maintain.
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Increase confidence in results andshorten analysis times
The LC/MSD provides molecular weightand structural information that comple-ments the spectral information from adiode-array UV-Visible detector. Theseorthogonal sets of data provide confidentcompound identification from a singlechromatographic run—in one report.
You do not need to derivatize analyteslacking chromophores to obtain data
Mass spectral data make it easy to detectand identify low-level impurities
The ability to quantitate using unique masses means quantitative performance is less affected by matrixinterference and unresolved chromatographic peaks
Fully-resolved peaks are not required formass spectral analysis; allowing you toshorten chromatographic runs andreduce sample preparation
Reproducible in-source fragmentationcan confirm sample identity
Use existing HPLC methods
With its patented orthogonal-spray ionsource and high-capacity drying gas system, the LC/MSD provides excellentsensitivity and reproducibility over a widerange of LC operating conditions. Thisminimizes the need to alter establishedLC methods for LC/MS analyses.
Flow Rates
Up to 1.0 ml/min ESIUp to 1.5 ml/min APCI
Solvent Composition
100% aqueous to 100% organic
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Save time with fast, easy system setup
Automated tuning and a built-in calibrantdelivery system make system setup fastand easy, while ensuring the highest sensitivity and reproducibility.
Calibrant delivery is automatic, without replumbing
Pre-made calibrants save time and avoid mistakes
Easy-to-use software allows you to man-ually tune the instrument when desired
Autotune and automated calibrant deliveryshorten system setup time and ensure the high-est sensitivity and day-to-day reproducibility.
Automated switching improves performance
The LC/MSD’s automated switching valvesimplifies plumbing and provides a singleLC connection. This reduces leakage,minimizes extra-column band broadening,and allows you to divert the void volumeas part of a method.
An optional, integrated manual injectionvalve gives you a way to quickly introducesamples that do not require separation.
Switch from ESI to APCI in just a few minutes
A hinged, swing-out spray chamberspeeds switching between modes andmakes cleaning easier.
Improve uptime with easy maintenance
The LC/MSD is designed to minimizemaintenance requirements and makewhat maintenance is required fast andeasy.
Orthogonal spray orientation keeps thecapillary and ion optics cleaner
Large sampling orifice prevents capillaryplugging
Slide-out, self-aligning components suchas the sprayer make maintenance easier
Diagnostic software can log the hours ofsystem use and prompt you when keycomponents are due for servicing
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Analyses at low picogram levels are common thanks to Agilent’spatented orthogonal-spray ion source design and high-capacity dryinggas system. Data are exceptionally reproducible, with RSDs of lessthan 5 percent. For quantitative performance, response is linear overseveral orders of magnitude.
Gain the advantages of orthogonal spray...
Agilent revolutionized electrospray technology, replacing traditional in-linesource geometry with a fixed-positionsprayer orthogonal to the mass analyzer’soptical axis. This approach:
Greatly reduces background from solventclusters and mobile-phase adducts
Lets you optimize both desolvation andion transport for improved sensitivity
Substantially reduces ion source contamination and the need for cleaning
Eliminates the need to adjust the positionof the sprayer—even when the flow rateor solvent composition changes
Achieve the highest sensitivity and reproducibility
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HPLC inlet
Skimmer
Capillary
Octopole
Quadrupole
Lenses
Fragmentationzone (CID)
Sprayer
Orthogonal spray orientationand high-capacity drying gassystem improve instrumentperformance and decreasemaintenance requirements.
Orthogonal-spray ion source isvery tolerant of non-volatile com-ponents in samples or mobilephase. This ion source was stillgenerating useful data.
Swing-out spray chambermakes maintenance easier.
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3 Torr
1 x 10-5 Torr
...excellent sensitivity...
The LC/MSD delivers excellent sensitivityin all operating modes. And the extraspecificity provided by mass spectraldata makes that sensitivity more useful.
