10 tn International Symposium

ADVANCESAND APPLICATIONS

OF CHROMATOGRAPHYIN INDUSTRY

June 30 - July 4, 1996

held at Faculty of Chemical TechnologySlovak Technical UniversityBratislava, Slovak Republic

ABSTRACTS OF PAPERS

Edited by E. BenickaSlovak Technical University

Faculty of Chemical TechnologyUB/TIB Hannover115 886 32X

89

CONTENTS

LECTURES

Monday, July 1, 1996

GENERAL - PART 1.

Multidimensional Approaches to Trace-Level Analysis by Means of LC and GCU.A.Th.Brinkman 1

Sample Introduction Systems for Improved Trace Analysis by CapillaryGas ChromatographyC.A.Cramers, J.GJ.Mol, J.G.M.Janssen 2

Capillary Zone Electrophoresis of Biopolymers: New Electromigration Mechanismsand Detection TechniquesM.V.Novotny 3

On-Line Use of NMR in Separation Chemistry

KAlbert. G.Schlotterbeck andS.Strohschein 4

THIN LAYER AND COLUMN LIQUID CHROMATOGRAPHY

New Types of Electrochemical Detection for Determination of Carbohydratesand Organic AcidsLJ.Nagels 5Application of Compact Tubes for Preparative Chromatography of ProteinsDj.Josic, A.Strancar, H.Schwinn, M.Barul andA.Podgomik 6

Thin Layer Chromatography Better or Worse than High Performance ColumnLiquid ChromatographyZ.Witkiewicz, MMazurekandZ.Stefaniak 7

Chromatographic Determination of Lipophilicity of Some DrugsH.Kalasz, M.Bathori, S.Hosztafl, T.Csermefy, S.Furst. 8

Optimization of Separation Quality in the Adsorption and Partition LiquidChromatographyT.Kowalska, W.Prus 10

Some Aspects of Adsorption Liquid Chromatography TheoryV.N.Rodionov, B.V.Chemyaev, J.Kfii, Yu.G.Yarkho, A.G.Yurchenko 11

Challenges for the Capillary Optical Fiber Use in Chromatography

J.Doupovec, J.Vykoupil, R.Brunner 13

CAPILLARY ZONE ELECTROPHORESIS

Advances in Non-Aqueous Capillary Electrophoresis and its Applicationin Pharmaceutical an Biomedical AnalysisS.Honori Hansen, I.Bjornsdottir, D.RKepp and J.Tjornelund 15

Continuous Free Flow Electrophoresis - a Powerful Tool for Purification andPreparation of Individual Species Ranging from Small Molecules to CellsG.Weber, P.Bodek 16

Capillary Zone Electrophoresis: An Alternative Technique for Metal TraceAnalysis and SpeciationA.R.Timerbaev 17

Separation Power of Isotachophoresis with Continuous pH and Conductivity GradientsK.Slais 19

Application of HPCE to Analysis and Preparation of Peptide Productsin Pharmaceutical IndustryV.KaSidka, Z.Prusik, J.Hlavddek, I.Svoboda, T.Barth 20

Tuesday, July 2, 1996

SAMPLE PREPARATION AND HYPHENATED TECHNIQUES - PART 1

Evaluation of Techniques for Sample Preparation in Environmental AnalysisF.I.Onuska 22

Analysis of Trace Components in Environmental Samples.Carbonaceous Adsorbents and their Use in Preconcentration Techniquesin Combination with High Resolution Chromatographic SystemsE.Matisova, S.Skrabakovd, MStrakova, M.Marcova 23

Sample Preparation and HPLC in Clinical, Veterinary and Food AnalysisEBrandSteterovd, P.Kubalec 25

Prospects of Hyphenated Methods Towards the Turn of the CenturyP.A.Leclercq ; 27

Gas Chromatography-Mass Spectrometry in Life SciencesH.Gleispach, H.J.Leis, G.Fauler, W.Windischhofer, RKerbl, E.Huber, D.Zach 29

On-line Coupling and Automation in LC/MS and LC/MS/MS Analysisof Environmental SamplesJ.Slobodnik, A.C.Hogenboom, W.M.A.Niessen,B.LM.van Baar and U.A.Th.Brinkman 31

Wednesday, July 3, 1996

CHIRAL SEPARATIONS

Macrocyclic Antibiotics as Chiral Selectors in LC and CED.W.Armstrong 32

Applications of Cyclodextrin-Mediated Capillary Electrophoresis to the Chiral Analysisof Drugs and Related CompoundsJ.Crommen. M.Fillet, I.Bechet, G.Schomburg and Ph.Hubert. 34

VI

Chromatographic Optimization of Selectivity for Direct Capillary Gas ChromatographicMulticomponent Separations of EnantiomersJKrupHik, M.Grefia, l.Spdnik, E.Benickd, J.Hrouzek, I.Skaddni and P.Sandra 35

