Agenda For 2018 Northwest Tree Improvement Cooperative Annual Meeting

Date: October 16, 2018 Time: 9:00 AM

Location: Large conference room (downstairs), OSU North Willamette Research and Extension Center, 15210 NE Miley Road, Aurora, OR 97002-9543.

Item Moderated / Presented by Start Time

Coffee and refreshments (provided) 8:30

Call to order, welcome, participants introduce themselves Josh Misenar (NWTIC Chair) 9:00

2017-18 NWTIC activities K. Jayawickrama (NWTIC) 9:10

Update on Modeling Genetic Gains to Rotation Using Molalla Realized Gains Data

Sukhyun Joo (OSU) 9:30

Progress on analyzing data from Douglas-fir SNP chip and implementing Genomic Selection in Douglas-fir

Glenn Howe (OSU-PNWTIRC) 10:15

Short break 10:45

Analysis of age-13 data from Grays Harbor Gain Trial Terrance Ye 11:00

Use of remote sensing (geo-referenced photography) for tree measurement: example from Grays Harbor

Lauren Magalska (Port Blakely) and Jenny Knoth (Green Crow)

11:45

LUNCH (provided) 12:15

Incorporating genetic gain into growth and yield: report on IEG-40 meeting Keith J 12:45

Modified fencing installations on 3rd cycle North Coast tests Fred Pfund and Keith 1:05

Contractors and other resources for tree breeding and testing Josh Misenar 1:10

BUDGET

2018 Budget Account Keith J. 1:20

2019 Budget Keith J. 1:40

Discussion and vote Josh Misenar 2:00

Election of 3 Operations Committee members Josh Misenar 2:30

Date for 2019 annual meeting (October 29) Keith J. 2:45

Report on CAMCORE meeting (Colombia) Keith J. 2:47

Adjourn Josh Misenar 3:15

There is no cost for attending this meeting or for parking at NWREC.

Please confirm attendance at https://doodle.com/poll/wytz8ans3f3wgzxk

https://doodle.com/poll/wytz8ans3f3wgzxk

2017-18: Year In Review for NWTIC

Keith JS Jayawickrama2018 NWTIC Annual Meeting

Mission of the Northwest Tree Improvement Cooperative

• Oversee cooperative breeding of Douglas-fir, western hemlock, and other species of the coastal forests of the Pacific Northwest

• Guide technical aspects of implementing these tree improvement programs

• Analyze and interpret genetic test data

• Store test data and breeding records

• Provide expertise and training in tree breeding

NWTIC Membership Growth and Recruitment

New or returning members for 2018

Potential members for 2019

Redwood growers in NW California (meeting on November 6)

California

Keith met with\contacted with four other potential members

Data Analyses

Completed Analyses

ROSETIC Roseburg High age-7 data (growth, form, budbreak) D-fir

Second-cycle . Heritabilities, site-to-site correlations, predicted gains.

ROSETIC Roseburg Low + Umpqua Phase I and II age-7 data (growth, form, budbreak) D-fir

Second-cycle . Heritabilities, site-to-site correlations, predicted gains. Investigate consistency of cross tested genotypes across Roseburg Low and Umpqua zones (will we need separate Roseburg Low and Umpqua breeding zones going into 3rd cycle)

WACTIC Phase I Age12 data D-fir Second

Grays Harbor Genetic Gain trial age-9/13 data -predicted gains of individual trees D-fir

Detailed analysis of growth, form and wood properties. Growth modeling projection to rotation age. Study of competition effects. Preparing for publication.

WA Coast AV data D-fir Second-cycle. Heritabilities, predicted gains.

WA Coast (5 sites) age-12 data (growth, form) D-fir Second. Heritabilities, site-to-site correlations, predicted gains. Merge in budbreak and other previous results.

ROSETIC Umpua Phase I and II age-10/13 data (growth, form) D-fir

Second. Heritabilities, site-to-site correlations, predicted gains. Merge in budbreak results.

