Discover more at abcam.com/proteins

Our protein range is supported by industry leading

datasheets providing detailed applications and

technical data, bulk options are also available all just

one click away!

Abcam offers a range of over

12,000 proteins from various

expression systems.

Enzymes, cytokines,

human proteins,

His-tagged proteins,

viral proteins and many more...

199_11_JW

Cell differentiation - FGF basic protein (Active)

Human embryonic stem cells were differentiated. BMP-4, FGF-basic

(ab9596) and VEGF (ab9571) were added to direct differentiation. These

images show that CD31 (in green) and CD34 (in red) are abundant and

represent primitive haematopoietic cells. DAPI staining is in blue.

Assays were conducted to compare the bioactivity of human expressed proteins

and their non-human expressed

counterparts such as E.coli expressed,

or other mammalian expressed proteins.

Bioactivity of human expressed IL-4 (ab83686)

Quote our unique promotional code:

PROT-Y7TNA on your next order for 20% off

any of our proteins! The offer is valid from

December 23, 2011 until January 31, 2012.

View terms and conditions at: www.abcam.com/20off

Chimpanzees are complex, long-lived creatures who share 96 percent

of our DNA. Yet while their emotional needs and capacity for suffering

are much like our own, the immunological and physiological differences

between us make them poor models for human disease research.

There are better alternatives. That’s why more than 700 researchers,

scientists and health professionals have signed in support of

efforts to end the use of chimps in harmful research and testing

in the United States, and to provide permanent sanctuary to

chimpanzees currently in laboratories.

Join them. Sign on at humanesociety.org/supportchimps.

a formula for progress

Congratulations to our 2011 winners!

www.gescienceprize.org

GE

,ima

gin

atio

na

tw

ork

,an

dG

Em

on

og

ram

are

trad

em

ark

so

fG

en

era

lEle

ctric

Co

mp

an

y.

©2

01

1G

en

era

lEle

ctric

Co

mp

an

y—

Allrig

hts

rese

rve

d.F

irstp

ub

lishe

dD

ec

.20

11

.

GE

He

alth

ca

reB

io-S

cie

nc

es

AB

,Bjö

rkga

tan

30

,SE

-75

18

4U

pp

sala

,Sw

ed

en

.

Dr. Erez Lieberman Aiden

Grand Prize Winner and

RegionalWinner, North America

Essay: Zoom!

Dr. Eran Eden

Regional Winner,

All Other Countries

Essay: Proteome Dynamics

and Fate the of Individual Cancer

Cells in Response to a Drug

Dr. Felipe Karam Teixeira

Regional Winner, Europe

Essay: Mechanisms of

Transgenerational DNA

Methylation Inheritance

Dr. Tatsuya Tsukahara

Regional Winner, Asia

Essay: CDK Directs the

Chromosome Passenger

Complex to Centromeres for

Chromosome Bi-Orientation

GE Healthcare

Orders (toll-free) 1-877-616-2355 | Technical support (toll-free) 1-877-678-8324 support@cellsignal.com | Inquiries info@cellsignal.com | Environmental Commitment eco.cellsignal.com

www.cellsignal.comfor quality products you can trust...

Antibodies and Related Reagents for Signal Transduction Research

The highest quality antibodies for the study of

Epigenetics from Cell Signaling Technology

:: Innovative products from Cell Signaling Technologyoffer unsurpassed sensitivity, specificity, reproducibility,and performance.

:: Extensive in-house validation means optimization isnot left up to you.

:: Technical support provided by the same scientists whoproduce and validate the products translates into athorough, fast, and accurate response.

©2011

CellS

ignalingTechnology,Inc.

CellS

ignalingTechnology

®,XP

®,andS

impleC

hIP®

trademarks

ofCellS

ignalingTechnology,Inc.

