Aerobic Gram Negative Cocci
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Transcript of Aerobic Gram Negative Cocci
Aerobic Gram Negative Cocci
Student LabDivision of Medical Technology
Jeanne Filbey, MT(ASCP)
General Characteristics• Normal flora of upper respiratory,
alimentary and genitourinary tracts • Gram stain: GNDC• Oxidase positive• Catalase positive• Non-motile
Aerobic GNDC
Neisseria gonorrhoeae• Fastidious• Temperature sensitive• Culture immediately• Transport media if delay
Aerobic GNDC
Specimen Gram Stain• Intracellular GNDC
Neisseria gonorrhoeae
Colony Morphology• Chocolate agar
• Thayer-Martinagar– Growth– Contains antibiotics
Neisseria gonorrhoeae
Identification• Carbohydrates
– Glucose pos– Maltose neg– Lactose neg– Sucrose neg
• DNase neg• Nitrate neg• Butyrate esterase neg
Neisseria gonorrhoeae
Pathogenesis• Port of entry• Progression of infection• Human to human transfer• Females asymptomatic
Neisseria gonorrhoeae
Gonorrhea• Male
– Acute urethritis, prostatitis and epididymitis if untreated
• Female– May be asymptomatic– Cervicitis --> pelvic inflammatory disease --
> scarring of fallopian tubes --> sterility, ectopic pregnancies
Neisseria gonorrhoeae
Other Infections• Disseminated gonococcal infection• Pharyngitis• Gonococcal ophthalmia neonatorum• Vulvovaginitis
Neisseria gonorrhoeae
Treatment• Antimicrobial testing
Neisseria gonorrhoeae
Neisseria meningitidis• Fastidious• Temperature sensitive• Culture immediately• Occasionally grows on SBA
Aerobic GNDC
Specimen Gram Stain• GNDC
Neisseria meningitidis
Colony Morphology• Chocolate agar
• Thayer-Martinagar– Growth
Neisseria meningitidis
Identification• Carbohydrates
– Glucose pos– Maltose pos– Lactose neg– Sucrose neg
• DNase neg• Nitrate neg• Butyrate esterase neg
Neisseria meningitidis
Pathogenesis• Meningitis• Septicemia• Septic arthritis• Endocarditis• Pneumonia• Urethritis• Asymptomatic nasopharyngeal carriers
Neisseria meningitidis
Neisseria lactamica• Normal flora
Aerobic GNDC
Colony Morphology• Chocolate agar
• Thayer-Martinagar– Growth
Neisseria lactamica
Identification• Carbohydrates
– Glucose pos– Maltose pos– Lactose pos– Sucrose neg
• DNase neg• Nitrate neg• Butyrate esterase neg
Neisseria lactamica
Moraxella (Branhamella) catarrhalis• Can be normal respiratory flora
Aerobic GNDC
Colony Morphology• Sheep blood agar• Chocolate agar• Thayer-Martin
agar– Growth
Moraxella (Branhamella) catarrhalis
Identification• Carbohydrates
– Glucose neg– Maltose neg– Lactose neg– Sucrose neg
• DNase pos• Nitrate pos• Butyrate esterase pos
Moraxella (Branhamella) catarrhalis
Pathogenesis• Acute bronchitis• Pneumonia• Meningitis• Seen in immunocompromised patients
(elderly)• Otitis media & maxillary sinusitis in kids• Most produce beta lactamase
Moraxella (Branhamella) catarrhalis
Summary• Specimen handling and processing• Growth characteristics• Identification• Clinical significance• Antimicrobial testing
Aerobic GNDC
Who am I?
Urethral gram stain Thayer Martin agar
Neisseria gonorrhoeae
Who am I?
CSF gram stain Chocolate agar
Neisseria meningitidis