Acupuncture and Natural Medicine 05–06/2015casopis.akupunktura.sk/Akupunktura_a_naturalna... ·...

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English MEDICAL SOCIETY OF NATURAL MEDICINE: REVIEWED SCIENTIFIC JOURNAL number 5–6, volume 2015

Transcript of Acupuncture and Natural Medicine 05–06/2015casopis.akupunktura.sk/Akupunktura_a_naturalna... ·...

English

MEDICAL SOCIETY OFNATURAL MEDICINE:

REVIEWED SCIENTIFIC JOURNAL

ACUPUNCTUREand

NATURAL MEDICINE

number 5–6, volume 2015

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Acupuncture and Natural Medicine 5–6/2015 3

Table of Contents

Table of Contents

Horizontal and vertical level of the pyramid model – selected relations and possible connections ....................................................... 7Gustáv Solár, M. D., PhD., Ing. Juraj Solár, Zlatica Solárová, M. P., PhD.

The Time Curvature in Acupuncture, Application in Clinic ............ 16Teodor Mochnáč, M. D., PhD.Experimental and Clinical Concept Essentials of Alloplant Regenerative Medicine ........................................................................ 22Prof. Ernst Rifgatovich Muldashev, M. D., DrSc., Radik Fayazovich Galiyakhmetov, M. D., CSc.Values of Intraocular Pressure and Acupuncture – Pilot Study ....... 32Magdaléna Miklósová, M. D.

Thermal Effects in Electro Acupuncture ............................................ 42Assoc. Prof., Dipl. Ing. Branislav Hučko, PhD., Ing. František Horvát, Ing. Tomáš Kováč, Ing. Lukáš Šoltés, Ing. Michal Čekan, PhD.Slope Movements as a Geofactors of the Environment and their Impact on Human ................................................................................ 49Prof. Dr. František Baliak, PhD., Mgr. Martin Brček, PhD.Fluorine and Health Problems in Žiarska kotlina Basin (Slovakia) . 57Assoc. Prof. Dr. Miloslav Khun, CSc.Human, Animal and Therapy ............................................................. 63Dr. Denisa Šoltésová, PhD., Mgr. Michaela Skyba, PhD.

Insights from the XIX Congress of Natural Medicine, Nitra, 16th–18th of October 2015.................................................................................... 78Teodor Rosinský, M. D., CSc.

Editorial Policy .................................................................................... 83Instructions for Authors ...................................................................... 84

Acupuncture Little Different

Clinic and Research

Interdisciplinary Insights

Reports from Professional Events

Informations

Editorial OfficeEditor-in-ChiefSoňa Sázelová, M. D.

Associate Editor Responsible for Foreign AffairsEva Baumann, M. D.

Associate Editor Responsible for Interdisciplinary StudiesMgr. Zuzana Šimová, PhD.

Editorial BoardSoňa Sázelová, M. D.Eva Baumann, M. D.Martina Lehocká, M. D.Magdaléna Miklósová, M. D.Katarína Loncková, M. D.Daniela Hurajová, M. D.PhDr. Valentín Dikarev

Technical Editor and Graphic DesignIng. Juraj Solár

Holder of the License to PublishThe First Clinic of Acupuncture and Natural Medicine of G. Solar, ltd.Executive Director: Zlatica Solárová, M. P., PhD.Ivanská cesta č. 23821 04 Bratislava, Slovak RepublicE-mail: [email protected]: www.naturalnamedicina.com

Acupuncture and Natural Medicine 5–6/2015 4

Editorial

Editorial

Dear readers,

It is a pleasure for me to write the introduction to the last issue of this journal at the end

of the year 2015. This time we decided to prepare a double issue for you. I believe you will enjoy the enhanced number of inspiring articles.

The authors Gustáv Solár M. D., PhD, Ing. Juraj Solár and Zlatica Solárová, M. P., PhD, present the study from the field of acupuncture theory. They continue in analysis of the unique pyramid model of acupuncture system and clarify the mutual connection of meridians in a horizontal and vertical plane of the proposed model. Teodor Mochnáč, M. D., PhD. also continues in his pre­vious contributions. This time he describes the use of his proposed model (Teo  Mo hexagram sequence) in a clinical practice.

The  team of authors led by Assoc. Prof. Ing. Branislav Hučko, PhD. from the Institute of Applied Mechanics and Mechatronics of STU enrich this issue with their pilot study on the thermal effect of electroacupuncture. The authors investigated the thermal effect of acupuncture using thermomechanical analysis. They propose the options how to identify the impact of the temperature effect of electroacupuncture treatment.

The  next two studies are from the field of ophthalmology. The first study is from the Russian authors Prof. Ernst  R. Muldashev, M. D., PhD. and  Prof. Radik  F. Galiyakhmetov, M. D., PhD. (the  Russian Centre of Eye and Plastic Surgery, Ufa). In this study they compared two methods of Alloplant biomaterial application in the treatment of corneal lesions on animal model. The second study is from Magdaléna Miklósová, M. D.

The  author evaluates the effect of acupuncture treatment on the intraocular pressure. At  the same time she presents an original application of acupuncture pyramid model in the anatomy and physiology of the eye.

The  contribution from the field of geology by Assoc. Prof. Dr. František Baliak, PhD. and Mgr.  Martin Brček, PhD. (The  Faculty of Civil Engineering, STU) explains the issue of geodynamic phenomena, which significantly affect the human environment. It  is mainly focused on the issue of slope movements, their positive and negative effects on human’s life and work as geopotential and geo­barriers, respectively.

The  contribution of Assoc. Prof. Dr. Miloslav Khun, PhD. to  this issue of the journal is in the monitoring of fluorine contamination in Žiar basin, which was part of the emission from an aluminium plant in Žiar nad Hronom. The  expected relationship between the con­centration of fluorine in the environment and increased incidence of endocrine and cancer diseases and birth defects are documented by health data of child population in the area.

Dr.  Denisa Šoltésová, PhD. and  Mgr. Michaela Skyba, PhD. highlighted in their article the positive impact of canine therapy for a mental and physical condition of  man. The  need for further intensive scientific research in the field of human­animal interactions is also emphasized.

In  conclusion, Teodor Rosinský, M. D., PhD. reports on the scientific program of  XIX. Medical Congress of Natural Medicine with interdisciplinary participation, held in Nitra,

Acupuncture and Natural Medicine 5–6/2015 5

Editorial

Scientific Council

Prof. Dr. František Baliak, PhD.STU Bratislava, Civil Engineering, Department of Geotechnics

Doc. MUDr. Vladimír Bella, PhD.chairman of SEKCAMA (Senology Society)Chief of Clinic of Mamology department, St. Elisabeth Oncology instituteHeydukova 10, 821 08 Bratislava

Dr. Katarína Bíliková, PhD.Dpt. of Molecular Apidology, detached branch of Institute of Forest Ecology, Bratislava

Associate Prof. Ing. Ján Brindza, CSc.Slovak University of AgricultureInstitute of Biodiversity Conservation and Biosecurity

Associate Prof. Marek Čambal, M. D., PhD.First Department of Surgery, Faculty of Medicine, Comenius University, Bratislava

Prof. Dušan Dobrota, M. D., CSc.Department of Medical Biochemistry, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava

Juraj Gajdoš, M. D.General Practitioners for Adults and Acupuncturist, N.z.z. Trnava

Associate Prof. RNDr. Vojtech Gajdoš, CSc.Geophysicist, Bratislava

Associate Prof., Dipl. Ing. Branislav Hučko, PhD.Dean of FME STU in BratislavaNám. Slobody 17 , 812 31 Bratislava, Slovak republic

Jean-Marc Kespi, M. D.Honorary President of the French Association of Acupuncture (AFA)

Associate Prof. Dr. Miloslav Khun, CSc.UK Bratislava, Faculty of Natural Sciences, Department of Geochemistry

Dr. Pil-Gun Kim, K.M.D., PhD,Korean Medicine Association, Soulpresident

Prof. Ing. Ján Košturiak, PhD.Managing Director, IPA Slovakia, Professor Tomas Bata University Zlin

Ing. Eva Kutejová, CSc.Dpt. of Biochemistry and Structural Biology, Institute of Molecular Biology, SAV, Bratislava

Anna Loskotová, M. D.Chief of Clinic of Physiotherapy – SALVE Centre, Vysoke Myto, Czech republicMASARYK University in Brno, Clinic of Burns and Reconstructive Surgery, University hospital in Bohunice, Czech republic

Folker Meissner, M. D.Chairman of the German Academy of Energy, Medicine and Bioenergetics (DAEMBE), Clinic for Holistic Medicine Dr. Folker Meissner

from 16th to 18th October 2015. His report brings a complete and concise overview of the presented lectures to the readers.

The  main priority of the editorial board is to enhance the quality and proficiency of the journal so we decided to change its periodicity. The journal will be published quarterly from the next issue.

I  hope you spent a peaceful and beautiful Christmas time. I  wish you a happy and successful new year as well as a lot of motivation and creativity in your work. We look forward to your interesting manuscripts.

Sincerely yours,Mgr. Zuzana Šimová, PhD.

Acupuncture and Natural Medicine 5–6/2015 6

Scientific Council

Teodor Mochnáč, M. D., PhD.Akupunktum, Center of acupuncture and diabetic foot, Nitra

Prof. Marián Mokáň, M. D., DrSc., FRCP EdinDepartment of Internal Medicine I., Jessenius Faculty of Medicine Comenius University and University Hospital Martin

Prof. Ernst Rifgatovič Muldašev, M. D., DrSc.Russian Eye and Plastic Surgery Center, Ufa, Russian FederationDirector

Prof. Radik Talgatovič Nigmatulin, M. D.Russian Eye and Plastic Surgery Center, Ufa, Russian FederationDeputy Director for Science and Research

Prof. Chi-Chung PengAssociate Prof. & Chairman, National Formosa University, Department of Biotechnology, Huwei, Yunlin, Taiwan

Prof. Dr. Phys.-Math. Sergej Petoukhov, PhD.Laureate of the State prize of the USSR, academicianChief of the Laboratory of biomechanical systems of the Mechanical Engineering Research Institute of the Russian Academy of Sciences, Moscow, Russian Federation

Associate Prof. Jana Slobodníková, M. D., CSc. h. Prof.Faculty of Health, KLVM, Alexander Dubcek University of Trencin

Gustáv Solár, M. D., PhD.The First Clinic of Acupuncture and Natural Medicine of G.Solar s. r. o.,professional supervisor

Zlatica Solárová, M. P., PhD.The First Clinic of Acupuncture and Natural Medicine of G.Solar s. r. o.,executive director

Denisa Šoltésová, M. P., PhD.Associate Director of Institute of Educology and Social WorkInstitute of Educology and Social WorkFaculty of Arts, University of Presov in Presov

Prof. Miron Šramka, M. D., DrSc.St. Elisabeth Cancer Institute, Bratislava, Department of stereotactic radiosurgery, St. Ellisabeth University of Health and Social Sciences, Bratislava

Dr. Stângaciu StefanPresident of Apitherapy Consulting & Trading International Ltd.President of the Romanian and German Apitherapy Societies.Secretary General of the International Federation of Apitherapy

Prof. Park Wan SuThe Chief Vice President of AKOM (the Association of Korean Medicine)Professor, Dept. of Pathology, College of Korean Medicine, Gachon University, Republic of Korea

Kikuji Yamaguchi, PhD.Company Chairman & CEO, Yunnan Agricultural University, Kyobashi Chuo-ku, Tokyo

Jeong Yeonil M. D.Korean Medicine Association, SeoulDirector for Foreign Affairs

Acupuncture and Natural Medicine 5–6/2015 7

Acupuncture Little DifferentHorizontal and vertical level of the pyramid model – selected relations and possible connections

Gustáv Solár, M. D., PhD., Ing. Juraj Solár, Zlatica Solárová, M. P., PhD.

Horizontal and vertical level of the pyramid model – selected relations and possible connectionsGustáv Solár, M. D., PhD., Ing. Juraj Solár, Zlatica Solárová, M. P., PhD.

SummaryThere are many mutual interactions and relations in the complex dynamic pyramid model of

acupuncture (hereinafter “pyramid model”) both on the horizontal and the vertical level. In  the present study the authors analyse relations on the vertical level of the pyramid model. For  the purpose of defining the essence of Yang and Yin as dynamic states of the system, relations on the horizontal level were analysed  too. The  vertical level of the model consists of meridians of the large intestine (IC), stomach (VE), liver (HE), urinary bladder (VU), and  the heart meridian (CO). The  meridian of urinary bladder (VU) has a special position as it is a part of both the vertical and horizontal level. A complex analysis of mutual as well as internal relations on both levels requires further and more detailed interdisciplinary research. Namely, it is necessary to analyse medical, physical, mathematical and other characteristics of these interactions. Their universal character surpasses the medical level represented by acupuncture. The study confirmed the assumed different characteristics of Yang and Yin states of the system and their mutual connections. The complex mutual connections of meridians on both horizontal and vertical levels were confirmed too.

Key wordsacupuncture system, complex dynamic pyramid model in acupuncture, triplets, simple meridians, complex meridians, yang rotations, yin rotations

IntroductionThe pyramid model in acupuncture or the pyramid arrangement of the acupuncture system has two levels  – horizontal and vertical. The  horizontal level has already been analysed broadly.[7,  4] It  consists of eight meridians arranged in four octograms. These are arrangements according to Fu­Shi, Wen, S1  and  S2. They alternate and pass into each other through the rotation of the yang and yin concatenations.[4] The arrangement of octograms according to Fu­Shi (the  essential basis) is static, the remaining three arrangements (Wen, S1 and S2) are dynamic.[4] Characteristics of this rotational movement differ in the same way as the four positions in the four octograms in case of every meridian (tab. 1).

The vertical level of the pyramid model consists of meridians of the large intestine (IC), stomach (VE), liver (HE), urinary bladder (VU) and  the heart meridian (CO). The  meridian of urinary bladder (VU) has a special position as it belongs to both the vertical and horizontal level (tab. 1).[5]

The  nature of the acupuncture system implies that all meridians must be interconnected and functionally matching to ensure its optimal function. As  we have previously mentioned, meridians are connected through triplets and

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Gustáv Solár, M. D., PhD., Ing. Juraj Solár, Zlatica Solárová, M. P., PhD.

the triplet sequences may differ.[5] That is one of the mechanisms that ensure a variability of interconnections in the acupuncture system as a whole. The next mechanism is the interconnection of meridians on the horizontal and vertical level, which should be complex. The analysis of mechanisms of such a connection is the subject of this study.

Material and methodsIn  the octograms of the horizontal level of the pyramid model of the acupuncture system arranged according to Fu­Shi, Wen, S1 and S2 the first four positions of each meridian (tab. 1) were assessed. The rotations were assessed clockwise; a  possible rotation in the opposite direction would not change the character of rotations of the individual meridians (it would probably result in a mirror reflection).

Based on the static arrangement according to Fu­Shi, if we shift the yang and yin concatenations into the next position (the yang concatenation to the left and the yin to the right), we will get the arrangement according to  Wen. Analogically, a  further shift of concatenations into the next position will result in the S1  arrangement and one more shift will result in the S2 arrangement. The  following shift from the S2  arrangement will result in Fu­Shi arrangement and the cycle repeats. That means that there are altogether four positions. We  illustrated the positions graphically, which resulted in rotational curves typical for individual meridians on the horizontal level (fig. 1).

Relations among meridians in the circadian rhythm (the  midnight­noon rule) were used to pair meridians on the horizontal and vertical level. The  circadian rhythm expresses both the functional and regulative characteristics and relations in the yang and yin meridians.

We  assessed relations on all four axes of the octogram (vertical, horizontal and two diagonal axes) in all four variants of the octograms.For  the purpose of this study we used triplets according to yang and yin concatenation.

ResultsThe  paired meridians based on the circadian rhythm (the  midnight­noon rule) between the horizontal and vertical level of the pyramid model are as follows:

– the  heart meridian (CO)  – the  gall bladder meridian (VF)

– the  liver meridian (HE) – the small intestine meridian (IT)

– the stomach meridian (VE) – the pericardium meridian (PE)

– the large intestine meridian (IC) – the kidney meridian (RE)

The urinary bladder meridian (VU) has a special position as it belongs to both the vertical and horizontal level and is paired with the lungs meridian (PU). The VU­PU axis is the most stable axis of the horizontal level because it is the only one present in all four variants of the octogram. In each variant of the octogram it forms one of the four basic axes (horizontal – Fu­Shi, vertical – Wen and two diagonal ones – S1 and S2). In this way the VU­PU axis forms all the basic axes of the octogram on the horizontal level in the full cycle of the octogram shift (tab. 1).

The  urinary bladder meridian (VU) lies on the vertical level between the second and the third under the heart meridian (CO) on the peak and above the liver meridian (HE) in the area of the so­called “pharaoh chamber” pyramid. On  the vertical level a representative of the WATER element appears and thus all 5  elements are represented (tab.  1). If  the bladder meridian (VU) meridian represents the TIME function (the  information flow and the verticalization)

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Gustáv Solár, M. D., PhD., Ing. Juraj Solár, Zlatica Solárová, M. P., PhD.

and the lungs meridian (PU) meridian the SPACE function (the  state of the system and external signs), then the VU –PU axis may be considered a stable space­time axis of the system rotation. This arrangement connects meridians of the yang and yin concatenation on the horizontal and vertical level (tab. 2).

Table  1 shows the rotation of meridians in four arrangements according to Fu­Shi, Wen, S1 and F2.

Tab. 1 The order of meridians on the horizontal level (pakua universal), circular arrangement from a to h according to Fu­Shi1 Wen2 S13 and S24 arrangement and the vertical axis of meridians and elements CO (Fire), VU (Water), HE (Wood), VE (Earth), and IC (Metal).

Horizontal levelIT1h VF2h LP1a PU2a VF1b RE2b CO FireRE4h VU3h PE4a TC3a VU4b IT3b VU WaterPU1g TC2g VU1c IT2c HE WoodLP4g PE3g VF4c RE3c VE EarthTC1f PE2f RE1e VU2e PE1d LP2d IC MetalPU4f LP3f IT4e VF3e TC4d PU3d

Legend: Rotation of the VU – PU axis on the horizontal level. PU2a – VU2e – vertical axis, VU3h – PU3d, VU4b – PU4f diagonal axis, PU1g  –  VU1c  – horizontal axis, arrangement in the octogram from a to h

Tab. 2 Space­time on the horizontal and vertical level of the pyramid model

Horizontal level Vertical level

Time3h Space2a Time4b Time / Space

Space1g Time1c

Space4f Time2e Space3d

Legend: horizontal level Fu­Shi1  – horizontal axis of the space­time, Wen2  – vertical axis of the space­time, S13, S24 – diagonal axes of the space­time, vertical level  – space­time on the boundary of two thirds, arrangement in the octogram from a to h

a b b c d a

d c a d c b

Fig. 1 Rotation of the yin concatenations a–b–c–d and a clockwise and a counterclockwise phase shift

The  rotation of the yin concatenations on the horizontal level means a counterclockwise and a clockwise rotation of meridians and a shift into the neighbouring position (from  4176 to 1764, etc. (fig. 1)).

The rotation of the yang concatenations on the horizontal level is a rotation of meridians and a system of crossing.

a b b d c a

d c c a b d

Legend: Pakua sequence FU­SHI1a…h, Wen2a…h, S13a…h, S24a…h.

Meridians of the horizontal axis from a to h and the vertical axis CO, VU, HE, VE and IC:

– blue field – yin concatenation, – red field – yang concatenation.

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Acupuncture Little DifferentHorizontal and vertical level of the pyramid model – selected relations and possible connections

Gustáv Solár, M. D., PhD., Ing. Juraj Solár, Zlatica Solárová, M. P., PhD.

Tab. 3 The order of meridians, horizontal level (pakua universal) according to Fu­Shi, Wen, S1, S2 and the vertical axis of CO, VU, HE, VE and IC meridians, vertical and horizontal axis.

3 8 4 1 8 22 5 6 7 5 31 7 5 34 6 8 27 6 2 5 6 41 4 3 8 7 1

FU­SHI, Wen, S1, S2 octogram sequence:1 = lungs meridian (PU),2 = kidneys meridian (RE),3 = small intestine meridian (IT),4 = spleen, pancreas meridian (LP),5 = urinary bladder meridian (VU),6 = pericardium meridian (PE),7 = triple heater meridian (TC),8 = gall bladder meridian (VF)

Meridians of the horizontal level in the circular arrangement from a to h and the vertical axis from CO, VU, HE, VE and IC:

– vertical axis – horizontal axis

Tab. 4 Rotations of meridians of the vertical axis

M. Fu-Shi WEN S1 S2

CO VF – TC VF – LP VF – TC VF – LP

VU VU – PU VU – PU VU – PU VU – PU

HE IT – PE IT – TC IT – LP IT – PE

VE PE ← IT PE ← RE PE ← RE PE ← IT

IC RE – LP RE – PE RE – PE RE – TC

Tab.  4 shows how the meridians of the vertical axis CO, VU, HE, VE and IC are interconnected with all four axes of the horizontal level in one full cycle. That means that the yang and yin concatenations and both levels of the pyramid model in one rotational cycle – both the vertical and the horizontal are completely interconnected.

Each of the meridians of the horizontal level has a different characteristic rotational curve. With meridians of the yin concatenation it is always the right rotations (yin), where the individual curves of meridians are phase­shifted in relation to each other (tab. 3).

It  refers to high stability and a relatively small dynamics of the system, which is typical for the yin characteristics.

Tab. 5 Yin concatenation of meridians of spleen (LP), lungs (PU), triple heater (TC) and pericardium (PE) and a phase shift of meridians on the horizontal level

4 9 23 5 78 1 6

9

68

3

Meridian PE – yin concatenation

Yin rotation

4 9 23 5 78 1 6

9

68

3

Meridian TC – yin concatenation

Yin rotation

4 9 23 5 78 1 6

9

68

3

Meridian PU – yin concatenation

Yin rotation

4 9 23 5 78 1 6

9

68

3

Meridian LP – yin concatenation

Yin rotation

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Acupuncture Little DifferentHorizontal and vertical level of the pyramid model – selected relations and possible connections

Gustáv Solár, M. D., PhD., Ing. Juraj Solár, Zlatica Solárová, M. P., PhD.

IT1h VF2h LP1a PU2a VF1b RE2b

RE4h VU3h PE4a TC3a VU4b IT3b

PU1g TC2g VU1c IT2c

LP4g PE3g VF4c RE3c

TC1f PE2f RE1e VU2e PE1d LP2d

PU4f LP3f IT4e VF3e TC4d PU3d

Legend: yin concatenation LP, PU, TC, PE and a phase shift of meridians (LP1a  – LP2d  – LP3f  – LP4g), (PU1g  – PU2a  – PU3d  – PU4f), (TC1f  – TC2g  – TC3a  – TC4d), (PE1d – PE2f – PE3g – PE4a)

phase shift LP1a – PU1g – TC1f – PE1d

blue field – yin concatenation

Tab. 6 Yang concatenation of meridians: mirror reflection of urinary bladder (VU), gall bladder (VF), kidneys (RE) and small intestine (IT) on the horizontal level

IT1h VF2h LP1a PU2a VF1b RE2b

RE4h VU3h PE4a TC3a VU4b IT3b

PU1g TC2g VU1c IT2c

LP4g PE3g VF4c RE3c

TC1f PE2f RE1e VU2e PE1d LP2d

PU4f LP3f IT4e VF3e TC4d PU3d

Legend: yang concatenation of mirror reflection of meridians VU  –  VF  a RE  –  IT  and “tangling” of meridians RE  –  IT  in the following order:VU1c – VU2e – VU3h – VU4b

VF1b – VF2h – VF3e – VF4c

RE1e – RE2b – RE3c – RE4h

IT1h – IT2c – IT3b – IT4e

red field – yang concatenation

Different characteristics were found with meridians of the yang concatenation. These form two mirror pairs and the VU and VF meridians form typical rotational yang and yin curves, but only in the octogram in positions of the yang concatenation (tab. 6, fig. 2 and 3).

4 9 23 5 78 1 6

4

1

7

2

Meridian VU – yang concatenation

Yin rotation

Fig. 2 Meridian VU, yang concatenation

4 9 23 5 78 1 6

4

1

7

2

Meridian VF – yang concatenation

Yang rotation

Fig. 3 Meridian VF, yang concatenation

RE and IT meridians create a mirror pair, however, they do not form a smooth rotational curve, but a loop. Therefore, we named these quasi yang and quasi yin rotations. (tab. 6, fig. 4 and 5)

4 9 23 5 78 1 6

4

1

7

2

Meridian RE – yang concatenation

Quasi yang rotation

Fig. 4 Meridian RE, yang concatenation

Acupuncture and Natural Medicine 5–6/2015 12

Acupuncture Little DifferentHorizontal and vertical level of the pyramid model – selected relations and possible connections

Gustáv Solár, M. D., PhD., Ing. Juraj Solár, Zlatica Solárová, M. P., PhD.

4 9 23 5 78 1 6

4

1

7

2

Meridian IT – yang concatenation

Quasi yin rotation

Fig. 5 Meridian IT, yang concatenation

It is currently not possible to describe the rate and spacial arrangement without further research. The  given arrangement of curves of the yang

concatenation shows great dynamics (unlike the yin concatenation) as well as instability, which is typical for the yang.