TOP IMAGE: Heterochromatin is characterized by a repressive, tight packaging of nucleosomes, whichimpedes transcription factors from gaining access to regulatory sites on the DNA. Methylation of cytosinebases in regions called CpG islands, found in many gene promoters, leads to formation of transcription-ally repressed heterochromatin. Methylation of cytosine bases by DNA methyltransferases (DNMTs)facilitates recruitment of Methyl-CpG-binding Protein 2 (MeCP2), which brings along other associatedproteins, including histone deacetylases (HDACs), histone methyltransferases (HMTs), and Hetero-chromatin Protein 1 (HP1). These proteins then facilitate the deacetylation and methylation of histoneproteins, resulting in the formation and maintenance of the repressive state of heterochromatin. To viewour epigenetics digital animation movie and for more information, please visit www.cellsignal.com.

MeCP2

HDAC

HP1

DNMT

HMT

0

0.2

1.0

0.6

0.4

0.8

1.4

1.6

1.2

Perc

ent

Input

HoxA2 α Satellite

Ezh2 (D2C9) XP®

Rabbit mAb #5246

Normal RabbitIgG #2729

A

BEzh2 (D2C9) XP® Rabbit mAb #5246:

IHC analysis of para�n-embedded human lymphoma

(A) using #5246. Confocal IF analysis of HeLa cells

(B) using #5246 (green) and S6 Ribosomal Protein

(54D2) Mouse mAb #2317 (blue). Actin filaments

were labeled with DY-554 phalloidin (red). Chromatin

immunoprecipitations (C) were performed with cross-

linked chromatin from 4 x 106 NCCIT cells and either

5 µl of #5246 or 2 µl of Normal Rabbit IgG #2729

using SimpleChIP® Enzymatic Chromatin IP Kit

(Magnetic Beads) #9003. The enriched DNA was

quantified by real-time PCR.

Unparalleled product quality, validation, and technical support

C

1200 New York Ave., NW • Washington, DC 20005 • +1 202-326-6417 • ScienceTranslationalMedicine.org

AAASmember price:

US$52 online only

US$225 print and online (add US$100 for non-US delivery)

AAASmembership number required ______________________________

Nonmember price:US$156 online onlyUS$465 print and online (add US$100 for non-US delivery)

Name ___________________________________________

Address _________________________________________

_________________________________________________

City _____________________________________________

State/Province ____________________________________

Zip/Postal Code ___________________________________

Country __________________________________________

E-mail ________________________________________

Phone ___________________________________________

Payment

Check (payable to AAAS – Science TranslationalMedicine)Mail check and this form to:AAASAttn:Membership Department1200NewYork Avenue, NWWashington, DC 20005 USA

Chargemy:

VISA MasterCard American Express

Card Number________________________________________

Expiration Date______________________________________

Signature___________________________________________

Date _______________________________________________

If paying by credit card, youmay FAST FAX your order to+1 202-842-1065

Subscribe now for 1 year’s access to Science Translational Medicine

(required for subscription activation)

Science Translational Medicine, the newest weekly journal from AAAS,is focused on applications of basic research knowledge to improvehuman health.

The goal of Science Translational Medicine is simple: help the scientificcommunity harness decades of progress in research at the basic leveland translate these biological discoveries into medical advances.

Science Translational Medicine publishes:• Peer-reviewed primary research papers

• Perspectives and reviews on research from basic scienceand clinical viewpoints

• Survey of recent literature and findings in other journals

• Commentary on policy, funding, regulatory issues, and more.

As a AAAS member, add Science Translational Medicine

access for over 60% off of the regular price. Subscribein any of these ways:

• Go to ScienceTranslationalMedicine.org

• Call +1 202-326-6417

• Mail or fax this form with yourpayment to +1 202-842-1065

ScienceTranslationalMedicine

ScienceTranslationalMedicine.org

Weekly journal integrating science and clinical medicine

Prices valid until December 31, 2011.

comand

Scie

• P

• Pa

• S

• C

As aaccein a

• GSitewide access available foryour institution. Recommend asubscription to your library today.ScienceOnline.org/recommend

ORDER 888.999.1371 • TECH 888.810.6168 • INQUIRIES info@eBioscience.com • WEB www.eBioscience.com

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Full Spectrum AntibodiesTested on relevant biological models, broad �uorophore

formats for most cytometers, and convenient test size options

High Performance ImmunoassaysFlexible for any work�ow situation – multiplexed data, high

sensitivity or value-driven assays

Translational ContentFluorophore-conjugated analytes optimized for the

immunohistochemical identi�cation of in�ltrating immune

cells in tumors

As a leading supplier of immunological

reagents, eBioscience delivers product

quality and value across a variety of

applications, color formats and areas

of biological research. Our expertise in

developing and maintaining standards

of excellence assures our customers

consistency and convenience through-

out our product portfolio.