Age-7 data from SCC Phase I D-fir Compare gains from the unreplicated block-plot tests, with gains from randomized single-tree plot tests

NARA\NWTIC\PNWTIRC Genomic Selection projectD-fir Collaborate with PNWTIRC analyzing associations between SNP marker and growth data

This Dormant Season

Joint Genomic Selection Project

Information Management

Database (Now)

■ Size: over 22 gigabytes

■ Records: over 19 million– On average the database

gets about 100,000 records added per year

■ Tables: 31

Database (Past)

• Size: over 19 gigabytes • Records: over 17 million

• On average the database had about 100,000 records added per year

• Tables: 152

Data Process (Now)■ Match IDs

■ Create 1-3 files

■ Load data

Data Process (Past)

• Match IDs• Create file• Create new columns in the

database (if necessary)• Load data • Change view to include new

columns for Progeny and Metadata tables

• Reconnect link to Access database

Process (Original File)

Process (Match progeny ID, create measurement and special columns tables)

Process (load files into the database)

Process (Website: nwtic.forestry.oregonstate.edu)

Website (After data are loaded)

■ Pedigree view

■ Pedigree filtered by Cross

■ Pedigree filtered by Forward Selection

■ Parent locations

■ Parent Gains

■ Cross Gains

Website (After Data load)

■ Progeny Gains filtered by Sites

■ Progeny Gains

■ Progeny filtered by sites

■ Progeny

■ Sites

■ Members

Database Improvement Over the Past Year

■ Generated GID (parent, cross) gains table■ Reformatted all previous parental (backward selection) gains

and loaded to the database■ Generated progeny gains table ■ Reformatted all previous progeny gains and loaded to the

database■ Reformatted all the Gain Trial Sites and loaded to the

database■ 99 percent of the data have been reformatted and reloaded

to the current database format

Website Improvement over the last year■ Programmed functions to retrieve

– Pedigree information, which allows filters of crossIDs and forward selection IDs

– Parent locations specific to program selected– Parental (backward selection) gains– Cross gains– Progeny gains filtered by site– Progeny gains

Data management past 12 months

■ Retrieved data on request

■ Assigned crossIDs for North Coast– crossIDs for CASTIC– crossIDs for MEDTIC

■ Loaded ROSETIC Roseburg High age-7 progeny measurements– ROSETIC Roseburg Low age-7 progeny measurements– WACTIC age-12- Progeny measurements– Grays Harbor gain trial age-13 measurement data– Progeny and gain trial data for South Central Coast– Acoustic velocity data for Washington Coast

■ Generated new progeny and gain trial sites for South Central Coast

Realized Genetic Gain Trials

■ Grays Harbor– Age-13 data collected on four sites (DNR, Port

Blakely, American Forest Management, Green Diamond)

– Includes incidences of forking, ramicorn branching and sinuosity

– Height on all measure trees (SMC measures a subset in each plot)

– Terrance will present results at this meeting■ Molalla

– Keith and Terrance continue to work with Sukhyun Joo (PhD student in the COF) with emphasis on modeling genetic gain to rotation

– Sukhyun will present results at meeting

2nd-cycle Douglas-fir and western hemlock

Genetic Gain Verification Trials

2017 plantations

2018 plantations

2017 plantations

2nd-Cycle and 3rd-Cycle Work and Cooperator

Visits

■ Attended 15 co-op meetings/conference calls■ 9 Field visits ■ 15 sites visited■ 9 visits with cooperators and prospective

cooperators■ Very successful crossing year for 2nd cycle

MEDTIC ■ Frequent consultations

3rd-cycle breeding and testing■ Fine-tuning breeding strategy■ Subline construction■ Stimulation and crossing plans■ North Coast Phase I sowing■ Supporting North Coast Phase I site installation■ CASTIC Medium-elevation sowing coming up

