About OMICS Group
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Transcript of About OMICS Group
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About OMICS Group OMICS Group International is an amalgamation of Open Access publications
and worldwide international science conferences and events. Established in the year 2007 with the sole aim of making the information on Sciences and technology ‘Open Access’, OMICS Group publishes 400 online open access scholarly journals in all aspects of Science, Engineering, Management and Technology journals. OMICS Group has been instrumental in taking the knowledge on Science & technology to the doorsteps of ordinary men and women. Research Scholars, Students, Libraries, Educational Institutions, Research centers and the industry are main stakeholders that benefitted greatly from this knowledge dissemination. OMICS Group also organizes 300 International conferences annually across the globe, where knowledge transfer takes place through debates, round table discussions, poster presentations, workshops, symposia and exhibitions.
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About OMICS Group Conferences
OMICS Group International is a pioneer and leading science event organizer, which publishes around 400 open access journals and conducts over 300 Medical, Clinical, Engineering, Life Sciences, Pharma scientific conferences all over the globe annually with the support of more than 1000 scientific associations and 30,000 editorial board members and 3.5 million followers to its credit.
OMICS Group has organized 500 conferences, workshops and national symposiums across the major cities including San Francisco, Las Vegas, San Antonio, Omaha, Orlando, Raleigh, Santa Clara, Chicago, Philadelphia, Baltimore, United Kingdom, Valencia, Dubai, Beijing, Hyderabad, Bengaluru and Mumbai.
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A Metabolomics Approach to Study Chardonnay Wine
Fermentation
Chandra Richter, PhDE&J Gallo Winery
Metabolomics
March 24, 20143
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Winemaking at the Molecular Level
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AAAAAA
AAA
O O
R
OH OH
R
SGlu
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Winemaking
Water
Sugar
Acids
Minor Components
Water
Ethanol
Glycerol
Acids
Minor Components
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Yeast Metabolism
Sugar
Pyruvic Acid
Acetaldehyde
Ethanol
Glycerol
Diacetyl
TCA CycleAcetic Acid Acetyl CoA
Fatty Acid CoA
Fatty Acids
Monoterpenes
Sulfate
Sulfite
Amino Acids
H2S
Mercaptans
Keto Acids
Aldehydes
Higher Alcohols
Esters
Glycosides Aglycones Cysteines Conjugates
Volatile Thiols
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Identify metabolites present at three stages during fermentation
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PDM
Time (days)
0 2 4 6 8 10
RS
(g/
10
0m
l)
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10
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% A
lcoh
ol
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2
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Cel
l Con
cen
trat
ion
(M
M c
ells
/ml)
0
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RS Alcohol Cell Concentration
Chardonnay Juice
GC-MS, LC-MS-MS
Look for metabolic patterns
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Winemaking Process
• Chardonnay juice• 10 gallon• 65F• Agitation• PDM yeast• Triplicate• Sampled Daily
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Fermentation Timecourse
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Endo- and Exo-Metabolome
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Exo-Metabolome
Endo-Metabolome
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Summary of Metabolites
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PCA Separates Time
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AB
C
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Glycoloysis
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• Early stages of fermentation: sugars are high in the juice and low in the cell.
• Cells are rapidly producing biomass.
• Later stages in fermentation: cells reduce uptake of sugar and glycolysis slows.
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TCA
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• Similar pattern seen in the TCA cycle intermediates.
• a-ketogluterate shows a unique profile
• Deamination of glutamine
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Correlation Profiles
Endometabolome Exometabolome
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Hierarchichal Relationship
16Endometabolome Exometabolome
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Metabolic Flux
Endometabolome Exometabolome
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Sugar
Pyruvic Acid
Acetaldehyde
Ethanol
Glycerol
TCA Cycle
Acetic Acid Acetyl CoA
Fatty Acids
Amino Acids
Keto Acids
Aldehydes
Higher Alcohols
Esters
Regulation of Metabolism
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Conclusions• Identified the endo- and exo-metabolome
over the course of a wine fermentation• Metabolic pathways are differentially
regulated through fermentation– Glycolysis, TCA active during early stages– Amino acid catabolism increases activity at later
stages• Provides understanding of key primary and
secondary metabolites
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Acknowledgements
• Dave Santino• Steven Kukesh• Nick Dokoozlian• Metabolon
– Adam Kennedy– Lining Guo
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Let Us Meet Again
We welcome you all to our future conferences of OMICS Group
International
Please Visit:
www.omicsgroup.comwww.conferenceseries.com
www.metabolomicsconference.com