50

HPLC Accessories

Start-Up Kit, analyticalOrdering Information

Description

OrderNo. Article QuantityA0910 Start-Up Kit, analytical 1

Consists of:A0131 Capillary, 1/16” AD, 0.25mm ID, stainless steel 3 mA0132 Capillary, 1/16” AD, 0.5mm ID, stainless steel 3 mA0133 Capillary, 1/16” AD, 0.75mm ID, stainless steel 3 mA0129 Set of precut capillaries, 1/16” 8A0137 Capillary cleaning set 1A0118 Low dead volume coupling of 2 capillaries, 1/16”, stainless steel 5A0112 Bushings for capillaries, 1/16”, stainless steel 10A0110 Ferrules for capillaries, 1/16” 30A0146 Caps, 1/16”, polymer 1A0120 T-connector for 3 capillaries, 1/16”, stainless steel 1A0152 PTFE tubing, 1/16”, 0.45mm ID 3 mA0153 PTFE tubing, 2mmx1.4mm ID 3 mA0108 Set of DYNASEAL connection system, short 2A0181 Set of DYNASEAL connection system, long 1A0726 Injection syringe, 100µl 1A0809 Pliers for capillaries 1

This kit is recommended when orderingan analytical HPLC system for the firsttime.

Weight0.5 kg

Start-Up Kit, biocompatibleOrdering Information

OrderNo. Article QuantityA1039 Start-Up Kit, biocompatible 1

Consists of:A0685 Capillary, PEEK, 1/16”, 0.25mm ID 1.5 mA0691 Capillary, PEEK, 1/16”, 0.5mm ID 1.5 mA0692 Capillary, PEEK, 1/16”, 0.75mm ID 1.5 mA0137 Capillary cleaning set 1A0149 Low dead volume coupling of 2 capillaries, 1/16”, polymer 5A0585 Bushings for capillaries, 1/16”, short, PEEK 10A0139 Ferrules for capillaries, 1/16”, PEEK 30A0146 Caps, 1/16”, polymer 10A0150 T-connector for 3 capillaries, 1/16”, polymer 1A0152 PTFE tubing, 1/16”, 0.45mm ID 3 mA0153 PTFE tubing, 2mmx1.4mm ID 3 mA0108 Set of DYNASEAL connection system, short 2A0181 Set of DYNASEAL connection system, long 1A0726 Injection syringe, 100µl 1A0569 Tube cutter 1

Description

This kit is recommended when orderingan analytical, biocompatible, and inertHPLC system for the first time.

Weight0.2kg

51

Start-Up Kit, preparativeOrdering Information

Description

Recommended when ordering a prepa-rative HPLC system for the first time.

Weight0.9 kg

Description

Recommended for sample preparation.

OrderNo. Article QuantityA1104 Start-Up Kit, preparative 1

Consists of:A0639 Capillary, 1/8”, 1.6mm ID 1.5mA0640 Capillary, 1/8”, 2.2mm ID 1.5m A0134 Capillary, 1/16”, 1.0mm ID 3 m A0873 PTFE tubing, 1/8”, 2mm ID 3 m A0732 PTFE tubing, 1/8”, 1.5mm ID 3 m A0154 PTFE tubing, 4mm OD, 3mm ID 3 m A0737 Capillary, 1/8”, PEEK, 1.6mm ID 1.5mA0478 Tefzel tubing, 1/8”, 1.6mm ID 3 m A0644 DYNASEAL connection system for capillaries 1/8”, stainless steel 1A0830 Bushing for capillaries 1/8”, stainless steel 1A2511 T-connector for 3 capillaries 1/8”, short, stainless steel 1A2512 Low dead volume coupling for 2 capillaries 1/8”, stainless steel 5A2513 Low dead volume coupling for capillaries 1/16”<–> 1/8”, stainless steel 1A0117 Low dead volume coupling for 2 capillaries 1/16”, stainless steel 1A0736 Set of DYNASEAL connection systems, 1/8”, long 1A0232 Sealing ring for capillaries 1/8”, polymer 10A0874 Ferrules for capillaries 1/8”, stainless steel 10A1239 Split-grooved clamping ring for capillaries 1/8” 4X0003 Set of two open-end wrenches, wrench caliber 1/4” and 5/16” 2X0030 Open-end wrench, wrench caliber 8/10” 2A0573 Luer-Lock syringe, glass, 10ml 1A0734 Caps, 1/8”, polymer 10