Investigating browse non-preferred

western red cedar

High terpene seedlings, high-terpene cuttings, and controls, planted on 9 demonstration plots in PNW

• 1,100 high terpene cuttings

• 2,600 high terpene seedlings

• 1,100 each of four controls unselected for terpenes

• Total of 7,800 seedlings \ cuttings planted

Staying involved in advances in the field/ representing NWTIC

– NC State Tree Improvement Co-op meeting– IUFRO meeting on breeding for disease resistance– IEG meeting on Genetics and Growth and Yield– CAMCORE meeting

Updated results on simulation of Molalla realized gain trials using CIPSANON

1Department of Forest Engineering, Resources and Management, Oregon State University2Department of Forest Ecosystems and Society, Oregon State University

Sukhyun Joo1, Doug Maguire1, Keith Jayawickrama2, and Terrance Ye2

October 16, 2018

1

Realized gain trials• Northwest Tree Improvement Cooperative (NWTIC)• USDA Forest Service Pacific Northwest Research

Station

• Parents from first-generation progeny tests

• Three genetic levels• Elite• Intermediate• Woods-run (control)

• Elite & Intermediate• Single pair matings of 20 parents

• Woods-run (control)• Random selection of 50 trees

• Established in 1997

2

Experimental Design

Elite

Intermediate

Woods-run

Elite

Intermediate

Woods-run

3.6 × 3.6 m (12 × 12-ft)

1.8 × 1.8 m (6 × 6-ft)

• Split plot design• Whole-plot: planting

density• Sub-plot: genetic level

• 10 families/subplot• 100 trees/subplot• Six replicates at each site• 3600 trees/site

3

Objective

• To predict volume production to rotation age in the realized gain trials with CIPSANON (growth model)

4

(As collaboration between NWTIC and CIPS)

CIPS & NWTIC

CIPS• Director: Doug Maguire

• Simulate improved trees• Yield gains at rotation age• Develop growth equations for

improved Douglas-fir

NWTIC• Director: Keith Jayawickrama

• Measurement data• Predicted & realized genetic gains• Interaction of genetics and

silvicultural practices

5

Simulation study

• Realized gain trials• Colton• Estacada• Silver Falls• Mill City• Molalla• 12 x 12-ft• Elite, Intermediate, Woods-run

• Simulation from age 20, 21, 23, 24

6

Simulation of realized gain trials

• Simulate stands• CIPSANON (beta ver. 4.0.0)• CIPSR R-package beta version (beta ver. 4.0.0)• PNW Variant• Assuming genetic gains were incorporated into site index

• No genetic gain multipliers• Elite, Intermediate, Woods-run• 12 x 12-ft• Initial age 20, 21, 23, 24 (most recent measurements)• Simulation to age 60

7

Results from last year

8

• Using most recent measurements• ORGANON -> CIPSANON• No genetic multipliers but with different site

index

Results – stand volume

12 x 12-ft

• Close to operational planting

9

Results – stand volume

• Percent and absolute stand volume gain over woods-run

• Different site index by plot

• 12 x 12-ft

• Stand volume (ft3/ac)

• Genetic gain at age 13/15

• Elite = 13.7%• Intermediate =

17.3% 10

0

5000

10000

15000

25 30 35 40 45 50 55 60Stand age (yrs)

CFV

ft3

ac

EliteIntermediateWoods-run

10.1%

7.3%

8.7%

6.4%

7.7%

5.8%

7.0%

5.3%

6.4%

4.9%

5.9%

4.5%

5.6%

4.3%

5.2%

4.0%

243

176

346

255

434

324

508

383

571

434

624

477

670

514

709

547

Why is intermediate performing better than Elite?• ANOVA vs. BLUP

• In 1990s, used ANOVA method for selections

• Now using BLUP

• Gain over woods-run at age 15 in progeny test using BLUP generated by Terrance Ye