Table 7 shows triplets of individual meridians on the horizontal level.

Tab. 7 Triplets of meridians on the horizontal level

Yang concatenation Yin concatenationVF RE IT PE LP PURE IT VU LP PU TCIT VU VF PU TC PE

VU VF RE TC PE LP

Tab. 8 Interactions of meridians on the vertical level

11 13 16 17 19 21 23 01 03 06 07 09 11

CO 4× [0] CO IT VU RE PE TC VF HE PU IC VE LPVU 2× [0] CO IT VU RE PE TC VF HE PU IC VE LPHE 7× [2] CO IT VU RE PE TC VF HE PU IC VE LPVE 6× [2] CO IT VU RE PE TC VF HE PU IC VE LPIC 6× [1] CO IT VU RE PE TC VF HE PU IC VE LP

2× 2× 1×! 2× 3× 3× 1× 2× 1×! 3× 2× 3×

basic pair auxiliary meridian subsidiary meridian vertical and also horizontal!

TC 3× [3] CO IT VU RE PE TC VF HE PU IC VE LPLP 3× [3] CO IT VU RE PE TC VF HE PU IC VE LPPU 1 [0] CO IT VU RE PE TC VF HE PU IC VE LP

2× 2× 1×! 2× 0× 2× 2× 2× 1×! 2× 0× 2×

Acupuncture and Natural Medicine 5–6/2015 13

Acupuncture Little DifferentHorizontal and vertical level of the pyramid model – selected relations and possible connections

Gustáv Solár, M. D., PhD., Ing. Juraj Solár, Zlatica Solárová, M. P., PhD.

CO (VF, TC, LP)

HE (IT, PE, TC, LP) VE (PE, IT, RE) IC (RE, LP, PE, TC)

VU (VU, PU)

Fig. 6 Possible vertical space variations

Meridians of the horizontal level are basic meridians that interact with each other while these interactions vary. Meridians of the vertical level do not have their own rotational characteristics but determine the relations with meridians of the horizontal level via the paired meridians of the circadian rhythm (tab.  3). They may be characterized as meridians of higher order that provide variable interactions with the horizontal level. Mainly, they influence the more complex functions and the verticalization of organism, i.e. its development. This is true for the somatic, psychoregulative and energy­informational level. A malfunction of meridians on the vertical level – provided another malfunction of a meridian paired with them occurs simultaneously  – must be considered a complex and a more serious malfunction than a simpler and isolated malfunction of a meridian on the horizontal level. Despite the current empirical experience we present this knowledge as theory resulting from the arrangement of the pyramid model.

DiscussionWe  have found no mention of the pyramid model in the available sources, with the exception of publications by the authors of this study.[4,  5,  6] Neither any information about the basic meridians or meridians of the higher order according to the pyramid model is available. The  pyramid model cannot be constructed without the S1  and S2  octograms according to Wen and Fu­Shi.[1] The  current models of the acupuncture system, mainly the arrangement of the pentagram (Wu­Sin theory) are too general. They do not represent the whole dynamics of relations and arrangements of the acupuncture system. In  no case does the pyramid model disproves this arrangement; on  the contrary, it broadens and completes it in a considerable way. Neither does it disprove other generally accepted rules (midnight­noon rule, circadian rhythm, arrangement of octograms according to  Wen,

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Acupuncture Little DifferentHorizontal and vertical level of the pyramid model – selected relations and possible connections

Gustáv Solár, M. D., PhD., Ing. Juraj Solár, Zlatica Solárová, M. P., PhD.

Fu­Shi, etc.). It just unifies them into one model and opens new possibilities for further complex study of the acupuncture system.

The  terms Yang and Yin are a big issue for the Wu­sin theory. The  attempts to point out analogies with these terms (e. g. sympaticus and parasympaticus, male and female principle, etc.) do not express their essence and meaning. As such they are practically unacceptable by other medical fields, although in acupuncture they are crucial for the description of a certain principle or state. The graphical representation of relations inside the pyramid model (on  the horizontal level) displays the essence of the terms yang and  yin. It  proves their basic characteristics in the dynamic and stable state as well as their basic difference on the one hand and interconnection and inseparability on the other hand. We named the graphical representation of the meridians’ dynamics “rotations” and “rotation steps”. In this sense the Yang and Yin characteristics are rotation­determined. Meridians of the Yin concatenation are laevorotary[8] and  mutually different in the phase shift in the Yin positions of the octograms. Meridians of the Yang concatenation form two laevorotary and two dextrorotary pairs in the Yang positions of the octograms. We  chose the dextrorotary rotation of octograms in the Fu­Shi, Wen, S1 and S2 sequence. We would get analogical differences in the opposite rotation.[8]

We  chose a dextrorotary rotation (clockwise) with respect to the common, geometrically displayed direction of rotation and time sequence of individual arrangements (Fu­Shi­Wen­S1­S2). What remains open is the question whether the system appears only in these four states as “superpositions” or also in the so­called “in­gaps” states, i.e. whether the movement of the system is jumping – “digital” or smooth – “analogue”.

One  cycle (complete rotation of octograms) interconnects both levels of the pyramid model and all meridians. Further explicitly interdisciplinary research is necessary since the spatial arrangement, the  rotation speed and perhaps also some other characteristics are yet to be described. On the other hand they provide space for mathematical modelling and some other (e. g. physical) possibilities for research. We may state with certainty that the Yang and Yin states have their own specific physical characteristics. The  Yin meridians show considerably greater spatial stability and harmony. The Yang meridians show significant spatial variability and dynamics.

The results of our study prove that each meridian has a different characteristics and each can have triplet sequences in space. What is yet to be researched are the four axes on the horizontal level connecting meridians of the Yin and Yang concatenation.[8]

Principles of the pyramid model of the acupuncture system surpass the boundaries of the acupuncture system and in fact they can be considered universally valid. Acupuncture is just a clinical application of considerably broader relations and connections.

Some influence of the morphic fields[2] on  the dynamics of these processes is to be expected. Empirically, we may confirm the clinical use of the pyramid model, which however requires deeper clinical research, including the possibilities of modelling the physiological and pathological states.

ConclusionThe  present study confirmed that inside the pyramid model of the acupuncture system all the horizontal and vertical relations are interconnected. All five meridians of the vertical axis of the pyramid model are interconnected

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Acupuncture Little DifferentHorizontal and vertical level of the pyramid model – selected relations and possible connections

Gustáv Solár, M. D., PhD., Ing. Juraj Solár, Zlatica Solárová, M. P., PhD.

with the four basic axes (vertical, horizontal and two diagonal) in one full cycle of complete rotation of four octograms in the Fu­Shi – Wen – S1 – S2 sequence. In this way both levels of the pyramid model – horizontal and vertical are also fully connected. Each meridian has different characteristics, and not only functional, but also physical, which is expressed by rotations. Each meridian can have triplet sequences in space. The  Yang meridians create two mirror pairs of laevorotary and dextrorotary rotations in the yang positions of the octograms and have considerable spatial variability and dynamics. The  Yin meridians have significantly greater spatial stability and harmony. The pyramid model does not disprove the simpler and generally accepted model of pentagram or any other rules; it only broadens and completes them in a significant way. It unifies the rules into one model and opens new possibilities for a complex study of the acupuncture system.

Gustáv Solár, M. D., PhD., Zlatica Solárová, M. P., PhD., Ing. Juraj SolárThe First Clinic of Acupuncture and Natural Medicine of G. Solar, ltd., Ivánska cesta 23, Bratislava, Slovak republicE-mail: [email protected]

References1. Kral, O.: I­ťing – kniha proměn. Maxima, Praha,

1996, s. 18–20, ISBN 80­901333­3­9.2. Sheldrake, R.: Tao přírody. Gardenia Publishers,

Bratislava, 1990, ISBN 80­85662­10­8.3. Solár, G.: Triplets, Concatenation, Bagua and

Patterns in Acupuncture. In Acupuncture and Natural Medicine, Bratislava, MSNM, 2014, 2, p. 11–12, ISSN 1339­4703.

4. Solár, G., Solárová, Z.: The Complex Dynamic Pyramid Model in Acupuncture. In Acupuncture and Natural Medicine, Bratislava, MSNM, 2014, 5, p. 8–9, ISSN 1339­4703.

5. Solár, G., Solárová, Z.: Vertical Relations of the Pyramid Model in Acupuncture. In Acupuncture and Natural Medicine, Bratislava, MSNM, 2015, 1, p. 6–10, ISSN 1339­4703.

6. Solár, G., Solárová, Z.: Pyramid Model in Acupuncture and Its Application in Practice. In Acupuncture and Natural Medicine, Bratislava, MSNM, 2015, 2, p. 6–14, ISSN 1339­4703.

7. Solár, G., Solárová, Z.: Horizontal Relations of the Pyramid Model in Acupuncture. In Acupuncture and Natural Medicine, Bratislava, MSNM, 2014, 6, p. 8–9, ISSN 1339­4703.

8. Solár, G., Solárová, Z., Solár, J.: Some Possible Physical Links of the Pyramid Model in Acupuncture. In Acupuncture and Natural Medicine, Bratislava, MSNM, 2015, 4, p. 9–10, ISSN 1339­4703.

Acupuncture and Natural Medicine 5–6/2015 16

Clinic and ResearchThe Time Curvature in Acupuncture, Application in Clinic

Teodor Mochnáč, M. D., PhD.

The Time Curvature in Acupuncture, Application in Clinic

Teodor Mochnáč, M. D., PhD.

SummaryIn  this article the author reports about clinical use of the proposed Teo Mo Hexagram Sequence model in acupuncture. This model connects the diagnostic and therapeutic model through the instrumental IDS­M examination. It also stresses the need for the involvement of a personal mind­set with a focus on the way how elements interact and on changes of all elements in the pentagram.The  applied therapy utilizes one antique point on the group of 31 polymorbid patients, 9 men and 22  women, who  were examined before the treatment and 7  days after treatment. Results confirm the justification of this therapy with a 90  % success rate in improving of the patient’s clinical status.

Key WordsThe Teo Mo Hexagram Sequence, the acupuncture treatment, IDS­M (Impedance Data System M)

IntroductionThe  contest of first two parts of the topic was to provide an explanation for the principle of hexagrams’ sequence creation and description of the possibilities how the sequence can be used in acupuncture practice.[4, 5] A  model has been described, on  a basis of which it is possible to use these relationships also as in connection with the objective instrumental examination. In  this part we provide results of the practical use of this model, which can be also an inspiration for further research of human processes.

Every action is accompanied by a change of the matter structure, change of time and change of the form in different dimensions. This study should prove it, too.

Our  basic assumption is that elements are hierarchic superior to all material dimensions. Therefore, we can change the structure of the mass by changing any of the elements’ characteristics e. g. the  time changes the structure of the mass, or  the change of the mass structure can change the time.

File of PatientsIn  this study we have included 31  polymorbid patients, 22  women and 9  men, in  whom we have performed 100 instrumental examination of IDS­M (Impedance Data System­M) and 100 TST DS­M (Tactile Solar’s Test Data System M) tests.

MethodologyThe  methodology is based on the basic Teo Mo Hexagram Sequences model, in  which the mathematical trigrams arrangement is reflected in hexagrams in the circadian biorhythm timeline of ancient points.[4] Instrumental examinations, such as IDS­M (impedance data system  M) and  TST DS­M (Tactile Solar’s Test Data Sys­tem  M), make up the possibility of creation of the hexagrams status based on gradients of the electrical skin conductivity or tactile sensitivity have incorporated into the methodology. They can be used to determine the current dynamic status (acupuncture diagnosis) of the patient. The goal of treatment is to minimize these gradients and to establish an approximately balanced state.

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Clinic and ResearchThe Time Curvature in Acupuncture, Application in Clinic

Teodor Mochnáč, M. D., PhD.

In  chosen group of patients mentioned above we examined the current status by the IDS­M methodology in the regime of biorhythm points (RmEAB), in  the regime of complex meridians (extraordinary vessels) points (Rm  KM) and  in the complex regime (RmC). This way we have got a quantitative picture of each element in form of the weighted diagram.

On  the basis of this diagram we can determine qualitative characteristics of interactions between elements: generalized super­algorithm, paired super­algorithm, quantitative and qualitative algorithm, whereby the quantitative and qualitative algorithms can be further classified as either hyper­compositional or de­compositional, whereby both can be further either pure or turbulent.[1, 2]

The  quantitative picture in the form of the weighted diagram we obtain through the tactile TST DS­M examination, which is based on the examination of the gradients of seven microsystems i. e. RTF (Rotary microsystem of the face), RYIN (Rotary yin microsystem), RYANG (Rotary yang microsystem), RMMdx (Rotary breast microsystem right and left), RCES (Rotary microsystem of complex meridians), RUTMT (Rotary universal tend­muscular test) and  the result of IDS­M (Impedance data system M).On the basis of the complete TST DS­M picture we determine the current state of the body in the form of hexagram view. We  described the hypothesis, methodology of the examination of energy­informational changes in previous articles.[4,  7,  8] Gradients in weighted diagram

of TST DS­M create the intersection in TeoMo hexagram sequence of antique points. We  use it in treatment.[4] However, we  consider more important to determine the energy­informational state, the  interaction between elements and our energy­informational aim rather than the acupuncture point. Since the treatment is realized by the therapist and not by the acupuncture point. We  apply the acupuncture needle to the corresponding antique point, which corresponds to this condition. The result of the treatment has been controlled through the balanced tactile test of microsystems. After 7  days, we  made a repeated examination of IDS­M and TST DS­M. On the basis of two instrumental examinations at the same time we are able to determine changes in the quantitative picture of elements or to watch the nature of the interaction, which was used during treatment.

Definitions of TermsThe  relationship of the control, birth, anti­control, anti­birth, generalized super­algorithm and paired super­algorithm, which can be hyper­compositional and de­compositional pure and turbulent, have been defined already in the previous article.[1, 2] New terms resulting from the IDS­M’s need  i. e. quantitative and qualitative change, quantitative and qualitative algorithm, we define as follows:1. The  quantitative change is the change

where one or two elements do not fulfil the quantitative characteristic of the individual range of the norm. The failure of the dynamics is yang or it creates a yang gradient.

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Clinic and ResearchThe Time Curvature in Acupuncture, Application in Clinic

Teodor Mochnáč, M. D., PhD.

Fig. 1 Quantitative change of one element (FIRE). The failure of the dynamics is yang

Legend: red circle  = FIRE, yellow circle  = EARTH, blue circle = METAL, black circle  = WATER, green circle  = WOOD

Fig. 2 Quantitative change of two elements (EARTH and METAL)

Legend: red circle  = FIRE, yellow circle  = EARTH, blue circle = METAL, black circle  = WATER, green circle  = WOOD

2. The qualitative change is a change when two elements in the pentagram, being control relationship with each other, do not fulfil the quantitative characteristic of the individual range of the norm. The  disorder of the dynamics is expressed in the yin gradient.

Fig. 3 The qualitative change (FIRE and WATER)

Legend: red circle  = FIRE, yellow circle  = EARTH, blue circle = METAL, black circle  = WATER, green circle  = WOOD

3. The  quantitative algorithm is the name for the pathological process of three successive elements in pentagram in case the dynamic’s failure in yang to yin is 2 : 1.

Fig. 4 The quantitative algorithm (Decompositional turbulent WOOD)

Legend: red circle  = FIRE, yellow circle  = EARTH, blue circle = METAL, black circle  = WATER, green circle  = WOOD

4. The  qualitative algorithm is the name for the pathological process of two consecutive elements in the pentagram where the third element is in control relation to them. This algorithm always starts through the yang

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Clinic and ResearchThe Time Curvature in Acupuncture, Application in Clinic

Teodor Mochnáč, M. D., PhD.

relationship of the elements (the relationship of the birth) in a clockwise direction (a dynamic’s failure of yang to yin is in ratio 1 : 2).

Fig. 5 The qualitative algorithm (hyper­compositional turbulent EARTH)

Legend: red circle  = FIRE, yellow circle  = EARTH, blue circle = METAL, black circle  = WATER, green circle  = WOOD

5. The equilibrium state is an ideal state of balance, which occurs when the quantitative values of all pentagrams’ elements of the weighted diagram are in the zone of the individual norm range. Such condition is achieved in the clinical practice rarely. The reason for it is that the physical state of a person is dynamic and changes constantly. The  measurement of the electrical skin conductivity is only an expression of the current status in time.

Fig. 6 The equilibrium state of all elements (the quantitative state of the each element is in the zone of individual norm range – the width of the green ring)

Legend: red circle  = FIRE, yellow circle  = EARTH, blue circle = METAL, black circle  = WATER, green circle  = WOOD

6. The control relationship between all elements is the goal of each therapy i. e. the quantitative change of all elements in the weighted IDS­M diagram. Therefore, we define:Control relationship of the third (III) level is a characteristic that is applied in the evaluation of the therapy focusing on affecting all elements in the pentagram. It means that after therapy with a certain element’s characteristic it comes to an expected change at least in 3 elements of the pentagram.Control relationship of the fourth (IV) level is a characteristic that is applied in the evaluation of therapy focusing on affecting all elements in the pentagram. It means that after therapy with a certain element’s characteristic it comes to an expected change in at least 4 elements of the pentagram.

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Clinic and ResearchThe Time Curvature in Acupuncture, Application in Clinic

Teodor Mochnáč, M. D., PhD.

The Aim of the Study1. To  justify the competence of the model Teo

Mo Hexagram Sequence.2. To  confirm the clinical effectiveness of the

new model.3. To observe the quantitative changes in IDS­M

after application of one needle.

Results

men women

Graph 1 The number of men and women in the group

Age distribution in the group (number)

21–30 21–30 21–30 21–30 21–30

Graph 2 Age distribution in the group

Serie 1 Serie 2

112.5 113 %

90.0 90 %

67.5 68 %

45.0 45 %

22.5 23 %

0.0 0 %TST balanced,

89 %TST partially

balanced, 3 %TST nonbalanced,

8 %

Graph 3 The effectiveness of the therapy [M1]

Superalgorithm generalized

Superalgorithm pair

Decompositional turbulent algorithm

Decompositional clean algorithm

Hypercompositional turbulent algorithm

Hypercompositional clean algorithm

Qualitative algorithm

Quantitative algorithm

Qualitative change

Graph 4 Types of energy­informational changes in IDS­M (Impedance Data System M) of Rm KM (Regime of Complex Meridians)

DiscussionWe have assumed that ancient points, so far, own only those elements’ characteristics which have been assigned to them in the past. E. g. LU 5 owns

elements’ characteristic of METAL and WATER. Already in Korean acupuncture according to Sa Am it’s mentioned that acupuncture points own more elements’ characteristics.[3]

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Clinic and ResearchThe Time Curvature in Acupuncture, Application in Clinic

Teodor Mochnáč, M. D., PhD.

Other authors prefer therapy, which uses the morphological characteristic of the acupuncture point to remove the pathological state.[6]

We assume that each acupuncture point owns all five element characteristics, which we can use in the treatment in order to influence the character of interactions and quantitative value of all five elements. This attribute results from relations of antique points with the elements’ characteristics, with the characteristics of the main and also complex meridians and with the characteristics of the meridians’ potency expressed by trigrams. The  findings reflected in the generalized and paired super­algorithms in RmKM are considered as a serious state and their improvement to the equilibrium state as a successful treatment. It correlates with the clinical improvement.

ConclusionThis study is a confirmation of the correctness of therapy using one acupuncture point, which according to Teo Mo Hexagram Sequence model. The  application of the model in the group of patients mentioned above represents the 89% success rate  i. e. correction of the tactile test, which correlates with the clinical improvement of the patients’ condition as well.

Teodor Mochnáč, M. D., PhD.AKUPUNKTUM, Center of Acupuncture and Diabetic footPod vinohradmi 14 A, 949 01 Nitra, Slovak RepublicE-mail: [email protected]

References1. Solárová, Z.: Selected Relations in the MKBD­S

Questionare. (Korngold­Beinfield Questionare Modified by the Author). In Acupuncture and Natural Mediucine, 2014, 2, p. 24–29, MSNM, Bratislava, ISSN 1339­4703.

2. Solárová, Z.: Theoretical Basic for the Diagnostic Process in the MKBD­S Questionare. (Korngold­Beinfield Questionare Modified by the Author of the Article). In Acupuncture and Natural Mediucine, 2014, 1, p. 32–38, MSNM, Bratislava, ISSN 1339­4703.

3. Yeong, Y.: Korean Medicine. Bratislava, MSNM, In Acupuncture and Natural Mediucine, 2013, 2, p. 14–26, ISSN 1339­4703.

4. Mochnáč, T.: The Time Curvature in the Acupuncture. Hexagram Sequences and their Interconnections, Part II. Bratislava, MSNM, In Acupuncture and Natural Mediucine, 2015, 4, p. 38–44, ISSN 1339­4703.

5. Mochnáč, T.: The Time Curvature in the Acupuncture. In Acupuncture and Natural Mediucine, 2015, 3, p. 15–18, MSNM, Bratislava, ISSN 1339­4703.

6. Maciocia, G.: The Channels of Acupuncture: Clinical Use ofthe Secondary Channels and Eight Extraordinary Vessels. Churchill Livingstone, 2006, p. 738, ISBN 10 0­443­07491­7, ISBN 13 978­0­443­07491­2.

7. Mochnáč, T.: Rizikové faktory a funkcie komplexných akupunktúrnych dráh vo vzťahu k ochoreniam prsnej žľazy. Bratislava, 2011, dizertačná práca, Vysoká škola zdravotníctva a sociálnej práce sv. Alžbety, Katedra verejného zdravotníctva, (s. 70 – 71).

8. Mochnáč, T.: TST DS­M v akupunktúre. Dizertačná práca, 2015, 1, LSNM, Bratislava, 2011, ISSN 1339­4703.

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Clinic and ResearchExperimental and Clinical Concept Essentials of Alloplant Regenerative Medicine

Prof. Ernst Rifgatovich Muldashev, M. D., DrSc., Radik Fayazovich Galiyakhmetov, M. D., CSc.

Experimental and Clinical Concept Essentials of Alloplant Regenerative MedicineProf. Ernst Rifgatovich Muldashev, M. D., DrSc., Radik Fayazovich Galiyakhmetov, M. D., CSc.

SummaryThe aim of the work is to compare morphogenetic effect of two methods of Alloplant biomaterial injectional insertion, that of local (perilimbal) and  acupunctural  one. Three series of the experiments have been carried out to achieve that goal. A standard chemical corneal burn without a subsequent treatment was inflicted in the control series. In  the first experimental series dispersed Alloplant biomaterial (DAB) was  perilimbally inserted against the background of the burn. In  the second experimental series DAB was inserted into paraorbital acupunctural points also following the burn. In this case it is shown, that the using of the local (perilimbal) and  the acupuncture application DBA activate reparative regeneration processes in the epithelium and the corneal stroma.

Key Wordsdispersed Alloplant biomaterial, corneal burn, regeneration

IntroductionWhen treating urgent corneal lesions various types of biological materials are being used. It  was academician  V. P. Filatov in Odessa eye research institute who had actively developed those technologies.

Aim of the WorkTo compare morphogenetic effect of two methods of Alloplant biomaterial injectional insertion, that of local (perilimbal) and acupunctural one.

Material and MethodsThe experimental part of the work was carried out in the Russian Eye and Plastic Surgery Centre of the Russian Health Ministry. Pathophysiological experiments were performed on 49 grey rabbits weighing 2  – 2.5  kg on the average. The  ex­periments were carried out in accordance with the acting international requirements. Since we planned to perform immunological investigations and study an immunological chain of the cor­neal chemical burn pathogenesis, we  selected exceptionally male­individuals who lacked cyclic harmonic changes typical to females. A chemical burn 6 mm in diameter was caused in the corneal centre by  2.5  % sodium hydroxide solution with 30  seconds exposition as per the standard procedure (Babushkin, A. E., Khafizov, G. G., 1996). The  animals were divided into 3  groups following the inflicted trauma. The control group (n = 7) was composed of the animals who were not then affected by any other interference. A  subconjunctival perilimbal injection of 1  ml dispersed allogeneic biomaterial (DAB) sus­pension, manufactured by the tissue bank of the Russian Eye and Plastic Surgery Centre, was  made to the animals of the experimental group  1 (n  =  10) following the inflicted burn. The graft is composed of such components of the connective tissue extracellular matrix as collagen (76.4  %), heparin sulphate (0.59  %), hyaluronic acid (2.42 %), chondroitin sulfate (0.44 %), other proteins (10.35 %), water (9.8 %). DAB in a vial made up 200 mg. That quantity was diluted in 5 ml physiological solution. Paraorbital projection

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Clinic and ResearchExperimental and Clinical Concept Essentials of Alloplant Regenerative Medicine

Prof. Ernst Rifgatovich Muldashev, M. D., DrSc., Radik Fayazovich Galiyakhmetov, M. D., CSc.

zones injected by  0.5  ml DAB solution were identified in the animals of experimental group 2 (n = 10). We have studied reactive changes of the local projection zones to investigate projection impact peculiarities of allogeneic dispersed biomaterial upon the corneal regeneration processes.