Filters for Sample PreparationOrdering Information

OrderNo. Article QuantityA0950 All glass vacuum unit 1A0951 PTFE membrane filter, 47mm ∅ , pore size 0.45µm 100A0917 Membrane filter made out of regenerated cellulose,

47mm ∅ , pore size 0.45µm 100

52

HPLC Accessories

Sample Loops, analyticalOrdering Information

Description

OrderNo. Sample Loops QuantityA1159 1/8’’, volume up to 11ml 1A1160 1/8’’, volume up to 45ml 1A1054 1/16”, volume up to 11ml 1A1055 1/16”, volume up to 45ml 1A1043 1/8”, volume up to 1ml, stainless steel 1A1110 1/8”, volume up to 1ml, PEEK 1A1044 1/8”, volume up to 2ml, stainless steel 1A1111 1/8”, volume up to 2ml, PEEK 1

Sample Loops, preparativeOrdering Information

Injection SyringesOrdering Information

Sample Loops, stainless steelOrderNo. SampleVolume QuantityA0331 5 µl 1A0561 10µl 1A0562 20µl 1A0836 30µl 1A0563 50µl 1A0837 75µl 1A0564 100µl 1A0565 200µl 1A0566 500µl 1A0567 1,000µl 1A0568 2,000µl 1

OrderNo. SampleVolume QuantityA0723 10µl 1A0724 25µl 1A0725 50µl 1A0726 100µl 1

OrderNo. SampleVolume QuantityA0727 250µl 1A0728 500µl 1A0729 1,000 µl 1A0730 2,500 µl 1

Sample Loops, PEEKOrderNo. SampleVolume QuantityA1058 10µl 1A1059 20µl 1A1060 50µl 1A1061 200µl 1A1057 500µl 1

Loop Filling PortOrderNo. Article QuantityA0555 Loop filling port 1

Description

KNAUER’s sample loops are suitable forall types of HPLC systems. Sample loopsmade out of PEEK will be delivered withour DYNASEAL connection system forcapillaries 1/16”.

Individual sizes available upon request.

We recommend using the loop fillingport if the injection syringe cannot be inserted into the injection port.

Suitable for 1/16” and 1/8” connections.Useful for full and partial loop filling.The 11ml and 45ml sample loops consist of an empty stainless steel column with an internal piston. Low peak broadening. Applicable forflow rates exceeding 1ml/min.

Description

Glass syringes are suitable for all stan-dard HPLC injection valves.

53

Capillaries, stainless steelOrdering Information

Description

OrderNo. OD (inch) ID (mm) Length (cm) QuantityA0130 1/16” 0.10 300 1A0131 1/16” 0.25 300 1A0132 1/16” 0.50 300 1A0133 1/16” 0.70 300 1A0134 1/16” 1.00 300 1A0123 1/16” 0.10 10 10A0124 1/16” 0.10 20 10A0125 1/16” 0.10 30 10A0126 1/16” 0.25 10 10A0127 1/16” 0.25 20 10A0128 1/16” 0.25 30 10A0639 1/8” 1.60 150 1A0640 1/8” 2.20 150 1

Resistant capillaries made out of stain-less steel for all HPLC connections.

Capillaries, PEEKOrdering Information

OrderNo. OD (inch) ID (mm) Length (cm) QuantityA0685 1/16” 0.25 150 1A0691 1/16” 0.50 150 1A0692 1/16” 0.70 150 1A0737 1/8” 1.60 150 1

Description

Suitable for biochromatography. Metal-free PEEK capillaries consist ofpolyether-ether-ketone that show apressure stability of up to 400bar depending on the inner diameter.Don’t forget to take advantage of ourDYNASEAL connection system whenworking with PEEK capillaries.

Tools for Capillaries, stainless steelOrdering Information

OrderNo. Article QuantityA0137 Capillary cleaning set 1A0809 Pliers for capillaries 1

Description

Tools to keep your capillaries in »shape«.

54

HPLC Accessories

Tefzel TubingsOrdering Information

OrderNo. OD (inch) ID (mm) Length (cm) QuantityA0182 1/16” 0.25–0.30 300 1A0183 1/16” 0.70 300 1A0478 1/8” 1.60 300 1

Description

Tube Cutter, Ferrules, and Sealing RingsOrdering Information

OrderNo. Tube Cutter QuantityA0569 Tube cutter 1

OrderNo. Ferrules for capillaries QuantityA0110 1/16”, stainless steel 30A0111 1/16”, stainless steel 100A0874 1/8”, stainless steel 10

OrderNo. Sealing Rings for capillaries QuantityA0139 1/16”, polymer 30A0140 1/16”, polymer 100A0232 1/8”, polymer 10A1062 1/16”, PEEK 10A1063 1/8”, PEEK 10

Tefzel tubings consist of perfluorinatedhydrocarbons. They are flexible, inert,and gas tight. Pressure stability reachesup to 200bar depending on the innerdiameter.

