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Transcript of Adeel Rehan
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Baseline susceptibility and stability of insecticide
resistance in Spodoptera litura(F.)
Adeel Rehan, Mushtaq A. Saleem, Sarfraz A. Shad, Ghulam M. Cheema andUmair Faheem
University College of Agriculture, B. Z. University, Multan
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Introduction
Polyphagous defoliating insect pest found in Asia andOceania
Important crops which are attacked in Pakistan arecotton, berseem, maize, tobacco, groundnut, summer
legumes, and vegetables such as cucurbits, brinjal,potato, sweet potato, and brassica
May cause 26100% yield loss in the field
High resistance against conventional insecticides
Low to medium resistance against new chemistryinsecticides
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Life stages ofSpodoptera litura
Larva Eggs
PupaeMoths
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Main Objectives
To generate the baseline susceptibility data ofS. lituraagainst four different insecticides i.e. spinosad, emamectinbenzoate, imidacloprid and profenofos
To observe the susceptibility of succeeding generations ofS. liturato different insecticides under laboratoryconditions
To check the stability of insecticide resistance in S. litura
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Materials and Methods
Larvae ofS. liturawere collected from dunyapur
The larvae were reared on improved artificial diet at27C2 temperature and 70-80% humidity
Toxicological studies Insecticides
Spinosademamectin benzoateprofenofosimidacloprid
Bioassay methodDiet incorporation method
Data analysis
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7.77
4.57 4.72
3.95
2.53
1.66
1.140.79
0
1
2
34
5
6
7
8
910
LC50v
alue(ppm)
1 4 5 7 8 9 10 11Generations
Toxicity of spinosad against Spodoptera lituraunder laboratoryconditions
Results
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3.85
1.371.14 1.19 1.13
0.70.4 0.33
0
1
2
3
4
5
6
LC50
va
lue(ppm)
1 4 5 7 8 9 10 11
Generations
Toxicity of emamectin benzoate against Spodoptera lituraunderlaboratory conditions
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258.75
136.5
52.07 49.840.71
24.6219.61 18.71
0
50
100
150
200
2503
00
LC50value(ppm)
1 4 5 7 8 9 10 11Generations
Toxicity of profenofos against Spodoptera lituraunder laboratoryconditions
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686.5
359.3
314.7
134.6 131.896.1
75.14 73.5
0
10020030040
0500600700800
LC50
value(ppm)
1 4 5 7 8 9 10 11
Generations
Toxicity of imidacloprid against Spodoptera lituraunder laboratoryconditions
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Insecticides Susceptibility Index Resistance Decline
Initial LC50
(ppm)
Final LC50
(ppm)
RF R G
imidacloprid 686.5 73.5 9.34 -0.088 11.36
spinosad 7.77 0.79 9.83 -0.090 11.11
emamectin
benzoate 3.85 0.33 11.7 -0.096 10.41
profenofos 258.75 18.71 13.8 -0.104 9.61
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Conclusion
This baseline susceptibility data can be used for futureinsecticide monitoring ofS. litura
S. liturahas resistance against non-target insecticide
(imidacloprid) that may cause cross resistance problems.
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