A  comparative analysis of cellular and vascular reactions in 22  animals was carried out when inserting DAB into projection zones (n  =  11) and  in case of ordinary acupunctural effect (n = 11). All the animals were sacrificed on the 3rd, 7th, 14th, 30th  day. The  cornea with the scleral adjacent part as well as the skin and subdermal tissues of DAB insertion zone were taken for the histological investigation. The  histological investigations were performed with the use of hematoxylin and eosin staining as per Mallory and van Gieson. The biomaterial resorption was studied by the quantitative estimate method of collagen structure anisotropic coefficient (AC) using polarizing microscopy of unstained histological sections, using the microscope Min­8  with digital photoadapter Nikon Coolpix 4500 with cross­filters. The information about optical active objects were analyzed with specialized program Biovision 3.0. The obtained data were converted into the Microsoft Excel 2003. Consequently there was performed the calculation of the ratio of overall area of optically active entities to the total area of the selected section and at the same time the deviations was reduced. The data were processed and plotted in the program Statistica 5.5.

Immunohistochemical investigations revealed two factor expressions: transforming growth factor (TGF  β­1) which was fibrosis inducer (Sporn  M.B., Roberts  A.B., 1990) and  nuclear antigen of proliferating cells (PCNA) which

allowed to estimate proliferative activity of cells (Yew,  D. T. et  al., 2000). TGF  β­1  and PCNA expression was determined by an indirect immunoperoxidase method on serial 5  mcm thick paraffin sections with the use of corresponding monoclonal antibodies (Santa Cruz Biotechnology  Inc.). A  mitotic index showing the correlation between the quantity of PCNA positive cells and 100  epitheliocytes was determined in the corneal epithelium cells. The  results estimate was carried out with “part calculation” function of Biovision  3.0  program. To  study reactive process dynamics in control and following DAB insertion, a  calculation of total capillary lumen area (TCLA) of  the limbal region was made on the histological preparations by the morphometrical method, using the program Biovision 3.0 and Professional and microscope MC­50, digital camera Nikon CP 4500 and PC. The skin impedance parameters in the local projection zones were determined in all investigated animals (n = 49) with the aid of a high­voltage impulse generator furnished with microprocessor control “AcuVision  – 04” (Russia). The device is manufactured by company Graviton (Moscow) and registered in the register of medical products (registration certificate No. 29/23010203/5270­03 dated May 20th 2003).

ResultsThe  experimental chemical burn in the zone of the direct contact with the detergent caused rough pathological changes in the animals, namely destruction of epithelium, basal membrane and superficial layers of the corneal proper substance. The  affection depth achieved 50  mcm. The  destructive processes were also revealed along the periphery from that region at the distance equal to 200  mcm on the average. It allowed us to specify that region as a marginal zone of the damage.

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One  of the main manifestations of the corneal damage was the reduction of AC which dropped on the 3rd  day (p  <  0.01) of  our experiment by merely 20  % compared with the standard parameter.

Changes in the form of the proliferation of keratoblasts, macrophages and leukocytes were also revealed on the border with the damage marginal zone at the distance up to 500  mcm. We designated that region as the zone of reactive changes.

Finally we didn’t reveal any changes in the corneal peripheral areas at the light optical level. It  is necessary to note that in case of the corneal trauma the revealed histotopographic zones corresponded on the whole to the data of other authors who had studied corneal experimental damages (Maksimov, I. B., 1990). According to the obtained results, manifestations and dynamics of reparative processes in the specified zones substantially depended upon the experimental series. Table  1 below provides summary quantitative data.

Tab. 1 Parameters of corneal reparative regeneration in case of local and acupunctural DAB insertion

Series Control Local insertion Acupunctural impactPeriod, days 3 7 3 7 3 7

Compared with Initial state Control Control TGFβ-1 expression parameters, % (M ± m) n = 27

Initial state2.57 ± 1.86

31.15 ± 1.86 40.3 ± 2.06 16.1 ± 2.13 2.27 ± 1.32 26.5 ± 1.45 4.74 ± 1.64

Level of reliance p < 0.01 p < 0.01 p < 0.05 p < 0.001 p > 0.05 p < 0.01Parameters of corneal epithelium mitotic index, % (M ± m) n = 27

Initial state30.6 ± 3.09

8.8 ± 3.50 25.4 ± 5.30 16.8 ± 4.42 36.4 ± 4.27 12.4 ± 2.95 29.3 ± 5.25

Level of reliance p < 0.05 p < 0.05 p < 0.05 p < 0.05 p < 0.05 p < 0.05Parameters of anisotropic coefficient, % (M ± m) n = 27

Initial state86.5 ± 5.34

16.7 ± 1.99 38.3 ± 4.08 46.5 ± 3.78 81.4 ± 2.85 38 ± 3.5 54.9 ± 3.41

Level of reliance p < 0.01 p < 0.05 p < 0.05 p > 0.05 p < 0.05 p < 0.05Parameters of total limbal capillary lumen area. mcm² (M ± m) n = 27

Initial state846 ± 161.1

1697 ± 130 2115 ± 102.9 2119 ± 161.1 2720 ± 110.2 1764 ± 113.6 2360 ± 104.5

Level of reliance p < 0.05 p < 0.05 p < 0.05 p < 0.05 p < 0.05 p < 0.05

DiscussionThe revealed similarity of the corneal reparative regeneration dynamics in our experiments, in case of perilimbal insertion of DAB and DAB effect by way of the acupuncture method was of the fundamental importance since the achievement of positive results on corneal regeneration by the effect upon the projection zones was more preferable compared with the surgical intervention, which in fact, was a DAB perilimbal

insertion. Experimental modelling of the corneal chemical burn resulted in tissue necrosis in the contact zone, succeeded by the development of inflammatory processes. The  disorder of the corneal proper substance structure was manifested by a reliable reduction of anisotropic coefficient on the 7th  day up to 44  % from the initial level. Normalizing anterior epithelium took place on the 14th  day of the experiment and proliferative activity of epitheliocytes was

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restored on the 7th day. DAB acupunctural effect rationalized the mechanisms of the corneal reparative regeneration which was testified by normalizing mitotic index on the 7th  day of the experiment.

The transforming growth factor expression in the corneal proper substance achieved its standard parameter within the same period reducing the probability of fibrosis development in the zone of the chemical burn. Anisotropy coefficient of collagen fibers achieved 63  % of the standard parameter on the 7th day reflecting the restoration dynamics of the functionally adequate structures. The  carried out experimental investigations showed that both local and acupunctural DAB insertion had been an effective stimulation method of reparative processes both in the stroma and corneal epithelium (tab.  1). A  comparative analysis of two experimental series showed, in  particular, that both DAB perilimbal insertion and its effect mobilized a complex of vascular and cellular reactions which were evident as a polymorphous infiltration involving macrophages and angiogenesis processes with subsequent resorption of the fibrous biomaterial matrix. The  results of our investigations on activation of acupunctural points when DAB inserting were published in the journal “Acupuncture and Natural Medicine (No. 1, 3, 2015)”. Clinical tests using Alloplant technologies fully supported to the efficiency of DAB local insertion and pharmacopuncture in the treatment of different lesions of the organ of vision, degenerative processes of the peripheral nervous system (polyneuropathy of different etiology), ENT  (ear, nose, throat) diseases, sexopathology. The  pharmacopuncture method with Alloplant biomaterial have also showed positive results in the treatment of a number of demyelinating diseases of the central nervous system, consequences of traumas, systemic lesions of connective tissue and other pathological states.

ConclusionUnfortunately, we  had no way to carry out a comparative analysis since the experiments on Alloplant biomaterial acupunctural insertion had been performed only in our Centre. We also didn’t have the data of other authors relating to the results of clinical pharmacopuncture in ophthalmology by Alloplant biomaterial.

Experience gained in our Centre’s reflexotherapy laboratory made it possible to suggest the following practical recommendations of using Alloplant biomaterial injectional forms:

– acupunctural and local Alloplant biomaterial insertion may be used to stimulate reparative regeneration of different anatomical structures. The  lesions of the peripheral nervous system as well as disorders of the neuromuscular condition, various pathologies of sense organs could be indications for Alloplant biomaterial pharmacopunture;

– injectional form of Alloplant biomaterials maintaining local vascular and cellular reactions for a long period of time may be used in pharmacopunctural therapy taking into account their prolonged effect and subsequent full resorption with no fibrosis phenomena.

The obtained results of the experiment have been realized in clinical practice of the Russian Eye and Plastic Surgery Centre and in a number of clinics of traditional medicine and rehabilitation. Thus, this investigation can be regarded as an aggregated experimental and clinical work revealing the regularities of morphofunctional changes in transplanting dispersed forms of biomaterials.

It  is also necessary to note that the given phar­macopuncture procedure is used as an inde­pendent method of treatment regardless of the

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surgical intervention. Investigations in this field of medicine are being continued by the scientists of the Russian Eye and Plastic Surgery Centre.

Professor Ernst Rifgatovich Muldashev, M. D., PhD.Director General of the Russian Eye and Plastic Surgery Centre, 67/1 Zorge str., Ufa, 450075, Russian FederationE-mail: [email protected]

Radik Fayazovich Galiyakhmetov, M. D., PhD.Head of the Department of Restorative Medicine, the Russian Eye and Plastic Surgery Centre, 67/1 Zorge str., Ufa, 450075, Russian FederationE-mail: [email protected]

References1. Nigmatullin, R. T., Gafarov, B. G.,

Galiyakhmetov, R. F., Mukhametov, A. R., Aprelev, A. E., Sherbakov, D. A.: Certain Regularities of the Graft Biodegradation. Morfologicheskie Vedomosti, 2008, 3, 4, p. 130–132, ISSN­1812­3171.

2. Muldashev, E. R., Galimova, V. U., Nigmatullin, R. T., Kirillichev, A. I., Aprelev, A. E., Galiyakhmetov, R. F.: Reactive Changes in Hypoderma when Inserting Alloplant biomaterial. Morfologicheskie Vedomosti, 2008, 3, p. 77–78, ISSN­1812­3171.

3. Muldashev, E. R., Galiyakhmetov, R. F.: Experimental Justification for Using Dispersed Forms of Allografts in Acupuncture. In Acupuncture and Natural Medicine, MSNM, Bratislava, 2015, 1, p. 22–26, ISSN­1339­4703.

4. Muldashev, E. R., Nigmatullin, R. T., Galiyakhmetov, R. F., Aprelev, A. E.: Morphological Aspects of Pharmacopuncture by Alloplant Biomaterials. In Acupuncture and Natural Medicine, MSNM, Bratislava, 2015, 3, p. 19–24, ISSN­1339­4703.

Original article in Russian language:

Экспериментальные и клинические основы концепции регенеративной медицины АллоплантПроф. Ернст Рифгатович Мулдашев, доктор медицинских наук, Радик Фаязович Галиачметов, доктор медицинских наук

АннотацияЦелью работы является сравнительный ана­лиз результатов лечения химического ожога роговицы в эксперименте. Для этого выполне­ны три серии экспериментов. В контрольной серии наносился стандартный химический ожог роговицы без последующего лечения. В первой опытной серии на фоне ожога пери­лимбально вводился диспергированный био­материал Аллоплант (ДБА). Во второй опыт­

ной серии также после ожога ДБА вводился в  параорбитальные акупунктурные точки. При этом показано, что как локальное (пери­лимбальное), так  и акупунктурное введение ДБА активирует процессы репаративной ре­генерации в эпителии и строме роговицы.

Ключевые словаДиспергированный биоматериал Аллоплант, ожог роговицы, регенерация.

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Prof. Ernst Rifgatovich Muldashev, M. D., DrSc., Radik Fayazovich Galiyakhmetov, M. D., CSc.

ВведениеЭкспериментальная часть работы прово­дилась во «Всероссийском центре глазной и пластической хирургии» Министерства здравоохранения Российской Федерации. При  лечении ургентных поражений рогови­цы используются самые различные виды био­логических материалов. Данные технологии активно разрабатывались начиная с  трудов академика  В. П. Филатова в  Одесском науч­но­исследовательском институте глазных бо­лезней.

Цель настоящей работы: сравнить мор­фо­генетическое действие двух методов инъекционного введения биоматериала ал­лоплант  — локального (перилимбального) и акупунктурного.

Материалы и методы исследованияПатофизиологические опыты проведены на 49  серых кроликах средним весом  2–2,5  кг. Эксперименты были проведены в  соответ­ствии с  действующими международными требованиями.Поскольку планировалось проведение имму­нологических исследований и изучение имму­нологического звена патогенеза химического ожога роговицы, выбирались исключительно особи мужского пола, у которых отсутствуют циклические гормональные изменения, ха­рактерные для самок.

Химический ожог диаметром 6  мм в  центре роговицы вызывали  2,5% раствором едкого натра 30­секундной экспозицией по стандарт­ной методике (Бабушкин  А. Э., Хафизов  Г. Г., 1996). После нанесения травмы животные были разделены на 3  группы. Контрольную группу (n = 7) составили животные, которым после нанесения ожога каких­либо других воздействий больше не проводили. В  экспе­

риментальной группе 1 (n = 10), после нанесе­ния ожога животным производили субконъ­юнктивальную перилимбальную инъекцию суспензии 1  мл диспергированного аллоген­ного биоматериала (ДБА), произведенного тканевым банком Всероссийского центра глазной и пластической хирургии. В составе трансплантата присутствуют такие компо­ненты внеклеточного матрикса соединитель­ной ткани как коллаген (76,4 %), гепарансуль­фат (0,59  %), гиауроновая кислота (2,42  %), хондроитинсульфат (0,44  %), прочие белки (10,35 %), вода (9,8 %).

Навеска в одном флаконе составляла 200 мг. Указанное количество разбавляли 5 мл физи­ологического раствора. В экспериментальной группе 2 (n = 10) у животных идентифициро­вали параорбитальные зоны проекции, в ко­торые инъецировали по 0,5 мл раствора ДБА.Для  исследования особенностей акупун­ктурного воздействия аллогенного диспер­гированного биоматериала на процессы ре­генерации роговицы нами были изучены реактивные изменения в локальных рефлек­согенных зонах. У 22 животных был проведен сравнительный анализ клеточных и сосуди­стых реакций при введении ДБА в зоны про­екции (n = 11) и при акупунктурном воздей­ствии (n = 11).

Всех животных выводили из опыта на 3, 7, 14, 30­е сутки. Для гистологического исследова­ния забирали роговицу с прилежащей частью склеры, а также кожу и субдермальные ткани в области введения ДБА.

Гистологические исследования проводили с  использованием окраски гематоксилином и эозином, а  также по методам Маллори и Ван­Гизона.

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Резорбцию биоматериала изучали мето­дом количественной оценки коэффициента анизотропии (КА) коллагеновых структур с  помощью поляризационной микроскопии неокрашенных гистологических срезов ми­кроскопом Мин. 8 с цифровой фотонасадкой Nicon Coolpix  4500 при скрещенных филь­трах. Полученная информация об оптиче­ски активных объектах анализировалась с помощью специализированной программы Biovision  3.0. Полученные данные переводи­лись в программу Microsoft Office Excel 2003, где производился расчёт отношения суммар­ной площади оптически активных объектов к общей площади выбранного участка, а так­же устранялись погрешности. Затем обрабо­танные данные переводились в  программу Statistica  5.5. для  построения соответствую­щих графиков.

С  помощью иммуногистохимических ис­следований выявляли экспрессию двух фак­торов: трансформирующего фактора роста (TGF  β­1), который является индуктором фиброза (Sporn, M. B., Roberts, A. B., 1990) и ядерного антигена пролиферирующих кле­ток (PCNA), позволяющего судить о  проли­феративной активности клеток (Yew, D. T. et  al., 2000). Экспрессию TGF β­1  и PCNA определяли непрямым иммунопероксидаз­ным методом на серийных парафиновых сре­зах толщиной 5 мкм с использованием соот­ветствующих моноклональных антител (Santa Cruz Biotechnology Inc.).

Митотический индекс, как  количество PCNA­положительных клеток на 100  эпите­лиоцитов, определяли в клетках эпителия ро­говицы. Оценка результатов производилась с  помощью функции «подсчет части» про­граммы Biovision 3.0.

Для изучения динамики реактивных процес­сов в контроле и после введения ДБА на гисто­логических препаратах производили подсчет суммарной площади просвета капилляров (СППК) лимбальной области морфометри­ческим методом с  использованием програм­мы Biovision  3.0 Professional и аппаратного комплекса, включающего исследовательский микроскоп МС­50, цифровой фотоаппарат Nikon CP 4500 и персональный компьютер.

Показатели кожного импеданса в локальных рефлексогенных зонах определяли у  всех исследованных животных (n  =  49) с  помо­щью высоковольтного импульсного гене­ратора с  микропроцессорным управлением «AcuVision­04» (Россия). Прибор произво­дится научно­производственным предпри­ятием «Гравитон» (Москва) по  требованиям утвержденных технических условий, зареги­стрирован в Российской Федерации и внесен в  реестр медицинских изделий (Регистраци­онное удостоверение № 29/23010203/5270­03 от 20 мая 2003 года.

РезультатыЭкспериментальный химический ожог в зоне непосредственного контакта с  детергентом у  всех животных вызывал грубые патологи­ческие изменения  — деструкцию эпителия, базальной мембраны и поверхностных слоев собственного вещества роговицы. Глубина поражения достигала 50 мкм. По периферии от этой области на расстоянии, составляю­щем в  среднем 200  мкм, также обнаружива­лись деструктивные процессы, что позволило нам определить эту область как краевую зону повреждения.

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Одним из основных проявлений поврежде­ния роговицы является снижение коэффи­циента анизотропии (КА), падение которого в  наших экспериментах достигало 20  % от нормы на 3­и сутки (p < 0,01).

На  границе с  краевой зоной повреждения, на расстоянии до 500 мкм также были выяв­лены изменения в виде пролиферации кера­тобластов, макрофагов, лейкоцитов. Эту  об­ласть мы обозначили как зону реактивных изменений.

И, наконец, в периферических участках рого­вицы на светооптическом уровне изменений не обнаружено. Следует отметить, что  об­наруженные нами гистотопографические зоны при ожоговой травме роговицы в  це­лом соответствуют данным других авторов, изучавших экспериментальное повреждение роговицы (Максимов, И. Б., 1990). Соглас­но полученным результатам выраженность и динамика репаративных процессов в  ука­занных зонах существенно зависела от серии экспериментов, обобщенные количественные данные представлены в табл. 1.

Табл. 1 Показатели репаративной регенерации роговицы при локальном и рефлексогенном введении дба

Серия Контроль Локальное введение Рефлексогенное воздействие

Сроки, сутки 3 7 3 7 3 7В сравнении с исходным состоянием с контролем с контролем

Показатели экспрессии TGFβ-1, % (M ± m) n = 27Исходное состояние

2,57 ± 1,8631,15 ± 1,86 40,3 ± 2,06 16,1 ± 2,13 2,27 ± 1,32 26,5 ± 1,45 4,74 ± 1,64

Уровень доверия p < 0,01 p < 0,01 p < 0,05 p < 0,001 p > 0,05 p < 0,01Показатели митотического индекса эпителия роговицы, % (M ± m) n = 27

Исходное состояние30,6 ± 3,09

8,8 ± 3,50 25,4 ± 5,30 16,8 ± 4,42 36,4 ± 4,27 12,4 ± 2,95 29,3 ± 5,25

Уровень доверия p < 0,05 p < 0,05 p < 0,05 p < 0,05 p < 0,05 p < 0,05Показатели коэффициента анизотропии, % (M ± m) n = 27

Исходное состояние86,5 ± 5,34

16,7 ± 1,99 38,3 ± 4,08 46,5 ± 3,78 81,4 ± 2,85 38 ± 3,5 54,9 ± 3,41

Уровень доверия p < 0,01 p < 0,05 p < 0,05 p > 0,05 p < 0,05 p < 0,05Показатели суммарной площади просвета капилляров лимба, мкм² (M ± m) n = 27

Исходное состояние846 ± 161,1

1697 ± 130 2115 ± 102,9 2119 ± 161,1 2720 ± 110,2 1764 ± 113,6 2360 ± 104,5

Уровень доверия p < 0,05 p < 0,05 p < 0,05 p < 0,05 p < 0,05 p < 0,05

ДискуссияОбнаруженное в наших экспериментах сход­ство динамики репаративной регенерации роговицы при перилимбальном введении диспергированного аллогенного биомате­риала и воздействие ДБА акупунктурнным путем имеет принципиальное значение, по­

скольку достижение позитивных результатов по регенерации роговицы путем воздействия на зоны проекции является более предпочти­тельным по сравнению с  оперативным вме­шательством, каковым, по сути, является пе­рилимбальное введение ДБА.

Acupuncture and Natural Medicine 5–6/2015 30

Clinic and ResearchExperimental and Clinical Concept Essentials of Alloplant Regenerative Medicine

Prof. Ernst Rifgatovich Muldashev, M. D., DrSc., Radik Fayazovich Galiyakhmetov, M. D., CSc.

Экспериментальное моделирование хими­ческого ожога роговицы приводит к  некро­зу тканей в контактной зоне с последующим развитием воспалительных процессов. На­рушение структуры собственного вещества роговицы проявляется достоверным сниже­нием коэффициента анизотропии до 44 % от исходного уровня на 7­е сутки. Нормализа­ция переднего эпителия происходит на 14­е сутки эксперимента, а  пролиферативная ак­тивность эпителиоцитов восстанавливается на 7­е сутки.

Акупунктурное воздействие диспергирован­ным аллогенным биоматериалом оптимизи­рует механизмы репаративной регенерации роговицы, о чем свидетельствует нормализа­ция митотического индекса на 7­е сутки экс­перимента. В эти же сроки достигает нормы экспрессия трансформирующего фактора ро­ста в  собственном веществе роговицы, сни­жая вероятность развития фиброза в зоне хи­мического ожога. Коэффициент анизотропии коллагеновых волокон на 7­е сутки достигает 63% от нормы, отражая динамику восстанов­ления функционально адекватных структур.Таким образом, проведенные эксперимен­тальные исследования показали, что  и ло­кальное, и  акупунктурное введение алло­генного диспергированного биоматериала является эффективным методом стимуляции репаративных процессов как в строме, так и в эпителии роговицы (табл. 1).

В  частности, сравнительный анализ резуль­татов двух серий экспериментов показал, что как перилимбальное введение ДБА, так и его воздействие на зоны проекции мобилизу­ют комплекс сосудистых и клеточных реак­ций, которые проявляются в  полиморфной инфильтрации с участием макрофагов и про­цессами ангиогенеза с последующей резорб­цией волокнистого матрикса биоматериала.

Результаты наших исследований по актива­ции акупунктурных точек при введении ДБА опубликованы в  отдельных статьях журна­ла «Акупунктура и натуральная медицина» (№ 1, № 3, 2015).

Клинические испытания представляемой технологии Аллоплант полностью подтвер­дили высокую эффективность локального введения ДБА и фармакопунктуры при ле­чении различных поражений органа зрения, дегенеративных процессах периферической нервной системы (полинейропатии различ­ной этиологии), заболеваниях ЛОР­органов, сексопатологии.Метод фармакопунктуры с  использованием биоматериалов Аллоплант также продемон­стрировал положительные результаты при лечении целого ряда демиелинизирующих заболеваний центральной нервной системы, последствий травм, системных поражениях соединительной ткани и других патологиче­ских состояниях.

ЗаключениеК сожалению, мы не имеем возможности про­вести сравнительный анализ, так  как экспе­рименты по акупунктурному введению био­материала Аллоплант проводились только в нашем Центре. В нашем распоряжении так­же нет данных других авторов по результатам клинической фармакупунктуры биоматериа­лом Аллоплант в офтальмологии.

Накопленный клинический опыт в  лабора­тории рефлексотерапии нашего Центра по­зволил сформулировать следующие прак­тические рекомендации по использованию инъекционных форм биоматериалов Алло­плант:

– акупунктурное и локальное введение био­материалов Аллоплант может быть ис­пользовано для стимуляции репаративной

Acupuncture and Natural Medicine 5–6/2015 31

Clinic and ResearchExperimental and Clinical Concept Essentials of Alloplant Regenerative Medicine

Prof. Ernst Rifgatovich Muldashev, M. D., DrSc., Radik Fayazovich Galiyakhmetov, M. D., CSc.

регенерации различных анатомических структур. Показаниями для фармакопун­ктуры биоматериалом Аллоплант могут являться поражения периферической нервной системы, в  том числе с  наруше­ниями нервно­мышечной проводимости, разнообразная патология органов чувств.

– инъекционная форма биоматериалов Ал­лоплант, длительно поддерживая локаль­ные сосудистые и клеточные реакции, может использоваться в  схеме фармако­пунктурной терапии с учетом их пролон­гированного эффекта и последующей пол­ной резорбции без явлений фиброза.

Полученные в  эксперименте результаты ре­ализованы в  клинической практике Всерос­сийского центра глазной и пластической хи­рургии и целого ряда клиник традиционной медицины и реабилитации. Таким образом, настоящее исследование можно рассматри­вать как комплексную экспериментальную и клиническую работу, раскрывающую зако­номерности морфофункциональных измене­ний при трансплантации диспергированных форм биоматериалов. Следует также отме­тить, что данная методика фармакопунктуры применяется и как самостоятельный метод лечения независимо от хирургического вме­шательства. В настоящее время продолжают­ся многоцентровые исследования в  рамках представляемой комплексной темы с участи­ем специалистов Всероссийского центра глаз­ной и пластической хирургии.