When scanning a range of masses,LC/MSD sensitivity is comparable to thesensitivity of a good diode array UV detector
Sensitivity is enhanced 5 to 100 timeswhen the LC/MSD is set to monitor onlyselected masses characteristic of thecompounds being analyzed (selected ionmonitoring mode)
With its improved ion optics, sensitivity ofthe LC/MSD SL model is 10 times greaterthan the sensitivity of previous LC/MSDs
Five replicate injections of 1 picogram of reserpine on column inESI mode (SL model).
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APCI
Caffeine reproducibility in ESI and APCI modes of operation
Standard Deviation = 0.01Average= 0.953
Standard Deviation =0.008Average = 0.945
Reproducible dataincrease confidence in methods and results.
Added sensitivity extends linear range to improve quantitation results.
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...and enhanced stability and reproducibility
Good reproducibility yields better qualita-tive and quantitative results. The LC/MSDexhibits exceptional stability and repro-ducibility, giving you more confidence inthe validity of your methods and data.
The VL model can provide linear quanti-tation results over three orders ofmagnitude. The SL model is even better:it can provide linear quantitation resultsover four orders of magnitude.
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Simplify operation
The Microsoft® Windows® -based userinterface makes the software easy tolearn and operate.
System control and data acquisitionparameters require only two windows
Some parameters are calculated auto-matically. For example, if you provide thechromatographic peak width, the systemautomatically optimizes the data acquisition rate
Other parameters are preset or reliabledefaults are provided
Online help includes tutorials to lead youthrough tasks. Digital videos show you howto perform common maintenance tasks
Acquire more data quickly and easily
The software is fully inte-grated with the Agilent LC3D ChemStation software.
In this example, the FIA series table was used to make repeatedflow injections of a caffeine standard while the fragmentor voltagewas varied.
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The LC/MSD ChemStation software is fully integrated with our LC 3DChemStation software. Thanks to an easy-to-use graphical user inter-face and powerful automation features, both new and experiencedusers can quickly take advantage of the LC/MSD’s capabilities.
Speed analyses
Automated flow-injection analysis (FIA)allows you to quickly analyze samplesthat do not require separation. When youare developing methods, FIA can help youfind the best settings for MS parameters.Setup is easy using the FIA series table.
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Maximize the information from each analysis
The multisignal analysis found in theLC/MSD SL model gives you the power toacquire up to four separate signals, eachusing a different acquisition mode, from asingle sample injection. Choose positive ornegative ionization, selected ion monitoring(SIM) or scanning, and fragmentor voltage.
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Maximize the information from asingle analysis by using up tofour different acquisition modes.
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Acquiring positive and negative ionshelps you determine which mode pro-duces the best response for a particularcompound. And seeing both [M+H]+ and[M-H]- ions can confirm molecular weight
Combining SIM and scan data meansthat, from the same run, you get theutmost sensitivity for quantitation of low-level target compounds and completespectra for other high-level compoundsand unknowns
Combine the power of LAN and the Intranet
The Agilent ChemStation software is compatible with the latest in networkingtechnologies.
Instruments can be controlled, and theiroperating status checked, from any PC—even if it is not in the laboratory
Complete web-page-ready reports canbe sent directly to your desktop
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Acquiring data with low and high frag-mentor voltages can provide you withmolecular ion information to confirmmass and fragmentation information todetermine structure
And multisignal data acquired togethercan be linked for easier processing
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Speed peak identification
Automatic alignment of UV and mass-spectral data allows rapid visualizationand peak-to-peak data comparisons.Each peak in the MS and UV chro-matograms is integrated and labeled witha retention time. In mass spectra, majorions are automatically labeled with massinformation.
Speed up data processing for greater productivity
Peak purity software with a multi-component peak in red. The softwaredraws the extracted ion chromatograms below to identify components.
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Powerful, easy-to-use software speeds processing of qualitative andquantitative data. UV and mass-spectral data can be processedtogether using the same software tools. Data processing can be automated and included as part of a method.
