Aerobic Gram Negative Cocci

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Aerobic Gram Negative Cocci Student Lab Division of Medical Technology Jeanne Filbey, MT(ASCP)

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Aerobic Gram Negative Cocci. Student Lab Division of Medical Technology Jeanne Filbey, MT(ASCP). Aerobic GNDC. General Characteristics. Normal flora of upper respiratory, alimentary and genitourinary tracts Gram stain: GNDC Oxidase positive Catalase positive Non-motile. Fastidious - PowerPoint PPT Presentation

Transcript of Aerobic Gram Negative Cocci

Page 1: Aerobic Gram Negative Cocci

Aerobic Gram Negative Cocci

Student LabDivision of Medical Technology

Jeanne Filbey, MT(ASCP)

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General Characteristics• Normal flora of upper respiratory,

alimentary and genitourinary tracts • Gram stain: GNDC• Oxidase positive• Catalase positive• Non-motile

Aerobic GNDC

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Neisseria gonorrhoeae• Fastidious• Temperature sensitive• Culture immediately• Transport media if delay

Aerobic GNDC

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Specimen Gram Stain• Intracellular GNDC

Neisseria gonorrhoeae

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Colony Morphology• Chocolate agar

• Thayer-Martinagar– Growth– Contains antibiotics

Neisseria gonorrhoeae

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Identification• Carbohydrates

– Glucose pos– Maltose neg– Lactose neg– Sucrose neg

• DNase neg• Nitrate neg• Butyrate esterase neg

Neisseria gonorrhoeae

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Pathogenesis• Port of entry• Progression of infection• Human to human transfer• Females asymptomatic

Neisseria gonorrhoeae

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Gonorrhea• Male

– Acute urethritis, prostatitis and epididymitis if untreated

• Female– May be asymptomatic– Cervicitis --> pelvic inflammatory disease --

> scarring of fallopian tubes --> sterility, ectopic pregnancies

Neisseria gonorrhoeae

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Other Infections• Disseminated gonococcal infection• Pharyngitis• Gonococcal ophthalmia neonatorum• Vulvovaginitis

Neisseria gonorrhoeae

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Treatment• Antimicrobial testing

Neisseria gonorrhoeae

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Neisseria meningitidis• Fastidious• Temperature sensitive• Culture immediately• Occasionally grows on SBA

Aerobic GNDC

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Specimen Gram Stain• GNDC

Neisseria meningitidis

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Colony Morphology• Chocolate agar

• Thayer-Martinagar– Growth

Neisseria meningitidis

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Identification• Carbohydrates

– Glucose pos– Maltose pos– Lactose neg– Sucrose neg

• DNase neg• Nitrate neg• Butyrate esterase neg

Neisseria meningitidis

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Pathogenesis• Meningitis• Septicemia• Septic arthritis• Endocarditis• Pneumonia• Urethritis• Asymptomatic nasopharyngeal carriers

Neisseria meningitidis

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Neisseria lactamica• Normal flora

Aerobic GNDC

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Colony Morphology• Chocolate agar

• Thayer-Martinagar– Growth

Neisseria lactamica

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Identification• Carbohydrates

– Glucose pos– Maltose pos– Lactose pos– Sucrose neg

• DNase neg• Nitrate neg• Butyrate esterase neg

Neisseria lactamica

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Moraxella (Branhamella) catarrhalis• Can be normal respiratory flora

Aerobic GNDC

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Colony Morphology• Sheep blood agar• Chocolate agar• Thayer-Martin

agar– Growth

Moraxella (Branhamella) catarrhalis

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Identification• Carbohydrates

– Glucose neg– Maltose neg– Lactose neg– Sucrose neg

• DNase pos• Nitrate pos• Butyrate esterase pos

Moraxella (Branhamella) catarrhalis

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Pathogenesis• Acute bronchitis• Pneumonia• Meningitis• Seen in immunocompromised patients

(elderly)• Otitis media & maxillary sinusitis in kids• Most produce beta lactamase

Moraxella (Branhamella) catarrhalis

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Summary• Specimen handling and processing• Growth characteristics• Identification• Clinical significance• Antimicrobial testing

Aerobic GNDC

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Who am I?

Urethral gram stain Thayer Martin agar

Neisseria gonorrhoeae

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Who am I?

CSF gram stain Chocolate agar

Neisseria meningitidis