Advancements in Rodent Surgical Monitoring
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Transcript of Advancements in Rodent Surgical Monitoring
InsideScientific is an online educational environment designed for life science researchers.
Our goal is to aid in the sharing and distribution of scientific information regarding innovative technologies, protocols,
research tools and laboratory services.
Graham Sattler Rodent surgical monitoring
advocate, applications specialist, and Product
Manager for Indus biomedical solutions
Corey Reynolds PhD Director of the Mouse
Phenotyping Core Lab at Baylor College of Medicine,
advanced murine model surgeon, IACUC and animal
care expert
Merry Lindsey, PhD Director of the Jackson
Center for Heart Research at The University of Mississippi,
celebrated professor, and expert in murine models of
myocardial infarction
Audience Feedback 0% 10% 20% 30% 40% 50% 60% 70% 80%
Strip Recording
Photos of Surgical Setup
Recording on Data Acquisition System
Lab Notebook
How do you currently document your surgery for method consistency?
Surgical Monitoring Overview
What can Surgical Monitoring do for Me?
MouseMonitor S Capabilities
Critical Surgical Monitoring Signals
Integrated Surgical Monitoring Doesn’t Have to Be Hard
What Can Surgical Monitoring Do For Me?
Consistent Outcomes
Consistent Procedures
Increased Survival Rate
Better Surgical Training
Surgical Monitoring Essentials…
Non-Invasive 6-Lead ECG
Blood Oxygenation Monitoring
Respiration Monitoring
Responsive Heating
Core Temperature Measurement
Multiple physiologic signals must be monitored to effectively assess subject health
Monitor…
Heart Rate
Core Temperature
Blood Oxygenation
Maintain…
Safe Core Temperature
Critical Surgical Monitoring Signals
ECG, Heart Rate & Respiration Rate from a single set of electrodes
Perform Surgeries directly on Stainless Steel top
Intuitive Touch Screen Controls
Factory Presets Enable Out of Box Operation
All Signals Captured Non-Invasively
…it doesn’t have to be hard
Signal Integration…
Comprehensive monitoring offers greater consistency and confidence
Consistent Interventions + Higher Survival Rate = Better Outcomes
The MouseMonitor S is the only preclinical research device that measures every key vital sign in one device
Rodent Surgical Monitoring In Review
Click Here For More Information
Audience Feedback 0% 10% 20% 30% 40% 50% 60%
Somewhat Important
Important
Very Important
How important do you view rodent surgical monitoring?
The Mouse Phenotyping Core is a comprehensive facility offering expertise in the following areas:
What does the MPC Offer?
Imaging
Metabolic Assays
Cardiovasuclar Assays
Respiratory Assays
Neuromuscular Assessment
Surgical services
MPC Cont.
Fee-for-service Core located at BCM
Can accept animals (rats/mice) from outside facilities post veterinary approval
Can run long term studies and provide data analysis
Contact Information: Corey L. Reynolds
What does the MPC Offer?
Many Different
Species
Clients/PIs
Protocols
Procedures
Challenges in a Core Lab…
Guide for the Care and Use of Laboratory Animals , 8th ed.
Recommendations By The NRC
Providing Artificial Heating (p119)
Monitoring baseline vital signs, including:
Heart Rate (p119) Core Temperature (p119) Blood Oxygenation (p122)
Core temperature monitoring &
adjustable heating.
ECG/Heart Rate electrode
flexibility for different-sized
species.
Blood Oxygenation
(SpO2) monitoring.
Compact Comprehensive
Surgical monitoring.
What a Core Lab Needs…
Easy Screenshots
Easy Recordings
Advantages of Integrated Monitoring & Recording
Constant Monitoring of Animal Vitals
During Surgery
Documentation of Monitoring
Very Liked by IACUC
committee.
Advantages of Integrated Monitoring & Recording
Multiple Hi-Resolution ECG leads enable easier Morphology Recognition & Arrhythmia Detection
Sinus Rhythm with Bigeminal PVCs
Applications In The Mouse Phenotyping Core
Serial Ultrasound (Vevo) B-Mode and ECG capture for analysis
ECG data integrates effectively with other equipment as well
Applications In The Mouse Phenotyping Core
Audience Feedback 0% 5% 10% 15% 20% 25% 30% 35%
My current setup doesn’t work properly
I don’t currently have suitable monitoring
equipment
I am working on setting up my lab
My current setup takes too much time to use
What factors influence how frequently you utilize surgical monitoring?
Universal definition of myocardial infarction. Kristian Thygesen, Joseph S.
Alpert and Harvey D. White on behalf of the Joint ESC/ACCF/AHA/WHF Task Force for the Redefinition of Myocardial Infarction.
Eur Heart J (2007) 28 (20): 2525-2538.
Myocardial Infarction (by Left Coronary Artery Decending (LAD) Ligation)C
Yabluchanskiy et al. Congest Heart Fail. 2013
Post-MI Cardiac Remodeling
Effects of MMP-9 deletion in aging mice post-MI
Effects of MMP-9 deletion post-MI in mice with P. gingivalis infection
Effects of MMPs derived peptides in vitro and in vivo
Effects of selective MMP inhibitors post-MI
Lindsey Lab – Current Research Areas
ECG Before LAD Ligation
ECG 5 Min After LAD Ligation
Reliable & accurate ECG recording
Multiple leads choice
External and internal ECG electrodes
Controlled heated pad
Critical Needs in our Lab For In Vivo Models
Comfortable size of the pad
External temperature probe
Portable tablet system
Data management/export
Thank you for taking part in this event. We encourage all attendees to register at www.insidescientific.com for notifications about future webinars.
WEBSERIES COMING SOON… PV Loops to Measure Cardiac Function
Implantable Biotelemetry
Non-Invasive Blood Pressure Monitoring