Description

Cuts all flexible plastic tubes, particu-larly suitable for capillaries made out ofPEEK.

OrderNo. OD (inch) ID (mm) Length (cm) QuantityA0152 1.6 (1/16”) 0.45 300 1A0153 2 1.45 300 1A0732 3.2 (1/8”) 1.5 300 1A0873 3.2 (1/8”) 2.00 300 1A0154 4 3.00 300 1

PTFE TubingsOrdering Information

Description

PTFE tubings consist of perfluorinatedhydrocarbons. They are flexible, bio-compatible, and gas tight.

55

Couplings, T-Connectors, and Cross ConnectorsOrdering Information

Description

All parts made out of polymer consist of PETP (Arnite) if not mentioned otherwise.

Low Dead Volume Couplings, stainless steelOrderNo. for connecting: QuantityA0117 2 capillaries 1/16” 1A0118 2 capillaries 1/16” 5A0119 2 capillaries 1/16” 25A2512 2 capillaries 1/8” 5A2513 2 capillaries 1/16”<–> 1/8” 1

Low Dead Volume Couplings, polymerOrderNo. for connecting: QuantityA0148 2 capillaries, 1/16” 1A0149 2 capillaries, 1/16” 5

T-Connector, stainless steelOrderNo. for connecting: QuantityA0120 3 capillaries 1/16” 1A2511 3 capillaries 1/8” 1

T-Connector, polymerOrderNo. for connecting: QuantityA0150 3 capillaries 1/16” 1

Cross-Connector, stainless steelOrderNo. for connecting: QuantityA0121 4 capillaries 1/16” 1A1096 4 capillaries 1/8” 1

Cross-Connector, stainless steelOrderNo. for connecting: QuantityA2514 3 capillaries of 1/16” and 1 capillary of 1/8” 1

Cross-Connector, polymerOrderNo. for connecting: QuantityA0151 4 capillaries of 1/16” 1

56

HPLC Accessories

Bushings and Caps (Blind Fittings)Ordering Information

Description

OrderNo. Bushings for Capillaries QuantityA0112 1/16’’, short, UNF 10/32, SW 1/4’’, stainless steel 10A0113 1/16’’, short, UNF 10/32, SW 1/4’’, stainless steel 25A0115 1/16’’, long, UNF 10/32, SW 1/4’’, stainless steel 3A0116 1/16’’, long, UNF 10/32, SW 1/4’’, stainless steel 10A0830 1/8’’, M8x1, SW 10, stainless steel 10A0141 1/16’’, short, UNF 10/32, knurled, polymer 10A0142 1/16’’, short, UNF 10/32, knurled, polymer 30A0143 1/16’’, short, UNF 10/32, knurled, polymer 100A0144 1/16’’, long, UNF 10/32, knurled, polymer 10A0145 1/16’’, short, UNF 10/32, with double-cone sealing,

knurled, polymer 10A0733 1/8’’, short, UNF 10/32, M8 x 1, with sealing ring,

knurled, polymer 10A0584 1/16’’, UNF 10/32, PEEK 10

OrderNo. Caps (blind fittings) QuantityA0146 1/16’’, short, UNF 10/32, polymer 10A0147 1/16’’, short, UNF 10/32, polymer 30A0734 1/8’’, M8x1, polymer 10A0582 1/16’’, short, UNF 10/32, knurled, polymer 10

DYNASEAL Connection SystemOrdering Information

Description

All bushings have inch threading (UNF)and are available with short and longheads. The long head bushings are recommended when tight connectionsare required.Ferrules are slipped over the capillary,deformed during tightening, and can-not be removed afterwards. Thus, theconnection is sealed.