Determination of Polychlorinated Biphenyl Atropisomers in Environmental SamplesM.J.Gonzalez 37

LC SEPARATION

Prediction and Optimization of Isocratic and Gradient Elution with Binary andTernary Mobile Phases in Normal-Phase HPLCP.Jandera 38

Quantitative Evaluation of Thin Layer ChromatographyM.ProSek. 39

Trace Analysis of Some Organic Compounds by HPLCJ.Lehotay, S.Hatrik 41

Packings with Specific Structural Properties for Chromatographic TechniquesB.Buszewski 43

Chromatographic Bioseparation, Ion-Exchangers of New Types, Theory, Up-Scalingand Industrial ApplicationG.V.Samsonov 44

SAMPLE PREPARATION AND HYPHENATED TECHNIQUES - PART 2

Special Techniques for the Separation of Xenobiotics from the EnvironmentalWater SamplesJ.Tfiska, N.Vrchotova, A.Heyberger -. 45

Use of Distillation as an Environmental Sample Pretreatment Procedure for Analysisof Volatile Organic Compounds by Gas Chromatographywith Special Sample Introduction SystemsB.Zygmunt, W.Janicki 47

Mercury Speciation by HPLC-UV-PCO-CVAAS.A New Analytical Technique and its ApplicationH.F.Schdler, RFalter 48

New Possibilities of GC-MS data Interpretation on the Basis of GC RetentionIncrements of Molecular Structural FragmentsI.G.Zenkevich and A.A.Chupalov 50

Application of Hyphenated Chromatography Techniques for Determinationof Hydrodynamical Parameters of Biological Active MacromoleculesA.K.M.Kabzinski 52

Supercritical Fluid Extraction of Biological Active Compounds from PlantsZ.Suprynowicz. 54

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Development and Creation of Automated Pilot-Plant Scale System to ProduceHigh Pure Isomeric Form of Carotenes from Natural SourcesY.LLukin 55

INDUSTRIAL AND ENVIRONMENTAL APPLICATIONS

High Resolution Gas Chromatography for Process AnalysisJ.Roeraade..... 56

Inverse Gas Chromatography in the Examination of Fractions Separatedfrom Oil Vacuum Distillation ResidueA.Voelkel and J.Fall 58

Ion Chromatography in Environmental Analysis$.Cerjan-Stefanovid 60

Biological Treatment and Chromatographic Determination of Hazardous Compoundsin Evironmental MatricesA.Chmarzynski, K.Gajek, RM.Gadzala andB.Buszewski 62

Applications of Ion Chromatography/. VinS andP.Roztoiil. 63

Determination of Alkylbis(2-benzothiazolyl-sulfen)-amides in the Reactions MixturesUsing Reversed - Phase High Performance Liquid ChromatographyMStolcovd, A.Kaszonyi, M.Hronec 64

PAH - Set and PAH - Mixture - New Slovak Certified Reference Materialsof Polynuclear Aromatic HydrocarbonsI.Culdk, J.Herold, J.CepCek, D.Kordik, L.Majtanovd, A.Kordikovd, Z.Pavlova 66

Thursday, July 4, 1996

GENERAL - PART 2

Towards Higher Speeds of Separation in Column Liquid ChromatographyAn OverviewH.Poppe and J.C. Kraak gg

Polymer Analysis and Characterization by Thermal Field Flow FractionationM.Martin 69

Characterization of Complex Polymer Systems by Exclusion/Adsorption LCCoupled TechniquesD.Berek. 7 0

Some Similarities and Differences between Liquid Chromatography andElectromigration MethodsK.Stulik, V.Pacdkovd 7 2

VM

Capillary Zone Electrophoresis in a Hydrodynamically Closed Separation SystemD.Kaniansky, J.Mardk, M.Masdr, V.Madajovd,E.Simunidovd and V.Zelenskd 73

Unified Retention Concept - Statistical Interpretation of Kovats Retention IndexB.Skrbic 74

Uncertainty Analysis in Chromatography MeasurementsJ.Lawrence and A.D.Lawrence 76

The Influence of Adsorption Phenomena on the Retention of ChromatographedCompounds in Capillary Gas-Liquid ChromatographyV.G.Berezkin 78

POSTERS

SEPARATION MECHANISMS AND RETENTION DATA EVALUATION

A/01 Thermodynamical Characteristics of Sorption and Vaporisation of Alkylbenzenes,Alkylcycloparaffines and AlkyladamantanesA.A.Pimerzin, T.N.Nesterova, l.A.Nesterov. 79

A/02 Identification of C1-C4 Hydrocarbons by Capillary Gas-Solid Chromatographyusing Linear Correlations for Capacity FactorsN.l.Svetlova, V.R.Alishoev, A.l.Allakhverdiev, S.N.Khadzhiev.V.G.Berezkin 80

A/03 Unified Retention Indices of Hydrocarbons on Dinonylphthalate PhaseB.D.Skrbic, J.P.Cvejanov 81

