AAV9-CMV-EGFPmiR122(3x)TS

AAV9-CMV-EGFP

AAV9-CMV-MLC0.26-EGFPmiR122(3x)TS

AAV9-CMV-MLC0.26-EGFP

hea

rt

Supplementary Figure I

Qu

adri

cep

sfe

mo

ris

mu

scle

AAV9-CMV-EGFPmiR122(3x)TS

AAV9-CMV-EGFP

AAV9-CMV-MLC0.26-EGFPmiR122(3x)TS

AAV9-CMV-MLC0.26-EGFP

Supplementary Figure II

AAV9-CM

V-

EGFP m

iR12

2(3x

)TS

AAV9-CM

V-

EGFP

AAV9-CM

V-MLC0.

26-

EGFP m

iR12

2(3x

)TS

AAV9-CM

V-

MLC0.

26-E

GFP

AAV9-CM

V-

EGFP m

iR12

2(3x

)TS

AAV9-CM

V-

EGFP

AAV9-CM

V-MLC0.

26-

EGFP m

iR12

2(3x

)TS

AAV9-CM

V-

MLC0.

26-E

GFP

Supplementary Figure III

heart

3.0

4.0

5.0

6.0

7.0

8.0

9.0

10.0

11.0

12.0

13.0

C

t (v

ec

tor

vs

. G

AP

DH

)

liver

0.0

1.0

2.0

3.0

4.0

5.0

6.0

7.0

8.0

9.0

10.0

C

t (v

ec

tor

vs

. G

AP

DH

)

Supplementary Figure Legends

Supplementary Figure I:

Regulation of AAV9 vector mediated gene expression by miR122 in the heart does not affect

transduction of cardiomyocytes. Representative microphotographs show colocalization of EGFP

fluorescence with an actin stain using phalloidin (red). The pattern of EGFP expression in the heart is similar

between all groups irrespective of the use of miR122 TS. Bar: 50 µm.

Supplementary Figure II:

Visualization of EGFP expression in skeletal muscle. Cryosections of quadriceps femoris muscle from

adult mice 4 weeks after intravenous injection of 7.5x1011 vg of AAV9-CMV-EGFP, AAV9-CMV-EGFPmiR122(3x)TS,

AAV9-CMV-MLC0.26-EGFP, and AAV9-CMV-MLC0.26-EGFPmiR122(3x)TS were compared. Expression was found

to be close to the detection limit. No difference in expression was detectable either with or without miR122 TS

in combination with the CMV or CMV-MLC0.26 promoter. Bar: 50 µm

Supplementary Figure III:

Comparison of AAV vector DNA amount in vivo. Vector DNA of AAV9-CMV-EGFP, AAV9-CMV-

EGFPmiR122(3x)TS, AAV9-CMV-MLC0.26-EGFP and AAV9-CMV-MLC0.26-EGFPmiR122(3x)TS in heart and liver was

quantified by real-time PCR and related to GAPDH DNA. Shown are Ct values of vector DNA vs. GAPDH

DNA, the mean and standard error of mean (SEM). The vector DNA levels measured in the heart and the liver

were very similar between all groups.