Accelerate Your Research
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Transcript of Accelerate Your Research
Primary Human Biospecimens
Diseased and Healthy Whole Blood
Processed Blood Derivatives
Dissociated Tumor Cells (DTCs)
Formalin-Fixed Parrafin Embedded Tissue Blocks (FFPE)
Flash Frozen Tissue Blocks
Matched Blood and Tissue
Your Quality Source For:
"Conversant provides premium quality primary human samples along with detailed information on patients in a timely fashion. We routinely observe >85% cellular viability from the thawed primary samples obtained from both cancer and non-cancer patients."
9001 Certified
-Obdulio Piloto, PhD, Research Scientist, Exelixis, Inc.
Blood Products
CheckPoint Inhibitor/Immunotherapies
Mutation Characterized Blood
Healthy Donors/Normal Controls
Opdivo, KeyTruda, Yervoy, and Tecentriq Treated Patients
PD-1, PD-L1, CTLA4 Inhibitor
Circulating Biomarkers for Immunotherapy
Longitudinal Collections
Outcome Data/Responders vs. Non-Responders
Longitudinal Collections
Known HLA. Confirmed HLA-A02. *Tested via NGS sequencing with 6 digits on the allele
Growing and Diverse Donor Pool (Age/Race/Gender/Time Matched)
Virus Tested
Custom Processing and Cell Isolations
At Conversant Bio, we offer a wide variety of human peripheral blood samples
from a number of different indications, including normal patient populations.
We also provide patient annotation for our blood samples including age, race,
gender, smoking history, alcohol history, primary diagnosis, treatment history,
known mutation statuses, and more.
Early and Late Stage Solid Tumors
Non-Small Cell Lung Cancer
Colorectal Cancer
Prostate Cancer
Ovarian Cancer
Breast Cancer
Melanoma
Pancreatic
More
Clinically Observed Mutations (Known Variants)
EGFR
KRAS
ALK
MET
ERBB2 (HER2)
RET
HRAS
TP53
BRAF
NRAS
Known Wild Types
More
Double-Spun Plasma
Processing Let our experienced team handle all of the complex processing, so you can
focus on the important things. Our standardized double-spin protocol has been
optimized to provide the highest yields of cell-free DNA with low gDNA
contamination. We preserve both the buffy coat and clarified plasma for analysis
of unique somatic mutations.
Cell-Free Nucleic Acid Protocol Whole blood collected in Conversant Bio provided Streck Cell-Free DNA BCT®.
Initial spin at 1,500 x g for 10 minutes to separate blood fractions.
Carefully aliquot plasma fraction into nuclease-free tube. Save buffy coat.
Spin isolated plasma fraction at 16,000 x g for 10 minutes to yield a
supernatant plasma free of excess cellular debris and gDNA contamination.
"To remove possible gDNA contamination from white blood cells, we need the
blood samples to be collected in Streck and spun twice and the speed for the
second spin is 16,000 x g for 10 mins. Our analysis would be very sensitive to the
contamination."
About Streck Cell-Free DNA BCT® Cell-Free DNA BCT® is a blood collection tube with a formaldehyde-free
preservative stabilizing nucleated blood cells. This unique stabilization prevents
the release of genomic DNA, allowing isolation of high-quality cell-free DNA
which can be further used for a wide range of downstream applications in
clinical research studies, drug discovery and diagnostic assay development.
Cell-Free DNA BCT® reduces the need for immediate plasma preparation due to
its unique stabilization properties. Cell-Free DNA is stable for up to 14 days,
while circulating tumor cells are stable for up to 4 days, at room temperature,
allowing convenient sample collection, transport and storage.*
Nucleic Acid Extractions
Platelet-Rich Fractions (Tumor-Educated Platelets)
Circulating Tumor Cells
Exosomes
Cell Isolations
Customized protocols available as well:
-Senior Scientist, Major U.S. Diagnostic
Cell-Free DNA BCT® is registered copyright of Streck® * Claims made by Streck® https://www.streck.com/product.aspx?p=Cell-Free%20DNA%20BCT
Dissociated Tumor Cells
How are these tumors processed? - Dissociated Tumor Cells are obtained by processing resected
tumor tissue using both enzymatic and mechanical techniques. The goal is to preserve cell
surface markers while maintaining viability and cell counts for the diverse cell population
within each tumor. Most of our R&D in 2015 was geared towards customizing protocols (by
disease indication) to ensure maximum cell viability. For this reason, we consider our exact
dissociation protocol methods to be proprietary.