A/04 Use of Principal Component Analysis for the Study of the Influenceof Physicochemical Parameters on the Retention of Barbiturateson Porous Graphitized Carbon ColumnE.Forgacs, T.Cserhdti 82

A/05 The Theoretical Aspects of Non-Equilibrium Chromatographic RegimesG.Elkin 83

A/06 Survey of Numerical Method for Solving Adsorption ChromatographyModelsK.Kaczmarski 84

A/07 Chromatographic Separation of 1,2- and 1,4-chloronitrobenzeneIsomers from Postreaction MixtureW.Zapala, K.Kaczmarski 86

A/08 Displacement Mechanism of Retention in the Reversed Phase TLC Systemswith the Methanol/Water EluentsB.Witkowska-Kita and T.Kowalska 88

A/09 Peak Parameters Estimation using Neural Networks with Back PropagationAlgorithmJ.Hrouzek, S.Hatrik, J.Krupiik 89

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A/10 Using Splines on Moving Grid for Solving Chromatography Column ModelD.Antos, K.Kaczmarski 91

A/11 Correlation Between the Hydrophile-Lipophile Balance (HLB) of Non-IonicSurfactants and their RP-HPLC BehaviorM.Balcan, D.F.Anghel. 93

A/12 Relationship Between the Hydrophobicity and Specific Hydrophobic SurfaceArea of Pesticides Determined by High-Performance Liquid ChromatographyT.Cserhdti, E.Forgdcs 94

A/13 HPLC of a-, (3- Substituted Naphthalenes on Silver-Loaded Stationary PhaseYu.G.Yarkho, V.N.Rodionov, B.V.Chernyaev, A.G.Yurchenko 95

A/14 HPLC of Alkylbiphenyls on Silver Loaded Stationary PhaseV.N.Rodionov, B.V.Chernyaev, J.Kriz. Yu.G.Yarkho, A.G.Yurchenko 97

A/15 Reversed-Phase Retention Characteristics of Some Derivatives of Purine

T.Gondovd, D.burd'dkovd 99

SAMPLE PREPARATION AND HYPHENATED TECHNIQUES

B/01 Preconcentration of BTEX from Water by Means of Solid-Phase MicroextractionM.Strakovd, J.Sedldkovd, E.Matisovd, S.Skrabdkovd, T.Wellsch 100

B/02 Cloud-Point Extraction of Some Pesticides from Environmental Samples andtheir Simultaneous Determination with HPLCG.A.Makharadze, R.LRevia, G.D.Supatashvili 102

B/03 Sorption of Water Soluble Organic Dyes on Magnetic CharcoalM.Safarikovd, I.Safafik 103

B/04 Magnetic Solid Phase Extraction of Planar Organic DyesM.Safarikovd, I.Safafik 105

B/05 Sorbtion of Gibberellins on Microporous Hydrophobic Resins Duringthe Cultivation of Fusarium MoniliformeRRachev, V.Gancheva, S.Bojkova 107

B/06 Extrapolation Models for Supercritical Fluid Extraction of Plastificatorsin Matrices of PolymersK.Ventura, M.Pokorny, J.Vejrosta 108

B/07 The Use of Accelerated Solvent Extraction for Rapid Preparationof Environmental Solid SamplesJ.Gawdzik, M.Mardarowicz, Z.Suprynowicz 110

B/08 Chromatographic Studies on Sorption Kinetics of Selected HazardousCompounds in PlantsA.Gorna-Binkul, B.Buszewski I l l

B/09 Thermal Desorption in Analysis of Natural and Synthetic ProductsLWolska, J.Namiesnik 1 1 2

B/10 A Device for Cryotrapping of Volatile Organic Compounds for theirGas Chromatographic AnalysisW.Janicki. A.Wasik, W.Wardencki, J.Namiesnik 113

B/l 1 Statistical Evaluation and Comparison of Direct Aqueous Injection DAI-GC-ECDand DAI-GC-ECD Combined with Tthin Layer Head Space (THLS)Z.Polkowska, M.Turska, J.Namiesnik 114

B/l 2 Isolation of Polycyclic Aromatic Hydrocarbons from Environmental Samplesby Using Supercritical Fluid ExtractionV.Cizek 115

B/13 Application of High Pressure Soxhlet CO2 Extraction to Preliminary Separationof Organic Environmental PollutantsW.Bakowski, D.Bodzek 116

B/14 Application of Overpressure Thin Layer Chromatography and GC-MS forDetermination of Cholesterol in Consumable Fats of Animal and Vegetal OriginT.Wielkoszynski, B.Jaremczuk, K.Tyrpien, D.Bodzek,B.Janoszka and W.Bakowski 117

B/l 5 Off-line and On-line SPE-HPLC of Itraconazole in Clinical SamplesP.Kubalec, E.BrandSteterovd 118

B/l 6 Determination of Solanidine in Human Plasma by Gas Chromatography- Mass SpectrometryA.Trendansky 120