All Together Now…

Follow us, engage with us and challenge us to support your studies.

New Products, Services, and Solutions

SCIENCE

LA

B S

OLU

TIO

NS

Make theright move!

MPC-200

Multi-manipulator system

Versatile:

Expandable:

Stable:

Doubly Quiet:

PHONE: 415.883.0128 | FAX: 415.883.0572EMAIL: INFO@SUTTER.COM | WWW.SUTTER.COM

User friendly interface controls up to two manipulatorswith one controller. Select components to tailor asystem to fit your needs.

Daisy chain a second controller and operate up tofour manipulators with one input device.

Stepper motors and cross-rolled bearings quaranteereliable, drift-free stability.

Linear stepper-motor drive reduces electrical noise.Thermostatically-controlled coolingfans barely whisper.

The completeScience archive1880–1996

Fully integrated withScienceOnline(1997–today)

Information:www.sciencemag.org/classic

Available to institutionalcustomers through a site license.Contact ScienceClassic@aaas.org

for a quote.

©2007

Jup

ite

rIm

ag

es

Co

rpo

rati

on

A Journal with Impact fromAAAS, the publisher ofScience

Science Translational MedicineIntegrating Medicine and ScienceA recent journal article features the sequencing of fetal DNA from plasma

of a pregnant woman to permit prenatal, noninvasive genome-wide

screening to diagnose fetal genetic disorders.Sci Transl Med 8 December 2010:Vol. 2, Issue 61, p. 61ra91DOI: 10.1126/scitranslmed.3001720

Recommend aninstitutional subscriptionto your library today!ScienceOnline.org/recommend

ScienceTranslationalMedicine.org

Indexed inMEDLINE/PubMed

QuantStudio™ 12K Flex Real-Time PCR SystemDo what you never thought possible. With 5 interchangeable blocks, you can analyze

from 1 to over 12,000 data points per run in any assay format that is right for your project.

The QuantStudio™ 12K Flex system transforms the leading real-time PCR platforms into a

single platform combining maximum throughput, scalability, and flexibility. Now, targeted

discovery, confirmation, high-throughput screening, and everything in between, can be

done on a single system.

See what the system can do at www.appliedbiosystems.com/quantstudio

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

© 2011 Life Technologies Corporation. All rights reserved.

The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. CO124128 1011

within reachproven qPCR performance is now

Built on 20 years of industry-leading innovation, only the Applied Biosystems® real-time

PCR portfolio offers a solution for every lab. From the simple reliability of the StepOne™

family to the unprecedented throughput of the QuantStudio™ 12K Flex system, no other

lineup canmatch our flexibility, scalability, and performance.

©2011 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their

respective owners. TaqMan is a registered trademark of Roche Molecular Systems, Inc. For research use only. Not intended for any animal or human

therapeutic or diagnostic use. CO31297 1211

Shop now atwww.appliedbiosystems.com/qpcr

The best-in-class qPCR platforms are also the best in value

SMALL ANIMAL IMAGING SYSTEMThe In-Vivo Xtreme is a new, multimodal imaging product dedicated to preclinical research. Beneath

its sleek, contemporary design, Xtreme is powered by an innovative system architecture that provides

the ultimate combination of sensitivity, speed, and fl exibility to meet the most demanding challenges

and broadest range of applications for molecular imaging. One of the most compelling features of

this new system’s architecture is the ability to precisely match camera choice with research needs,

performance criteria, and budget. Xtreme currently comes in two camera confi gurations. The front-

illuminated 16 megapixel camera is ideally suited for applications that demand high resolution, while

the back-illuminated 4 megapixel camera excels at very low light imaging experiments. With this

uniquely fl exible design, researchers will have the ability to upgrade their camera as new technology

evolves and research requirements change. Xtreme comes with MI Software, a powerful suite of tools

for acquisition, visualization, and precise quantifi cation of imaging data.