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Identify coeluting compounds quickly
Peak purity software uses UV data ormass-spectral data or both to identifychromatographic peaks with incom-pletely-resolved compounds. This isuseful for developing separation methodsor for choosing quantitation ions withoutfurther refinement of the separation.
Toggle buttons make it possible to quicklymove from one impure peak to the nextacross a chromatogram
The peak purity report displays the reten-tion time, number of possible peakcomponents, and characteristic ions foreach peak in a chromatogram
Visualize complex data rapidly
Isoabundance plots of mass-spectraldata allow rapid visualization of complexdata. This feature is useful for detectingpatterns of specific functional groups.
Identify compounds fast
Optional NIST mass-spectral librarysearching quickly identifies compoundsby matching MS spectra with user-createdlibrary spectra. You can combine UV andmass-spectral searching and reporting.The software is optimized for both ESIand APCI data.
Quickly identify com-pounds with optionallibrary search software.
Batch review software helps you evaluate and, if necessary, reprocessthe results for large batches of samples.
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Speed quantitation through automation
Instrument control, data analysis, andreporting can be automated and includedas part of your method.
You can quantitate using both UV and MSsignals in a single calibration table
Quantitation methods can include peakarea or peak height, external standards(ESTD), internal standards (ISTD), peakratios, and multi-point calibration curves
Batch review software provides rapid,interactive user evaluation of results forlarge batches of samples. You can quicklyreview a set of calibration compounds,controls, and samples; and, only if neces-sary, manually reintegrate peaks andrecalculate the calibration curves andsample results
Automated report options are available.Or the drag-and-drop report builder pro-vides an easy way to customize reports
System-wide validation
Because the LC/MSD is a member of the Agilent 1100 Series family of LC andMS modules and systems, you get a com-plete system approach to installationqualification (IQ) and operational qualifi-cation/performance verification (OQ/PV).
The Declaration of System Validationqualifies the Agilent LC/MSD designaccording to documented procedures
IQ and OQ/PV software and services qualify system installation and operation.The ChemStation software includes onlinestandard operating procedures (SOPs) for performing OQ/PV, maintenance, and repairs
The Declaration of Conformity for per-formance verifies that the LC/MSD met specifications prior to its shipmentfrom Agilent
Increase uptime with validation tools, diagnostics, and support
OQ/PV softwarehelps assessongoing systemperformancequickly.
The Agilent 1100 Series LC/MSD includes an array of integrated valida-tion tools to help you meet the most demanding regulatoryrequirements. High reliability, easy-to-use diagnostics, and supportservices increase instrument uptime.
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Maximize uptime
Built-in diagnostics and remote accessmaximize uptime and make maintenanceeasier.
Early maintenance feedback (EMF) software tracks operating time and alerts you when components are due for maintenance
Built-in diagnostic software makes system troubleshooting easy
If maintenance is required, multimediatools, including digital videos, show youhow to do it
Remote access enables remote operationand troubleshooting
Enjoy a range of support services
Agilent offers a broad range of supportservices designed to improve instrumentuptime and to help you comply with regu-latory requirements.
Industry-specific service bundles aredesigned to meet the needs of pharma-ceutical, hydrocarbon processing,chemical, and environmental laboratories
ISO-registered training courses are avail-able at selected locations world wide.On-site courses can be tailored to meetspecial needs
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ChemStationSecurity Packsoftware supports21 CFR Part 11compliance
Software, test files, and methods help thesystem manager assess the functionalityof the system
For data integrity and traceability, thesoftware saves instrument conditions,together with raw data, in checksum-protected binary files
Two password-protected levels of systemaccess ensure operators have only theaccess they need
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Capillary electrophoresis with tandemUV and MS detection
The LC/MSD can be directly coupled to theAgilent 3D CE system to assist with theanalysis of complex mixtures. An optionalcapillary sprayer optimizes performancefor CE/MS analyses.