B/l 7 HRGC/LRMS Analysis of Planar Polychlorinated Biphenylsin Human Fat SamplesJ.Chovancovd, B.Drobnd, A.Koian, J.Petrik, H.Uhrinovd 121

B/l 8 Rapid Determination of PAH in Oil Matrix Using HPLC and GC-MS AnalysisA.Kot, M.Slebioda, A.M.Kolodziejczyk 123

B/l 9 Analysis of Organic Mercury in the Environment by Using SFE-GC-AED SystemI.Ostrovsky, RKubinec, RMrainovd andL.Sojdk 124

B/20 GC-FTIR Separation and Identification of Mononitro and Dinitro Isomersfrom Nitration Mixtures of Methyl ArylacetatesL.Sojdk, A.Perjessy, RKubinec, I.Ostrovsky andA.G.Giumanini 125

B/21 CGC-MSD of Lower Oxyethylenated Aliphatic Alcohols after their Conversioninto TMS DerivativesK.Komdrek, V.Pitthard, E.Kostrubaniiovd, S.Skvarenina 127

B/22 Identification of Selected Classes of Organic Pollutants in Marine Sedimentsof Harbour Basins by Gas Chromatography - Mass Spectrometry andHigh Performance Liquid ChromatographyB.Makuch, K.Olenczuk-Neyman andB.Zygmunt 129

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B/23 GC and GC/MS Identification and Determination of Ethylene and PropyleneGlycol - Based Coalescing AgentsZ.DuSek 130

B/24 Identification of Structure Units Constituting Kerogen by Pyrolysis,GC and GC/MSS.Svel-Ceroveiki, I.ESkinja, A.Alajbeg 131

B/25 Key Volatile Flavour Compounds of Pressure-Cooked Hen MeatP.FarkaS, J.Sddeckd, M.Kovdd, J.Pefka.B.Siegmund, E.Leitner, WPfannhauser 132

GAS CHROMATOGRAPHY

C/01 Certified Reference Standards - N-alkanesSMusil, LVyskoftlovd, V.Csorgoovd 133

C/02 Determination of Normal Boiling Points of Cyclohexane and AdamantaneDerivatives by Means of GLCKLKrasnykh, V.S.Sarkisova, V.E.Zuzin, A.A.Pimerzin 134

C/03 Determination of Volatile Organic Compounds Using Gas Chromatography andPurge and Trap InjectionA.Gierak 135

C/04 Generation of Standard Gaseous Mixtures by Thermal Decompositionof Surface CompoundsP.Konieczka, M.Prokopowicz, A.Skwierawska, J.Namiesnik, J.F.Biernat 136

C/05 Comparison of Various Kinds of GC Analysis of Ethylene Oxide in PolymerMedical DevicesI.Buben, P.Holer 138

C/06 Research Test of SFg in Work Areas of Electrotechnical Equipments Filledwith SFg.H.Slowikowska, I. Losiewicz 139

C/07 Methods of Accuracy Control in Determination of Impurity Traces in VolatileSubstancesV.A.Krylov 140

C/08 Gas Chromatography Analysis of Methanol Oxidation ProductsS.Krvstev, K.Ivanov. 141

C/09 Determination in Water and in the Air of Same Substances for PiretroidPesticides Synthesis/. Halkevich, O.Manenko, D. Rogovski, N. Feduschak, Y. Bidnichenko 143

C/10 Gas Chromatography Method of Determination of Uracil Herbicides Residuesin Medicinal PlantsJ.Tekei; SJahomd, andS.Vaverkova. 144

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C/l 1 Determination of Formic Acid Using Reaction Gas ChromatographyS.Hladiy, P.Pasichnyk, M.Starchevsky, Yu.Pazdersky 145

C/12 The Chromatographic Methods in the Production of Pure Hydroxyl Amine SaltsR.Gireth. M.Krdlik 146

C/l 3 GLC and HPLC Analysis of Condensates Arising in the Processof Beech Wood SteamingF.Kacik, D.Baloghovd, J.Budko 148

C/14 Application of the Chelate-Containing Stationary Phase for GC Determinationof Aniline and its DerivativesY.Slizhov, S. Budkova 149

C/15 Determination of cis- and /rara-isomeric of Unsaturated Fatty Acids by GasChromatographic MethodY.M.Procyk, l.F.Rivis, B.B.Danylic 150

C/l 6 Assay of the Results of Gas-Chromatographic TestsB.B.Danylic, l.F.Rivis. Y.M.Procyk. 151

C/17 Simultaneous Determination of Common, Esterified and Unestenfied Fatty Acidsl.F.Rivis. B.B.Danylic, Y.M.Procyk. 152

C/l 8 The Use of Capillary GC for Determining the Hydrogenation Level of Edible OilsM.Naglid, A.Smidovnik 154

C/19 Utilisation of Chromatographic Methods in the Field of Edible Fatand Oil TechnologyV.Koman 155

