ADP, ATP and ADP, ATP and Cellular Cellular

RespirationRespiration

What Is ATP?What Is ATP?

Energy used by all CellsEnergy used by all Cells

Adenosine TriphosphateAdenosine Triphosphate

Organic molecule containing Organic molecule containing high-energy Phosphate bondshigh-energy Phosphate bonds

Chemical Structure of Chemical Structure of ATPATP

3 Phosphates

Ribose Sugar

Adenine Base

What Does ATP Do for What Does ATP Do for You?You?

It supplies YOU withIt supplies YOU with ENERGY!ENERGY!

How Do We Get Energy How Do We Get Energy From ATP?From ATP?

By breaking By breaking the high- the high- energy energy bonds bonds between between the the last two last two phosphates phosphates in ATPin ATP

What is the Process What is the Process Called?Called?

HYDROLYSIS (Adding HHYDROLYSIS (Adding H22O)O)

H2O

How Does That How Does That Happen?Happen?

An An Enzyme!Enzyme!

How is ATP Re-Made?How is ATP Re-Made?

The reverse of the previous The reverse of the previous process occurs.process occurs.

Another Another Enzyme is Enzyme is used!used!ATP ATP SynthetaseSynthetase

The ADP-ATP CycleThe ADP-ATP Cycle

ATP-ATP-asease

ATP ATP SynthetaSynthetasese

When is ATP Made in When is ATP Made in the Body?the Body?

During a During a Process Process called called Cellular Cellular RespirationRespiration that takes that takes place in both place in both Plants & Plants & AnimalsAnimals

Cellular RespirationCellular Respiration Includes pathways that Includes pathways that

require oxygenrequire oxygen Glucose is Glucose is oxidizedoxidized and O and O22 is is

reducedreduced Glucose breakdown is Glucose breakdown is

therefore an therefore an oxidation-oxidation-reductionreduction reaction reaction

Breakdown of one glucose Breakdown of one glucose results in results in 36 to 38 ATP36 to 38 ATP molecules molecules

Overall Equation for Overall Equation for Cellular RespirationCellular Respiration

6CO6CO22 + 6H + 6H220 + e0 + e-- + 36- + 36-

3838ATP’sATP’s

CC66HH1212OO6 6 + 6O+ 6O22

YIELDSYIELDS

What Carries the What Carries the Electrons?Electrons?

NADNAD++ (nicotinadenine (nicotinadenine dinucleotide) acts dinucleotide) acts as the energy as the energy carriercarrier

NADNAD++ is a is a coenzymecoenzyme

It’s It’s ReducedReduced to to NADHNADH when it when it picks up two picks up two electrons and one electrons and one hydrogen ionhydrogen ion

Are There Any Other Are There Any Other Electron Carriers?Electron Carriers?

YES! Another YES! Another Coenzyme!Coenzyme!

FAD+FAD+ (Flavin (Flavin adenine adenine dinucleotide)dinucleotide)

ReducedReduced to to FADHFADH22

Other Cellular Other Cellular Respiration FactsRespiration Facts

Metabolic PathwayMetabolic Pathway that breaks that breaks down carbohydratesdown carbohydrates

Process is Process is ExergonicExergonic as High- as High-energy Glucose is broken into energy Glucose is broken into COCO22 and H and H22OO

Process is also Process is also CatabolicCatabolic because larger Glucose breaks because larger Glucose breaks into smaller moleculesinto smaller molecules

What are the Stages of What are the Stages of Cellular Respiration?Cellular Respiration?

GlycolysisGlycolysisThe Krebs CycleThe Krebs CycleThe Electron Transport ChainThe Electron Transport Chain

Where Does Cellular Where Does Cellular Respiration Take Place?Respiration Take Place?

It actually It actually takes place takes place in two parts in two parts of the cell:of the cell:

Glycolysis Glycolysis occurs in the occurs in the CytoplasmCytoplasmKrebs Cycle & Krebs Cycle & ETC TakeETC Take place place in the in the MitochondriaMitochondria

Review of Mitochondria Review of Mitochondria StructureStructure

SmoothSmooth outer outer MembraneMembrane

FoldedFolded inner inner membranemembrane

Folds called Folds called CristaeCristae

Space inside Space inside cristae called cristae called the the MatrixMatrix

Diagram of the Diagram of the ProcessProcess

Occurs in

Cytoplasm Occurs

in Matrix

Occurs across Cristae

Glycolysis Glycolysis SummarySummary

Takes place in the Takes place in the CytoplasmCytoplasm

Anaerobic Anaerobic (Doesn’t Use (Doesn’t Use Oxygen)Oxygen)Requires input of Requires input of 2 ATP2 ATP

Glucose split into two Glucose split into two molecules of molecules of PyruvatePyruvate or or Pyruvic AcidPyruvic Acid

Glycolysis Glycolysis SummarySummary

• Also produces Also produces 2 NADH and 4 2 NADH and 4 ATPATP

• Pyruvate is oxidized to Pyruvate is oxidized to Acetyl Acetyl CoACoA and and COCO22 is removed is removed

GlycolysiGlycolysis s

DiagramDiagram

FermentationFermentation

Occurs when OOccurs when O22 NOT present NOT present (anaerobic)(anaerobic)

Called Called Lactic AcidLactic Acid fermentation in muscle cells fermentation in muscle cells (makes (makes muscles tiredmuscles tired))

Called Called Alcoholic fermentationAlcoholic fermentation in yeast (produces in yeast (produces ethanolethanol))

Nets only Nets only 2 ATP2 ATP

A Little Krebs Cycle A Little Krebs Cycle HistoryHistory

Discovered by Discovered by Hans KrebsHans Krebs in in 19371937

He received the He received the Nobel PrizeNobel Prize in in physiology or physiology or medicine in medicine in 1953 for his 1953 for his discoverydiscovery

Forced to leave Forced to leave Germany prior Germany prior to WWII because to WWII because he was Jewishhe was Jewish

Krebs Cycle Krebs Cycle SummarySummary

Requires Oxygen (Aerobic)Requires Oxygen (Aerobic)Cyclical series of oxidation Cyclical series of oxidation

reactions that give off reactions that give off COCO22 and and produce produce one ATP per cycleone ATP per cycle

Turns Turns twicetwice per glucose molecule per glucose moleculeProduces Produces two ATPtwo ATP Takes place in Takes place in matrix of matrix of

mitochondriamitochondria

Krebs Cycle Krebs Cycle SummarySummary

Each turn of the Krebs Cycle Each turn of the Krebs Cycle also produces also produces 3NADH, 3NADH, 1FADH1FADH22, and 2CO, and 2CO22

Therefore, For Therefore, For each Glucoseeach Glucose molecule, the Krebs Cycle molecule, the Krebs Cycle produces produces 6NADH, 2FADH6NADH, 2FADH22, , 4CO4CO22, and 2ATP, and 2ATP

Krebs CycleKrebs Cycle

ATP

NETS: 3NADH, 1ATP, 1FADHNETS: 3NADH, 1ATP, 1FADH22, & 2CO, & 2CO22

Electron Transport Chain Electron Transport Chain SummarySummary

34 ATP34 ATP Produced ProducedHH22OO Produced ProducedOccurs Across Occurs Across Inner Inner

Mitochondrial membraneMitochondrial membraneUses coenzymes Uses coenzymes NAD+ and NAD+ and

FAD+ to accept e- from glucoseFAD+ to accept e- from glucoseNADH = 3 ATP’sNADH = 3 ATP’sFADHFADH22 = 2 ATP’s = 2 ATP’s

Electron Transport Chain AElectron Transport Chain Animationnimation