ABHAY A. SHUKLA, Ph.D.

5720 Forest Park Rd., #6309 abhay.shukla@utsouthwestern.edu

Dallas, TX, 75235 (214)254-1993

PROFESSIONAL SUMMARY

Ph.D. scientist with more than 6 years of experience in targeted gene expression, signal transduction,

identification and characterization of small molecules as potential drugs.

Lead scientist in various projects including high throughput screening of small molecule library (250,000

compounds) to identify potential activators of Bone morphogenetic protein pathway, characterization of PTPRE

as tumor suppressor, depicting role of COMT polymorphism in schizophrenia and transcriptional regulation of

DPP III gene expression.

Editorial board member for peer reviewed journals. Reviewer board member of several journals including

molecular carcinogenesis and Plos one. Have gained experience in timely reviewing research article and

submitting comments.

PROFESSIONAL EXPERIENCE/ TRAINING

Research Scientist: Pediatrics, UT Southwestern Medical Center, Dallas, Texas Aug. 2015-Present

Designed cell line based assay (Luciferase assay), performed screening of a small molecule compound library

(250,000 compounds) and analyzed data to identify potential compounds.

Performed in vitro assays (affinity mediated pull down, co-immunoprecipitation, kinase assay, real time PCR,

western) and in vivo assays (in situ hybridization, immunofluorescence, gene expression) using zebrafish as

model system to validate compounds specificity and identified potential targets of selected compounds.

Generated data for structure activity relationship (SAR) of selected compounds.

Performed work that led to filing patent (in process) of selected chemical compounds.

Postdoctoral Fellow: Pediatrics, UT Southwestern Medical Center, Dallas, Texas Jun. 2011-2015

Gained expertise in studying signal transduction.

Performed kinase array to identify novel substrate of a phosphatase and characterized targets using expression,

mutagenesis, and pull down techniques.

Generated stable cell lines and performed in vitro and in vivo (mouse) cancer progression assays.

CRISPR/cas9 mediated genome engineering, miRNA real time PCR.

Supervised the development of graduate students, clinical fellows, and medical student projects.

Postdoctoral Fellow: Psychiatry, UT Southwestern Medical Center, Dallas, Texas Sep. 2009-May 2011

Initiated and characterized real time PCR to measure expression of several genes using frozen human brain

clinical samples.

Performed in situ hybridization (radiolabeled) and westerns blots to measure expression of genes.

Supervised masters students in completion of project.

Abhay A. Shukla - 2

Ph.D. Biochemistry: All India Institute of Medical Sciences, New Delhi (India) Aug. 2003-2009

Gained experience in studying transcriptional regulation of gene expression using DPPIII gene

Gained extensive experience in cell and tissue culture, recombinant gene expression, transfection, viral mediated

transduction.

Conducted multiple cloning of genes and generated several constructs.

Performed DNA-Protein interaction experiment (EMSA, ChiP) to validate interactions.

Purified recombinant protein using mammalian, insect and bacterial expression system.

Raised polyclonal antibody in rabbit.

Raised monoclonal antibody using hybridoma technology.

M.S. Life Sciences: CSJM University, Kanpur (India) July 2001

B.S. Chemistry & Botany: CSJM University, Kanpur (India) July 1999

Educational Certificates:

Certificate in Research Aug. 2011

Certificate in Scientific Management Aug. 2013

Certificate in Cancer Aug. 2015

ACADEMIC AWARDS & FELLOWSHIPS

Junior Research Fellowship: Council of Scientific and Industrial Research, India Aug. 2003-2005

Senior Research Fellowship: Council of Scientific and Industrial Research, India Aug. 2005-2008

Senior Research Fellowship: Defence Research and Development Organization, India Aug. 2008-2009

Cleared Graduate aptitude test for engineering fellowship exam Sep.2003

INDUSTRIAL WORK EXPERIENCE

Medical science liaison/ Scientist, Atharva Biosciences, New Delhi, India 2008-2009

Interact with clinicians to promot PCR based assay to measure viral load in patients

Amplified and purified viral genes from human clinical samples.

Developed clones that are used as control to measure viral load in patient blood samples.

Characterized several PCR reactions to correctly measure viral copy number in clinical samples.

PUBLICATIONS

1. Shukla AA. High Throughput Screening of Small Molecule Library: Procedure, Challenges and Future. J Cancer

Prev Curr Res, 2016, 5(2): 00154. DOI: 10.15406/jcpcr.2016.04.00154

2. Shukla AA, Jha M, Birchfield T, Mukherjee S, Gleason K, et al. COMT val158met polymorphism and molecular

alterations in the human dorsolateral prefrontal cortex: Differences in controls and in schizophrenia. Schizophr Res.

2016 Mar 24;PubMed PMID: 27021555.

3. Singh AK, Kumar R, Shukla AA, Hariprasad G, Chauhan SS, et al. Identification and molecular characterization of

a novel splice variant of human 5- lipoxygenase gene. Mol Biol Rep. 2014 Dec;41(12):8255-60. PubMed PMID:

25218842.