THIN LAYER CHROMATOGRAPHY

D/01 The Chromatographic Detection of Ecdysteroids in PlantsL.N.Zibareva 157

D/02 Determination of Acyl Conversion in Vegetable Oil Methyl EstersZ.CvengroSovd, J.CvengroS, M.Hronec 158

D/03 Determination of Oxidative Degradation Products of EthylenebisditiocarbamateFungicides Zineb and Nabam with Potassium Permanganateby Thin Layer ChromatographyP. Vassileva-Alexandrova, A.Neytcheva, K.Ivanov, M.Nikolova 160

D/04 N-alkyl Bonded Volcanic Tuff - New Stationary Phases for RP-TLCM.-V. Coman, C.Marutoiu 162

D/05 Quantitative Evaluation of Thin-Layer Chromatograms from TotalyIlluminated TLC platesl.Vovk, M.ProSek 163

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D/06 Use of Chromatography in Thin Layer Sorbent for Determination of Diprophenin Biological MaterialM.M.Orlynski 164

D/07 Quantitative Chromatographic Analysis by Color Analyzer Datacolor 3890

M.Petrovid, Lj.Bokid, S.AndraSid, M.KaStelan-Macan 165

LIQUID CHROMATOGRAPHY

E/01 HPLC Analysis of Lipophile Vitamins in Vegetable OilsZSalkovd, A.taiky 167

E/02 Preparative Chromatography as Operation in Isolation of Diolon Acetatewith Pharmaceutical PurityW.Piatkowski, D.Antos, W.Mazurkiewicz, K.Kaczmarski 169

E/03 Application of Column Preparative Chromatography to the Separationof Hydroxycholanic Acids from Animal BileW.Piatkowski, K.Kaczmarski, D.Antos, W.Zapala 171

E/04 Sorption Technology of Protein Medicines Including of Non-EquilibriumChromatographic EffectsN.Glazova 173

E/05 Chromatographic Purification of Enzyme MedicinesN.V.Zainkova, L.M.Igonina, N.V.Rudometova, G.E.Elkin, N.V.Glazova 175

E/06 The Phenomenological Approach to Optimization of Industrial ChromatographicProcesses for Producing of MedicinesN.Rudometova, L.Kurdyavka, N.Glazova, G.Elkin 176

E/07 Application of a New Chromatographic Method - LC PEAT in the Characterizationof Polystyrene-graft-Poly(ethylene oxide) CopolymersR.MurgaSovd, D.Berek, I.CapekandE.Lathovd 177

E/08 Characterisation of Poly(2,6-dimethyl-1,4-phenylene oxide) by GPCand ViscometryM.Cholinska, Z.Dobkowski 179

E/09 Full Adsorption/Desorption - SEC Coupling as a Powerful Toolin Polymer AnalysisS.H.Nguyen, M.JanCo and D.Berek 180

E/l 0 Separation of Block Copolymers from Parent Homopolymers by Using LiquidChromatography at the Point of Exclusion - Adsorption TransitionM.JanCo, D.Berek, W.Schnabel, Ch.-H.Fischer, Y.Yagci andA.Onen 181

E/l 1 Enzyme Thermistor as a Detector in Liquid ChromatographyP.Dodolomansky, A.Breier, P.Gemeiner 183

E/l 2 Conductive Polymer Blends for use as Electrode Material in HPLC AmperometricDetectionKStaes, L.J.Nagels 184

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E/13 The Enhanced Use of HPLC-Hardware and Software for Express Analysisof Unknown MixturesM.Kossenko, V.Tkachenko, D.Sanagurskyj, l.Kotsumbas 185

SORBENTS

F/01 Characterization and Evaluation of Mercaptopropylmethyl PolysiloxaneStationary Phases for Capillary Complexation Gas ChromatographyW.Wasiak. RWawrzyniak. 186

F/02 Thermodynamic Characteristics of Adsorption of Organic Compoundson Silica Gels with Bonded Amino and Thiol GroupsK.B.Gurevich, T.M.Roschina, V.Ya.Davydov, A.A.Mandrugin 187

F/03 Ion-Exchange Properties of Glutamic Acid-Bonded SilicaA.I.Elefterov, M.G.Kolpachnikova, P.N.Nesterenko, O.A.Shpigun 188

F/04 Analysis of Structure of PE Copolymers by TREF/SECP.Hudec 189

F/05 Radiation -Chemical Modification of Polymer Sorbent for Gas ChromatographyP.V.Zibarev 190

F/06 New Polymer Sorbents for Gas Chromatography Nitrated Under the Actionof X-Ray IrradiationT.S.Shepelenko, P.V.Zibarev. 191

F/07 Application of Modified Polymer Sorbents for ImmunosorptionE.V.Belousov, P.V.Zibarev, E.Ts.Dambaev, A.A.Kukoviakin 192

