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Transcript of Agela
Agela Technologies — A Global Supplier for Chromatography Solutions
2008 Message From Agela Technologies
As Agela technologies is poised to enter its fifth year with confidence and pride in
its innovative separation, purification, and sample preparation products, we would
like to thank our many loyal customers for your continuous support and trust. With
your support and our effort in delivering the highest quality products to you, our
company has grown remarkably. This has allowed us to expand our research and
development effort, and thus introduce more innovative products to better service
your application needs.
In 2007, we had tremendous accomplishments: We cataloged over one thousand
different products. Our manufacturing and R&D operation was certified in compli-
ance with ISO 9001 and passed many quality audits by customers and distributors,
including VWR. As a global wide company, we not only have our own international
sales force but have also formed a marketing alliance with VWR international. This
will allow us to reach higher goals for 2008 and to provide our customers with even
better quality products and faster service.
Provide products with our INNOVATIVE technologies at the best PERFOR
MANCE to COST ratio.
Deliver products with guaranteed quality.
Provide global support with quick responses
Our 2008 mission statement and commitment:
How to Place Orders
Terms and ConditionsPLEASE READ THESE TERMS BEFORE ORDERING. IF YOU HAVE ANY QUESTIONS, PLEASE DO NOT HESITATE TO
CONTACT US AND OUR STAFF WILL BE GLAD TO ASSIST YOU.
Our office is open from 9:00 am to 6:00 pm Eastern
Standard Time, Monday through Friday.
To place an order or receive a quote, you may choose from
the following contacts:
Mail Address: Agela Technologies Inc.
625 DAWSON DRIVE, Newark, DE19713
Phone: (302) 438-8798
Fax: (302) 235-1833
Email: [email protected]
On-line: www.agela.com
Please include the following information with your order or
request: Account number (if you have one), purchase order
number, contact name, organization name, shipping and
billing address, telephone number, fax number or email
address, product number, brief description and quantity,
method of payment, preferred method of delivery. A written
confirmation will be sent to you by email or fax. We accept
business checks, wire transfers, and major credit cards as
methos of payment.
Checks:
Please make checks payable to:
Agela Technologies Inc. and send to:
Agela Technologies Inc.
625 DAWSON DRIVE, Newark, DE19713
Wire Transfer:
Please contact us by phone, fax or email for account
information.
Credit Cards:
Please include the card type and number, expiration date,
and card holder name. Due to security concerns, please
do not email the information. Please call or send a fax to
provide your credit card information.
Acceptance and Availability
All orders placed are subject to the agreement of Agela
Technologies Inc. The catalogue does not constitute an
engagement of the company to sell all listed products. You
are guaranteed to be notified at the time of ordering if the
ordered items are in back-order or discontinued.
Price and Payment
The prices are in effect at the time of printing. Agela
Technologies reserves the right to change the prices without
notice, though we do our best to provide our customers with
advance notice. The prices quoted at the time of ordering
will be guaranteed. The general payment term is net 30
days, F.O.B., Newark, Delaware,USA. However we reserve
the right to ask for prepayment if customers’ account
information is not satisfactory. A 1.5% per month service
charge will be added to delinquent accounts. If a purchase
order is less than $1000.00, a $50.00 extra charge will be
added to the invoice.
Changes
Agela Technologies reserves the right to change product
specifications, quantities, designs or prices without prior
notice and without liability for such changes.
Shipping Policy
The standard shipping method is 2 day FedEx within the
United States and Canada. We will try to accommodate
requests for other shipping methods if they are available. All
shipping and handling charges will be billed separately.
Should you receive damaged goods, it is imperative that
you notify us immediately and save all packing materials for
inspection by the carrier.
Application
All products in this catalog should be used for laboratory or
manufacturing use only. They are not intended for direct
medicinal or food use. Agela Technologies assumes no
liability for any misuse of the products.
Returns
Agela Technologies tries to accommodate all requests for
returns of unused goods. However,return of some items
may be restricted by the original manufacturers. Please
contact us for return authorization before returning any
items. A restocking charge may be applied to certain
products.
Warranty
All Agela Technologies products are warranted to be free of
defects in materials and workmanship. They are not
warranted for any other particular purpose. Agela Technolo-
gies shall not under any circumstance be liable for any
incidental, consequential or compensatory damage in
conjunction with its products. The maximum liability shall
not exceed the invoice price of the product.
HPLC Accessories and Supplies
SPE Accessories and Supplies
GC Accessories and Supplies
Filtration Products
Vials and Caps
Cleanert SPE Columns,Wellplates and Media
The Development of SPE Method
Applications
Cross-reference Table
Introduction
HPLC Columns by Family
Selected Solutions for Popular Applications
Ordering Information by the Type of Stationary Phases
HPLC Column Selection Guide
Applications
Appendix
002
002
033
044
054
058
075
HPLC Columns and Media
SPE Columns and Wellplates
Purification Products
GC Columns
Accessories and Supplies146
153
157
162
166
127
078
103
106
114
Preparative HPLC Columns
Flash Chromatography Media and Columns
Flash Chromatography System
116
120
125
HPLC Columnsand Media
Introduction
HPLC Columns by Family
Unisol Technology and Column Products–NEW
Optimix Technology Column Products–NEW
Durashell Technology and Column Products –NEW
Venusil Family of HPLC Columns
Promosil HPLC Columns
Other Manufacturers’ Brands
Selected Solutions for Popular Applications
Solutions for Highly Water Soluble Compounds
Solutions for Low pH and High pH Applications
Solutions for LC-MS
Solutions for Fast Analysis
Solutions for Bio-molecules
Solutions for Preparative HPLC
Solutions for SFC
Ordering Information by the Type of Stationary Phases
Reverse Phase Columns
Normal Phase Columns
Ion-exchange Columns
Mixed Phase Columns
Size Exclusion Columns (Silica Based)
Polymer Phase Columns
HPLC Column Selection Guide
Applications
Appendix
002
002
002
011
015
017
031
032
033
033
039
040
042
043
043
043
044
044
047
049
050
051
051
054
058
075
HP
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AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
As one of the emerging leaders in chromatography technology, Agela Technologies proudly presents five new HPLC columns developed
using our latest cutting-edge technologies. These products exhibit superior performance and are designed to meet the ever-increasing
demands of our customers.
Agela’ s unique sol-gel process technology generates a uniform surface that produces a universal column applicable to many HPLC applications.
Peak tailing adversely affects chromatographic efficiency and reduces the resulting accuracy and precision of the data. Peak tailing and
insufficient retention of polar compounds are among the toughest problems for silica based reverse phase columns. High acidity of the
surface silanols, non-uniformity of the surface, and non-uniformity of the bonding process are the three major causes of peak tailing.
High purity synthetic silica is typically obtained by hydrolyzing silicic acid esters followed by high temperature sinteration. The sintered silica
is further leached with dilute acid to enrich silanol on the surface. The process does not generate a uniform, silanol covered surface. It will
create some isolated silanol groups with very strong acidity or some “un-leached islands” during the leaching process. Furthermore, the high
temperature procedure may cause formation of micro-crystallization domains on the amorphous silica, further contributing to the non-
uniformity of the surface.
Unisol process technology is specifically designed to solve these problems. In our patented Unisol process, gas phase tetramethoxy silane is
adsorbed evenly on the silica surface. It is subsequently hydrolyzed by a controlled amount of moisture. A fresh layer of silanols is then
formed by a mild heating process. This process produces a surface with lower acidity and high uniformity, which is further bonded with a high
purity C18 silane, and then di-functional end-capped. The overall process yields an excellent HPLC stationary phase with a very smooth
surface that is friendly to basic compounds (minimal ionic interactions) and polar compounds (provides significantly increased retention).
Major benefits of the Unisol Technology for reverse phase and normal phase chromatography:
1.Unisol C18 - A type of column that is a true universal reverse phase column for the separation of acidic, basic, and neutral molecules. It
has superior performance to any conventional C18 phases in almost every aspect.
2.Unisol Amide - A unique phase that is very different from other stationary phases in its separation mechanism. It can be used as a RP or
a NP column. It has superior performance than any of the other HILLIC columns on the market. It has superior retention for polar com-
pounds when operated as a HILIC column.
3.Optimix columns – A class of columns that provide optimized selectivity and performance for HPLC separations. It has balanced hydro-
philicity and hydrophobicity: extended retention for polar compounds; non-excessive retention for strong hydrophobic compounds.
4.Durashell columns - A class of column that can be used in a wide pH range of 1-12.
5.Venusil ASB columns – A class of columns that are excellent for low pH and LC-MS applications.
●Greatly improved peak shape for basic compounds
●100% water compatibility
●Significantly increased retention for highly water soluble compounds and balanced hydrophobicity in the reverse phase mode
●Elimination of excessive retention for polar compounds in the normal phase mode ( “hot spots” )
Unisol Technology and Column Products NEW in HPLC !
HPLC Columns by Family
Introduction
HP
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AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
Balanced Retention for Hydrophilic and Hydrophobic Compounds
Mobile Phase:35% 20 mM KH2PO
4 pH=7.0, 65% Methanol
Temperature: 23
Flow: 1mL/min
Detection: UV 254 nm
Sample: Uracil
Propranolol hydrochloride
Butyl Phthalate
Dimethyl Phthalate,
Naphthalene
Acenaphthene
Amitriptyline
Extremely Low Metal Effects
Mobile Phase: A: Water, B: Methanol; A:B = 65:35
Flow Rate: 1 mL/min
Temperature: 25
Detection: UV 230 nm
Sample: 2,3-Dihydroxynaphthalene 2,7-Dihydroxynaphthalene
Unisol C18 5μm, 4.6mm×150mm Brand W, AQ Column 5μm, 4.6mm×150mm
Brand H, C18 Column 5μm, 4.6mm×150mm Brand Z,C18 Column 5μm, 4.6mm×150mm
min0 1 2 3 4 5 6 7
mAU
0
10
20
30
40
VWD1 A, Waveleng th=230 nm (LPF\07091702.D)
3.0
08
4.0
84
TF=1.31
min0 1 2 3 4 5 6
mAU
0
10
20
30
40
50
VWD1 A, Wavelength=230 nm (LPF\07090605.D)
Are
a: 2
53.2
88
2.2
75
Are
a: 4
00.3
99
2.8
89
TF=1.40
min0 10 20 30 40 50 60
mAU
0
100
200
300
400
500 VWD1 A, Wavelength=254 nm (HLX\08021110.D)
1.6
41
3.2
17
6.4
97
8.2
33
14
.13
7
31
.06
1
48
.84
2Unisol C18 ,5μm, 4.6*150mm
Part No.: UO951505-0
min0 10 20 30 40 50 60
mAU
0
100
200
300
400
VWD1 A, Wavelength=254 nm (MSW\08013140.D) 1.6
50
3.5
83
7.5
30
9.1
30
16
.62
1
37
.59
9
58
.32
2
Brand W C18, 5μm, 4.6*150mm
min0 10 20 30 40 50 60
mAU
0
100
200
300
400
VWD1 A, Wavelength=254 nm (MSW\08020100.D) 1.9
09
3.0
06
5.6
16
6.7
32 7
.42
1
14
.01
2
60
.33
9
Brand Z C18, 5μm, 4.6*150mm
min0 1 2 3 4 5 6 7
mAU
0
5
10
15
20
25
30
35
40
VWD1 A, Waveleng th=230 nm (LPF\07091208.D)
2.4
50
3.4
71
min0 2 4 6 8 10
mAU
5
10
15
20
25
VWD1 A, Wavelength=230 nm (LPF\07091202.D)
3.9
44
Are
a: 1
76.6
86
5.9
34
TF=1.69 TF=2.88
HP
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Unisol C18
A Slightly Polar, 100% Water Compatible and Universal Reverse Phase
A unique and universal C18 HPLC phase made by our patented Unisol Technology. Unprecedented separation performance for compounds
with a wide range of properties from hydrophilic to hydrophobic: polar, semi-polar and non-polar compounds.
Characteristics: Metal Impurity < 10 ppm; Pore Size 100 Å; Specific Surface Area 410 m2/g; Carbon Loading 17%; Single end-cap; Avail-
able Particle Size: 2.5 μm, 3μm, 5μm, 10μm
Consider this column as the first option for your HPLC method development
●One of the most universal reverse phase columns
●Great inertness and efficiency for basic compounds
●Enhanced retention of hydrophilic compounds
●100% aqueous compatibility
●Robust and reproducible performance
●Wide pH range (1.5-9.0)
●Low bleed and high sensitivity for LC-MS
Sample: uracil, toluene, doxepin, amitriptyline
Column: 4.6 x 150mm, 5 μm
Mobile phase:10 mM sodium phosphate ( pH =7 ) in 60% ACN.
Temperature: 30 ; Flow rate: 1 mL /min.
The plate numbers in regular font are for toluene, the plate
numbers in Italic are for amitriptyline
Great Inertness and Efficiency for Basic Compounds
Best ValueGuaranteed Product QualityInnovation to Benefit Customers
YMC AQ Column4.6×250mm,5μm
Mobile Phase A: Water (Contain 0.01mM Na
2PO
4 and 0.01mM Citric Acid, pH=4.5 )
B: ACN , A:B =95:5Sample: Cytosine, Uracil, Guanine
Temperature 35Detection 254 nm
Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556
Waters-Atlantis
12071
14695
4010 12083
Unisol C18
Brand K C18
Inertsil C18
Brand A C18
Waters-Xterra C18
1638
14316
13792
9518
15307
4533
12936
6772
Good Retention for Polar Compounds
Unisol C184.6×250mm,5μm
Part No.: UO952505-0
HP
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Sample: uridine in calf blood plasma (diluted 20 times with 0.1%
formic acid)
Injection: 1μl (not be cleaned up with SPE)
Column: Unisol C18 2.1×50mm,5μm
Temperature: 30
Flow Rate: 0.3mL/min
Mobile phase:0.1% formic acid solution (100% aqueous phase)
Run time: 8 min
After 500 injection, wash with methanol /0.1% formic acid solution =
50/50 for 30mins column temperature 40 ; rinse with 95%MeOH for
30mins, column temperature 45
Good Tolerance of Directly Injected Biological Matrix
Best Value
Guaranteed Product Quality
Innovation to Benefit Customers
0 1 2 3 4 5 6 7 min
0 1 2 3 4 5 6 7 min
0 1 2 3 4 5 6 7 min
mAU60
50
40
30
20
10
0
-10
Fresh Colunm
Plates = 3224
mAU60
50
40
30
20
10
0
-10
After column washing and re-equlibrium in
100% water (0.1% formic acid)
Plates = 3045
mAU60
50
40
30
20
10
0
-10
After 500 sample injections
Plates = 2810
Analysis of Quaternary Ammonium Alkaloids from Copitidis with a
Non-Volatile Ion Pair Reagent using Brand Z C18 Column.
Mobile Phase: ACN:0.05 mol/L KH2PO
4 Aq.
(40 60, Contain 0.015 mol/L SDS)
Analysis of Quaternary Ammonium Alkaloids from Copitidis without a
Non-Volatile Ion Pair ReagentUsing Unisol C18 Column .
Mobile Phase : Water + 0.2 TFA :acetonitrile= 75:25
Enable Methods to be Compatible with LC-MS
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
0
0
50
100
150
200
250
5 10 15 20 25 30 35 40
mAU
min
0.00
0.00
0.05
0.10
0.15
0.20
0.25
5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00 45.00 50.00
HP
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AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
Compatibility with 100% Aqueous Mobile Phase
mAU
20
10
0
0 2 4 6 8 10 12 14 16 18 min
0 2 4 6 8 10 12 14 16 18 min
0 2 4 6 8 10 12 14 16 18 min
0 2 4 6 8 10 12 14 16 18 min
0 2 4 6 8 10 12 14 16 18 min
0 2 4 6 8 10 12 14 16 18 min
Unisol C18 , initial
mAU
20
10
0
Unisol C18
after 4 hours
mAU
20
10
0
Luna C18 initial
mAU
200
100
0
Brand K C8 initial
mAU
200
100
0
Luna C18 after 4 hours
mAU
400
200
0
Brand K after 4 hours
Sample: Uridine
Column: 4.6 x 150 mm,5 μm
Mobile phase:100% water
Flow rate: 1mL/min; the flow was stopped for 5 minutes
during the testing period for each column.
Temperature: 30oC
Greater Volume Loading Capacity Than Other Brands
Large Volume Injection (Injection volume =100 μL)
Sample: 4-methoxybenzenesulfon amide,
10 μg/mL in MeOH/Water (1:1)
Column: 4.6 x 150 mm, 5μm
Mobile phase: MeOH/Water
Flow rate: 1mL/min
Temperature: 30 oC
min0 1 2 3 4 5
mAU
0
50
100
150
200
min0 1 2 3 4 5
mAU
0
50
100
150
200
0
50
100
150
200
min0 1 2 3 4 5
mAU
Unisol C18, Plates=9137
Brand A-C18, Plates=2029
Brand Z-C18, Plates=2118
HP
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Best Value
Guaranteed Product Quality
Innovation to Benefit Customers
Stability of Unisol C18 at pH=1.5
Sample: Uracil, Doxepin, Amitriptyline,Toluene.
Column: Unisol C18, 4.6 x 150 mm, 5 μm
Mobile phase: TFA (0.25%, pH 1.5) in 60% acetonitrile
Flow rate: 1 mL/ min.;
Temperature: 40 oC
mAU
40
30
20
10
0
0 1 2 3 4 5 6 7 8 9 min
After 4 hours
0 1 2 3 4 5 6 7 8 9 min
mAU
40
30
20
10
0
After 500 hours
Sample: Uracil, Doxepin, Nortriptyline,Amitriptyline, Trimipramine
Column: Unisol C18, 4.6 x 250 mm,5 μm
Mobile phase: 0.01 M sodium phosphate : ACN = 25 : 75, pH=7.0
Flow rate: 1.3 mL/min.
Temperature: 30oC
mAU
40
35
30
25
20
15
10
5
00 5 10 15 20 25 30 min
2.08
0 2.32
0
4.06
8
6.96
1
9.77
5 14.2
76
Initial
mAU
40
35
30
25
20
15
10
5
00 5 10 15 20 25 30 min
2.04
0 2.31
1
4.33
7
6.09
0
9.96
5 14.2
71
After 150 hours
Perfect Peak Symmetry and Good Stability at Mid-pH for Basic Compounds
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
HP
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AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
Unisol Amide (HILIC)
Unisol Amide stationary phase is made from high purity spherical silica particles bonded with a neutral hydrophilic amide group. It can be
used for normal phase reverse phase, or hydrophilic interaction HPLC. The Unisol Amide (NP) column is a good replacement for NH2 and
Silica columns that are currently marketed as HILIC. Compared with traditional silica and NH2 columns, the Unisol Amide columns have
better reproducibility and column lifetime. It is especially useful for the separation of strong hydrophilic compounds, whether they are acidic,
basic or neutral. It is compatible with aqueous mobile phases in the pH range of 2-8. It has selectivity very different from most of conven-
tional stationary phases
Characteristics: Metal Impurity < 10 ppm; Pore Size 100 Å; Specific Surface Area 410 m2/g; Carbon Loading 6%; Single End-cap; Available
Particle Size: 3μm, 5μm, 10μm
Main features:
●Strong retention of polar compounds in HILIC mode
●Very unique selectivity and good complementary to the conventional reverse phase
●Can be used as reverse phase, normal phase and HILIC phase
●More robust and reproducible performance than silica and amino stationary phase
●100% aqueous and 100% organic solvents compatible
●pH range (2-8)
Separation of Validamycin
Mobile Phase: A: 0.1% TFA in WaterB: Acetonitrile
Gradient: 40% A to 85% A in 30 min
Flow Rate: 1 mL/min
Temperature: 25
Detection: UV 210 nm
Sample: Validamycin
Mobile Phase: A: 0.005M NaH2PO
4pH=7) ; B:MeOHA:B=98:2
Flow Rate: 1 mL/min
Temperature: 25
Detection: UV 210 nm
Sample: Validamycin
OONH
CH2OH
HOH2COH
OH
CH2OH
OHHO
OH
OH
HO OH
Column: Unisol Amide,5μm, 4.6mm×250mm
Part No.: UA952505-0
Column:Brand W ,
AQ Column,5μm, 4.6mm×250mm
Au0.12
Au0.20
0.18
0.16
0.14
0.12
0.10
0.08
0.06
0.04
0.02
0.00
0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50 min
-0.02
0.10
0.08
0.06
0.04
0.02
0.00
-0.02
2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00 18.00 min
HP
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Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
Best Value
Guaranteed Product Quality
Innovation to Benefit Customers
m in0 1 2 3 4 5 6
m AU
0
1 0 0
2 0 0
3 0 0
4 0 0
5 0 0
D AD 1 A , S ig = 28 0 ,4 R e f= 3 6 0 ,1 0 0 (AG E LA\0 7 01 3 0 0 0 .D )
1.9
57
2.3
00
3.3
92
5.5
73
m in0 1 2 3 4 5 6
m A U
0
5 0
1 0 0
1 5 0
2 0 0
2 5 0
D A D 1 A , S ig = 2 8 0 ,4 R e f = 3 6 0 ,1 0 0 ( A G E L A \0 7 0 2 1 1 0 9 .D )
1.6
58
1.7
68
3.5
73
4.6
79
m in0 1 2 3 4 5 6
m A U
0
5 0
1 0 0
1 5 0
2 0 0
D A D 1 A , S ig = 2 8 0 ,4 R e f= 3 6 0 ,1 0 0 (A G E L A \0 7 0 2 1 1 1 4 .D )
3.2
08
3.4
87
3.8
35
4.4
04
Separation of Shikimic Acid
Column: Unisol Amide, 5 μm, 4.6mm×150mm
Part No.: UA951505-0
Mobile Phase:A: 1% Fomic Acid in Water B: Acetonitrile
Gradient: 60% A to 90% A in 20 min
Flow Rate: 1 mL/min
Temperature: 25
Detection: UV 210 nm
Sample: Shikimic Acid
Mobile Phase:A: 0.1% TFA in Water B: 0.1% TFA in Acetonitrile
A: B = 90:10
Flow Rate: 1 mL/min
Temperature: 30
Detection: UV 280 nm
Sample: VB1+VB6+VC+VB2
Water-soluble Vitamins
Unisol Amide 5 μm, 4.6mm×150mm
Part No.: UA951505-0
Brand W Silica Column, 5μm, 4.6×150mm
Brand W, NH2 Column, 5 μm, 4.6×150 mm
HP
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AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
Ordering Information
Surface Area: 410 m2/g, Pore Size: 100Å
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Gaurd cartridge
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Gaurd cartridge
Semi-preparative
Semi-preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Guard cartridge
2.1×30
2.1×50
2.1×100
2.1×150
4.6×50
4.6×100
4.6×150
4.6×250
4.6×10
2.1×30
2.1×50
2.1×100
2.1×150
4.6×50
4.6×100
4.6×150
4.6×10
10×150
10×250
20×50
20×150
20×250
30×100
30×150
30×250
20×20
UO950302-0
UO950502-0
UO951002-0
UO951502-0
UO950505-0
UO951005-0
UO951505-0
UO952505-0
UO950105-0
UO930302-0
UO930502-0
UO931002-0
UO931502-0
UO930505-0
UO931005-0
UO931505-0
UO930105-0
UO951510-0
UO952510-0
UO950520-0
UO951520-0
UO952520-0
UO951030-0
UO951530-0
UO952530-0
UO950220-0
VH950302-0
VH950502-0
VH951002-0
VH951502-0
VH950505-0
VH951005-0
VH951505-0
VH952505-0
VH950105-0
VH930302-0
VH930502-0
VH931002-0
VH931502-0
VH930505-0
VH931005-0
VH931505-0
VH930105-0
VH951510-0
VH952510-0
VH950520-0
VH951520-0
VH952520-0
VH951030-0
VH951530-0
VH952530-0
VH950220-0
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
3
5
5
5
5
5
5
5
5
5
2.1×30
2.1×50
2.1×100
3.0×30
2.5
2.5
2.5
2.5
UO920302-0
UO920502-0
UO921002-0
UO920303-0
3.0×50
3.0×100
4.6×50
4.6×100
2.5
2.5
2.5
2.5
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
3
3
3
3.0×50
3.0×100
3.0×30
3.0×50
3.0×100
3.0×30 5
5
5
Analytical
Analytical
Analytical
UO930303-0
UO930503-0
UO931003-0
UO950303-0
UO950503-0
UO951003-0
UO920503-0
UO921003-0UO920505-0
UO921005-0
VH950303-0
VH950503-0
VH951003-0
VH930303-0
VH930503-0
VH931003-0
Type Dimension (mm) Particle Size(µm) Unisol C18 Unisol Amide(HILIC)
HP
LC C
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011
Conventional silica based HPLC stationary phases are usually bonded with mono-type alkyl chain molecules, e.g., C18, C8, CN, etc. Recent
studies revealed that synergistic effects can be obtained when the bonded surface consists of mixed phases of C18 and a shorter chain (C8,
amide, CN, nitro-phenyl, etc). These new products offer different selectivity, coordinated multi-functionality, as well as a spacer effect
(C18/C8).
It is difficult to produce a uniformly bonded C18 surface on silica due to steric hindrance of the bulky C18 chain. This problem can be easily
overcome when the surface is bonded with mixed chain lengths, C18 and a shorter silane. As a result, there are many advantages:
improved surface uniformity, better penetration of molecules during partitioning due to less steric hindrance, and different selectivity com-
pared to a mono-chain type stationary phase.
As one of the leaders in separation technologies, Agela Technologies has innovatively developed a family of non-ionic, mixed-phase
HPLC/SFC columns.
Consider these columns as options for your HPLC method development
Improved performance from a mixed-phase C18/C8 column compared to mono-chain C18 or C8 columns
●Alternative selectivity
●Improved selectivity for stereo isomers, better differentiation of shapes
●Better peak shape due to uniformity of the bonding.
●Balanced hydrophilicity and hydrophobicity: extended retention for polar compounds; non-excessive retention for strong hydrophobic compounds
Characteristics: Metal Impurity < 10 ppm; Pore Size: 100 Å; Specific Surface Area: 410 m2/g; Carbon Loading: 16%; Double End-cap; Avail-
able Particle Size: 3µm, 5µm, 10µm.
NEW in HPLC !Optimix Technology Column Products
Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556
Best ValueGuaranteed Product QualityInnovation to Benefit Customers
Optimix C18/C8:
Analysis of Vancomycin and its impurity
Vancomycin ImpurityNH
2
H3C
HO
CH3
OHHO
OH
OH
CH3
CH3
CI
CI
OO
OO
OO
O
O
O O
O
O
O
HO
HO
HOOC
H
H H
H HH
3C
NH2
HHHHN
NH
HN
NH
NH
HN
HN
H
OHOH
NH2
H3C
HO
CH3
OHHO
OH
OH
CH3
CH3
CI
OO
OO
OO
O
O
O O
O
O
O
HO
HO
HOOC
H
H H
H HH
3C
NH2
HHHHN
NH
HN
NH
NH
HN
HN
H
OHOH
HP
LC
Colu
mns
012
AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
Column: Optimix C18 /C8,5μm 4.6×250mm
Part No.: OP952505-OC
Mobile Phase:A: 0.7% TEA and 3% Acetic Acid in Water
B: Methanol ;C:THF ;A:B:C(88:10:2)
Flow Rate: 1 mL/min
Temperature: 25
Detection: UV 210 nm
Sample: Vancomycin Impurity
Vancomycin
Impurity
Column: Brand W C18 Column,5μm , 4.6×250mm
Mobile Phase:USP 24
A: 0.2% TEA (pH3.2)-Acetonitrile-THF(92:7:1)
B: 0.2% TEA (pH3.2)-Acetonitrile-THF(70:29:1)
Gradient USP 24
Time(min) 0 12 20 22 23 30
B% 0 0 100 100 0 0
1.0e+2
9.5e+1
9.0e+1
8.5e+1
8.0e+1
7.5e+1
7.0e+1
6.5e+1
6.0e+1
5.5e+1
5.0e+1
4.5e+1
4.0e+1
3.5e+1
3.0e+1
2.5e+1
2.0e+1
1.5e+1
1.0e+1
5.0
5.00 10.00 15.00 20.00 25.00Time
12.52
11.52
Impurity
Vancomycin
1.0e+2
9.5e+1
9.0e+1
8.5e+1
8.0e+1
7.5e+1
7.0e+1
6.5e+1
6.0e+1
5.5e+1
5.0e+1
4.5e+1
4.0e+1
3.5e+1
3.0e+1
2.5e+1
2.0e+1
1.5e+1
1.0e+1
5.0
20.0017.5015.0012.5010.007.50Time
HP
LC C
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Best ValueGuaranteed Product QualityInnovation to Benefit Customers
Optimix C18/Amide
A mixed-phase of C18 and propionic amide provides unique selectivity which allows extremely polar and non-polar compounds to be ana-
lyzed in a single run under isocratic conditions.
Characteristics: Metal Impurity < 10 ppm; Pore Size: 100 Å; Specific Surface Area : 410 m2/g; Carbon Loading : 11%; Single End-cap;
Available Particle Size: 3 µm, 5 µm
Performance Features:
●Unique selectivity
●Balanced retention for extremely polar and non-polar compounds
●Greater retention for polar compounds due to coordinated effects of C18 and amide
●Minimal tailing for basic compounds compared to any other type of columns, due to suppression of silanol effects by the amide group.
Bring extremely polar and non-polar analytes closer to shorten the analysis time under isocratic conditions
Mobile Phase A: Water, B: Methanol
Sample: Uracil, Toluene
Flow rate: 1 mL/min
Detection: UV = 254 nm
t0= 1.52 min
Column: Optimix C18/amide,5μm, 4.6×150mm
Mobile Phase: A : B = 50 : 5
Column: C18, 5μm,4.6×150mm
Mobile Phase: A : B = 60 : 40
Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556
t=2.023 min
t= 15.278 min
t=1.658 min
t=57.216 min
HP
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AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
Ordering Information
Surface Area: 410 m2/g, Pore Size: 100Å
Type Dimension (mm) Particle size(µm) Optimix C18/SCX Optimix C18/SAX Optimix C18/Nitrophenyl
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Guard cartridge
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Guard cartridge
2.1×30
2.1×50
2.1×100
2.1×150
4.6×50
4.6×100
4.6×150
4.6×200
4.6×250
4.6×10
2.1×30
2.1×50
2.1×100
2.1×150
4.6×50
4.6×100
4.6×150
4.6×10
OP950302-SC
OP950502-SC
OP951002-SC
OP951502-SC
OP950505-SC
OP951005-SC
OP951505-SC
OP952005-SC
OP952505-SC
OP950105-SC
OP930302-SC
OP930502-SC
OP931002-SC
OP931502-SC
OP930505-SC
OP931005-SC
OP931505-SC
OP930105-SC
OP950302-SA
OP950502-SA
OP951002-SA
OP951502-SA
OP950505-SA
OP951005-SA
OP951505-SA
OP952005-SA
OP952505-SA
OP950105-SA
OP930302-SA
OP930502-SA
OP931002-SA
OP931502-SA
OP930505-SA
OP931005-SA
OP931505-SA
OP930105-SA
OP950302-NP
OP950502-NP
OP951002-NP
OP951502-NP
OP950505-NP
OP951005-NP
OP951505-NP
OP952005-NP
OP952505-NP
OP950105-NP
OP930302-NP
OP930502-NP
OP931002-NP
OP931502-NP
OP930505-NP
OP931005-NP
OP931505-NP
OP930105-NP
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
3
Type Dimension (mm) Particle size(µm) Optimix C18/C8 Optimix C18/amide Optimix C18/phenyl Optimix C18/CN
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Guard cartridge
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Guard cartridge
2.1×30
2.1×50
2.1×100
2.1×150
4.6×50
4.6×100
4.6×150
4.6×200
4.6×250
4.6×10
2.1×30
2.1×50
2.1×100
2.1×150
4.6×50
4.6×100
4.6×150
4.6×10
OP950302-OC
OP950502-OC
OP951002-OC
OP951502-OC
OP950505-OC
OP951005-OC
OP951505-OC
OP952005-OC
OP952505-OC
OP950105-OC
OP930302-OC
OP930502-OC
OP931002-OC
OP931502-OC
OP930505-OC
OP931005-OC
OP931505-OC
OP930105-OC
OP950302-AM
OP950502-AM
OP951002-AM
OP951502-AM
OP950505-AM
OP951005-AM
OP951505-AM
OP952005-AM
OP952505-AM
OP950105-AM
OP930302-AM
OP930502-AM
OP931002-AM
OP931502-AM
OP930505-AM
OP931005-AM
OP931505-AM
OP930105-AM
OP950302-PH
OP950502-PH
OP951002-PH
OP951502-PH
OP950505-PH
OP951005-PH
OP951505-PH
OP952005-PH
OP952505-PH
OP950105-PH
OP930302-PH
OP930502-PH
OP931002-PH
OP931502-PH
OP930505-PH
OP931005-PH
OP931505-PH
OP930105-PH
OP950302-CN
OP950502-CN
OP951002-CN
OP951502-CN
OP950505-CN
OP951005-CN
OP951505-CN
OP952005-CN
OP952505-CN
OP950105-CN
OP930302-CN
OP930502-CN
OP931002-CN
OP931502-CN
OP930505-CN
OP931005-CN
OP931505-CN
OP930105-CN
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
3
HP
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Best ValueGuaranteed Product QualityInnovation to Benefit Customers
Durashell Technology and Column Products
Agela’ s Durashell column has a pH range of 1-12. Different from the current marketed products that also can be used at high pH,
Durashell’ s stationary phase is made with a dentrimer technology followed by molecular modifications. Agela’ s technology produces a
strong hydrophobic protection layer over the silica surface allowing the columns to be used at extremely high and low pH. This technology
also reduces excessive hydrophobic interactions between the stationary phase and the compounds, and yet maintains great interfacial kinet-
ics for high efficiency.
Characteristics: Metal Impurity < 30 ppm; Pore Size 150 Å; Specific Surface Area 240 m2/g; Carbon Loading 15%; Double End-cap; Available
Particle Size: 5µm, 10 µm
Main Features:
●Wide pH range (1.5-12)
●Minimal silanol activity
●Better efficiency than many other high pH compatible columns
●High loading capacity for basic compounds for preparative applications
High pH Stability
Column: Durashell RP; 4.6 x 150 mm
Mobile Phase: 50% ACN/50% 0.05M Ammonia (pH=9.0)
Flow rate: 1.5 mL/min
Sample: doxepin, nortriptyline, amitriptyline,
trimipramine ,
Temperature: 35oC
Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556
NEW in HPLC !
Initial
After 100 h
0.70
0.60
0.50
0.40
0.30
0.20
0.10
0.00
-0.10
2.00 4.00 6.00 8.00 10.00 12.00 14.00 18.00
HP
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AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
Ordering Information
Type Particle size (µm) Dimension (mm) Durashell RP
Surface Area: 240m2/g, Pore Size: 150Å
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Guard cartridge
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Guard cartridge
Semi-preparative
Semi-preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Guard cartridge
Semi-preparative
Semi-preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Guard cartridge
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
3
5
5
5
5
5
5
5
5
5
10
10
10
10
10
10
10
10
10
10
10
DS950302-0
DS950502-0
DS951002-0
DS951502-0
DS950505-0
DS951005-0
DS951505-0
DS952005-0
DS952505-0
DS950105-0
DS930302-0
DS930502-0
DS931002-0
DS931502-0
DS930505-0
DS931005-0
DS931505-0
DS930105-0
DS951510-0
DS952510-0
DS950520-0
DS951520-0
DS952520-0
DS951030-0
DS951530-0
DS952530-0
DS950220-0
DS901510-0
DS902510-0
DS900520-0
DS901520-0
DS902520-0
DS901030-0
DS901530-0
DS902530-0
DS901550-0
DS902550-0
DS900220-0
2.1×30
2.1×50
2.1×100
2.1×150
4.6×50
4.6×100
4.6×150
4.6×200
4.6×250
4.6×10
2.1×30
2.1×50
2.1×100
2.1×150
4.6×50
4.6×100
4.6×150
4.6×10
10×150
10×250
20×50
20×150
20×250
30×100
30×150
30×250
20×20
10×150
10×250
20×50
20×150
20×250
30×100
30×150
30×250
50×150
50×250
20×20
HP
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Best ValueGuaranteed Product QualityInnovation to Benefit Customers
Venusil Family of HPLC Columns Full Line of High Quality Products
Introduction of Venusil HPLC Columns
Venusil HPLC columns are manufactured from the highest purity spherical silica particles on the market. The outstanding properties of the
HPLC phases are results of subjecting high quality silica to Agela’ s innovative surface modification and unique bonding processes. Our
proprietary nanosurface modification generates a very smooth and even surface. This reduces the interaction between the silica surface and
polar compounds, resulting in symmetric peak shape even for very basic compounds. Based on this technology, a series of unique columns
were developed to meet the tough requirements for the analysis of highly polar compounds. Lot-to-lot reproducibility is ensured by a stable
bonding/end-capping process. The uniform, spherical particles have a nominal surface area of 380m2/g, 200 m2/g or 45m2/g, with a controlled
pore size of 100Å, 150Å or 300Å, respectively. All columns are packed using a consistent slurry packing process to achieve uniform and
stable beds for maximum column efficiency, lifetime and column-to-column reproducibility.
Unprecedented Experience in Chromatography
You will experience unprecedented performance using Venusil columns for your HPLC separations
Best Peak Symmetry and Efficiency
The Venusil columns generate symmetric peaks with
high efficiency over their entire applicable pH range,
outperforming competitors columns.
The pH-independent high performance feature of
Venusil columns allows scientists to establish rugged
methods with flexible choice of pH.
Tightest Specification
All our columns have tighter specification (see the table) than other
brand-name columns. This ensures that all columns have great
performance and identical selectivity
Comparison of Specifications
Waters Symmetry-C18
4.6×150mm, 5µm
Zorbax XDB-C18
4.6×150mm ,5µm
Venusil XBP C18 (2)
4.6×150mm ,5µm
N/A
N/A
1.8
+/ 10%
2 8
300
16%
Spec. on efficiency
Spec. on tailing factor(toluene)
Spec. on tailing factor amitriptyline
Spec. on selectivity(amitriptyline/acenaphthene)
pH range
Specific Surface
Carbon%
8000
0.98 1.25
N/A(typically 1.5 2.0)
N/A
2 9
180
12%
12000
0.98 1.12
1.4
+/ 7%
1.5 9
380
19%
Characteristics of Venusil Columns Benefit to the Columns’ users
Perfect peak symmetry, independent of pH
and mobile phase
Broad pH range (1 10.5)
Compatible with 100% aqueous mobile phase
Highest surface coverage
A wide variety of chemistries
Minimum buffer concentration needed
Tightest specification
Excellent column efficiency
Great column lifetime
Highest flexibility for any application,better sensitivity,
greater confidence on HPLC results
Flexibility for method development
Maximum versatility
Tolerance of large volume and mass injection
Broad selection of polarity and maximum versatility
Better sensitivity for LC/MS, extended column lifetime
Better reproducibility, greater confidence on the HPLC results
Better and faster separation
Lower cost; greater convenience
Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556
-
HP
LC
Colu
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AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
Expanding the Capabilities with the Venusil Family of C18 Columns
Many of our columns have very unique properties to address some special needs. Here are some examples:
Venusil XBP-C18 (2): a phase designed for balanced adsorption to avoid excessive retention of hydrophobic compounds,
• Great peak symmetry for all types of compounds,
• Improved separation of stereo isomers
• Extremely narrow specification during manufacture to offer high column-to-column reproducibility
• Non-excessive retention for hydrophobic peaks (less peak broadening of later eluting compounds compared to
other columns)
Venusil ASB C18: a phase designed for low pH, low bleed (high sensitivity for LC-MS) and strong separation power for polar
compounds:
• Extremely low pH stability: pH limit =0.8 at 70
• Extremely low bleed offering high sensitivity for LC-MS under acidic conditions
• Compatible with 100% aqueous to 100% organic mobile phases
• Non-endcapped but with low surface acidity/activity compared to other non-endcapped stationary phases
Venusil AQ C18: a phase designed for polar and semi-polar compounds, to be compatible with 100% water :
• Compatible with 100% aqueous to 100% organic mobile phases
• Applicable to a variety of analytes: from very polar to non-polar
• Operates over a wide pH range: 1.0-9.0
• Applicable to a wide range of sample types: plasma, urine, drug formulation, food extraction
• Available in a range of column diameters suitable for LC-MS, conventional analytical, and preparative scale
Venusil XBP-C18: a phase designed for maximum hydrophobicity and high pH tolerance:
• The highest carbon loading and the most hydrophobic column on the market.