Why use dissociated tumor cells? - Tumor tissues are a critical component of many oncology
research projects. However, fresh tissues are increasingly harder to identify, accrue, ship,
process, and store. Conversant Bio offers Dissociated Tumor Cells to researchers looking for a
source of high-quality primary cells, without the hassle of fresh tissue accrual.
We currently have 2,000+ aliquots in stock from over 200 unique resections. Each aliquot has
over 1 million cells and >50% cell viability.
20
40
60
80
Colorectal
Ovarian
Endometrial
Kidney
Melanoma
Lung
0
Unique Dissociated Tumor Cell Patients Available
“We are clearly able to detect viable myeloid populations in the RCC DTCs. There appears to be
fairly similar distributions across the three samples which is great. We’re showing T cell, B cell,
NK, Monocyte and Neutrophil percentages of CD45+ Viable cells.“
-Researcher, Clinical-Stage Therapeutics
Surgical Tissue
All blocks were from US-sourced, patient cases confirmed by two U.S. Board Certified Pathologists. Six
5-micron unstained tissue slides per case were supplied. One slide was H&E stained and reviewed by
a CLIA Lab pathologist. The pathologist circled the most appropriate area of tumor tissue for testing
and estimated the tumor density within the circled region. Tissue from unstained slides was
microdissected using the H&E stained slide as a guide. DNA was then extracted from the dissected
tissue using one of two chemistries, depending on the quantity of tumor tissue available for testing.
Spectrophotometric analysis of the optical density (O.D.) at 260 and 280 nm was performed to
determine the quality and quantity of the extracted nucleic acids.
Surgical Tissue
Conversant Bio's FFPE tissue bank is designed to meet your quality needs. We have thousands of
pathologist reviewed archival tissue blocks from US surgical cases available in our biorepository.
Our samples were specifically selected to aid in discovery, validation, and controls of both antibody
and molecular testing to help you deliver on your project.
Formalin Fixed Paraffin Embedded Tissues
Mutation Testing Process
We offer ALK, cMET and MSI cases. Other mutations available include:
Mutation Characterized FFPE
"We found that using Conversant Bio-sourced FFPE blocks increased the
success rate of our research by 40%. Their quality saves us time and money."
-Biomarker Scientist, International Biotechnology Firm
Lung - EGFR and KRAS
Breast - PIK3CA
Colorectal - KRAS, BRAF and NRAS
Melanoma - BRAF and NRAS
We have 1000 positive cases across all of these mutations, including
many that are wild type.
DTCs and PBMCs are matched pre-surgical blood and treatment naive tumor tissue. The tumor is
processed to single cell tumor suspension and blood is processed to mononuclear cells.
These biospecimens are ideal for researchers who are looking to study immune cells within the tumor
and identify ways to activate immune response to attack tumor cells. These sets allow for a comparison
of the immune population in the tumor and the immune population in the peripheral blood.
Matched Sets
FFPE and Double-Spun Plasma
The Process 1. Blood collected just before surgical resection in EDTA tubes.
2. Blood is double-spun within two hours of collection and plasma is
stored at negative 80 degrees Celsius.
3. Plasma is then matched with FFPE resected tumor tissue.
Melanoma, Renal Cell Carcinoma, Colon, Ovarian, and Lung Cancers
All Samples are Pre-Treatment
All Stages Represented
Dissociated Tumor Cells and Peripheral Blood Mononuclear Cells
Patient Profiles Data ProvidedBase Demographic Data
Pathology Report
Current Treatment
Block QC Data
Contact Us Today [email protected]
866-838-2798
Conversant Bio 601 Genome Way, Suite 1200
Huntsville, AL 35806 (866) 838-2798
www.conversantbio.com
Prostate Cancer
Breast Cancer
Non-Small Cell Lung Cancer
Small Cell Lung Cancer
Colorectal Cancer
Melanoma
Pancreatic Cancer
Head and Neck Cancer
Bladder Cancer
Kidney Cancer
Liver Cancer
Glioblastoma
Ovarian Cancer
Gastric Cancer
CML/CLL
Oncology Autoimmune
Hematology
Crohn’s
Ulcerative Colitis
Systemic Lupus Nephritis
(SLE)
Rheumatoid Arthritis
Sjogren's
Psoriasis/Psoriatic Arthritis
AML/ALL
Multiple Myeloma
MGUS/MDS
Hodgkin’s and NHL
Lymphomas
Healthy Donors Age/Race/Gender
Matched Controls
Known HLA
Virus Tested
OtherPulmonology
Neurology
Cord Blood
More
Primary Sample Indications