F/08 Phenomena of Synergism and Competition During MulticomponentProtein SorptionA.A.Demin, A.T.Melenevsky, E.B.Chizhova, G.V.Samsonov 193

F/09 Application of the New Supports for High Performance Covalent AffinityChromatography for Separation of Thiolproteins and MetallothiolproteinsA.K.M.Kabzinski 194

F/10 New Adsorbents Prepared by Modification of PTFELFeltl, P.Cervenkova andJ.Hlavaty 195

F/l 1 New Application of Microcrystalline Cellulose in the Thin Layer ChromatographyN.E.Kotelnikova 196

F/l 2 Interactions of Nucleophilic Adsorbates of Charge-Transfer Typewith Cu(II) Complexes Chemically Bonded with SiO2 byDiphenylphosfine and p-diketonate GroupsW.Wasiak, I.Rykowska 197

F/l 3 Physical Characterization and MAS-NMR Spectrometry of Carbon SorbentsUsed for Preconcentration PurposesS.Skrabdkovd, E.Matisovd, I.Novdk, RBrindle, K.Albert, J.Kozdnkovd 199

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F/I4 Study on Macroporous Carbon Sorbents by Gas-Solid ChromatographicDetermination of Specific Retention VolumesS.Skrabdkovd, M.Slezadkovd, E.Matisovd 201

F/15 Separation of Polycyclic Aromatic Hydrocarbons by Capillary GasChromatography Using High Temperature Liquid CrystallineStationary PhasesA.Kraus, G.Kraus. RKubinec, I.Ostrovsky, L.Sojdk 203

F/16 Chromatographic Separation of [l,3]oxazepino[3,2-a]indole Isomersby Means of Cellulose-Based AdsorbentsA.MaruSka, E.Machtejevas, V.Martynaitis, A.Sadkus 205

ENVIRONMENTAL APPLICATIONS

G/02 Peculiarities of Hydrocarbon Distribution Over Oil and Gas FieldsILitvin, J.Hansen 207

G/03 Some Results of Complex Thermogasochemical and Microbiological MethodApplication in Pre-surface Deposits in South-East of TatariaILitvin, T.Wrublevska 208

G/04 GC Analysis of Petroleum Hydrocarbons Contamination in Soil SamplesRKubinec, L.Sojdk, I.Ostrovsky, RMra&novd,A.Kraus and A.Eszenyiovd 209

G/05 Polycyclic Aromatic Hydrocarbons in Air and Soil of North Croatial.ESkinja, M.Eikinja, andZ.Soljii 211

G/06 Application of SPME for Determination of Organic Pollutant in WorkplaceAtmosphereD.Gorlo, LWolska, J.Namiesnik 213

G/07 Determination of Selected Volatile Organic Compounds in Working PlacesAtmosphereB.Kozdron-Zabiegala, Jacek Namiesnik 214

G/08 Determination of Some Toxic Organic Compounds in Waste Gas by HPLCKHromuldkovd, J.Lehotay 215

G/09 Frontal Concentration of the Toxic Derivatives of Arsenic, Tellurium andSelenium from the AirZ.P.Vetrova, LA.Ivanovo, N.T.Karabanov 216

G/10 Application of Gas Extraction Technique (P&T) for the Gas ChromatographicDetermination of Volatile Sulphur Compounds in Different WatersW.Wardencki, W.Janicki, J.Namiesnik 217

G/l 1 The Testing of Silicone-Tubing-Probe Measuring System for the Monitoringof Volatile Pollutants in Water SamplesM.Strakovd, E.Matisovd, A.Ferancovd, S.Skrabdkovd, D.BOttger 218

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G/12 Application of Solid Phase Microextraction for Determination of VolatileOrganohalogen Compounds in Water SamplesJ.Czerwinski, J.Namiesnik 220

G/13 Application of Thin Layer Head Space With Self Generation of Liquid Sorbentfor the Determination of Volatile Organic Halogen Compounds in Waste WaterZ.Polkowska, M.Turska. J.Namiesnik 221

G/14 Trace Analysis of Triazine Herbicides in Biological MaterialsM.Veningerovd, M.Lukdcsova, Cs.MiSl'anovd 222

G/15 Analysis of Polychlorinated Biphenyls in Bacterial Cell SuspensionsB.Vrana, S.Baldz, K.Dercovd 223

G/16 Congener-Specific Analysis of Polychlorinated Biphenyl MixturesDuring BiodegradationK.Dercovd, S.Baldz, B.Vrana 225

G/17 Micro-Extraction by Demixing for the Determination of Phenolsfrom Water SamplesF.I.Onuska, K.A.Terry 227

G/18 Large Volume Injections in HPLC and Packed Column SFCA.Kot, P.Sandra, A.M.Kolodziejczyk 228

G/l 9 Optimization of the Procedure of Preconcentration and Elution of SelectedPesticides Using SPE Cartridges and Extraction DisksM. Biziuk, J. Czerwinski, M. Wiergowski, I. Strzelecka, H. Wachowska 229