Результаты наших исследований по актива­ции акупунктурных точек при введении ДБА опубликованы в отдельных статьях журнала «Акупунктура и натуральная медицина».

Проф. Ернст Рифгатович Мулдашев, доктор медицинских наук, Радик Фаязович Галиачметов, доктор медицинских наукФедеральное государственное бюджетное учреждение «Всероссийский центр глазной и пластической хирургии» Министерства здравоохранения Российской Федерации, ул. Зорге 67/1, 450075 УфаE-mail: [email protected]: [email protected]

Литература1. Нигматуллин, Р. Т., Гафаров, В. Г.,

Галиахметов, Р. Ф., Мухаметов, А. Р., Апрелев, А. Е., Щербаков, Д. А.: Некоторые закономерности биодеградации трансплантатов. Морфологические ведомости, 2008, № 3–4, с. 130–132, ISSN­1812­3171.

2. Мулдашев, Э. Р., Галимова, В. У., Нигматуллин, Р. Т., Кирилличев, А. И., Апрелев, А. Е., Галиахметов, Р. Ф.: Реактивные изменения в гиподерме при введении биоматериала Аллоплант. Морфологические ведомости, 2008, № 3, с. 77–78, ISSN­1812­3171.

3. Muldashev, E. R., Galiyakhmetov, R. F.: Experimental Justification for Using Dispersed Forms of Allografts in Acupuncture. Acupuncture and Natural Medicine, Bratislava, 2015, № 1., с. 22–26, ISSN­1339­4703.

4. 4. Muldashev, E. R., Nigmatullin, R. T., Galiyakhmetov, R. F., Aprelev, A. E.: Morphological Aspects of Pharmacopuncture by Alloplant Biomaterials. Acupuncture and Natural Medicine, Bratislava, 2015, № 3, с. 19–24, ISSN­1339­4703.

Acupuncture and Natural Medicine 5–6/2015 32

Clinic and ResearchValues of Intraocular Pressure and Acupuncture – Pilot Study

Magdaléna Miklósová, M. D.

Values of Intraocular Pressure and Acupuncture – Pilot Study

Magdaléna Miklósová, M. D.

SummaryThe  author evaluates the effect of acupuncture treatment on intraocular pressure (IOP) of 80 eyes of 40  patients without an acute eye disease and glaucoma. Patients visited First clinic of acupuncture and natural medicine of G. Solar s.r.o. from October 2014 until September 2015 (1 day in every month). Average value of IOP of right eye bulb  – RE  (left eye bulb  – LE) before the acupuncture treatment was  16.5  mmHg; standard deviation­SD 2.6 mmHg (16.3 mmHg; SD  3.3  mmHg) with a maximal value of IOP  22.0  mmHg (22.0  mmHg) and  minimal value of IOP 9  mmHg (6  mmHg). Average value of IOP RE (LE) after the acupuncture treatment was  15.1  mmHg; SD  1.9  mmHg (14.0 mmHg; SD 2.7 mmHg) with maximal value of IOP  19.0  mmHg (21.0  mmHg) and  minimal value of IOP 14.0 mmHg (8 mmHg). In the group, IOP of RE decreased by 8.5 %, IOP of LE by 14 % and together by  11.4  %. Decrease of IOP was seen in 52 cases (65 %), increase of IOP in 9 cases (11 %) and in 19 cases (24 %) no difference was detected between the values measured before and after the treatment. The  pilot study proved that acupuncture has statistically significant effect of IOP (P < 0.01) and its effect on IOP is of regulative nature.

Key Wordsintraocular pressure, glaucoma, acupuncture, natural medicine, energy­informative processes

IntroductionPhysiological intraocular pressure (IOP) enables the right function of the eye. Normal value of IOP is the statistic range of population’s IOP (does not need to apply for the individual). Average IOP of Central Europe’s population is 16  mmHg (11–21  mmHg).[3] Boundary value of IOP of adults (21  mmHg) is  specific with children. In  case of small children, extensive differences exist measured while alert and in narcosis.[6] Average value of IOP of children is  8–13  mmHg and it increases by 1 mmHg every 2 years from the birth until 12 years of age.[3] The following factors have influence on the measured IOP:

– diurnal variation of IOP  – physiological: 3–5 mmHg, IOP has circadian cycle

– thickness and curve of cornea, position of the patient

– consensual fluctuation: the  maximum difference between the IOP of right and left eye bulb is 5 mmHg

– this fluctuation is related to the heartbeat, respiration movements.

The value of IOP is determined by the production of aqueous humor, trabecular and uveoscleral outflow and episcleral venous pressure. The increased or unstable IOP is the major risk factor of glaucoma. It is a multifactorial chronic progressive neuropathy of the optic nerve related to the damage of retinal ganglion cells, which

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Magdaléna Miklósová, M. D.

leads to changes of the optic nerve head (ONH) and  in visual field.[3] Consequences of high IOP are:

– mechanical oppression of axons in the area of lamina cribrosa of ONH and consequently damage of nerve fibre with disruption of axoplasmic transport (mechanical theory)

– higher IOP can decrease circulation or it damages veins by oppression, microinfarcts (joining of vascular and mechanical theory).[8]

At present time, treatment of glaucoma consists of decreasing of  IOP, but  it is important to increase the perfusion pressure (secondary, indirect neuroprotection), as  well as direct neuroprotection.

Therapy of glaucoma can be: – conservative

pharmacological: antiglaucomaticsnon­pharmacological: natural antioxidants, acupuncture[4]

– laser  – iridotomy, iridoplastics, trabeculo­plastics, cyklophotocoagulation

– surgical  – filtrating (perforation and nonperforation), draining and cyklodestruc­tive operations.

Acupuncture is a branch of medicine. It  studies and uses energy­informative processes which are universal in the whole cosmos. Acupuncture as a key branch of natural medicine is preventive, diagnostic, therapeutic and researching application of traditional and modern knowledge of active points, meridians and microsystems in prevention and therapy of malfunctions and diseases.[15]

From the traditional acupuncture point of view, the  eye (sense organ of vision) as  well as gall­ bladder, tendons, connective tissue and nails belong to the organ system of liver, which is assigned to the WOOD element.[1] In  modern

acupuncture, the  element is characterized as definite, multi­grade, universal energy­informative level.

The  WOOD element is an energy­informative aspect, it is characterized by embranchment and growth. WOOD has a yin meridian HE (hepar) – directing organ: liver and a yang meridian VF (vesica fellea) – directing: gall­bladder. According to theory of WU­XIN, every element gives birth to another  one. WOOD element is determined in the birth cycle of triplet WATER­WOOD­FIRE (beginning­peak­transition). Meridians of WOOD element – HE and VF are organized in different triplets, e.g. according to circadian rhythm.[17]

The  eye has direct and indirect (through meridians HE, VF) functional connections to all main meridians, as well as to the whole subsystem of 8  complex meridians. Every meridian affects primarily its organ circle, but  its action in organism is complex.[17] Every single meridian has several direct connections to other meridians, but these connections have fixed order thus they form an energy­informative network[18] which is part of the whole energy­informative system of microcosm (and macrocosm). This rich energy­informative network can explain fluctuation of IOP between right eye and left  eye, as  well as the existence of the huge number of diagnoses involving the eye directly.

The  organ system of liver (+  gall­bladder, eye, tendons, connective tissue and nails) composes biochemical­morphologic level of WOOD element. Psycho­regulative level interprets what the eye sees on the level of consensual reality. Meridian HE is characterized by emotivity, motivation (high, fluctuating  IOP), meridian VF directs resolvedness, defines the extent of anger and fear­resistance (increased or decreased sensitivity for negative effects  – fluctuation

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Magdaléna Miklósová, M. D.

of IOP). Energy­informative level is characterized by INTERSECTION (wind)  – to  see things right, to gain overview and wisdom in decision­making, DYNAMICS (storm) and  transitority (fluctuation of IOP). The highest level is spiritual, in  which  – while examining etiopathogenic diseases – answers to the question WHY? can be found.

The  eye  – eyeball +  accessory organs (pic.1): extraocular muscles, blood vessels, lacrimal mechanism, eyelids, optic nerve belong to the 5  elements and together they form a full pentagram (energy­ interactive system).

Fig. 1 The eyeball and its accessory organs with the five elements marked (the eyeball represents the element WOOD – D, blood vessels: FIRE – O, extraocular muscles: EARTH – Z, eyelids: METAL – K, optic nerve: WATER – V).

Pic.  2 also schematically shows the transversal section through the orbit and distribution of ocular structures, which correlate with meridians

of vertical plane of pyramid model in acupuncture. Meridian HE (hepar) with meridians IC (intestinum crassum), VE  (ventriculus), VU  (vesica urinaria) and  CO (cor) forms the vertical structure of the pyramid.[19]

Fig. 2 “Ophtalmological” application of the pyramid model in acupuncture (structures of the orbit – blood vessels, optic nerve, eyeball, extraocular muscles and skin of the eyelid represent meridians CO – cor, VU – vesica urinaria, HE – hepar, VE – ventriculus a IC – intestinum crassum, which are shown on the central vertical axis of the pyramid).

After projecting the Universal Octagram onto the periocular area (entrance into the orbit) we obtain the horizontal plane of the pyramid model and, as a periocular rotational microsystem it can be used in acupuncture treatment and diagnosis (one of Tactile Solar Test – TST) – Pic. 3.

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Clinic and ResearchValues of Intraocular Pressure and Acupuncture – Pilot Study

Magdaléna Miklósová, M. D.

Fig. 3 eriocular rotational microsystem (projection of the universal octagram onto the periocular region: YIN concatenations are created by meridians LP – lien pancreas, PU – pulmo, TC – tricalorium, PE –pericard – 1, 4, 6, 7 and YANG concatenations are created by meridians IT – intestinum tenue, VF – vesica fellea, RE – ren, VU – vesica urinaria – 2, 3, 5, 8).

Pic.  1, 2, and  3  show the application of the pentagram and pyramid model of acupuncture to the  eye: The  entire eye appears in both planes of the pyramid model, and  the entire energy­informative system appears in the eye (in  projection of the elements)  – system in a system.

Model of circadian rhytm

18–21 Min. IOP Min. IOPMax. IOP 06–09

Circadian cycle of IOP

Fig. 4 Model of circadian rhythm in acupuncture (upper half of the picture) and circadian cycle of IOP – intraocular pressure (lower half of the picture). (01 to 23 hours of the day, meridians: CO – cor, IT – intestinum tenue, VU – vesica urinaria, RE – ren, PE – pericard, TC – tricalorium, VF – vesica fellea, HE – hepar, PU – pulmo, IC – intestinum crassum, VE – ventriculus, LP – lien pancreas, Min.IOP – minimal values of IOP, Max.IOP – maximal values of IOP).

Model of circadian rhythm in acupuncture and circadian cycle of IOP are shown together in Pic. 4, as there are some correlations between them: Meridian VF dynamically “starts” the production of aqueous humor , yin  component is managed by meridian HE in harmonious (meridian IT  – intestinum tenue) cooperation with cardiovascular system (meridian  CO). Value of IOP physiologically increases, and after reaching maximal values during morning hours IOP gradually decreases. Aqueous humor (AH) is produced in the posterior chamber and ensures nutrition of avascular structures of anterior eye segment (meridians VE­ventriculus, LP  – lien pancreas, dynamics are managed by meridian TC – tricalorium). AH circulates from posterior chamber across the pupil to anterior chamber and flows to a greater extent via trabeculum

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Clinic and ResearchValues of Intraocular Pressure and Acupuncture – Pilot Study

Magdaléna Miklósová, M. D.

and Schlemm’s canal to episcleral veins  – (the movement of water is managed by meridians PU – pulmo, RE – ren, VU – vesica urinaria).

Aim  of Study: To  detect the influence of acupuncture and character of its effect on the values of intraocular pressure (IOP).

MethodologyIn  the study, data of 40  patients (80  eyes)  – 29  women, 11  men (graph  1) were evaluated. They were patients of First clinic of acupuncture and natural medicine of G. Solar  s. r. o. (further clinic), who visited the clinic from October 2014 until September 2015 (third or fourth Thursday in month between 4  pm and 9  pm). They had different health problems (vertebrogenic, artralgies, migraines and other headaches), but did not have an acute eye disease or glaucoma. Average age of patients was 40.5 years (standard deviation  – SD  9.6  y.), while the oldest patient was 59, the youngest 14 years old.

72.5 %

27.5 %

men

women

Graph 1 Percentage distribution of studied group by gender

In  every patient IOP was measured before and after the acupuncture treatment. Acupuncture examination and treatment was made by the team of the clinic. The  complete acupuncture examination (tongue diagnostics, pulse auriculodiagnostics, Su­Jok, electroacugraphy  – EAG, complex whole­body TST diagnostics involving diagnostics of rotary microsystems) included a patient­evaluation with the help of MKBD­S questionnaire.[20] After diagnosing the complex defects of acupuncture system and the treatment (active points, microsystems, meridian blockages and active scars), a  control TST was made to verify the effects of therapeutic intervention [16]. IOP measurement was made by applanation method with the help of a mobile tonometer – TONOPEN XL. Every measuring is signalized acoustically and visually, average value of IOP (from 4–10 data) is shown on the display. Validity of measuring is also marked (percentage difference of maximal and minimal value: 5–20  %).[5] Tonometer was regularly calibrated and the conditions of measuring were identical before and after the acupuncture treatment: measuring in instillation anaesthetization or without anaesthesia, in the same position (lying or sitting position) and in 15–45 minutes of time range. For comparison of average values of IOP, measured before and after the treatment, a pair T­test was used.

ResultsValues of IOP  RE, LE  before and after the acupuncture treatment are shown in graph  2 and 3.

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Clinic and ResearchValues of Intraocular Pressure and Acupuncture – Pilot Study

Magdaléna Miklósová, M. D.

IOP

Patients no. 1 to 40

Legend:

decr. in IOP incr. in IOP without change

Graph 2 Values of IOP – intraocular pressure (in mmHg) of the right eye before and after acupuncture treatment (green = decrease in IOP, red = increase in IOP, yellow = without changes).

Patients no. 1 to 40

IOP

Legend:

decr. in IOP incr. in IOP without change

Graph 3 Values of IOP – intraocular pressure (in mmHg) of the left eye before and after acupuncture treatment (green = decrease in IOP, red = increase in IOP, yellow = without changes).

Average value of IOP  – RE  (LE) before the acupuncture treatment was  16.5  mmHg; SD 2.6 mmHg (16.3 mmHg; SD 3.3 mmHg) with maximal value of IOP 22.0 mmHg (22.0 mmHg)

and minimal value 9 mmHg (6 mmHg). Average value of IOP RE (LE) after the acupuncture treatment was  15.1  mmHg; SD  1.9  mmHg (14.0 mmHg; SD 2.7 mmHg) with maximal value

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Magdaléna Miklósová, M. D.

of IOP  19.0  mmHg (21.0  mmHg) and  minimal value  14.0  mmHg (8  mmHg). In  the group, IOP of RE decreased by 8.5 %, IOP of LE by 14 % and together by 11.4 % (tab. 1).

Tab. 1 Average values of IOP (intraocular pressure), standard deviation (SD) before and after acupuncture and percentage difference (=decrease) in IOP of the right (RE) and left eye (LE).

Before acupuncture After acupuncture

IOP mmHg average SD average SD difference

RE 16.5 2.6 15.1 1.9 8.5 %

LE 16.3 3.3 14.0 2.7 14.0 %

Statistical analysis:

Right eye: pair t‑test = 3.685 df = 39 P < 0.01

Left eye: pair t‑test = 4.755 df = 39 P < 0.01

Decrease of IOP was in 52 cases (65 %), increase in 9 cases (11 %) and  in case of 19 eyes (24 %) no change of IOP was detected before and after the acupuncture treatment (graph 4).

decrease: 52 eyesincrease: 9 eyes

without change: 19 eyes

24 %

11 % 65 %

Graph 4 Dynamics of IOP – intraocular pressure in the studied group.

In  the group, consensual difference of IOP before and after the acupuncture treatment was also monitored. Average difference of IOP between RE and LE was  2.0  mmHg before and 1.6 mmHg after the acupuncture treatment (20 % decrease). Maximal consensual difference of IOP was 6  mmHg, minimal 0  mmHg. The  whole extension  0–6  mmHg was divided into 4  subgroups: max. difference  5–6  mmHg (=  nonphysiological, sub­standard), medium

difference  3–4  mmHg, minimal difference 1–2  mmHg and no difference. Number of patients with the listed difference of IOP between RE and LE and its percentage arrangement before and after the acupuncture treatment is shown on graph  5. The  biggest decrease of number of patients was in subgroup with maximal difference of IOP between RE and LE (66 % decrease).

difference in IOP before acupuncture after acupuncture

num

ber o

f pat

ient

s

Graph 5 Graph 5 Difference in IOP – intraocular pressure in mmHg between the right and left eye before and after acupuncture treatment.

DiscussionThe  main goal of treatment of glaucoma is to reduce and stabilize the IOP. Within the complex healthcare of the patient with higher or fluctuating value of IOP (or without glaucoma) acupuncture plays an important role apart of the standard treatment. While the other fields of medicine (including ophthalmology) search the description in morphological biochemical and psycho regulative aspect, acupuncture and other natural fields of medicine analyse the energy­informative level. According to the knowledge of the analyser partially also the spiritual aspect can be observed. The real acupuncture requires deep knowledge of its theory and not only in the roots of Traditional Chinese Medicine (TCM), detailed examination in excess of classical recommendations of TCM,

Acupuncture and Natural Medicine 5–6/2015 39

Clinic and ResearchValues of Intraocular Pressure and Acupuncture – Pilot Study

Magdaléna Miklósová, M. D.

individual approach to each patient and evolution of all medical, energy­informative, psychological, social, environmental and spiritual contexts.[15]

Acupuncture determines the human body as a system, which is functional if the single levels and subsystems communicate – cooperate.[13] In case of defect or disease, after a systematic multilevel analysis and localization of the primary defect of the system, it  is possible to approach treatment with usage of the least number of active points.[15] Acupuncture does not treat only the active point, but  the way of interaction between the systems. The  acupuncture point intervenes this interaction.[13] The communication nature of the therapy in the system is oriented anentropically and it fulfills the characteristics of mathematical square principle.[13, 14] The result depends on the aim and concept of interaction, which is defined by the therapist.[13]

Short­term effect of acupuncture on IOP for healthy individuals is introduced in the study of Meira­Freitas D. and col.[12] Authors compare values of IOP in the group of 48 patients (94 eyes). They divided them into 3 subgroups: the control group of 15  patients without intervention, the second group of 14 patients with application of acupuncture into less active points resp. “placebo acupuncture” and the third group of 19  patients with using the same acupuncture points (GB1, GB14, PL1). Their IOP was tested by Goldmann tonometry 20  min. before and 24  hours after acupuncture. The  conclusion of the study does not show a significant difference of measured IOP between these groups. Despite of the statistic conclusion of this study the final test results suggest significant changes in IOP after applied acupuncture comparing with the control group. Nowadays we can still find the term of “placebo acupuncture”, which in point of latest cognition of micro system and projections of each region of the body is fully irrelevant.

[17] If  we respect the organism’s acupunctural system, we  can not adjust the acupunctural treatment to western diagnostic units.[15] Another study compared IOP before and 5 minutes after treatment of one acupuncture point, in a group of 40  healthy individuals (79  eyes). In  this study Liu  Y. & co.[11] report results similar to our study. Authors achieved a decrease in IOP in 49, an increase in 8 eyes, and in 22 eyes there was no change in IOP after acupuncture. At the conclusion of the study they report a significant decrease in average IOP by 1,61mmHg. Law SK. & co.[10] report about the results of a prospective and randomized study of 11  patients with primary open­angle glaucoma and stable  IOP. The  authors found a slight increase in average values of IOP after acupuncture treatment of points “eye­related” (from  12,9mmHg to 13,6mmHg) and  of points “non­eye­related” (from 13,0mmHg to  13,5mmHg). Modern acu­punctural diagnostics  e. g., like TST or EAG do  not confirm points related or non­related to the eye, nor  they do not it confirm so called distant or near points.[14, 18].

Many studies confirm the effect and security of acupuncture during the treatment of glaucoma.[2,  7,  9,  21] The  effect of acupuncture of 18 patients with glaucoma or hypertension of the eye is published in prospective study of Uhrig S. a  col.[21] German authors observed significant decrease of IOP in average of 2.5 mmHg, which was measured 15 minutes before, also 15 minutes and 24 hours after the standardized acupuncture. In  another prospective and randomized study Her  J.­S. & co.[7] confirmed significant decrease of IOP in a group of glaucomatics after auricular acupression in comparison with a group without acupression. The  most significant decrease in IOP was found during the 3rd to 4th week after auriculotherapy.

Acupuncture and Natural Medicine 5–6/2015 40

Clinic and ResearchValues of Intraocular Pressure and Acupuncture – Pilot Study

Magdaléna Miklósová, M. D.

In  our pilot study, we  evaluated the effect of a complex acupuncture treatment on IOP and we examined the nature of its effect on values of IOP of patients without any acute eye disease and glaucoma. We  recognized that after the acupuncture intervention the values of IOP in most of the cases decreased. Increase of IOP (but only in extent of physiological values) after the treatment we recognized in cases of relative hypotonia and unbalanced values of IOP between RE and  LE. We  proved regulative effect of acupuncture on IOP – according to the position of energy­interactive system it decreases, in case of relative hypotonia it increases and balances the values of IOP between RE and LE. The result of the energy­informative stabilization is a general harmonization of the patient including IOP and improvement of eye functions.

Complex impact of acupuncture can be un­derstood if we will proceed from the theory of structural fields – including gnostic field. This fact results in the idea that material manifestations are secondary, e.g. they are the results of operations of these fields.[19]

SummaryThe results of the examined group confirmed the change of IOP after an acupunctural treatment in 76 % of cases (decreased 65 %, increased 11 %).The  pilot study proved, that acupuncture has statistically significant effect of IOP (P  <  0.01) and  its effect on IOP is of regulative nature (it decreases the risk of formation and progression of glaucoma, it also enables the right functioning of the eye).

In 24 % of cases, where no changes of IOP were found after acupuncture, we assume the effect of other factors which evoke that the change does not show or it shows later.

Regulative and fast effect of acupuncture on IOP can be explained by these processes being energy­informative. The character of this regulative effect on IOP needs to be studied further, through complex analysis of the patient.

Thanks toI  would like to thank to  G. Solár, M. D., PhD. and  PaeDr. Z. Solárová, PhD. for  their help and cooperation while doing acupuncture examination and treatment in clinic and for their valuable professional advice also to  Mgr. P. Miklós, PhD. (from PF UK in Bratislava) for statistical processing of results of the study.

Magdaléna Miklósová, M. D.NZZ – eye clinicHodská 3160/101, 924 01 Galanta, Slovak republicE-mail: [email protected]

References1. Ando, V.: Klasická čínska medicína – základy

teorie I. Hradec Králové, Svítaní, 1995, s. 205 – 217.

2. Astbury, N.: Alternative eye care. British Journal of Ophthalmology, 2001, 85: 767–768, [online] http://bjo.bmj.com/content/85/7/767.full [11. 10. 2015].

3. European Glaucoma Society: Terminology and Guidelines for Glaucoma. II. Edition, Dogma, Italy, 2003, ISBN 88­87434­13­1, Ch. 1: 3–4, [online] <http://www.oogheelkunde.org/uploads/4D/w6/4Dw6l> pdf/39­40pdf [4. 9. 2015].

4. Ferková, S.: Nefarmakologická liečba glaukómu. Bulletin Slovenskej glaukómovej spoločnosti vydávaný pri príležitosti Svetového týždňa glaukómu, 2012, s. 12, ISBN 978­80­970870­0­5.

5. Filouš, A., Burdová, M., Malec, J.: Tono­pen XL – tonometr vhodný pro standardní i meně obvyklé podmínky při měření nitroočního tlaku. Čs. Oftal., 54, 1998, No. 3, s. 159–165.

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Clinic and ResearchValues of Intraocular Pressure and Acupuncture – Pilot Study

Magdaléna Miklósová, M. D.

6. Gerinec, A.: Glaukómy u detí. Martin, Osveta, 2002, s. 60 – 61, ISBN 80­8063­103­4.

7. Her, J. S., Liu, P. L., Cheng, N. Ch. et al.: Intraocular Pressure­Lowering Effect of Aurical Acupressure in Patients with Glaucoma: A Prospective, Single­Blinded, Randomized Controlled Trial. The Journal of Alternative and Complementary Medicine, 2010, 16 (11): 1177–1184, [online] http://online.liebertpub.com/doi/abs/10.1089/acm.2010.0020 [11. 10. 2015].

8. Kuchynka, P. a kol.: Oční lékařství. Praha, Grada, 2007, s. 571, ISBN 978­80­247­1163­8.

9. Law, S. K., Li, T.: Acupuncture for glaucoma. Cochrane Database of Systematic Reviews, 2013, Issue 5, [online] <http://onlinelibrary.wiley.com/doi/10.1002/14651858.CD006030.pub3> [20. 11. 2015].