Carestream

For info: 877-747-4357 www.carestream.com

1735www.sciencemag.org/products SCIENCE VOL 334 23 DECEMBER 2011

Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are

featured in this space. Emphasis is given to purpose, chief characteristics, and availability of products and materials. Endorsement by Science or AAAS of any products or

materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier.

Electronically submit your new product description or product literature information! Go to www.sciencemag.org/products/newproducts.dtl for more information.

LIFE SCIENCE TECHNOLOGIES

NEW PRODUCTS

Produced by the Science/AAAS Custom Publishing Office

PCR SYSTEMThe Applied Biosystems QuantStudio 12K Flex Real-Time poly-

merase chain reaction (PCR) system is a highly versatile genetic

analysis instrument that enables cost-effective and effi cient inter-

rogation of hundreds of target genes over thousands of samples in

a single run. The growing demand for biomarker screening experi-

ments, particularly in the early research phases of pharmacogenom-

ics and agricultural biotechnology fi elds, has created a greater need

for high throughput technology that can provide answers for re-

searchers faster, more accurately, and at a reasonable price per reac-

tion. The QuantStudio 12K Flex system addresses this with its ability

to generate more than 12,000 high-quality, TaqMan data points per

run, or up to 110,000 data points in an eight-hour workday. Users can

also run digital PCR experiments on the instrument using nanofl uidic

consumables and dedicated analysis software for increased sensitiv-

ity and specifi city.

Life Technologies

For info: 800-955-6288 www.lifetechnologies.com

FTIR SPECTROMETERThe Fourier transform infrared (FTIR) spectroscopy instrument,

FlowIR, is one of the smallest FTIR instruments on the market.

FlowIR can be placed nearly anywhere along a reactor setup. The

seamless integration of FlowIR into just about any home-built or

commercially available fl ow chemistry synthesis system gives small-

scale researchers control over fl ow reactions comparable to that of

the largest manufacturing conglomerates—while allowing larger or-

ganizations to gather more data along complex multistep reaction

chains. Because no special connections are required, FlowIR allows

real-time fl ow chemistry monitoring at any point along a reaction

chain. Multiple units along the reaction path in multistep synthesis

setups give critical feedback to allow ongoing reaction adjustments.

The enhanced control over potentially volatile reactions enhances

safety. Typically, fewer experiments are also required, because

reagents or other materials can be added as needed. This elimi-

nates the need to perform reactions repeatedly and results in less

material used.

Mettler Toledo

For info: 800-638-8537 www.mt.com

GENE TRANSFECTIONThe magnefect-nano II is a new, compact device that offers gene

transfection with improved effi ciencies and cell viabilities, even with

a range of diffi cult cell types such as neuronal and primary cells.

It uses a unique technology applying proprietary magnet confi gu-

rations and oscillating magnet arrays, which can operate at user-

defi nable variable frequencies, to promote magnetic nanoparticle/

DNA uptake into cells to improve nonviral gene transfection, while

maintaining high cell viability. The device provides improved trans-

fection effi ciency and effectiveness over the best currently available

cationic lipid agents at short transfection times (<30 minutes). It al-

lows retransfection as well as adherent state transfection for user

fl exibility. It offers full fl exibility with interchangeable 6-well, 24-well,

and 96-well array formats and variable oscillating frequency capabil-

ity to enable researchers to optimize protocols specifi cally for the

cell types they are using.