G/20 Determination of Selected Pesticides in the Sea and Estuarine Waters AfterSorption on XAD-2 Solid SorbentM.Biziuk, J.Tronczynski 230

G/21 Accumulation and Toxicity of Bimetallic Combinations of Cd2 + , Zn 2 + , Cu 2 +

and Fe2"1" in Scenedesmus Quadricauda AlgaA.Fargaiovd. 231

HPLC APPLICATIONS

H/01 Size Exclusion Chromatography of Condensates of Naphthalenesulfonic Acidswith FormaldehydeA.A.Kapura 233

H/02 Comparative Liquid Chromatographic Analysis of Composition of FlameRetardants for Fabric Containing N-methylol-3-dimethoxyphosphorylpropionamideA.A.Kapura 234

H/03 Single-column Ion Chromatography of Linear PolyphosphatesLSvoboda, U.Schmidt 236

H/04 Metals Removal by Ion-Exchange From Plating RinsewaterSCerjan-Stefanovie, D.GrubiSa 238

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H/05 The Use of Ion-Interaction Chromatography with Chelating Agentsin the Electroplating IndustryE.Naujalis, A.Judzentiene, A.Padarauskas 240

H/06 Temperature Effect During Isocratic Micro-HPLC Separation of PolycyclicAromatic Hydrocarbons (PAHs)RM.Gadzala, B.Buszewski 241

H/07 Chromatography of Barley (3-GlucansJ.Copikovd, A.Sinica, K.Vaculovd 242

H/08 Improved Method for the HPLC Determination of Amino Acids withDiethylethoxymethylene MalonateM.Hudec, A.Surovcovd 243

H/09 Study of Chromatographic Behaviour of Barbiturates in Micellar LiquidChromatography with Sodium Cholate and Sodium DodecylsulfateVMOkujava, M.Rukhadze. M.V.Sebiskveradze 245

H/10 Optimization of Separation Conditions for Industrial Analysis of Vitaminsby High Performance Liquid Chromatography MethodV.N.Filimonov, L.N.Balyatinskaya, l.F.Kolosova,L.V.Denisova, N.I.Avtsinova 246

H/l 1 Reverse-Phase HPLC of Fat-Soluble Vitamins in Multivitamin FormulationsL.V.Denissova, L.N.Balyatinskaya, V.N.Filimonov, I.F.Kolossova 247

H/l 2 Determination and Pharmacokinetics of Frusemide and Amiloride afterIngestion of Complex PreparationA.Skorek-Jankowska, A.Jankowski, H.Lamparczyk 248

H/l 3 Determination of Oxacillin, Cloxacillin and Dicloxacillin in Food SamplesP.Kubalec, E.BradSteterovd, A.Bedndrikovd, E.Valentovd, L.Machdikovd 249

H/l 4 Achiral and Chiral HPLC of Some Antiarrhythmics and Their MetabolitesD.Krdlikovd, E.BrandSteterovd, L.Bozekovd 251

H/l 5 HPLC - Determination of Total Hydroxyproline in UrineMethod and ImportanceK.Rdcovd, l.Blandrik, S.Podand 253

H/l 6 Preparative Immobilized Metal Affinity Chromatographyof Human Growth HormoneJ.Liesiene, KRaCaityte, M.Morkeviiiene, P.Valandius, V.Bumelis 254

H/17 Chromatography of 1-deoxy-l-nitroalditols on a Cation-Exchange Resinin the Lanthanum FormLPetruS, M.PetruSovd, E.Lattovd 255

H/l 8 Determination of Primary Oxidation Products of Linoleic Acid by HPLCN.GladoviC, LZupandii-Kralj, J.Plavec 256

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H/l9 A Column for Rapid High-Resolution Liquid ChromatographicAnalysis of AcyclovirA.Peiavar, M.ProSek 257

H/20 Examination of In Vitro Equivalence of Extended Release TabletsJ.Simon, I.Toth, E.Bdrczay, M.Gora 258

ENANTIOSELECTIVE SEPARATION

I/O 1 Evaluation of Enantioselective Interactions at Direct Capillary GasChromatographic Separations of EnantiomersI.Spdnik, J.KrupCik, E.Benickd, F.l.Onuika, P.Sandra 259

1/02 On the Rotational Bariers of Atropisomers of Some PCBs and their GasChromatographic SeparationsM.Mdjekovd, P.Mdjek, E.Benickd, J.Krupdik, F.OnuSka, P.Sandra 261

1/03 Gas Chromatographic Separation of PCB Enantiomers on CyclodextrinStationary PhasesE.Benickd, D.TakdCovd, J.Krupdik, I.SkaCdni, F.Onuska 263

1/04 The Study of the Separations Mechanisms in Chiral GC by Chemometric ToolsT.Hevesi, J.R.Chretien, E.Benickd, J.KrupCik 264