• High pH tolerance
• Not suggested for samples containing highly hydrophobic compounds
Venusil XBP-C18 (L): a phase designed for larger molecules and highly hydrophobic compounds:
• Larger pore size and lower surface area
• Accelerated elution for highly hydrophobic compounds
• Easier column clean-up for samples containing hydrophobic impurities or samples extracted by non-polar solvents
• Better choice for molecules > 500 Dalton
Agela Technologies has developed a series of high quality C18 stationary phases to meet a wide range of application needs.These columns
contain an ultra pure silica subjected to our patented surface deactivation process. By altering the column chemistry, we are able to tune the
surface properties of the silica particles and alter selectivity to meet a variety of application needs.
HP
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Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556
Best ValueGuaranteed Product QualityInnovation to Benefit Customers
Hydrophobicity Test
Inertness Test
Venusil XBP C18 (2) Series Columns
Universal, Highly Inert C18 Columns
• Balanced hydrophobic adsorption which results in a broader
suitability
• Stronger separation power for isomers
• Great column-to-column and batch-to-batch reproducibility
• Perfect symmetry for basic, acidic and neutral compounds
A good replacement of Luna C18 (2) columns!
Technical Parameters
• Metal Impuriy < 10ppm
• Particle Size: 5μm, 3μm, 10μm
• Specific Surface: 380m2/g
• Pore Size: 100Å
• Carbon Loading: 19%
• Double End-cap
• pH Range: 1.5-9.0
• Performance: Efficiency 80,000/m (5μm)
• TF: 0.98-1.12
Column: Venusil XBP C18 (2), 4.6 × 150mm, 5 μm
Sample: Uracil, Toluene
Mobile phase: MeOH: 25 mM KH2PO
4/K
2HPO
4 (pH 6.0) = 80 : 20
Detection: UV 254 nm
Flow rate: 1mL/min
Venusil XBP C18 (2) column packing material is made with ultra pure silica. The silica surface is processed with Agela’ s patented surface
deactivation technology, followed by a unique bonding process that can reduce the carbon content while maintaining a high bonding cover-
age. The Venusil XBP C18 column does not have excessive retention for highly hydrophobic compounds, and it is great for the separation
of acidic, basic, and neutral compounds. Moreover, this column also has superb resolution power for isomers. The perfect peak symmetry
offered by this RP column makes it a great first-choice for HPLC method development.
Column: Venusil XBP C18 (2), 4.6 × 150mm, 5 μm
Sample: Amitriptyline
Mobile phase: NaH2PO
4/Na
2HPO
4 20mM, pH 6.9 :
MeOH = 29 : 71
Detection: UV 254 nm
Flow rate: 1mL/min
Amitriptyline is a strong basic antidepressant. It is recognized as
a compound difficult to obtain good peak shape;
But the XBP C18 2 column performed outstandingly and gave
a great peak symmetry TF = 1.12 .
min0 1 2 3 4 5
mAU
0
20
40
60
80
100
120
1.6
81
4.7
11
HP
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AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
Selectivity of Basic Compounds
Venusil XBP C18(2),4.6×150mm,5 μm
Part No.: VX951505-2
Brand Z C18 column 4.6×150mm,5 μm
Brand H C18 column 4.6×150mm,5 μm
Mobile Phase: MeOH Acetic Acid/ sodium acetate aq.
(pH 5.0 =60 40
Flow Rate 1 mL/min
Temperature 30
Detection UV 254 nm
Sample p-Methyl benzoic acid, Pyridine,Phenol,
Acetophenone, Amino-dimethyl
benzene, Toluene
Extremely Low Metal Effects
Mobile Phase: Water :Methanol=65:35
Flow Rate: 1 mL/min
Temperature: 35
Detection: UV 230 nm
Sample: 2,3-Dihydroxynaphthalene 2,7
-Dihydroxynaphthalene
Venusil XBP C18(2) 4.6×150mm
Part.No.:VX951505-2
Brand Y C18 4.6×150mm
Brand H 4.6×150mm
Ordering Information
Surface Area: 380m2/g,Pore Size: 100Å
Type Dimension (mm) Particle size(µm) Venusil XBP-C18 (2)
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Guard cartridge
2.1×30
2.1×50
2.1×100
2.1×150
4.6×50
4.6×100
4.6×150
4.6×250
4.6×10 4/pk
5
5
5
5
5
5
5
5
5
VX950302-2
VX950502-2
VX951002-2
VX951502-2
VX950505-2
VX951005-2
VX951505-2
VX952505-2
VX950105-2
HP
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Best ValueGuaranteed Product QualityInnovation to Benefit Customers
Comparison of Venusil-C8 Phases
Plates (Toluene) Tailing Factor
3805
2546
4012
Brand Z-C8
Luna C8
Venusil XBP-C8
1.12
1.15
1.03
Sample: uracil, phenol,chloronitrobenzene,
toluene(in methanol/water=50/50)
Column: 2.1 x 50 mm, 5 μm
Mobile phase: methanol: water = 65: 35
Flow rate: 0.20 mL/min
Temperature: 30 oC
Sample: Uracil, Doxepin, Nortriptyline,Amitriptyline,
Trimipramine
Column: Venusil XBP-C18, 4.6 x 150 mm, 5 μm
Mobile phase: 0.01 M sodium phosphate: ACN= 50 : 50, pH=7.6
Flow rate: 1.5 mL/min
Temperature: 45 oC
Venusil XBP Series Columns
Venusil XBP C18
Venusil XBP C18 columns have the maximum bonding
density therefore the highest hydrophobicity
(the lowest polarity). This allows for the least interaction
between the analytes and the silanol groups. Venusil XBP
columns have extraordinary column stability at high pHs.
Pore size: 100Å, 300 Å; Specific surface: 380 m2/g,
45m2/g; Purity > 99.999%;
1) Inertness: one of most base-friendly columns.
2) Efficiency: excellent at any applicable pH.
3) Large volume injection: maintain very high efficiency with
very large injection volumes.
4) Broad pH range (1.5 – 10.0 ): low pH, 0.2 % TFA; high pH,
0.010 M triethylamine.
Other Venusil XBP Columns: A complete line of stationary
phases include C8, C4, C1, NH2, Phenyl, CN, Silica, SCX,
SAX;
Quality of Column Packing Perfect Peak Symmetry and Great Lifetime of
the Column for Basic Compounds at Mid-pH
Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556
0.6
73
1.0
31
2.0
16
2.9
04
Brand Z C8
0.7
41
1.1
87
2.7
37
3.8
32
Venusil XBP C8
0.7
38
1.1
90
2.4
07
3.4
59
Luna C8
Initial
After 10 days
0.6
07
0.7
01
0.6
18 0.8
58
3.8
22
6.0
47
10.2
57
17.0
21
0.7
27
0.8
55
3.3
84
5.8
10
9.7
76
16.7
55
HP
LC C
olum
ns
022
AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
Ordering Information
Surface Area: 380m2/g,Pore Size: 100Å
Type Dimension(mm) Particle(μm) Venusil XBP-C18 Venusil XBP-C8 Venusil XBP-C4 Venusil XBP-C1
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Guard cartridge
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Guard cartridge
Semi-preparative
Semi-preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Guard cartridge
Semi-preparative
Semi-preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Guard cartridge
2.1×30
2.1×50
2.1×100
2.1×150
4.6×50
4.6×100
4.6×150
4.6×200
4.6×250
4.6×10 4/pk
2.1×30
2.1×50
2.1×100
2.1×150
4.6×50
4.6×100
4.6×150
4.6×10 4/pk
10×150
10×250
20×50
20×150
20×250
30×100
30×150
30×250
20×10
10×150
10×250
20×50
20×150
20×250
30×100
30×150
30×250
50×150
50×250
20×10
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
3
5
5
5
5
5
5
5
5
5
10
10
10
10
10
10
10
10
10
10
10
VX950302-0
VX950502-0
VX951002-0
VX951502-0
VX950505-0
VX951005-0
VX951505-0
VX952005-0
VX952505-0
VX950105-0
VX930302-0
VX930502-0
VX931002-0
VX931502-0
VX930505-0
VX931005-0
VX931505-0
VX930105-0
VX951510-0
VX952510-0
VX950520-0
VX951520-0
VX952520-0
VX951030-0
VX951530-0
VX952530-0
VX950120-0
VX901510-0
VX902510-0
VX900520-0
VX901520-0
VX902520-0
VX901030-0
VX901530-0
VX902530-0
VX901550-0
VX902550-0
VX900120-0
VX450302-0
VX450502-0
VX451002-0
VX451502-0
VX450505-0
VX451005-0
VX451505-0
VX452005-0
VX452505-0
VX450105-0
VX150302-0
VX150502-0
VX151002-0
VX151502-0
VX150505-0
VX151005-0
VX151505-0
VX152005-0
VX152505-0
VX150105-0
VX850302-0
VX850502-0
VX851002-0
VX851502-0
VX850505-0
VX851005-0
VX851505-0
VX852005-0
VX852505-0
VX850105-0
VX830302-0
VX830502-0
VX831002-0
VX831502-0
VX830505-0
VX831005-0
VX831505-0
VX830105-0
VX851510-0
VX852510-0
VX850520-0
VX851520-0
VX852520-0
VX851030-0
VX851530-0
VX852530-0
VX850120-0
VX801510-0
VX802510-0
VX800520-0
VX801520-0
VX802520-0
VX801030-0
VX801530-0
VX802530-0
VX801550-0
VX802550-0
VX800120-0
Surface Area: 380m2/g,Pore Size: 100Å
Dimension(mm)
Particle(μm)
VenusilXBP-NH
2
VenusilXBP-Phenyl
VenusilXBP-CN
VenusilSilica
Type
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Guard cartridge
2.1×30
2.1×50
2.1×100
2.1×150
4.6×50
4.6×100
4.6×150
4.6×200
4.6×250
4.6×10 4/pk
5
5
5
5
5
5
5
5
5
5
VN850302-0
VN850502-0
VN851002-0
VN851502-0
VN850505-0
VN851005-0
VN851505-0
VN852005-0
VN852505-0
VN850105-0
VC950302-0
VC950502-0
VC951002-0
VC951502-0
VC950505-0
VC951005-0
VC951505-0
VC952005-0
VC952505-0
VC950105-0
VSi950302-0
VSi950502-0
VSi951002-0
VSi951502-0
VSi950505-0
VSi951005-0
VSi951505-0
VSi952005-0
VSi952505-0
VSi950105-0
VX650302-0
VX650502-0
VX651002-0
VX651502-0
VX650505-0
VX651005-0
VX651505-0
VX652005-0
VX652505-0
VX650105-0
HP
LC C
olum
ns
023
Best ValueGuaranteed Product QualityInnovation to Benefit Customers
Surface Area: 45m2/g,Pore Size: 300Å
Dimension(mm)
Particle(μm)
VenusilXBP-C18
VenusilXBP-C8
VenusilXBP-C4
VenusilXBP-C1
Type
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Guard cartridge
Semi-preparative
Semi-preparative
Preparative
Preparative
Preparative
Guard cartridge
2.1×30
2.1×50
2.1×100
2.1×150
4.6×50
4.6×100
4.6×150
4.6×200
4.6×250
4.6×10 4/pk
10×150
10×250
20×50
20×150
20×250
20×10
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
VX950302-T
VX950502-T
VX951002-T
VX951502-T
VX950505-T
VX951005-T
VX951505-T
VX952005-T
VX952505-T
VX950105-T
VX951510-T
VX952510-T
VX950520-T
VX951520-T
VX952520-T
VX950120-T
VX450302-T
VX450502-T
VX451002-T
VX451502-T
VX450505-T
VX451005-T
VX451505-T
VX452005-T
VX452505-T
VX450105-T
VX150302-T
VX150502-T
VX151002-T
VX151502-T
VX150505-T
VX151005-T
VX151505-T
VX152005-T
VX152505-T
VX150105-T
VX850302-T
VX850502-T
VX851002-T
VX851502-T
VX850505-T
VX851005-T
VX851505-T
VX852005-T
VX852505-T
VX850105-T
VX851510-T
VX852510-T
VX850520-T
VX851520-T
VX852520-T
VX850120-T
Surface Area: 45m2/g,Pore Size: 300Å
Dimension(mm)
Particle(μm)
VenusilXBP-NH
2
VenusilXBP-Phenyl
VenusilXBP-CN
VenusilSilica
Type
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Guard cartridge
2.1×30
2.1×50
2.1×100
2.1×150
4.6×50
4.6×100
4.6×150
4.6×200
4.6×250
4.6×10 4/pk
5
5
5
5
5
5
5
5
5
5
VN850302-T
VN850502-T
VN851002-T
VN851502-T
VN850505-T
VN851005-T
VN851505-T
VN852005-T
VN852505-T
VN850105-T
VC950302-T
VC950502-T
VC951002-T
VC951502-T
VC950505-T
VC951005-T
VC951505-T
VC952005-T
VC952505-T
VC950105-T
VSi950302-T
VSi950502-T
VSi951002-T
VSi951502-T
VSi950505-T
VSi951005-T
VSi951505-T
VSi952005-T
VSi952505-T
VSi950105-T
VX650302-T
VX650502-T
VX651002-T
VX651502-T
VX650505-T
VX651005-T
VX651505-T
VX652005-T
VX652505-T
VX650105-T
Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556
HP
LC C
olum
ns
024
AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
Venusil XBP (L) Series Columns
Surface Area: 200m2/g,Pore Size: 150Å
Dimension(mm)
Particle(μm)
Type
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
2.1×30
2.1×50
2.1×100
2.1×150
4.6×50
4.6×100
4.6×150
4.6×250
5
5
5
5
5
5
5
5
VenusilXBP(L) C18
VX950302-L
VX950502-L
VX951002-L
VX951502-L
VX950505-L
VX951005-L
VX951505-L
VX952505-L
VenusilXBP(L) Silica
VSi950302-L
VSi950502-L
VSi951002-L
VSi951502-L
VSi950505-L
VSi951005-L
VSi951505-L
VSi952505-L
VenusilXBP(L) C8
VX850302-L
VX850502-L
VX851002-L
VX851502-L
VX850505-L
VX851005-L
VX851505-L
VX852505-L
XBP (L) C18 has an identical bonded phase as XBP C18. However, XBP (L) C18 has relatively low surface area which allows
compounds to have shorter retention times. These columns are an excellent alternative to Hypersil BDS-C18, Hypersil BDS-C8;
Zorbax XDB-C18, Zorbax XDB-C8 which comparitively could have too much retention.
XBP (L) C18 have relatively larger pore size (150Å) and are suitable for the separation of large molecules.
StationaryPhase
Venusil
XBP-C18(2)
Venusil
XBP-C18
Venusil
XBP-C18(L)
Venusil
AQ C18
Venusil
ASB-C18
Specific SurfaceArea (m2/g)
Pore Size(Å)
CarbonLoading
Particle SizeAvailable (μm)
pHRange
Characteristics
380
380
200
380
200
100
100
150
100
150
19%
22%
15%
18%
12%
3, 5, 10
3, 5, 10
3, 5, 10
3, 5, 10
3, 5, 10
1.5
1.5
1.5
1.0
0.8
Balanced adsorption to avoid excessive reten-tion; a wider range of suitability; perfect peak symmetry for acidic, basic and neutral com-pounds; greater separation power for isomers.
The most hydrophobic adsorption; better high pH tolerance; not suggested for samples which contain highly hydrophobic compounds.
Larger pore size and lower surface area;a better choice for the analysis of large molecule and strong hydrophobic compounds.
Slightly polar C18 and enhanced retention for polar compounds; 100% water compatible; good peak shape for acidic, basic and neutral compounds.
Polar C18; extremely low bleed at low pH; great for LC-MS and peptide separation; stable at pH as low as 0.8 at 70 .
HP
LC C
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025
Best ValueGuaranteed Product QualityInnovation to Benefit Customers
Venusil AQ-C18 Columns
The Venusil AQ-C18 column is designed for the separation of polar, semi-polar and non-polar compounds at low to medium pH. This column
is more polar than XBP-C18, but less polar than ASB-C18. With a special surface treatment, Venusil AQ-C18 is made to be compatible with
100% aqueous mobile phases., and it can be used to replace Waters Symmetry, Inertsil C18, Zorbax SB, Atlantis dC18, and other AQ
columns. This column has a pH range of 1.5-9.0.
Pore size: 100 Å; Specific surface: 380 m2/g; Purity > 99.999%;
Compatibility with 100% Aqueous Mobile Phase
Sample: Ranitidine
Column: 4.6×150mm,5μm
Mobile phase: 100% Water
Flow rate: 1mL/min; the flow was stopped for 5 minutes
during the testing period for each column.
Temperature: 30
4) 100% water compatible: very unique for a universal C18
column; much better peak shape, retention, and efficiency
than other brand AQ columns.
5) Low pH stability (pH=1.5):better stability than most of the
popular brand columns (including other brand AQ columns)
on the market.
1) Inertness: one of most base-friendly columns, excellent
peak symmetry of basic compounds.
2) Efficiency: The best column for polar compounds at any pH.
3) Large volume injection: maintains very high efficiency even
if the injection volume is exceptionally large.
Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556
HP
LC
Colu
mns
026
AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
Balanced Retention for Hydrophilic and Hydrophobic Compounds
Mobile Phase: 35% v/v 20.0 mM KH2PO
4/K
2HPO
4
at pH7.00/65% Methanol at 23
Flow: 1mL/min
Detection: UV 254nm
Sample: Uracil Propranolol hydrochloride
Butyl ,Phthalate Dimethyl Phthalate
Naphthalene ,Acenaphthene Amitriptyline
Mobile Phase 1% Acetic Acid (pH=2.57) :MeOH =50:50
Flow 1mL/min
Temperature 30
Detection 275nm
Sample Uracil Paracetamol acetophenone and
2-Hydroxybenzoic acid
Great Peak Shape for All Type of Compounds
min0 2 4 6 8 10 12 14 16 18
mAU
0
100
200
300
400
VWD1 A, Wavelength=275 nm (HLX\08021218.D)
2.8
09
3.5
44
10
.88
4
11
.77
0
Venusil AQ Column,4.6×250mm
Part No.: VA952505-0
VWD1 A, Wavelength=275 nm (HLX\08021217.D)
2.9
44
3.5
21
9.4
85
11
.41
3
Brand Y AQ Column,4.6×250mm
mAU
0
100
200
300
400
min0 2 4 6 8 10 12 14 16 18
Venusil AQ ,4.6×100mm,
Part No.: VA951005-0
Brand Y AQ ,4.6×100mm
HP
LC C
olum
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027
Best ValueGuaranteed Product QualityInnovation to Benefit Customers
Ordering Information
Surface Area: 380m2/g,Pore Size: 100Å
Dimension(mm)
Particle(μm)
VenusilAQ-C18
Type
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Guard cartridge
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Guard cartridge
Semi-preparative
Semi-preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Guard cartridge
2.1 30
2.1 50
2.1 100
2.1 150
4.6 50
4.6 100
4.6 150
4.6 250
4.6 10 4/pk
2.1 30
2.1 50
2.1 100
2.1 150
4.6 50
4.6 100
4.6 150
4.6 10 4/pk
10 150
10 250
20 50
20 150
20 250
30 100
30 150
30 250
20 10
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
3
5
5
5
5
5
5
5
5
5
VA950302-0
VA950502-0
VA951002-0
VA951502-0
VA950505-0
VA951005-0
VA951505-0
VA952505-0
VA950105-0
VA930302-3
VA930502-0
VA931002-0
VA931502-0
VA930505-0
VA931005-0
VA931505-0
VA930105-0
VA951510-0
VA952510-0
VA950520-0
VA951520-0
VA952520-0
VA951030-0
VA951530-0
VA952530-0
VA950120-0
Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556
HP
LC C
olum
ns
028
AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
The Venusil ASB column is specially designed for the separation of polar compounds from low (extremely stable at pH=1) to medium pH. The
stationary phase is end-capped with polar groups and thus has the highest polarity among all Venusil family columns. This column can be
used to replace Water Atlantis, Zorbax SB, and other brand AQ columns.
Pore size: 150Å; Specific surface: 200m2/g; Purity 99.999%
min
Column: Venusil ASB C18, 5μm, 4.6×150mm
Sample: Naphthol
Aging: 40 , TFA in 80% methanol (pH=1.0), 400 hours
Mobile Phase:TFA in 80% methanol (pH=1.5)
Flow Rate:1 mL/min
Injection: 5 μL
Temperature:30
Venusil ASB Columns
1) Low pH stability: great stability even at pH=1
2) Peak shape and efficiency: Excellent peak symmetry
for basic compounds comparing to other brand polar
phase columns
3) Polar C18 phase: Very strong separation power for
polar compounds
4) 100% aqueous compatible: Much better peak shape,
retention, and efficiency comparing to other brand “AQ”
columns.
The Stability in the Low pH
The separation of Organic Acid
Column: Venusil ASB C18, 5μm, 4.6×150mm
Sample: Vc, malonic acid, lactic acid, citric acid
Mobile Phase:20 mM Phosphate buffer saline (PBS) , pH=2.0
Flow Rate:1 mL/min
Injection: 5 μL
Detection:UV 210 nm
0
0 1 2 3 4 5 6 7 8 9
10
20
30
40
50
mAU
0
20
40
60
80
100
140
120
mAU
min
0 1 2 3 4 5 6 7 8 9 min
0 1 2 3 4 5 6 7 8
0
5
10
15
20
25
30
40
35
mAU
VWD1A,Wavelength=210nm(ZZX\0503-046.D)
Area:21.2573
HP
LC C
olum
ns
029
Best ValueGuaranteed Product QualityInnovation to Benefit Customers
Ordering Information
Surface: 200m2/g,Pore size: 150Å
Dimension(mm)
Particle(μm)
VenusilASB-C18
Type
Type
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Guard cartridge
Semi-preparative
Semi-preparative
Preparative
Preparative
Preparative
Guard cartridge
2.1 30
2.1 50
2.1 100
2.1 150
4.6 50
4.6 100
4.6 150
4.6 250
4.6 10 4/pk
10 150
10 250
20 50
20 150
20 250
20 10
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
VS950302-0
VS950502-0
VS951002-0
VS951502-0
VS950505-0
VS951005-0
VS951505-0
VS952505-0
VS950105-0
VS951510-0
VS952510-0
VS950520-0
VS951520-0
VS952520-0
VS950120-0
VenusilASB-C8
VS850302-0
VS850502-0
VS851002-0
VS851502-0
VS950505-0
VS851005-0
VS851505-0
VS852505-0
VS850105-0
VS851510-0
VS852510-0
VS850520-0
VS851520-0
VS852520-0
VS850120-0
VenusilASB-Phenyl
VS650302-0
VS650502-0
VS651002-0
VS651502-0
VS650505-0
VS651005-0
VS651505-0
VS652505-0
VS650105-0
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytica
Guard cartridge
2.1 30
2.1 50
2.1 100
2.1 150
4.6 50
4.6 100
4.6 150
4.6 250
4.6 10 4/pk
5
5
5
5
5
5
5
5
5
VS950302-T
VS950502-T
VS951002-T
VS951502-T
VS950505-T
VS951005-T
VS951505-T
VS952505-T
VS950105-T
VS850302-T
VS850502-T
VS851002-T
VS851502-T
VS850505-T
VS851005-T
VS851505-T
VS852505-T
VS850105-T
Surface Aera: 45m2/g,Pore Size: 300Å
Dimension(mm)
Particle(μm)
VenusilASB-C18
VenusilASB-C8
Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556
HP
LC C
olum
ns
030
AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
Venusil SCX HPLC Columns
SCX HPLC Column: made of spherical silica particles of the highest purity (>99.999%), bonded with aromatic sulfonic acid
group.They can be used forthe separation of basic, water-soluble compounds and bio-molecules.
Surface Aera: 200m2/g,Pore Size: 150Å
Dimension(mm)
Particle(μm)
VenusilSCX
Type
Analytical
Analytical
Analytical
Guard cartridge
4.6×100
4.6×150
4.6×250
4.6×10 4/pk
5
5
5
5
VSc951005-0
VSc951505-0
VSc952505-0
VSc950105-0
Ordering Information
Surface Aera: 50m2/g,Pore Size: 300Å
Dimension
(mm)
Particle
(μm)
Venusil
SCX Type
Analytical
Analytical
Analytical
Guard cartridge
4.6×100
4.6×150
4.6×250
4.6×10 4/pk
5
5
5
5
VSc951005-T
VSc951505-T
VSc952505-T
VSc950105-T
Venusil SAX HPLC Columns
SAX HPLC Column: the stationary phase is made with high purity spherical silica particles and bonded with quaternary amine groups.
The column can be used for the separation of acidic, water-soluble compounds and bio-molecules
Surface Aera: 200m2/g,Pore Size: 150Å
Dimension(mm)
Particle(μm)
VenusilSAX
Type
Analytical
Analytical
Analytical
Guard cartridge
4.6×100
4.6×150
4.6×250
4.6×10 4/pk
5
5
5
5
VSa951005-0
VSa951505-0
VSa952505-0
VSa950105-0
Ordering Information
Surface Aera: 50m2/g,Pore Size: 300Å
Dimension
(mm)
Particle
(μm)
Venusil
SAX Type
Analytical
Analytical
Analytical
Guard cartridge
4.6×100
4.6×150
4.6×250
4.6×10 4/pk
5
5
5
5
VSa951005-T
VSa951505-T
VSa952505-T
VSa950105-T
HP
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Best ValueGuaranteed Product QualityInnovation to Benefit Customers
Promosil HPLC Columns
• Double End-cap, Good Inertness
• Good Stability and Reproducibility
• Excellent Performance-to-cost Value
Using the silica of high-purity and high mechanical strength, the Promosil C18s are made with high-purity monosilane through Agela
Technologies’ well controlled bonding process. They have high surface bonding coverage and completely capped. The carbon content
is as much as 18%. They are stable at pH range 1.5-9.0 showing good peak shape for acidic and basic compounds. They have excellent
tolerance of contamination and long life-time. They are the best choice of high performance-to-cost value.
Technical Parameters:
• High-purity silica, Metal Impurity<10ppm
• Average Particle Size: 5μm
• Specific Surface: 320m2/g
• Pore Size: 100Å
• Carbon Loading: 18%
• pH Range: 1.5-9.0
• Efficiency: >80, 000/m
• TF: 0.98-1.20
Hydrophobicity Test
Column: Promosil C18, 4.6 × 150mm, 5 μm
Sample: Uracil, Toluene
Mobile phase: MeOH: 25 mM KH2PO
4/K
2HPO
4 ( pH 6.0 ) = 80 : 20
Detection: UV 254 nm
Flow rate: 1mL/min min0 1 2 3 4 5
mAU
0
20
40
60
80
100
120
140
1.4
96
4.3
48
Inertness Test
Column: Promosil C18, 4.6 × 150mm, 5 μm
Sample: Amitriptyline
Mobile phase: NaH2PO
4/Na
2HPO
4 (20mM, pH 6.9): MeOH = 32 : 68
Detection: UV 254 nm
Flow rate: 1mL/min
Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556
mAU
-100
-50
0
50
100
150
200
1.6
67 5
.44
0
2.9
23
1.8
70
2.4
28
7.3
35
7.8
09
9.8
78
19
.97
4
34
.15
5
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AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
Other Manufacturers’ Brands
Agela Technologies may also supply a number of major brand name HPLC columns made by other manufacturers, as listed below. Please
contact us for availability and price information..
These brands are: Zobax, Kromasil, Hypersil, ES-Industry, Shodex, Jordi
Ordering Information
Dimension(mm)
2.1 30
2.1 50
2.1 100
2.1 150
4.6 50
4.6 100
4.6 150
4.6 250
4.6 10, 4/pk
Particle(μm)
5
5
5
5
5
5
5
5
5
PromosilC18
PM950302-0
PM950502-0
PM951002-0
PM951502-0
PM950505-0
PM951005-0
PM951505-0
PM952505-0
PM950105-0
PromosilC8
PM850302-0
PM850502-0
PM851002-0
PM851502-0
PM850505-0
PM851005-0
PM851505-0
PM852505-0
PM850105-0
PromosilNH
2
PM750302-0
PM750502-0
PM751002-0
PM751502-0
PM750505-0
PM751005-0
PM751505-0
PM752505-0
PM750105-0
PromosilCN
PM550302-0
PM550502-0
PM551002-0
PM551502-0
PM550505-0
PM551005-0
PM551505-0
PM552505-0
PM550105-0
PromosilSilica
PM050302-0
PM050502-0
PM051002-0
PM051502-0
PM050505-0
PM051005-0
PM051505-0
PM052505-0
PM050105-0
Surface area: 320m2/g Pore size: 100Å
HP
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Selected Solutions for Popular Applications
Solutions for Highly Water Soluble Compounds:
Venusil AQ C18
Venusil ASB C18
Unisol Amide
It is known that highly water-soluble compounds often have difficulties in HPLC analysis, such as lack of retention, bad peak shape and inconsistent
results. A large scientific effort has been made in developing HPLC stationary phases that can provide solutions to these problems. However, no
single stationary phase may resolve all these difficulties. Agela Technologies has developed a series of stationary phases along with development
schemes to help chemists systematically develop methods for the analysis of highly water-soluble compounds. The series includes two reverse
stationary phases which are compatible with 100% water and one strong hydrophilic interaction stationary phase which has a neutral functionality on
its surface. Some application examples are included as a guide to your column selection.
Venusil AQ-C18 is a slightly-polar C18 column, and it is our most versatile high aqueous reverse phase column. Venusil AQ-C18 has a broad pH
suitability (1.5-9.0) and thus can be your first option for most of your HPLC applications. Venusil ASB-C18 has pH range of 0.8-7.0. The column
stationary phase is non-end capped polar C18 and good for low pH conditions. Unisol Amide (HILIC) has a pH range of 2-8 . Unisol Amide column
stationary phase is modified with strong hydrophilic and neutral functionalities, it has hydrophilic interaction with the compounds. Unisol Amide
provides the strongest retention to hydrophilic compounds among these three types of columns.
In general, your method development may start with a Venusil AQ-C18 column and a mobile phase containing a mixture of methanol or acetonitrile
with an aqueous acidic buffer solution (pH=2-5). This approach may be applicable to the HPLC analysis of more than 50% of small molecules
(<2000 daltons). If required, you can minimize the percentage of the stronger organic mobile phase to increase retention by using up to 100% aque-
ous acidic buffer mobile phase. In the cases that you achieve adequate retention, but not enough resolution for the compounds of your interest, you
may chose to adjust mobile phase ratio, mobile phase pH (very effective for ionizable compounds), or chose to replace the column with a Venusil
ASB-C18 or Unisol Amide for an alternative selectivity. Figure 1 shows an HPLC separation of four organic acids using Unisol C18 column in 100%
aqueous mobile phase at pH=2.
In the cases that you cannot achieve adequate retention after analysis in 100% aqueous mobile phase using the Venusil AQ-C18, you have the
following options depending on the type of compounds. For acidic compounds, you may try ASB-C18 columns and lower the pH to further reduce
the solubility of the compounds in water. For such low pH applications, TFA is the preferred acidic modifier. TFA may give you the highest retention
of both acidic and basic compounds than other organic or inorganic acidic modifiers. If necessary, you may also try a Unisol Amide column in HILIC
mode for even higher retention and/or different selectivity. When using a Unisol Amide column, it will behave like a NP column. More polar com-
pounds will elute later than less polar compounds. Figure 2 shows a good separation of highly water-soluble shikimic acid and related substances
using a Unisol Amide column. The retention of these compounds was not adequate for analysis on either an AQ-C18 or an ASB-C18 column.
For basic compounds, you may start with a AQ- C18 column. For compounds with pKa > 9, another alternative is to use a Unisol Amide column in
place of the AQ- C18 column and operate at a low to medium pH (2-7). Figure 3 shows that even with a 100% aqueous mobile phase, validamycin
(basic and highly water soluable) still has poor retention on a AQ-C18 column. However, a Unisol Amide column operating at pH 2 in the hydrophilic
interaction mode resulted in a suitable validamycin separation.
For neutral compounds, changing pH will have a limited effect on retention. If a suitable method cannot be developed using an AQ-C18 column, an
ASB-C18 or Unisol Amide column may be required.
Usually during method development, the desired retention is achieved first, then the mobile phase pH and ratio is further adjusted to get the desired
resolution. When the desired retention or resolution cannot be obtained for highly water soluble compounds using a AQ-C18 or ASB-C18 columns, a
Unisol Amide column will be a good alternative. Unisol Amide columns offer completely different selectivity from reverse phase columns and offer
much higher retentivity .
The multiple selection of columns and this comprehensive method development scheme for the separation of highly water-soluble compounds will
allow you to develop HPLC methods at ease.
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
HP
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AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
Sample: Vitamin C, malonic acid, lactic acid, citric acid.
Column: AQ-C18, 4.6 x 150mm, 5 μm
Mobile phase 20mmol phosphate buffer pH=2.0
Detection UV 210nm
Flow rate: 1.0 mL/min
Temperature: 30oC
Column:Unisol Amide (HILIC) 4.6 x 250 mm, 5 μm
Mobile phase ACN/1% formic acid 90-60% in 20 min
Detection UV 210nm
Flow rate: 1.0 mL/min
Temperature: 30oC
Column:Unisol Amide (HILIC) 4.6 x 250 mm, 5 μm
Mobile phase ACN/0.1%TFA 85-40% in 30 min
Detection UV 210nm
Flow rate: 1.0 mL/min
Temperature: Ambient
Column:Waters Atlantis C18, 4.6 x 250 mm, 5 μm
Mobile phase TFA 1%
Flow rate: 1.0 mL/min
Figure 1. HPLC Separation of Organic Acids Figure 2. HPLC Analysis of Shikimic Acid
(3,4,5-Trihydroxy-1-cyclohexene-1-carboxylic Acid)
Figure 3. HPLC Analysis of Validamycin Raw Products
0 1 2 3 4 5 6 7 8 9
40
35
30
25
20
15
10
5
0
Area:
21.2
573
mA
U
min
2700
2200
1700
1200
700
200
2700
2200
1700
1200
700
2004
13
.17
0
12
.01
0
11
.39
7
10
.03
7
9.2
23
7.2
23
6.0
37
5.5
03
2.2
23
2.0
50
1.6
77
1.1
43
4.7
17
2
3.7
03
0 2 4 6 8 10 12 14m
AU
min
AU
2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00 18.00
0.12
0.10
0.08
0.06
0.04
0.02
0.00
0.02
min
0.50 1.00 1.50 2.00 2.50 3.00 3.50 4.00 4.50 5.00 5.50
2.00
1.80
1.60
1.40
1.20
1.00
0.80
0.60
0.40
0.20
0.00
-0.20
AU
min
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Other Stationary Phases from Agela Technologies for Hydrophilic Compounds Separation:
Unisol C18
Venusil XBP-Silica (HILIC II)
Venusil XBP-NH2 (HILIC III)
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
Acidic,basic or neutral
compounds
Good Retention, But Not Enough Resolution
Not E nough R etention
- VenusilAQ-C18 Columns
- MeOH/water or ACN/water
- pH=2-6 (TFA, formic acid or other buffer)
- adjust mobile phase ratio or pH
- use Venusil ASB-C18 or Venusil HILIC
Acidic
Compounds
Basic
Compounds
Neutral
Compounds
adjust pH to 9-10; or use Venusil HILIC
use Venusil ASB-C18 and adjust pH to1-2 ; or use Venusil HILIC
use Venusil HILIC and adjust pH to 3-7
HP
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AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
Venusil ASB C18
min0 1 2 3 4 5 6 7
mAU
0
50
100
150
200
250
DAD1 A, Sig=240,4 Ref=360,100 (AGELA\07052506.D)
3.8
39
4.5
74
3.6
13
Column: Venusil ASB C18 4.6×250 mm 5 μm (VS952505-0)
Mobile Phase 100mM NH4AC:ACN=95:5
Detection 240 nm
Flow rate 1mL/min
Temperature 40
Sample: Melamine in Feed
Sample preparated by SPE
(Cleanert PCX 60mg/3mL; Part No.: CX0603)
Unisol C18
Column: Unisol C18; 4.6 × 150 mm
Mobile Phase: A: NH4AC Buffer (Adjust pH 6.0)
B: ACN
Gradient: 10% B to 60% B in 20 min.
Flow rate: 1.0 mL/min
Sample: Teicoplanin+Impurity
Temperature: 35
Teicoplanin
Nicousamide
Column: Unisol C18 4.6×150mm ( UO951505-0)
Mobile Phase: Water:ACN:THF (50:50:1,0.1%H3PO
4)
Flow Rate: 1mL/min
Sample: Nicousamide
Detection: UV 254nm
OH
OH
OH
OH
OH OH
HOOC
HOHO
HOHOHO
HO
HN
NH
NH
NH
NH
NH2
HN
HN
HO HO
H3C
CI
NHR
CI
H
H H
H
OO
OO
O
O
O
O
O
O
O
O
O
O
R
O
A2-1
A2-2
A2-3
A2-4
A2-5
(Z)-4-decenoic acid8-methylnonanoic aicd n-decanoic aicd8-methyldecanioc aicd 9-methyldecanioc aicd
0.0
5.00 10.00 15.00 20.00 25.00 30.00
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
Time
NO2
CH O
CH3
O
O
NH
COOH
OH
11.49
16332865
Teicoplanin
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HILIC Column Family From Agela Technologies
Unisol Amide ( Agela HILIC )
Venusil XBP-Silica ( Agela HILIC II)
Venusil XBP-NH2 (Agela HILIC III)
Comparison:
●Selectivity
XBP-silica is slightly acidic (pH=5.6); while Unisol Amide is slightly basic (pH=7-9), and XBP-NH2 is more basic (pH=9.2).
XBP-silica has stronger retention of basic compounds, while Unisol Amide and XBP-NH2 have better retention of acidic compounds.
Unisol Amide has a balanced retention power for neutral, acidic and basic compounds, and thus the highest versatility.
●Reproducibility and Lifetime
Unisol Amide has the best reproducibility and lifetime because of a bonded and close-to-neutral protection layer.
