Structural elucidation, antioxidant and immunomodulatory ...
ABSTRACT It is well known that the natural plants extracts are beneficial for humans thanks to...
1
ABSTRACT It is well known that the natural plants extracts are beneficial for humans thanks to their antioxidant, anti-inflammatory and immunomodulatory effects. The biological activity of these berries is mainly due to their high content of anthocyanins and other polyphenols. The nanoparticles facilitate the penetration of substances in skin, enhancing their antimitotic, anti-inflammatory and antibiotic properties. We have investigated the pH influence on the morphology of some new hybrid materials based on gold nanoparticles and natural extracts from fruits of Romanian native plants from Adoxaceae family (Viburnum opulus L. and Sambucus nigra L.). We have chosen the optimal method to get these materials in which gold nanoparticles of 10-80 nm were obtained. We characterized them by UV-Vis and FT-IR spectroscopy, by TEM and DSC. The hybrid materials have been tested on psoriatic lesions, but only after their non toxicity were determined by means of in vitro and in vivo analysis. The medical results emphasized a remarkable improvement of these lesions. Effect of Natural Extracts pH on Morphological Characteristics of Hybrid Materials Based on Gold Nanoparticles Liliana Olenic 1 , Adriana Vulcu 1 , Ioana Chiorean 2 , Maria Crisan 3 , Camelia Berghian Grosan 1 , Simina Dreve 1 , Luminita David 4 , Lucian Barbu-Tudoran 5 , Irina Kacso 1 , Ioan Bratu 1 , Camelia Neamtu 1 and Cezara Voica 1 1 National Institute for Research and Development of Isotopic and Molecular Technologies, 65-103 Donath Street, Cluj-Napoca, Romania 2 Faculty of Mathematics and Informatics, Babeş-Bolyai –University, Kogălniceanu Street, no.1, Cluj-Napoca, Romania 400084 3 Histology Department “Iuliu Haţieganu” University of Medicine and Pharmacy, Emil Isaac Street no.13, Cluj-Napoca, Romania 4 Univ Babes Bolyai, Fac Chem & Chem Engn, RO-400028 Cluj- Napoca, Romania 5 Faculty of Biology and Geology Babes-Bolyai University, Bilascu Street no.44, Cluj-Napoca Romania EXPERIMENTAL CONCLUSIONS: Gold nanoparticles functionalized with anthocyanins extracted from Viburnum opulus L. and Sambucus nigra L. (AuNPs-C and AuNPs-S) were prepared at different pH (4-11) and were characterized by UV-Vis and FTIR spectroscopy and by TEM (at pH 7-9:diameters between 10-80 nm); There were assessed cytotoxicity tests (of the extract alone and of the functionalized nanoparticles) and the antiinflammatory tests (on keratinocytes treated with non-toxic doses of nanoparticles exposed before to increasing doses of UVB irradiation) for hybrid materials obtained at pH 7 respectively 9.5; The histograms of the skin thickness for 8 patients presented an Acknowledgement This work was elaborated in the frame project no 147/2012, through the program “Partnerships in priority areas-PN II”, developed with the support of ANCS, CNDI- UEFISCDI HO OH O OH OH pH acid O sugar HO OH O OH O O sugar pH neutru HO OH O O - O O sugar pH bazic FT-IR spectroscopy X-ray Diffraction RESULTS and DISSCUTIONS: Fig. 1 Formulas of majoritar compounds anthocyanins- from natural extracts Fig. 2 TEM images of: AuNPs-C, AuNPs-S and UV-Vis spectra of : AuNPs-C; AuNPs-S 300 400 500 600 700 800 900 0.0 0.2 0.4 0.6 0.8 1.0 1.2 1.4 1.6 A uN P s-E 2 pH 11 A uN P s-E 2 pH 9.5 A uN P s-E 1 pH 7.3 A b so rb an ce (a.u .) nm ) A uN P s-E 1 pH 11 0 20 40 60 80 100 0 20 40 60 80 100 120 140 160 180 200 I (a .u .) 2 A uN P s-E 1 111 200 220 311 222 0 20 40 60 80 100 0 50 100 150 200 I(a.u .) 2 A uN P s-E 2 111 200 220 311 222 Fig. 3 X-ray spectra of nanomaterials Fig. 4 FT-IR spectra of nanomaterials at different pH Fig. 5 Histograms of the skin thickness (in μm) : AuNPs-C, AuNPs-S, Nanomaterials preparation and characterization Skin before and after treatm 300 400 500 600 700 800 900 0.10 0.15 0.20 0.25 0.30 0.35 0.40 0.45 0.50 0.55 0.60 0.65 0.70 0.75 A uN P S -E 1pH 7 .3 cen trif A uN P S -E 2pH 9.5 cen trif A b so rb an ce (a .u .) (nm ) Scheme 1 Reaction scheme for obtaining gold nanoparticles HAuCl 4 + O O O O R R R: HO OH O OH OH O sugar HO OH O O sugar 500 1000 1500 2000 2500 3000 3500 4000 4500 0 1 2 3 4 5 6 7 A uN P s-C pH 4 C pH11 A uN P s-C pH 7 A b so rb an c e (a.u .) (nm ) A uN P s-C pH 11 C pH 7 1398 1633 1407 1588 1734 2930 2921 3401 3419 1072 1026 500 1000 1500 2000 2500 3000 3500 4000 4500 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 S pH 11 S pH 9 A uN P s-S pH 11 A b so rb an ce (a.u .) (nm ) A uN P s-S pH 9 1389 1588 1081 1398 1633 2921 3428 3392 3383 3428 2921 2938 2930 1072 1081 1633 1452
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Transcript of ABSTRACT It is well known that the natural plants extracts are beneficial for humans thanks to...
