ACB Kale Protein Blend - Active...
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ACB Kale Protein Blend Tomorrow’s Vision… Today! ® Moisturizing + Film-Forming + Nourishing + Conditioning
Transcript of ACB Kale Protein Blend - Active...
ACB Kale Protein Blend
Tomorrow’s Vision… Today!®
Moisturizing + Film-Forming + Nourishing + Conditioning
ACB Kale Protein Blend
Technical Information:
Product Code: 20036
INCI Name: Hydrolyzed Kale Protein & Hydrolyzed Carrot Protein & HydrolyzedLemon Protein
INCI Status: Proposed
Suggested Use Levels: 1.0–10.0%
Suggested Applications: Moisturizing, Film-Forming, Nourishing, Conditioning
Kale
Health Benefits:
Vitamin A and Eo Increases hydrationo Increases elasticityo Boosts collagen production
Vitamin C o Helps make the collagen needed for skin strengtho Antioxidant
Carrots
Health Benefits:
Antioxidantso Protect and heal while also providing anti-aging benefits to skin
Vitamin A (beta-carotene)o Most advantageous antioxidant packed into this vegetableo Provides protection from damage-causing free-radicals o Nourishes overly photo-exposed skin and plays an essential role
in skin maintenanceo Aids in repairing skin damageo Helps to balance and hydrate the skin’s cells to alleviate dry,
chapped or scaly skin
Lemons
Health Benefits:
The Juice• Source of Alpha Hydroxy Acid (AHA) citric acid
o Typically contains between 5 and 8 percent citric acid• Vitamin C
o Fresh lemon juice has been recommended in some herbals as a treatment for sunburn
• Natural skin lightenero Relatively high acid content, coupled with antioxidant activity
The Peel• Antioxidant properties• Anti-tyrosinase activity
o Balances skin tone
In-vivo Efficacy Data: ACB Kale Protein Blend
Protocol:
Materials & Methods:
2.0% ACB Kale Protein Blend vs. a control shampoo
2.0% ACB Kale Protein Blend vs. a control conditioner
Sensory evaluation was conducted for baseline, participants rated hair 1-10
½ Subject’s head washed with control, ½ washed with ACB Kale Protein Blend
Subject’s hair was dried using round brush and hair dryer
Sensory evaluation was conducted for each side, participants rated hair 1-10
Purpose: To determine whether ACB Kale Protein Blend enhances shine, dry and wet combability, manageability and smoothness of the hair in addition to volume, conditioning, and moisturization
In-vivo Efficacy Data: ACB Kale Protein Blend
Half Head Study:
Half-Head Treated with Positive Control Shampoo and Conditioner
Half-Head Treated with
2.0% ACB Kale Protein
Blend + Base
Shampoo & Conditioner
Figure 1. Full Head Baseline Photo of Untreated Hair Figure 2. Half-Head Treated
In-vivo Efficacy Data: ACB Kale Protein Blend
Half Head Study:
Figure 3. Full Head Baseline Photo of Untreated Hair Figure 4. Half-Head Treated
Half-Head Treated with Positive Control Shampoo and Conditioner
Half-Head Treated with
2.0% ACB Kale Protein
Blend + Base
Shampoo & Conditioner
In-vivo Efficacy Data: ACB Kale Protein Blend
Half Head Study:
Figure 5. Full Head Baseline Photo of Untreated Hair Figure 6. Half-Head Treated
Half-Head Treated with Positive Control Shampoo and Conditioner
Half-Head Treated with
2.0% ACB Kale Protein
Blend + Base
Shampoo & Conditioner
Assessment of Hair Characteristics
0
2
4
6
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12
Ratin
g fr
om 1
(wor
st) t
o 10
(bes
t) Baseline Assessment of Untreated Hair
Assessment of Half Head shampooed withUntreated Control
Assessment of Half Head Shampooed with ACBKale Protein Blend
Assessment of Half Head Conditioned withUntreated Control
Assessment of Half Head Conditioned with ACBKale Protein Blend
Figure 10. Rating of hair characteristics (1-10) following sensory assessment
In-vivo Efficacy Data: ACB Kale Protein Blend
0%
10%
20%
30%
40%
50%
60%
70%
80%
90%Shine
Volume
Dry Combability
Wet Combability
ThicknessSmoothness
Hydration
Softness
Managability
Changes in Hair Characteristics from Baseline to Post Style
In-vivo Efficacy Data: ACB Kale Protein Blend
