RH-ISOIMMUNIZATION AND & ABO incompatibility Prepared by N. Bahniy.
ABO/Rh Blood Typing Lab
Transcript of ABO/Rh Blood Typing Lab
![Page 1: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/1.jpg)
ABO/Rh Blood Typing Lab
Lab 8
![Page 2: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/2.jpg)
The purpose of this lab is to perform a slide ABO/Rh blood typing procedure,
discuss the clinical significance of ABO/Rh blood typing, and how
antigens and antibodies relate to the blood typing procedure
![Page 3: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/3.jpg)
Materials
• Whole Blood • Anti-d (Rh) antiserum
![Page 4: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/4.jpg)
• Anti-a antiserum • Anti-b antiserum
![Page 5: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/5.jpg)
• Plastic Pipette
• Rh View Box
![Page 6: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/6.jpg)
• Applicator sticks • Blood Typing slide
![Page 7: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/7.jpg)
• Work mat
(paper towel)
• Laboratory Coat
![Page 8: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/8.jpg)
• Disinfecting Wipes • Biohazard Containers
![Page 9: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/9.jpg)
If doing finger stick
• Alcohol swabs
• Band-Aid
![Page 10: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/10.jpg)
• Lancet • Sterile Cotton
![Page 11: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/11.jpg)
![Page 12: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/12.jpg)
1.) Mix antiserum
![Page 13: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/13.jpg)
2.) Place a drop of anti- a, b, and d(Rh) in the labeled wells on the
blood typing slide
![Page 14: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/14.jpg)
![Page 15: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/15.jpg)
3.) Mix blood well, and place a small drop of blood in each well.
![Page 16: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/16.jpg)
![Page 17: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/17.jpg)
4.) Mix the “a” well with an applicator stick
![Page 18: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/18.jpg)
![Page 19: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/19.jpg)
mix the “b” well with another applicator
stick.
![Page 20: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/20.jpg)
![Page 21: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/21.jpg)
5.) Rotate typing slide for two minutes ans interpret ABO
blood type.
![Page 22: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/22.jpg)
![Page 23: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/23.jpg)
6.) Place slide vertically on the Rh view box, rock slide back and forth on the view box for one
minute, then interpret Rh data.
![Page 24: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/24.jpg)
![Page 25: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/25.jpg)
7.) Replace and dispose of all supplies and equipment
according to instructor (also see lab disposal book). Sheet on wall.
![Page 26: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/26.jpg)
8.) Disinfect top and bottom work area, test tube rack, and
any other item that may be contaminated with body fluids.
![Page 27: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/27.jpg)
Raw Data/ Calculations
• Draw your slide reactions to show agglutination in each well by using the agglutination pictures.
Clumps No Clumps
![Page 28: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/28.jpg)
Normal Ranges:
N/A
![Page 29: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/29.jpg)
Results:
ABO/Rh Blood type
_________________
Look at the pictures on labsheet to help interpret results.
![Page 30: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/30.jpg)
ConclusionState whether your blood type is the most common, the least common or neither, from
which blood type(s) you can receive blood, and to which blood type(s) you can donate blood.
Most common in all ethnic groups is Type OLeast common type in all ethnic groups is Type AB
Types that vary due to ethnic origin are Type A and Type B.
![Page 31: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/31.jpg)
Blood type A / Rh+ :
![Page 32: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/32.jpg)
Blood type B / Rh-:
![Page 33: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/33.jpg)
Blood type AB / Rh+:
![Page 34: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/34.jpg)
Blood type O / Rh-:
![Page 35: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/35.jpg)
Clinical SignificancePeople can receive transfusions of only certain blood types,
depending on the type of blood they have. If incompatible
blood types are mixed, erythrocyte destruction,
agglutination and other problems can occur. By using the
ABO/Rh blood typing, one can find their blood type and
compatablity.
Additionally, the ABO blood groups and other inherited
antigen characteristics of red blood cells are often used in
medico-legal situations involving identification of disputed
paternity.
![Page 36: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/36.jpg)
Questions…Answer using your notes and
text books.
![Page 37: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/37.jpg)
1. What happens if the patient receives the wrong type of blood?
2. Determine the ABO blood type the donor must have to give red cells to each of the following recipients?
Recipient blood type Donor
A _________
B _________
AB _________
O __________
![Page 38: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/38.jpg)
3.) Who is the Universal Donor?
4.) Who is the universal recipient?
5.) Determine the Rh factor of the offspring resulting from each marriage.
Marriage Possible Genotypes Phenotype and Percentages
--and++ ___________ ________________
++and-- ___________ ________________
--and+- ___________ ________________
+-and+- ___________ ________________
![Page 39: ABO/Rh Blood Typing Lab](https://reader031.fdocuments.in/reader031/viewer/2022012019/61688220d394e9041f700e76/html5/thumbnails/39.jpg)
ABO/Rh Blood Typing Lab
Performed by
Ofelia Gamez and Franky Lam
Class of 2009