Water-soluble Vitamins
●Selectivity comparison on 3 Columns
●Stability comparison of Unisol Amide and silica
Unisol Amide Column,
5μm, 4.6mm×150mm
Part No.: UA951505-0
XBP -NH2 Column,
5μm, 4.6×150mm
XBP-Silica Column,
5μm, 4.6×150mm
Mobile Phase: A: 0.1% TFA in Water
B: 0.1% TFA in Acetonitrile ; A:B =90:10
Flow Rate: 1 mL/min
Temperature: 30
Detection: UV 280 nm
Sample: VB1+VB6+VC+VB2
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
VB1 VB6
VB1
VC VB2
VB6
VC
VB2
VB1VB6
VCVB2
0 1 2 3 4 5 6
0 1 2 3 4 5 6
0 1 2 3 4 5 6
HP
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AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
Validamycin
OO
NH
CH2OH
HOH2C
OH
OH
CH2OH
OHHO
OH
OH
HO OH
Validamycin
Column: Unisol Amide,5μm, 4.6mm×250mm
Part No.: UA952505-0
Column:Brand W , AQ Column,5μm, 4.6mm×250mm
Mobile Phase: A: 0.1% TFA in Water
B: Acetonitrile
Gradient: 40% A to 85% A in 30 min
Flow Rate: 1 mL/min
Temperature: 25
Detection: UV 210 nm
Sample: Validamycin
Stability Comparison of Unisol Amide and Silica
Samples:VB1, VB6, VC, VB2
Aging Conditions:
MeOH:20 mM NaH2PO
4 (pH=7.00) = 40 : 60; 1.0mL/min Temp 40
HPLC Conditions:
Mobile Phase: 0.1%TFA : ACN=90:10
Detection: 280nm; Flow: 1.0 mL/min; Temp: 30?;Inj: 2 μL
Venusil HILIC, 4.6 × 150,5μm Brand W Silica, 4.6 × 150,5μm
Initial Initial
After agingAfter aging
VB1 VB2
VB6
VC
VB1 VB2
VB6
VC
VB1
VB2
VB6
VC
VB1
VB2
VB6
VC
HP
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Low pH Stability of ASB-C18
Column: Venusil ASB-C18, 4.6 ×150 mm, 5 μm
Sample: Naphthol
Aging: 40 , TFA in 80% methanol (pH=1.0), 400 hours
Mobile phase:TFA in 80% methanol (pH=1.5)
Flow rate: 1.0 mL/min
Injection: 5 μL
Temperature: 30
High pH Stability of Durashell RP
Initial
After 100 h
Column: Durashell RP ; 4.6 × 150 mm
Flow rate: 1.5 mL /min, 50% ACN/50% 0.05M
Ammonia (pH=9.0)
Sample: Doxepin, nortriptyline, amitriptyline,
trimipramine ,
Temperature: 35
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
Solutions for Low pH and High pH Applications
Venusil ASB-C18 (pH: 0.8-7.5);
Venusil ASB-C8 (pH: 1.0-7.5);
Durashell RP (pH: 1.5-12.0)
Agela Technologies offers a family of stationary phases for applications to be run at extremely low or extremely high pH. These columns
extend the capability of regular reverse phase HPLC columns with a typical pH range of 2-8, and provide more options for your applications
and method development needs.
1 2 3 4 5 6 7 8 90
1 2 3 4 5 6 7 8 90
0
10
20
30
40
50
0
20
40
60
80
100
120
140
-0.10
0.00
2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00
0.10
0.20
0.30
0.40
0.50
0.60
0.70
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AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
Solutions for LC-MSAgela Technologies provides columns that can meet the needs of LC-MS applications. The most popular LC-MS columns from Agela include:
●Unisol C18 (versatile)
●Venusil ASB-C18 and ASB-C8 (extremely low bleed and long lifetime at low pH)
●Unisol Amide (for extremely polar compounds)
Benefits:
●Low bleed and symmetric peak shape→ Sensitivity
●High retention for polar compounds → Sensitivity, versatility, and low ion suppression for bioanalytical analysis.
●Compatible with 100% water to 100% organic solvents → Simplified method development effort
●Stability Long column life means a reduced cost for customers
Pseudoephedrine in Plasma
Lignocaine
Pseudoephedrine
Pseudoephedrine
Mass system: API Qtrap 3200, Applied Biosystem
Mass condition: Positive Ion Mode, MRM
HPLC Column: Venusil ASB C18 Column 2.1mm×50mm
Part No.: VS950502-0
Sample: Sample prepared by SPE (Cleanert PCX
60mg/3mL; Part No.: CX0603)
Mobile Phase: A: 1% Formic Acid in Water B: Methanol
Gradient: 20%B to 95%B in 2 min, hold for 0.5 min, then
switch to A:B (20:80)
Flow Rate: 0.5 mL/min
Temperature: 25
CI
HC N N O
O
OH
2HCL
0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2Time.min
0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2Time.min
1.47
2.12
2.0e4
0.0
4.0e4
6.0e4
8.0e4
1.0e5
0.5e5
1.0e5
1.5e5
2.0e5
2.5e5
3.0e5
3.5e5
4.0e5
1.2e5
1.4e5
1.6e5
1.8e5
2.0e5
2.2e5
2.4e5
2.6e5
2.8e5
HP
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Analysis of 14 Kinds of Hormone
Mass system: Agilent 1100 Series LC/MSD Trap
HPLC Column: Venusil XBP C18 Column 2.1mm×150mm Part No.: VX951502-0
Sample: Estriol, Adosterone, Estrone+Estradial, Testosterone, Dehydro-iso-androsterone Methyl- Testosterone, Epitestosterone,
Medroxyprogestrone+Stanolone,Progesetrone, Androsterone, 5-pregen-3beta-20-one
Mobile Phase: A: Water. ;B: MeOH
Gradient: 40%B to 80%B in 24 min, then hold for 12 min.
Flow Rate: 0.8 mL /min
Temperature: 25
Oleic Acid in Plasma
Oleic acid
Mass system: API Qtrap 3200, Applied Biosystem
Mass condition: Negative Ion Mode, MRM
HPLC Column: Venusil ASB C18 Column 2.1mm×50mm Part No.: VS950502-0
Sample: Sample prepared by SPE (Cleanert PEP 60mg/3mL;Part No.: PE0603)
Mobile Phase: A: 13mmol/L ammonium acetate aq., B: Acetonitrile
Gradient: 5%B to 95%B in 2 min, hold for 2 min., switch to A:B (95:5) then hold for 2 min.
Flow Rate: 0.8 mL/min
Temperature: 25
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
HP
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AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
Solutions for Fast Analysis
Agela Technologies provides two approaches for fast HPLC analyses with shorter run times and higher column efficiencies.
●Small particle size or fused-core technology: Venusil AQ-C18 (2.5μm) , HALO (2.7μm)
●Unique retention mechanism : Optimix C18/Amide
HALO C18, 4.6×50mm, 2.7μm Mobile Phase: A: NH4AC Buffer (Adjust pH6.0)
B: ACN
Gradient: 5%B to 45%B in10 min.
Flow rate: 1.0 mL / min
Sample: Teicoplanin+Impurity
Temperature: 35
T=7min
Conventional C18, 4.6 × 150 mm
T=16min
Mobile Phase: A: NH4AC Buffer (Adjust pH 6.0)
B: ACN
Gradient: 10%B to 60%B in 20 min.
Flow rate: 1.0 mL / min
Sample: Teicoplanin+Impurity
Temperature: 35 ,
Separation by Optimix C18/Amide
Bring extremely polar and non-polar analytes closer to shorten the analysis time under isocratic conditions
t=2.023 min
t= 15.278 min
t=1.658min
t=57.216min
Mobile Phase A: Water, B: Methanol
Sample: Uracil, Toluene
Flow rate: 1mL/min
Detection: UV 254nm
t0= 1.52 min
Column: Optimix C18/amide,5μm, 4.6*150mm
Mobile Phase: A : B = 50:50
Column: C18, 5μm, 4.6*150mm
Mobile Phase: A : B= 60 : 40
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Solutions for Bio-molecules
Agela Technologies offers a broad line of HPLC columns for Bio-molecules, including reverse phase, normal phase, ion-exchange, HILIC and size
exclusion columns. All column packing materials are made of ultra pure silica, bonded with pure silanes to ensure the surface inertness.
General guide for the column selection:
1. Small peptide: C18, C8, C4; 100Å or 150 Å
2. Large peptide and proteins: C8, C4, 300 Å; ion-exchange; HILIC; size exclusion
3. Mono- and oligo-saccharides: ion-exchange; NH2; HILIC
4. Polysaccharides: ion-exchange; size exclusion
5. Oligo-nuclei: ion-exchange; reverse phase
6. Nuclei acids: ion-exchange; size exclusion
See the section of “ordering information by the type of stationary phase” for ordering or call our technical staff for helps in selecting the
columns you need.
Solutions for Preparative HPLC (See Purification Section for More Information)
Agela Technologies offers a full line of preparative HPLC columns to meet a variety of application needs.
Our preparative columns feature:
1. Great scalability
2. Excellent bed stability
3. High loading capacity
4. Broad solvent compatibility from Unisol C18, Unisol Amide and Venusil ASB-C18 (100% aqueous to 100% organic solvents)
5. Broad pH range (1-12) from Venusil Durashell
6. Unique selectivity from Unisol Amide and Venusil ASB-C18
Unisol-Amide: an unique phase from Agela Technologies that offers a good alternative selectivity to reverse phase columns and an excellent solu-
tion for purifying highly polar compounds.
Durashell RP: a high pH tolerance enable the use of high pH mobile phase, which results in great improvement of peak shape and loadability of
basic compounds.
Solutions for SFC
Agela Technologies offers a broad line of normal phase columns of different selectivity for SFC applications, including:
Unisol Amide
Venusil NH2
Venusil Silica
Venusil CN
Venusil Nitrophenyl
They are all made of high purity silica materials and are packed using our robust and reproducible process to ensure the high efficiency and the
extended lifetime.
See the section of “Ordering Information by the Type of Stationary Phase” for ordering or call our technical staff for helps.
Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556
HP
LC C
olum
ns
044
AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
Ordering Information by the Type of Stationary Phases
Reverse Phase Columns
Reverse Phase Columns for Small MoleculesAnalytical Columns, Particle Size: 5µm
Promosil C18
General C18,
100Å, 320
m2/g; C%=10
PM950202-0
PM950302-0
PM950502-0
PM951002-0
PM951505-0
PM950303-0
PM950503-0
PM951003-0
PM951503-0
PM950505-0
PM951005-0
PM951505-0
PM952005-0
PM952505-0
PM950105-0
CH-100
Dimension(mm)
2.1×20
2.1×30
2.1×50
2.1×100
2.1×150
3.0x30
3.0x50
3.0x100
3.0x150
4.6×50
4.6×100
4.6×150
4.6×200
4.6×250
4.6×10
ID: 2.1, 3.0, 4.6
PromosilC8
General C8,
100Å, 320
m2/g; C%=10
PM850202-0
PM850302-0
PM850502-0
PM851002-0
PM851505-0
PM850303-0
PM850503-0
PM851003-0
PM851503-0
PM850505-0
PM851005-0
PM851505-0
PM852005-0
PM852505-0
PM850105-0
CH-100
PromosilCN
100Å, 320
m2/g, C%=5
PM550202-0
PM550302-0
PM550502-0
PM551002-0
PM551505-0
PM550303-0
PM550503-0
PM551003-0
PM551503-0
PM550505-0
PM551005-0
PM551505-0
PM552005-0
PM552505-0
PM550105-0
CH-100
Venusil XBP Phenyl
100Å, 380
m2/g, C%=12
VX650202-0
VX650302-0
VX650502-0
VX651002-0
VX651502-0
VX650303-0
VX650503-0
VX651003-0
VX651503-0
VX650505-0
VX651005-0
VX651505-0
VX652005-0
VX652505-0
VX650105-0
CH-100
Venusil XBP C4
100Å, 380
m2/g, C%=7
VX450202-0
VX450302-0
VX450502-0
VX451002-0
VX451502-0
VX450303-0
VX450503-0
VX451003-0
VX451503-0
VX450505-0
VX451005-0
VX451505-0
VX452005-0
VX452505-0
VX450105-0
CH-100
Venusil XBP C1
100Å, 380
m2/g; C%= 3
VX150202-0
VX150302-0
VX150502-0
VX151002-0
VX151502-0
VX150303-0
VX150503-0
VX151003-0
VX151503-0
VX150505-0
VX151005-0
VX151505-0
VX152005-0
VX152505-0
VX150105-0
CH-100
Venusil XBP CN
100Å, 380
m2/g, C%=7
VC950202-0
VC950302-0
VC950502-0
VC951002-0
VC951502-0
VC950303-0
VC950503-0
VC951003-0
VC951503-0
VC950505-0
VC951005-0
VC951505-0
VC952005-0
VC952505-0
VC950105-0
CH-100
ultra surface inertness and reproducibility; balanced retention;
100Å, 380 m2/g, C%=19
same as XBP C18 with larger pore size;
150Å, 200 m2/g; C%=15
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Guard Cartridge
Cartridge Holder
Dimension(mm)
Column Type
2.1×30
2.1×50
2.1×100
2.1×150
3.0x30
3.0x50
3.0x100
3.0x150
4.6×50
4.6×100
4.6×150
4.6×200
4.6×250
4.6×10, 4/pk
20x10
5
5
5
5
5
5
5
5
5
5
5
5
5
5
VX950302-2
VX950502-2
VX951002-2
VX951502-2
VX950303-2
VX950503-2
VX951003-2
VX951503-2
VX950505-2
VX951005-2
VX951505-2
VX952005-2VX952505-2
VX950105-2
CH-200
VX950302-L
VX950502-L
VX951002-L
VX951502-L
VX950303-L
VX950503-L
VX951003-L
VX951503-L
VX950505-L
VX951005-L
VX951505-L
VX952005-L
VX952505-L
VX950105-L
CH-200
Venusil XBP C18 (2)
Venusil XBP C18 (L)
Particle Size(µm)
HP
LC C
olum
ns
045
Best ValueGuaranteed Product QualityInnovation to Benefit Customers
Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556
2.1×30
2.1×50
2.1×100
2.1×150
3.0x30
3.0x50
3.0x100
3.0x150
4.6×50
4.6×100
4.6×150
4.6×200
4.6×250
G 4.6×10, 4/pk
2.1×20
2.1×30
2.1×50
2.1×100
2.1×150
3.0x30
3.0x50
3.0x100
3.0x150
G 3.0×10
4.6×50
4.6×100
4.6×150
G4.6×10
GCH 2.1, 3.0, 4.6
10×150
10×250
20×50
20×150
20×250
30×100
30×150
30×250
G 20×10
10×150
10×250
20×50
20×150
20×250
30×100
30×150
30×250
50×150
50×250
G 20×10
GCH 20x10
VS950302-0
VS950502-0
VS951002-0
VS951502-0
VS950303-0
VS950503-0
VS951003-0
VS951503-0
VS950505-0
VS951005-0
VS951505-0
VS952005-0
VS952505-0
VS950105-0
VS930202-0
VS930302-0
VS930502-0
VS931002-0
VS931502-0
VS930303-0
VS930503-0
VS931003-0
VS931503-0
VS930103-0
VS930505-0
VS931005-0
VS931505-0
VS930105-0
CH-100
VS951510-0
VS952510-0
VS950520-0
VS951520-0
VS952520-0
VS951030-0
VS951530-0
VS952530-0
VS950120-0
VS901510-0
VS902510-0
VS900520-0
VS901520-0
VS902520-0
VS901030-0
VS901530-0
VS902530-0
VS901550-0
VS902550-0
VS90120-0
CH-200
VS850302-0
VS850502-0
VS851002-0
VS851502-0
VS850303-0
VS850503-0
VS851003-0
VS851503-0
VS850505-0
VS851005-0
VS851505-0
VS852005-0
VS852505-0
VS850105-0
VS830202-0
VS830302-0
VS830502-0
VS831002-0
VS831502-0
VS830303-0
VS830503-0
VS831003-0
VS831503-0
VS830103-0
VS830502-0
VS831002-0
VS831505-0
VS830105-0
CH-200
VA950302-0
VA950502-0
VA951002-0
VA951502-0
VA950303-0
VA950503-0
VA951003-0
VA951503-0
VA950505-0
VA951005-0
VA951505-0
VA952005-0
VA952505-0
VA950105-0
VA930202-0
VA930302-0
VA930502-0
VA931002-0
VA931502-0
VA930303-0
VA930503-0
VA931003-0
VA931503-0
VA930103-0
VA930505-0
VA930505-0
VA931505-0
VA930105-0
CH-100
VA951510-0
VA952510-0
VA950520-0
VA951520-0
VA952520-0
VA951030-0
VA951530-0
VA952530-0
VA950120-0
CH-200
UO950302-0
UO950502-0
UO951002-0
UO951502-0
UO950303-0
UO950503-0
UO951003-0
UO951503-0
UO950505-0
UO951005-0
UO951505-0
UO952005-1
UO952505-0
UO950105-0
UO930202-0
UO930302-0
UO930502-0
UO931002-0
UO931502-0
UO930303-0
UO930503-0
UO931003-0
UO931503-0
UO930103-0
UO930505-0
UO931005-0
UO931505-0
UO930105-0
CH-100
UO951510-0
UO952510-0
UO950520-0
UO951520-0
UO952520-0
UO951030-0
UO951530-0
UO952530-0
UO950120-0
UO901510-0
UO902510-0
UO900520-0
UO901520-0
UO902520-0
UO901030-0
UO901530-0
UO902530-0
UO901550-0
UO902550-0
UO900120-0
CH-200
DS950302-0
DS950502-0
DS951002-0
DS951502-0
DS950303-0
DS950503-0
DS951003-0
DS951503-0
DS950505-0
DS951005-0
DS951505-0
DS952005-0
DS952505-0
DS950105-0
DS930202-0
DS930302-0
DS930502-0
DS931002-0
DS931502-0
DS930303-0
DS930503-0
DS931003-0
DS931503-0
DS930103-0
DS930505-0
DS931005-0
DS931505-0
DS930105-0
CH-100
DS951510-0
DS952510-0
DS950520-0
DS951520-0
DS952520-0
DS951030-0
DS951530-0
DS952530-0
DS950120-0
DS901510-0
DS902510-0
DS900520-0
DS901520-0
DS902520-0
DS901030-0
DS901530-0
DS902530-0
DS901550-0
DS902550-0
DS900120-0
CH-200
VX950302-0
VX950502-0
VX951002-0
VX951502-0
VX950303-0
VX950503-0
VX951003-0
VX951503-0
VX950505-0
VX951005-0
VX951505-0
VX952005-0
VX952505-0
VX950105-0
VX930202-0
VX930302-0
VX930502-0
VX931002-0
VX931502-0
VX930303-0
VX930503-0
VX931003-0
VX931503-0
VX930103-0
VX930505-0
VX931005-0
VX931505-0
VX930105-0
CH-100
VX951510-0
VX952510-0
VX950520-0
VX951520-0
VX952520-0
VX951030-0
VX951530-0
VX952530-0
VX950120-0
VX901510-0
VX902510-0
VX900520-0
VX901520-0
VX902520-0
VX901030-0
VX901530-0
VX902530-0
VX901550-0
VX902550-0
VX900120-0
CH-200
VX850302-0
VX850502-0
VX851002-0
VX851502-0
VX850303-0
VX850503-0
VX851003-0
VX851503-0
VX850505-0
VX851005-0
VX851505-0
VX852005-0
VX852505-0
VX850105-0
VX830202-0
VX830302-0
VX830502-0
VX831002-0
VX831502-0
VX830303-0
VX830503-0
VX831003-0
VX831503-0
VX830103-0
VX830502-0
VX831002-0
VX831505-0
VX830105-0
CH-100
VX851510-0
VX852510-0
VX850520-0
VX851520-0
VX852520-0
VX851030-0
VX851530-0
VX852530-0
VX850120-0
VX801510-0
VX802510-0
VX800520-0
VX801520-0
VX802520-0
VX801030-0
VX801530-0
VX802530-0
VX801550-0
VX802550-0
VX800120-0
CH-200
5
5
5
5
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
3
3
3
3
3
3
3
5
5
5
5
5
5
5
5
5
10
10
10
10
10
10
10
10
10
10
10
Venusil ASB C18
Venusil ASB C8
Venusil AQ C18
Unisol C18
Durashell RP
Venusil XBP C18
Venusil XBP C8
Particle Size(µm)
Dimension(mm)
G=Guard Cartridge; GCH= Guard Cartridge Holder
C8 for low pH and low bleed; 150 Å, 200m2/g, C%=7
extreme low pH tolerance (0.8-7.5), low bleed; 150 Å, 200m2/g; C%=12
for high aqueous applications; compatible with 100% water; 100Å, 380 m2/g, C%=18
universal RP; compatible with 100% water; 100Å, 410 m2/g, C%=17
high pH tolerance (1.5-12); 150Å,240 m2/g, C%=15
highest hydro-phobicity;100Å, 380m2/g, C%=22
general RP;100Å, 380 m2/g; C%= 14
HP
LC C
olum
ns
046
AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
Particle Size: 5µm; Pore Size: 300Å; Surface Area: 45 m2/g
2.1×30
2.1×50
2.1×100
2.1×150
3.0x30
3.0x50
3.0x100
3.0x150
4.6×50
4.6×100
4.6×150
4.6×200
4.6×250
4.6×10, 4/pk
ID: 2.1, 3.0, 4.6
10×150
10×250
20×50
20×150
20×250
20×10
20×10
Type Dimension (mm) Venusil ASB C18 Venusil ASB C8 Venusil XBP C18 Venusil XBP C8
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Guard Cartridge
Cartridge Holder
Semi-Prep
Semi-Prep
Semi-Prep
Semi-Prep
Semi-Prep
Guard Cartridge
Cartridge Holder
extreme low pH
tolerance (0.8-7.5),
low bleed
VS950302-T
VS950502-T
VS951002-T
VS951502-T
VS950303-T
VS950503-T
VS951003-T
VS951503-T
VS950505-T
VS951005-T
VS951505-T
VS952005-T
VS952505-T
VS950105-T
CH-100
VS951510-T
VS952510-T
VS950520-T
VS951520-T
VS952520-T
VS950120-T
CH-200
C8 for low pH
and low bleed
VS850302-T
VS850502-T
VS851002-T
VS851502-T
VS850303-T
VS850503-T
VS851003-T
VS851503-T
VS850505-T
VS851005-T
VS851505-T
VS852005-T
VS852505-T
VS850105-T
CH-100
VS851510-T
VS852510-T
VS850520-T
VS851520-T
VS852520-T
VS850120-T
CH-200
highest
hydrophobicity
VX950302-T
VX950502-T
VX951002-T
VX951502-T
VX950303-T
VX950503-T
VX951003-T
VX951503-T
VX950505-T
VX951005-T
VX951505-T
VX952005-T
VX952505-T
VX950105-T
CH-100
VX951510-T
VX952510-T
VX950520-T
VX951520-T
VX952520-T
VX950120-T
CH-200
General RP
VX850302-T
VX850502-T
VX851002-T
VX851502-T
VX850303-T
VX850503-T
VX851003-T
VX851503-T
VX850505-T
VX851005-T
VX851505-T
VX852005-T
VX852505-T
VX850105-T
CH-100
VX851510-T
VX852510-T
VX850520-T
VX851520-T
VX852520-T
VX850120-T
CH-200
Reverse Phase Columns for Large MoleculesParticle Size: 5µm; Pore Size: 300Å; Surface Area: 45 m2/g
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Guard Cartridge
Cartridge Holder
Semi-prep
Semi-prep
Semi-prep
Semi-prep
Semi-prep
Guard Cartridge
Cartridge Holder
2.1×30
2.1×50
2.1×100
2.1×150
3.0x30
3.0x50
3.0x100
3.0x150
4.6×50
4.6×100
4.6×150
4.6×200
4.6×250
4.6×10, 4/pk
2.1, 3.0, 4.6
10×150
10×250
20×50
20×150
20×250
20×10
20×10
VX650302-T
VX650502-T
VX651002-T
VX651502-T
VX650303-T
VX650503-T
VX651003-T
VX651503-T
VX650505-T
VX651005-T
VX651505-T
VX652005-T
VX652505-T
VX650105-T
CH-100
VX651510-T
VX652510-T
VX650520-T
VX651520-T
VX652520-T
VX650120-T
CH-200
VX450302-T
VX450502-T
VX451002-T
VX451502-T
VX450303-T
VX450503-T
VX451003-T
VX451503-T
VX450505-T
VX451005-T
VX451505-T
VX452005-T
VX452505-T
VX450105-T
CH-100
VX451510-T
VX452510-T
VX450520-T
VX451520-T
VX452520-T
VX450120-T
CH-200
VX150302-T
VX150502-T
VX151002-T
VX151502-T
VX150303-T
VX150503-T
VX151003-T
VX151503-T
VX150505-T
VX151005-T
VX151505-T
VX152005-T
VX152505-T
VX150105-T
CH-100
VX151510-T
VX152510-T
VX150520-T
VX151520-T
VX152520-T
VX150120-T
CH-200
VC950302-T
VC950502-T
VC951002-T
VC951502-T
VC950303-T
VC950503-T
VC951003-T
VC951503-T
VC950505-T
VC951005-T
VC951505-T
VC952005-T
VC952505-T
VC950105-T
CH-100
VC951510-T
VC952510-T
VC950520-T
VC951520-T
VC952520-T
VC950120-T
CH-200
Column Type Dimension (mm) Venusil XBP Phenyl Venusil XBP C4 Venusil XBP C1 Venusil XBP CN
HP
LC C
olum
ns
047
Best ValueGuaranteed Product QualityInnovation to Benefit Customers
Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556
Normal Phase Columns
Normal Phase Columns for Small MoleculesParticle Size: 5µm
Dimension (mm)
2.1×30
2.1×50
2.1×100
2.1×150
3.0x30
3.0x50
3.0x100
3.0x150
4.6×50
4.6×100
4.6×150
4.6×200
4.6×250
4.6×10 , 4/pk
2.1,3.0,4.6
10×150
10×250
20×50
20×150
20×250
30×100
30×150
30×250
20×10
10×150
10×250
20×50
20×150
20×250
30×100
30×150
30×250
50×150
50×250
20×10
2.1,3.0,4.6
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
10
10
10
10
10
10
10
10
10
10
10
Type
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Guard cartridge,
GCH
Semi-preparative
Semi-preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Guard Cartridge
Semi-preparative
Semi-preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Guard Cartridge
GCH
Particle Size (µm)
Unisol Amide (HILIC)
Venusil Silica (HILIC II)
Venusil XBP-NH2
(HILIC III)Venusil XBP-
DiolVenusil XBP
CN
unique selectivity;
100Å, 410 m2/g,
VH950302-0
VH950502-0
VH951002-0
VH951502-0
VH950303-0
VH950503-0
VH951003-0
VH951503-0
VH950505-0
VH951005-0
VH951505-0
VH952005-0
VH952505-0
VH950105-0
CH-100
VH951510-0
VH952510-0
VH950520-0
VH951520-0
VH952520-0
VH951030-0
VH951530-0
VH952530-0
VH950220-0
VH901510-0
VH902510-0
VH900520-0
VH901520-0
VH902520-0
VH901030-0
VH901530-0
VH902530-0
VH901550-0
VH902550-0
VH900120-0
CH-200
ultra pure ;
100Å, 380m2/g,
VSi950302-0
VSi950502-0
VSi951002-0
VSi951502-0
VSi950303-0
VSi950503-0
VSi951003-0
VSi951503-0
VSi950505-0
VSi951005-0
VSi951505-0
VSi952005-0
VSi952505-0
VSi950105-0
CH-100
VSi951510-0
VSi952510-0
VSi950520-0
VSi951520-0
VSi952520-0
VSi951030-0
VSi951530-0
VSi952530-0
VSi950220-0
VSi901510-0
VSi902510-0
VSi900520-0
VSi901520-0
VSi902520-0
VSi901030-0
VSi901530-0
VSi902530-0
VSi901550-0
VSi902550-0
VSi900120-0
CH-200
ultra pure; 100 Å,
380m2/g;
VN850302-0
VN850502-0
VN851002-0
VN851502-0
VN850303-0
VN850503-0
VN851003-0
VN851503-0
VN850505-0
VN851005-0
VN851505-0
VN852005-0
VN852505-0
VN850105-0
CH-100
VN851510-0
VN852510-0
VN850520-0
VN851520-0
VN852520-0
VN851030-0
VN851530-0
VN852530-0
VN850220-0
VN801510-0
VN802510-0
VN800520-0
VN801520-0
VN802520-0
VN801030-0
VN801530-0
VN802530-0
VN801550-0
VN802550-0
VN800120-0
CH-200
ultra pure; 100Å,
380 m2/g,
VC950302-0
VC950502-0
VC951002-0
VC951502-0
VC950303-0
VC950503-0
VC951003-0
VC951503-0
VC950505-0
VC951005-0
VC951505-0
VC952005-0
VC952505-0
VC950105-0
CH-100
VC951510-0
VC952510-0
VC950520-0
VC951520-0
VC952520-0
VC951030-0
VC951530-0
VC952530-0
VC950220-0
VC901510-0
VC902510-0
VC900520-0
VC901520-0
VC902520-0
VC901030-0
VC901530-0
VC902530-0
VC901550-0
VC902550-0
VC900120--0
CH-200
ultra pure; 100Å,
380 m2/g;
VD950302-0
VD950502-0
VD951002-0
VD951502-0
VD950303-0
VD950503-0
VD951003-0
VD951503-0
VD950505-0
VD951005-0
VD951505-0
VD952005-0
VD952505-0
VD950105-0
CH-100
GCH=Guard Cartridge Holder
HP
LC C
olum
ns
048
AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
2.1×30
2.1×50
2.1×100
2.1×150
3.0x30
3.0x50
3.0x100
3.0x150
4.6×50
4.6×100
4.6×150
4.6×200
4.6×250
4.6×10 , 4/pk
2.1,3.0,4.6
10×150
10×250
20×50
20×150
20×250
30×100
30×150
30×250
20×20
Type Dimension (mm) Particle Size (µm) Promosil -CN Promosil-Silica Promosil -NH2
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Guard cartridge,
Guard cartridge Holder
Semi-preparative
Semi-preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Guard Cartridge
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
PM550302-0
PM550502-0
PM551002-0
PM551502-0
PM550303-0
PM550503-0
PM551003-0
PM551503-0
PM550505-0
PM551005-0
PM551505-0
PM552005-0
PM552505-0
PM550105-0
CH-100
PM050302-0
PM050502-0
PM051002-0
PM051502-0
PM050303-0
PM050503-0
PM051003-0
PM051503-0
PM050505-0
PM051005-0
PM051505-0
PM052005-0
PM052505-0
PM050105-0
CH-100
PM051510-0
PM052510-0
PM050520-0
PM051520-0
PM052520-0
PM051030-0
PM051530-0
PM052530-0
PM050220-0
PM750302-0
PM750502-0
PM751002-0
PM751505-0
PM750303-0
PM750503-0
PM751003-0
PM751503-0
PM750505-0
PM751005-0
PM751505-0
PM752005-0
PM752505-0
PM750105-0
CH-100
High Purity; Pore Size: 100 Å; Surface Aera: 320m2/g
HP
LC C
olum
ns
049
Best ValueGuaranteed Product QualityInnovation to Benefit Customers
Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556
Normal Phase Columns for Large Molecules
Ion-exchange Columns
Ultra Pure Silica; Pore Size:300 Å; Surface Area:45m2/g
Dimension (mm)
2.1×30
2.1×50
2.1×100
2.1×150
3.0x30
3.0x50
3.0x100
3.0x150
4.6×50
4.6×100
4.6×150
4.6×200
4.6×250
4.6×10, 4/pk
ID: 2.1, 3.0, 4.6
10×150
10×250
20×50
20×150
20×250
30×100
30×150
30×250
20×10
20x10
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
Type
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Guard Cartridge
GCH
Semi-preparative
Semi-preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Guard Cartridge
GCH
Particle Size (µm)
Venusil XBP NH
2
Venusil XBP Diol
Venusil XBP CN
Unisol Amide (HILIC)
Venusil XBPSilica
VN850302-T
VN850502-T
VN851002-T
VN851502-T
VN850303-T
VN850503-T
VN851003-T
VN851503-T
VN850505-T
VN851005-T
VN851505-T
VN852005-T
VN852505-T
VN850105-T
CH-100
VN851510-T
VN852510-T
VN850520-T
VN851520-T
VN852520-T
VN851030-T
VN851530-T
VN852530-T
VN850120-T
CH-200
VD950302-T
VD950502-T
VD951002-T
VD951502-T
VD950303-T
VD950503-T
VD951003-T
VD951503-T
VD950505-T
VD951005-T
VD951505-T
VD952005-T
VD952505-T
VD950105-T
CH-100
VC950302-T
VC950502-T
VC951002-T
VC951502-T
VC950303-T
VC950503-T
VC951003-T
VC951503-T
VC950505-T
VC951005-T
VC951505-T
VC952005-T
VC952505-T
VC950105-T
CH-100
VC951510-T
VC952510-T
VC950520-T
VC951520-T
VC952520-T
VC951030-T
VC951530-T
VC952530-T
VC950120-T
CH-200
VSi950302-T
VSi950502-T
VSi951002-T
VSi951502-T
VSi950303-T
VSi950503-T
VSi951003-T
VSi951503-T
VSi950505-T
VSi951005-T
VSi951505-T
VSi952005-T
VSi952505-T
VSi950105-T
CH-100
VSi951510-T
VSi952510-T
VSi950520-T
VSi951520-T
VSi952520-T
VSi951030-T
VSi951530-T
VSi952530-T
VSi950120-T
CH-200
VH950302-T
VH950502-T
VH951002-T
VH951502-T
VH950303-T
VH950503-T
VH951003-T
VH951503-T
VH950505-T
VH951005-T
VH951505-T
VH952005-T
VH952505-T
VH950105-T
CH-100
Surface Aera: 200m2/g Pore Size: 150Å
Dimension(mm)
Particle Size(μm)
VenusilSCX
Type
Analytical
Analytical
Analytical
Guard cartridge
4.6×100
4.6×150
4.6×250
4.6×10 4/pk
5
5
5
5
VSc951005-0
VSc951505-0
VSc952505-0
VSc950105-0
Surface Aera: 50m2/g Pore Size: 300Å
Dimension(mm)
Particle Size(μm)
VenusilSCX
Type
Analytical
Analytical
Analytical
Guard cartridge
4.6×100
4.6×150
4.6×250
4.6×10 4/pk
5
5
5
5
VSc951005-T
VSc951505-T
VSc952505-T
VSc950105-T
VenusilSAX
VSa951005-0
VSa951505-0
VSa952505-0
VSa950105-0
VenusilSAX
VSa951005-T
VSa951505-T
VSa952505-T
VSa950105-T
GCH=Guard cartridge Holder
HP
LC
Colu
mns
050
AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
Mixed Phase Columns
Type Dimension (mm) Particle Size(µm) Optimix C18/C8 Optimix C18/amide Optimix C18/phenyl Optimix C18/CN
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Guard cartridge
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Guard cartridge
2.1×30
2.1×50
2.1×100
2.1×150
4.6×50
4.6×100
4.6×150
4.6×200
4.6×250
4.6×10
2.1×30
2.1×50
2.1×100
2.1×150
4.6×50
4.6×100
4.6×150
4.6×10
OP950302-OC
OP950502-OC
OP951002-OC
OP951502-OC
OP950505-OC
OP951005-OC
OP951505-OC
OP952005-OC
OP952505-OC
OP950105-OC
OP930302-OC
OP930502-OC
OP931002-OC
OP931502-OC
OP930505-OC
OP931005-OC
OP931505-OC
OP930105-OC
OP950302-AM
OP950502-AM
OP951002-AM
OP951502-AM
OP950505-AM
OP951005-AM
OP951505-AM
OP952005-AM
OP952505-AM
OP950105-AM
OP930302-AM
OP930502-AM
OP931002-AM
OP931502-AM
OP930505-AM
OP931005-AM
OP931505-AM
OP930105-AM
OP950302-PH
OP950502-PH
OP951002-PH
OP951502-PH
OP950505-PH
OP951005-PH
OP951505-PH
OP952005-PH
OP952505-PH
OP950105-PH
OP930302-PH
OP930502-PH
OP931002-PH
OP931502-PH
OP930505-PH
OP931005-PH
OP931505-PH
OP930105-PH
OP950302-CN
OP950502-CN
OP951002-CN
OP951502-CN
OP950505-CN
OP951005-CN
OP951505-CN
OP952005-CN
OP952505-CN
OP950105-CN
OP930302-CN
OP930502-CN
OP931002-CN
OP931502-CN
OP930505-CN
OP931005-CN
OP931505-CN
OP930105-CN
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
3
Type Dimension (mm) Particle Size(µm) Optimix C18/SCX Optimix C18/SAX Optimix C18/Nitrophenyl
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Analytical
Guard cartridge
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Fast analysis
Guard cartridge
2.1×30
2.1×50
2.1×100
2.1×150
4.6×50
4.6×100
4.6×150
4.6×200
4.6×250
4.6×10
2.1×30
2.1×50
2.1×100
2.1×150
4.6×50
4.6×100
4.6×150
4.6×10
OP950302-SC
OP950502-SC
OP951002-SC
OP951502-SC
OP950505-SC
OP951005-SC
OP951505-SC
OP952005-SC
OP952505-SC
OP950105-SC
OP930302-SC
OP930502-SC
OP931002-SC
OP931502-SC
OP930505-SC
OP931005-SC
OP931505-SC
OP930105-SC
OP950302-SA
OP950502-SA
OP951002-SA
OP951502-SA
OP950505-SA
OP951005-SA
OP951505-SA
OP952005-SA
OP952505-SA
OP950105-SA
OP930302-SA
OP930502-SA
OP931002-SA
OP931502-SA
OP930505-SA
OP931005-SA
OP931505-SA
OP930105-SA
OP950302-NP
OP950502-NP
OP951002-NP
OP951502-NP
OP950505-NP
OP951005-NP
OP951505-NP
OP952005-NP
OP952505-NP
OP950105-NP
OP930302-NP
OP930502-NP
OP931002-NP
OP931502-NP
OP930505-NP
OP931005-NP
OP931505-NP
OP930105-NP
5
5
5
5
5
5
5
5
5
5
3
3
3
3
3
3
3
3
HP
LC C
olum
ns
051
Best ValueGuaranteed Product QualityInnovation to Benefit Customers
Ordering Phone No.: (302) 438 8798Support Phone No.: (302) 588 4556
Size Exclusion Columns (Silica Based)
Polymer Phase Columns
Dimension (mm)
4.6x 150
4.6x 250
4.6x 150
4.6x 250
10x150
10x250
10x150
10x250
5
5
10
10
5
5
10
10
Type
Analytical
Analytical
Analytical
Analytical
Semi-prep.
Semi-prep.
Semi-prep.
Semi-prep.