Abhay A. Shukla - 3

4. Rakheja D, Chen KS, Liu Y, Shukla AA, Schmid V, et al. Somatic mutations in DROSHA and DICER1 impair

microRNA biogenesis through distinct mechanisms in Wilms tumours. Nat Commun. 2014 Sep 5;2:4802. PubMed

PMID: 25190313; NIHMSID: NIHMS616408; PubMed Central PMCID: PMC4159681.

5. Yanagi M, Joho RH, Southcott SA, Shukla AA, Ghose S, et al. Kv31-containing K(+) channels are reduced in

untreated schizophrenia and normalized with antipsychotic drugs. Mol Psychiatry. 2014 May;19(5):573-9. PubMed

PMID: 23628987.

6. Gleason KA, Birnbaum SG, Shukla A, Ghose S. Susceptibility of the adolescent brain to cannabinoids: long-term

hippocampal effects and relevance to schizophrenia. Transl Psychiatry. 2012 Nov 27;2:e199. PubMed PMID:

23188199; PubMed Central PMCID: PMC3565769.

7. Verduzco D, Dovey JS, Shukla AA, Kodym E, Skaug BA, et al. Multiple isoforms of CDC25 oppose ATM activity

to maintain cell proliferation during vertebrate development. Mol Cancer Res. 2012 Nov;10(11):1451-61. PubMed

PMID: 22986406; NIHMSID: NIHMS408604; PubMed Central PMCID: PMC3511848.

8. Singh AK, Singh R, Naz F, Chauhan SS, Dinda A, Shukla AA et al. Structure based design and synthesis of peptide

inhibitor of human LOX-12: in vitro and in vivo analysis of a novel therapeutic agent for breast cancer. PLoS One.

2012;7(2):e32521. PubMed PMID: 22384268; PubMed Central PMCID: PMC3285689.

9. Samaiya M, Bakhshi S, Shukla AA, Kumar L, Chauhan SS. Epigenetic regulation of cathepsin L expression in

chronic myeloid leukaemia. J Cell Mol Med. 2011 Oct;15(10):2189-99. PubMed PMID: 21496199; PubMed

Central PMCID: PMC4394228.

10. Jain M, Bakhshi S, Shukla AA, Chauhan SS. Cathepsins B and L in peripheral blood mononuclear cells of pediatric

acute myeloid leukemia: potential poor prognostic markers. Ann Hematol. 2010 Dec;89(12):1223-32. PubMed

PMID: 20567828.

11. Katara R, Mir RA, Shukla AA, Tiwari A, Singh N, et al. Wild type p53-dependent transcriptional upregulation of

cathepsin L expression is mediated by C/EBPα in human glioblastoma cells. Biol Chem. 2010

Sep;391(9):1031-40. PubMed PMID: 20536385.

12. Shukla AA, Jain M, Chauhan SS. Ets-1/Elk-1 is a critical mediator of dipeptidyl-peptidase III transcription in

human glioblastoma cells. FEBS J. 2010 Apr;277(8):1861-75. PubMed PMID: 20236318.

13. Sansanwal P, Shukla AA, Das TK, Chauhan SS. Truncated human cathepsin L, encoded by a novel splice variant,

exhibits altered subcellular localization and cytotoxicity. Protein Pept Lett. 2010 Feb;17(2):238-45. PubMed PMID:

19663777.

14. Verma R, Gupta RB, Singh K, Bhasin R, Anand Shukla A, et al. Distribution of CCR5delta32, CCR2-64I and

SDF1-3'A and plasma levels of SDF-1 in HIV-1 seronegative North Indians. J Clin Virol. 2007 Mar;38(3):198-203.

PubMed PMID: 17240189.

Google Scholar Citation link: https://scholar.google.com/citations?user=L6IyYDQAAAAJ&hl=en

Book chapter

Shukla AA, Jain M, Chauhan SS. Online chapter entitled “Regulation of gene expression” National Science Digital

library (NSDL at NISCAIR) (http://nsdl.niscair.res.in/handle/123456789/572).

Abhay A. Shukla - 4

NCBI gene bank submission

Shukla, AA, Jain, M. and Chauhan, S.S. Homo sapiens dipeptidyl peptidase III (DPP3) gene, partial sequence

GenBank: FJ793449.1 (http://www.ncbi.nlm.nih.gov/nuccore/FJ793449.1)

Manuscript under review/preparation

1. Abhay A. Shukla, Shama Khokhar, James F. Amatruda, Dinesh Rakheja. Protein Tyrosine Phosphatase Epsilon

(PTPE), a Candidate Tumor-Suppressor in Wilms Tumors of Childhood, can regulate PI3K/AKT/mTOR

pathway. (Under review)

2. Abhay A Shukla, Chuo Chen, James F Amatruda. Identification of small molecule activators of BMP

signaling pathway (Under Preparation)