ABSTRACT It is well known that the natural plants extracts are beneficial for humans thanks to their antioxidant, anti-inflammatory and immunomodulatory effects. The biological activity of these
berries is mainly due to their high content of anthocyanins and other polyphenols. The nanoparticles facilitate the penetration of substances in skin, enhancing their antimitotic, anti-inflammatory and antibiotic properties.
We have investigated the pH influence on the morphology of some new hybrid materials based on gold nanoparticles and natural extracts from fruits of Romanian native plants from Adoxaceae family (Viburnum opulus L. and Sambucus nigra L.). We have chosen the optimal method to get these materials in which gold nanoparticles of 10-80 nm were obtained.
We characterized them by UV-Vis and FT-IR spectroscopy, by TEM and DSC. The hybrid materials have been tested on psoriatic lesions, but only after their non toxicity were determined by means of in vitro and in vivo analysis. The medical results emphasized a remarkable improvement of these lesions.
Effect of Natural Extracts pH on Morphological Characteristics of Hybrid Materials Based on Gold Nanoparticles
Liliana Olenic1, Adriana Vulcu1, Ioana Chiorean2, Maria Crisan3, Camelia Berghian Grosan1, Simina Dreve1, Luminita David4, Lucian Barbu-Tudoran5, Irina Kacso1, Ioan Bratu1,
Camelia Neamtu1 and Cezara Voica1 1National Institute for Research and Development of Isotopic and Molecular Technologies, 65-103 Donath Street, Cluj-Napoca, Romania
2Faculty of Mathematics and Informatics, Babeş-Bolyai –University, Kogălniceanu Street, no.1, Cluj-Napoca, Romania 4000843Histology Department “Iuliu Haţieganu” University of Medicine and Pharmacy, Emil Isaac Street no.13, Cluj-Napoca, Romania
4 Univ Babes Bolyai, Fac Chem & Chem Engn, RO-400028 Cluj- Napoca, Romania5Faculty of Biology and Geology Babes-Bolyai University, Bilascu Street no.44, Cluj-Napoca Romania
EXPERIMENTAL
CONCLUSIONS:Gold nanoparticles functionalized with anthocyanins extracted from Viburnum opulus L. and Sambucus nigra L. (AuNPs-C and AuNPs-S) were prepared at different pH (4-11) and were characterized by UV-Vis and FTIR spectroscopy and by TEM (at pH 7-9:diameters between 10-80 nm); There were assessed cytotoxicity tests (of the extract alone and of the functionalized nanoparticles) and the antiinflammatory tests (on keratinocytes treated with non-toxic doses of nanoparticles exposed before to increasing doses of UVB irradiation) for hybrid materials obtained at pH 7 respectively 9.5; The histograms of the skin thickness for 8 patients presented an involution of diseases after treatment, fact that indicates the very good antiinflammatory effect of our nanomaterials. Processes in Isotopes and Molecules (PIM 2013), September 25-27, 2013, Cluj-Napoca, ROMANIA
AcknowledgementThis work was elaborated in the frame project no 147/2012, through the program “Partnerships in priority areas-PN II”, developed with the support of ANCS, CNDI-UEFISCDI
HO
OH
O
OH
OH
pH acid
Osugar
HO
OH
O
OH
O
Osugar
pH neutru
HO
OH
O
O-
O
Osugar
pH bazic
FT-IR spectroscopy X-ray Diffraction
RESULTS and DISSCUTIONS:
Fig. 1 Formulas of majoritar compounds anthocyanins- from
natural extracts
Fig. 2 TEM images of: AuNPs-C, AuNPs-S and UV-Vis spectra of : AuNPs-C; AuNPs-S
300 400 500 600 700 800 900
0.0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
AuNPs-E2 pH 11
AuNPs-E2 pH 9.5
AuNPs-E1 pH 7.3
Ab
sorb
ance
(a.
u.)
nm)
AuNPs-E1 pH 11
0 20 40 60 80 1000
20
40
60
80
100
120
140
160
180
200
I (a
.u.)
2
AuNPs-E1111
200
220 311222
0 20 40 60 80 1000
50
100
150
200
I (a
.u.)
2
AuNPs-E2
111
200220 311
222
Fig. 3 X-ray spectra of nanomaterials
Fig. 4 FT-IR spectra of nanomaterials at different pH
Fig. 5 Histograms of the skin thickness (in μm) : AuNPs-C, AuNPs-S,
Nanomaterials preparation and characterization
Skin before and after treatment
300 400 500 600 700 800 900
0.10
0.15
0.20
0.25
0.30
0.35
0.40
0.45
0.50
0.55
0.60
0.65
0.70
0.75
AuNPS-E1pH 7.3 centrif
AuNPS-E2pH 9.5 centrif
Ab
sorb
ance
(a.
u.)
(nm)
Scheme 1 Reaction scheme for obtaining gold nanoparticles
HAuCl4 +
O
O
O
O
R
R
R:
HO
OH
O
OH
OH
Osugar
HO
OH
O
Osugar
500 1000 1500 2000 2500 3000 3500 4000 45000
1
2
3
4
5
6
7
AuNPs-C pH 4
C pH11
AuNPs-C pH 7
Ab
sorb
ance
(a.
u.)
(nm)
AuNPs-C pH 11
C pH 7
13981633
1407 1588
17342930
2921
3401
3419
1072
1026
500 1000 1500 2000 2500 3000 3500 4000 4500
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
S pH 11
S pH 9
AuNPs-S pH 11
Ab
sorb
ance
(a.
u.)
(nm)
AuNPs-S pH 91389 1588
1081 1398 1633 29213428
3392
3383
34282921
29382930
1072
1081 16331452