10. Law, SK., Lowe, S., Law, SM., et al.: Prospective Evaluation of Acupuncture as Treatment for Glaucoma. American Journal of Ophthalmology, 2015, Volume 160, Issue2, p. 256–265, [online] http://dx.doi/10.1016/j.ajo.2015.04.033 [11. 10. 2015]

11. Liu, Y., Long, YS., Long, YS.: The immediate effects of acupuncture on intraocular pressure. Chinese Acupuncture & Moxibustion, 1994, 14, 41.

12. Meira-Freitas, D., Cariello, A. J., Vita, R. C. et al.: Short­Term Effect of Acupuncture on Intraocular Pressure in Healthy Subjects. Acupunct Med, 2010, 28/1: 25–7, Department of Ophthalmology, Federal University of Sao Paulo, Brazil, [online] <http://www.academia.edu/3579022> [11. 10. 2015].

13. Mochnáč, T.: Acupuncture and System’s Interactions. In Acupuncture and Natural Medicine, 2013, 2, p. 27 – 30, MSNM, Bratislava, ISSN 1339­4703.

14. Slanina, F.: Teorie sítí: společný jazyk buňky a internetu. [online] <http://archiv.otevrena veda.cz/users/Image/default/C1Kurzy/NH2006>, pdf/6­7.pdf [3. 12. 2013].

15. Solár, G.: Acupuncture and Natural Medicine. In Acupuncture and Natural Medicine, 2013, 2, p. 5 – 12, MSNM, Bratislava, ISSN 1339­4703.

16. Solár, G.: Niektoré mechanizmy akupunktúry a TST. In Acupuncture and Natural Medicine, 2014, 1, s. 5 – 12, MSNM, Bratislava, ISSN 1339­4703.

17. Solár, G.: Tripletsa, Concatenation, Bagua and Patterns in Acupuncture. In Acupuncture and Natural Medicine, 2014, 2, p. 5 – 14, MSNM, Bratislava, ISSN 1339­4703.

18. Solár, G.: Energy­Informative Networks in Acupuncture. In Acupuncture and Natural Medicine, 2014, 3, p. 9 – 13, MSNM, Bratislava, ISSN 1339­4703.

19. Solár, G., Solárová, Z.: The Complex Dynamic Pyramid Model in Acupuncture. In Acupuncture and Natural Medicine, 2014, 5, p. 8 – 15, MSNM, Bratislava, ISSN 1339­4703.

20. Solárová, Z.: Diagnostic Possibilities and Perspectives of a Modified MKBD­S Questonnaire According to Korngold and Beinfield. In Acupuncture and Natural Medicine, 2013, 1, p. 22 – 26, MSNM, Bratislava, ISBN 978­80­970500­3­0.

21. Uhrig, S., Hummelsberger, J., Brinkhaus, B.: Standardized acupuncture therapy in patients with ocular hypertension or glaucoma – results of a prospective observation study. Bugenärztliche Praxis, Wiesbaden, Deutschland. Forsch Komplementarmed Klass Naturheilkd, 2003/Oct.10, (5): 256–61, [online] <http://www.ncbi.nlm.nih.gov/pubmed/14605482> [10. 10. 2015].

Acupuncture and Natural Medicine 5–6/2015 42

Interdisciplinary InsightsThermal Effects in Electro Acupuncture

Assoc. Prof., Dipl. Ing. Branislav Hučko, PhD., Ing. František Horvát, Ing. Tomáš Kováč, Ing. Lukáš Šoltés, Ing. Michal Čekan, PhD.

Thermal Effects in Electro Acupuncture

Assoc. Prof., Dipl. Ing. Branislav Hučko, PhD., Ing. František Horvát, Ing. Tomáš Kováč, Ing. Lukáš Šoltés, Ing. Michal Čekan, PhD.

SummaryElectro acupuncture (EA), and  its physical effects, are currently still rather underdeveloped in a scientific point of view, despite its widespread practice in natural medicine. There is a need however to review the current scientific state of  EA.[1,  7] It  is therefore necessary to expand scientific study within this field to include and investigate the physical effects as well. In  terms of the thermal effects in EA practise, the article investigates the thermal effects around the needle and determine what mechanisms may cause some common complaints during acupunctural treatment, such as feelings of itchiness and burning at the location of the acupuncture needle. The  problem is represented by finite element analysis (FEA)[2,  3] concentrating on a basic thermal analysis described by nominal physical and thermal properties of body tissues in vivo.[4, 5] The  model is created in the Ansys Workbench FEA environment. The  model considers the thermal effects of an EA needle and its resulting effect on the surrounding tissue.

Key WordsElectro acupuncture, thermal effects, Ansys Workbench, isotherms, tissue heat transfer

IntroductionElectro acupuncture is a diagnostic and therapeutic method of alternative medicine that combines traditional Asian teachings and electrical effects to create a modern form of the practice. The needles are subject to electrical signals of varying character to

stimulate the energy paths through each meridian in patients, fig. 1. Transfer of the signal through each meridian is performed by using a generator which transmits electrical signals from  100 to 1,000  mV with frequencies ranging from  10 to 80 Hz.[6] An important aspect to consider in such a procedure is the subjective description of the patients feelings at the acupunctural point being observed, primarily at the interface between the needle and surrounding tissue. Any  heat generated may be described proportionally by the impedance in the skin/tissue and voltage of the generated signal. Any heat generated within the needle can then be simulated, where heat distribution in the tissue around the needle can be observed/described.

MethodologyIn  order to analyse the thermal effects that the EA needle has it is not only necessary to know the physical properties of affected human tissues, such as density, isotropic heat conductivity or specific heat, but also to determine the appropriate boundary conditions used within the analysis. These conditions are dictated by known properties such as the temperature of the body 36.5 °C and maximum interfacing temperature, 60 °C, which may cause proteins to clot therefore resulting in damage to the tissue. It is also necessary to know

Acupuncture and Natural Medicine 5–6/2015 43

Interdisciplinary InsightsThermal Effects in Electro Acupuncture

Assoc. Prof., Dipl. Ing. Branislav Hučko, PhD., Ing. František Horvát, Ing. Tomáš Kováč, Ing. Lukáš Šoltés, Ing. Michal Čekan, PhD.

the thermal properties of the needle material being used in EA and the properties of the tissues in which the needle is to be inserted, depth of insertion and geometric properties of the needle – tab. 1. The presented analysis assumed a needle made of medical grade stainless steel with a diameter of  0.15  mm and an insertion depth of 10mm.[6] The  model was analyzed in the ANSYS Workbench FEA environment and is setup to investigate the distribution of thermal gradient by discretizing the model into a finite number of elements (model meshing). Such a model can then be solved and the thermal effects for each individual element can be determined. The  model was simplified into a 2D symmetric problem fig. 2.

Fig. 1 Example of common acupuncture points on the human body

Tab. 1 Properties of EA needle and tissues used in the simulation

Element Dimensions[mm]

Density[kg/m³]

Thermal Conductivity

[W/m.°C]

Specific Heat[J/kg.°C]

Needle 0.15 × 15 8,055 13.8 480Skin 15 × 2 1,109 0.37 3,391Subcutaneous Fat 15 × 2 911 0.21 2,348Muscle 15 × 11 1,090 0.49 3,421

The model consists of a simplified representation of tissues that may be affected by the EA needle. They are represented by three layers: skin, subcutaneous fat and muscle seen in  fig. 2. Dimension of each layer can be seen in  Tab.  1 for which each represent a generalized mean value for variances throughout the human body. The  table also contains thermal properties for each part represented in the analysis. Each layers interface was defined appropriately, where the interface between the skin, subcutaneous  fat, and muscle are represented as conductive, while the interface between the skin and air was defined

by free convection (4.8 W.m−2.°C−1).[5] The lower portion of the model was defined to be at body temperature, 36.5 °C.The analysis results were defined to determine the boundary of nodes with the same temperature (isotherm). The isotherms were then plotted on the model. It  was also necessary to investigate the way each isotherm was effected due to temperature variations in the needle, therefore the needle in the model is subject to three different temperatures: 40, 50 and 60  °C. This change in needle input temperature and its corresponding effect on the surrounding tissues were then plotted and compared to each other in order to determine any correlations.

Acupuncture and Natural Medicine 5–6/2015 44

Interdisciplinary InsightsThermal Effects in Electro Acupuncture

Assoc. Prof., Dipl. Ing. Branislav Hučko, PhD., Ing. František Horvát, Ing. Tomáš Kováč, Ing. Lukáš Šoltés, Ing. Michal Čekan, PhD.

SkinSubcutaneous Fat

MuscleEA needle

Fig. 2 FEA model of the different layers of tissue as well as EA needle

ResultsIn Fig. 3 the thermal gradient (isotherms) can be seen for different input temperatures of the needle. It is logical that the number of isotherms increased, as well as the affected area, depending on the input temperature of the needle. At 40 °C

three isotherms were registered, at  50  °C the number of isotherms increased to 9 were at 60 °C a total of 14 isotherms were generated.

The projection of the isotherms horizontally and vertically into the surrounding tissue from the needle were recorded for each input temperature. These “diameters” were plotted and compared in terms of their dependence on input temperature. The  graph in  fig.  4 shows the dependence of horizontal isotherm distance from the source of heat as the temperature of the source (EA needle) increased. The  same effect can be observed in fig. 5 for vertical distance of the isotherms from their source. From these two dependencies it is quite obvious that the corresponding distance of the isotherms grew rapidly as the temperature of the source was increased.

a)

Acupuncture and Natural Medicine 5–6/2015 45

Interdisciplinary InsightsThermal Effects in Electro Acupuncture

Assoc. Prof., Dipl. Ing. Branislav Hučko, PhD., Ing. František Horvát, Ing. Tomáš Kováč, Ing. Lukáš Šoltés, Ing. Michal Čekan, PhD.

b)

c)

Fig. 3 Isotherms for different input temperature of the EA needle: a) 40 °C b) 50 °C and c) 60 °C

Acupuncture and Natural Medicine 5–6/2015 46

Interdisciplinary InsightsThermal Effects in Electro Acupuncture

Assoc. Prof., Dipl. Ing. Branislav Hučko, PhD., Ing. František Horvát, Ing. Tomáš Kováč, Ing. Lukáš Šoltés, Ing. Michal Čekan, PhD.

Isotherms: X distance

dist

ance

temperature

Fig. 4 Isotherm diameters from heat source (horizontal)

The results of the analysis support the assumptions that define the relationship between EA needle temperature and isothermal behavior in the analyzed mediums. Furthermore the isothermal

shapes display moderately different behaviors through the thickness of each medium, most notably between the muscle, subcutaneous fat and skin (see fig. 3).

Isotherms: Y distance

dist

ance

temperature

Fig. 5 Isotherm depth (distance) from heat source

Acupuncture and Natural Medicine 5–6/2015 47

Interdisciplinary InsightsThermal Effects in Electro Acupuncture

Assoc. Prof., Dipl. Ing. Branislav Hučko, PhD., Ing. František Horvát, Ing. Tomáš Kováč, Ing. Lukáš Šoltés, Ing. Michal Čekan, PhD.

DiscussionThe  presented analysis represents a simplified representation of the human body. It served as a pilot analysis specifically intended to investigate, at a basic level, the heat effects that an EA needle may have on the surrounding tissues. The model was created using linearized, homogeneous and isotropic assumptions for the definition of tissue and the EA needle, an assumption that may not be valid through some of the mediums used throughout the analysis. It  will be necessary to develop more complex material models to more accurately determine the behaviour of isotherms throughout each tissue. The  article assumes temperatures of the EA needle, however it must be understood that these do not represent actual temperatures that may occur within the needle. For  this it will be necessary to perform experiments and determine input voltage, frequency, impedance, needle locations, etc… to determine these values. Once obtained, a  more complex model may be created that will more accurately describe the thermal effects occurring throughout the body.

The  article does not take into consideration more complex thermal effects nor the changes that these effects have on the physiological or psychological state of the human body. For  this it will be necessary to work with acupuncturists and compare data with their subjective diagnosis in order to find some correlation between some physical quantity and a specific subjective feeling that a patient may have.

Regardless the presented study was successful in identifying some basic thermal behaviours which occur throughout human tissue, that being:1. With increased source temperature, the isotherm

boundaries grew rapidly from the source in both the vertical and horizontal directions.

2. Isotherm behavior is effected by thermal properties of the tissues.

In  terms of any feeling of discomfort, itchiness, burning at the location of the EA needle inserted into the human body, the  heat affected zone grows rapidly as temperature of the EA needle increases. This rapid growth can affect nearby nerve endings, and  depending on the intensity of heat, any  feelings of discomfort may grow exponentially as heat build within the EA needle. This of course will also require experiments to verify.

It was also obvious that in the even the EA needle reaches a higher temperature, the  heat effected zone grew from  4.79  mm at 50  °C to  8.56  mm at 60  °C. it  will also be necessary to perform a transient thermal analysis in future works in order to determine changes over time.

ConclusionsThe presented analysis was intended to determine a basic (linear and isotropic) but  fundamental description of the thermal effects occurring in human tissue as a result of electro acupuncture. The focus was to investigate the thermal behavior of tissue when subject to varying temperatures of the EA needle. It  was determined that with growing needle temperature, a  rapid increase in heat effected zone were observed (see fig. 3). It  was determined that over a 10  °C increase in needle temperature, the  heat effected zone doubled in size (both horizontally and vertically), see fig. 4 and 5. Isotherms also rapidly increased throughout the volume of this zone over such a change in temperature. Therefore it can be concluded that a small increase in needle temperature can have a meaningful impact on the thermal effects throughout the human body, which may lead to feelings of discomfort, such as itchiness or burning. However there were no experimental results for which the analysis could be compared, therefore future studies must contain a reliable and repeatable experiment that can be used to create a more complex and therefore

Acupuncture and Natural Medicine 5–6/2015 48

Interdisciplinary InsightsThermal Effects in Electro Acupuncture

Assoc. Prof., Dipl. Ing. Branislav Hučko, PhD., Ing. František Horvát, Ing. Tomáš Kováč, Ing. Lukáš Šoltés, Ing. Michal Čekan, PhD.

accurate description/analysis of the thermal effects and perhaps determine some correlation between these effects and the subjective feelings in patients who undergo EA.

Assoc. Prof., Dipl. Ing. Branislav Hučko, PhD., Ing. František Horvát, Ing. Tomáš Kováč, Ing. Lukáš Šoltés, Ing. Michal Čekan, PhD.Slovak university of technology in BratislavaFaculty of mechanical engineeringNámestie slobody 17, 812 31 Bratislava 1, Slovak republicE-mail: [email protected]

References1. Andrew C., A., Colbert, A. P., Anderson, B. J.,

Martinsen, Ø. G., Hammerschlag, R., Cina, S., Wayne, P. M., Langevin, H. M.: Electrical Properties of Acupuncture Points and Meridians: A Systematic Review. Wiley InterScience, 2008.

2. Benča, Š.: Výpočtové postupy MKP. Vydavateľstvo STU, Bratislava, 2006, ISBN 80­227­2404­1.

3. Jančo, R.: MKP v riešení nosníkov a rámov na pružnom podklade. 1. vyd., Bratislava: Nakladateľstvo STU, 2013, s. 109, ISBN 80­227­3880­4

4. Sheng L., Pan, J., Zhou M. C.: A Semi­quantitative Method to Study Electrical Properties of Acupuncture Points. International journal of intelligent control and systems, 2008.

5. de Dear R. J.,•Arens, E., Oguro, Z. H. M.: Convective and radiative heat transfer coefficients for individual human body segments. Int J. Biometeorol, 1997.

6. Hasgall P. A., Di Gennaro, F., Baumgartner, C., Neufeld, E., Gosselin, M. C., Payne, D., Klingenböck, A., Kuster, N.: IT’IS Database for thermal and electromagnetic parameters of biological tissues. Version 3.0, September 01st, 2015, DOI: 10.13099/VIP21000­03­0. www.itis.ethz.ch/database

7. Teplan, M.: Akupunktúra – princípy, prístupy a perspektívy výskumu. ICMART XIII World Congress, 2008.

Acupuncture and Natural Medicine 5–6/2015 49

Interdisciplinary InsightsSlope Movements as a Geofactors of the Environment and their Impact on Human

Prof. Dr. František Baliak, PhD., Mgr. Martin Brček, PhD.

Slope Movements as a Geofactors of the Environment and their Impact on HumanProf. Dr. František Baliak, PhD., Mgr. Martin Brček, PhD.

SummarySlope movements belong to the most significant geodynamic phenomena, which strongly influence the geological nature as a part of the environment which man used and modified.

The  basic components of the geological nature include geological factors. These factors influence the use of environment including humans. Geofactors are divided into two groups: geopotentials (resources and possibilities of geological nature) and  geobarriers (various obstacles and limitations of geological nature).

This article gives examples of slope movements which have a positive effect on man as geopotential  – significantly contributes to im­prove the quality of life, e.g. stable slopes; and  also examples of geobarriers, which have a negative effect on human (factors threatening the life and work of  man, factors inducing adverse interaction in the geological environment and thus significantly reduce the efficiency, durability and safe operation of technical works and factors constituting retrospective negative effects of technical works that seriously damage geological environment.

Key Wordsslope movements, slope deformation, en­vironment, geological environment, geopotential, geobarriers, human life

IntroductionThis article follows the report, which was presented at the XIX. Medical Congress of Natural Medicine in Nitra in 2015. The topic is a continuation of the issue of the impact of environmental geofactors to humans, which we presented in previous issues of this magazine (Baliak  & Khun, 2012; Baliak, Ondrášik, Brček, 2014 and Baliak, Ondrášik, Brček, 2015). In the paper Baliak & Khun, (2012), we  have briefly described all geological hazards of the environment in general, and  we have shown their impact on humans. In the paper by Baliak, Ondrášik, Brček (2014), we discussed in depth geofactors focusing on water. Also in the paper by Baliak & Brček (2015), we  focused on rock environment as a basic component of the geological environment. This paper is devoted to the slope movements and their effect on humans.

An Overview of Geological Factors of the EnvironmentGeofactors divided into geopotentials and geobarriers (Matula, 1995). Geopotentials include various natural resources and potential usable of the geological environment factors supporting human development e. g. raw  materials, stable slopes, good quality agricultural and foundation soils, healing and drinking water. Geobarriers represent various obstacles and limitation of the natural­geological environment factors thus jeopardizing life and human activities ­engi­neering works (volcanic eruptions, earthquakes, disastrous slope failures, flooding, etc.), factors decreasing efficiency of construction and operation of engineering works (foundation soils

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Interdisciplinary InsightsSlope Movements as a Geofactors of the Environment and their Impact on Human

Prof. Dr. František Baliak, PhD., Mgr. Martin Brček, PhD.

with high compressibility, slopes with low stability) and  factors deteriorating the environment due to negative anthropogenic activity (settlement of the terrain due to underground mining, high groundwater level and waterlogged foundation soil, unloaded slope  toe, etc.). This means that geo­potential increases the quality of human life and vice versa geo­barriers reduce it.

We  have detailed overview of geological en­vironmental factors published in issue 1/2015 (Baliak, Ondrášik, Brček, 2015).

Slope Movements as a Geofactor of EnvironmentLandslides are significant geodynamic phenomena, which strongly influence the geological environment as a part of the environment that man uses and changes.

Slope movements are gravitational movements of rocks along the slope. This kind of movement is different from the transport of rocks along the slopes caused by transport media – by snow, ice  and wind. The  slope movements result in slope deformations – slope failures.

The development of slope movements is caused by certain natural conditions, by climatic, hydro­geologic and geomorphologic circumstances, which are the basic preconditions for development of the movement. They either support the movement or they prevent  it. Thus, some conditions are either favourable, or unfavourable for development of slope movements. These conditions influencing factors of slope movements may by defined as processes of changes of given conditions. Each slope has a certain degree of slope stability larger than 1. Its variation in time

is caused by some of the factors. Factor, which is the most important in the moment of slope movement inception, is called the trigger of the slope movement.

Classification of slope movements is divided into four groups, according to Nemčok, Pašek, Rybář (1974):1. creep2. slide3. flow4. fall

CreepThe slope creep movement is a long­term, slow movement of rock masses, their boundary being usually indistinct due to the solid bedrock. The extent of the mass moving is negligible with regard to the space dimensions of the concerned rock massive. Therefore, the  resulting forms of creeping are usually not outstanding – occurring as gravitationally torn and broken ridges and slopes. Hard complexes deposited on soft subsoil, which due to creeping gets broken to usually one millimetre to one centimetre per 10 years. If this process is enhanced creeping is transformed into sliding, flowing or falling. Creeping is a preparatory phase to other forms of slope movements.

SlidingSliding is a relatively rapid, short­term movement of rock masses along one or several shear planes, diving (separating) the moving masses from the solid subsoil. Resulting form – the  slope failure developed by sliding are landslides. The landslide may be classified according to the shape of the shear plane, surface shape and activity.

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Prof. Dr. František Baliak, PhD., Mgr. Martin Brček, PhD.

According to the shape of the shear plane we distinguish:

a. Rotational landslides, having the shear plane shape of a cylinder (Fig. 1a); develop in homogenous clayey soils.

b. Planar landslides, their shear plane is predetermined (Fig.  1b, 1c), using the interface between the subsoil and cover formations, interlayer surfaces or tectonic planes.

c. Rotational-planar landslides, having com­posite (sometimes irregular) shear planes (Fig. 1d).

d. Translation landslides (Fig.  1e) occurring on the horizontally developed shear plane.

Fig. 1 Types of landslides

According to the areal shape we distinguish:a. Areal landslides, having approximately

the same length and width. They develop on flat slopes, having usually smaller dimensions and depth only several meters, exceptionally 10 m.

b. Stream-like landslides have elongated shape (the  length exceeding several times the width). They are often several kilometres long and 30 to 40 meters thick. The most serious economic damages were caused by the stream­like landslides; for example the stream­like landslides at Handlová, which happened in  1960 destroyed more than 150 houses; it was 1,800 m long, the volume

of sliding masses exceeded 20 mil. m³ and maximum motion velocity was  6.3  m per day.

c. Frontal landslides. In  the case of frontal landslides the width exceeds the length. They develop usually on the river banks due to lateral erosion caused by wave shocks.

According to the degree of activity we distinguish:a. Active landslides. The  surface of active

landslide is rather broken up (disrupted) demonstrating recent movements. The movement is periodically activated by natural factors.

b. Potential (calm-stabilized) landslides, with uneven surface, without recent shapes. They may be activated by intense natural factors (erosion, precipitation).

c. Dormant – stabilized landslides. The  land­slide surface is usually levelled, thus it cannot be demarcated, and  identification is difficult and possible only by considering the general slope shape. Activation may occur due to anthropogenic factors.

FlowingFlowing is a rapid, short­term movement (m.h−1, km.h−1) of rock materials in viscose state. The  flowing mass is sharply separated from the undisturbed subsoil.

The most frequent resulting forms of flowing are earth flows and stone flows.

a. Earth flows are developed in places of concentrated flow of surface and groud­waters (Fig.  2a). As  example may be mentioned the earth flow, which in  1962 caused a disaster of village Lieskové and Riečnica nad Kysucou, when 29  houses were destroyed.

b. Debris flows develop on steep slopes of high mountains. Products of weathering accumulate in glens. Storm waters drag

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Interdisciplinary InsightsSlope Movements as a Geofactors of the Environment and their Impact on Human

Prof. Dr. František Baliak, PhD., Mgr. Martin Brček, PhD.

down stony and sandy­clay material with a velocity attaining several tenth of km.h−1 and transport them down into valleys, where debris consensually develops (Fig. 2).

Fig. 2 Basic types of slope failures of flowing

FallingFalling is a rapid, abrupt short­term movement (m.s−1) of  rock masses on steep slopes, when at least a part of the movement occurs as free fall.

Basic types of slope failures of the group of falling are rock toppling and planar rock falls.

a. Toppling occurs when rock materials on steep rocky walls or overhangs fall away as free falls (Fig. 3a).

b. Planar rock falls (Fig.  3b) are  abrupt movement’s masses of rock along the plane slip surface that become detached from steep slopes or cliffs and movement occurs by free­fall.

Fig. 3 Basic types of slope failures of falling

Slope failures represent a significant factor in geological hazard, jeopardizing the environment of Slovakia. Up  to the present time, regional

investigation carried out in Slovakia revealed 21,190 slopes failures (mostly landslides), which occupy an area of 257,591.2 hectares, it is 2.5 % of the total area of Slovakian territory (Baliak, Ondrášik, Brček, 2015).

Effect of Landslides on HumanAs  indicated slope movements influence man either as geopotential or geobarrier.

Landslides as a GeopotentialsSlope movements and their resulting spatial forms as a geopotentials have generally positive influence on the quality of human life. It is mainly by the fact, that in the areas of interest (which man uses for his activities) are  also occurring zones that have never been broken by landslides or which were successfully stabilized by men with the various remedial actions that were proposed by the engineering­geological investigation.

These areas: – allow the smooth use of the area for agricultural

purposes to ensure human nutrition, – provide plenty areas for the construction of

any kind, – increase the aesthetics of landscapes.

