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DOI 10.13350 j.cjpb.180314 ·!"· ·dp»pAB¼½GHl¾¿ÀÁÂ| ÓÔb ÕÖÔ ×bØ ÙÚ Û| Ü 1. ýÒ+&,ð¡ñ&àà;±1ýÒ 541004 2.''þ+%&Yëγ¡¸&àà;!"#$ vw[@Ýhñ67$6EP_°4áÙKU¿%& Y C57BL B BALB 'í®i;V,A1000050000J9[³ñ狅犲犾犻犻 17XNL ?"?3hwÞ¾¿hijK ¡( Î;',Aw30 90 180dN10000"A[³ñ?"?QR%&-5EP(Î;ý"#·PY±;G ZiQR%·4jG-[$6EPÇ4Zi|\·K»¼`'( [³ññ·³$6w 30d<A'Ýå100%K$6EP$6w90d C57BL EP÷å]á180d#EP´40% Å BALB 'EP1ÈÊæ!C57BL ' 4 CD8 EM Z i PD1 K ý $ 6 w 180d Ê"Õ £ =7.369 0.01 ), BALB '1ÈÊæ!=1.368 0.05 )。 C57BL '4 CD8 EM Zi PD1K$6w_ÖÅ£{¥n$6w180d90d#Õ£¦§ÕÊ"=6.175 0.01 )。 ') ý C57BL '[³ñÝ hñ67$6EP_$6#·l°4áÙÇ4 CD8 Zi PD1¼*+,[³ñJ967CD8 ZiPD1 -./01R382.31 23456278116735234 2018 03027804 犑狅狌狉狀犪犾狅 犘犪狋犺狅 犲狀犅犻狅犾狅 犵狔.2018Mar 13 ): 278-281 286. 犃狆狉犲犾犻犿犻狀犪狉狔狊狋狌犱狔狅犳狋犺犲犿犲犮犺犪狀犻狊犿犳狅狉狉犲犱狌犮犲犱犲犳犳犻犮犪犮狔狅犳犪狀犱狆狉狅狋犲犮狋犻狅狀犫狔犪犿犪犾犪狉犻犪狏犪犮犮犻狀犲 JIANGLinping GUANHongli TENGPingying MO Gang LIDayu PENG Xiaohong 犇犲 犪狉狋犿犲狀狋狅 犘犪狉犪狊犻狋狅犾狅 犵狔 犌狌犻犾犻狀 犕犲犱犻犮犪犾犝狀犻狏犲狉狊犻狋 犌狌犻犾犻狀 犌狌犪狀 狓犻541004 犆犺犻狀犪 犇犲 犪狉狋犿犲狀狋狅 犘犪狋犺狅 犲狀犅犻狅犾狅 犵狔 犜犺犻狉犱犕犻犾犻狋犪狉 犕犲犱犻犮犪犾犝狀犻狏犲狉狊犻狋 犃犫狊狋狉犪犮狋 犗犫犼犲犮狋犻狏犲狊 Toinvestigatethe mechanismforreducedefficacyofandprotectionbya malariavaccine. 犕犲狋犺狅犱狊 C57BL 6orBALB cmicewereintravenouslyinoculated3timeswith10kor50k WT犘犾犪狊犿狅犱犻狌犿 狅犲犾犻犻spo rozoites spz at4weekintervals.Threehoursafterinjection themiceweretreated with0.8 mgofchloroquine CQ for10consecutivedays.Micewerechallengedwith10k WTspzthatwereinjectedintravenously30 90 or180dafter immunization.Thephenotypeofhepaticlymphocyteswasdeterminedwithflowcytometry. 犚犲狊狌犾狋狊 BothC57BL 6and BALB cmicehad100% protection30dafterimmunization.However 10% ofC57BL 6mice 10 haddetectable bloodstageparasitemia90dafterimmunization and60%ofC57BL 6mice 10 hadparasitemia180dafterimmuniza tion.AlloftheBALB cmicehad100%protection180dafterimmunization.Phenotypicanalysisoflymphocytesindica tedthatPD1expressionon CD8 TcellsincreasedsignificantlyinC57BL 6 mice90dafterimmunizationbutnotin BALB cmice.Moreover thelevelofPD1expressioninmice180dafterimmunizationwassignificantlyhigherthanthat inmice90dafterimmunization =6.175 0.01 犆狅狀犮犾狌狊犻狅狀 Thereducedefficacyofandprotectionbymalaria vaccinewascorrelatedwithahighlevelofPD1expressiononCD8 Tcells. 犓犲狔狑狅狉犱狊 犘犾犪狊犿狅犱犻狌犿 狅犲犾犻犻 vaccine CD8 Tcells PD1 [@乬-Õ p c Kij @ª( WHO k]2015 ñ J â 42.9 £ m3[@¥nJ30.3£mpÔf]LM ä ¹(¶·[Ø R ýÏ# ´ Àí Íð( ' K · "#rñx ñ t K68 8k [ @ 67 ¿Õp` [³ñÝhñ67rÔxx?"?% &K$6E P Å ¥ s. â càá Í,AqZ? " ?mÖ¥ $ 6 E P À N ^ s .10WXàá º p Ý h 67 E P K ï s Á³Ks. F ýx E P ª j K $ 6 U¿µ=;¬-Ýh67KEP5_?5 _ CD8 Z iCD8 EM ÔCD8 EM Zi+ Ç x K E P d * Û Å àá  Í$6w_#·l x E P á Ù [ ³ñ @ · · . /0123456 犑狅狌狉狀犪犾狅 犘犪狋犺狅 犲狀犅犻狅犾狅 犵狔 2018ñò '13ó'h March2018 Vol.13 No.3 9:;#± 1 H / & Y ô õ ä No. 2017GXNSFAA198229 ); ± 1{ é n Ø ñYë [ + £ õä No.2017KY0491 )。 <=>? Email pxh815 163.com >?@AîA01981- ), V± 1 ý Ò mûüW æð¡ñijÇ$6Email 52972865 qq.com