nanoTherics

For info: +44-1782-555-639 www.nanotherics.com

HIGH THROUGHPUT CENTRIFUGEThe Allegra X-14 is a 3 L benchtop centrifuge that accepts a variety

of tubes and adapters to provide reliable performance in a range of

applications. Refrigerated and constant-temperature models are of-

fered individually or in prebundled packages that provide everything

needed for quick setup in the laboratory. With a fi xed-angle rotor, the

X-14 provides a maximum speed of 10,200 rpm and delivers rcf of

11,400 × g. Swinging-bucket rotors can achieve a maximum speed

of 4,300 rpm and rcf of 4,300 × g and, utilizing a wide selection

of tubes, bottles, and plates, can accommodate a range of sample

types and volumes. Depending on rotor and labware, the Allegra

X-14 can process as many as 148 tubes per cycle. The refrigerated

model offers a temperature range of 2°C to 40°C and features a ro-

bust cooling system that brings samples from room temperature

to 4°C in less than four minutes, reducing wait time and enabling

runs to be processed effi ciently. The constant-temperature model is

preset to 20°C.

Beckman Coulter

For info: 800-742-2345 www.beckmancoulter.com

Substantial financialassistance is availablefor most courses.

2012Cutting-EdgeScience Training

Analytical &Quantitative LightMicroscopyMay 9 – May 18

Biology ofParasitism: ModernApproachesJune 15 – August 4

BioMedicalInformaticsSponsored by the NationalLibrary of Medicine1st Session: May 27 – June 22nd Session: September 16– September 22

Embryology:Concepts &Techniquesin ModernDevelopmentalBiologyJune 2 – July 15

Frontiers inReproduction:Molecular &Cellular Concepts& ApplicationsApril 28 – June 10

Frontiers inStem Cells &RegenerationSeptember 30 – October 6

Fundamental Issuesin Vision ResearchSponsored by the NationalEye InstituteAugust 5 – August 18

Gene RegulatoryNetworks forDevelopmentOctober 9 – October 20

Methods inComputationalNeuroscienceJuly 29 – August 26

Microbial DiversityJune 9 – July 26

Molecular Biologyof AgingSponsored by the EllisonMedical FoundationJuly 22 – August 11

MolecularMycology: CurrentApproachesto FungalPathogenesisAugust 1 – August 17

Neural Systems& BehaviorJune 2 – July 29

NeurobiologyJune 2 – July 29

NeuroinformaticsAugust 11 – August 26

Optical Microscopy& Imaging in theBiomedical SciencesSeptember 27 – October 7

Physiology: ModernCell Biology UsingMicroscopic,Biochemical andComputationalApproachesJune 9 – July 29

Strategies andTechniquesfor AnalyzingMicrobialPopulationStructuresAugust 1 – August 11

Summer Programin Neuroscience,Ethics, & Survival(SPINES)June 16 – July 14

Teaching AboutNeurobiology ofBrain DysfunctionAugust 12 – August 19

Workshopon MolecularEvolutionJuly 22 – August 1

ZebrafishDevelopment& GeneticsAugust 5 – August 19

MBL courses derive

major support from grants

awarded by Howard Hughes

Medical Institute,the

Burroughs Wellcome

Fund, and the National

Institutes of Health.

FOR APPLICATION FORMSAND INFORMATION ABOUT

MBL COURSES CONTACT:

Admissions Coordinator

MBL, 7 MBL Street

Woods Hole, MA 02543

508.289.7401

admissions@mbl.edu

mbl.edu/education

MBL is an EEO/AffirmativeAction Institution.

Founded in 1888 as theMarine Biological Laboratory

mbl.edu/education

©2011 Life Technologies Corporation. All rights reserved. The trademarksmentioned herein are the property of Life Technologies Corporation or their

respective owners, unless otherwise noted. For research use only. Not intended for any animal or human therapeutic or diagnostic use. CO22940 0511

headline essenceheadline brow

the difference between

paradigm and save a dime

Go to www.invitrogen.com/discovergibcoGet the free mobile app for your phone at http://gettag.mobi

Confidence leads to discovery.Your inspiration, skill, and uncompromising dedicationmove us toward a better future.

Gibco® GlutaMAX™media and fetal bovine serum enable you to achieve consistent results

worthy of your research.

Gibco. Every little thingmatters.

a dimeime

©2011 Life Technologies Corporation. All rights reserved. The trademarksmentioned herein are the property of Life Technologies Corporation or their

respective owners. For research use only. Not intended for any animal or human therapeutic or diagnostic use. CO22940 0611