1/05 Examples of Enantioselective Separation on Cyclodextrin Stationary PhasesE.Benickd, I.Spdnik, J.Krupiik and P.Sandra 265

1/06 Easy and Efficient Way of Preparing Supports for High Performance LiquidChromatographyM.Bdrtlovd, M.Capka, M.Popl, M.Czakoovd 266

1/07 RP-HPLC Methods for the Resolution of the Enantiomers of P-Amino AcidDerivatives of Cycloalkane SkeletonG.Tordk, A.Peter, M.Peter, P.Csomos, F.Fuldp, GBerndth 268

1/08 Enantioseparation of Flobufen Using Different Separation Systems of HPLCand MEKCE.Tesafovd, M.Gilar, A.Jegorov, M.Uhrovd, Z.Deyl 269

ELECTROMIGRATION METHODS

J/01 Investigation of Triticale by the Methods of ElectromigrationH.Spiridovich, A.Veevnich, L.Goncharova 270

J/02 Determination of Piracetam in Human Plasma by Capillary ElectrophoresisP.Kowalski, D.Rajzer, H.Lamparczyk 271

J/03 Determination of Coumarins from Artemisia abrotanum L. andIntractum abrotani by Capillary ElectrophoresisRJ.Ochocka, Z.Thiel, H.Lamparczyk 272

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J/04 Study of Separation of Cypermethrine Hydrolysis Degradation Productsby MECCRMachaC J.Sevdik, VDostdl, Z.Strdnsky, K.Lemr 274

J/05 The Study of Cypermethrine Stereoisomer SeparationJ.Sevdik, K.Lemr, D.Jirovsky. Z.Strdnsky, T.Veiefa 275

J/06 The use of Capillary Electrophoresis for the Study of Glycosylation of PeptidesJ.Jiskra, VPacdkovd, K.Stulik 276

J/07 Performance of Focusing of Ampholytes by Displacement Electrophoresiswith Continuous pH Gradient Moving in Tapered ChannelM.St'astnd, K.Slais 277

J/08 Determination of Two Metoprolol Metabolites in Human Urine by CapillaryIsotachophoresisJ.Sddeckd, J.Polonsky, H.Shintani 278

J/09 Determination of Preservatives in Food Products by ITPJ.Karoviiovd, P.Simko, J.Polonsky, A. Rajniakovd 280

J/10 Characterisation of Wheat Gluten by SE- and RP- HPLC Methods andComparison with SDS-PAGEAGolc-Wondra, M.ProSek 282

J/l 1 Determination of Mexiletine by Capillary IsotachophoresisT.Buzinkaiovd, J.Polonsky, I.Skaedni 283

J/12 Determination of Mushroom Toxins by Gel ElectrophoresisY.I.Bidnichenko 285

J/13 Capillary Zone Electrophoresis of NitrophenolsV.Madajovd, E.KrCmovd, D.Kaniansky, M.Masdr, J.Mardk and F.I. OnuSka 286

J/14 Determination of Inorganic Anions by Capillary Zone Electrophoresis withConductivity DetectionD.Kaniansky, V.Zelenskd, D.Baluchovd 287

LAST MINUTE POSTERS

K/01 The Possibility of Analytical Control over the Obtaining Procedeumof IndomethacinD.Rusu, C.Cimpoiu, T.Hodisan 288

K/02 Liquid Chromatographic Behavior of Mercury(II), Methylmercuryand PhenylmercuryM.Hutta, M.Foltin, S.Megovd, S.Rippa, T.Prochdckovd,J.Kandrdt, M.Stekldd, A.Kianic'kovd, M.Chaldnyovd 290

K/03 HPLC Determination ofTylosin in MilkJ.Sokol, EMatisovd, E.Dudrikovd, RCabadaj 291

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K/04 Characterization of Chemical Heterogeneity of a Graft Copolymerby Conventional SECL.Mrkvidkovd 292

K/05 Compensation of Instrument Parameters in Size Exclusion Chromatographywith Dual DetectionM.NetopilikB.Porsch 293

K/06 Isolation and Identification of Heavy Metal Binding Proteins from Green Alga,Chlorella, Using Size Excludion Liquid Chromatography with UV Photometric,AAS and ICP-MS DetectionH. Vodickovd, P.Mader, I. Stejskalovd, W. Gossler, V. Pacdkovd, K. Stulik 294

K/07 Capillary Electrophoresis of CytokininsV.Pacdkovd, K.Stulik, V.Vlasdkovd 295

K/08 Capillary Electro-Chromatography : Normal Phase ModeAMaruika, U.Pyell .' 296

K/10 Gel Permeation Chromatography in Studies of Mechanical Pre-Treatment Effecton Cellulose BiodegradationG.Strobin, D.Ciechanska, D.Wawro, S.BoryniecandH.Struszczyk 297

K/l 1 Chromatographic Peak Distortion PhenomenonS.Hladyi, M.Starchevsky, Yu.Pazdersky 298

Author Index 300

XXI