Transcript of •dp»pAB…‰GHl¾¿À`´|cjpb.org/pdfup/2018042505895329.pdf · 2018-04-25 ·...

Page 1: •dp»pAB…‰GHl¾¿À`´|cjpb.org/pdfup/2018042505895329.pdf · 2018-04-25 · globulindomainandmucindomain3,TIM3)B„Ø ‹Zi3ˇœß1(Programmedcelldeathprotein1, PD1)K|¥æ!,9z{Ž1。

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[犑狅狌狉狀犪犾狅犳犘犪狋犺狅犵犲狀犅犻狅犾狅犵狔.2018Mar;13(3):278-281,286.]

犃狆狉犲犾犻犿犻狀犪狉狔狊狋狌犱狔狅犳狋犺犲犿犲犮犺犪狀犻狊犿犳狅狉狉犲犱狌犮犲犱犲犳犳犻犮犪犮狔狅犳犪狀犱狆狉狅狋犲犮狋犻狅狀犫狔犪犿犪犾犪狉犻犪狏犪犮犮犻狀犲

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 (1.犇犲狆犪狉狋犿犲狀狋狅犳犘犪狉犪狊犻狋狅犾狅犵狔,犌狌犻犾犻狀犕犲犱犻犮犪犾犝狀犻狏犲狉狊犻狋狔,犌狌犻犾犻狀,犌狌犪狀犵狓犻541004,犆犺犻狀犪;2.犇犲狆犪狉狋犿犲狀狋狅犳

犘犪狋犺狅犵犲狀犅犻狅犾狅犵狔,犜犺犻狉犱犕犻犾犻狋犪狉狔犕犲犱犻犮犪犾犝狀犻狏犲狉狊犻狋狔)

【犃犫狊狋狉犪犮狋】 犗犫犼犲犮狋犻狏犲狊 Toinvestigatethemechanismforreducedefficacyofandprotectionbyamalariavaccine. 

犕犲狋犺狅犱狊 C57BL/6orBALB/cmicewereintravenouslyinoculated3timeswith10kor50kWT犘犾犪狊犿狅犱犻狌犿狔狅犲犾犻犻spo

rozoites(spz)at4weekintervals.Threehoursafterinjection,themiceweretreatedwith0.8mgofchloroquine(CQ)

for10consecutivedays.Micewerechallengedwith10kWTspzthatwereinjectedintravenously30,90,or180dafter

immunization.Thephenotypeofhepaticlymphocyteswasdeterminedwithflowcytometry. 犚犲狊狌犾狋狊 BothC57BL/6and

BALB/cmicehad100% protection30dafterimmunization.However,10% ofC57BL/6mice(1/10)haddetectable

bloodstageparasitemia90dafterimmunization,and60%ofC57BL/6mice(6/10)hadparasitemia180dafterimmuniza

tion.AlloftheBALB/cmicehad100%protection180dafterimmunization.Phenotypicanalysisoflymphocytesindica

tedthatPD1expressiononCD8+TcellsincreasedsignificantlyinC57BL/6mice90dafterimmunizationbutnotin

BALB/cmice.Moreover,thelevelofPD1expressioninmice180dafterimmunizationwassignificantlyhigherthanthat

inmice90dafterimmunization(狋=6.175,犘<0.01). 犆狅狀犮犾狌狊犻狅狀 Thereducedefficacyofandprotectionbymalaria

vaccinewascorrelatedwithahighlevelofPD1expressiononCD8+Tcells.

【犓犲狔狑狅狉犱狊】 犘犾犪狊犿狅犱犻狌犿狔狅犲犾犻犻;vaccine;CD8+Tcells;PD1

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March2018,Vol.13,No.3