• Sort largest to smallest gain in HT, DBH, Volume by family

• Elite• Intermediate

• The best performing family was included in the Intermediate

11

Ranking HT gain (%)DBH

gain (%)Volumegain (%)

1 16.70 22.20 58.372 14.15 19.23 54.583 13.74 16.11 41.984 13.65 14.79 40.165 11.59 13.01 37.956 9.71 8.83 22.567 7.22 8.63 21.178 6.24 8.33 15.209 5.45 7.33 13.98

10 5.13 7.12 11.4211 5.12 4.69 10.8812 3.14 3.68 8.6513 3.12 3.20 8.2414 2.95 1.95 3.9515 2.93 0.76 1.8916 2.24 0.53 -0.5017 1.09 -0.25 -1.3618 -1.23 -0.61 -3.1519 -4.64 -1.82 -6.8120 -10.32 -10.27 -19.68

Why is intermediate performing better than Elite? (cont’d)

12

• Only in 12-ft spacing

• In 6-ft, Elite is performing better• From last year’s results

Results – board foot volume

13

• % and absolute BF volume (bf/ac) gain over woods-run

• Log length = 32’ • Minimum log length = 8’ • Top diameter = 6 in• Stump height = 0.5’ • Trim allowance = 8 in

• Different site index by plot

• 12 x 12-ft• BF volume (bf/ac)

Conclusions – stand volume

14

• In 12 x 12-ft, stand volume gain at age 25 • Intermediate = 10.1% • Elite = 7.3%

• In 12 x 12-ft, stand volume gain at age 50• Intermediate = 5.9% • Elite = 4.5%

→ Elite: 61.6 % volume gain remained at age 50 compared to age 25 → Intermediate: 58.4 % volume gain remained at age 50 compared to age 25

Remained gain at age 50 %

=VOL Gainage 50VOL Gainage 25

× 100

• In 12 x 12-ft, board foot (BF) volume gain at age 25 • Intermediate = 13.0% • Elite = 8.6%

• In 12 x 12-ft, board foot (BF) volume gain at age 50• Intermediate = 6.2% • Elite = 4.6%

→ Elite: 53.5% BF volume gain remained at age 50 compared to age 25 → Intermediate: 47.7 % BF volume gain remained at age 50 compared to age 25

Conclusions – board foot volume

15

Remained gain at age 50 %

=BF Gainage 50BF Gainage 20

× 100

00_Agenda for 2018 Annual NWTIC meeting_v1Agenda For 2018 Northwest Tree Improvement Cooperative Annual Meeting

01_2017-18 NWTIC highlights2017-18: Year In Review for NWTICSlide Number 2Slide Number 3NWTIC Membership Growth and RecruitmentSlide Number 5Data AnalysesSlide Number 7Joint Genomic Selection ProjectInformation ManagementDatabase (Now)Data Process (Now)Process (Original File)Process (Match progeny ID, create measurement and special columns tables)Process (load files into the database)Process (Website: nwtic.forestry.oregonstate.edu)Website (After data are loaded)Website (After Data load)Database Improvement Over the Past YearWebsite Improvement over the last yearData management past 12 monthsRealized Genetic Gain TrialsSlide Number 222nd-cycle Douglas-fir and western hemlock Genetic Gain Verification TrialsSlide Number 24Slide Number 25Slide Number 262nd-Cycle and 3rd-Cycle Work and Cooperator VisitsSlide Number 283rd-cycle breeding and testingInvestigating browse non-preferred western red cedarSlide Number 31Slide Number 32Staying involved in advances in the field/ representing NWTIC

02_Joo_Molalla Gain Trial_2018 NWTIC meetingUpdated results on simulation of Molalla realized gain trials using CIPSANONSlide Number 2Slide Number 3Slide Number 4Slide Number 5Slide Number 6Slide Number 7Slide Number 8Slide Number 9Slide Number 10Slide Number 11Slide Number 12Slide Number 13Slide Number 14Slide Number 15