Particle Size (µm)
GF GF 300 GF amide GFamide-300
ultra pure,high porosity;
bonded 120Å,
GF151505-0
GF152505-0
GF101505-0
GF102505-0
GF151510-0
GF152510-0
GF101510-0
GF102510-0
ultra pure,high porosity;
bonded 300Å,
GF351505-0
GF352505-0
GF301505-0
GF302505-0
GF351510-0
GF352510-0
GF301510-0
GF302510-0
ultra pure,high porosity;
bonded with amide 120Å,
GF151505-A
GF152505-A
GF101505-A
GF102505-A
GF151510-A
GF152510-A
GF101510-A
GF102510-A
ultra pure,high porosity;
bonded with amide 300Å,
GF301505-A
GF302505-A
GF301505-A
GF302505-A
GF301510-A
GF302510-A
GF301510-A
GF302510-A
RPC-Analytical Prepacked Columns
Dimension(mm)
Particle(μm)Matrix
AKF-RP-M-154607
AKF-RP-M-154610
AKF-RP-M-154620
AKF-RP-H-254605
AKF-RP-H-254607
AKF-RP-H-254610
AKF-RP-H-254620
4.6 150
4.6 150
4.6 150
4.6 250
4.6 250
4.6 250
4.6 250
7
10
20
5
7
10
20
RPC-Preparative Prepacked Columns
Dimension(mm)
Particle(μm)Matrix
AKF-RP-M-052007
AKF-RP-M-102007
AKF-RP-M-251007
AKF-RP-M-252007
AKF-RP-M-151010
AKF-RP-M-152010
AKF-RP-H-151010
AKF-RP-H-152010
AKF-RP-M-251010
AKF-RP-M-252010
AKF-RP-H-251010
AKF-RP-H-252010
AKF-RP-H-152020
AKF-RP-H-252020
AKF-RP-H-105020
AKF-RP-H-255020
AKF-RP-H-2510020
20 50
20 100
10 250
20 250
10 150
20 150
10 150
20 150
10 250
20 250
10 250
20 250
20 150
20 250
50 100
50 250
100 250
5
5
7
7
10
10
10
10
10
10
10
10
20
20
20
20
20
HP
LC
Colu
mns
052
AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
Ion Exchange Chromatography Columns (strong cation)
Dimension(mm)
Particle(μm)Matrix
AKF-CISLM-050207
AKF-CISLM-150207
AKF-CISLM-154607
AKF-CISLM-154610
AKF-CISLM-154620
AKF-CISLH-154607
AKF-CISLH-154610
AKF-CISLH-154620
AKF-CISLM-254610
AKF-CISLH-254610
AKF-CISLH-251010
AKF-CISLH-101010
2.0 50
2.0 150
4.6 150
4.6 150
4.6 150
4.6 150
4.6 150
4.6 150
4.6 250
4.6 250
10 250
10 100
5
5
7
10
20
7
10
20
10
10
10
10
Hydrophobic Interaction Chromatography Columns
Dimension(mm)
Particle(μm)Matrix
AKF-PHEMM-154607
AKF-PHEMM-154610
AKF-PHEMM-154620
AKF-PHEMH-254607
AKF-PHEMH-254610
AKF-PHEMH-254620
AKF-PHEMH-036410
AKF-C4-MM-154607
AKF-C4-MM-154610
AKF-C4-MM-154620
AKF-C4-MH-254607
AKF-C4-MH-254610
AKF-C4-MH-254620
AKF-C4-MH-036410
AKF-C8-MM-154607
AKF-C8-MM-154610
AKF-C8-MM-154620
AKF-C8-MH-254607
AKF-C8-MH-254610
AKF-C8-MH-254620
AKF-C8-MH-036410
AKF-ETHMM-154607
AKF-ETHMM-154610
AKF-ETHMM-154620
AKF-ETHMH-254607
AKF-ETHMH-254610
AKF-ETHMH-254620
AKF-ETHMH-036410
4.6 150
4.6 150
4.6 150
4.6 250
4.6 250
4.6 250
6.4 30
4.6 150
4.6 150
4.6 150
4.6 250
4.6 250
4.6 250
6.4 30
4.6 150
4.6 150
4.6 150
4.6 250
4.6 250
4.6 250
6.4 30
4.6 150
4.6 150
4.6 150
4.6 250
4.6 250
4.6 250
6.4 30
7
10
20
7
10
20
10
7
10
20
7
10
20
10
7
10
20
7
10
20
10
7
10
20
7
10
20
10
HP
LC
Colu
mns
053
Best Value
Guaranteed Product Quality
Innovation to Benefit Customers
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
Ion Exchange Chromatography Columns (Weak cation)
Dimension(mm)
Particle(μm)Matrix
AKF-CLV-050207
AKF-CLV-150207
AKF-CLV-154607
AKF-CLV-154610
AKF-CLV-154620
AKF-CHV-154607
AKF-CHV-154610
AKF-CHV-154620
AKF-CLV-254610
AKF-CHV-254610
AKF-CHV-251010
AKF-CHV-101010
2.0 50
2.0 150
4.6 150
4.6 150
4.6 150
4.6 150
4.6 150
4.6 150
4.6 250
4.6 250
10 250
10 100
5
5
7
10
20
7
10
20
10
10
10
10
Ion Exchange Chromatography Columns (Strong Anion)
Dimension(mm)
Particle(μm)Matrix
AKF-QLV-050207
AKF-QLV-150207
AKF-QLV-154607
AKF-QLV-154610
AKF-QLV-154620
AKF-QHV-154607
AKF-QHV-154610
AKF-QHV-154620
AKF-QLV-254610
AKF-QHV-254610
AKF-QHV-251010
AKF-QHV-101010
2.0 50
2.0 150
4.6 150
4.6 150
4.6 150
4.6 150
4.6 150
4.6 150
4.6 250
4.6 250
10 250
10 100
5
5
7
10
20
7
10
20
10
10
10
10
Ion Exchange Chromatography Columns (Weak Anion)
Dimension(mm)
Particle(μm)Matrix
AKF-DEAL-050207
AKF-DEAL-150207
AKF-DEAL-154607
AKF-DEAL-154610
AKF-DEAL-154620
AKF-DEAH-154607
AKF-DEAH-154610
AKF-DEAH-154620
AKF-DEAL-254610
AKF-DEAH-254610
AKF-DEAH-251010
AKF-DEAH-101010
2.0 50
2.0 150
4.6 150
4.6 150
4.6 150
4.6 150
4.6 150
4.6 150
4.6 250
4.6 250
10 250
10 100
5
5
7
10
20
7
10
20
10
10
10
10
HP
LC C
olum
ns
054
AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
Column Selection Parameters
Stationary Phases
• Reverse Phase [C18 (XBP, AQ, ASB), C8 (XBP, ASB),
C4 , Phenyl ]: most HPLC analytical and preparative
separations; use shorter chain if the retention is too
high on C18 columns use shorter chain (C8, C4) for
proteins and larger peptides.
• Normal Phase [Silica, Amino, SAX, SCX]: for those not
applicable on reverse phase; polysaccharides(amino),
ion-exchange chromatography, some preparative needs.
• Bi-mode [Cyano, Venusil HILIC]: can be used in both
reverse and normal phase modes, alternative selectivity
to hydrocarbon-based reverse phases, inert and better
reproducibility than silica columns.
Particle Size
• 3 μm: fast analysis, high throughput analytical
applications, micro/nano HPLC
• 5 μm: analytical and semi-preparative separation
• 10 μm: preparative separation
Pore Size
• Narrow pore (100-150 Å): MW <3000
• Large pore (300-500 Å): 3000< MW <50000
• Mega pore (1000 Å): MW >50000
Column Length
• Short (30 mm, 50 mm): high throughput analysis and
purification, pre-separation
• Regular (100 mm, 150 mm, 250 mm): more complex
sample, larger injection volume
Column Diameter
• Capillary (0.5 mm, 1.0 mm): LC-MS, micro-HPLC, very
small sample volume
• Analytical (2.1 mm, 4.6 mm, 10 mm): standard HPLC,
analytical and mini-prep
• Preparative (21 mm, 30 mm, 50 mm): preparative
HPLC
HPLC Column Selection Guide
Basic Considerations in Choosing HPLC Columns
Analyte
• Molecular Weight – small molecule (<3000), narrow
pore; medium molecule(3000- 50000), large pore; large
molecule(>50000), mega pore.
• Solubility in aqueous solutions – very hydrophilic, use
AQ-C18, ASB-C18 or Venusil HILIC; very hydrophobic,
use shorter chain phases (C8, C4, Phenyl, CN); in
between, use AQ-C18, ASP-C18, XPB-C18 or Phenyl .
• Difference between the compounds to be separated –
by polarity, use AQ C18, ASB-C18, cyano, HILIC; by
shape or regio-isomer, use XBP-C18, phenyl.
Mobile Phase
• Solution solvent: 97-100% aqueous solution, AQ-C18
or ASB-C18; normal phase mode with aqueous mobile
phase, Venusil HILIC
• pH: pH < 2, C18 phases; pH > 9, XBP-C18; pH= 2-9,
most phases; (check the pH range for each column
before use!)
• Salt concentration: high salt concentration >0.1 M
(should be avoided if possible), XBP-C18, SCX, SAX.
Sample
• Analyte mass/concentration: high mass load, larger
diameter column (>10 for preparative).
• Sample volume: small volume, small diameter; large
volume, large diameter and length.
• Sample complexity: simple separation, short column;
complex separation, long column.
Instrument and Application
• Traditional HPLC analysis: 3μm, 5μm; 4.6 x 100 mm,
4.6 x 150 mm, 4.6 x 250 mm
• High throughput analysis: 3μm, 5μm; 2.1 x 30 mm,
2.1 x 50 mm, 4.6 x 50 mm.
• LC-MS application: 3μm, 5μm; 1.0 x 30 mm,
1.0 x 50 mm, 2.1 x 30 mm, 2.1 x 50 mm, 2.1 x 100 mm.
• Micro HPLC: 3μm, 5μm; 0.5 x 30 mm, 0.5 x 50 mm,
0.5 x 100 mm.
• Preparative HPLC: 5μm, 10μm; 21 x 50 mm,
21 x 150 mm, 21 x 250 mm, 30 x 150 mm,
30 x 250 mm, 50 x 250 mm.
HP
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Best Value
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Comparison of Particle Characteristics of Typical HPLC Stationary Phases
Stationary Phase Particle(μm) Pore(A) Surface(m2/g) C% End Capping High Purity Silica
Venusil XBP C18
Venusil AQ C18
Venusil ASB C18
Venusil XBP C18(L)
ZORBAX SB C18
ZORBAX XDB C18
Waters SunFire C18
Waters Symmetry C18
Waters XTerra MS C18
Hypersil BDS C18
Hypersil ODS
Inertsil ODS
Inertsil ODS3
Kromasil C18
Supelco Discovery C18
Luna C18(2)
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
5
100
100
150
150
80
80
100
100
125
130
120
100
100
100
180
100
380
380
200
200
180
180
340
335
—
170
170
350
450
340
200
400
double
yes
no
double
no
yes
yes
yes
yes
yes
yes
yes
yes
yes
yes
yes
yes
yes
yse
yes
yes
yes
yes
yes
—
no
no
no
yes
special
yes
yes
22
18
11
14
10
12
16
19
15.5
11
10
14
15
19
12
17.5
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
Cross-reference for Agela Venusil Series Columns
Agilent
SB C18
SB C8
XDB C18
XDB C8
SB-Aq
Waters
Sunfire C18
Sunfire C8
Atlantis C18 (AQ)
Atlantis HILIC
Phenomenex
Luna C18
Luna C8
Luna C18 (2)
Supelco
Discovery C18
Discovery C8
Asentics C18
Asentics C8
GL Science
Insertsil ODS 3
E: Similar selectivity and superior performance
R: A better replacement
R
E
E
XBP C18 XBP C18 (2)
R
E
E
XBP C8
R
E
XBP(L) C18
R
E
XBP(L) C8
R
ASB C18
R
ASB C8
R
R
E
AQ C18
R
HILIC
R
E
Venusil XBP C18 (2) 5 100 380 19 double yes
HP
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AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
USP Specifications Column Particle Shape
Octadecyl silane bonded to porous silica or ceramic
microparticles, 3 to 10μm in diameter.
Octadecyl silane chemically bonded to silica gel of a controlled surface
porosity that has been bonded to a solid spherical core, 30 to 50μm in diameter
Porous silica microparticles, 5 to 10 μm in diameter
Silica gel of controlled surface porosity bonded to
a solid spherical core, 30 to 50μm in diameter.
Alumina of controlled surface porosity bonded to a
solid spherical core, 30 to 50 μm in diameter.
Strong cation-exchange packing: sulfonated fluorocarbon
polymer coated on a solid spherical core, 30 to 50 μm in diameter.
Octylsilane bonded to totally porous microsilica
particles, 3 to 10 μm in diameter.
An essentially monomolecular layer of aminopropyl-silane chemically
bonded to totally porous silica gel support, 10 μm in diameter.
10 μm irregular totally porous silica gel having a chemically bonded,
strongly acidic cation-exchange coating.
Nitrile groups chemically bonded to porous silica
microparticles, 3 to 10 μm in diameter.
Phenyl groups chemically bonded to porous silica
micro-particles, 3 to 10 μm in diameter.
Strong anion-exchange packing made by chemically bonding a
quaternary amine to a solid silica spherical core, 30 to 50 μm in diameter
Trimethylsilane chemically bonded to porous silica
microparticles, 3 to 10 μm in diameter.
Silica gel, 10 μm in diameter, having a chemically bonded, strongly
basic quaternary ammonium anion-exchange coating.
Hexyl silane chemically bonded to totally porous
silica particles, 3 to 10 μm in diameter.
Dimethyl silane chemically bonded to totally porous
silica particles, 3 to 10 μm in diameter.
USP Column Selection Guide
Strong cation exchange resin consisting of sulfonated cross-linked
styrene-divinylbenzene copolymer in the hydrogen form, 7 to 11 μm in diameter.
Amino and cyano groups chemically bonded to
porous silica particles, 5 to 10 μm in diameter.
Strong cation exchange resin consisting of sulfonated cross-linked
styrene-divinylbenzene copolymer in the calcium form, 9 μm in diameter.
Dihydroxypropane groups chemically bonded to
porous silica particles, 3 to 10 μm in diameter.
L01
L02
L03
L04
L05
L06
L07
L08
L09
L10
L11
L12
L13
L14
L15
L16
L17
L18
L19
L20
Spherical
Spherical
Spherical
Spherical
Irregular
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Irregular
Spherical
Spherical
Venusil C18
Please call
Venusil silica
Please call
Please call
Please call
Venusil C8
Venusil NH2
Please call
Venusil CN
Venusil Phenyl
Please call
Please call
Please call
Please call
Please call
AKF CISLH columns
Please call
AKF CISLM columns
Please call
HP
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Best ValueGuaranteed Product QualityInnovation to Benefit Customers
USP Specifications Column Particle Shape
A rigid, spherical styrene-divinylbenzene copolymer,
5 to 10 μm in diameter.
A cation exchange resin made of porous polystyrene
gel with sulfonic acid groups, about 10 μm in size.
An ion exchange resin made of porous polymethacrylate or polyacrylate
gel with quaternary ammonium groups, about 10 μm in size.
A semi-rigid hydrophilic gel consisting of vinyl polymers with numerous
hydroxyl groups on the matrix surface, 32 to 63 μm in diameter.
Packing having the capacity to separate compounds with a MW range from
100 to 5000 daltons (as determined by polyethylene oxide), applied to neutral
Butyl silane chemically bonded to totally porous
silica particles, 5 to 10 μm in diameter.
Porous silica particles, 30 to 50 μm in diameter.
A multifunctional support, which consists of a high purity, 100 Å spherical
silica substrate that has been bonded with anionic (amine) functionality.
Gamma alumina, reversed phase, low carbon percentage by weight, alumina-based
polybutadiene spherical particles, 5 μm diameter w/a pore diameter of 80 Å
Ethyl silane chemically bonded to a totally porous
silica particle, 3 to 10 μm in diameter.
A strong anion-exchange resin-quaternary amine bonded on latex particles attached
to a core of 8.5 μm macroporous particles having a pore size of 2000 Å.
A chiral ligand-exchange packing- L-proline copper complex covanlently bonded
to irregularly shaped silica particles, 5 to 10 μm in diameter.
Packing having the capacity to separate proteins of 4,000 to 400,000 daltons.
It is spherical, silicabased and processed to provide pH stability.
Strong cation exchange resin consisting of sulfonated cross-linked
styrene-divinylbenzene copolymer in the lead form, 9 μm in diameter.
A zirconium-stabilized spherical silica packing with a hydrophilic (diol-type)
molecular monolayer bonded phase having a pore size of 150 Å.
L-Phenylglycine-3,5-dinitrobenzoyl on 5 μm aminopropyl silica
Polymethacrylate gel for proteins 2000~40,000 MW
Methacrylate-based SEC column for water-solubles
Hydrophilic polyhydroxymethacrylate gel of totally porous spherical resin
Cellulose tris-3,5 dimethylphenylcarbamate coated
porous silica particles, 5 to 20 μm in diameter
L21
L22
L23
L24
L25
L26
L27
L28
L29
L30
L31
L32
L33
L34
L35
L36
L37
L38
L39
L40
Spherical
Spherical
Spherical
Spherical
Spherical
Irregular
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
Spherical
AKF RP columns
AKF CISLH columns
Please call
Please call
Please call
Venusil C4
Please call
Please call
Please call
Please call
Please call
Please call
Please call
Please call
Please call
Nucleosil Chiral-3
Please call
Please call
Please call
Please call
USP Column Selection Guide
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
H
PLC
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AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
Applications
1.Antibiotics
1.1 Benzylpenicillin Sodium
min0 2.5 5 7.5 10 12.5 15
mAU
0
50
100
150
200
250
300Benzylpenicillin Sodium
H O
NHH 2N H
N
S
O
H H
O
C H 3
C H 3
O
O N a
H
Column: Unisol C18 4.6×150mm 5μm
Part No.: UO951505-0
Mobile phase:Phosphate buffer
0.05mol/L ,pH5.0 : ACN
97.5:2.5
Detection: UV 254 nm
Flow rate: 1 mL/min
Injection: 5 μL
Column: Unisol C18 4.6×150mm 5μm
Part No.: UO951505-0
Mobile phase: 0.1mol/L potassium dihydrogen
phosphate buffer
pH 2.5 :ACN 70:30
Detection: UV 225 nm
Flow rate: 1 mL/min
Injection: 4 μL
1.2 Amoxicillin Sodium
H O
NHH 2N H
N
S
O
H H
O
C H 3
C H 3
O
O N a
H
min0 2 4 6
mAU
-20
0
20
40
60
80 Amoxicillin Sodium
1.3 Cefradine
Column: Unisol C18 4.6×150mm 5μm
Part No.: UO951505-0
Mobile phase: Water/MeOH/3.86%
sodiumacetate solution/4
acetic acid solution 742:240:15:3
Detection: UV 254 nm
Flow rate: 1 mL/min
Injection: 2μL
1.4 Cefaclor
NH
N
S
OO
O HO
C H 3
H 2N H H H
min0 5 10 15
mAU
0
50
100
150
200
250
300
350Cefradine
Column: Unisol C18 4.6×150mm 5μm
Part No.: UO951505-0
Mobile phase: 0.05mol/L phosphate
buffer pH 3.4 :ACN 92:8
Detection: UV 254 nm
Flow rate: 1 mL/min
Injection: 5μL
,H 2ONH
N
S
OO
O HO
C l
H 2N H H H
min0 2 4 6 8 10 12
mAU
0
10
20
30
40
50
60
70Cefaclor
HP
LC
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059
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1.5 Cefadroxil
Column: Unisol C18 4.6×150mm 5μm
Part No.: UO951505-0
Mobile phase: Water/MeOH/3.86%
sodium acetate solution/4
acetic acid solution
742:240:15:3
Detection: UV 254 nm
Flow rate: 1 mL/min
Injection: 2 μL
min0 2 4 6
mAU
0
50
100
150
200
250
Column: Unisol C18 4.6×150mm 5μm
Part No.: UO951505-0
Mobile phase: 0.05mol/L phosphate
buffer pH 5.5 :ACN 96:4
Detection: UV 230 nm
Flow rate: 1 mL/min
Injection: 2μL
1.6 Cefalexin
1.7 Cefotaxime Sodium
Column: Unisol C18 4.6×150mm 5μm
Part No.: UO951505-0
Mobile phase: phosphate buffer
0.4mM KH2PO
4+8mM K
2HPO
4
/MeOH=89:11
Detection: UV 254 nm
Flow rate: 1 mL/min
Injection: 2 μL
1.8 Sulbactam Sodium + Cefoperazone Sodium
Column: Venusil XBP C18,
(4.6×250 mm 5 μm)
Part No.: VX952505-0
Mobile phase: 0.005 mol/L TABOH
(adjust pH to 5.0 with
H3PO
4):ACN 70:30
Detection: UV 220nm
Flow rate: 1 mL/min
Injection: 2 μL
NH
N
S
OO
OHO
CH3
H2N H H H
,H2O
min0 2 4 6 8 10
Norm.
0
20
40
60
80
100 Cefalexin
N
S
NH
H2N
N
O
OCH3
N
SH H
O
O ONa
O CH3
O
min0 2 4 6 8 10 12 14
Norm.
0
10
20
30
40
50
60
70
80 Cefotaxime Sodium
min0 2.5 5 7.5 10 12.5 15
mAU
0
100
200
300
400
500
Sulbactam Sodium
Cefoperazone Sodium
O
N
NaO
H
S
OO
CH3
CH3
H
O NH
N
S
OO
ONO
HN H H H
O
OH
N
N
O
O
CH3
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
OO
O OH
N
H2N H
NH
H HS
CH3
,H2O
HP
LC
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060
AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
1.9 Acetylspiramycin
Column: Venusil XBP-C8, (4.6×150 mm
5 μm)
Part No.: VX851505-0
Mobile phase:ammonium formate 0.2mol/L
adjust to pH 7.3 with TEA :
ACN=62:38
Detection: UV 232nm
Flow rate: 1 mL/min
Temperature: 30
Column: Unisol C18 4.6×250mm 5μm
Part No.: UO952505-0
Mobile phase: ACN:NaClO4/ H
3PO
4pH 2.2
=30:70
Temperature: 30
Flow rate: 0.8 mL/min
Injection: 20 μL
1.10 Meleumycin
Column: Venusil XBP C18, (4.6×250 mm
5 μm)
Part No.: VX952505-0
Mobile phase: H3PO
4 (0.025 mol/L
adjust to pH 3.0 with
TEA):ACN 87:13
Detection: UV 278nm
Flow rate: 1 mL/min
Injection: 20 μL
2.2 Ofloxacin
Column: Venusil XBP C18,
(4.6×250 mm 5 μm)
Part No.: VX952505-0
Mobile phase NH4AC/ KClO
4
(40mM , pH 2.0):ACN=85:15
Detection: UV 294nm
Flow rate: 1 mL/min
Injection: 4 μL
2.1 Norfloxacin
2.Synthetic antimicrobial agents
H
N
N N
F
C H 3
O H
OO
m in0 2 . 5 5 7 . 5 1 0 1 2 . 5
mAU
0
50
100
150
200
N o r f lo x a c in
N
N
H 3C
N
O
F
O
C O O H
H
C H 3
m in0 2 4 6 8 1 0
mAU
0
100
200
300
400
500
600
700
800
0
0 10 20 30 40 50 60 m
20
40
60
80
100
120
140
mAU
O
OH
HO
O
O
O
O
O
O
O OH
O O
HO
N
N
0
0 5 10 15 20 25 30 35 40 45 50 55 60 65 70-1
1
2
3
4
5
6
7
8
9
10
1.2
40
9.3
15
11
.21
5
18
.61
52
0.6
48
29
.98
2
52
24
8
57
.31
5
49
.04
8
HP
LC
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Best Value
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2.3 Ciprofloxacin Lactate
Column: Venusil XBP C18,
(4.6×150 mm 5 μm)
Part No.: VX951505-0
Mobile phase: citric acid buffer (0.05
mol/L, adjust to pH 3.5
with TFA):ACN=82:28
Detection: UV 277nm
Flow rate: 1 mL/min
Injection: 10 μL
H N
N N
O
F C O O H
min0 1 2 3 4 5 6 7
mAU
0
25
50
75
100
125
150
175
200
Ciprofloxacin Lactate
Column: Venusil XBP C18(L),
(4.6×150mm 5 μm)
Part No.: VX951505-L
Mobile phase:1%TEA pH 4.5 :ACN=87:13
Detection: UV 325nm
Flow rate: 1.5 mL/min
Injection: 10 μL
Temperature: 40
2.4 Gatifloxacin hydrochloride
2.5 Fluconazole
Column: Venusil XBP C18, (4.6×150 mm
5 μm)
Part No.: VX951505-0
Mobile phase: KH2PO
4 buffer(adjust to
pH7.0 with NaOH):MeOH 55:45
Detection: UV 260nm
Flow rate: 1 mL/min
Injection: 10 μL
Column: Venusil XBP-C8, (4.6×250 mm
5 μm)
Part No.: VX852505-0
Mobile phase: MeOH/ACN/KH2PO
4
0.075 mol/L /citric acid
1mol/L 30:30:36:4
Detection: UV 254nm
Flow rate: 1 mL/min
Injection: 2 μL
N
N
N C H 2 C
O H
C H 2 N
N
N
F
F
min0 1 2 3 4 5 6 7
mAU
0
50
100
150
200
250
300Fluconazole
3.1 Rifampicin
3.Anti-virus medicine
min0 5 10 15 20
mAU
0
50
100
150
200
250Rifampicin
O
N
N
N
H 3C
OC H 3
O H
O
H 3C O
C H 3
O
O
H 3C
H 3C
C H 3
H 3C
C H 3
O
O HN H
C H 3
C H 3
O H
C H 3
N=8763.37
As=1.39
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
6.9
88
06
04
02
00
0 5 10 15 20 25 30
06
04
02
00
O
O
OH
F
F
F
HP
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AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
3.2 Ganciclovir
Column: Unisol C18 4.6×150mm 5μm
Part No.: UO951505-0
Mobile phase:Water:MeOH 95:5
Detection: UV 252nm
Flow rate: 1 mL/min
Injection: 2 μL
4.2 Triamcinolone Acetonide
Column: Unisol C18 4.6×150mm 5μm
Part No.: UO951505-0
Mobile phase: MeOH:Water 21:19
Detection: UV 240nm
Flow rate: 1 mL/min
Injection: 2 μL
4.3 Hydrocortisone
Column: Unisol C18 4.6×150mm 5μm
Part No.: UO951505-0
Mobile phase: MeOH:Water 70:30
Detection: UV 240nm
Flow rate: 1 mL/min
Injection: 10 μL
Column: Unisol C18 4.6×150mm 5μm
Part No.: UO951505-0
Mobile phase:MeOH:Water 70:30
Detection: UV 281nm
Flow rate: 1 mL/min
Injection: 2 μL
4.1 Ethinylestradiol
4. Steriod Hormones
HN
N N
N
OOH
OH
H2N
O
min0 2 4 6 8 10 12 14
Norm.
0
20
40
60
80
100
120
Ganciclovir
HO
H H
C
OHCH3
H
CH
min0 1 2 3 4
Norm.
0
200
400
600
800
1000
Ethinylestradiol
O
CH3
CH3
F
O
O
CH3
OHH
HO O
CH3
HH
H
min0 2 4 6 8
Norm.
0
50
100
150
200
250
300
350
400Triamcinolone Acetonide
O
CH3 H
CH3HO
H
H H
OH
OOH
min0 1 2 3 4 5 6 7
Norm.
0
50
100
150
200
Hydrocortisone
HP
LC
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Innovation to Benefit Customers
4.4 DexamethasoneColumn: Venusil XBP C18,
(4.6×150 mm 5 μm)
Part No.: VX951505-0
Mobile phase: citric acid buffer (0.05
mol/L, adjust to pH 3.5
with TFA):ACN=82:28
Detection: UV 277nm
Flow rate: 1 mL/min
Injection: 10 μL
m in0 2 4 6 8
N orm .
0
2 5
5 0
7 5
1 0 0
1 2 5
1 5 0
1 7 5
2 0 0
O
HOCH3
OH
O
CH3
OH
F
H
CH3
HH
Dexamethasone
Column: Unisol C18 4.6×150mm 5μm
Part No.: UO951505-0
Mobile phase:Water :ACN 30:70
Detection: UV 240nm
Flow rate: 1 mL/min
Injection: 2 μL
4.5 Levonorgestrel
H H
C
OH
H
CH
CH3
O
min0 2 4 6 8
Norm.
0
50
100
150
200
250 Levonorgestrel
4.6 Prednisone
O
H H
OH
HCH3
O OH
CH3O
min0 2 4 6 8 10 12 14
Norm.
0
50
100
150
200
250
Prednisone
Column: Unisol C18 4.6×150mm 5μm
Part No.: UO951505-0
Mobile phase: Water :THF :MeOH 668:250:62
Detection: UV 254nm
Flow rate: 1 mL/min
Injection: 2 μL
4.7 Clobetasol Propionate
Column: Unisol C18 4.6×150mm 5μm
Part No.: UO951505-0
Mobile phase: phosphate buffer 0.05
mol/L ,pH 2.5
/ACN/MeOH 425:475:100
Detection: UV 240nm
Flow rate: 1mL/min
Injection: 2μL
O
O
CH3HO
CH3
FH
H
H
Cl
O
CH3
CH3
O
H
min0 2 4 6 8
Norm.
0
10
20
30
40
50
60
70 Clobetasol Propionate
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
HP
LC
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AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
4.8 Fluocinonide
Column: Venusil XBP C18
4.6×150mm 5μm
Part No.: VX951505-0
Mobile phase: MeOH :ACN :Water=60:10:30
Detection: UV 240nm
Flow rate: 1 mL/min
Injection: 10 μL
5.3 Omeprazole
Column: Venusil XBP-C8 4.6×250mm
5μm
Part No.: VX852505-0
Mobile phase: NaH2PO4 Buffer
(0.01 mol/L, adjust to pH7.6
with H3PO4): ACN 60:40
Detection: UV 302nm
Flow rate: 1 mL/min
Injection: 4 μL
Column: Venusil XBP C18 4.6×150mm
5μm
Part No.: VX951505-0
Mobile phase:MeOH :0.77%
Ammonium acetate aq. 285:115
Detection: UV 320nm
Flow rate: 1 mL/min
Injection: 10 μL
O
CH3
F
H
H
CH3
H
OH
OH
O
O CH3
O
5.1 Ranitidine Hydrochloride
5 Medicine for Gastric Ulcer
ON
S
HN
HN
CH3
NO2
H3C
CH3 HCl
min0 1 2 3 4 5
mAU
0
50
100
150
200
250 Ranitidine Hydrochloride
min0 2 4 6 8 10
mAU
0
25
50
75
100
125
150
175Omeprazole
5.2 Cimetidine
Column: Venusil XBP C18
4.6×150mm 5μm
Part No.: VX951505-0
Mobile phase: MeOH:Water:H3PO
4:TFA
200:800:0.3:0.2
Detection: UV 220nm
Flow rate: 1mL/min
Injection: 10 μLm in0 1 2 3 4 5 6 7
mAU
0
100
200
300
400
500 Cimetidine
HN
N
S
HN
N
CN
HN
CH3
mAU
Fluoxinonide400
350
300
250
200
150
100
50
00 1 2 3 4 5 6 min
O
HN
N
N
O
O
HP
LC
Colu
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065
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Column: Unisol C18 4.6×250mm 5μm
Part No.: UO952505-0
Mobile phase: water(0.3%TEA):ACN=75:25
Detection: UV 254nm
Flow rate: 1 mL/min
Temperature: 25
6.1 Quaternary Ammonium Alkaloids from Coptidis
6. Analysis of Alkaloids
AU
0.00
0.05
0.10
0.15
0.20
0.25
0.00 5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00 45.00 50.00 55.00 60.00
Column: Venusil XBP-NH2
4.6×250mm 5μm
Part No.: VN952505-0
Mobile phase:ACN:Ethanol:3% H3PO4 aq.
80:10:10
Detection: UV 220nm
Flow rate: 1 mL/min
Injection: 20 μL
6.2 Matrine
Mobile phase: ACN: SDS/H3PO
440:60
Detection: UV 210nm
Flow rate: 1mL/min
6.3 Ephedrine from Coptidis
min0 5 10 15 20 25 30
mAU
0
5
10
15
20
25
VWD1 A, Wavelength=210 nm (F:\GTQWJ\MH000045.D)
Ephedrina hydrochloridum
Venusil XBP C18 2
4.6×150 mm, 5μm
Part No.: VX951505-2
min0 5 10 15 20 25 30
mAU
-2.5
0
2.5
5
7.5
10
12.5
15
17.5
VWD1 A, Wavelength=210 nm (YXN\07091821.D)
Ephedrina hydrochloridum
Brand Z C18 (4.6×150 mm, 5μm)
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
VWD1 A Wavelength=220nm(NEWSHIYP004.D)
9.0
42
3.2
95
mAU
80
60
40
20
0
0 5 10 15 20 25 min
HP
LC
Colu
mns
066
AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
6.5 Aconitine Mesaconitine Hypaconitin
Mobile phase: MeOH: CH3COONa aq.
(0.03mol/L, adjust pH to
3.5with Acetic acid) =15:85
Detection: UV 238nm
Flow rate: 1 mL/min
Mobile phase: ACN: 2% Acetate Buffer
adjust pH to 6.5 with TEA
15:85
Detection: UV 235nm
Flow rate: 1 mL/min
6.6 Morphine from Papaver somniferum
Promosil C18 4.6×250mm 5μm
Part No.: PM952505-0
min0 10 20 30
mAU
-50
0
50
100
150
200
250
VWD1 A, Wavelength=235 nm (WUTOU\ZHENGJ26.D)
mesaconitineaconitine
hpyaconitine
Venusil XBP C18 2
4.6×250 mm, 5 μm
Part No.: VX95505-0
min0 2.5 5 7.5 10 12.5
mAU
0
20
40
60
80
100
VWD1 A, Wavelength=238 nm (YXN\07091805.D)
Codeine phosphate
Morphine
Mobile phase: ACN: 1%TFA 35:65
Detection: UV 346nm
Flow rate: 1mL/min
6.4 Berberine from Phellodendron
N
O
O
+
CH3O
CH3O
Cl- H2O2,
Venusil XBP C18 2 4.6×250 mm 5μm
Part No.: VX952505-2
min0 2.5 5 7.5 10 12.5 15 17.5
mAU
0
10
20
30
40
50
60
70
VWD1 A, Wavelength=346 nm (TF\XBJ00079.D)
Berberine hydrochloride
Brand Y ODS 4.6×250 mm 5 μm
N
O
O
+
CH3O
CH3O
Cl- H2O2,
min0 2.5 5 7.5 10 12.5 15 17.5
mAU
0
20
40
60
80
100
120
VWD1 A, Wavelength=346 nm (TF\XBJ00072.D)
Berberine hydrochloride
HP
LC
Colu
mns
067
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Column: Venusil XBP C18
4.6×150 mm 5 μm
Part No.: VX951505-0
Mobile phase: ACN:Water=70:30
Detection: UV 225nm
Flow rate: 1 mL/min
Injection: 5 μL
Temperature: 25
7.2 Haloxyfop-P-methyl
7.1 Analysis of 14 Kinds Herbicides
7 Agricultural Chemical
Sample: dazomet Cymoxanil Triadimefon Tebuconazole
Thiophanate-Methyl hexaconazole flutriafol
Iprodione Metalaxyl Procymidone carboxin
Prochlora Diethofencar Triadimenol
1,2,3,6-tetrahydro-N-(trichloromethylthio)phthalimide
Column: Venusil XBP C18 4.6×150mm 5μm
Part No.: VX951505-0
Mobile phase: A: Ammonium acetate buffer B:ACN
Gradient: 40%B to 45%B in 8 min, hold for 40min.
Detection: UV 225nm Flow rate: 1 mL/min
Injection: 20 μL Temperature: 25
AU
0.0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
2.0 4.0 6.0 8.0 10.0 12.0 14.0 16.0
A
0.00 5.00 10.00 15.00 20.00 25.00 30.00 35.00 40.00 45.00
0.0040
0.0035
0.0030
0.0025
0.0020
0.0015
0.0010
0.0005
0.0000
-0.0005
7.3 Fluazifop-p-butyl
Column: Venusil XBP C18
4.6×150 mm 5 μm
Part No.: VX951505-0
Mobile phase:ACN:Water=70:30
Detection: UV 225nm
Flow rate: 1 mL/min
Injection: 5 μL
Temperature: 25
AU
0.0
0.2
0
0.4
0
0.6
0
0.8
0
1.0
0
1.2
2.0 4.0 6.0 8.0 10.0 12.0 14.0 16.0 18.0 20.0
Column: Venusil XBP C18
4.6×150 mm 5 μm
Part No.: VX951505-0
Mobile phase:Water:MeOH =25:75
Detection: UV 254nm
Flow rate: 1 mL/min
Injection: 10 μL
Temperature: 25
7.4 1,2-Dibenzoyl-1-tert-butylhydrazine
AU
0.00
0.20
0.40
0.60
0.80
1.00 2.00 3.00 4.00 5.00 6.00 7.00 8.00 9.00 10.00
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
HP
LC
Colu
mns
068
AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
8.1 Standards of Amino Acids
8.2 Amino Acid from Salmon Calcitonin
8.Analysis of Amino Acid
Column: Venusil AA 4.6×250 mm 5 μm
Part No.: AA952505-0
Mobile phase: A: 0.1mol/L CH3COONa(Adjust pH 6.5 with Acetic Acid):Acetonitrile (93:7)
B:Water: Acetonitrile (4:1)
Gradient:
Flow rate: 1mL/min
Detection: UV 254nm
Temperature: 40
Sample: PTC-AA (derivatization of amino acids with PITC)
Time(min)
%B
0
0
11
7
13.9
12
14
15
29
34
29.1
100
37
100
37.1
0
45
0
HP
LC
Colu
mns
069
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8.3 Amino Acid from Terlipressin
Mass system: API Qtrap 3200 Applied Biosystem
Mass condition: Cation Detector Mode,MRM
HPLC Column: Venusil ASB C18 Column 2.1mm×50mm Part No.: VS950502-0
Sample: Prepared sample by SPE(Cleanert PCX 60mg/3ml;Part No.: CX0603)
Mobile Phase: A: 1% Formic Acid in Water B: Methanol
Gradient: 20%B to 95%B in 2 min, hold for 0.5 min, then switch to A:B(20:80)
Flow Rate: 0.5 mL/min
Temperature: 25
9.1 Pseudoephedrine in Plasma
9 Applications in LC-MS
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
CI
HC N N OOH
O
2HCL
Pseudoephedrine
Sample
Standards of Amino Acids
Pseudoephedrine
0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2Time.min
2.12
2.0e4
0.0
4.0e4
6.0e4
8.0e4
1.0e5
1.2e5
1.4e5
1.6e5
1.8e5
2.0e5
2.2e5
2.4e5
2.6e5
2.8e5
Lignocaine
0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2Time.min
1.47
0.5e5
1.0e5
1.5e5
2.0e5
2.5e5
3.0e5
3.5e5
4.0e5
HP
LC
Colu
mns
070
AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
9.2 Oleic Acid in Plasma
Mass system: API Qtrap 3200 Applied Biosystem
Mass condition: Anion Detector Mode,MRM
HPLC Column: Venusil ASB C18 Column 2.1mm×50mm Part No.: VS950502-0
Sample: Prepared sample by SPE(Cleanert PEP 60mg/3ml;Part No.: PE0603)
Mobile Phase: A: 13mmol/L ammonium acetate aq. ;B: Acetonitrile
Gradient: 5%B to 95%B in 2 min, hold for 2 min., switch to A:B(95:5) then hold for 2 min.
