AfCD1
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AfCD1 AfCD2 AfCD3 AfCI1 AfCI2 WC3 AfCI3 AfTD1 WC1 WC2 AfTD2 AfTD3 AfTI1 AfTI2 AfTI3 WT1 WT2 WT3 (a ) AfCD1 AfCD2 AfCD3 AfCI1 AfCI2 WC3 AfCI3 AfTD1 WC1 AfTD2 AfTD3 AfTI1 AfTI2 AfTI3 WT1 WT2 (b ) Supplemental Figure S1
description
AfTD1. AfTI1. AfCI3. AfTD2. (a). AfTD3. (b). AfCD3. AfCD1. AfTD2. AfCD2. AfTD3. AfCI2. AfCD3. AfTI2. AfTD1. AfTI3. AfCD1. WC1. AfCI1. AfCD2. WC3. AfCI3. WT1. AfTI2. WT2. AfCI1. AfTI1. AfTI3. WT3. WC1. WT1. AfCI2. WC3. WT2. WC2. Supplemental Figure S1. (c). - PowerPoint PPT Presentation
Transcript of AfCD1
AfCD1
AfCD2
AfCD3
AfCI1
AfCI2
WC3
AfCI3
AfTD1
WC1
WC2
AfTD2
AfTD3
AfTI1
AfTI2
AfTI3
WT1
WT2
WT3
(a)
AfCD1
AfCD2
AfCD3
AfCI1
AfCI2
WC3
AfCI3
AfTD1
WC1
AfTD2
AfTD3
AfTI1
AfTI2
AfTI3
WT1
WT2
(b)
Supplemental Figure S1
AfCD1
AfCD2
AfCD3
AfCI1
AfCI2
WC3
AfCI3
AfTD1
WC1
WC2
AfTD2
AfTD3
AfTI1
AfTI2
AfTI3
WT1
WT2
WT3
(c)
p=0.718 p=0.096
(a)
p=0.718
(b)
p=0.096 p=0.718 p=0.096 p=0.718 p=0.096
(a)
p=0.718
(a)
p=0.718
(b)
p=0.096
(b)(b)
p=0.096
Supplemental Figure S2
Supplemental Figure S3
Similarity Observed OTUs Average Low High97% 79 165.125 120.664 257.03495% 66 101 81.2932 146.101
Chao1
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Number of analyzed sequences
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Supplemental material:• Supplemental Figure S1: Cluster analysis using the unweighted-pair group
method for (a) total bacterial and (b) Pseudomonas and (c) Actinobacteria. AfCD, A. fulva from Caboclo Island extracted directly; AfCI, A. fulva from Caboclo Island extracted indirectly; AfTD, A. fulva from Tartaruga beach extracted directly; AfTI, A. fulva from Tartaruga beach extracted indirectly; SWC, surrounding seawater from Caboclo Island; SWT, surrounding seawater from Tartaruga beach.
• Supplemental Figure S2: LIBSHUFF comparison plots of evolutionary distance by homologous coverage curves from direct (red line) compared to heterologous coverage curve from indirect (green line) 16S rRNA gene clone libraries (a) and homologous coverage curves for indirect (red line) compared to heterologous coverage curve direct (green line) 16S rRNA gene clone libraries (b). The blue line indicates the value of delta C (right y axis) for the original samples at each value of evolutionary distance. Purple line indicates the 95% of delta C for the randomized samples. The ranked values (p) of the delta C from the heterologous coverage are shown.
• Supplemental Figure S3: Rarefaction curves of observed operational taxonomic units (OTU) at the “species” (97%, bold) and “genus” (95%, dotted) levels for partial bacterial 16S rRNA gene sequences retrieved from A. fulva derived DNA. The table on the upper-left side shows Chao1 richness estimations with their respective low and high confidence limits (95%), as well as the number of OTUs determined with DOTUR for each case evaluated.