Landslides as a geobariersLandslides and the resulting deformation as a geobariers generally impose negative impacts on quality of human life because:

– they the jeopardize life and works of  man, including catastrophic landslides, rock falls, toppling falls, rock shedding (bouncing and rolling), debris flow, earthflow and mudflow.

Many of these movements (deformations) are  catastrophic with direct consequences for man and his work (Tab. 1).

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Prof. Dr. František Baliak, PhD., Mgr. Martin Brček, PhD.

Tab. 1 Summary table of the disastrous slope movements in the years 1903 to 2004 on various continents (Ondrášik, Vlčko, Fendeková, 2011). The table did not include data from China in 1920.

Continent Event count Victims Overall stricken population

Economic damage (in million U.S.

dollars)Africa 22 721 19,740 0

America 139 20,532 4,667,943 1,318Asia 220 15,754 5,056 534

Europe 75 16,158 41,536 1,706

Between the most tragic events, e.g. in  landslides in China (Ningxia Haiyuan) in  1920  died  100,000  people; in  1980  a major volcanic eruption occurred at Mount St. Helens caused landslides and total of 57 people lost their lives. In the Europe, it was a landslide and flood at Vajont Dam, on the Vajont River in the Italian Alps where nearly 3,000 people died (Fig. 4).

Fig. 4 Head scrap of Vajont landslide in Italy, 1963

Fortunately, in  Slovakia we have not recorded any loss of life directly linked to formation of slope movements. In some cases, resulting slope deformations triggered in humans, the  amount

of stressful situations that after some time had a significant effect on mortality. However, in  our country slope movements significantly jeopardize the work of man. For example, disastrous landslide in Handlová (1960–1961) destroyed 15  houses, 2 km road I/50, water supply, VHV mast (Very High Voltage) and others (Fig. 5).

Fig. 5 House destruction caused by Handlová landslides (1960–1961)

Number of landslides in Slovakia in  2010 reached  577; these landslides damaged 136  houses, roads with total length of 4  km, 0.4  km railway line and others (Baliak, Stríček, 2012), (Fig. 6).

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Interdisciplinary InsightsSlope Movements as a Geofactors of the Environment and their Impact on Human

Prof. Dr. František Baliak, PhD., Mgr. Martin Brček, PhD.

Fig. 6 Destruction of house caused by landslide, Nižná Myšla in 2010Atlas of Stability of Slopes in SR at 1:50000 scale give evidence about the threat to civil engineering works.

Tab. 2 Statistical analysis results of project “Atlas of slope stability maps of Slovakia” (Šimeková, Martinčeková et al., 2006)

Engineering geological region

Regi

on o

f hig

h co

re

mou

ntai

ns (A

a)

Regi

on o

f mid

cor

e m

ount

ain

(Ab)

Regi

on o

f flis

h hi

ghla

nds

(Bc1

)

Regi

on o

f flis

h hi

ghla

nds

(Bc2

)

Regi

on o

f flis

h hi

ghla

nds

(Bc3

)

Regi

on o

f flis

h up

land

s (B

d1)

Regi

on o

f flis

h up

land

s (B

d2)

Regi

on o

f flis

h up

land

s (B

d3)

Regi

on o

f vol

cani

c hi

ghla

nds

(Ce)

Regi

on o

f vol

cani

c up

land

s (C

f)

Obl

asť D

g vn

útro

hors

kotli

ny

Obl

asť D

h vn

útro

karp

atsk

é ní

žiny

Celk

om

Total slope deformations (SD) 1,509 749 1,973 21 442 6,433 2,152 1,943 1,846 515 3,220 387 21,190

Average slope angle (°) 25.5 15.9 16.6 14.1 15.3 13.2 13.4 13.7 13.4 1 1.5 10.1 9.1 14.0

Disturbed area (ha) 17,653.3 6,088.5 20,452.7 289.1 6,786.7 70,167.2 21,620.6 18,853.4 38,566.1 13,648.2 36,347.5 7,118.0 257,591.2

% of the disturbed surface area 4.7 0.7 11.9 11.9 7.7 13.5 15.8 9.3 11.8 7.2 5.2 0.5 5.3

Agricultural land (ha) 2,621.7 3,147.4 6,193.8 170.6 2,657.2 38,114.5 14,232.7 12,528.2 12,331.5 5,970.1 27,232.9 5,089.4 130,289.9

Forest area (ha) 11,267.1 2,832.5 14,080.8 118.5 4,103.7 31,064.4 7,072.8 6,129.6 25,882.3 7,526.7 8,321.4 1,843.6 120,243.2

Other areas (ha) 3,764.6 108.7 178.1 25.8 988.3 315.1 195.6 331.3 152.4 793.3 185.0 7,058.1

Highway s and primary road (m) 3,697.0 300.0 4,630.0 700.0 26,308.0 21,002.0 12,780.0 3,830.0 2,265.0 21,995.0 1,309.0 98,816.0

2nd and 3rdclass road (m) 7,320.0 26,584.0 21,095.0 4,945.0 235,165.0 58,890.0 48,418.0 35,592.0 14,995.0 93,674.0 24,730.0 571,408.0

Railways (m) 3,640.0 5,667.0 4,505.0 7,963.0 10,385.0 3,890.0 7,895.0 315.0 13,100.0 9,850.0 67,210.0

Building constructions (pc.) 401 1,134 3,164 128 9,646 3,224 2,233 2,025 816 4,436 713 27,920

Other constructions (pc.) 21 19 16 9 212 45 54 47 14 135 28 600

Threatened more than 50 objects 7 6 3 3 46 18 10 15 1 49 10 168

Pipes for energetic fluids (m) 26,630.0 31,905.0 79,235.0 11,120.0 364,565.0 116,390.0 91,041.0 95,400.0 22,490.0 238,770.0 38,510.0 1,116 056.0

Gas pipeline (m) 1,435.0 2,716.0 2,110.0 550.0 21,224.0 14,610.0 9,310.0 13,565.0 920.0 30,600.0 4,140.0 101,180.0

Duct (m) 1,300.0 250.0 480.0 1,470.0 3,500.0

Water pipe (m) 8,100.0 1 1,470.0 1 1,040.0 5,600.0 39,905.0 24,880.0 24,220.0 45,630.0 7,560.0 96,850.0 15,670.0 290,925.0

– Slope movements, such as geological factors of the environment also produce negative interaction between geological environment and work of man and thus reduce the efficiency of construction and operation of civil engineering works. Further, slope movements generated enormous economic damage where

remediation takes place. For example, United States of America for these purposes annually spends $ 3.5  billion, Slovakia only about 1 million (Ondrášik, Vlčko, Fendeková (2011).

– Finally, slope movements rank among the geological factors representing the negative effects of technical works that seriously harm

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Prof. Dr. František Baliak, PhD., Mgr. Martin Brček, PhD.

(anthropogenically) geological environment and call for its protection and rehabilitation (Fig. 7, 8).

Fig. 6 Landslide of the road cut – R1 expressway, Nitra in 2010

Fig. 7 Remediated landslide – R1 expressway, Nitra in 2015

ConclusionOverall, it  can be stated that slope movements and their slope deformation as a geofactor of the environment have a significant impact on human life, especially by following:

– they directly threaten the life and work of man; respective, significantly affect the human psyche

– they significantly reduce the efficiency, durability and safe operation of technical works

– anthropogenic (negative) effects seriously harm geological environment

We can conclude that the geopotential generally improve the quality of human life and geobarriers reduce this quality.

AcknowledgementThe contribution was prepared with help of grant no. 1/0533/14 agency VEGA.

Prof. Dr. František Baliak, PhD., Mgr. Martin Brček, PhD.Faculty of Civil Engineering of Slovak University of Technology, Department of Geotechnics,Radlinského 11, 831 05 Bratislava, Slovak republicE-mail: [email protected], [email protected]

References1. Baliak, F., Ondrášik, M., Brček, M.: Rock

environment and its impact on the human. In: Acupuncture and Natural Medicine. 2015, 1, p. 33–39, MSNM, Bratislava, ISSN 1339­4703.

2. Baliak, F., Khun, M.: Geofaktory životného prostredia a ich vplyv na človeka. In: Zborník abstraktov. XVI. Lekársky kongres naturálnej medicíny. Nitra, 2012, s. 6 – 7.

3. Baliak, F., Stríček, I.: 50 rokov od katastrofálneho zosuvu v Handlovej. 50 yars after catasrofiphic landslides in Handlová (Slovakia). Mineralia Slovaca, 2012, 44, s. 119 – 130, ISSN 1338­3523, ISSN 0369­2086.

4. Baliak, F., Ondrášik, M., Brček, M.: Water as important geological factor of the environment of Slovakia. In: Procedine Príspevok je jedným z výstupov grantových úloh č. 1/0533/14 agentúry VEGA, s. 13, International Symposium on Water Management.

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Interdisciplinary InsightsSlope Movements as a Geofactors of the Environment and their Impact on Human

Prof. Dr. František Baliak, PhD., Mgr. Martin Brček, PhD.

5. Nemčok, A., Pašek, J., Rybář, J.: Dělení svahových pohybů. In: Sborník geologických věd, 1974, 11, Praha.

6. Ondrášik, R., Vlčko, J., Fendeková, M.: Geologické hazardy a ich prevencia. UK Bratislava. 2011, s. 228, ISBN 978 80 223­2956 9.

7. Šimeková, J., Martinčeková, T. et al.: Atlas máp stability svahov SR v M 1:50 000. Záverečná správa z orientačného IG prieskumu, INGEO – ighp, s. r. o., Žilina, 2006.

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Interdisciplinary InsightsFluorine and Health Problems in Žiarska kotlina Basin (Slovakia)

Assoc. Prof. Dr. Miloslav Khun, CSc.

Fluorine and Health Problems in Žiarska kotlina Basin (Slovakia)

Assoc. Prof. Dr. Miloslav Khun, CSc.

SummaryFrom the monitored diseases and contaminated areas we presume that a causal relationship might exist between concentration of fluorine in the environment and the high rate of occurrences of tumors, diseases of endocrine glands and congenital malformation in studied population of children aged  0–14  in the time span of 1987 to 1995. These results and the interpretations presented herein are only a first step, the problem requires another more detailed investigation.

Key Wordsfluorine, medical geochemistry, child population, Žiarska kotlina basin

The enterprise for aluminium production ‘ZSNP’ (from the year 1993 Slovalco corporation) in the middle­Slovakian town Žiar nad Hronom with its emissions does not only effect the near vicinity but entire Žiarska kotlina basin. Fluorine is here the most dangerous pollutant. The highest pollution of fluorine in the factory vicinity occurred in the sixties of last century. The  village of Horné Opatovce had to be moved. In  the seventies of last century when absorbers were introduced into technology of aluminium production, the average annual emission of fluorine dropped from 8 μg.m−3 in 1973 to 3 μg.m−3 in 1987, which was still three times greater than the acceptable limit.

In  the affected area deleterious influences were observed mainly on the soil cover, flora, fauna and, what is worse, on the child population. These effects were demonstrated by the more

frequent problems with the respiratory systems, stains on teeth and changes in skeletal tissue and blood. The  increased content of fluorine in all monitored tissues was seen in the entire indigious population.

The situation has been changed by modernization of production operation after year 1990. New technology established in 1995 has reduced the concentration of fluorine in exhaust below the limit 1  μg.m−3 which is not harmful for the environment.

Topography of former district Žiar nad Hronom, geological and geochemical characteristics of Žiarska kotlina basin as well as further informations a reader can find in following papers: Bímová (1995); Čurlík (1995); Hlavnička (1992); Khun et al. (1997); Konečný a Lexa (1984) a Múdry et al. (1992).

The input data are about the population of children aged 0–14 that was collected from pediatric clinics of hospitals Žiar nad Hronom (ZH), Nová Baňa (NB), Banská Štiavnica (BS) and Kremnica (KR) during 1987–1995. At  disposal were data of 13  groups of diseases classified in sense of ICD­10, which emits  WHO: I. to VI., IX. to XIV. and XVII. For this contribution only three groups of diseases with pressumed impact of fluorine from emissions and by it polluted abiotic components of the environment were selected (soils and agricultural crops; not drinking water which meets valid legislative): II.  – neoplasms; IV.  – endocrine, nutritional and metabolic diseases; XVII.  – congenital malformations,

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Assoc. Prof. Dr. Miloslav Khun, CSc.

deformations and chromosomal abnormalities. Note: The tributary area of mentioned hospitals included 20 cadastre territories of municipalities.

In  table  1 are presented numbers of medical cared children aged 0–14 in the selected groups of diseases (ICD­10) and  their percentual rates on bulk morbidity in tributary area of former district Žiar nad Hronom.

Tab. 1 Number of medical cared children aged 0–14 in selected three groups of diseases and their percentual rate on bulk morbidity in tributary area of former district Žiar nad Hronom.

ICD-101987 – only pre-school age

ZH NB BŠ KRAbs. % Abs. % Abs. % Abs. %

II. neoplasms 3 0.55 1 0.36 – – – –IV. endocrine diseases 10 1.83 6 2.18 7 2.76 3 1.59XVII. congen.malform. 31 5.68 22 8.00 30 11.81 14 7.41Total number of diseases in all 13 groups 546 100.00 275 100.00 254 100.00 189 100.00

ICD-101988

ZH NB BŠ KRAbs. % Abs. % Abs. % Abs. %

II. neoplasms 11 0.39 7 0.52 3 0.22 1 0.06IV. endocrine diseases 217 7.72 52 3.85 55 4.10 24 1.43XVII. congen.malform. 99 3.52 37 2.74 65 4.85 53 3.15Total number of diseases in all 13 groups 2,811 100.00 1,350 100.00 1,340 100.00 1,684 100.00

ICD-101989

ZH NB BŠ KRAbs. % Abs. % Abs. % Abs. %

II. neoplasms 9 0.32 5 0.42 3 0.25 – –IV. endocrine diseases 227 7.98 41 3.44 49 4.05 19 1.43XVII. congen.malform. 89 3.13 35 2.94 50 4.14 40 3.01Total number of diseases in all 13 groups 2,844 100.00 1,191 100.00 1,209 100.00 1,327 100.00

ICD-101990

ZH NB BŠ KRAbs. % Abs. % Abs. % Abs. %

II. neoplasms 9 0.31 5 0.50 2 0.16 – –IV. endocrine diseases 209 7.22 29 2.89 56 4.57 13 1.67XVII. congen.malform. 274 9.46 92 9.17 93 7.59 44 5.66Total number of diseases in all 13 groups 2,895 100.00 1,003 100.00 1,226 100.00 777 100.00

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Assoc. Prof. Dr. Miloslav Khun, CSc.

ICD-101991

ZH NB BŠ KRAbs. % Abs. % Abs. % Abs. %

II. neoplasms 18 0.38 – – 3 0.26 – –IV. endocrine diseases 257 5.36 – – 47 4.13 – –XVII. congen.malform. 436 9.10 – – 3 5.53 – –Total number of diseases in all 13 groups 4,792 100.00 – – 1,139 100.00 – –

ICD-101992

ZH NB BŠ KRAbs. % Abs. % Abs. % Abs. %

II. neoplasms 16 0.60 5 0.50 3 0.26 2 0.25IV. endocrine diseases 192 7.14 30 2.98 47 4.13 13 1.64XVII. congen.malform. 277 10.30 9 0.89 63 5.53 58 7.30Total number of diseases in all 13 groups 2,689 100.00 1,007 100.00 1,139 100.00 794 100.00

ICD-101993

ZH NB BŠ KRAbs. % Abs. % Abs. % Abs. %

II. neoplasms 16 0.62 4 0.35 2 0.18 2 0.24IV. endocrine diseases 175 6.78 29 2.51 48 4.42 13 1.59XVII. congen.malform. 281 10.89 112 9.71 16 1.47 60 7.34Total number of diseases in all 13 groups 2,581 100.00 1,154 100.00 1,086 100.0 817 100.00

ICD-101994

ZH NB BŠ KRAbs. % Abs. % Abs. % Abs. %

II. neoplasms 16 1.01 3 0.50 2 0.45 1 1.14IV. endocrine diseases 127 8.04 22 3.64 24 5.45 7 0.96XVII. congen.malform. 259 16.40 92 15.21 23 5.23 37 5.08Total number of diseases in all 13 groups 1,579 100.00 605 100.00 440 100.00 728 100.00

ICD-101995

ZH NB BŠ KRAbs. % Abs. % Abs. % Abs. %

II. neoplasms 11 1.09 3 0.44 2 0.45 1 0.15IV. endocrine diseases 85 8.45 30 4.39 24 5.45 7 1.02XVII. congen.malform. 129 12.82 80 11.71 23 5.23 1 0.15Total number of diseases in all 13 groups 1,006 100.00 683 100.00 440 100.00 689 100.00

Explanations: hospitals: ZH – Žiar nad Hronom; NB – Nová Baňa; BS – Banská Štiavnica; KR – Kremnica. Abs. = absolute number of diseases; % – percentual rate from all 13 group of diseases; – no data.

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Assoc. Prof. Dr. Miloslav Khun, CSc.

1987 1988 1989 1990 1991 1992 1993 1994 1995

1.2

1.0

0.8

0.6

0.4

0.2

0.0

ZHNBBŠKR

Fig. 1 I. Neoplasms (% rate on bulk morbidity)

1987 1988 1989 1990 1991 1992 1993 1994 1995

1.0

2.0

3.0

4.0

5.0

6.0

7.0

8.0

9.0

0.0

ZHNBBŠKR

Fig. 2 IV. Endocrine diseases (% rate on bulk morbidity)

1987 1988 1989 1990 1991 1992 1993 1994 1995

2.0

4.0

6.0

8.0

10.0

12.0

14.0

16.0

18.0

0.0

ZH

NB

KR

Fig. 3 XVII. Congenital malformations (% rate on bulk morbidity)

From three monitored groups of diseases upper­most rates of morbidity in tributary area of the hospital in Žiar nad Hronom can be stated. As  far as high rate of cancer diseases in former Žiar nad Hronom district (Fig.  1), there are

highest morbidity and mortality from thyroid gland cancer also in adult population in Slovakia. Fluorides namely accelerate the cancer process fatal by the input of glucose in cancerogenic cells. This leads to immunossuppresion. Similarly, it can consider by the diseases of endocrine glands (Fig. 2) the changes due displacement of iodine by fluorine on the ground of experiments with other halogen element (Velický et al., 1997). High rate of congenital malformations in tributary area of Žiar nad Hronom hospital can be result of genetic predisposition, but also with appreciable contribution of fluorides. The  concentration of fluorides as pollutants is uppermost in this tributary area.

Children living in Žiarska kotlina basin have often diagnosed anaemia of microcythanaemia. Every fourth child aged 7 is in some phase of anaemia development, probably due to the impact of toxic fluorine exhalations to enzymatic systems.Hair are considered as indicator of long­term fluorine exposition. The  analysis of fluorine in urine serves as more precise biological exposition test. The  treshold value is 1  mg of fluorine in 1  litre of urine. Dr.  Turčanová has given kindly the following data from her research:

Tab. 2 Fluorine contents in urine of girls and boys aged 10–14 from monitored cadastre territories (E. Turčanová, November 1997 – personal communication)

Municipalities cadastre territories

Girls aged 10–14

Boys aged 10–14

F mg.l−1 urine F mg.l−1 urine

Žiar nad Hronom

0.25 0.400.280.41

Hliník nad Hronom

0.47 0.890.31

Lovča 0.43

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Assoc. Prof. Dr. Miloslav Khun, CSc.

Municipalities cadastre territories

Girls aged 10–14

Boys aged 10–14

F mg.l−1 urine F mg.l−1 urineLehôtka pod Brehmi 0.48 0.30

Ladomerská Vieska 0.61 0.63

Though a treshold wasn’t crossed in any case, it  is interesting uppermost content of fluorine 0.89 mg.l−1 from municipality Hliník nad Hronom. Just here high fluorine contents in soils which overcrossed treshold legislative values were analysed. Well, a  devote attention is necessary apart mentioned indications on potentially impact of fluorine to morbidity from some group of diseases also to his input from food. We mean the agricultural crops growed on polluted soils of Žiarska kotlina basin.

ConclusionWe  can state with respect to concentration of fluorine in the Žiarska kotlina basin environment (20  cadastre territories) and  to relationship of monitored selected disease groups in child population aged 10–14 following:

– uppermost morbidity of monitored child population in all selected disease groups in tributary area of Žiar nad Hronom hospital in comparison to other tributary areas. Just here the abiotic components of the environment as well as agricultural crops are uppermost polluted with fluorine,

– high share of tumors (see, for  instance, high morbidity and mortality due to tumors of thyroid in adult population of former district Žiar nad Hronom in time period 1968–1984, or high rate of mortality due to not specified tumor diseases in time period 1983–1992 in comparison with overall Slovak average),

– diseases of endocrine glands, mainly changes of thyroid where displacement of iodine by fluorine as main agent can be assumed,

– high rate of congenital malformations (genetic predispositon and probably appreciable contribution of fluorides),

– we consider these results and the interpretations for the first approach only and this problem requires another detailed research.

AcknowledgementThis contribution is the result of the project implementation: Comenius University in Bratislava Science Park supported by the Research and Development Operational Programme funded by the ERDF Grant number: ITMS 26240220086.

Assoc. Prof. Dr. Miloslav Khun, CSc.Dpt. Of Geochemistry, Faculty of Natural Sciences, Comenius University in Bratislava, Mlynska dolina, 842 15 Bratislava, Slovak republicE-mail: [email protected]

References1. Bímová, D.: Charakteristika okresu Žiar nad

Hronom. In: Geofaktory životného prostredia regiónu Žiarska kotlina a Banskoštiavnická oblasť. MŽP a GÚDŠ Bratislava, 1995, s. 9 – 14.

2. Čurlík, J.: Niektoré aktuálne problémy ochrany pôd regiónu Žiar nad Hronom. Geofaktory životného prostredia regiónu Žiarska kotlina a Banskoštiavnická oblasť. MŽP a GÚDŠ Bratislava, 1995, s. 25 – 27.

3. Hlavnička, V.: Zdravotnícka situácia obyvateľstva v spádovej oblasti Žiaru nad Hronom. In: Životné prostredie v Žiarskej kotline, 1992, nestr.

4. Khun, M., Jurkovič, Ľ., Urminská, J.: Charakteristika zdravotného stavu detskej populácie v regióne Žiarskej kotliny z aspektu medicínskej geochémie. Záverečná správa za II. etapu projektu Zhodnotenie ekologickej únosnosti regiónu Žiarskej kotliny. Katedra geochémie PriF UK Bratislava, 1997, s. 37.

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Assoc. Prof. Dr. Miloslav Khun, CSc.

5. Khun, M.: Kontaminácia zložiek životného prostredia regiónu Žiarskej kotliny fluórom. Manuskript – nepublikovaný interný materiál, Katedra geochémie PriF UK Bratislava, 2002, s. 13.

6. Konečný, V., Lexa, J.: Geological map of central Slovakia Neogene volcanic field. GÚDŠ Bratislava, 1984.

7. Múdry, P. a kol.: Ekologická únosnosť regiónu Žiarskej kotliny, II., Ekotrust, Banská Štiavnica, 1992, s. 369

8. Velický, J., Titlbach, M., Dušková, J., Raška, I.: Účinek bromidu draselného na štítnou žlázu potkana. In: Zborník XIV. Biologické dny, Praha, 1997, Univerzita Karlova, PS 12/1.

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Dr. Denisa Šoltésová, PhD., Mgr. Michaela Skyba, PhD.

Human, Animal and Therapy

Dr. Denisa Šoltésová, PhD., Mgr. Michaela Skyba, PhD.

SummaryThis paper briefly presents selected characteristics in the research field of human­animal interactions and specifically animal assisted therapy. In  the introduction it summarizes the historical contexts of scientific research in this area. The main attention is devoted to the benefits of human­animal interactions in their therapeutic application. The paper is based on the conceptual framework of bio­psycho­social model of health. Based on the available professional literature it summarizes especially physiological effects of animal assisted therapy. As  selected model postulates the benefits are derived from them in mutual determinations in the context of psycho­social domains of health and quality of life. Contributions are defined at the end of this part of paper. Besides the traditional, generally more acceptable, evidence­based scientific approach, for future research and the application the theory of morphic resonance appears to be inspiring. It  draws from holistic approach and specifics of different morphic fields and creates a new perspective in appreciation of human­animal interaction and their therapeutic use. At the end the paper offers brief description of this theory in the context of the topic.

Key Wordsbiopsychosocial model for health, morphic resonance theory, human­animal interactions, animal assisted therapy, canine assisted therapy (caninetherapy).

Human-Animal Interactions as the Subject of Scientific InterestA  human with his biological background is part of the natural community and so the natural environment (including the geophysical, chemical, animalistic  etc.) becomes his obvious context. Relations between humans and animals have existential, biological, ethological, psychosocial evolutionary history1. Despite the common coevolution of humans and animals legitimate research attention is interested in the field of human­animal interactions (HAI) just a several decades (primarily especially anthrozoology but also psychology, human and veterinary medicine, sociology, etc.).