Flow Rate: 0.8 mL/min
Temperature: 25
Oleic acid
OH
O
1.44
3.43
00.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0
1000
2000
3000
0
4992
4000
Inte
nsity.
cp
s
Time.min
00.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0
2.0e5
4.0e5
6.0e5
8.0e5
1.0e6
1.2e6
1.4e6
Inte
nsity.
cp
s
Time.min
00.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0
1.0e5
2.0e5
3.0e5
4.0e5
5.0e5
Inte
nsity.
cp
s
Time.min
4.56
HP
LC
Colu
mns
071
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10.1 Glipizide
10.others
Column: Unisol C18 4.6×150mm 5μm
Part No.: UO951505-0
Mobile phase: phosphate Buffer
0.1 mol/L ,pH 6.0 :MeOH=55:45
Flow rate: 1mL/min
Injection: 20 μL
10.2 Terfenadine
Column: Unisol C18 4.6×150mm 5μm
Part No.: UO951505-0
Mobile phase:MeOH: H3PO
4/Triethylamine
(0.1mol/L) 80:20
Detection: UV 254nm
Flow rate: 1 mL/min
Injection: 10 μL
10.3 Dopamine and Its Metabolin
Sample: Dopamine Isoprenaline Soprenaline
Column: Venusil F5PH 4.6×250mm 5μm
Part No.: VF952505-0
Mobile phase: MeOH: CH3COOH /CH3COONH4 Buffer (pH 4.5) =15:85
Detection: UV 280nm
Flow rate: 0.8 mL/min
Dopamine Isoprenaline
05101409.D: UV Chromatogram, 280 nm
0
20
40
60
Intens.
mAU
0 5 10 15 20 25 30 35 40 Time [min]
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
N =7587
AS=1.03
N=8 249
As=1.00
HP
LC
Colu
mns
072
AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
10.6 Saccharin Sodium in Milk Powder
Sample: Benzoic acid, 2,4-
Hexadienoic acid, Sodium
saccharine, milk powder
Column: Venusil Unisol C18
4.6×250 mm 5 μm
Part No: UO952505-0
Mobile phase:MeOH:CH3COONH
4
(0.02 M) =5:95
Detection: UV 230nm
Flow rate: 1 mL/min
Temperature: 30
Sample: Formic acid, acetic acid, maleic
acid, succinic acid, fumaric acid
Column: Unisol C18 4.6×250 mm 5 μm
Part No.: UO951505-0
Mobile phase: 0.02M NH4AC
Aq.:MeOH=95:5
Detection: UV 215nm
Flow rate: 1 mL/min
Injection: 10 μL
Temperature: 20
10.5 Catechins in Tea
Sample: Extract of tea
Column: Venusil Unisol C18
4.6×250 mm 5 μm
Part No.: UO952505-0
Mobile phase: 0.02%H3PO
4: MeOH=81:19
Detection: UV 278nm
Flow rate: 1 mL/min
Temperature: 40
10.4 Organic Acids
Determination of organic acids in Fermentation Broth
M inutes
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 22.5 25.0 27.5 30.0
Vo
lts
0 .00
0.05
0.10
0.15
Pe
rce
nt
80
85
90
95
100
1
2
3
4
Standard
M inutes
0 5 10 15 20 25 30 35 40 45 50
Vo
lts
0 .0
0.1
0.2
0.3
0.4
0.5
Pe
rce
nt
80
85
90
95
100
1
2
HP
LC
Colu
mns
073
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Determination of organic acids in Fermentation Broth
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
10.8 Tartrazine in Food
Column: Unisol C18
4.6×250 mm 5 μm
Part No.: UO951505-0
Mobile phase: A: Ammonium acetate
buffer pH 4.0 ;B: MeOH
Gradient: 35%B to 50%B in 5 min.
Detection: UV 254nm
Flow rate: 1 mL/min
Injection: 10 µL
10.9 Melamine in Feed
Column: Venusil ASB C18
4.6×250 mm 5 μm
Part No.: VS952505-0
Mobile phase:10mM Citric acid+
10mM Perfluorooctane
sulfonate( pH 3.0) :ACN=85:15
Detection: UV 240nm
Flow rate: 1 mL/min
Injection: 10 µL
Temperature: 40
10.7 DHA in the Health Food
Column: Unisol C18
4.6×250 mm 5 μm
Part No.: UO951505-0
Mobile phase:MeOH:Water =80:20
Detection: UV 254nm
Flow rate: 1 mL/min
Injection: 10 µL
HP
LC
Colu
mns
074
AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
10.10 Tetrodotoxin TTX
Column: Venusil ASB C18
4.6×250 mm 5 μm
Part No.: VS952505-0
Mobile phase: 0.02% H3PO
4:MeOH=40:60
Detection: UV 200nm
Flow rate: 0.5mL/min
Temperature: 25
10.11 Baicalin
Column: Venusil ASB C18,
(4.6×150 mm 5 μm)
Part No.: VA951505-0
Mobile phase: MeOH : 1% acetic
acid =50:50
Detection: UV 274nm
Flow rate: 1 mL/min
Injection: 5 μL
0
4
2
6
8
10
12
1 2 3 4 5 6 7 8 9 ml
OHO
HO
NH OH
OH
OH
OO
HN
H2N=
SS
S
racemate
mA
OH
OH
OH
OH
HO
HO
O
OO
O
O
0.0 2.5 5.0 7.5 10 12.5 15 17.5 20 22.5 25 27.5 30
0.00
0.05
0.10
0.15
25
50
75
100
HP
LC
Colu
mns
075
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Column Cleaning Procedures
Due to interactions between the stationary phase and
sample components, HPLC columns may occasionally
require cleaning or regeneration. The following conditions
apply to silica-based columns. Flow rates should be
1/5 - 1/2 of the typical flow rate.
To estimate the column volume, use the following
equation:
V = column volume in mL
r = column radius in cm
L = column length in cm
APPENDIX
V=pr2 / L
UNBONDED SILICA COLUMNS (SILICA) Rinse with 10 column volumes each of: Hexane, Methylene, Chloride,
Isopropanol, Methylene Chloride. Mobile phase: Flush column with 30mL
2.5% 2,2-dimethoxy- propane and 2.5% glacial acetic acid in hexane
Rinse with 10 column volumes of: 95% Water/5% Acetonitrile (for buffer
removal) followed by 95% Acetonitrile/5% Water mobile phase
Rinse with 20 column volumes of mobile phase with buffer removed run
gradient (2x):
A) 0.1% Aqueous TFA in Water
B) 0.1% TFA in Acetonitrile/Isopropanol (1:2) 25% B 100% B for 30 minutes
Equilibrate with 10 column volumes of mobile phase.
Rinse with 10 column vol umes each of : Chloroform,Isopropanol, Methylene
chloride, mobile phase. Exception: Recommended for cleaning Luna Amino
when used in reversed phase mode:
1. Wash with at least 30 column volumes of Sodium Hydroxide (pH 11.0)
2. Flush with at least 30 column volumes of Water (HPLC grade)
3. Re-equilibrate to Mobile phase conditions.
(300 X 7.8mm size columns) Rinse with 5 column volumes of:
0.1M Phosphate buffer pH 3.0. For strongly retained proteins: Run 100%
Water to 100% Acetonitrile to 100% Water over 60 minutes or wash with
5 column volumes of SDS or 6M Guanidine Thiocyanate or 10% DMSO
Rinse with 10 column volumes of:
500mM Phosphate buffer pH 7 10% Acetic acid (Aq)
5 Column Volumes of Water
10 Column Volumes of Phosphate buffer pH 7
5 Column Volumes of Water
10 Column Volumes of Methanol
10 Column Volumes of Water
For protein removal
Follow the above procedure with this exception: Substitute 10 column
volumes of Methanol with 10 column volumes of 5M Urea or 5M
Guanidine Thiocyanate
REVERSED PHASE COLUMNS
(C18, C8, C4, C2, C1, PHENYL, CN, NH2)
REVERSED PHASE PROTEIN/PEPTIDE COLUMNS
(C18, C8, C5, C4, PHENYL)
BONDED NORMAL PHASE COLUMNS
(CN, NH2, DIOL )
GFC/SEC COLUMNS FOR PROTEINS
ION-EXCHANGE COLUMNS
(SAX, SCX, NH2, DEAE)
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
HP
LC
Colu
mns
076
AgelaTechnologiesBETTER SOLUTION FOR CHROMATOGRAPHY
The Methods of Maintaining Good Column Lifetime and PerformanceInject only well-prepared (filtration, liquid/liquid extraction, SPE) clean samples
Minimize pressure surge; avoid mechanical and thermo shock.
Use guard columns or on-line filtration.
Flush columns frequently using an appropriate program
Remove unstable and strongly retained components of no-interest from samples
Use low pH (1-6) mobile phase if possible.
Use organic buffer when operating at medium to high pH (6-10)
Avoid elevated temperature unless it is necessary
Add 200 ppm sodium azide in aqueous mobile phase to suppress the growth of the bacteria
Wash out all buffer salts and store the columns in high organic solution for overnight or long time storage
SPEColumns
and Wellplates
Cleanert SPE Columns, Wellplates and Media
Cleanert Plus SPE Columns and 96 Wellplates-NEW
OMM Technology for SPE-NEW
SLE Technology and Sorbents
Bonded Silica SPE
Non-silica Adsorption Sorbent
Mixed and Layered Phases
Specialized Phases
Cleanert Pre-IC
The Development of SPE Method
Applications
Cross-reference Table
078
080
082
089
091
097
099
100
102
103
106
114
GC
Colu
mns
Cleanert SPE Columns, Wellplates and Media
Agela Technologies provides a full line of SPE sorbents, columns and wellplates.
OMM SPE Products (Optimized Molecular Modification)- PEP Plus, PEP, PAX, PCX, PWAX, PWCX and PS are all based on
polystyrene/divinylbenzene while each phase has different functionality and unique selectivity. They are highly recommended for the extrac-
tion of a wide range of compounds in pharmaceutical, agricultural, food, and environmental industries. PEP and PEP Plus are good replace-
ment of Water Oasis® HLB. Average particle diameter: 35-70μm; Average pore size: 80Å; Specific surface area: 600m2/g.
SLE(Solid supported Liquid/liquid Extraction) columns and well-plates
Specially treated diatomite materials are packed in columns and well plates. The liquid/liquid extraction is run on the surface of the materials,
which is easily automated in parallel and time-saving.
Bonded silica based SPE column
C18, C18-N, C8, NH2, COOH, Silica, PSA, PRS, SCX, SAX; All of them are made of high quality and low metal silica particles. Using the
special surface modification methodology, the activity of silica surface is reduced largely, which in turn will reduce the tailing of compounds
and will ensure high recovery and good reproducibility.
Characteristics of silica particles:
Average particle diameter: 50μm
Average pore size: 60Å
Volume of pore: 0.8cm2/g
Specific surface area: 480m2/g
Surface of silica: made by special surface modification
AgelaTechnologies
BETTER SOLUTION FOR CHROMATOGRAPHY
Cle
anert
SP
E
078
Best Value
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Innovation to Benefit Customers
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
Cle
anert
SP
E
079
Non-silica adsorption phase columns-Florisil, PestiCarb, Alumina(N, A, B). They are commonly used to remove polar interference from
non-polar samples. Cleanert adsorption sorbents have high purity, high recovery and good reproducibility. They are widely used in sample
preparation for environmental and food analysis.
Mixed and layered phases-C8/SCX, PestiCarb/NH2, PestiCarb/PSA
Specialized phases-SUL-5, DNPH-Silica,HXN
PEP,HXN,
PS,PAX,PCX,
PWAX,PWCX
PEP,Plus
Polymer SPE
Sorbents
Florisil,PestiCarb,
Alumina-N,
Alumina-A,
Alumina-B
Non-silica
Adsorption
Sorbents
DNPH-Silica
SUL-5,
HXN
Special SPE
Columns
C8/SCX
PestiCarb/NH2
Mix Sorbents
SLE
SLE Plus
Solid Supported
Liquid/liquid
Extraction
Cleanert SPE
Products
ODS,C8,CN,NH2 ,
COOH,Silica,PSA,
PRS,SCX,SAX,
Diol
Sorbents
Based on
Bonded Silica
GC
Colu
mns
AgelaTechnologies
BETTER SOLUTION FOR CHROMATOGRAPHY
Cle
anert
SP
E
080
Cleanert Plus SPE products use the particles with narrowly controlled size distribution, a novel frit material & design, unique pre-activation
process on reverse phase, and a pre-drying on normal phase columns.
Stable flow rate, clogging free:
The usual problem of cartridge clogging caused by the sticky and slimy samples can be completely eliminated by using the PEP Plus prod-
uct line, which makes the sample preparation easier, faster, and more reliable than ever.
No activation step is needed, saving 1/3 of work load and time:
Using the new Cleanert PEP Plus can save you 1/3 of your work load eliminating the pre-activation steps. PEP Plus does not suffer from
vacuum-drying effect, and has balanced adsorption for a wide range of analytes. PEP Plus is the first choice for reverse phase SPE meth-
ods development.
More consistent performance from normal phase by pre-drying:
The new Cleanert Florisil Plus, Cleanert Alumina Plus, Cleanert Pesticarb Plus , Cleanert Silica Plus and Cleanert NH2 Plus will give an
unprecedented performance consistency owing to the use of special frits, narrow particle distribution, and pre-drying features.
Faster and reliable alternative to liquid/liquid extraction:
The new Cleanert SLE Plus Solid Supported Liquid/Liquid Extraction columns and plates use specially treated diatomite materials to run
liquid/liquid extraction on solid supports. Cleanert SLE Plus products are designed for automation and parallel process for time saving.
Description
Easier, faster and more reliable!
Benefits:
SPE-HPLC Analysis of Terramycin in Serum of Mouse using Cleanert PEP Plus
Application Examples:
Cleanert Plus SPE Columns and 96 Wellplates
a. Wash with 2mL methanol, 2mL water Preconditioned
12 mins by gravity 0 mins
b. Load 2mL sample solution(the mouse serum which Load 2mL sample solution(the mouse serum which contains 10ppm
contains 10ppm terramycin); 15 mins by gravity terramycin); 4 mins by gravity
c. Wash with 3mL water, then drying the column fully Wash with 3mL water, then drying the column fully
d. Elute terramycin with 3mL methanol Elute terramycin with 3mL methanol
e. Evaporate the elution by nitrogen at room temperature; Evaporate the elution by nitrogen at room temperature; dissolve
dissolve the residue with 2mL mobile phase the residue with 2mL mobile phase
f. Recovery: 83.2% Recovery: 81.4%
method and the result of Cleanert PEP 60mg/3mL method and the result of Cleanert PEP Plus 60 mg/3mL
Material Sorbent Vol Tubes/box Cat. Number
Ordering Information
30mg
50mg
60mg
100mg
200mg
500mg
15mg/well
30mg/well
50mg/well
A hydrophilic and
lipophilic balanced
material, made of
polydivinyl benzene
functionalized with
a polar group
100
100
50
50
30
30
96-wellplate
96-wellplate
96-wellplate
PE0301-P
PE0501-P
PE0603-P
PE1003-P
PE2006-P
PE5006-P
PE0152-WP
PE0302-WP
PE0502-WP
1mL
1mL
3mL
3mL
6mL
6mL
2mL
2mL
2mL
Cleanert PEP Plus
New Product!
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Cle
aner
t SP
E
081
The new Cleanert Florisol Plus SPE products features a special frit design which made these columns clogging-free. The SPE columns also
offer an unique “pre-drying” function to samples by removing water that is contained in the samples. These columns have unprecedented
consist performance.
Average particle diameter: 80-100μm
Pore size: 80Å
Specific surface area: 250-300m2/g
Description
Cleanert Florisil Plus
Material Sorbent Vol Tubes/box Cat. Number
Ordering Information
100mg
200mg
500mg
500mg
1g
2g
100mg/well
Sodium
sulfate/Florisil
100
100
50
50
30
15
96-wellplate
FS1001-P
FS2003-P
FS5003-P
FS5006-P
FS0003-P
FS2000-P
FS1002-WP
1mL
3mL
3mL
6mL
6mL
10mL
2mL
The new Cleanert Silical Plus SPE products features a special frit design which made these columns clogging-free. The SPE columns also
offer an unique “pre-drying” function to samples by removing water that is contained in the samples. These columns have unprecedented
consist performance.
Average particle diameter: 40-60μm
Pore size: 60Å
Specific surface area: 480m2/g
Description
Cleanert Silica Plus
Material Sorbent Vol Tubes/box Cat. Number
Ordering Information
100mg
200mg
500mg
500mg
1g
50mg/well
100mg/well
Sodium
sulfate/Silica
100
100
50
50
30
96-wellplate
96-wellplate
SI1001-P
SI2003-P
SI5003-P
SI5006-P
SI0006-P
SI0502-WP
SI1002-WP
1mL
3mL
3mL
6mL
6mL
2mL
2mL
GC
Colu
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AgelaTechnologies
BETTER SOLUTION FOR CHROMATOGRAPHY
Cle
anert
SP
E
082
Re
tain
ed
on
th
e s
orb
en
t
Re
tain
ed
on
th
e s
orb
en
t
OMM Technology (Optimized Molecular Modification)
for SPE
Agela’ s SPE products have been developed based on a thorough understanding of the science, e.g., interactive natures of chemical mol-
ecules. Our SPE products can thus better meet customer’ s needs. Our R&D results demonstrated that the adsorption/desorption property
of the polymeric SPE materials is regulated by the types of the functional groups and the degree of substitution of the surface modification.
In general, modification with electron donor groups will help to retain the electron-deficient molecules, while modification with electron-
withdrawing groups will prefer to retain the electron-rich molecules. Different SPE materials have been developed by incorporating proper
types of functional groups and degree of substitutions on the surface, and thus providing optimized and balanced performance for all types of
molecules.
Cleanert PEP (Polar Enhanced Polymer) is a polymer based SPE sorbent which consists of hydrophilically modified divinyl benzene. Clean-
ert PEP polymer sorbent is water-wettable and it does not suffer from column drying effect. It thus offers consistent performance due to
proper wetting capability which is especially important for 96-well plates. It also has high retention for polar compounds and high capacity for
a wide spectrum of analytes.
The Cleanet PEP (polar polymer) sorbent is a unique hydrophilic-lipophilic balance material. It provides excellent wettability since the
sorbent hydrophobic surface is highly modified with polar functional groups. The SPE mechanism follows the reverse phase separation prin-
ciple.
Good Water-Wettability
Effect of Drying on Recovery - PEP Versus C18 Sorbents
Re
tain
ed
on
th
e s
orb
en
t
The electron donating and withdrawing functionalities of the sorbent allows it to have enhanced retention for polar compounds.
High Retention for Polar Compounds
New Product!
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Cle
anert
SP
E
083
Re
tain
ed
on
th
e s
orb
en
t
PEP sorbents are water-wettable, maintaining high retention and high capacity for a wide spectrum of analytes, even when the SPE column
runs dry.
Consistent Performance for Acidic, Neutral and Basic Compounds
The PEP sorbent has 2-3x more surface area and shows a dramatic increase of sample capacity compared to silica-based C18. The volume
of elution solvents can be reduced, and subsequently the total operation time (including solvent evaporation) will be shortened
High Capacity
Re
tain
ed
on
th
e s
orb
en
t
The OMM technology offers consistent surface modifications and thus batch-to-batch reproducibility. Multiple batches of PEP, PCX, and PAX
have been successfully used for various compounds with consistent results.
Excellent Batch-to-Batch Reproducibility
GC
Col
umns
AgelaTechnologies
BETTER SOLUTION FOR CHROMATOGRAPHY
Cle
aner
t SP
E
084 Description PEP (polar polymers, alternative to Oasis HLB)
PEP is made of polydivinylbenzene on which the surface is functionalized with vinyl prolidone. The material has a balanced hydrophilic and
hydrophobic property and can be used in the entire pH range of 1-14.
PEP can be used to extract a variety of polar and non-polar compounds. Some highly hydrophilic compounds which have little retention on
C18 columns, such as chlorinated phenols, phosphate esters and drug metabolites, can be effectively retained on PEP
Particle Characteristics
Functionalized polymer sorbents; Average particle size: 35μm.
Average pore size: 80Å; Surface area: 600m2/g
PEP(Polar Polymers)
Material Sorbent Vol Tubes/box Cat. Number
30mg
60mg
100mg
200mg
500mg
30mg/well
50mg/well
10g
100g
Cleanert
PEP
100
50
50
30
30
96-wellplate
96-wellplate
PE0301
PE0603
PE1003
PE2006
PE5006
PE0302-W
PE0502-W
PE0010
PE0100
1mL
3mL
3mL
6mL
6mL
2mL
2mL
Ordering Information
Description
The PEP Plus columns are packed with high quality PEP sorbents. The sorbents contain the high surface area polydivinylbenzene spherical
particles that are modified with polar groups to offer a balanced hydrophilicity and hydrophobicity. The characteristics of the sorbent are:
water-wettable (don’ t suffer from undesirable drying effect), wide usable pH range (1-14), high retention for polar compounds, and high
capacity.
PEP Plus has a novel frit material & design, and it has undergone a pre-activation process step during manufacturing. This eliminates the
need for an activation step prior to use thus reducing working load by ~30%.more than 1/3 work load can be saved.
Particle Characteristics
Polar and spherica l particles: Average particle size: 40μm; Average pore size: 70Å ;Special surface area: 600m2/g
Material Sorbent Vol Tubes/box Cat. Number
Ordering Information
30mg
60mg
100mg
200mg
500mg
30mg/well
50mg/well
10g
100g
Cleanert
PEP Plus
100
50
50
30
30
96-wellplate
96-wellplate
PE0301-P
PE0603-P
PE1003-P
PE2006-P
PE5006-P
PE0302-WP
PE0502-WP
PE0010-P
PE0100-P
1mL
3mL
3mL
6mL
6mL
2mL
2mL
PEP Plus (Pre-conditioned and Clog Resistant) New Product!
Cle
anert
SP
E
085
Description
It is designed to overcome the limitations of traditional silica based mixed-mode SPE sorbents such as C18/SAX. It is a RP/strong anion-
exchange mixed mode polystyrene/divinylbenzene sorbent, stable from pH 0-14.
Particle Characteristics
Based on functioalized polystyrene/divinylbenzene;
Average particle diameter: 40μm; Average pore size: 70Å; Volume of pore: 1.2cm2/g; Specific surface area: 600m2/g.
PAX(RP/Strong Anion Exchange)
Material Sorbent Vol Tubes/box Cat. Number
Ordering Information
30mg
60mg
100mg
200mg
500mg
30mg/well
50mg/well
10g
100g
Cleanert
PAX
100
50
50
30
30
96-wellplate
96-wellplate
AX0301
AX0603
AX1003
AX2006
AX5006
AX0302-W
AX0502-W
AX0010
AX0100
1mL
3mL
3mL
6mL
6mL
2mL
2mL
Description
PCX is a mixed-mode, strong cation exchange sorbent. It has reverse-phase and anion-exchange dual functionality. It high surface area
and a wide usable pH range of 0-14.
Particle Characteristics
Based on Functionalized polystyrene/divinylbenzene;
Average particle diameter: 40μm; Average pore size: 70Å; Volume of pore: 1.2cm2/g; Specific surface area: 600m2/g.
PCX(RP/Strong Cation Exchange)
Material Sorbent Vol Tubes/box Cat. Number
Cleanert
PCX
Ordering Information
30mg
60mg
100mg
200mg
500mg
30mg/well
50mg/well
10g
100g
100
50
50
30
30
96-wellplate
96-wellplate
CX0301
CX0603
CX1003
CX2006
CX5006
CX0302-W
CX0502-W
CX0010
CX0100
1mL
3mL
3mL
6mL
6mL
2mL
2mL
Best ValueGuaranteed Product Quality
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BETTER SOLUTION FOR CHROMATOGRAPHY
Cle
anert
SP
E
086
Description
Cleanert PWCX provides the dual modes of retention, weak cation exchange and reverse phase on a stable polymer sorbent, which
improves the retention for basic analytes.
Particle Characteristics
Based on partially functionalized polystyrene/divinylbenzene;
Average particle diameter: 40μm; Average pore size: 70Å; Volume of pore: 1.2cm2/g; Specific surface area: 600m2/g.
Material Sorbent Vol Tubes/box Cat. Number
30mg
60mg
100mg
200mg
500mg
30mg/well
50mg/well
10g
100g
Cleanert
PWCX
100
50
50
30
30
96-wellplate
96-wellplate
WCX0301
WCX0603
WCX1003
WCX2006
WCX5006
WCX0302-W
WCX0502-W
WCX0010
WCX0100
1mL
3mL
3mL
6mL
6mL
2mL
2mL
Ordering Information
PWCX(RP/Weak Cation Exchange)
Description
Cleanert PWAX provides the dual modes of retention, weak anion exchange and reverse phase on a stable polymer sorbent , which
improves the retention for acidic analytes.
Particle Characteristics
Based on partially functionalized aminopolystyrene/divinylbenzene;
Average particle diameter: 40μm; Average pore size: 70Å; Volume of pore: 1.2cm2/g; Specific surface area: 600m2/g.
PWAX(RP/Weak Anion Exchange)
Material Sorbent Vol Tubes/box Cat. Number
Ordering Information
30mg
60mg
100mg
200mg
500mg
30mg/well
50mg/well
10g
100g
Cleanert
PWAX
100
50
50
30
30
96-wellplate
96-wellplate
1mL
3mL
3mL
6mL
6mL
2mL
2mL
WAX0301
WAX0603
WAX1003
WAX2006
WAX5006
WAX0302-W
WAX0502-W
WAX0010
WAX0100
Cle
anert
SP
E
087
Description
PEP-ED is made of the polydivinylbenzene having a surface functionalized with a electron donating group. They can be used in the entire pH
1-14. The surface has a balanced hydrophilic and hydrophobic property and can extract a variety of polar and non-polar compounds, espe-
cially having electron-deficient structures .
Particle Characteristics
Adsorption sorbents; Average particle size: 35μm.Average pore size: 80Å; Surface area: 600m2/g
Material Sorbent Vol Tubes/box Cat. Number
Cleanert
PEP ED
Ordering Information
PEP-ED
30mg
60mg
100mg
200mg
500mg
30mg/well
50mg/well
10g
100g
100
50
50
30
30
96-wellplate
96-wellplate
PE0301-E
PE0603-E
PE1003-E
PE2006-E
PE5006-E
PE0302-WE
PE0502-WE
PE0010-E
PE0100-E
Description
PEP-ER is made of the polydivinylbenzene having a surface functionalized with a electron withdrawing group . They can be used in the
entire pH 1-14. The surface has a balanced hydrophilic and hydrophobic property and can extract a variety of polar and non-polar com-
pounds, especially having a electron-rich structure
Particle Characteristics
Adsorption sorbents; Average particle size: 35μm.Average pore size: 80Å; Surface area: 600m2/g
PEP-ER
Material Sorbent Vol Tubes/box Cat. Number
Ordering Information
30mg
60mg
100mg
200mg
500mg
30mg/well
50mg/well
10g
100g
Cleanert
PEP ER
100
50
50
30
30
96-wellplate
96-wellplate
1mL
3mL
3mL
6mL
6mL
2mL
2mL
PE0301-R
PE0603-R
PE1003-R
PE2006-R
PE5006-R
PE0302-WR
PE0502-WR
PE0010-R
PE0100-R
1mL
3mL
3mL
6mL
6mL
2mL
2mL
Best Value
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umns
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BETTER SOLUTION FOR CHROMATOGRAPHY
Cle
aner
t SP
E
088
Description
PS is made of non-substituted polydivinylbenzene. It has larger surface areas (>600m2/g.) and thus greater capacity than reverse phase
bonded silica.PS can be used for the extraction of non-polar and polar compounds.
Particle Characteristics
Based on polystyrene/divinylbenzene; Average particle diameter: 40μm;
Average pore size: 70Å; Volume of pore: 1.2cm2/g; Specific surface area: 600m2/g.
Material Sorbent Vol Tubes/box Cat. Number
30mg
60mg
100mg
200mg
500mg
30mg/well
50mg/well
10g
100g
Cleanert
PS
100
50
50
30
30
96-wellplate
96-wellplate
PS0301
PS0603
PS1003
PS2006
PS5006
PS0302-W
PS0502-W
PS0010
PS0100
1mL
3mL
3mL
6mL
6mL
2mL
2mL
Ordering Information
PS
Cle
anert
SP
E
089
This technique utilizes a layer of solid material, which is a type of modified diatomaceous earth, as a support for liquid/liquid extraction.
SLE (Solid Supported Liquid/liquid Extraction)
Technology and Sorbents
Mechanism
A modified form of diatomaceous earth is packed in a cartridge.
Biological fluid can be applied to the cartridge using gravity flow.
The aqueous sample is deposited as a thin film on the hydrophilic surface.
The analytes are extracted with a water immiscible organic solvent.
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BETTER SOLUTION FOR CHROMATOGRAPHY
Cle
anert
SP
E
090
Material Sorbent Vol Tubes/box Cat. Number
200mg
500mg
500mg
1g
14g
300mg/well
500mg/well
Special
treated diatomite
100
50
50
30
10
96-wellplate
96-wellplate
SL2003
SL5003
SL5006
SL0006
SL1400
SL3002-W
SL5002-W
3mL
3mL
6mL
6mL
40mL
2mL
2mL
Ordering Information
The General Method Includes:
1. Load an aqueous sample into the column by gravity or a soft vacuum
2. Apply one or a multiple organic solvents by gravity or well-controlled vacuum
3. Vacuum the organic solution which contains the analyte out from bottom of the columns or plates, collect the solution
4. Concentrate the collected solution
Description
Solid Supported Liquid/Liquid Extraction columns and plates. They use the specially treated diatomite materials to run liquid/liquid extractions
on the solid supports, which are easily automated in parallel and time saving.
Compared with SLE, the SLE Plus has a special frit design which keeps the columns clogging-free, even for very nasty samples.
Material Sorbent Vol Tubes/box Cat. Number
200mg
500mg
500mg
1g
14g
300mg/well
500mg/well
Special
treated diatomite
100
50
50
30
10
96-wellplate
96-wellplate
SL2003-P
SL5003-P
SL5006-P
SL0006-P
SL1400-P
SL3002-WP
SL5002-WP
3mL
3mL
3mL
6mL
40mL
2mL
2mL
Ordering Information
The general method includes:
1. Load an aqueous sample into the column by gravity or a soft vacuum
2. Apply one or a multiple organic solvents by gravity or well-controlled vacuum
3. Vacuum the organic solution which contains the analyte out from bottom of the columns or plates, collect the solution
4. Concentrate the collected solution
SLE
SLE Plus
Description
Solid supported liquid/liquid Extraction columns and plates use specially treated diatomiteous materials as a solid support for liquid/liquid
extractions. The SPE procedures can be easily automated.
Cle
anert
SP
E
091
Description
ODS C18 products columns and plates are packed with reverse phase, octadecylsilane
bonded silica sorbents. The sorbent is double endcapped and has a high bonding density
(%C>17). These columns can be used as a replacement for BondElute C18 and Super clean
ENVI C18. These products can be used for desalting biomolecules, such as proteins and
DNAs.
Particle Characteristics
Based on silica; C%: 18-19%; Average particle diameter: 50μm; Average pore size: 60Å;
Volume of pore: 0.8cm2/g;Specific surface area: 480m2/g
Bonded Silica SPE
Material Sorbent Vol Tubes/box Cat. Number
Cleanert ODS
(C18,end-capped)
Ordering Information
100mg
200mg
500mg
500mg
1g
50mg/well
100mg/well
10g
100g
100
50
50
30
30
96-wellplate
96-wellplate
181001
182003
185003
185006
180006
180502-W
181002-W
180010
180100
1mL
3mL
3mL
6mL
6mL
2mL
2mL
Si
O
O
Si O
Si
Si (CH3)
3
(CH2)
17CH
3
Description
ODS C18-N products have silica based reverse phase sorbents bonded with octadecylsilane
without endcapping. The extra silanol residue of the sorbent provides additional polar inter-
actions associated with surface silanol groups which enhance the retention of basic com-
pounds. These columns are similar to Agilent AccuBond C18 and BondElute C18 OH.
ODS C18(End-capped)
ODS C18-N(Non-end-capped)
Particle Characteristics
Based on silica; C%: 17-18%; Average particle diameter: 50μm; Average pore size: 60Å;
Volume of pore: 0.8cm2/g;Specific surface area: 480m2/g
Material Sorbent Vol Tubes/box Cat. Number
Cleanert ODS-N
(C18,Non-end-capped)
Ordering Information
100mg
200mg
500mg
500mg
1g
50mg/well
100mg/well
10g
100g
100
50
50
30
30
96-wellplate
96-wellplate
1mL
3mL
3mL
6mL
6mL
2mL
2mL
181001-N
182003-N
185003-N
185006-N
180006-N
180502-N-W
181002-N-W
180010-N
180100-N
Si
O
O
Si OH
Si (CH2)
17CH
3
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BETTER SOLUTION FOR CHROMATOGRAPHY
Cle
anert
SP
E
092
The property of C8 products is similar to ODS C18 products. However, this sorbent is slightly
less retentive than C18, which facilitates the elution of more hydrophobic substance. C8 is
successfully used for the extraction of both water-soluble and fat-soluble vitamins from
serum, as well as the desalting of biological macromolecules.
Description
Particle Characteristics
Based on silica; C%: 9-10%; Average particle diameter: 50μm; Average pore size: 60Å;
Volume of pore: 0.8cm2/g;Specific surface area: 480m2/g
Material Sorbent Vol Tubes/box Cat. Number
Cleanert C8
Ordering Information
100mg
200mg
500mg
500mg
1g
50mg/well
100mg/well
10g
100g
100
50
50
30
30
96-wellplate
96-wellplate
1mL
3mL
3mL
6mL
6mL
2mL
2mL
081001
082003
085003
085006
080006
080502-W
081002-W
080010
080100
Cyano(CN) SPE have silica based sorbents bonded with cyanopropyl functional group. This
polar sorbent exhibits both polar and non-polar interactions. It can be used for extraction of
both polar and non-polar molecules in either normal phase reverse phase mode.
Description
C8 (Octyl)
CN (Cyanopropyl)
Particle Characteristics
Based on silica; C%: 5-6%; Average particle diameter: 50μm; Average pore size: 60Å;
Volume of pore: 0.8cm2/g;Specific surface area: 480m2/g
Material Sorbent Vol Tubes/box Cat. Number
Cleanert CN
Ordering Information
100mg
200mg
500mg
500mg
1g
50mg/well
100mg/well
10g
100g
100
50
50
30
30
96-wellplate
96-wellplate
1mL
3mL
3mL
6mL
6mL
2mL
2mL
CN1001
CN2003
CN5003
CN5006
CN0006
CN0502-W
CN1002-W
CN0010
CN0100
Si
O
O
OSi
Si
Si(CH3)
3
(CH2)
7CH
3
Si
O
O
OHSi
Si (CH2)
3CN
Cle
anert
SP
E
093
Particle Characteristics
Based on silica; Average particle diameter: 50μm; Average pore size: 60Å;
Volume of pore: 0.8cm2/g;Specific surface area: 480m2/g
Material Sorbent Vol Tubes/box Cat. Number
Cleanert NH2
Ordering Information
100mg
200mg
500mg
500mg
1g
50mg/well
100mg/well
10g
100g
100
50
50
30
30
96-wellplate
96-wellplate
1mL
3mL
3mL
6mL
6mL
2mL
2mL
Particle Characteristics
Based on silica; Average particle diameter: 50μm; Average pore size: 60Å;
Volume of pore: 0.8cm2/g;Specific surface area: 480m2/g
Material Sorbent Vol Tubes/box Cat. Number
Cleanert PSA
Ordering Information
100mg
200mg
500mg
500mg
1g
50mg/well
100mg/well
10g
100g
100
50
50
30
30
96-wellplate
96-wellplate
1mL
3mL
3mL
6mL
6mL
2mL
2mL
NH2 SPE products have silica based sorbents bonded with aminopropyl funtional group. This
sorbent can be used in either normal phase or reverse phase mode. It retains the analytes
either by a polar adsorption (from non-polar solution) or by weak anion exchange (from
aqueous solution). pKa=9.8.
Description
PSA SPE is similar to Cleanert-NH2. PSA has two amino groups with pKa=10.1 and 10.9
respectively. This sorbent is an anion exchanger slightly stronger than NH2. It can be used
for the extraction of metal ions by chelating interactions. It is also commonly used to remove
organic acids, pigments an metal ions from organic samples such as vegetables and fruits.
Description
NH2 (Aminopropyl)
PSA (N-aminoethyl) Aminopropyl
Si
O
O
Si OH
Si (CH2)
3NH
2
NH1001
NH2003
NH5003
NH5006
NH0006
NH0502-W
NH1002-W
NH0010
NH0100
PA1001
PA2003
PA5003
PA5006
PA0006
PA0502-W
PA1002-W
PA0010
PA0100
Si
Si
O
O
OH
Si (CH2)
3NH
2(CH
2)
2NH
2
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BETTER SOLUTION FOR CHROMATOGRAPHY
Cle
anert
SP
E
094
SAX SPE products have silica based sorbents bonded with a quaternary amine. This strong
anion exchanger is used to extract compounds capable of carrying a negative charge from
both aqueous and non-aqueous solutions. They are ideally suit to extraction of weak acids
and desalting of biological macromolecules, like BondElute-SAX.
Description
SAX (Strong Anion Exchanger)
Particle Characteristics
Based on silica; C%: 9-10%; Average particle diameter: 50μm; Average pore size: 60Å;
Volume of pore: 0.8cm2/g;Specific surface area: 480m2/g The ion exchange degree: 0.5meq/g.
Material Sorbent Vol Tubes/box Cat. Number
Ordering Information
100mg
200mg
500mg
500mg
1g
50mg/well
100mg/well
10g
100g
100
50
50
30
30
96-wellplate
96-wellplate
1mL
3mL
3mL
6mL
6mL
2mL
2mL
COOH SPE products consist of a propane carboxylic acid on the inner silica surface. The
pKa of the carboxylic acid group is approximately 3.8. It is a useful orbent for quaternary
ammonium salt and other strong cations.
Description
COOH (Weak Cation Exchanger)
Particle Characteristics
Based on silica; C%: 5-6%; Average particle diameter: 50μm; Average pore size: 60Å;
Volume of pore: 0.8cm2/g;Specific surface area: 480m2/g
Material Sorbent Vol Tubes/box Cat. Number
Ordering Information
100mg
200mg
500mg
500mg
1g
50mg/well
100mg/well
10g
100g
100
50
50
30
30
96-wellplate
96-wellplate
1mL
3mL
3mL
6mL
6mL
2mL
2mL
Cleanert SAX
SA1001
SA2003
SA5003
SA5006
SA0006
SA0502-W
SA1002-W
SA0010
SA0100
Si O
O
OHSi
Si (CH2)
3N+(CH
3)
3Cl-
Cleanert COOH
CH1001
CH2003
CH5003
CH5006
CH0006
CH0502-W
CH1002-W
CH0010
CH0100
Si
O
Si
O
OH
Si (CH2)
3COOH
Cle
anert
SP
E
095
PRS (Propane Sulfonic Acid)
Particle Characteristics
Based on silica; Average particle diameter: 50μm; Average pore size: 60Å;
Volume of pore: 0.8cm2/g;Specific surface area: 480m2/g The ion exchange degree: 0.3meq/g.