Percent Difference:Shine: 41%Volume: 47%Dry Combability: 75%Wet Combability: 87% Thickness: 14% Smoothness: 47% Hydration: 30% Softness: 30% Manageability: 63%
0%
10%
20%
30%
40%
50%
60%Shine
Volume
Dry Combability
Wet Combability
ThicknessSmoothness
Hydration
Softness
Managability
Treated Half vs. Control Half After Application & Style
In-vivo Efficacy Data: ACB Kale Protein Blend
Percent Difference:Shine: 37%Volume: 59%Dry Combability: 54%Wet Combability: 50% Smoothness: 32% Hydration: 35% Softness: 55% Manageability: 38%
In-vivo Efficacy Data: ACB Kale Protein Blend
Volumizing Assay
0
2
4
6
8
10
12
14
16
Immediately Following Application 4 Hours After Application
Perc
ent (
%) I
ncre
ase
Effects on Hair Diameter
ACB Kale Protein Blend Wheat Hydrolysate
Protocol
• Equipment: Zeiss AxioplanMicroscope/Ienapol Polarized Light Microscope/iSolution Software
• Materials: 60 individual strands of hair
• Test Quantity: 2.0% in Water
• Frequency of Application: Single Application
• Frequency of Measurement: Baseline, immediately following application & 4 hours after application
In-vivo Efficacy Data: ACB Kale Protein Blend
Hydration Assay
0
2
4
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10
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16
Untreated Control 2.0% ACB Kale Protein Blend 2.0% Wheat Hydrolysate
Perc
ent (
%) I
ncre
ase
Increase in Hair HydrationProtocol• Equipment: DPM 9003 Nova
Impedence Meter
• Principle of measurement: Conductance, single frequency
• Subjects: 10 (m/f)
• Test area: Hair
• Concentration of active used: 2.0%
• Frequency of application: Single Application
In-vivo Efficacy Data: ACB Kale Protein Blend
TEWL Assay
0%
5%
10%
15%
20%
25%
30%
t=24 1 week 2 weeks 3 weeks
Perc
ent (
%) D
ecre
ase
TEWL Comparison Over Time
Experimental vs. Untreated Control Experimental vs. Base Lotion
Protocol
• Equipment: DermaLab Combo
• Principle of measurement: Ultra-sound Probe
• Subjects: 10 (m/f)
• Test area: Volar forearms
• Concentration of active used: 2.0%
• Frequency of application: Twice Daily
In-vivo Efficacy Data: ACB Kale Protein Blend
ORAC Assay
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200
250
200µM Trolox 12.5µM Trolox 0.5% ACB KaleProtein Blend
0.1% ACB KaleProtein Blend
0.05% ACB KaleProtein Blend
Antio
xida
nt C
apac
ity (µ
MTE
)
ORAC Assay
Protocol• Trolox® is used as the positive control
• Solutions were prepared at two concentrations for a reference
• Fluorescent measurements were taken every 2 minutes for 2 hours
• ACB Kale Protein Blend exhibited similar strong antioxidant properties similar to 200µM concentration of Trolox®
In-vivo Efficacy Data: ACB Kale Protein Blend
Cellular Viability Assay
89
105
122
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60
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120
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1.00% ACB Kale ProteinBlend
0.10% ACB Kale ProteinBlend
0.01% ACB Kale ProteinBlend
Viab
ility
(% o
f Con
trol
)
Figure 15. Cellular Metabolism of ACB Kale Protein Blend-treated fibroblasts expressed in terms of percent of control.
Protocol• Human dermal fibroblasts were
seeded into 96-well tissue culture plates
• Concentrations: 1.00%, 0.10%, 0.01%
• Ten microliters of viability reagent was added to 90µL of cell culture media in culture wells
• ACB Kale Protein Blend exhibited comparable results by increasing cell metabolism
ACB Kale Protein Blend
Hair Care Benefits: Increases Softness
Improves Shine
Increases Manageability
Enhances Combability
Increases Volume
Hydrates
Skin Care Benefits: Moisturizes
Conditions
Heals
Improves Barrier function
ACB Kale Protein Blend
Technical Information:
Product Code: 20036
INCI Name: Hydrolyzed Kale Protein & Hydrolyzed Carrot Protein & HydrolyzedLemon Protein
INCI Status: Proposed
Suggested Use Levels: 1.0–10.0%
Suggested Applications: Moisturizing, Film-Forming, Nourishing, Conditioning
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