Professional literature most often links the beginnings of research activities in this field with publications of Levinson in the 60s of the 20th century (especially contribution The Dog and as Co­Therapist, published in  1962 in the journal Mental Hygiene, and a monograph Pet­Oriented

1 About the ethological aspects of the human­animal

interactions in the Slovak conditions more Hvozdík (2002),

Hvozdík, Bučková (1999) and others.

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Child Psychotherapy 1969)2. The several experts (eg. Wilson, Turner, 1998; Fine, 2000, 2010; Chandler, 2012; Odendaal, 2007; McCardle et  al., 2011 et  al.) consider that Levinson conceived the starting points of the new scientific discipline Human­Companion Animal Bond (currently HAI). Despite the initial skepticism of experts mainly from the environment of public health, research in the field of HAI and especially their therapeutic application in the global context rapidly developed in later period (especially from the 80s of the 20th century).

In 1984, based on a meta­analysis of publications in this area Beck and Katcher (in: Beck, Katcher, 1996) stated that primarily there are descriptive or on hypotheses based studies. According to the authors experimental findings arguing HAI positive benefits for human health absented. On  the other hand they did not exclude the possibility of facilitation of other conventional therapies through the incorporation of interactions with animals. According to the authors, HAI  therapeutic effects were rather emotional response of patients to the presence of animals, what justified their usage rather for the recreational purposes, than it had been clearly demonstrated relevant health benefits.

2 In the past basis were already conceived by Buck (1903, in:

Serpell, 1996) and Bossard (1944, in: Rowan, Thayer, 2000).

In the academic contribution Buck analyzed 1,200 essays

of children about their dogs and he found out that children

perceive dogs as a significant source of unconditional

acceptance and affection in situations of loneliness

and helplessness (Serpell, 1996). Bossard discussed the

importance of pets for family life and mental health of the

family members, especially in relation to the development of

children and defined the various roles, that dog can play in

the family (e. g. “serve” as a social lubricant, dog is a source

of unconditional love, partner, “the teacher” of children in

practicing good hygiene practices, helps to enhance self­

esteem, develops empathy, etc. (Rowan, Thayer, 2000).

Since the 80’s the (multidisciplinary) research has been intensified with focus on two levels  – in  the context of health promotion (and overall quality of life) of pet owners and in connection with the specifics of the newly­constituted type of animal assisted therapy (Serpell, 1996, 2000; Fine, 2000, 2010; Odendaal, 2007; Chandler, 2012). Mainly findings of Friedman et al. (1980, in: Friedmann, 2000; Friedmann, Thomas, 1998) were considered as an incentive in the context of significant correlations HAI with selected factors of the health status of persons with cardiovascular diseases.

In  this period many organizations focusing on this issue were established. Their activities were gradually profiled in scientific research activities and in connection with their practical implementation. Currently the most important in the United States and Canada (with a European overlap) are  Delta Society (from  2012 under the name Pet Partners), International Society For Anthrozoology (ISAZ), American Humane Association, International Society for Animal-Assisted Therapy (ISAAT), International Association of Human-Animal Interaction Organisations (IAHAIO)3 and others. Today the most important organizations in Europe are Institute for Interdisciplinary Research on the Human-Pet Relationship (IEMT) (Austria, Switzerland), Tiere helfen Menschen, Therapiehunde Deutschland (Germany) and European Society for Animal Assisted Therapy (ESAAT)4 (Austria).

3 It was established in 1992. At present it is the center of

academic and research activities, the leader and the umbrella

organization of HAI.4 ESAA is an umbrella organization for the field of animal

assisted therapy and activities in Europe. It was established

in 2004 in Vienna where resides to this day.

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These findings also supported research interest aimed at the mechanisms of influence of HAI on human health and formulation of key theories about their effects on quality of life. Mainly two hypotheses were postulated  – the  ability of animals to affect, induce of current level of distress through detachment and keep attention from own illness or disability (Katcher et  al., 1983, in: Serpell, 2000) and the ability to provide social support reducing stress experience (stress-reducing/stress-buffering) (McNicholas, Collis, 1998; Serpell, 1996; Siegel, 1990, in: Beck, 2000 etc.).

In  1987  the National Institutes of Health (at  the conference The Health Benefits of Pets) has defined five areas for intensive research of HAI, and benefits of HAI  for people with cardiovascular disease, health benefits of HAI for the elderly, social and therapeutic benefits of  HAI, risks arising from HAI and the role of animals in relation to (social, emotional, cognitive) development of human personality (and in various stages of development in the context of the whole life cycle). On  the basis of meta­analyzes of completed research several experts (eg. Garrity, Stallones, 1998; Hines, Frederickson, 1998; Wilson, Turner, 1998; Fine, 2000, 2010; Beck, 2000; Johnson, Odendaal, Meadows, 2002; Morrinson, 2007; Knight, Edwards, 2008; Herzog, 2011; McCardle et  al., 2011; Chandler, 2012; Chur­Hansen, Zambrano, Crawford, 2013 etc.) document that except the last of those topics we have currently sufficient professional and scientific argumentation in favor of positive impacts of interactions with animals (especially companion) on  health and quality

of life of people.5 Current empirical evidence is still limiting our detailed understanding of the reciprocal effect of HAI, especially correlated with the health and quality of life. However, current knowledge base already creates a solid evidence and argument basis of positive benefits.

In  this context we will devote attention not on the entire spectrum of HAI  in their width and variable, but  the selected partial area  – therapeutic application. Although in the Slovak and Czech Republics it is common to use the term Zootherapy (or animotherapy), this is confusing6. Therefore we based on the definitions proposed by the working group IAHAIO (IAHAIO White Paper: The  IAHAIO Definitions for Animal Assisted Intervention and Animal Assisted Activity and Guidelines for the Wellness of Animals Involved 2013), that differ intervention from activities with the participation/with assistance/with inclusion of animals (Animal Assisted Intervention/Animal Assisted Activities7).

5 On the other hand, e.g. Garrity and Stallone (1998) have

expressed doubts about the existence of relevant evidence

about direct causal effect of HAI on human health. According

to the authors positive results rather correlate with a factor of

social support of people in contact with animals. The authors

supported mainly mediated influence (buffering mechanism),

but also have not denied the possibility of a direct causal effect.6 In practice and in research it causes misunderstanding,

misinterpretation and false expectations in the level of

qualification and personal characteristics of the persons

providing the services, the assumption of animals included

into services and the expected effects – often seen as a

uniquely targeted therapeutic interventions, which is not

always consistent with the reality.7 In the past also concepts pet therapy, pet­facilitated therapy

(animal­facilitated therapy), pet­mediated therapy, human/

companion animal therapy, co­therapy with an animal, four­

footed therapy, or in specific areas pet­oriented psychotherapy,

animal­assisted psychotherapy, animal­assisted counseling,

animal­assisted social work, animal­assisted nursing, animal­

facilitated counseling, animal­assisted support services etc.

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Animal Assisted Interventions are goal­oriented interventions that intentionally incorporate “animals to the health, education and human services8 (eg. social work) in  order to achieve therapeutic effects on humans’ lives. Animal assisted interventions incorporate human-animal teams into formal human services” in two ways, as an animal assisted therapy or animal assisted education (The IAHAIO Definitions… 2013, p. 4).

Animal Assisted Therapy (Animal Assisted Therapy  – AAT) is  “goal-oriented, planned and structured therapeutic intervention which is implemented/managed by professionals from the fields of medicine, education or human services. The  progress of intervention is measured and recorded in the technical documentation. AAT are realized by helping professionals with formal qualifications and expertise within their practice. AAT  is aimed at strengthening the physical, cognitive, behavioral and/or socio-emotional functioning of a particular client” (The  IAHAIO Definitions…, 2013, p. 4). It was originally defined in Standards of Practice for Animal-Assisted Activities and Animal-Assisted Therapy (1996, p.  79) as  intervention, “in  which the animals meeting the specific criteria are an integral part of the healing process. Animal Assisted Therapies are provided and/or managed by providers of health or social service within their profession. This therapy is planned as a mean of supporting the enhancement of human physical, social, emotional

8 From English language human services – programs and

activities aimed at supporting of people and their well­being,

especially social services, health services, education, area of

housing, income, justice and public safety.

and/or cognitive functions. Specific objectives must be fixed by professionals and process and progress documented, measured, recorded and evaluated”9.

It  is an intentional incorporation of animals into the therapeutic plan and process due to facilitation of therapy and recovery of people with acute or chronic diseases. The  variability of traditional therapies is increased by their application. The  anticipate benefits are for example health benefits, the  development of cognitive, physical and social skills, emotional regulation, and so on.10

Bio-Psycho-Social Model as a Conceptual Framework of HAIIn  the current professional literature several theories and hypotheses explaining the mechanisms of positive benefits of HAI  on

9 The key characteristics of AAT are: a) implementation is

supervised by a specialist within own profession, and the

animal can be kept also by volunteers who act according the

instructions of expert, b) may be included in the therapeutic

process only in the context of specialization of helping

professional, c) focus on specific therapeutic objectives,

d) are properly monitored and documented (Student

Manual…, 2008).10 In practice there are more often realized recreationally,

motivationally or educationally (not therapeutically) targeted

activities with the assistance of animals (Animal Assisted

Activities – AAA) – activities “providing opportunities for

motivational, educational and/or recreational effects that

enhance the quality of life”, and “the same activities can be

repeated for different persons, in contrast to the therapy which

is tailored to a particular person or medical event” (Standards

of Practice …, 1996, p. 79). They are usually realized in the

form of informal interactions, often carried out on a voluntary

basis (through the persons who are not educated in the health,

education and human services) (The IAHAIO Definitions…,

2013, p. 4–5) and usually not provide scientific results. They

do not required specific treatment objectives or detailed

documentation, the content is more spontaneous and may

take only a few minutes (Student Manual…, 2008).

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health and quality of life of people are postulated (especially the attachment theory, concept of social support, the  concept of evolution of behavioral interactions, respectively theory “attentionis egens”, theory of biophilia and more recently formulated “animate­monitoring hypothesis” a “life­detector hypothesis”)11. Some of them were originally designed for human­human interaction, and  subsequently applied in new contexts of  HAI. We  present closer bio­psycho­social model, which seems to be a comprehensive conceptual framework for the purposes of this paper.

The  structure for understanding of HAI  may provide bio­psycho­social­cultural­spiritual model particularly in the context of the (positive) impact on human health with emphasize on the interactive nature of the biological, social, psychological and spiritual areas, which are interrelated and together they create health status and other conditions for optimal social functioning. In  the context of this model, we can see that the animals as part of the human environment have the potential to affect function of all areas, physiological status, psychosocial and spiritual characteristics in a particular cultural context (Friedmann, Barker, Allen, 2011).

Bio­psycho­social model by George  L. Engel (1977, 1980) was created in response to the need for a new medical approach in the 70’s of the last century. Author reflected and highlighted in the perception of health and disease mutual interactions of biological, psychological and social factors. In  addition to the biological aspects (of disease) he accents also psychosocial and cultural dimensions. His initial concept was gradually elaborated and included cultural and spiritual aspects of health, and the broader context

11 Several of them are briefly characterized and in comparison

with the theory of “attentionis egens” discussed by Odendaal

(2007).

of family, community, and  culture and society structure12 (Hatala, 2012; Hutchinson, 2015 a, b). The  model integrates multi­level, biological, psychological, social, cultural and spiritual domains (in their mutual determinations) within a broader historical socio­political and cultural context and represents the optimal framework for a deeper understanding of the issues of client/patient and allows to explore five domains of life in its specific environment (Hutchinson, 2015 a, b).

In this model, animals are conceptualized in the psychosocial sphere as a form of social support, which may affect other spheres, and finally also health. Animals can directly affect physiological processes, reduce depression and anxiety, enhance social support and affect physiological processes. Interaction with calm and friendly companion animal is mean of reduction of physiology of chronic stress caused by loneliness, anxiety, depression, and  physiological stress responses caused by acute stressors.Psychosocial distress, including loneliness, anxiety, depression (also due to a lack of social support) is  associated with hyperactivity of the hypothalamic­pituitary­adrenocortical system  / axis and sympathetic­adrenomedullary system / axis. Activation of HHA system causes secre­tion of corticosteroids in the blood, while hyperactivity of SAS causes an increase level of catecholamine, reduction of the variability of heart rate, decrease of myocardial perfusion and ventricular instability, disruption of functions of immune system. This paradigm provides a

12 Multifactoriality of model rejects reductionism in which

the spiritual and cultural dimensions should be “only” part

of the social and psychological dimension, arguing their

particular value and current trends in research in the field

of intercultural, multicultural, transcultural psychology

and etnopsychiatry (Hatala, 2012). Despite this, due to the

contribution limits we will not discuss the cultural and

spiritual contexts of HAI.

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framework for understanding how psychosocial, behavioral and physiological (neuroendocrine) interactions affect the immune system. In  the activation of the HHA and SAS systems, chronic stress induces suppression of the immune system, increases vulnerability towards infections what increases the probability of chronic morbidity and mortality. Epidemiological and experimental researches contribute to the understanding of the physiological effects of presence and interaction with animals. In this research interest is focused on the cardiovascular system and the components of the stress responses (blood pressure, cortisol, epinephrine, norepinephrine, heart rate, immunoglobulin  A, lymphocyte proliferation) (Friedmann, Barker, Allen, 2011).

On  the basis of repeated experimental studies Odendaal (2007) and  Uvnäs­Moberg, Handlin, Petersson (2011) confirm the importance of canine assisted therapy in the field of physiological, endocrine and behavioral variables. Particularly they focus on oxytocin as hormone produced in response to bonding, relation behavior (along with the effect of inhibition of the stress and stimulation of social interaction). The  oxytocin fibers reach the different areas of the central nervous system that control the various functions, and lead to a number of behavioral and physiological effects – reduce anxiety through the influence of the amygdala, reduce the perception of pain, neutralize aggression and agitation, inhibit the secretion of cortisol (produced in response to stress, increasing the blood pressure, glucose level with the immunomodulating effects). It  also increases the activity of some important aspects of the sympathetic nervous system regulating the cardiovascular system, which leads to the decrease of blood pressure and increase the peripheral skin circulation and skin temperature. It  also enhances the function in some aspects of the parasympathetic nervous system checking the functions of the endocrine

system of the gastrointestinal tract, which results in strengthening of digestive functions. It  influences the social interactive behavior (and is produced in response to the confidential contact and possibly also in response to social support), stimulates the bonding (also) in some non­human species. Vasopressin, which is related to oxytocin also plays a role in bonding. It  has important modulator effects on social behavior and coping with stress. Odendaal (2007) also points to significant changes in the levels of the various neurochemical elements and enhancement of endorphins, oxytocin, prolactin, phenylacetyl, dopamine.

As  stated by Friedmann, Barker, Allen (2011) several current researches confirm that the presence of the animal directly affects the stress experience (animal as absorber or moderator of stress responses to acute daily stress), what is presenting in the immediate modification of physiological stress indicators such as reduction of systolic arterial pressure and capillary pressure and reduction in epinephrine and norepinephrine levels during and after the intervention with dog.

At  the level of physiological effects of HAI  to human health further studies (Friedmann et al., 1980, in: Friedmann, Barker, Allen, 2011; Bolin, 1987, 1988, in: Wood et  al., 2005; Grossberg et  al., 1988, in: Beck, 2000; Collis, McNicholas, 1998; McNicholas, Collis, 1998; Allen et  al., 1991, in: Beck, 2000; Anderson et  al., 1992, in: Rowan, Thayer, 2000; Walsh et  al., 1995, in: Chandler, 2012; Crowley­Robinson et  al., 1996, in: Rowan, Thayer, 2000; Friedmann, Thomas, 1998; Friedmann, 2000; Wagner et  al. 2007, in: Fine, 2010; Odendaal, 2007 etc.) document faster recovery and prolong survival time of patients with cardiovascular disease; faster wound healing; reduction of stress before and after surgery; reduction of pain; reduction in the level of blood pressure; inhibition the creation

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and development of psychosomatic diseases; decreasing of cholesterol level and plasma triglyceride; reducing the level of stress and anxiety at  all; effective adaptation to stressful situations; alleviating depressive states; changes in neurohormonal production; reducing the consumption of drugs (especially antidepressants and anxiolytics); reducing the frequency of doctor visits; reducing the number of days spent in hospitals; reducing the number of days of sick leave and other positive effects in this area.

Also, benefits are derived from the physiological effects in the context of the psycho­social sphere of health and quality of life. In  this aspect the research is focused on determinants of mental health and specific psychological states and processes as well.

Social stress situations, anxiety, psychosocial stress are accompanied by adverse physiological reactions and increased susceptibility to illness. In  these situations animals can be a source of support and unconditional acceptance (Triebenbacher, 1998, in: Wilson, Turner, 1998; Fitzgerald, 2007; Morrison, 2007; Herzog, 2011; Chandler, 2012), or  may be a substitution for human support (Collis, McNicholas, 1998; McNicholas, Collis, 1998; Zasloff, Kidd, 1994, in: Johnson, Odendaal, Meadows, 2002). Within the psychosocial benefits of animal assisted therapy there are proven also other, especially positive effect on individual cognitive functions; development of social skills and interest in social environment; support of psychosocial functioning; support of pro­social behavior and personal responsibility; reduction of loneliness; reduction of depressive states; development and strengthening of social networks or compensation of their deficiencies; promotion of effective communication; positive effect on self­esteem, self­respect and self­control; reduction of rough behavior or tendency to such behavior; saturation of some basic psychological

needs (to be loved, respected, needed, accepted, etc.); facilitation of social interactions and social inclusion; stimulation of reciprocal behavior in groups; creation of opportunities for spending leisure time usefully; relation with animals has the potential to give the value to life and future perspectives; animals in facilities facilitate social and verbal interactions and positively affect the overall atmosphere in a particular facility  etc. (Fine, 2000, 2010; Chandler, 2012; Sobo et  al., 2006; Knight, Edwards, 2008; Greisler, 2004; Triebenbacher, 2000; Herzog, 2011; Morrison, 2007; Chur­Hansen, Zambrano, Crawford, 2013; Wilson, Turner 1998; Wood et  al., 2005; Odendaal, 2007; Fitzgerald, 2007; Parish­Plass, 2008 etc.)13.

The Theory of Morphic ResonanceAlthough the Rupert Sheldrake’s theory of morphic resonance is currently not clearly and comprehensively accepted by the scientific community, it  seems to be another possible explanation of the positive benefits of human­animal interactions (in  relation to people and animals as well), with scientific and application dimension. At the same time, we believe that this theory is explicitly compatible with the principles of natural medicine.

The  theory of morphic resonance understands natural systems (morphic units) at  all levels of complexity as an organized and coordinated by morphic fields, that contain inherent memory – all  the self­organizing systems (from crystals to human society) inherit collective memory, which affects their form and behavior. Morphic resonance is the process of transferring information from the previous to the next system and influences of like upon like are not limited by space. According to the hypothesis of

13 In the literature we can find also the perception of companion

animals as social capital (Wood et al., 2005; Šoltésová,

2014 a, b).

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formative causality in the self­organizing systems of varying degrees of complexity there is integrity dependent on characteristic organizational field of the system (morphic field). Each of such system is made up of parts that are themselves units of lower system and morphic field gives each unit at each step its characteristic features14 (Sheldrake, 1987a, 2003, 2004).

Morphic resonance is the basis of inherent memory of fields at all levels of complexity. All  self­organizing systems have a collective memory to which each individual contributes. This is a cumulative memory, based on what has happened to the species in the past. Natural systems inherit this collective memory from all the ancestors of their kind in the process of morphic resonance (past forms and behavior of organisms determine similar organisms at present and patterns in development and behavior become habitual through the repetition15). Morphic resonance is the impact of previous structures of activities on subsequent similar structures of activities that are organized through the morphic fields. It allows the passage of memories through the space and time from the past (Sheldrake, 1987a, 2003, 2004).

Morphic resonance operates through morphic fields that organize organisms (animals, plants…), coordinate brain activity and form the basis of mental activity16. It  is also an explanation of the telepathic type of connections

14 It is possible to distinguish morphogenetic fields, perceptual

fields, molecular and crystalline fields, behavioral and mental

fields, cultural and social fields (Sheldrake 2003).15 The understanding of natural laws and natural habits is also

obvious from this thesis (Sheldrake, 2003).16 More also hypothesis of “extended mind” as the basis of

human perception that provide a new way of thinking

about the human­human and human­animal interactions,

relationships and the world around (Sheldrake 2004).

between organisms17. In the process of morphic resonance there are generated links between similar fields. Growing, developing organisms are created/shaped by fields that are around and in them. Each species has its own fields and in any organism there are fields in the fields. These fields have the inherent memory derived from the previous forms of the same species. Morphic fields determine the organization in inherent indeterminism of systems that are under their influence. They connect different parts of the system, which they organize. They are developing themselves through the repeated structures that they organize, they are still more probable and usually. They compel the systems which they affect to create the typical forms and patterns of behavior. Based on morphic fields there are structures and patterns of activities happening and organizing (reflecting not only the composition of organisms, but also their shape). The fields are influenced by morphic resonance, keep a history of previous structures and patterns of activity and thus shape the future development of fields18. Morphic fields link together parts of

17 Hollý and Hornáček (1998) describe in the context of

hippotherapy different type of transmission between

animals and humans. They distinguish specific (determined

by influence of the horse and his step) and nonspecific

(facilitation) factors of effects of the horse and ride on horse.

To the specifics (in addition to rhythmical transferring of

three­dimensional movement incentives determined by

horse step, move forward as a base of motoric development,

bipedal gait as a base of movement pattern) belong also

unprogrammed bioenergoinformation transmission from

the field of animal to person. It is vigorous strengthening of

weakened man from the field of big healthy animal, at the

level of macro (aura and system of energy centers) and micro­

system (set of acupuncture ways and points). This transfer is

reversible.18 The development of form is result of internal organization of

organism and mutual interactions of morfic fields to which

the organism is tuned. This development may be affected by

genetic mutation (Sheldrake, 1987a).

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systems that are seemingly isolated (fields of social groups interconnect the group members) and make up channels of communication through which organisms can stay in touch at a distance. The  important is perception of the human­human, animal­human relations as an elastic and flexible (Sheldrake 1987a, 2003, 2004). Society has many individuals, so it can operate and respond as a single entity through the characteristics of its morphic fields (in  the context of the social and cultural morphic field it explains also the behavior of crowds, and  football hooligans, lynching mobs etc., but also phenomena such as fads, fashions19) (Sheldrake, 1987b).

According to Sheldrake (2003) the  animal domestication played an important role in the evolution of people. The  first domesticated animal was dog. “Domestication” of dog probably played an important role in the processes of domestication of other species. Although previously this process was particularly beneficial for people, interconnectedness of humans and animals persisted until today  – especially in connection with companion animals that play an important role in the lives of many people, who  are often perceived as family members  – human­animal relations are maintained due to interdependence of human nature with animal nature, not  due to the “necessity” of animals. Companion animals are mutually (just like people) interconnected with other members of own group, while bonds between animals and humans can be seen as a hybrid between animal­animal and human­human relations. Bonds interconnect members of different groups, affect their relations (there are different types of interconnections within species and across

19 Social consciousness or memory is presented in all societies,

for example through rituals that are typical for any society,

in the cultural and religious contexts but also in normal,

everyday situations (e. g. a greeting). These can be explained by

morphic resonance with ancestors (Sheldrake, 1987b).

species). Bonds between animals arise within the social field and social fields link human beings at a distance, underlie of development and dispose with memory20.

Social bonds between humans and domesticated animals are special kind of relations that exist between animals of the same species. There are relations within the morphic fields of social groups. The close bonds are emotionally resonant what we see as a key factor in the effectiveness of animal assisted therapy and particularly canine assisted therapy. Effects or benefits of animal assisted therapy can be explained in this context and with respect to such qualities of animals as their empathy towards people, presented favor, affection, companionship, capability of emotional tuning, providing emotional safety. These symptoms (along with their high sensitivity for the needs, current experiencing or health state of people) are probably for the animals the cause and the consequence of the relations which they create with the people, what is presented for example in the rewarding and stimulating effect on humans21 (Sheldrake, 2003).

Due to the common co­evolution the sensibility of animals and humans are not isolated phenomena. According to the hypothesis of formative causality the effect of morphic fields exceeds the brain activity and interferes into the

20 The social field is one of the classes of morfic field. Human

groups inherit collective memory through morphic fields.

Groups of animals are also kept together and formed by

morphic fields and with own collective memory. Morfic fields

allow the transmission of information from one member of

the group to the other, from animal to animal, from person to

person or from human to animal (Sheldrake, 1988, 2003).21 Telepathic communication occurs for those who have a bond

that is linked to emotions, needs and intentions. Mentioned

may explain the ability of animals to predict seizure epilepsy,

diabetic hypoglycaemic attacks, to diagnose cancer and other

diseases (Sheldrake, 2003).

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external environment, connects us with objects of our perception and can influence them through our internal purpose and attention. The positive effects of animal assisted therapy can thus be seen also with regard to the characteristics of perceptual field (as part of the morphic field) – observer is connected with the object of its perception through this field that is linked with brain activity. Humans and animals are interconnected through the perceptual fields  – what explains their interaction22 (Sheldrake, 2003), where healthier animals positively influence a person with a certain type of disability, illness, etc. Since the inherent memory contains the form and patterns of behavior, respectively activities, animal behavior can quickly develop – through the morphic resonance a collective memory is created, and  noticeable adaptation phenomena are particularly evident in domestic animals, what may be related with the growing interest about the therapeutic aspects of human­animal interactions and a growing number of such oriented therapy teams.