Material Sorbent Vol Tubes/box Cat. Number
Ordering Information
100mg
200mg
500mg
500mg
1g
50mg/well
100mg/well
10g
100g
100
50
50
30
30
96-wellplate
96-wellplate
1mL
3mL
3mL
6mL
6mL
2mL
2mL
SCX (Strong Cation Exchanger)
Particle Characteristics
Based on silica; Average particle diameter: 50μm; Average pore size: 60Å;
Volume of pore: 0.8cm2/g;Specific surface area: 480m2/g The ion exchange degree: 0.5meq/g.
Material Sorbent Vol Tubes/box Cat. Number
Ordering Information
100mg
200mg
500mg
500mg
1g
50mg/well
100mg/well
10g
100g
100
50
50
30
30
96-wellplate
96-wellplate
1mL
3mL
3mL
6mL
6mL
2mL
2mL
PRS SPE sorbent is a silica gel based strong cation exchanger. This sorbent, consisting of a
propane sulfonic acid, has slightly less exchange caoability than SCX. It can be applied to
the extraction of weak cations, such as pyridine, with high recovery.
Description
SCX products are strong cation exchangers based on silica gel, with benzene sulfonic acid.
The sorbent is used to extract positively charged basic compounds or remove the salt from
biological samples. It can also be mixed with C18 sorbent to extract the organic bases.
Description
Si
Si
Si
O
O
OH
(CH2)
3SO
3H
Cleanert PRS
PR1001
PR2003
PR5003
PR5006
PR0006
PR0502-W
PR1002-W
PR0010
PR0100
Si
O
Si
O Si
OH
(CH2)
2SO
3-H+
Cleanert SCX
SC1001
SC2003
SC5003
SC5006
SC0006
SC0502-W
SC1002-W
SC0010
SC0100
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BETTER SOLUTION FOR CHROMATOGRAPHY
Cle
anert
SP
E
096
Silica SPE product has unbonded, activated irregular silica as the sorbent. This sorbent
exhibits high polar interaction and is used for the extraction of weak-polar or non-polar
compound, such as oil. In addition, the silanol groups are ionzable at intermediate pH, so it
can be used as a weak cation exchanger.
Description
Particle Characteristics
Based on silica; Average particle diameter: 50μm; Average pore size: 60Å;
Volume of pore: 0.8cm2/g;Specific surface area: 480m2/g
Material Sorbent Vol Tubes/box Cat. Number
Ordering Information
100mg
200mg
500mg
500mg
1g
50mg/well
100mg/well
10g
100g
100
50
50
30
30
96-wellplate
96-wellplate
1mL
3mL
3mL
6mL
6mL
2mL
2mL
Silica based dihydroxy SPE. It is used to extract polar analytes from non-polar solutions. It is
a neutral sorbent and extracts compounds by forming hydrogen bonding or polar-polar
interaction. As an example, it can be used to extract THC.
Description
Silica
Diol
Particle Characteristics
Based on silica; C%: 5-6%; Average particle diameter: 50μm; Average pore size: 60Å;
Volume of pore: 0.8cm2/g;Specific surface area: 480m2/g
Material Sorbent Vol Tubes/box Cat. Number
Ordering Information
100mg
200mg
500mg
500mg
1g
50mg/well
100mg/well
10g
100g
100
50
50
30
30
96-wellplate
96-wellplate
1mL
3mL
3mL
6mL
6mL
2mL
2mL
Cleanert Silica
SI1001
SI2003
SI5003
SI5006
SI0006
SI0502-W
SI1002-W
SI0010
SI0100
Si
Si
O
OH
OH
Cleanert Diol
DI1001
DI2003
DI5003
DI5006
DI0006
DI0502-W
DI1002-W
DI0010
DI0100
Si CH2CH
2CH
2OCH
2CH CH
2
OHOH
Cle
aner
t SP
E
097
Florisil (Magnesia Silica)
Particle Characteristics
Adsorption sorbents; Average particle diameter: 80-100μm (40-60μm optional);
Average pore size: 80Å; Specific surface area: 291m2/g
Material Sorbent Vol Tubes/box Cat. Number
Ordering Information
100mg
200mg
500mg
500mg
1g
50mg/well
100mg/well
10g
100g
100
50
50
30
30
96-wellplate
96-wellplate
1mL
3mL
3mL
6mL
6mL
2mL
2mL
Particle Characteristics
Adsorption sorbents; Average particle size: 120-400 mesh.
Material Sorbent Vol Tubes/box Cat. Number
Ordering Information
100mg
200mg
500mg
500mg
1g
50mg/well
100mg/well
10g
100g
100
50
50
30
30
96-wellplate
96-wellplate
1mL
3mL
3mL
6mL
6mL
2mL
2mL
PestiCarb is made of graphitized carbon by a distinct surface modification process, and has
been used for sample cleanup in pesticide residues in plant or animal tissues. This sorbent
can effectively reduce the background noise and increase the sensitivity, the functions of
which are similar to Supelco Envicarb.
Description
PestiCarb is made of graphitized carbon by a distinct surface modification process, and has
been used for sample cleanup in pesticide residues in plants or animal tissues. This sorbent
can effectively reduce the background
noise and increase the sensitivity, the functions of which are similar to Supelco Envicarb.
Description
PestiCarb (Graphitized Carbon)
Non-silica Adsorption Sorbent
Florisil
(Magnesia Silica)
Cleanert Florisil
FS1001
FS2003
FS5003
FS5006
FS0006
FS0502-W
FS1002-W
FS0010
FS0100
Graphitized Carbon
Cleanert
PestiCarb
PC1001
PC2003
PC5003
PC5006
PC0006
PC0502-W
PC1002-W
PC0010
PC0100
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umns
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BETTER SOLUTION FOR CHROMATOGRAPHY
Cle
aner
t SP
E
098
Alumina N sorbents(pH=7.5) can adsorb molecules by interaction with the aluminum metal
center. The neutralized surface allows interaction with compounds whose heteroatoms are
electronegative(e.g.N,S,P) or with an electron-rich ,highly aromatic structure.
Description
Particle Characteristics
Adsorption sorbents;
Average particle size: 150 mesh; Average pore size: 58Å;
Material Sorbent Vol Tubes/box Cat. Number
Ordering Information
100mg
200mg
500mg
500mg
1g
50mg/well
100mg/well
10g
100g
100
50
50
30
30
96-wellplate
96-wellplate
1mL
3mL
3mL
6mL
6mL
2mL
2mL
Alumina N (Aluminium Oxide;Neutral)
Aluminium Oxide
(Neutral)
Cleanert
Alumina N
AL1001-N
AL2003-N
AL5003-N
AL5006-N
AL0006-N
AL0502-N-W
AL1002-N-W
AL0010-N
AL0100-N
Alumina A sorbents(pH=4.5) can be used as strong polar absorbents or mild cation exchang-
ers. This sorbent is processed with a special deactivation procedure which ensures high
analytes recovery.
Description
Alumina A (Aluminium Oxide;Acidic)
Particle Characteristics
Adsorption sorbents;
Average particle size: 150 mesh; Average pore size: 58Å;
Material Sorbent Vol Tubes/box Cat. Number
Ordering Information
100mg
200mg
500mg
500mg
1g
50mg/well
100mg/well
10g
100g
100
50
50
30
30
96-wellplate
96-wellplate
1mL
3mL
3mL
6mL
6mL
2mL
2mL
Aluminium Oxide(Acidic)
Cleanert
Alumina A
AL1001-A
AL2003-A
AL5003-A
AL5006-A
AL0006-A
AL0502-A-W
AL1002-A-W
AL0010-A
AL0100-A
Cle
aner
t SP
E
099
Particle Characteristics
Adsorption sorbents;
Average particle size: 150 mesh; Average pore size: 58Å.
Material Sorbent Vol Tubes/box Cat. Number
Ordering Information
100mg
200mg
500mg
500mg
1g
50mg/well
100mg/well
10g
100g
100
50
50
30
30
96-wellplate
96-wellplate
1mL
3mL
3mL
6mL
6mL
2mL
2mL
PestiCarb/NH2
Particle Characteristics
See Pesticarb and Cleanert NH2
Material Sorbent Vol Tubes/box Cat. Number
Ordering Information
Alumina B products(pH=10) can be used to remove organic acids and phenols in the sample
matrix. They have been pre-treated by special deactivation to ensure high analytes recovery.
Description
PestiCarb/NH2 SPE column is packed of 500mg PestiCarb and 500mg NH2.It has been used
wildly in analysis of pesticides residue, esp. for the JapanesePositive List System. It can be
used in pesticide residue analysis, coloring matter,fatty acid and hydroxybenzene.
Description
Alumina B (Aluminium Oxide; Basic)
Aluminium Oxide
(Basic)
PestiCarb/NH2
Cleanert PestiCarb/NH2 500mg/500mg 6ml 30 PN0006
Cleanert
Alumina B
AL1001-B
AL2003-B
AL5003-B
AL5006-B
AL0006-B
AL0502-B-W
AL1002-B-W
AL0010-B
AL0100-B
Mixed and Layered Phases
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Cle
aner
t SP
E
100
Mixed mode SPE based on silica of C8 and strong cation-exchange. It is usually used
for the extraction of basic drugs from urine or blood
Description
C8/SCX
Particle Characteristics
See C8 and SCX
Material Sorbent Vol Tubes/box Cat. Number
Ordering Information
50
50
50
30
30
96-wellplate
96-wellplate
3mL
3mL
3mL
6mL
6mL
2mL
2mL
HXN is also made of polydivinylbenzene having surface modified with vinylpyrrol-
idone. This sorbent is specially designed to extract sulfonyl ureas from water and soil
at ppb level. It is less polar than PEP and can also be used to extract, enrich and clean
up samples from mid polar to high polar compounds.
Description
HXN(Mid Polar Polymers Specially for Sulfonyl Urea Samples)
Particle Characteristics
Based on polystyrene/divinylbenzene;Average particle diameter: 40μm;
Average pore size: 70Å; Volume of pore: 1.2cm2/g; Specific surface area: 600m2/g
Material Sorbent Vol Tubes/box Cat. Number
Ordering Information
C8/SCX
HXN(mid polar polymers specially
for sulfonyl urea samples)
Specialized Phases
Cleanert
C8/SCX
CS0503
CS1303
CS3003
CS5006
CS0006
CS0502-W
CS1002-W
CS0010
CS0100
50mg
130mg
300mg
500mg
1g
50mg/well
100mg/well
10g
100g
30mg
60mg
100mg
200mg
500mg
30mg/well
50mg/well
10g
100g
Cleanert HXN
100
50
50
30
30
96-wellplate
96-wellplate
HX0301
HX0603
HX1003
HX2006
HX5006
HX0302-W
HX0502-W
HX0010
HX0100
1mL
3mL
3mL
6mL
6mL
2mL
2mL
Cle
anert
SP
E
101
SUL-5(Specific Columns for Sulfonamides)
Particle Characteristics
Adsorption sorbents.
Ordering Information
SUL-5 specific columns for sulfonamides is specially designed for the extraction
of five sulfonamides(SM2,SMM,SMZ,SDM.SQ) in pork.
Description
SUL-5
(specific columns for sulfonamides)
Material Sorbent Vol Tubes/box Cat. Number
Cleanert SUL-5 2g 10ml 100 SUL-5
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BETTER SOLUTION FOR CHROMATOGRAPHY
Cle
anert
SP
E
102
Cleanert Pre-IC:
Sample Clean-up Cartridges for the Ion Chromatography
It is a layered cartridge that contains IC- Ag resin and IC- H resin.
It is a layered cartridge that contains IC-Ba resin, IC- Ag resin and IC- H resin.
Part No.
IC-RP
IC-Ag/H
IC-Ba
IC-Na
IC-P
IC-M
IC-Ba/Ag/H
IC-Ag
IC-H
IC-A
The type of resin
divinylbenzene
resin
styrene-based,
sulfonic acid ,
cation-exchange
resin in the
barium form.
16% cross-linked,
styrene-based,
sulfonic acid ,
cation-exchange
resin in the
sodium form.
polyvinylpyrrolidone
(PVP) polymer resin
iminodiacetate
resin in the
ammonium form
16% cross-linked,
styrene-based,
sulfonic acid,
cation-exchange
resin in the silver
form.
16% cross-linked,
styrene-based,
sulfonic acid ,
cation-exchange
resin in the
hydrogen form
16% cross-linked,
styrene-based,
anion-exchange
resin in the
bicarbonate form.
Average
particle size
40µm
40µm
40µm
40µm
40µm
40µm
40µm
40µm
Sepcification
10 pk
10 pk
10 pk
10 pk
10 pk
10 pk
10 pk
10 pk
10 pk
10 pk
Capacity
300mg/1cc
cartridge
2.0-2.2meq
/1cc cartridge
2.0-2.2meq
/1cc cartridge
350mg/1cc
cartridge
0.4meq/1cc
cartridge
2.0-2.2meq
/1cc cartridge
2.0-2.2meq
/1cc cartridge
0.7meq/1cc
cartridge
Application
To remove substances such as aromatic dyes, some
aromatic carboxylic acids, hydrocarbons, and
surfactants from sample matrices
To remove SO4
2-.For reproducible, quantitative
determinations in low-ionic-strength samples, these
cartridges should be activated with a chloride-based
activating solution. Then the added chloride need to
be removed.
To remove high levels of alkaline earths and transition
metals from sample matrices without acidifying the
sample. This ensures good recovery of acid labile
analytes such as nitrite.
To remove the phenolic fraction of humic acids,
tannic acids, lignins, anthocyanins, and azodyes
from samples.
To remove transition metals and matrix elimination
of alkali and alkaline earth metals.
To remove chloride, bromide, and iodide from sample
matrices. An IC- H cartridge should be used after the
IC- Ag cartridge to remove dissolved Ag.
To remove high levels of alkaline earths and transition
metals from sample matrices and in the neutralization
of highly alkaline samples such as sodium hydroxide
or sodium carbonate.
To remove anion contaminant and neutralize the
strongly acidic sample solution.
Description
The sample preparation is necessary not only for HPLC but also for IC.
The demands for sample preparation of IC led to a development of a new SPE product family, which we named ”Cleanert Pre-IC”, and they
can perform excellently for sample preparation of IC thanks to their sorbent of high capacity and a good flow property
Cle
anert
SP
E
103
There are many factors influenced the establishment of SPE, and the following are the four main ones which must
be considered:
The guide on this page briefly outlines the decision making process required to choose a suitable extraction mechanism.
1) The Selection of Sorbent Retention Mechanism
The Development of SPE Method
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Cle
anert
SP
E
104
The guide on this page briefly outlines the decision making process required to choose a suitable sorbent.
For the normal phase and reversed phase SPE cartridges, the weight of sample can not be exceed the 5% of cartridges. For the ion-exchange
mode, the capacity of the ion-exchange must be considered.
The table below is the capability and eluted parameter of SPE:
2) The Selection of SPE Columns
Sample
Organic Aqueous
Dissolved in
methanol
Dissolved in
hethane
Normal Phase
(adsorption)
Cation Reversed
Phase
Reversed
Phase
Anion Reversed
Phase
Normal phase
C18,C8,CN,
PEP,PS,HXNNH2, Diol
Silica,
Florisil SCX SAX
Ionizable Non-ionizable
Specification Quality of Loading Sample The Minimum Volume of Elution
50mg/1mL
100mg/1mL
200mg/3mL
500mg/6mL
1g/6mL
2.5mg
5mg
10mg
25mg
50mg
125μL
250μL
500μL
1.2mL
2.4mL
C18,C8,PEP,PS,HXNC18,C8,PEP,PS,HXN
The selection of column packaging and the parameter of sample loading and elution
Cle
anert
SP
E
105
3) The Selection of Ideal Elution Solvent
The elution strength of reversed solvent
The elution strength of normal solvent
Hexane
Iso-octyl-alcohol
Tetrachloromethane
Chloroform
Dichloromethane
Tetrahydrofuran
Aether
Ethyl acetate
Acetone
Acetonitrile
Isopropanol
Methanol
Water
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BETTER SOLUTION FOR CHROMATOGRAPHY
Cle
anert
SP
E
106
SPE Columns
Cleanert PAX, 30mg/1mL
SPE Procedure
1) Condition: 1mL methanol
2) Equilibration: 1mL water
3) Sample loading: 500 μL standard sample(8% NH3•H2O)
4) Washing: 1 mL 8% NH3•H2O
5) Elution: 5% Formic acid in methanol
HPLC Conditions
Applications
Determination of Oleic acid and its Metabolites in Human Plasm
Mobile phase: ACN: 3mmol/L ammonium acetate=85 15
HPLC column: Venusil ASB C18 Column 2.1×50mm
MS Conditions
MS: Qtrap 3200
Ion Source: ESI
Scan Mode: Negative mode
Single ions monitor (SIM): Oleic acid m/z 281.2; Internal standard substances C17 m/z 269.2
Multiple reactive monitor (MRM): Metabolite of oleic acid m/z 315.2 →141.1
O
HO
XIC of+MRM(7 pairs):315.2/127.1 amu from Sample 209(126) of 0712DHSA.wiff(Turbo Spray)
XIC of+MRM(7 pairs):315.2/141.1 amu from Sample 209(126) of 0712DHSA.wiff(Turbo Spray)
Max 2830.0 cps
Max 4660.0 cps
Inte
nsity.
cp
sIn
ten
sity.
cp
s
Time.min
Time.min
7.8e5
7.0e5
6.0e5
5.0e5
4.0e5
3.0e5
2.0e5
1.0e5
0.0e51 2 3 4 5 6 7 8 9 10 11
3.12
7.8e5
4000
3000
2000
1000
01 2 3 4 5 6 7 8 9 10 11
3.12
3.14
3.123.35
Cle
anert
SP
E
107
SPE Columns
Cleanert PCX, 100mg/1mL
SPE Procedure
1) Condition: 1mL methanol
2) Equilibration: 1mL water
3) Sample loading: 500 μL standard sample(8% NH3•H2O)
4) Washing: 1 mL 8% NH3•H2O
5) Elution: 5% Formic acid in methanol
HPLC Conditions
Determination of Pseudoephedrine Hydrochloride and its Metabolites in Plasm
Mobile phase: A:1% Formic acid;B: Methanol
Venusil ASB C18 Column 2.1×50mm
Flow rate: 0.5 mL/min
MS Conditions
MS: Qtrap 3200
Ion Source: ESI
Scan Mode: Positive mode
Multiple reactive monitor (MRM): Pseudoephedrine Hydrochloride m/z 166.0 →148.1
Internal standard substances: Lidocaine m/z 235.3→86.1
Table of Gradient Elution Program
Time(min) Mobile phase A Mobile phase B
0.00
2.00
3.00
3.50
80
5
5
80
20
95
95
20
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Cl
HC N N O
O
OH
+2HCL
XIC of+MRM(5 pairs):235.2/86.1 amu from Sample 4(Sample004) of lhl_20080124_wmhj_grd.wiff(Turbo Spray).Smoothed
XIC of+MRM(5 pairs):166.2/148.1 amu from Sample 4(Sample004) of lhl_20080124_wmhj_grd.wiff(Turbo Spray).Smoothed,Smoothed
Max 2.9e5 cps
Max 4.1e5 cps
Inte
nsity.
cp
sIn
ten
sity.
cp
s
2.122.8e5
4.0e5
3.5e5
3.0e5
2.5e5
2.0e5
1.5e5
1.0e5
0.5e5
0.0e5
2.6e5
2.4e5
2.2e5
2.0e5
1.8e5
1.6e5
1.4e5
1.2e5
1.0e5
0.8e5
0.6e5
0.4e5
0.2e5
0.0e50.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2
1.47
0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 1.8 2.0 2.2 2.4 2.6 2.8 3.0 3.2
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BETTER SOLUTION FOR CHROMATOGRAPHY
Cle
anert
SP
E
108
Description
Sample: 50ng/mL in human serum (dilute two times by pure water)
SPE column: Cleanert C18, 200mg/3mL
The Recovery of Evodiamine and Rutecarpine in Human Serum
SPE Procedure
1) Column activation: 2mL MeOH; 2mL H2O
2) Sample loading: 2mL (diluted) sample, at flow rate of 0.5mL/min
3) Washing: 1mL × 2H2O; drying using N
2.
4) Elution: 1mL× 2 Ethyl acetate
5) Evaporation: evaporate solvent under N2 at 40
6) Reconstitution: with 1mL MeOH/water (85/15) and filtrated using 0.45μm membrane
LC-MS Conditions
Determination of Evodiamine and Rutecarpine in Human Serum by LC-MS
NH
N
N
O
Me
H
NH
N
N
O
Evodiamine Rutecarpine
Agilent 1100/Trap XCT
Column: Venusil XBP-C18, 4.6 × 150mm, 5μm
Mobile phase: methanol/ammonium formate = 85/15
Flow: 0.5mL/min; Temp: 25 ; Inj: 10μL; MS: ESI
SPE Columns
Cleanert Alumina B; Cleanert SCX
SPE Procedure
1) Extract the ground fish with ACN; evaporate ACN; dissolve the
residue in CH2Cl
2.
2) Put Alumina B column on the top of SCX; wash both with 5mL ACN
3) Load the sample (2-3mL) into Alumina B
4) Wash both columns with 5mL ACN
5) Throw away Alumina B, elute the sample from SCX column using
4mL ACN/ammonium acetate(0.1M, pH 4.5)=1:1
HPLC Conditions
Extraction and Analysis of Malachite Green in Salmon
Column: Venusil XBP-C18, 4.6 × 150mm, 5μm
Mobile phase: ACN/ammonium acetate(0.1M, pH 4.5)=80:20;
Flow rate: 2mL/min; Temperature: 35 ; Detection: UV 588nm
Analyte Concentration (ng/mL) Mean Recovery(%) R.S.D.(%)
Evodiamine
Rutecarpine
98.1
97.1
91.5
90.5
5.24
2.32
2.48
3.90
520
52
510
51
H3C
CH3 CH3
ClCH3
N N
0 0.5 1 1.5 2 2.5 3
mAU
0.25
0.2
0.15
0.1
0.05
0
-0.05
-01
Cle
anert
SP
E
109
SPE Column
Cleanert C18/SCX (500 mg/3 mL, prototype)
Result
Recovery = 80-90% for 2 μg/kg dose, RSD<20%
SPE Method
1) Activate the column with 2 mL MeOH, 2 mL 10 mmol HCl aqueous solution;
2) Load 5 mL sample at the flow rate 1 mL/min
3) Rinse the column with 5 mL water, 5 mL MeOH, dry the column
4) Elute analyte with 5 mL 4% ammonia in MeOH.
5) Evaporate the solvent and reconstitute the sample in 1 mL 1% formic acid/MeOH = 9 : 1
Determination of Clenbuterol in Urine Samples by Mixed- phase SPE and LC-MS
SPE Column
Cleanert Florisil 1000mg/6mL strong cations.
LC-MS Detection
SPE Method
1) Condition: 5ml methanol, 5ml ether.
2) Sample loading: 5mL sample
3) Wash: 5ml ether
4) Elution: methanol/ether=7/3
The Analysis of Trace Chloramphenicol
13.00 13.50 14.00 14.50 15.00 15.50 16.00 16.50 17.00 17.50 18.00 18.50
2000
4000
6000
8000
10000
12000
14000TIC: 03081801.D
13. 98
14. 56
15. 91
17. 55
TAP
FF
CAP
m-CAP
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BETTER SOLUTION FOR CHROMATOGRAPHY
Cle
anert
SP
E
110
Materials
1) SPE column: Cleanert PEP, 4-nitrophenol
2) Seven kinds of hydroxybenzene: phenol, Cresol, 4-nitrophenol, 2-Chlorophenol, 2,4-Dichlorophenol, 2,4,6-Trichlorophenol, Pentachloro
phenol
SPE Procedure
1) Column Condition: 5mL Methyl tert-butyl ether/methanol(V/V=10:90) , 5mL/min;
2) Equilibration: 5mL water
3) Sample loading: 1L sample, 10mL/min
4) Washing: 10mL water, then drying using N2 for 20mins
5) Elution: 2mL Methyl tert-butyl ether
HPLC Conditions
Detection of Seven Hydroxybenzenes in Water (PEP)
Mobile phase: A: 1% acetic acid, B: 1% acetic acid and methanol
HPLC column: AQ C18 (4.6 × 150mm, 5μm); UV Detector
Time Mobile Phase Flow Rate (mL/min) UV (nm)
0-15min
15-30min
1
1.8
275
295
A:B=50:50
A:B=15:85
Chart 1. The chromatogram of seven kinds of hydroxybenzene
1 phenol; 2 4-nitrophenol; 3 Cresol; 4 2-Chlorophenol ; 5 2,4-Dichlorophenol; 6 2,4,6-Trichlorophenol ; 7 Pentachlorophenol
2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 22.5 25.0 27.5 30.0
0.004
0.002
0.000
-0.002
1
2
3
4
5 6
7
Cle
anert
SP
E
111
SPE Method
Result of Recoveries of Five Kinds of Sulfonamides in Pork
1) Condition: 5ml 95% acetonitrile
2) Load: 3mL sample which is dissolved by 95% acetonitrile
3) Wash: 5mL 95% acetonitrile
4) Elute:10mL 70% acetonitrile
The Extraction of Five Kinds of Sulfonamides in Pork
Sample
2-nitroaniline and 4-nitroaniline in 0.1% ammonia aqueous solution.
SPE Procedure
1) Activation, 3mL methanol, 3mL DI water
2) Loading, 3mL sample
3) Washing: 1mL 0.1% ammonia aqueous solution
4) Elution: 3mL methanol/1%formic acid = 95/5.
Drying with N2 and reconstitute the residue using 1mL methanol.
HPLC conditions
Venusil AQ C18 HPLC column, 4.6x250mm; Mobile phase: Methanol/1%formic acid = 10/90
Cleanert PEP Application Testing Examples
Results
0
20
40
60
80
100
SM2 SMM SMZ SDM SQ
Concentration (100μg/kg)
0
20
40
60
80
100
SM2 SMM SMZ SDM SQ
Concentration(200μg/kg)
Nitroanilines
Sample 4-nitroaniline Concentr. (ppm) Peak Area(avg) Std Peak Area(avg) PEP Recovery (%) PEP
a
b
c
a
b
c
296740.5
28374.5
2830.5
464657.5
45113.5
3489.5
270652
31517
2947
456445
46537
4234
91
111
104
98
103
121
20
2
0.2
20
2
0.2
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BETTER SOLUTION FOR CHROMATOGRAPHY
Cle
anert
SP
E
112
Sample
Aniline and N, N-dimethylaniline in DI water solution.
SPE Procedure
1) Activation, 3mL methanol, 3mL DI water
2) Loading, 3mL sample
3) Washing: 1mL water
4) Elution: 3mL methanol/water = 95/5
Drying with N2 and reconstitute the sample using 1mL methanol.
HPLC conditions
Venusil AQ-C18 column, 4.6 × 250mm, 5μm Mobile phase: methanol/water = 50/50
Results
Aniline and N, N-dimethylaniline
Sample 4-nitroaniline Concentr. (ppm) Peak Area(avg) Std Peak Area(avg) PEP Recovery (%) PEP
a
b
c
a
b
c
d
360251
36686
3563
1418380
174334
19483
2032
213975
20672
2870
1398427
159897
18386
1841
60
56
81
98
92
94
91
200
20
2
200
20
2
0.2
Cle
anert
SP
E
113
SPE Procedure
1) Condition and equilibrate: 2mL methanol, 2mL water
2) Load: 4mL sample(diluted by 0.2% acetic acid)
3) Wash: 1mL water
4) Elute: 4mL methanol
HPLC conditions
Praziquantel st
methanol: water=70 : 30, UV=263nm, HPLC column: Venusil AQ C18, 4.6 × 150mm, 5μm
Praziquantel
min0 1 2 3 4 5 6 7
mAU
0
0.5
1
1.5
2
5.7
32
SPE Procedure
1) Condition and equilibrate: 1mL dichloromethane, 1mL water
2) Load: 4mL sample (diluted by 5mL 50% ACN and water and 10mM K2HPO
4)
3) Elute: 4mL methanol
HPLC conditions
Promosil C18, 20μL, ACN: H2O=60/40, UV=235nm
Gliclazide
0 1 2 3 4 5
350
300
250
200
150
100
50
0
mAU
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BETTER SOLUTION FOR CHROMATOGRAPHY
Cle
anert
SP
E
114
Cross-reference Table
Cleanert
C18
ENVI-18
LC-C18
Bond Elut
C18
AccuBOND
C18
Sep-pak
C18
Cleanert
C18-N
Bond Elut
C18-OH
AccuBOND
C8
Cleanert
C8ENVI-8
Bond Elut
C8
Sep-pak
C8
AccuBOND
CN
Cleanert
CNLC-CN
Bond Elut
CN
Sep-pak
CN
AccuBOND
NH2
Cleanert
NH2
LC-NH2Bond Elut
NH2
Sep-pak
NH2
AccuBOND
Silica
Cleanert
SilicaLC-Silica
Bond Elut
Silica
Sep-pak
Silica
Sep-pak
Diol
Oasis
HLB
Cleanert
COOHLC-WCX
Bond Elut
CBA
Cleanert
PRS
Bond Elut
PRS
Cleanert
PEP
Bond Elut
Plexa
Oasis
MAX
Cleanert
PAX
Oasis
MCX
Cleanert
PCX
Cleanert
HXN
AccuBOND
SAX
Cleanert
SAXLC-SAX
Bond Elut
SAX
AccuBOND
SCX
Cleanert
SCXLC-SCX
Bond Elut
SCX
AccuBOND
Diol
Cleanert
DiolLC-Diol
Bond Elut
Diol
Cleanert
PSA
Bond Elut
PSA
Agela Waters Supelco Aglient Varian
AccuBOND
ENV PS-DVB
Cleanert
PS
ODS C18
(Endcapped)
ODS C18-N
(Non-endcapped)
C8
CN
Cyanopropyl
NH2
Aminopropyl
Silica
COOH
(Weak cation exchanger)
PRS
(Propane sulfonic acid)
PEP
(Polar polymers)
PAX
PCX
HXN
SAX
(Strong anion exchanger)
SCX
(Strong cation exchanger)
Diol
PSA
(N-aminoethyl Aminopropyl)
PS ENVI-Chrom P
Purification Products
Preparative HPLC Columns
Features
Ordering Information
Flash Chromatography Media and Columns
Flash Chromatography System
116
116
118
120
125
AgelaAgelaTechnologies
BETTER SOLUTION FOR CHROMATOGRAPHY
Purific
atio
n
116
0 2 4 6 8 10 12 min
4.6mm 0.7mL / min 2 μL
0 2 4 6 8 10 12 min
20.0mm 14.87mL / min 42.4 μL
0 2 4 6 8 10 12 min
30.0mm 29.77mL / min 170 μL
0 2 4 6 8 10 12 min
50.8mm 85.37mL / min 488 μL
Features
Preparative HPLC Columns
Agela Technologies offers a full line of preparative HPLC columns to meet a variety of application needs.
Our preparative columns feature:
1.Great scalability
2.Excellent bed stability
3.High loading capacity
4.Broad solvent compatibility,from 100% aqueous to 100% organic solvents (Unisol C18, Unisol Amide, and Venusil ASB-C18)
5.Broad pH range,1-12 (Venusil Durashell)
6.Unique selectivity (Unisol Amide and Venusil ASB-C18)
Unisol-Amide: an unique phase from Agela Technologies that offers a good alternative selectivity to reverse phase columns and an excellent
solution for purifying highly polar compounds.
Durashell RP: High pH tolerance enables the use of high pH mobile phase, which results in great improvement of peak shape and loadability
of basic compounds.
Column Unisol Amiide , 4.6 x 250 mm, 5μm
Mobile phase ACN / Water = 85/15~40/60 in 30 min
Detection UV 210nm
Scalability
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Purific
atio
n
Excellent Bed Stability
Venusil XBP-C18, 20 x 150 mm, 10 μm >1000 injection
Efficiency vs. Number of lnjections
Pla
te C
ou
nt
0 125 250 375 500 625 750 875 1000
Plate Count
9000
8000
7000
6000
5000
Number of lnjectionsUracil Phenol 4CINB Toluene
XXXXXX
X
X
X
X
Mass Load Comparison
Column: C18, 20 x 150 mm, 5μm
Sample: Diphenhydramine, 100 mg/mL in DMSO
Mobile phase: 10 mM ammonia: ACN=65: 35
Injection: 800μL
0 1 2 3 4 5 6 7 8 9 n
500
400
300
200
100
0
mAU
Brand 1
0 1 2 3 4 5 6 7 8 9 n
500
400
300
200
100
0
mAU
Brand 2
0 1 2 3 4 5 6 7 8 9 n
500
400
300
200
100
0
mAU
Brand 3
0 1 2 3 4 5 6 7 8 9 n
500
400
300
200
100
0
mAUVenusil XBP-C18
117
AgelaAgelaTechnologies
BETTER SOLUTION FOR CHROMATOGRAPHY
Pur
ifica
tion
118
Analytical Preparative
Purpose Purpose
Information about the composition Obtain pure components
Interested in most or all components Interested in one or a few components
Characteristics Characteristics
Small injection Large injection
Reverse phase preferred Normal phase may be preferred
1~5mm internal diameter 1~10cm internal diameter
2-5μm packing materials >5μm packing materials
HPLC pump <10mL/min HPLC pump ≥10mL/min
Injection usually trouble free May have difficulties for sample injection
Maximize detection sensitivity Minimize detection sensitivity
Sample solubility less important Sample solubility very important
Mobile phase volatility less important Mobile phase volatility very important; no-volatile additiveis prohibited.
Analytical/Preparative HPLC Comparison
Ordering Information
Pore Size: 100Å
Dimension
(mm)
Particle
(μm)
Venusil
XBP-C18
Venusil
XBP-C8
Venusil
Silica
Durashell RP Venusil
ASB-C18
Venusil
ASB-C8Type
Semi-preparative
Semi-preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Guard cartridge
Semi-preparative
Semi-preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Guard cartridge
10 150
10 250
20 50
20 150
20 250
30 100
30 150
30 250
20 10
10 150
10 250
20 50
20 150
20 250
30 100
30 150
30 250
50 150
50 250
20 10
5
5
5
5
5
5
5
5
5
10
10
10
10
10
10
10
10
10
10
10
VX951510-0
VX952510-0
VX950520-0
VX951520-0
VX952520-0
VX951030-0
VX951530-0
VX952530-0
VX950120-0
VX901510-0
VX902510-0
VX900520-0
VX901520-0
VX902520-0
VX901030-0
VX901530-0
VX902530-0
VX901550-0
VX902550-0
VX900120-0
VX851510-0
VX852510-0
VX850520-0
VX851520-0
VX852520-0
VX851030-0
VX851530-0
VX852530-0
VX850120-0
VX801510-0
VX802510-0
VX800520-0
VX801520-0
VX802520-0
VX801030-0
VX801530-0
VX802530-0
VX801550-0
VX802550-0
VX800120-0
VSi951510-0
VSi952510-0
VSi950520-0
VSi951520-0
VSi952520-0
VSi951030-0
VSi951530-0
VSi952530-0
VSi950120-0
VSi901510-0
VSi902510-0
VSi900520-0
VSi901520-0
VSi902520-0
VSi901030-0
VSi901530-0
VSi902530-0
VSi901550-0
VSi902550-0
VSi900120-0
VS951510-0
VS952510-0
VS950520-0
VS951520-0
VS952520-0
VS951030-0
VS951530-0
VS952530-0
VS950120-0
VS901510-0
VS902510-0
VS900520-0
VS901520-0
VS902520-0
VS901030-0
VS901530-0
VS902530-0
VS901550-0
VS902550-0
VS900120-0
VS851510-0
VS852510-0
VS850520-0
VS851520-0
VS852520-0
VS851030-0
VS851530-0
VS852530-0
VS850120-0
VS801510-0
VS802510-0
VS800520-0
VS801520-0
VS802520-0
VS801030-0
VS801530-0
VS802530-0
VS801550-0
VS802550-0
VS800120-0
DS951510-0
DS952510-0
DS950520-0
DS951520-0
DS952520-0
DS951030-0
DS951530-0
DS952530-0
DS950120-0
DS901510-0
DS902510-0
DS900520-0
DS901520-0
DS902520-0
DS901030-0
DS901530-0
DS902530-0
DS901550-0
DS902550-0
DS900120-0
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Pur
ifica
tion
119
Pore Size: 100Å
Unisol
C18
Unisol
AmideType
Semi-preparative
Semi-preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Guard cartridge
Semi-preparative
Semi-preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Preparative
Guard cartridge
Dimension
(mm)
10 150
10 250
20 50
20 150
20 250
30 100
30 150
30 250
20 10
10 150
10 250
20 50
20 150
20 250
30 100
30 150
30 250
50 150
50 250
20 10
Particle
(μm)
5
5
5
5
5
5
5
5
5
10
10
10
10
10
10
10
10
10
10
10
UO951510-0
UO952510-0
UO950520-0
UO951520-0
UO952520-0
UO951030-0
UO951530-0
UO952530-0
UO950120-0
UO901510-0
UO902510-0
UO900520-0
UO901520-0
UO902520-0
UO901030-0
UO901530-0
UO902530-0
UO901550-0
UO902550-0
UO900120-0
UA951510-0
UA952510-0
UA950520-0
UA951520-0
UA952520-0
UA951030-0
UA951530-0
UA952530-0
UA950120-0
UA901510-0
UA902510-0
UA900520-0
UA901520-0
UA902520-0
UA901030-0
UA901530-0
UA902530-0
UA901550-0
UA902550-0
UA900120-0
AgelaAgelaTechnologies
BETTER SOLUTION FOR CHROMATOGRAPHY
Purific
atio
n
120
Column Vol.(mL)Flash Column Size (g)
120 2000 176
80 1800 108
40 1100 54.6
20 900 30.5
12 700 17.9
4 500 8
Efficiency (phenylacetone)
Flash Chromatography Media and Columns
Column chromatography has been and will be continuously used as a major purification method for synthetic and natural products. Traditional
silica-based flash chromatography columns have many drawbacks: 1) high surface activity which causes instability of certain compounds, 2)
bad tailing or overly-long retention of basic compounds due to acid/base interaction or metal chelating effect, and 3) poor reproducibility.
Agela Technologies Inc developed a technology that can specially deactivate the silica media. Our columns offer significantly improved perfor-
mance and much less undesirable silica surface activities than the regular flash columns. Our columns may also provide alternative selectivity.
Flash CS Silica ColumnsUltra pure silica (specially washed with acid and DI water, narrow particle size control and water content control)
Column Specification
Surface Area: 460- 520m2/g ;Average Pore Size: 52- 68Å ;Surface pH: 6.3-7.2;Water content: 3.0-5.0% ;Average Particle Size: 40-60μm
Aniline Flash Column Tests Report
Column: Agela Cleanert CS (40 g), Brand I (40 g Si)
Mobile phase: Dichloromethane : methanol = 99 : 1
Detection: 254 nm
Temperature: Ambient
Flow rate: 20 mL/min
Rt: Brand I: 4.373 min; Agela: 4.090 min
Sample: Phenyl acetone , 4-aminobenzoicacid
Mobile phase: Hexane/ethylacetate (gradient)
Detection: 254 nm
Temperature: Ambient
Chromatography Comparison
Agela Cleanert CS Brand I
Pur
ifica
tion
121
HILIC Flash Chromatography
Following the success of Agela’s Venusil HILIC HPLC columns, we are now introducing HILIC phases for the Flash Chromatography applica-
tions. Agela’s HILIC columns are compatible with polar and aqueous solvents, such methanol, acetonitrile, and water, which makes the solvent
handling much easier compared to that for the normal phase separations. Significantly, because of the easy operation principle in using Agela’s
HILIC columns, these columns are designed to last. You will get a great return of the column investment at an already truly affordable price.