Abstract of papers published in indexed journals

1. Albert Budhipramono, Dinesh Rakheja, Kenneth S Chen, Nicholas Fustino, Abhay Shukla, Jonathan Wickiser,

Theodore Laetsch, James F Amatruda. EGFR and mTORC1 are novel therapeutic targets in nonseminomatous

germ cell tumors. Cancer Research 74 (20 Supplement), A22-A22

2. Dinesh Rakheja, Kenneth S Chen, Yangjian Liu, Abhay A Shukla, Sara Hildebrand, Vanessa Schmid,

Xiaoyong Sun, Xin Feng, Tsung-Cheng Chang, Shama Khokhar, Nitin J Karandikar, James S Malter, Joshua T

Mendell, James F Amatruda. Somatic mutations in DROSHA and DICER1 impair microRNA biogenesis in

Wilms tumors. Cancer Research, 74, A22-A22, 2014/10/15

3. D. Rakheja, K. Chen, N. Fustino, A. Shukla, J. Wickiser, T. Laetsch, J. Amatruda. EGFR and Mtorc1

signaling pathways are novel targets in pediatrics germ cell tumors. Pediatric blood and cancer 61, S4-S4, 2014.

4. A Shukla, S Khokhar, JF Amatruda, D Rakheja. Protein tyrosine phosphatase epsilon (PTPE), a candidate tumor-

suppressor in Wilms tumors of childhood, can regulate PI3K/AKT/mTOR pathway. CANCER RESEARCH 73 (8),

2013

5. A Shukla, T Birchfield, K Gleason, B Potts, S Ghose. Molecular alterations Molecular alterations associated with

the COMT Val158Met polymorphism in schizophrenia. Schizophrenia bulletin 37, 196-197, 2011

6. S Ghose, K Gleason, A Shukla. Altered expression of metabotropic glutamate receptor proteins in

frontostraital region in schizophrenia. Schizophrenia bulletin 37, 190-190, 2011

7. Kelly Gleason, Abhay Shukla, B Potts, S Birnbaum, Subroto Ghose. Early cannabinoid exposure leads to

Schizophrenia-like behaviors in mice. Schizophrenia bulletin 37, 25-25, 2011

Selected conference/symposium attended

1. Shukla et al., Identification of small molecule activators of BMP signaling pathway. UT Southwestern Medical

Center and the Harold C. Simmons Cancer Center, Dallas,2015

2. Shukla et al., Protein Tyrosine Phosphatase Epsilon (PTPE), a Candidate Tumor-Suppressor in Wilms Tumors

of Childhood, can regulate the PI3K/AKT/mTOR pathway. AACR, Washington D.C., 2013

3. Shukla et al., Molecular alterations associated with the COMT schizophrenia risk gene. 40th

Annual meeting

Society of neurosciences, San Diego, 2010

4. Shukla et al., Truncated human Cathepsin Lencoded by a novel splice variant, exhibits cytotoxicity, heat

Abhay A. Shukla - 5

stability and altered sub-cellular localization. International symposium on Genomic Instability and Cancer-2007

5. Shukla et al., Functional characterization of a novel isoform of Cathepsin L. 33rd

Annual conference of the Indian

immunology society. 28th

-31st

January 2007

6. Shukla et al., Cloning and characterization of human Dipeptidil peptidase III promoter. Association of clinical

biochemists of India. Escort heart institute &research center. December 17-20, 2007

ACADEMIC MEMBERSHIP Society for Neuroscience (SFN, USA)

American Association of Cancer Research (AACR, USA)

American Society of Clinical Oncology (ASCO, USA)

EDITORIAL/REVIEWER BOARD MEMBERSHIP

Editorial board member for BAOJ Allergy & Immunology

Editorial board member for Journal of Biology and Life Science

Editorial board member for journal RNA and Transcription

Editorial board member for journal Gene and Cell Therapy

Reviewer for journal Molecular Carcinogenesis, PLOS ONE, Protein and Peptide Letters, MicroRNA, Edorium

Journal of Biochemistry, Edorium Journal of Neurology, Pediatric Blood & Cancer , Microbiology, Journal of

Global Oncology.

Papers reviewed (Total 16)

British Journal of Medicine and Medical Research: 1

Journal of Biology and Life Science: 5

Endorium Journal of Neurology: 1

Endorium Journal of Biochemistry: 1

MicroRNA: 1

PLOS ONE: 2

Molecular Carcinogenesis: 1

Advances in Biochemistry: 1

International Scholars Journals Publishing Corporation: 3

OUTREACH ACTIVITY, MENTORING AND LEADERSHIP EXPERIENCE

Mentored medical students in laboratory techniques and experimental design 2014, 2015

Visiting Science Teacher: Irma Rangel Middle School, Dallas, TX 2014

Facilitated a bi-weekly multi-lab journal club 2014

Mentored summer students in laboratory techniques and experimental design 2006-2009

Adjunct Professor: Masters Biochemistry class, Kanpur, India 2001-2002

References:

James F. Amatruda, M.D., Ph.D Dinesh Rakheja M.D.

Associate Professor Associate Professor

UT Southwestern Medical Center UT Southwestern Medical Center

James.Amatruda@UTsouthwestern.edu Dinesh.Rakheja@UTsouthwesyern.edu