Combinatorial libraries solution
This molecular-weight confirmationsolution is for the rapid molecular-weight confirmation of combinatoriallibraries—from sample analysis toreporting. Using this system, you canrapidly identify the products of combinato-rial syntheses and estimate their purities.The products can then be purified asnecessary before screening.
Mass-based fraction collection lets youmonitor specific masses and their adductsto trigger fraction collection, thereby col-lecting the compounds of interest.
Complete analytical solutions
Agilent’s 1100 Series LC/MSD Trap system for MS/MS and MSn analyses.
Agilent’s capillary electrophoresis (CE) system with tandem UV and MS detection.
Agilent offers complete analytical solutions with fully-integratedhardware and software. All components are supplied and supported by Agilent.
Agilent’s molecular-weight confirmationsystem for combinatorial libraries.
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MS/MS and MSn solutions
The Agilent 1100 Series LC/MSD Trapsystem provides the multiple stages ofMS data required to solve challengingresearch questions faster and with moreconfidence.
Multiple stages of MS deliver the informa-tion needed to unambiguously characterizecompounds and elucidate structures.
MS/MS provides greater specificity andsensitivity and therefore faster, moreaccurate quantitation—even when chro-matographic peaks are not completelyresolved or sample matrices are complex.
Protein and peptide analysis solutions
Optional software tools make the analysisof proteins and peptides easier.
Protein and peptide analysis softwareeliminates the tedious manual calcula-tions required to match the masses ofpeptide digest fragments to those pre-dicted by digest conditions
Deconvolution software automaticallydeconvolutes mass-spectral data frommultiply-charged ions and assigns molec-ular weights to the mass peaks
Protein database search software searchesprotein databases against mass-spectralinformation generated by peptide maps
High-performance design features
Orthogonal-flow sprayer with pneumaticnebulizer – enhances sensitivity over awide range of LC conditions and reducesmaintenance requirements
High-capacity solvent drying system withuser control of gas flow rate and gastemperature – reduces chemical noiseand permits the analysis of thermally-sensitive compounds
Dielectric platinum-plated capillary – permits independent optimization ofspray chamber and ion optics
User-adjustable in-source fragmentation
Air-cooled vacuum system with dual tur-bomolecular pumps – eliminates theneed for cooling water
Fraction collection capability – can moni-tor and trigger the collection of fractionsbased on mass, an analog signal input, or time. For mass-based determination,up to 16 m/z values can be monitored.Requires optional I/O Accessory Module.
Specifications
Mass rangeVL – m/z 50 - 1500SL – m/z 50 - 3000
Mass accuracy± 0.13 u within the calibrated mass rangein scan mode
Mass axis stability± 0.13 u over 8 hours
SIM sensitivity
• API-electrospray at 400 µl/min or APCI at 1000 µl/min
• Selected ion monitoring of m/z 609.3
• Positive ionization
Quantity Signal-to-Noise Ratio
VL 10 pg 50:1 RMSreserpine (10:1 peak-to-peak)
SL 1 pg 50:1 RMSreserpine (10:1 peak-to-peak)
Scan sensitivity
• API-electrospray at 400 µl/min orAPCI at 1000 µl/min
• Scan range m/z 100 - 650
• Extracted ion at m/z 609.3
• Positive ionization
Quantity Signal-to-Noise Ratio
SL 50 pg 50:1 RMSreserpine (10:1 peak-to-peak)
Multiple signal acquisition (SL model only)Ability to cycle through four differentacquisition modes on a scan-by-scanbasis within a single run.
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For more information
For more information about the Agilent1100 Series LC/MSD, call toll free: 1-800-227-9770 (in the U.S.) or 1-800-341-2477 (in Canada).
In other countries, please call your localAgilent Technologies analytical salesoffice or authorized Agilent Technologiesdistributor.
You can also visit our site on the WorldWide Web at:
http://www.agilent.com/chem
The Agilent 1100 Series LC/MSD hasbeen designed and manufactured undera quality system that has been registeredto ISO 9001.
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Information, descriptions, and specifications in thispublication are subject to change without notice.
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