1. Solution for compounds that re not soluble in non- or low polar solvents (hexane, IPA, toluene, dichloromethane).
2. Solution for highly polar compounds which have too much retention on silica columns.
3. Solution for a mixture of compounds with a broad range of polarity (non-polar, semi-polar, and high polar) with alternative selectivity.
Flash CM Silica Columns
Chromatogram Comparison of Catechol on Different Silica
The following figures present a comparison of column performance for an Agela’s deactivated silica column vs an unmodified silica flash
column.
Test:
The silica to be tested was packed into a stainless steel column of
4.6 × 150mm and tested on a HPLC system
Conditions:
Sample: Catechol 100μg/mL in mobile phase
Mobile phase: dichloromethane/methanol = 98/2
Temperature: 30oC
Detection: 254nm
Injection: 5μL
Silica Characteristics:
Average Particle Size: 50μm
Average Pore Size: 60Å
Shape: Irregular
Surface Area: 500m2/g
Unmodifiedsilica
Generallydesactivated
silica (CM)
0 1 2 3 4 5 6 7 8 9
240220200180
160140120100
80
604020
0-20-40
4.4
07
5.1
49
3.0
90
0 1 2 3 4 5 6 7 8 9 10
400
350
300
250
200
150
100
50
0
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All HILIC phases are available for TLC, HPLC and Flash columns
Applications for HILIC columns are listed below:
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atio
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More important, you will get all the benefits at a truly affordable price, and may even lower the cost by multiple uses of the columns!
Column: 20g HILIC column
Sample: 10 mg of uridine in 3 mL hot water
Mobile phase: water
Detection: 254 nm
Flow rate: 12 mL/min
Sample: 100mg VC and 70mg VB2, direct solid loading
Mobile phase: 0-2min EtOAc; 2-22min MeOH(0-100%); 22-52min MeOH
Detection: 280 nm
Flow rate: 25 mL/min
Sample: 100mg VC and 70mg VB2, direct solid loading
Mobile phase: 0.1% TFA : ACN = 99 : 1
Detection: 280 nm
Flow rate: 25 mL/min
20g silica column 20g HILIC column
Quick Work-up CartridgeThey are specially designed to replace the conventional aqueous work up /liquid-liquid extraction process as a part of organic synthesis and
purification.They are the through-columns packed with wet resin and/or diatomite.
How to use :
1 a synthetic mixture is injected directly injected onto a column.
2 chose a solvent which is not immiscible with water to rinse the desired compounds out of column.
3 concentrate the collected solution
4 add the concentrated mixture to a flash column or a HPLC column for further separation.
Phase Size Type Cat. No.
Celite
C18 (RP)
HILIC
SCX (retaining amines)
SAX (retaining acids)
PEP (RP)
Catalyst(metal) remover
1mL
3mL
6mL
1mL
3mL
6mL
1mL
3mL
6mL
1mL
3mL
6mL
1mL
3mL
6mL
1mL
3mL
6mL
1mL
3mL
6mL
I
II
II
I
II
II
I
II
II
I
II
II
I
II
II
I
II
II
I
II
II
QWC001
QWC003
QWC006
QW9001
QW9003
QW9006
QWH001
QWH003
QWH006
QWS001
QWS003
QWS006
QWA001
QWA003
QWA006
QWP001
QWP003
QWP006
QWM001
QWM003
QWM006
Purific
atio
n
123
Ordering Information(Compatible with ISCO, Buchi and Biotage flash chromatography instruments)
CS: regular silica; CM: general deactivated, low metal and less retentive; NH2: aminopropyl; ODS: C18 reverse phase; HILIC: Hydrophilic
interaction column.
Quantity
(pk)Surface Type
Silica (CS)
Silica (CM)
Silica NH2
Silica ODS
HILIC
Silica Amount
(g)
4
12
20
40
80
120
330
800
1500
2500
4
12
20
40
80
120
330
800
4
12
20
40
80
120
330
800
4
12
20
40
80
120
330
800
4
12
20
40
80
120
330
800
Cat.
Number
CS140004-0
CS140012-0
CS140020-0
CS140040-0
CS140080-0
CS140120-0
CS140330-0
CS140800-0
CS141500-0
CS142500-0
CM140004-0
CM140012-0
CM140020-0
CM140040-0
CM140080-0
CM140120-0
CM140330-0
CM140800-0
NH140004-0
NH140012-0
NH140020-0
NH140040-0
NH140080-0
NH140120-0
NH140330-0
NH140800-0
OD140004-0
OD140012-0
OD140020-0
OD140040-0
OD140080-0
OD140120-0
OD140330-0
OD140800-0
CH140004-0
CH140012-0
CH140020-0
CH140040-0
CH140080-0
CH140120-0
CH140330-0
CS140800-0
20
20
20
10
5
5
1
1
1
1
20
20
20
10
5
5
1
1
20
20
20
10
5
5
1
1
20
20
20
10
5
5
1
1
20
20
10
10
5
5
1
1
Best Value
Guaranteed Product Quality
Innovation to Benefit CustomersOrdering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
AgelaAgelaTechnologies
BETTER SOLUTION FOR CHROMATOGRAPHY
Purific
atio
n
124
Bulk Silica
Cat. No.
CS605005-P
CS605025-P
CS605005-G
CS605025-G
CS605001-P
CS605002-P
CS605001-G
CS605002-G
CS608005-P
CS608025-P
CS608005-G
CS608025-G
CS608001-P
CS608002-P
CS608001-G
CS608002-G
Grade
P
P
G
G
P
P
G
G
P
P
G
G
P
P
G
G
Particle Size
40-60µm
40-60µm
40-60µm
40-60µm
40-60µm
40-60µm
40-60µm
40-60µm
70-90µm
70-90µm
70-90µm
70-90µm
70-90µm
70-90µm
70-90µm
70-90µm
Pack
5kg
25kg
5kg
25kg
1kg
2.5kg
1kg
2.5kg
5kg
25kg
5kg
25kg
1kg
2.5kg
1kg
2.5kg
Fritted Empty Cartridges for Filtration or Solid Sample Loading with Screw Caps
Volume (mL)
3
6
10
30
60
120
Cat. No.
SLC 0003
SLC 0006
SLC 0008
SLC 0024
SLC 0040
SLC 0120
Quantity (pk)
50
50
50
30
20
20
P: Reagent Grade; G: Tech.Grade
Piston Seals, Cartridge
10 mL
30 mL
50 mL
SLS 0008
SLS 0024
SLS 0040
Volume (mL) Cat. No. Quantity (pk)
1
1
1
Purific
atio
n
125
Flash Chromatography System
Flash chromatography, is a rapid form of preparative column chromatography, is especially useful in separation and purification of large scale of
complex organic intermixture, combinatorial chemistry, drug discovery and natural product research and development.
Agela FS-2100 "Chemistry-centric" design for all purpose liquid chromatography applications
Windows interface wizard method development software with touch screen makes purification a snap,we can adjust the distance between the
touch screen and the manager
Compact Profile design for limited fume hood space
Column accepts any compression module/cartridge combination
Pump Patented valveless metering pump, maintenance free, low pulse. Suitable for all organic solvent, good resistance for acid and alkali.
Automatic pressure monitoring & alarm for over/under-pressure conditions
Exposed accessible fluid path for no leaky surprises. Easily maintain all components
Detector adjustable wavelength 190 to 600nm
Fraction High volume fraction collection to correlate fractions with chromatogram display
Split fraction rack design allows pause-free extended runs and off- line tube changes
Peak software generates an optimized purification run. Flow rates are automatically maximized whenever possible to speed purification.
Optimized conditioning with pause for sample injection
Modify the method Pause and resume the run as necessary.
Collect mode Peak, volume, windows
Minimize Method Development Time and Maximize Results!
complete most purification within 20 minutes
Features & Capabilities
Best Value
Guaranteed Product Quality
Innovation to Benefit CustomersOrdering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
-0.1
0.0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1.0
1.1
1.2
0 2 4 6 8 10 12 14 16 18 20
5
10
20
30
40
50
60
70
80
90
105
Time(min)
AgelaAgelaTechnologies
BETTER SOLUTION FOR CHROMATOGRAPHY
Purific
atio
n
126
Specification
Column System
Flow Pump
UV Detector
Fraction Collector
Peak Software
General
Feature/Specification
Purification Channels
Cartridge Sizes
Solid Sample Capacity
Liquid Sample Injection
Solvent Delivery
Flow rate
Gradient (# of solvents)
System Max Pressure
UV Detection
Fraction Tube Sizes
Rack Design
Architecture
Input
Video
Ports
Chromatogram display
Off-line Run Review
Auto Configuration
Sample ID
File system
Power
Dimensions HxWxD (cm)
Warranty
Description
1
4-330g
5mg-33g using prepacked SPE-type barrels or self-loaded cartridges
5-35mL, luer taper syringe port
Patented valveless metering pump
5-100 mL/min. (±1% accuracy), 50-500 mL/min optional
Binary (2) standard (one optional)
100 psi (7bar) included software low / high cut-off alarm
Field changeable filters for 190-600nm wavelength
13, 15, 18, 24mm tube racks, scintillation vials, funnels
Two rack design for extended run without pause and offline tube changes
Touch Screen
Touch Screen
10.4" TFT, solvent resistant
USB,RS-485
Real-time color , with historical pan/zoom/scroll by time & AU
Open saved run information for off-line analysis on the touch screen
"Wizard" guided setup with manual override for advanced users
Sample ID identifies samples in saved run data
Standard Windows with extended file and folder names
100-240V 50/60 Hz
60 x 48 x 48cm( 24" x 19" x 19")
One year limited warranty (ask sales representative for details)
Agela FS-2100 Flash Chromatography Workstation
GC ColumnsDA-1
DA-1MS
DA-5
DA-5MS
DA-35MS
DA-1701
DA-CarBoWax 20M
DA-FFAP
DA-INOWAX
DA-50+MS
DA-1301
DA-624
DA-PLOT Al2O
3 “KCl”
DA-PLOT Al2O
3 “S”
DA-PLOT Al2O
3 “M”
DA-PLOT MoleSieve
DA-PLOT GasPro
DA-PLOT Q
DA-PLOT U
DA-Blood Alcohol Column
128
129
130
131
132
132
133
133
134
135
136
137
138
139
140
141
142
143
144
144
AgelaTechnologies
BETTER SOLUTION FOR CHROMATOGRAPHY
GC
Colu
mns
128
DA-1
100% bonded and crosslinked dimethylpolysiloxane
Non-polar, equivalent to USP phase G2
Solvent rinseable and high temperature limit
Wide range of column dimensions and stationary phase film thicknesses
Low column bleed, inert surface and high efficiency
Most polular for general applications
Similar Phases: DB-1, HP-1, Ultra-1, SPB-1, RTX-1, CP-Si 5CB, BP-1, ZB-1, AT-1, MDN-1, OV-1, OV101
Length(m)
15
15
15
30
30
30
60
60
100
15
15
25
25
25
30
30
30
30
30
30
50
50
50
60
60
60
5
5
5
7.5
10
10
15
15
15
15
30
30
30
30
30
60
Cat.No.
0125-1501
0125-1502
0125-1510
0125-3001
0125-3002
0125-3010
0125-6002
0125-6010
0125-0010
0132-1502
0132-1510
0132-2501
0132-2505
0132-2510
0132-3001
0132-3002
0132-3010
0132-3030
0132-3040
0132-3050
0132-5001
0132-5005
0132-5010
0132-6002
0132-6010
0132-6050
0153-0501
0153-0508
0153-0526
0153-0750
0153-1008
0153-1026
0153-1501
0153-1515
0153-1530
0153-1550
0153-3008
0153-3015
0153-3026
0153-3030
0153-3050
0153-6050
Film(μm)
0.1
0.25
1
0.1
0.25
1
0.25
1
0.5
0.25
1
0.17
0.52
1.05
0.1
0.25
1
3
4
5
0.17
0.52
1.05
0.25
1
5
0.15
0.88
2.65
5
0.88
2.65
0.15
1.5
3
5
0.88
1.5
2.65
3
5
5
Temperature Limit(OC)
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/280
60 to 325/280
60 to 325/280
60 to 325/350
60 to 325/350
60 to 300/320
60 to 325/350
60 to 300/320
60 to 260/280
60 to 325/360
60 to 325/360
60 to 260/280
60 to 260/280
60 to 300/320
60 to 260/280
60 to 320/360
60 to 300/320
60 to 260/280
60 to 260/280
60 to 300/320
60 to 300/320
60 to 260/280
60 to 260/280
60 to 260/280
60 to 260/280
ID(mm)
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.53
0.53
0.53
0.53
0.53
0.53
0.53
0.53
0.53
0.53
0.53
0.53
0.53
0.53
0.53
0.53
Ordering Information
Best Value
Guaranteed Product Quality
Innovation to Benefit Customers
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
GC
Colu
mns
129
DA-1MS
Identical selectivity to DA-1,but with much lower column bleed
Ideal for GC-MS analysis and first choice of new method development
Similar phases: HP 1MS,DB 1MS,BPX-1,AT 1MS,ZB-1,Rtx-1,CP-Sil 5CB
Length(m)
15
30
30
30
30
60
15
25
30
30
30
60
Cat.No.
1125-1502
1125-3001
1125-3002
1125-3005
1125-3010
1125-6002
1132-1502
1132-2505
1132-3001
1132-3002
1132-3010
1132-6002
Film(μm)
0.25
0.1
0.25
0.5
1
0.25
0.25
0.52
0.1
0.25
1
0.25
Temperature Limit(OC)
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
ID(mm)
0.25
0.25
0.25
0.25
0.25
0.25
0.32
0.32
0.32
0.32
0.32
0.32
Ordering Information
AgelaTechnologies
BETTER SOLUTION FOR CHROMATOGRAPHY
GC
Colu
mns
130
DA-5
5% phenyl, 95% methylpolysiloxane, bonded and crosslinked
Non-polar, equivalent to USP phase G27
Solvent rinseable and high temperature limit
Wide range of column dimensions and stationary phase film thicknesses
Low column bleed, inert surface with neutral surface and high efficiency
Most popular for general applications
Similar Phases: DB-5, HP-5, Ultra-5, SPB-5, RTX-5, CP-Sil 8CB, BP-5, ZB-5, AT-5, MDN-5, OV-5, SE-52, SE-54
Length(m)
15
15
30
30
30
60
60
15
15
25
25
25
30
30
30
30
50
50
50
60
60
10
15
15
30
30
30
30
Cat.No.
0525-1502
0525-1510
0525-3001
0525-3002
0525-3010
0525-6002
0525-6010
0532-1502
0532-1510
0532-2501
0532-2505
0532-2510
0532-3001
0532-3002
0532-3005
0532-3010
0532-5001
0532-5005
0532-5010
0532-6002
0532-6010
0553-1026
0553-1515
0553-1550
0553-3008
0553-3015
0553-3026
0553-3050
Film(μm)
0.25
1
0.1
0.25
1
0.25
1
0.25
1
0.17
0.52
1.05
0.1
0.25
0.5
1
0.17
0.52
1.05
0.25
1
2.65
1.5
5
0.88
1.5
2.65
5
Temperature Limit(OC)
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 260/280
60 to 260/280
60 to 300/320
60 to 260/280
60 to 300/320
60 to 300/325
60 to 260/280
60 to 260/280
ID(mm)
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.53
0.53
0.53
0.53
0.53
0.53
0.53
Ordering Information
GC
Colu
mns
131
Identical selectivity to DB-5, but with much lower column bleed
Best surface inertness for wide range of compounds
Ideal for GC-MS analysis or new method development
Similar Phases: HP-5MS, DB-5MS, BPX-5MS, AT-5MS, Rtx-5MS, CP-Sil 8 CB
Length(m)
15
15
15
15
30
30
30
30
60
60
60
60
15
15
15
15
25
30
30
30
30
60
60
60
60
Cat.No.
1525-1501
1525-1502
1525-1505
1525-1510
1525-3001
1525-3002
1525-3005
1525-3010
1525-6001
1525-6002
1525-6005
1525-6010
1532-1501
1532-1502
1532-1505
1532-1510
1532-2505
1532-3001
1532-3002
1532-3005
1532-3010
1532-6001
1532-6002
1532-6005
1532-6010
Film(μm)
0.1
0.25
0.5
1
0.1
0.25
0.5
1
0.1
0.25
0.5
1
0.1
0.25
0.5
1
0.52
0.1
0.25
0.5
1
0.1
0.25
0.5
1
Temperature Limit(OC)
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
60 to 325/350
ID(mm)
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.32
Ordering Information
DA-5MS
Best Value
Guaranteed Product Quality
Innovation to Benefit Customers
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
AgelaTechnologies
BETTER SOLUTION FOR CHROMATOGRAPHY
GC
Colu
mns
132
DA-35MS
Bonded and 35% phenyl backbone 65% dimethylsiloxane phase
Equivalent to USP phase G-42
Inert to many active compounds
Low column bleed to improve detection sensitivity and quantitation
Applications: Acroclors, PCBs, amines, pesticides, pharmaceutical molecules.
Similar Phases: HP-35MS, DB-35, BPX-35, AT-35, Rtx-35, SPB-35
Length(m)
15
30
30
60
15
30
60
15
30
30
Cat.No.
3525-1502
3525-3001
3525-3002
3525-6002
3532-1502
3532-3002
3532-6002
3553-1510
3553-3005
3553-3010
Film(μm)
0.25
0.15
0.25
0.25
0.25
0.25
0.25
1
0.5
1
Temperature Limit(OC)
40 to 320/340
40 to 320/340
40 to 320/340
40 to 320/340
40 to 320/340
40 to 320/340
40 to 320/340
40 to 300/320
40 to 300/320
40 to 300/320
ID(mm)
0.25
0.25
0.25
0.25
0.32
0.32
0.32
0.53
0.53
0.53
Ordering Information
Bonded and crosslingked 14% Cyanopropyl-phenyl 86% dimethylsiloxane phase
Low to mid polarity
Inert to many active compounds
Applications: pesticides, herbiciders, halogenates. Good confirmation of analysis of active compounds on DA 5MS columns.
Similar phases: DB-1701, ZB-1701, BP-10, Rtx-1701, CP-Sil 19 CB
Length(m)
15
15
15
30
30
30
60
60
60
15
Cat.No.
6125-1501
6125-1502
6125-1510
6125-3001
6125-3002
6125-3010
6125-6001
6125-6002
6125-6010
6125-1501
Film(μm)
0.15
0.25
1
0.15
0.25
1
0.15
0.25
1
0.15
Temperature Limit(OC)
20 to 280/300
20 to 280/300
20 to 280/300
20 to 280/300
20 to 280/300
20 to 280/300
20 to 280/300
20 to 280/300
20 to 280/300
20 to 280/300
ID(mm)
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.32
Ordering Information
DA-1701
GC
Colu
mns
133
DA-FFAP
Nitroterephthalic acid modified Polyethylene glycol (PEG)
Bonded and crosslinked
Non-alcohol/aqueous solvent rinseable
High temperature limit
Polar phase equivalent to USP phase G35
Designed for the analysis of volatitle fatty acids and phenols
Simlar Phases: HP-FFAP, DB-FFAP, CB-WAX58 (FFAP) CB, Stabilwax-DA, BP-21, 007-CW, AT-100
Length(m)
15
30
15
25
30
50
10
15
30
Cat.No.
2125-1502
2125-3002
2132-1502
2132-2505
2132-3002
2132-5005
2153-1010
2153-1510
2153-3010
Film(μm)
0.25
0.25
0.25
0.5
0.25
0.5
1
1
1
Temperature Limit(OC)
60 to 240/250
60 to 240/250
60 to 240/250
60 to 240/250
60 to 240/250
60 to 240/250
60 to 240
60 to 240
60 to 240
ID(mm)
0.25
0.25
0.32
0.32
0.32
0.32
0.53
0.53
0.53
Ordering Information
DA-CarBoWax 20M
Polyethylene glycol (PEG), MW 20,000
Non-Bonded non-crosslinked phase
Polar phase, equivalent to USP phase G16
Replacement of packed columns with Carbowax 20M modified
Length(m)
25
50
30
25
50
10
30
Cat.No.
2220-2501
2220-5001
2225-3002
2232-2503
2232-5003
2253-1013
2253-3013
Film(μm)
0.1
0.1
0.25
0.3
0.3
1.33
1.33
Temperature Limit(OC)ID(mm)
0.2
0.2
0.25
0.32
0.32
0.53
0.53
Ordering Information
Best Value
Guaranteed Product Quality
Innovation to Benefit Customers
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
AgelaTechnologies
BETTER SOLUTION FOR CHROMATOGRAPHY
GC
Colu
mns
134
DA-INOWAX
Bonded and crosslinked Polyethylene glycol (PEG)
Solvent washable
Highest temperature limits of bonded PEG phases
Polar phase, equivalent to USP phase G16
Wide applications of polar solvent analysis in food, pharma., cosmetics, petrochemical industries
Similar Phases: HP-INNOWax, DB-WAX, DB-WAXetr, SupelcoWAX 10, CP-WAX 52 CB, CB-WAX, Stablilwax, BP-20, 007-CW, AT-Wax,
Rtx-Wax, ZB-Wax, HP-20M
Length(m)
25
25
50
50
15
15
30
30
30
60
60
60
15
15
30
30
30
60
60
60
15
30
60
Cat.No.
2020-2502
2020-2504
2020-5002
2020-5004
2025-1502
2025-1505
2025-3001
2025-3002
2025-3005
2025-6001
2025-6002
2025-6005
2032-1502
2032-1505
2032-3001
2032-3002
2032-3005
2032-6001
2032-6002
2032-6005
2053-1510
2053-3010
2053-6010
Film(μm)
0.2
0.4
0.2
0.4
0.25
0.5
0.15
0.25
0.5
0.15
0.25
0.5
0.25
0.5
0.15
0.25
0.5
0.15
0.25
0.5
1
1
1
Temperature Limit(OC)ID(mm)
0.2
0.2
0.2
0.2
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.53
0.53
0.53
Ordering Information
GC
Colu
mns
135
Length(m)
12
12
25
25
50
15
15
15
30
30
30
60
15
15
15
30
30
30
60
60
15
30
30
Cat.No.
5020-1201
5020-1203
5020-2501
5020-2503
5020-5003
5025-1501
5025-1502
5025-1505
5025-3001
5025-3002
5025-3005
5025-6002
5032-1501
5032-1502
5032-1505
5032-3001
5032-3002
5032-3005
5032-6002
5032-6005
5053-1510
5053-3005
5053-3010
Film(μm)
0.16
0.31
0.16
0.31
0.31
0.15
0.25
0.5
0.15
0.25
0.5
0.25
0.15
0.25
0.5
0.15
0.25
0.5
0.25
0.5
1
0.5
1
Temperature Limit(OC)ID(mm)
0.2
0.2
0.2
0.2
0.2
0.25
0.25
0.25
0.25
0.25
0.25
0.25
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.32
0.53
0.53
0.53
DA-50+MS
Bonded and crosslinked 50% phenyl backbone 50% dimethylsiloxane phase
Equivalent to USP phase G-3
Inert to many active compounds
Low column bleed to improve detection sensitivity and quantization
Applications: Common drug screens, phenols, PAHs, free steroids, pesticides, herbicides. Good confirmation of the analysis of active
compounds on AB-5MS or AB-35MS columns
Similar Phases: HP-50+, DB -7, DB-17MS, BPX-50, AT-50, Rtx-50, SPB-50
Ordering Information
Best Value
Guaranteed Product Quality
Innovation to Benefit Customers
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
AgelaTechnologies
BETTER SOLUTION FOR CHROMATOGRAPHY
GC
Colu
mns
136
DA-1301
Bonded and crosslinked 6% Cyanopropyl-phenyl 94% dimethylsiloxane phase
Equivalent to USP phase G-43, low to mid polarity
Inert to many active compounds
Applications: pesticides, herbiciders, halogenates. Good confirmation of the analysis of active compounds on DA-5MS or DA-35MS columns
Similar Phases: HP-1301, DB-1301, BP-10, Rtx-1301
Length(m)
15
15
30
30
60
60
15
15
30
30
60
60
15
30
30
Cat.No.
6025-1502
6025-1510
6025-3002
6025-3010
6025-6002
6025-6010
6032-1502
6032-1510
6032-3002
6032-3010
6032-6002
6032-6010
6053-1510
6053-3010
6053-3015
Film(μm)
0.25
1
0.25
1
0.25
1
0.25
1
0.25
1
0.25
1
1
1
1.5
Temperature Limit(OC)ID(mm)
0.25
0.25
0.25
0.25
0.25
0.25
0.32
0.32
0.32
0.32
0.32
0.32
0.53
0.53
0.53
Ordering Information
GC
Colu
mns
137
DA-624
Bonded and crosslinked specially designed stationary phase for EPA methods
Mid-polarity
Inert to many active compounds
Applications: EPA methods: 501.3, 502.2, 503.1, 524.2, 601, 602, 8010, 8015, 8020, 8240,8260
Similar Phases: DB-624, ZB-624, AT-624, Rtx-624, CP-624
Length(m)
25
30
60
30
60
30
60
75
Cat.No.
6220-2511
6225-3014
6225-6014
6232-3018
6232-6018
6253-3030
6253-6030
6253-7530
Film(μm)
1.12
1.4
1.4
1.8
1.8
3
3
3
Temperature Limit(OC)ID(mm)
0.2
0.25
0.25
0.32
0.32
0.53
0.53
0.53
Ordering Information
Best Value
Guaranteed Product Quality
Innovation to Benefit Customers
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
AgelaTechnologies
BETTER SOLUTION FOR CHROMATOGRAPHY
GC
Colu
mns
138
DA-PLOT Al2O
3 “KCl”
Least selective ("polar") aluminum oxide phase
Deactivated with KCl
Good baseline separation of most C1 to C10 hydrocarbons
Olefins may co-elute with corresponding paraffin over C5
Minimum surface adsorption of dienes and halocarbons
ASTM recommended phase
Preferred choice of hydrocarbon separation
Similar Phases: HP-PLOT Al2O3/KCL, GS-Alumina/KCL, CP-Al2O3/KCl PLOT, ZB-Alumina/KCl
Length(m)
25
30
15
30
50
15
30
50
60
Cat.No.
8125-2505
8125-3005
8132-1508
8132-3008
8132-5008
8153-1515
8153-3015
8153-5015
8153-6015
Film(μm)
5
5
8
8
8
15
15
15
15
Temperature Limit(OC)ID(mm)
0.25
0.25
0.32
0.32
0.32
0.53
0.53
0.53
0.53
Ordering Information
GC
Colu
mns
139
Length(m)
25
30
15
30
50
15
30
50
60
25
30
15
30
50
15
30
50
60
Cat.No.
8225-2505
8225-3005
8232-1508
8232-3008
8232-5008
8253-1515
8253-3015
8253-5015
8253-6015
8325-2505
8325-3005
8332-1508
8332-3008
8332-5008
8353-1515
8353-3015
8353-5015
8353-6015
Film(μm)
5
5
8
8
8
15
15
15
15
5
5
8
8
8
15
15
15
15
Temperature Limit(OC)ID(mm)
0.25
0.25
0.32
0.32
0.32
0.53
0.53
0.53
0.53
0.25
0.25
0.32
0.32
0.32
0.53
0.53
0.53
0.53
Ordering Information
DA-PLOT Al2O3 “S”
Good selective (mid "polar") aluminum oxide phase
Deactivated with Na2SO4 salt
Excellent baseline separation of C1 to C10 hydrocarbons, excellent of C3, C4 isomer baseline separations
Less accurate quantitations of dienes and halocarbons
ASTM recommended phase
Preferred choice of hydrocarbon separation
Similar Phases: HP-PLOT Al2O3/"S", GS-Alumina, CP-Al2O3/Na2SO4 PLOT, ZB-Alumina, RT-Alumina
Best Value
Guaranteed Product Quality
Innovation to Benefit Customers
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
AgelaTechnologies
BETTER SOLUTION FOR CHROMATOGRAPHY
GC
Colu
mns
140
DA-PLOT Al2O3 “M”
Strong selective (most "polar") aluminum oxide phase
Deactivated with Na2MoO4 salt
Mostly baseline separation of C1 to C10 hydrocarbons, good baseline separation of cyclopropane from propylene
Accurate quantitations of dienes and halocarbons
ASTM recommended phase
Preferred choice of hydrocarbon separation
Similar Phases: HP-PLOT Al2O3/"M", GS-Alumina, ZB-Alumina
Length(m)
25
30
15
30
50
15
30
50
60
Cat.No.
8325-2505
8325-3005
8332-1508
8332-3008
8332-5008
8353-1515
8353-3015
8353-5015
8353-6015
Film(μm)
5
5
8
8
8
15
15
15
15
Temperature Limit(OC)ID(mm)
0.25
0.25
0.32
0.32
0.32
0.53
0.53
0.53
0.53
Ordering Information
GC
Colu
mns
141
Length(m)
5
15
30
15
30
5
15
30
15
30
Cat.No.
8432-0512
8432-1512
8432-3012
8432-1525
8432-3025
8453-0525
8453-1525
8453-3025
8453-1550
8453-3050
Film(μm)
12
12
12
25
25
25
25
25
50
50
Temperature Limit(OC)ID(mm)
0.32
0.32
0.32
0.32
0.32
0.53
0.53
0.53
0.53
0.53
Ordering Information
DA-PLOT MoleSieve
Molecular Sieve, Zeolite, 5A
Thin and thick coatings for push limit separations, e.g. Ar/O2 separation at 35C, O2/N2 with 15sec
Truly immobilized coating and smooth baseline workable for valve switching
Preferred column to analyze fixed gases from hydrocarbon streams
Elution order, H2, He, Ne, O2, N2, CH4, CO - Regeneration of performance from water/CO2 adsorptions at 250 for 3hr
ASTM recommended phase
Noble gas, air from methane, fuel cell, etc
Often used with other PLOT columns for fixed gas separation from sample matrix
Similar Phases: HP-PLOT Molesieve, CP-PLOT MoleSieve.
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
Best Value
Guaranteed Product Quality
Innovation to Benefit Customers
AgelaTechnologies
BETTER SOLUTION FOR CHROMATOGRAPHY
GC
Colu
mns
142
DA-PLOT GasPro
Proprietary Silica base phase
Very inert to wide range of gases including sulfur gases and Freons, symmetric peak shapes
Truly immobilized coating and smooth baseline workable for valve switching
Preferred column to analyze sulfur gases from hydrocarbon streams and air
Separation of CO and CO2
No effect of water on retention time
Regeneration of performance from water adsorptions at 250 for 3hr
ASTM recommended phase
GC-MS applicable
Replacement: GS-GasPro, CP-Silica PLOT
Length(m)
5
15
30
Cat.No.
8532-0505
8532-1505
8532-3005
Film(μm)
5
5
5
Temperature Limit(OC)ID(mm)
0.32
0.32
0.32
Ordering Information
GC
Colu
mns
143
Length(m)
15
30
15
30
Cat.No.
8632-1515
8632-3015
8653-1530
8653-3030
Film(μm)
15
15
30
30
Temperature Limit(OC)ID(mm)
0.32
0.32
0.53
0.53
Ordering Information
DA-PLOT Q
Divinylbenzene polymer particle, non-polar, close to traditional PoraPak Q
Truly immobilized coating and smooth baseline workable for valve switching and liquid sample introduction at low temperatures
Highest temperature limit, up to 280/300
Baseline separation of Air, CO2, water, sulfur gases, hydrogen chlorine, ammonia/light amines from hydrocarbons such as refinery
stream, natural gas, and engine exhaust
Excellent separation of C1 to C3 isomers, good separations of C3 to C12, halocarbons
Excellent inertness and long lifetime of water/alcohol sample, a good secondary choice of wax column for alcohol analysis
Excellent tolerance to strong acid/base sample without affecting retention time
Good anti-oxidation at 280 and fast regeneration of performance
Observed slight sulfur gas, hydrogen chlorine, and amine absorption
Water peak is tailing, but no effect on retention
Good choice for analyzing low level solvent residues from wide samples, such as headspace, aqueous, or organic solutions
Preferred column for residue analysis in petroleum industry, pharmaceutical, environmental, chemical weapon detection
Preferred column to analyze polar compounds including oxygenates from hydrocarbon streams: natural gas, refinery gas, dienes,
ethylene, propylene
Replacement: HP-PLOT Q, GS-Q, CP-PoraPLOT Q HT, Supel PLOT Q, Rt-Q, ZB-PLOT Q
Best Value
Guaranteed Product Quality
Innovation to Benefit Customers
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
AgelaTechnologies
BETTER SOLUTION FOR CHROMATOGRAPHY
GC
Colu
mns
144
DA-PLOT U
Divinylbenzene and Ethylene glycol dimethacrylate copolymer particle, polar, close to traditional PoraPack U
Truly immobilized coating and smooth baseline workable for valve switching and liquid sample introduction at low temperatures
Improved separation and selectivity of Air, CO2, water, sulfur gases, hydrogen chlorine, ammonia/light amines from hydrocarbons
Symmetric water peak shape, water elute after C3
Excellent separation of C1 to C3 isomers, good separations of C3 to C12, halocarbons
Excellent inertness and long lifetime of water/alcohol sample, a good secondary choice of wax column for alcohol analysis
Excellent tolerance to strong acid/base sample without affecting retention time
Observed slight sulfur gas, hydrogen chlorine, and amine absorption
Good choice for analyzing low level solvent residues from wide samples, such as headspace, aqueous, or organic solutions
Preferred column for residue analysis in petroleum industry, pharmaceutical, environmental, chemical weapon detection
Preferred column to analyze polar compounds including oxygenates from hydrocarbon streams: natural gas, refinery gas, dienes,
ethylene, propylene
Replacement: HP-PLOT U, CP-PoraPLOT U
Length(m)
7.5
Cat.No.
8932-0710
Film(μm)
10
Temperature Limit(OC)ID(mm)
0.32
Ordering Information
DA-Blood Alcohol Column
Proprietary PLOT phase
Reliable blood alcohol analysis
Tolerance of direct sample injection
Excellent confirmation column to other blood alcohol columns using t-butanol as internal standard
Length(m)
15
30
30
15
30
Cat.No.
8732-1510
8732-3010
8732-3020
8753-1520
8753-3020
Film(μm)
10
10
20
20
20
Temperature Limit(OC)ID(mm)
0.32
0.32
0.32
0.53
0.53
Ordering Information
HPLC Accessories and Supplies
Column Savor –Online Filter
HPLC Mixer
HPLC Fittings and Tubing
HPLC Injector and Sample Loop
Automated Switching Valve
Automatic Column Selector
HPLC Column Heater
UV Lamps
SPE Accessories and Supplies
SPE Vacuum Manifolds
Multipurpose SPE Work-station
SPE-1 Automated Processing Station
Positive Pressure SPE Work-station
Robotic SPE Work-station
Solvent Evaporator
Empty Column and Accessories
Pipettes and Accessories
GC Accessories and Supplies
Septa
Injection Port Liners
Ferrules
Column Scribe
1/32” Ultra Low Mass External Unions
Filtration Products
Syringe Filters
96 well filtration plates and collection plates
Membrane Filters
Glass Filtration System
Chemical Resistance Guide for Membranes
Vials and Caps
Screw Neck Vials and Caps
Crimp Neck Vials and Caps
Plastic Vials (Micro-vials for LC-MS Autosamplers)
146
146
146
147
149
149
150
150
151
153
153
153
154
154
154
154
155
156
157
157
157
158
161
161
162
162
164
164
164
165
166
166
166
166
Equipments, Accessories and
SuppliesAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories andAccessories and
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Equipments, Accessories and
Equipments, Accessories and
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Supplies
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Supplies
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Supplies
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Supplies
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Supplies
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Supplies
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Supplies
Equipments, Accessories and
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Supplies
Equipments, Accessories and
Supplies
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Supplies
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Equipments, Accessories and
Supplies
Equipments, Accessories and
Supplies
AgelaTechnologies
BETTER SOLUTION FOR CHROMATOGRAPHY
Accessories/S
upplie
s
146
Column Savor –Online Filter
HPLC Accessories and Supplies
Online Filters
Description
PEEK, ZDV
Cat.No.
OFP-001
Ordering Information
Bottom-of-the-Bottle Solvent Filters
10um Filter Assembly¹,for 1/8˝ OD tubing with A•520 replaceable filter cup
2um Filter Assembly¹,for 1/8˝ OD tubing with A•522 replaceable filter cup
10um Filter Assembly²,for 3/16˝ OD tubing with A•520 replaceable filter cup
2um Filter Assembly²,for 3/16˝ OD tubing with A•522 replaceable filter cup
Replacement Filter Cups (For AA•550-AA•553 Filters)
10um Stainless Steel Cups,10-pk
2um Stainless Steel Cups,10-pk
Inlet Solvent Filters
Totally PEEK polymer wetted surfaces
Biocompatible and inert
For analytical systems
High-pressure applications
HPLC Mixer
Static Bed Mixers
Mixer cartridge Vol. Applicable Flowrate(µL) Cat.No.
50
150
250
20-150
150-500
500
SMX050
SMX150
SMX250
Ordering Information
Cat.No.
AA•550
AA•551
AA•552
AA•553
AA•520x
AA•522x
Accessories/S
upplie
s
147
Cat.No. Tubing Size /Nut Threads QtyMaterial
Kel-F
PEEK
PEEK
PEEK
PEEK/Kel F
Kel F
AF 100
AF 120
AF 127
AF 130
AM 645
AP 100
1/16”, 10 • 32
1/16”, 10 • 32
1/16”, 10 • 32
1/16”, 10 • 32
1/32”, 6 • 40
1/8”, 1/4 • 28
10-pk
10-pk
10-pk
10-pk
10-pk
each
Cat.No. Tubing Size /Nut Threads QtyMaterial
Delrin
PEEK
Long PEEK
Short PEEK
Delrin
PEEK
Long PEEK
Short PEEK
AF 200
AF 300
AF 330
AF 331
AF 200n
AF 300n
AF 330n
AF 331n
1/16”OD, 10 • 32
1/16”OD, 10 • 32
1/16”OD, 10 • 32
1/16”OD, 10 • 32
1/32”OD, 10 • 32
1/32”OD, 10 • 32
1/32”OD, 10 • 32
1/32”OD, 10 • 32
10-pk
10-pk
10-pk
10-pk
ea
ea
ea
ea
Two-piece Fingertight Fittings
AF-120 AF-130 AF-127
AF-300 AF-330 AF-331 AF-200
One-piece Fingertight Fittings
Unions
Material
PEEK ZDV
Stainless Steel ZDV
Material
PEEK
Stainless Steel
Type
High Pressure, webbed
High Pressure, webbed
Type
High Pressure
High Pressure
OD
1/16"
1/16"
OD
1/16"
1/16"
Cat.No.
Cat.No.