OrderNo. Bushings for Capillaries QuantityA1021 1/16”, short 10A1064 1/16”, long 5A1067 1/8”, short 4A0735 1/8”, long 4

OrderNo. Split-Grooved Clamping Rings QuantityA0484 1/16” 4A1239 1/8” 4

OrderNo. Sealing Rings QuantityA0139 1/16”, polymer 30A0140 1/16”, polymer 100A1062 1/16”, PEEK 10A0232 1/8”, polymer 10A1063 1/8”, PEEK 10

OrderNo. Double-Cone Sealings QuantityA1022 1/16”, polymer 10A1070 1/16”, PEEK 10

57

DYNASEAL Connection System Ordering Information – System –

Description

Important Note

The DYNASEAL connection system connects capillaries made out of stain-less steel and PEEK, as well as PTFE andTefzel tubings with saftey and low deadvolumes. It allows maintenance-freeoperation and provides a long life. Suitable for UNF-threads of type 10/32.

The system consists of a bushing, a split-grooved clamping ring, and a polymersealing. The split-grooved clampingring and polymer sealing are slippedover the capillary »back to back«, whilethe bushing tightens all parts. Thus, leak-free operation is made possible.DYNASEAL connections are pressurestable up to 450bar.

DYNASEAL can be optionally used withdouble-cone sealings made out of PEEK or stainless steel. In this case, pressure stability is accordingly reduced to 150bar(PEEK) and 350 bar (polymer).

DYNASEAL is adaptable in new con-ditions such as variable bore depths ordetector connections because it is mobile and reuseable.

The DYNASEAL connection system is available for capillaries with 1/16” (analytical HPLC) and 1/8” (preparativeHPLC).In general, to connect KNAUER, Rheo-dyne, or other injection systems; thelong head bushings can be deliveredupon request.

OrderNo. Article QuantityA0108 1/16”

Short head bushings 4Split-grooved clamping rings 4Sealing rings 8

A0181 1/16”Long head bushings 3Split-grooved clamping rings 3Sealing rings 4

A1020 1/16”Short head bushings 10Double-cone sealings 10

A1069 1/16”Long head bushings 5 Double-cone sealings 5

A0736 1/8”, M8x0.1Long head bushings 4Split-grooved clamping rings 4Sealing rings 8

A0644 1/8”, M8x1Short head bushings 4Split-grooved clamping rings 4Sealing rings 8

58

HPLC Accessories

Column Inlet FiltersOrdering Information

Description

The Column Inlet Filter can be installedbetween the injection system and thecolumn. Column inlet filters protect the column from particle contaminationcaused by sealing material (i. e. pumppistons). We recommend adding a filterafter the column. This prevents conta-mination of the detector cell. Inlet fil-ters are available in stainless steel andPEEK.

OrderNo. Article QuantityA0109 Column Inlet Filter, stainless steel 1A0015 Set of spare parts for A1090, includes:

6 sieves (3µm), 6 sieves (7µm), 12 glass filters, 6 PTFE gaskets 1A0138 Set of open-end wrenches, wrench caliber 1/4” and 5/16” 2A0573 Luer-Lock glass syringe, 10µl 1A0653 Luer-Lock glass syringe, 20µl 1

Ready-to-use filtration units for ultra-purification of small sample volumes.The chemically resistant polypropylenehousing has been designed to achieve a maximum sample yield with a high retention time. Backflow/overflow ofthe filtrate is effectively avoided withelongated Spike and Luer outlets.

Syringe Filtration UnitsOrdering Information

Description

Polypropylene-reinforced PTFE (hydrophobe)Pore Size Dead Volume � Area

OrderNo. Article µm µl mm cm2 QuantityA1258 Minisart SRP4 0.45 1 4 0.10 50A1259 Minisart SRP4 0.45 1 4 0.10 500A0958 Minisart SRP15 0.45 10 15 1.70 50A1264 Minisart SRP15 0.45 10 15 1.70 500A0953 Minisart SRP15 0.20 10 15 1.70 50A0954 Minisart SRP15 0.20 10 15 1.70 500A0956 Minisart SRP25 0.20 100 25 4.80 50A0957 Minisart SRP25 0.45 100 25 4.80 50A1262 Minisart SRP25 0.45 100 25 4.80 500

Cellulose (hydrophile)Pore Size Dead Volume � Area

OrderNo. Article µm µl mm cm2 QuantityA1260 Minisart RC4 0.45 5 4 0.07 50A1261 Minisart RC4 0.45 5 4 0.07 500A1265 Minisart RC15 0.45 10 15 1.70 50A0960 Minisart RC15 0.45 10 15 1.70 500A0961 Minisart RC25 0.20 150 25 4.80 500A1263 Minisart RC25 0.45 150 25 4.80 50A0962 Minisart RC25 0.45 150 25 4.80 500