ConclusionReflecting the physiological and psycho­social benefits of HAI  in their mutual determination, as we have described above, the animal assisted

22 Wilson (1984) also describes this effect based on the

description of the background of biophil theory.

therapy can be incorporated effectively into the therapeutic process23 (in  the recent decades phenomenon “Caninetherapy” is strongly manifested in Slovak practice). One  of the key factors of its low acceptance as a serious complementary therapeutic approach is lack of relevant empirical findings in Slovakia about its actual benefits, opportunities and risks. It  is reasonable to assume that the relevant scientific research would facilitate its effective and erudite use in the practice of helping professions24.

One  of the background that can provide a framework for understanding of HAI  in level of the positive impact on human health is the bio­psycho­social model, which emphasizes the interactive nature of the various systems in the life of a particular patient and explains the current state of social functioning, where any development or change in one of them is automatically transferred and affects the final state of the other. The incorporation of intentional, therapeutically­oriented interventions with the assistance of the

23 It is mainly about those: reduction of initial reticence,

uncertainty and anxiety from therapy; facilitation of the

therapeutic relation; motivation to communication and

interaction with the therapist; stimulation of interpersonal

relations; increase motivation for active involvement in

therapy; promotion of the capacity of self­control (also at

the terminal stages) and responsibilities; encouragement

of expression of emotions; activation, stimulation to the

movement and motivation to do physical activity and

rehabilitation; active relaxation; saturation of tactile needs;

meeting the needs of unconditional admission and acceptance;

compensation of lack of social support and provision of

consolation; alleviation of pain; alleviation of feelings of

loneliness; saturation of attention need and more (Baun and

McCabe 2000; Beck, 2000; Fine 2000, 2010; Odendaal, 2007;

Morrison, 2007; Atwood­Harvey, 2007; Parish­Plass, 2008;

Berget, Ihlebaek, 2011; LoBue, DeLoache, 2008, 2010, in:

McCardle et al., 2011; Chandler 2012 etc.).24 About selected characteristics of the current situation of this

issue more Šoltésová (2014b).

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animals has the potential for positive affecting of all systems and the health state of individual in its various aspects.

Such a holistic approach to human­animal inter­

action in the context of its therapeutic utility offers a theory of morphic resonance. Relying on the characteristics of morphic fields, the  process of morphic resonance and hypotheses of formative causality, the  positive effects of reciprocal human­animal interactions can be discussed also in relation to the specifics of co­evolution of humans and animals (especially companion and here mainly dogs) and cumulative memory of social and perceptual fields as part of a wider morphic field.

Argumentation and relevant knowledge base is essential for detailed understanding of the nature of the interactions between humans and animals. Currently sphere of HAI offers a variety of solid empirical findings. It  is constantly in the process of dynamic development. Therefore, further intensive scientific research is necessary, especially in the context of the effects of HAI as separate and also complementary therapies as well as research biomarkers, bio­behavioral and genetic data (here is crucial and important research of biological / physiological and neuro­endocrine responses to human­animal in­teractions for better understanding the impact of these interventions on health).

It  is necessary to see as perspective also further research of energy­informative processes bet­ween humans and animals also in term of natural medicine due to the wide application not only directly in therapeutic activities but also in preventive and diagnostic activities as indicated many empirical studies.

The contribution was published as a part of the grant project VEGA MŠVVaŠ SR Nr. 1/0927/14 Aspects of Professionalization of Canine Assisted Therapy in the Context of the Social Work Theory.

Dr. Denisa Šoltésová, PhD., Mgr. Michaela Skyba, PhD.Institute of Educology and Social Work, Faculty of Arts, University of Presov in Presov, Ul. 17. novembra 1, 080 01 Presov, Slovak republicE-mail: [email protected], [email protected]

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Reports from Professional EventsInsights from the XIX Congress of Natural Medicine, Nitra, 16th–18th of October 2015

Teodor Rosinský, M. D., CSc.

Insights from the XIX Congress of Natural Medicine, Nitra, 16th–18th of October 2015

Teodor Rosinský, M. D., CSc.

As also previous congresses organized by Medical Society of Natural Medicine and Acupuncture Section of Association of Private Physicians of Slovak Republic, this congress was oriented interdisciplinary as well. This surely increased its attractivity and a possibility to learn about the latest knowledge in fields that are seemingly not related to medicine. In  a reverse, professionals from other fields had a possibility to learn the knowledge and problems of medicine.

After obligatory opening speeches of G.  Solar, M. D., President of the Congress and L. Pasztor, M.D., Honorary President of Private Physicians of Slovak Republic, Assoc.  Prof.  Ing. B.  Hučko, PhD., Dean of Faculty of Mechanical Engineering of Slovak University of Technology, Professor R. F. Galiachmetov, PhD. from the Russian Centre of Eye and Plastic Surgery in Ufa and Dr.  M.  Schmieke, a  leading physicist and philosopher from Germany, the Congress started with the first block of lectures.

Dr. Solár started with a survey. He chose a poetic topic “Lights and Shadows of Contemporary Natural Medicine”. He  pointed to the growing cooperation with other medical and non­ medical fields whose results, on  one hand, support concepts of natural medicine, on the other hand they help to understand the mechanisms of action of natural­medical procedures and means of therapy, but also risks of unsuitable natural and anthropogenic phenomena to human health with which conventional medicine does not count as still not taken seriously the role of the psyche in

the tampering and restoring health. One  of the shadows is the persistent misunderstanding of the essence of medical methodologies, which are not generally known and scientist­oriented colleagues fail to understand that the real is not only what is measurable and possible to display but also many other phenomena, known from history and as newly discovered connection. Integrating them into medicine would surely increase its effectiveness.

Known physicist and philosopher Dr. Schmieke from Germany, is the author of Time­Waver. As a diagnostic tool it analyses electromagnetic output of the human body and determines the amount of state parameters of individual organs and functions and as a therapeutic tool by its programs it adjusts these features if they are disturbed. In  the beginning of his speech he presented interesting quantum physics justification of interconnections between the physical and mental plane as well as the energy­information processes. On this principle he created also Time­Waver, whose range of applications he presented. The  first contact evoked a broad debate, where possible integration into the natural medicine and its terminology was analysed as well as the risk of potential abuse of this method.

Dr.  Tarbajovská subsequently brought report on her practical experience with this device in a practice of a primary care physician, including a case study. She  highlighted some of her good experience of dealing with sub­diagnostic problems as a general practitioner.

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Reports from Professional EventsInsights from the XIX Congress of Natural Medicine, Nitra, 16th–18th of October 2015

Teodor Rosinský, M. D., CSc.

Afternoon lectures of the first day of the congress were presented in two blocks. In  the first, more interdisciplinary tuned lecture, Ing.  Solár presented an overview of current views on the evolution of the universe and the solar system. Today physics hand in hand with astronomy, material chemistry and other natural sciences is currently in a state of fierce debate about the validity of the newly named theories and hypotheses, even if the final opinion is not yet on the agenda of professionals. However, it  is interesting for us to see the possible options for the interpretation of physical discoveries in such extent and thus their hypothetical relation to man and his psychic and somatic fundamentals.

Dr.  Rosinský devoted himself to semantics that is important for natural medicine, nature and naturalness. Nature as matter­ energy complex of a universe to multi­cosmic range can be considered as a tool to carry out tasks of naturalness as a basic property of individuality of sentient beings. In  that sense all medicine and certainly its natural component should respect  it, because any differences in the use of nature in a therapeutic sense and not respecting the naturalness of a patient may impair the effect. In addition it is also appropriate direction of the naturalness of the therapist with its awareness of the particularities of each individual meeting with the naturalness of the patient. The problem here is the inability of many languages semantically differ or even not at all to find terms that match the above pairs of words, well distinguishable in Slavic languages. In other, e. g. in English, but also in classical Greek there is only one expression and differences are therefore less clear and derivable only from the context.

Dr. Solárová presented a mathematical model of the genetic code in the Table I­Ching. Russian scientist Proff. Petukhov was first in the world

to calculate this model. The  author, however, presented probably as a world premiere the model, which, unlike the original was built not on the yang and yin characteristics in general, but  the yang and yin concatenation in pyramid model of acupuncture.

Ing. Solár followed up on the first lecture of this block with his lecture about the current point of view on the evolution of life. He  used not only biological but also physical and interpretative philosophical views of the various pro­ and anti­evolutionary directions. There was an interesting statement that the evolution law does not apply to the strongest, but  to most adaptive species, able to overcome sudden changes in the conditions of life. The ability of the cooperation within­ and interspecies plays an important role here.

Last Friday’s block was focused cytological and genetic. Ing. Kutejová talked about experimental research on the reaction of cells to stress. It was found that stress disturbs but not destroys the “peaceful” life of cell colonies in normal conditions, for many cell types respond promptly with metabolic changes, rearrangement of the colony structure and even conversion to spores. Experimental data show that functional cells in  vivo are capable to adequate response to the stress, even if several such “Plan B­s” limit functions of cells, and hence the body as a whole suffers. As it was a description of the results of the experiment, we can only derive the effects in the medical sense and even then the most powerful auto­ stressor – the patient’s own psyche has to be considered.

Mgr. Šímová gave an overview of new research in epigenetics compared with genetics long known. Interpretation approached generally less known internal processes and phenomena in genetic storylines, commonly understood mostly

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mechanically, in  fact, complex in information and therefore more difficult than  we, normally soothed by clarity of the genome, admit.

The  first day of the congress ended with a corporate lecture by Dr.  Králiková who familiarized participants with the use bio­dermal threads in treating pain.

The second day of the Congress began with a block dedicated to acupuncture. Dr. Mochnáč presen­ted his concept of modelling in acupuncture, which he has been dealing with. It  is based on mathematical procedures of arrangements of traditional trigrams and combinations of hexagrams as an expression of precisely defined natural phenomena and interpretations of thus assembled networks of phenomena known in acupuncture theory. All this serves as a basis both for a better understanding of the mechanisms of acupuncture and for a more accurate indication of process in acupuncture treatment.

The  experimental basis for acupuncture was brought by a team of workers from Faculty of Mechanical Engineering STU in Bratislava, led  by its Dean Assoc.  Prof.  Ing. Hučko. They used an active electro­acupuncture needle in an environment imitating the skin and subcutaneous tissue, the needle being heated at a temperature gradually rising from 40 to 60 °C and they measured isotherms produced around  it, which is how the temperature is transferred to the surroundings. Even though such experiments cannot be mechanically transferred to the processes in the human body, yet  their continuation and eventual gradual approach to any possible circumstances what actually happens in the body also by taking other parameters into account, for  example sound propagation and other measurable physical phenomena could

deepen our knowledge and reciprocally show more opportunities for direction of physicists’ and biophysicists’ research.

In Saturday’s second block workers from Russian Centre of Eye end Plastic Surgery made their presentations. First, prof.  Galiyakhmetov in authorship with prof.  Muldashev informed the participants about the actual workplace and in particular their discovery and a product called Alloplant. It  is made from dead people’s tissue by a complex procedure, which removes all the components that might cause the recipient of a transplant allergic or any defensive reactions. It is used not only as a material for transplantation, but  in small amounts in the form pharmaco­ puncture to a wide range of ocular disorders.

Then prof.  Musin reported on the use of  the mentioned preparation in degenerative musculo­skeletal diseases. The results of many application cases are very good, so there is the effort to expand the indications for use and also to improve the properties of this preparation. Both presentations also included sophisticated case reports and were significant and very beneficial enrichment of themes of Natural Medicine.

Next, afternoon block was devoted to issues of other somatic fields in presentations of domestic lecturers. Assoc.  Prof.  Dr. Bella gave a survey on natural medicine in oncology. He  greeted the Congress on behalf of the Director of the Institute of Oncology St. Elizabeth in Bratislava and his performance opens the way for broader cooperation of natural medicine and oncology. Since he founded his presentation on American materials of dividing of so­called alternative of complementary medicine, he  mentioned terms that have a different meaning in our region. He mentioned also in our region totally inacceptable typically American statements like enrolling hypnosis within such divided

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procedures, which is at  us, of  course already considerably more advanced and more recently seen as part of psychotherapy associated with long­term training and certification. However, he  mentioned also procedures that are not yet routinely introduced and therefore it was the inspiration for natural medicine in Slovakia.

Dr.  Hruška reported on his experience in dealing with gynaecological endometriosis in patients and except of clinical characteristics he innovatively elaborated considerations on parallel phenomena of loss of femininity in this disease. Subsequent phenomena of sterility were showed in a more integrated form than it is in the standard gynaecologic concept.

The report by Dr. Hurajova about homeopathy in otitis media was focused on case reports.

In  her precisely prepared report on IOP values after applying acupuncture Dr.  Miklošová pre­sented a very original and inspiring application of pyramid model of acupuncture on anatomical and physiological structures of the eye.

Saturday’s final block was focused truly interdisciplinary. Lecturers actually continued in elaborations of previous congresses, to complement the complex knowledge of relevant disciplines and thus further our knowledge of the impact of natural phenomena on  man. Assoc. Prof. Dr. Gajdoš dealt from a geophysical point of view with the impact of spatial, temporal and anthropogenic factors on the medical effects of electromagnetic fields, which are not negligible factors.

Prof.  Dr.  Baliak presented by spoken word but also pictures and video slope deformations as a major geofactor of environment and its effects on human.

Finally, Assoc.  Prof.  Dr.  Brindza focused on plants for the improvement and failing to improve nutrition, health and quality of life. Especially, however, he elaborated the still poorly understood both issues of biodiversity conservation, as well as the suitability and unsuitability of consumption of plants from other geographical and thus soil areas.

Last Sundays’ half­day congress took place in two blocks. In the first block dedicated to bioprocesses and bio products in a pair of presentations by Assoc.  Prof.  Dr.  Čambal et  al. and Dr.  Takáč et  al. authors were dealing with larval therapy of chronic non­healing wounds. They described various technical adaptations and procedures for the use of fly larvae to eliminate useless tissue from the wound and initiate the healing process. In  both reports they presented abundant and very well illustrated case studies.

The other two presentations were focused on the use of bee products. Dr. Zemanová et al. reported on the comparing of efficacy of natural bio­ honey to human microbial pathogens and  Dr. Biliková et  al. submitted the results of research on bee changing dietary protein to antibiotics. Papers provoked a very rich debate, including the present beekeepers or those liking honey.

Dr. Solár et al. presented vertical relations in the pyramid model of acupuncture. As  the world premiere they graphically illustrated the rotation of the octagram in its four states and precisely defined, what the characteristics of rotation of yin and yang  are. Thus in the same time they submitted further context that proves the correctness of pyramid model construction in acupuncture. Thanks to newly revealed context, this model is gradually still coming closer to a comprehensive understanding of acupuncture documents in traditional, instrumental and mathematical interpretation as uniform.

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Dr. Krausová familiarized those present with her experience with bio­ resonance in connection with acupuncture in routine practice.

Dr. Sázelová in an authorship with Dr. Lehocká dealt with the combination of conventional and unconventional medicine in the office of general practitioner also showing illustrative case studies.

The Congress was held traditionally in a very good social and working atmosphere. Through the variety of papers and genuine interdisciplinarity it was another step towards familiarizing the professional community and a source of new knowledge for participants. In  the name of all

congress participants may be cited from the final evaluation of prof. Galiachmetov, who spoke on behalf of our guests: “We  travelled around the world, we participated in a wide range of congresses, but we haven’t been in such a complex conceptual and integrating congress, where not only the body but also the soul are taken into account, and we will also participate on the next (next years’) congress.”

Of course, it was not possible to introduce all the procedures and directions of natural medicine, but maybe something that was not introduced yet appears in the following, the jubilee XX. Congress.

Teodor Rosinský, M. D., CSc.

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InformationsEditorial Policy

Editorial Policy

The magazine “Akupunktúra a naturálnamedicí­na” (Acupuncture and Natural Medicine) is  an online bi­monthly journal published by “Lekárska spoločnosť naturálnej medicíny” (Slovak Medical Society of Natural Medicine). The scope of the magazine is the study of energy­information related processes in physiology and pathology and environmental and social effects on humans from the perspective of natural medicine, mainly acupuncture. The  magazine publishes contemporary peer­reviewed articles on theory, clinical practice of acupuncture and other medical fields, natural, technical and social sciences in accordance with the interdisciplinary focus of the magazine. The magazine is published in Slovak and English.The  publisher and the editorial staff are not responsible and may not be held responsible in any way for any damages, loss or other negative consequences arising directly or indirectly from any potential improper application of the medical and treatment methods, techniques and protocols published herein and may neither be held liable criminally or otherwise for any damages, loss or other negative consequences arising from any potential breach of provisions of the law by authors of articles or contractors of advertising. The  author and or the contracting party are solely responsible for the content of text, advertising, photos and images. The  publisher reserves the right to allocate advertising at his own discretion unless previously agreed with the contracting party otherwise. The  holder of the license to publish the magazine “Akupunktúra a naturálnamedicína” (Acupuncture and Natural Medicine) is “Prvá klinika akupunktúry a naturálnej medicíny G.  Solára s. r. o.” (G.  Solar’s First clinic of acupuncture and natural medicine, ltd.).

Open AccessThe magazine “Akupunktúra a naturálnamedicína” (Acupuncture and Natural Medicine) prefers open access to information, e.g. all  articles published in the magazine are available online at  www.naturalnamedicina.com immediately upon publishing.

Rights and PermissionsThe  entire content of the magazine is freely available for non­commercial use only. The  content of the magazine may be copied and/or distributed and/or information contained therein be used as a part of other work(s) only under the condition that the original source is properly cited.The  author is responsible for the entire content of the article including any rights of third parties (such as copyrights  etc.). The  author warrants that the article has not been previously published in any way. By sending the article to the publisher of the magazine, the  author gives the publisher his irrevocable consent with permanent publishing of such article in the magazine and without entitlement to compensation and also gives his consent with the processing of personal data. Pursuant to provisions of the Act no. 618/2003 Coll. as amended, the author does not reserve the right to give consent with copying and public transmission of such article, not affecting other rights of the author granted by the above mentioned Act.The  editorial staff reserves the right to make minor linguistic and graphical changes to the article shall the need arise. The  editorial staff shall at its own discretion decide on which issue and what section of the magazine the article will be published  in. Articles and contributions not specifically requested by the editorial staff are not returned to the author.

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InformationsInstructions for Authors

Instructions for Authors

E-magazine Acupuncture and Natural Medicine publishes following types of contributions:

Peer Reviewed Contributions1. Study  – original scientific work based on

fundamental research, comprehensible scientific manuscript analysing relevant results. We are not interested in manuscripts with the conclusion “the  authors confirmed the results of other authors”.

2. Survey  – may  deal with actual topics and issues in acupuncture and other interdisciplinary fields, informative and discussion contributions. A survey should not be a review of an existing literature, it should bring a new perspective on the problem.

3. Case report – will be published only if it brings new and original view on a topic and contains innovative findings.

Editor Reviewed Contributions4. Review of a scientific work – short informative

review. It  is necessary to state names of authors, the original and the translated title of the reviewed publication and its ISBN/ISSN, the  year of publishing and in the frame of a review its asset.

5. Report on scientific events – should contain the name of the event, its  place and date, the  organizer of the event, an  overview of contributions and discussion eventually short abstracts of presentations and a total evaluation of the event by the author of the report inclusive its possible asset.

6. Discussion to articles published in the e-magazine Acupuncture and Natural Medicine. It  is necessary to state the title of the article, the  number and volume of the magazine Acupuncture and Natural

Medicine that is discussed, the title, the name, the workplace and the function of the person discussing.The Editorial Board reserves the right not to publish a discussion paper to the published article. Author of the discussed article has a right to react to every discussion as the first. The  discussion and the reaction will be published concurrently in the same issue of the magazine Acupuncture and Natural Medicine.

7. Other papers connected to acupuncture and natural Medicine.For  articles with a nature of essays and reflections and work that doesn’t require a peer­review there is a section An Insight into the Acupuncture Thinking reserved.

The Form of Studies and SurveysEvery manuscript should contain a letter to Editor, in which the corresponding author states that he/she wishes to submit the manuscript to the e­magazine Acupuncture and Natural Medicine for consideration. He  also states, that there is no conflict of interest, and that all authors have read the manuscript being submitted and the editorial policy and they agree with it as well as with submitting the manuscript. Editors are not responsible for legal consequences in case of violation of legal, ethical and other provisions.

The Author Is Obliged to Deliver the Following Items:• names and contacts to all authors;• one author designated as corresponding

author;• e­mail address;• full postal address;• phone number;• all necessary files have been uploaded;• key words;• all figure captions;

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InformationsInstructions for Authors

• all tables and illustrations (including title, description, footnotes);

• manuscript has been “spellchecked”;• references are in the correct format;• all references mentioned in the Reference List

are cited in the text and vice versa;• permission has been obtained for use of

copyrighted material from other sources (including the Web), if they were used;

• it is necessary to state the grant support and a statement that there is no conflict of interest.

Basic FormatMS  Word  2003 compatible version (file format *.doc) Author submits the whole manuscript in English and native language.

Structure of the Article1. Title of the contribution, name and surname

of the author(s) (including titles).2. Summary  – a short content of the contri­

bution.3. Key Words – 4–6 key words, phrases4. Introduction  – state the objectives of the

work and provide an adequate background, avoiding a detailed literature survey or a summary of the results.

5. Materials and methods  – Provide sufficient detail to allow the work to be reproduced. Methods already published should be indicated by a reference; only relevant modifications should be described. In studies that need an informed consent or an approval by the ethics committee these have to be explicitly stated.

6. Results should be presented precisely using adequate scientific methods. Don’t duplicate detailed information from tables and pictures. Include graphs under pictures (for  example: Picture 5: Graph of dependence…).

7. Discussion should directly relate to the study being reported and should include a confrontation with references. Do not include a general review of the topic.

8. Conclusion – a brief summary of the results, hypotheses and objectives

9. Acknowledgements provides a space to acknowledge colleagues who have assisted or collaborated in the study who are not listed as authors.

10. Contact to author(s): name and surname of author(s) (including titles), institution, work contact. Author for correspondence: name and surname, mailing address, e­mail and eventually phone number to the workplace.

11. References should be cited in text by number and numbered in order they are cited.

Format of Listed References• Surname, N. (initial and the name after

comma), several authors separated by a comma. In case of more than 3 authors, state names of first 3 authors and use a shortening “et  al.” behind the last name. The  delimiter between names of authors and the title of the contribution is a colon.

• The title have to be written as it is in the source. The translated title of the article may be stated in the square brackets.

• The delimiter between the title of the contribution and other information is a dot.

• With the exception of the first edition of the information about the order of the issue, its changes and amendments is mandatory.

• Place of publication is stated in the original language, the  name of the publisher may be written as indicated in the source.

• Date of issue is required.• The range  – in printed monographs infor­

mation about the range are stated by the number of pages (90  p.) or  by number of volumes (vol. 3) if there are more volumes of the monograph.

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InformationsInstructions for Authors

• ISBN  – this information is required if re­ferred to a monograph as a whole. It  is not mandatory only in cases of referring to parts of documents, contributions in monographs and papers in magazines.

• Part of a document – in case of referring to a contribution in a monograph, an article in an almanac or in a magazine information about the monograph, almanac or magazine should follow after a conjunction “In:”.

Pictures and photographs in an appropriate quality should be submitted as separate files. Eventual descriptions should be submitted in text format. All  authors wishing to use illustrations already published must first obtain the permission of the author and publisher and/or copyright holders and give precise reference to the original work. This permission must include the right to publish in electronic media.

Tables – in case of inserted tables and/or graphs from MS Excel program it is necessary to submit the original file. Number tables consecutively in accordance with their appearance in the text. The  text should include references to all tables. Tables should be marked by a title above and any notes below. The language used in a table must be identical with the language of the contribution.

Language Correction of ContributionsAuthor is responsible for the language and the content of the contribution. Eventual correc-tions by the editor are possible only in the case

of spelling errors and editing typography. If necessary, the editorial board reserves the right to request an additional proofreading by the author. One set of proofs will be sent by e­mail to the corresponding author. Editorial board ensures the article to be published in the next appropriate issue. Therefore, it  is important to ensure that all of your corrections are sent back to us in one communication. Subsequent corrections will not be possible, so  please ensure your first sending is complete. Proof must be returned within two days; late return may cause a delay in publication. Please check text, tables, legends, and references carefully.

A study is subject to all instructions for authors.

A survey is subject to following items: abstract, key words, introduction, core, conclusion, con-tacts on authors and references.

A  case report is subject to all instructions for authors.

Please Submit the Manuscript as Follows:Authors from Slovak republic and Czech republic:Executive editor: G. Solár, M. D., PhD.,[email protected]­in­chief: S. Sázelová, M. D.,[email protected] from other countries (native language and English version):Associate editor responsible for foreign affairs: E. Baumann, M. D., [email protected]

ISSN 1339‑4703

Acupuncture and Natural Medicine 5–6/2015