TEP-001
TES-001
Tees
Connections (Unions and Tees)
HPLC Fittings and Tubing
All of One-piece Fingertight Fittings are designed to be used with 1/16” OD tubing, except the AM-645(1/32”) and AP-100(1/8”)
UNP-001
UNS-001
Best Value
Guaranteed Product Quality
Innovation to Benefit Customers
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
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BETTER SOLUTION FOR CHROMATOGRAPHY
Accessories/S
upplie
s
148
PEEK Tubing
PEEK polymer tubing is a good replacement of stainless steel tubing used commonly in HPLC systems. It has many advantages over the
stainless steel tubing, which includes better chemical inertness, better biocompatibility, and convenience to cut. Additionally, PEEK tubing has
a very smooth internal surface, which cause less turbulence than metal tubing. Unlike other polymer tubing, PEEK tubing can tolerate high
pressure (up to 7000 psi) and high temperature (up to 100 oC).
1/16” OD x 5’ L
1/8” OD x 5’ L
1.8 mm OD x 5’ L
1/32” OD x 5’ L
Cat.No.
AT1560
AT1561
AT1535
AT1562
AT1536
AT1531
AT1565
AT1532
AT1533
AT1538
AT1537
AT1534
AT1544
AT1545
AT1546
AT1547
AT1540
AT1539
AT1579
AT1578
AT1576
AT1577
AT1575
AT1580
AT1581
AT1568
AT1569
I D
0.0025˝( 65 μm )
0.004˝ (100 μm )
0.005˝ (125 μm )
0.006˝ (150 μm )
0.007˝ (175 μm )
0.010˝ (0.25mm)
0.015˝ (0.40mm)
0.020˝ (0.50mm)
0.030˝ (0.75mm)
0.040˝ (1.00mm)
0.055˝ (1.40mm)
0.062˝ (1.60mm)
0.080˝ (2.00mm)
0.010˝ (0.25mm)
0.020˝ (0.50mm)
0.030˝ (0.75mm)
0.042˝ (1.05mm)
0.055˝ (1.40mm)
0.025˝ ( 65μm )
0.035˝ ( 90μm )
0.050˝ ( 125μm )
0.070˝ ( 175μm )
0.080˝ ( 200μm )
0.090˝ ( 230μm )
0.100˝ (0.25mm)
0.150˝ (0.40mm)
0.200˝ (0.50mm)
Pressure Rating
7,000psi (483bar)*
7,000psi (483bar)*
7,000psi (483bar)*
7,000psi (483bar)*
7,000psi (483bar)*
7,000psi (483bar)*
7,000psi (483bar)*
7,000psi (483bar)*
7,000psi (483bar)*
5,000psi (345bar)*
500psi ( 34bar)*
4,000psi ( 276bar)*
3,000psi ( 207bar)*
7,000psi (483bar)*
7,000psi (483bar)*
7,000psi (483bar)*
5,000psi (345bar)*
500psi ( 34bar)*
5,000psi (345bar)*
5,000psi (345bar)*
5,000psi (345bar)*
5,000psi (345bar)*
5,000psi (345bar)*
5,000psi (345bar)*
5,000psi (345bar)*
5,000psi (345bar)*
5,000psi (345bar)*
Tubing Holders
Tubing Holders For Polymer Tubing
AP•740
AP•741
AP•815
90°Tubing Holder for 1/16˝ OD tubing, polystyrene
180°Tubing Holder for 1/16˝ OD tubing, polystyrene
90°Tubing Holder for 1/8˝ - 5/16˝ OD tubing, PVT
Cat.No.
Accessories/S
upplie
s
149
Tubing Cutters
Tubing Cutters For Polymer Tubing
AA•327
AA•329
AA•350
AA•328
AF•262x
AF•264x
AF•267Bx
AFS•315
AFS•315•02
Standard Polymer Tubing Cutter* for 1/16˝ and 1/8˝ OD tubing
Large Bore Polymer Tubing Cutter* for 3/16˝ - 5/16˝ OD tubing
Capillary Polymer Tubing Cutter* for 360μm- 1/32˝ OD tubing Includes(1)F•262x10-pack of sleeves
Replacement Blades for A •327, A •329and A •350 5-pk
Replacement Sleeves for A •350,0.0155˝ID,Green, for cutting 360μm OD tubing 10-pk
Alternate Sleeves for A •350,0.021˝ID,Natural, for cutting 510μm OD tubing 10-pk
Alternate Sleeves for A •350,0.033˝ID,Blue, for cutting 1/32˝ OD tubing 10-pk
Precision Fused Silica Tubing Cutter
Maintenance Kit for FS•315
HPLC Injector and Sample Loop
Sample Injector
Type Description Cat.No.
Rheodyne 7725 Injector
Rheodyne 9725 Injector
Rheodyne 3725 Injector
Standard Analytical Sample Injector; Stainless steel
Standard Analytical Sample Injector; Peek
Preparative Sample Injector
INJ7725
INJ9725
INJ3725
Sample Loop
Type Cat.No.
5µL Stainless Steel
5µL Peek
20µL Stainless Steel
20µL Peek
50µL Stainless Steel
50µL Peek
100µL Stainless Steel
100µL Peek
1mL Stainless Steel
1mL Peek
5mL Stainless Steel
10mL Stainless Steel
LPS0005SS
LPS0005PK
LPS0020SS
LPS0020PK
LPS0050SS
LPS0050PK
LPS0100SS
LPS0100PK
LPS1000SS
LPS1000PK
LPS5000SS
LPS0000SS
Automated Switching Valve
Used for 2D-LC or on-line SPE/LC
Editable valve diagram.
Flexible in choosing valve number and valve type.
The valve types can be 2-position/6-port; 2-position/8-port; 2-position/10- port; 6-position
or 10 position.
One module can be installed with 1 or 2 valves. Two modules can be used together to
make a 4-valve platform or be sued as two independent modules.
Ordering Information
Type Cat.No.
LC-04SP Valve System ASV0002-0
Cat.No.
Best Value
Guaranteed Product Quality
Innovation to Benefit Customers
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
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HPLC Column Heater
Cat.No.
Dimensions (H×W×D)
Operating Temperature
Temperature Accuracy
Compatible Column Quantity and Length
Power
Weight
CC-100
510mm×110mm×65mm
Above 5 100
±0.1
2×300mm
220V/50Hz/1A
4Kg
Technical specification
Automatic Column Selector
Connect up to 6 HPLC columns
Controllable by both panel keys and computer
Easy key operation for setting method and sequence
Compact design and easy access to columns
Compatible with any HPLC
Compatible with other LC-04SP valves
Cat.No.: AST-006
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UV LampsAgela Technologies offers UV lamps of the same or better quality as a low cost replacement for many of those manufactured by original HPLC
manufacturers, such as Waters, Agilent, Varian, Shimazu and Perkin-Elmer.
Original Instrument Part Number Agela Lamps Part Number Type of Lamps
AL 610
ALH 10
AL 890
AL 610
AL 295
ALH 10
ALH l0
ALH l6
ALH 855
ALH 11
ALH 12
ALH 15
ALH 13X2
AL 850
ALH 10
ALH 14
ALH 13
ALH 13X2
AL 850
ALH l4
ALH 10
W
D2
P/Xe
W
D2
D2
D2
D2
W
D2
D2
D2
D2
mf
D2
D2
D2
D2
W
P/Xe
W
D2
D2
l037
l040A,1040M
1046
l047A
79853A
79853C,79854A
79875A
79880A,7988lA
8450A
8450A
8451A
8452A
8453A,8453E
8453A
8453A
G1306A
G13l4A
Gl315A&B,Gl365A&B
Gl315A&B,Gl365A&B
Gl3l5A&B,Gl365A&B
Gl32l A
Gl362
HP3D/CE
Series I&II
Agilent
Original Instrument Part Number Agela Lamps Part Number Type of Lamps
AL 305
ALH 80
AL 830
ALH 81
AL 935
AL 830
ALH 80
AL 835
D2
D2
W
D2
W
W
D2
W
SPD-7A, 7AV
SPD-10A, 10 AV, 10AVP
SPD-10 AV, 10AVP
SPD-M1A
SPD-M6A, M10A, M10AV
SPD-M6A, M10A
SPD-M10AV
SPD-M10AVP
SPD-M10AVP
Shimadzu
Best Value
Guaranteed Product Quality
Innovation to Benefit Customers
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
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Original Instrument Part Number Agela Lamps Part Number Type of Lamps
AL 230
AL 230
AL 230
AL 230
AL 230
ALH 910
ALH 80
ALH 80
ALH 950
AL 305
ALH 950
ALH 53
ALH 40
ALH 910
AL 800
AL 230
AL 305
ALH 950
AL 305
AL 305
D2
D2
D2
D2
D2
P/Xe
P/Xe
D2
D2
W
P/Xe
D2
P/Xe
D2
D2
P/Xc
Xe
P/Xe
D2
D2
P/Xe
D2
D2
AA200,275,300
AA400,600,640
AA775,800,875
AA975,l275,l475
AA3000
DMS90
Eclipse
FL-l,2
LC-5000,5500
PDA,1
PDA-2
PDA-2
Pro Star
pro Star 310
Pro Star 320
Pro Star 330
Pro Star 340,345
Pro Star 360
Pro Star 363
Pro Star 364
Spectra A10,20,30,40
Star 9050
1JV•1,lA
UV50,100,200
VIsta 5500
Varian
Original Instrument Part Number Agela Lamps Part Number Type of Lamps
AL 770
AL 820
AL 720
AL 7l5
ALHA l
ALH l
AL 800
AL 205
ALH 3O
ALH 3l
ALHA 34
ALH 34
ALH 33
ALH 925
ALH 36X2
ALH 33
ALH 3l
Hg
Hg
Cd
Zn
D2 Lamp Ass’y
D2 Replacement
Xe
D2
D2
D2
D2 Lamp Ass’y
D2 Replacement
D2
Xe
D2
D2
D2
40l,403,404
420
440,44l
441
441
450
450
470,474
480,48l
484
486
990,99l,994
990,991,994
996
2475
2487,2488
2996
LC Module l/3455l System
Waters
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SPE Vacuum Manifolds
SPE Accessories and Supplies
Type
12 port Model
24 port Model
96well plate Model
Cat.No.
VM0012
VM0024
VM0096
Ordering Information
Multipurpose SPE Work-station
SPE Vaccum Manifold for SPE sample preparation, filtration and elution are available in 12
and 24 port configurations. These manifolds permit consistent extraction and filtration results.
Multiple sample processing with these manifolds consist of a clear glass chamber and lid, to
which a vacuum is applied to draw solvents and sample through an SPE column, cartridge,
or disk. The lid is CNC machined, solvent resistant, low extractable virgin polypropylene. The
lid is autoclavable, and will not wrap. The female Lure inlets and male Lure outlets are
molded of pure polypropylene. Adjustable racks placed in the glass chamber will accommo-
date a variety of sample collection vessels, including test tubes, autosampler vials, volumet-
ric flasks, and Erlenmeyer flasks. Eluants are deposited directly into the collection vessel of
choice via polypropylene, or optional stailness steel or Teflon needles.
Ease for operation: easier to change the collection tubes
Flexibility: can be used for vacuum or positive pressure mode;
more choices for tube size
Multifunction: SPE processing and solvent evaporation
Solid: made of aluminum alloy instead of fragile glass
Compatible with automated liquid handling systems
Cat.No.: MST-001
SPE-1 Automated Processing Station
Designed for sample preparation in trace analysis of food and environmental samples.
Process up to 6 samples per batch without attendance.
Up to 5 solvents can be used for column conditioning and multi step elution.
Two fractions can be collected for each sample.
Accommodate SPE cartridges and traditional cleanup columns.
Exhaustive sample injection.
Easy operation with only 7 buttons.
Small footprint and computer-free operation
Cat.No.: APT-001
This is specially designed to replace conventional SPE manifolds
and solvent evaporators by an integrated and versatile workstation.
It features:
Best Value
Guaranteed Product Quality
Innovation to Benefit Customers
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
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Cat.No.
APSP-48
APSP-22
APSP-23
APSP-24
APSP-19
APSP-20
APSP-21
APSP-25
APSP-26
APSP-27
APSP-28
Description
Positive Pressure Processor-48 Position
Rack for 1 ml SPE Columns
Rack for 3 ml SPE Columns
Rack for 6 ml SPE Columns
Collection Tube Rack, 12 x 75 mm Tubes
Collection Tube Rack, 13 x 100 mm Tubes
Collection Tube Rack, 16 x 100 mm Tubes
Collection Vial Rack, 12 x 32 mm Auto-Sampler
Waste Bin
48-Column Sealing Gasket
Gas Supply Adapter
Qty/Pk
1
1
1
1
1
1
1
1
1
1
1
Ordering Information
Positive Pressure SPE Work-station
48 position
96 position
Robotic SPE Work-station
Cat.No.: RST-0096
24 well to 96 well reformatting
96 and 384 well dispensing
Automated Tip changing
96 well and 384 well liquid level sensing
12 decks for large sample processing
Product Features
Solvent Evaporator
Combination of heating and vacuum for speedy evaporation of a variety of solvents at relative lower temperature (safe, faster and better for
unstable compounds)
Simple operation, no programming set-up needed
Adjustable gas pressure and flow
Multi-size block interchangeable, accepts tubes OD from 11 to 29mm
Optional automatic shutoff, for safe and fast parallel enrichment of various samples
Small footprint, only 18(W)x16(D)inch bench area
Broad customization configurations
Economic and reliable
1.Height post
2.Vacuum vent
3.Needle piercing gas manifold
4.Sample tubes
5.Temperature controller
6.System flow controller
Multi-Needle manifold with individually
controlled flow
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Dimension
Weight
Power
Mount
Working environment
Well plate dimension
Number of Well Plate
Tube OD
Tube length
Temperature
Temperature accuracy
Gas pressure
Gas flow
Needle
Gas/vacuum connection fitting
Auxiliary vacuum
18(W)x20(H)x16(D) inch
45(W)x50(H)x40(D)cm
20lb/10kg
90-120VAC, 50-60Hz, 500VA
Bench stand
4 to 40°C, Humidity <80%
5(W)x4.5(D)x2(H)inch127(W)x110(D)x50(H)mm
12, 15, 30, 48
11-29mm
125-200(mm)
RT-150°C
+-1°C
30psi (2bar) max
0-5liter/min
Stainless steel, 1.6(OD)x150(L)mm
Hose barb 1/8 flow port
5-12psi, 200-700mmHg
Technical Specifications:
Empty Columns and Accessories
Description
1mL, 100/pk
3mL, 100/pk
6mL, 100/pk
1mL, 3mL, 6mL column,12/pk
Tube Part No.
050901
050903
050906
050909
Frit Part No.
050911
050913
050916
Ordering Information
Description
Solvent evaporator, including a multi-needle manifold, inert gas flow module,
heater & controller, 120VAC, 1year limited warranty.
Order multi-well blocks and manifold plates separately!
Solvent evaporator, including individual flow controlled needle manifold,
heater & controller, 120VAC, 1year limited warranty
Order multi-well blocks and manifold plates separately!
Solvent evaporator, 48 or 96well plate format, including purge manifold,
inert gas heating & controller, 120VAC, 1year limited warranty
Cat.No.
GD-101(regular system)
GD-102-xx, xx is number of wells of block
GD-103-48
GD-103-96
Ordering Information
Best Value
Guaranteed Product Quality
Innovation to Benefit Customers
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
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Pipettes and Accessories
Cat No.
A722001
A722004
A722010
A722015
A722020
A722025
A722030
A722035
A722040
A722045
A722050
A722055
Volume (μL)
5
10
20
25
50
100
200
250
500
1000
2000
5000
Mechanical Pipette (Fixed Volume)
Cat No.
AD781348
AD781349
AD780006
AD780007
AD780019
AD780020
AD780046
Qty
FT10μL
FT10μL
FT300μL
FT300μL
FT1000μL
FT1000μL
FT1200μL
Pipette Tips
Cat No.
8 - Channel Pipette
A720210
A720220
A720240
Volume (μL)
0.5-10
5-50
50-300
Increment (μL)
0.1
0.5
5
Cat No.
12 - Channel Pipette
A720310
A720320
A720340
Volume (μL)
0.5-10
5-50
50-300
Increment (μL)
0.1
0.5
5
Cat No.
Single Channel Pipette
A720005
A720000
A720020
A720080
A720050
A720070
A720030
A720060
A720040
A720110
Volume (μL)
0.1-2.5
0.5-10
5-50
2-20
10-100
20-200
50-200
100-1000
200-1000
1000-5000
Increment (μL)
0.05
0.1
0.5
0.5
1
1
1
5
5
50
Mechanical Pipette (AdjustableVolume)
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Septa
GC Accessories and Supplies
Septa BTO
Cat.No.
B02-1002
B02-1040
B02-1031
B02-1001
B02-1032
Description
Septa BTO 5mm Center guide (50/pk)
Septa BTO 3/8" (9.5mm) (50/pk)
Septa BTO 11.5mm Center guide (50/pk)
Septa BTO 11mm (7/16") Center guide (50/pk)
septa BOT 17mm,Center guide
Maximun temperature 400
Virtually eliminates injection-port sticking
Preconditioned packaged in glass to prevent contamination
Advanced Green 3
Cat.No.
B02-1006
B02-1005
B02-1041
Description
Septa AG3 5mm Center guide (50/pk)
Septa AG3 11mm (7/16") Center guide (50/pk)
Septa AG3 3/8" (9.5mm) (50/pk)
True low-bleed, high temperature green septum.
Each batch GC-FID tested.
Ideal for use with low-bleed, “Mass Sepc” capillary columns
For Agilent Capillary GC Instruments
Cat.No.
B02-3004
B02-3005
B02-3007
B02-3008
B02-3009
B02-3011
B02-3012
B02-3013
B02-3015
B02-3019
B02-3020
B02-3022
B02-3030
B02-3031
B02-3032
B02-3033
B02-3034
B02-3036
Description
Liner, 4 mm,79mm length, Single Taper w/ Sil Wool
Liner, 4 mm,79mm length, Single Taper w/ Sil Wool
Liner, 4 mm,79mm length, Single Taper w/ Sil Wool
Liner, 4 mm,79mm length, Double Taper
Liner, 4 mm,79mm length, Double Taper
Liner, 4 mm,79mm length, Double Taper
Liner, 4 mm,79mm length, Single Taper
Liner, 4 mm,79mm length, Single Taper
Liner, 4 mm,79mm length, Single Taper
Liner, 4mm,79mm length, with Silanized Wool
Liner, 4mm,79mm length, with Silanized Wool
Liner, 4mm,79mm length, with Silanized Wool
Liner, 2 mm,79mm length, Splitless, Glass
Liner, 2 mm,79mm length, Splitless, Glass
Liner, 2 mm,79mm length, Splitless, Glass
Liner, 2 mm,79mm length Splitless, Quartz
Liner, 2 mm,79mm length Splitless, Quartz
Liner, 2 mm,79mm length Splitless, Quartz
Qty.
1/pk
5/pk
10/pk
1/pk
5/pk
10/pk
1/pk
5/pk
10/pk
1/pk
5/pk
10/pk
1/pk
5/pk
10/pk
1/pk
5/pk
10/pk
Injection Port Liners
Organosilane deactivated borosilicate glass (quartz where notes). Guaranteed inertness for phenols, organic acids and bases. Glass wool is
also deactivated. These capillary injection port liners provide a clean, non-adsorptive surface for your samples. Regular replacement of the liner
protects your capillary columns from potentially harmful non-volatiles that might otherwise deposit on your columns.
Maximum temperature 400
Extended temperature range, low-bleed
Best Value
Guaranteed Product Quality
Innovation to Benefit Customers
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
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For Perkin-Elmer Capillary GCs
Cat.No.
B02-3201
B02-3202
B02-3203
B02-3204
B02-3205
B02-3206
B02-3207
B02-3208
B02-3209
B02-3210
B02-3211
B02-3212
Description
Liner, 4 mm Split, 92mm length ,Autosystem for PerkinElmer GC
Liner, 4 mm Split, 92mm length ,Autosystem for PerkinElmer GC
Liner, 4 mm Split, 92mm length ,Autosystem for PerkinElmer GC
Liner, 4 mm Split,92mm length Autosystem w/ Wool for PerkinElmer (ea)
Liner, 4 mm Split,92mm length Autosystem w/ Wool for PerkinElmer (ea)
Liner, 4 mm Split,92mm length Autosystem w/ Wool for PerkinElmer (ea)
Liner,2mm Splitless,92mm length, Autosystem for PerkinElmer GC
Liner,2mm Splitless,92mm length, Autosystem for PerkinElmer GC
Liner,2mm Splitless,92mm length, Autosystem for PerkinElmer GC
Liner,100mm, Dimple Spliter for PerkinElmer GC
SLiner,100mm, Dimple pliter for PerkinElmer GC
Liner,100mm, Dimple Spliter for PerkinElmer GC
Qty.
1/pk
5/pk
10/pk
1/pk
5/pk
10/pk
1/pk
5/pk
10/pk
1/pk
5/pk
10/pk
For Varian Capillary GCs
Cat.No.
B02-3103
B02-3104
B02-3105
B02-3112
B02-3113
B02-3114
B02-3115
B02-3116
B02-3117
B02-3118
B02-3119
B02-3120
Description
4 mm Open Split w/ Sil. Wool for Varian 1075/1077
4 mm Open Split w/ Sil. Wool for Varian 1075/1077
4 mm Open Split w/ Sil. Wool for Varian 1075/1077
Liner, Frit Split for Varian 1075/1077
Liner, Frit Split for Varian 1075/1077
Liner, Frit Split for Varian 1075/1077
Liner, 4 mm Open Split, for Varian 1075/1077
Liner, 4 mm Open Split, for Varian 1075/1077
Liner, 4 mm Open Split, for Varian 1075/1077
Liner, 74mm length, Splitless, Glass for Varian 1075/1077 (ea)
Liner, 74mm length, Splitless, Glass for Varian 1075/1077 (ea)
Liner, 74mm length, Splitless, Glass for Varian 1075/1077 (ea)
Qty.
1/pk
5/pk
10/pk
1/pk
5/pk
10/pk
1/PK
5/PK
10/PK
1/pk
5/pk
10/pk
Vespel Ferrules-Sold in Packages of 10
Cat.No.
1/16" ferrules
1/8" ferrules
1/4" ferrules
1/16" to 0.3mm ferrules
1/16" to 0.4mm ferrules
1/16" to 0.5mm ferrules
1/16" to 0.8mm ferrules
1/16" to 1.0mm ferrules
1/16" to 1.2mm ferrules
1/16" to 0.4mm -Agilent style
1/16" to 0.5mm -Agilent style
1/16" to 0.8mm -Agilent style
1/8" to 0.4mm Vespel
1/8" to 0.5mm Vespel
1/8" to 0.8mm Vespel
1/8" to 1/16" Vespel
1/4" to 1/16" ferrules
1/4" To 1/8" ferrules
Ferrules
Upper temperature limit : 350
100% high-temperature DuPont polyimide
Fairly hard; tends to seal
permanently to the column
B02-2125
B02-2133
B02-2141
B02-2126
B02-2127
B02-2128
B02-2129
B02-2130
B02-2131
B02-2012
B02-2013
B02-2014
B02-2135
B02-2136
B02-2137
B02-2138
B02-2143
B02-2144
Reducing Ferrules
Capillary Column Ferrules
Straight Ferrules-for tubing with ODs as listed
Agilent style—Short ferrule, especially for Agilent inlet systems : 4890,5890 and 6890
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Ordering Information
Graphite Ferrules—Sold 10/pack Except as Noted
Cat.No.
Straight Ferrules-for tubing with ODs as listed
B02-2101
B02-2109
B02-2116
Short Ferrules for Agilent Inlets
B02-2001
B02-2002
B02-2003
B02-2004
Capillary Column Ferrules
B02-2102
B02-2103
B02-2104
B02-2105
B02-2106
Reducing Ferrules
B02-2110
B02-2111
B02-2112
B02-2113
B02-2117
B02-2118
B02-2119
B02-2120
B02-2121
B02-2122
B02-2123
B02-2124
Description
1/16" Graphite Ferrule
1/8" Graphite Ferrule
1/4" Graphite Ferrule
1/16"to 0.4mm Graphite Ferrule
1/16"to 0.5mm Graphite Ferrule
1/16"to 0.8mm Graphite Ferrule
1/16"to 1.0mm Graphite Ferrule
1/16"to 0.4mm Graphite Ferrule
1/16"to 0.5mm Graphite Ferrule
1/16"to 0.8mm Graphite Ferrule
1/16"to 1.0mm Graphite Ferrule
1/16"to 1.2mm Graphite Ferrule
1/8" to 0.4 mm Graphite Ferrule
1/8" to 0.5 mm Graphite Ferrule
1/8" to 0.8 mm Graphite Ferrule
1/8" to 1/16" Graphite Ferrule
1/4" to 1.0 mm Graphite Ferrule
1/4" to 0.4 mm Graphite Ferrule
1/4" to 0.5 mm Graphite Ferrule
1/4" to 0.8 mm Graphite Ferrule
1/4" to 1/16" Graphite Ferrule
1/4" to 1/8" Graphite Ferrule
1/4" to 6.0 mm Graphite Ferrule
1/4" to 4.0 mm Graphite Ferrule
Upper temperature limit : 450
High-purity Graphite; Preferred for capillary GC use
Ideal for glass-to-metal connections; doesn't stick to glass
Reusable if not over-tightened
Best Value
Guaranteed Product Quality
Innovation to Benefit Customers
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
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Ordering Information
Vespel/Graphite Ferrules –Sold in Packages of 10
Cat.No.
Straight Ferrules-for tubing with ODs as listed
B02-2145
B02-2153
B02-2160
Short Ferrules for Agilent Inlet
B02-2005
B02-2006
B02-2007
Capillary Column Ferrules
B02-2146
B02-2147
B02-2148
B02-2149
B02-2150
Reducing Ferrules
B02-2154
B02-2155
B02-2156
B02-2157
B02-2161
B02-2162
B02-2163
B02-2164
B02-2166
B02-2167
Description
1/16" V/G Ferrules
1/8" V/G Ferrules
1/4" V/G
1/16" to 0.4mm V/G Ferrules
1/16" to 0.5mm V/G Ferrules
1/16" to 0.8mm V/G
1/16" to 0.4mm V/G Ferrules
1/16" to 0.5mm V/G Ferrules
1/16" to 0.8mm V/G
1/16" to 1.0mm V/G Ferrules
1/16" to 1.2mm V/G
1/8" to 0.4mm V/G Ferrules
1/8" to 0.5mm V/G Ferrules
1/8" to 0.8mm V/G
1/8" to 1/16" V/G Ferrules
1/4" to 0.4mm V/G
1/4" to 0.5mm V/G
1/4" to 6.0 mm V/G Ferrules
1/4" to 0.8mm V/G Ferrules
1/4" to 1/16" V/G Ferrules
1/4" to 1/8" V/G
Upper temperature limit : 400
Made from quality DuPont polyimide/graphite blend
Recommended for GC/MS interface applications
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Ordering Information
Teflon Ferrules –Sold in Packages of 10
Cat.No.
Straight Ferrules-for tubing with ODs as listed
B02-2169
B02-2176
B02-2182
Capillary Column Ferrules
B02-2170
B02-2171
B02-2172
B02-2173
B02-2174
B02-2175
Reducing Ferrules
B02-2177
B02-2178
B02-2179
B02-2180
B02-2183
B02-2185
B02-2186
B02-2187
Description
1/16" Teflon
1/8" Teflon
1/4" Teflon
1/16" to 0.3mm Teflon
1/16" to 0.4mm Teflon
1/16" to 0.5mm Teflon
1/16" to 0.8mm Teflon
1/16" to 1.0mm Teflon
1/16" to 1.2mm Teflon
1/8" to 0.4mm Teflon
1/8" to 0.5mm Teflon
1/8" to 0.8mm Teflon
1/8" to 1/16" Teflon
1/4" to 6mm Teflon
1/4" to 1/16" Teflon
1/4" To 1/8" Teflon
1/4" to 3/16" Teflon
Upper temperature limit : 250
100% Teflon ;completely inert
Ordering Information
Cat.No.
B02-1006
Description
Column Scribe,10/pk
Column Scribe
Used to cut capillary tubing. Simply pull one smooth edge of the hard ceramic scribe (tilted about 45 degrees) across the column to lightly score
the fused silica .Bend the column opposite the score and the column separate cleanly.
Ordering Information
Cat.No.
B02-4001
B02-4002
B02-4003
B02-4004
B02-4005
B02-4006
Description
1/32" Union,Bore 0.25mm
1/32" Union,Bore 0.50mm
1/32" Union,Bore
1/32" 1/32" Fused Silica Tubing Adapter Tubing o.d.<0.25mm
1/32" Fused Silica Tubing Adapter Tubing o.d. 0.25-0.4mm
1/32" Fused Silica Tubing Adapter Tubing o.d.0.5mm
1/32” Ultra Low Mass External Unions
For capillary tubing 0.1 to 0.32mm I.D. It has very low mass and can be hand-tightened to seal .Use only with 1/32” fused silica adapters; metal
ferrules cannot be used.
Best Value
Guaranteed Product Quality
Innovation to Benefit Customers
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
AgelaTechnologies
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Syringe Filters
Filtration Products
Ordering Information
Mixed cellulose (acetate and nitrate) syringe filters
Nylon syringe filters
13mm Syringe Filters 0.2μm 100 AS011320
0.45μm 100 AS011345
25mm Syringe Filters 0.2μm 100 AS012520
0.45μm 100 AS012545
13mm Syringe Filters with glass prefilter 0.2μm 100 AS011320-G
0.45μm 100 AS011345-G
25mm Syringe Filters with glass prefilter 0.2μm 100 AS012520-G
0.45μm 100 AS012545-G
13mm Syringe Filters 0.2μm 100 AS021320
0.45μm 100 AS021345
25mm Syringe Filters 0.2μm 100 AS022520
0.45μm 100 AS022545
13mm Syringe Filters with PP prefilter 0.2μm 100 AS021320-P
0.45μm 100 AS021345-P
25mm Syringe Filters with PP prefilter 0.2μm 100 AS022520-P
0.45μm 100 AS022545-P
PVDF syringe filters
13mm Syringe Filters 0.2μm 100 AS031320
0.45μm 100 AS031345
25mm Syringe Filters 0.2μm 100 AS032520
0.45μm 100 AS032545
13mm Syringe Filters with PP prefilter 0.2μm 100 AS031320-P
0.45μm 100 AS031345-P
25mm Syringe Filters with PP prefilter 0.2μm 100 AS032520-P
0.45μm 100 AS032545-P
PTFE syringe filters
13mm Syringe Filters 0.2μm 100 AS041320
0.45μm 100 AS041345
25mm Syringe Filters 0.2μm 100 AS042520
0.45μm 100 AS042545
1μm 100 AS042501
5μm 100 AS042505
13mm Syringe Filters with PP prefilter 0.2μm 100 AS041320-P
0.45μm 100 AS041345-P
25mm Syringe Filters with PP prefilter 0.2μm 100 AS042520-P
0.45μm 100 AS042545-P
1μm 100 AS042501-P
5μm 100 AS042505-P
High Quality – All of our products are manufactured by ISO 9001 registered manufacturers and are guaranteed to meet our
published specification.
Best Value – Our list prices are significantly lower than most brand name prices in the market.
Qty. Cat No.
Accessories/S
upplie
s
163
Ordering Information
Polyethersulfone (PES) syringe filters
Regenerated cellulose (RC) syringe filters
13mm Syringe Filters 0.2μm 100 AS051320
0.45μm 100 AS051345
25mm Syringe Filters 0.2μm 100 AS052520
0.45μm 100 AS052545
13mm Syringe Filters with glass prefilter 0.2μm 100 AS051320-G
0.45μm 100 AS051345-G
25mm Syringe Filters with glass prefilter 0.2μm 100 AS052520-G
0.45μm 100 AS052545-G
13mm Syringe Filters 0.2μm 100 AS061320
0.45μm 100 AS061345
25mm Syringe Filters 0.2μm 100 AS062520
0.45μm 100 AS062545
13mm Syringe Filters with glass prefilter 0.2μm 100 AS061320-G
0.45μm 100 AS061345-G
25mm Syringe Filters with glass prefilter 0.2μm 100 AS062520-G
0.45μm 100 AS062545-G
The Syringe Filters’ Specifications
Description
MCM
Nylon
PVDF
PTFE
13
13
25
25
13
13
25
25
13
13
25
25
13
13
25
25
0.22
0.45
0.22
0.45
0.22
0.45
0.22
0.45
0.22
0.45
0.22
0.45
0.22
0.45
0.22
0.45
7
7
5
5
7
7
5
5
7
7
5
5
7
7
5
5
3.52
2.20
3.52
2.20
2.80
2.50
2.80
2.50
1.20
0.56
1.20
0.56
1.00
0.63
1.00
0.63
18
60
18
60
25
75
25
75
15
35
15
35
24
60
24
60
0.65
0.65
3.90
3.90
0.65
0.65
3.90
3.90
0.65
0.65
3.90
3.90
0.65
0.65
3.90
3.90
16
20
16
20
5
8
5
8
Diameter of
the filter(mm)
Pore size
(μm)
Pressure
rating(bar)
Bubble Point
rating(bar)
Water speed
(ml/min/cm2)
Air speed
(L/min/cm2)
Effective
filter area(cm2)
Best Value
Guaranteed Product Quality
Innovation to Benefit Customers
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
Cat No. Qty
AgelaTechnologies
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Ordering Information
Membrane Filters
Material: cellulose acetate, nylon
Pore size: 0.45μm
Filter size: 13mm, 25mm, 50mm
Customized sizes available upon
request
Material Diameter(mm) Qty Cat. No.
13
25
50
13
25
50
Cellulose Acetate
Nylon
100/pk
100/pk
100/pk
100/pk
100/pk
100/pk
AM011345
AM012545
AM015045
AM021345
AM022545
AM025045
Glass Filtration System
Funnel Volume (mL) Flask Volume (mL) Qty Cat. No.
Ordering InformationFunnel volume: 300mL, 500mL
Pore size of the glass support: 10μm
Flask volume: 1000ml, 2000mL1000
2000
1000
2000
300
300
500
500
ea
ea
ea
ea
AM3100
AM3200
AM5100
AM5200
The system includes a borosilicate glass funnel, a sintered borosilicate glass membrane support base on neck, aluminum clamp and borosili-
cate glass filter flask. It can be used with or without membrane depending on the application requirement to filtrate organic or aqueous solu-
tion. It is also used for liquid degassing.
Ordering Information
Type Vol Qty Cat.No.
Filtration plate
PP frits 20µm
Filtration plate
PTFE membrane 0.45µm
Filtration plate packed with
earth diatomaceous 100mg
Protein precipitation plates
and collection
Collection plate, round bottom
96 well cap mats
2mL
1mL
2mL
1mL
2mL
1mL
2mL
1mL
2mL
1mL
—
10
10
10
10
10
10
10
10
10
10
10
FTPP2002-01
FTPP2001-01
FTPF4502-01
FTPF4501-01
FTFT1002-01
FTFT1001-01
PPPC1002-01
PPPC1001-01
CLCM0002-01
CLCM0001-01
CLCM9600-0
96 Well Filtration Plates and Collection Plates
Accessories/S
upplie
s
165
Chemical Mixed Cellulose Acetate/nitrate Regenerate Cellulose Nylon PTEE
1-hexanol
Acetic acid,10%&25%
Acetone
Acetonitrile
Aliphatic hydrocarbon
Ammonia,1M
Aromatic hydrocarbon
Benzene
Boric acid
CTC
Carboxylic acid
Chloroacetic acid
Chloroform
Cyclohexane
Cyclohexanol
Diethyl ether
Dimethyl formamide
DMSO
Alcohol 98%
Acetic ether
Vinyl chloride
Ethylene glycol
Formic acid, 25%
N-hexane
Hydrochloric acid,25%
Isopropyl alcohol
Methanol
Nitrous acid,25%
Pentane
Phosphoric acid,25%
Phosphoric acid,45%
KOH,1M
Water solution of inorganic salt
NaOH,1M
Acetylene tetrachloride
THF
Toluene
TCA,10%
Trichloro ethane
Trichloroethylene
Dimethyl benzene
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
N/A
0
N/A
0
0
0
0
0
0
0
Chemical Resistance Guide for Membranes
The mark represent: “ ” = chemical resistance; “0” = limited chemical resisitant; “ ” = no chemical resistance ; N/A= no data.
Best Value
Guaranteed Product Quality
Innovation to Benefit Customers
Ordering Phone No.: (302) 438 8798
Support Phone No.: (302) 588 4556
AgelaTechnologies
BETTER SOLUTION FOR CHROMATOGRAPHY
Accessories/S
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166
2 mL 12 x 32 mm Screw Neck Vials and Caps
Vials and Caps
Vials
Agela Technologies Inc offers a number of high quality vials, caps, seals and crimping tools. Most of them are manufactured in Europe and
meet the high standard of the chromatographic applications. The difference of our offering from many other brand name venders is the cost to
you. We offer lower price for the same products as many brand name’ s due to our lean cost structure and we provide personal assist to you to
select right products
Description Thread Color Cat. No.(100/pk) Cat. No.(1000/pk)
Std
Std
Std
Std
short
short
short
Wide mouth 10/425
Wide mouth 10/425
Wide mouth 10/425
Clear
Amber
Clear
Amber
Clear
Clear
Amber
Clear
Clear
Amber
AV1000-0
AV1010-0
AV1001-0
AV1011-0
AV1100-0
AV1101-0
AV1111-0
AV1200-0
AV1201-0
AV1211-0
AV1000-1
AV1010-1
AV1001-1
AV1011-1
AV1100-1
AV1101-1
AV1111-1
AV1200-1
AV1201-1
AV1211-1
Screw neck vials
Screw neck vials
Screw neck vials, graduated
Screw neck vials, graduated
Screw neck vials
Screw neck vials, graduated
Screw neck vials, graduated
Screw neck vials
Screw neck vials, graduated
Screw neck vials, graduated
Description Thread Cat. No.(100/pk) Cat. No.(1000/pk)
Caps and Septa
Std
short
Wide mouth (10/425)
AV2000-0
AV2100-0
AV3100-0
AV2000-1
AV2100-1
AV3100-1
Screw cap
Screw cap
Screw cap (wide mouth)
2 mL 12 x 32 mm Crimp Neck Vials and Caps
Vials
Description Thread Color Cat. No.(100/pk) Cat. No.(1000/pk)
Description Thread Cat. No.(100/pk) Cat. No.(1000/pk)
Caps and Septa
Narrow mouth
Narrow mouth
Wide mouth
Narrow mouth
Narrow mouth
Wide mouth
Wide mouth
Clear
Amber
Clear
Clear
Amber
Clear
Amber
AV5000-0
AV5010-0
AV5001-0
AV5100-0
AV5101-0
AV5111-0
AV5200-0
AV5000-1
AV5010-1
AV5001-1
AV5100-1
AV5101-1
AV5111-1
AV5200-1
Crimp neck vials
Crimp neck vials
Crimp neck vials
Crimp neck vials, graduated
Crimp neck vials, graduated
Crimp neck vials, graduated
Crimp neck vials
Narrow mouth
Wide mouth
AV6000-0
AV6100-0
AV6000-1
AV6100-1
Crimp cap
Crimp cap
Plastic Vials (micro-vials for LC-MS autosamplers)
Description Material Vol. Cat. No.
PP
TPX
PP
Silicone/PTFE
300µL
300µL
—
—
AV0003-P
AV0003-T
AV0003-C
AV0003-S
Snap ring micro-vial32×11.6mm
Snap ring micro-vial32×11.6mm
Snap Cap
Septa