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A Perfusion-based Micro 3-D Cell Culture Platform NTHU MSCL Adviser: C. H. Liu C. He Yeh

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A Perfusion-based Micro 3-D Cell Culture Platform Outline Culture environment at large scale Culture environment at micro scale Principle Fabrication Conclusion & Features Further work

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General Culture Equipment (1) (2) (3) (4) (5) (6) (7) (8) (9) (10) (11) (12) (13) (14) (15) Lab On a Chip

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On Chip Advantages Small dimension Saving sample Rapid assay process (micro dimension, avoid medium or reagent diffusion time gradient or concentration different ) Cheap, disposable

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On Chip Culture Requirement Temperature : 37 pH : 7.2 ~ 7.4 ( 95% air 5%CO 2 or HEPES) Buffered saline (PBS, PBSA, etc) Medium : (Serum, glucose, amino acids, vitamin, antibiotics ) 3D culture (In vivo Culture)

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A Perfusion-based Micro 3-D Cell Culture Platform

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Working Principle :

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A Perfusion-based Micro 3-D Cell Culture Platform Fabrication :

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Conclusion Feature 1: 3D well-defined culture environment mimic an in vivo environment distinct cellular behaviors (cell-cell and cell-extracellular interactions ) provide more meaningful data

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Conclusion Feature 2 : Short Loading cell channel Feature 3 : High throughput (8 chambers)

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Further work Bioassay (inject reagent instead of culture medium in vivo bioassay in vivo bioassay) Biochemical experiment Tissue engineering Drug screening TAS

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Reference A PERFUSION-BASEDMICRO 3-D CELL CULTURE PLATFORM S. B. Huang1, M. H. Wu2, Z. F. Cui3, Z. Cui2, J. L. Lin4 and G. B. Lee1* MICROFLUIDIC DEVICES FOR THE ASSEMBLY AND CULTURE OF THREE-DIMENSIONAL MULTI- CELLULAR CONSTRUCTS WITH DIFFUSION-LIMITED MICROENVIRONMENTS M. Pinelis1, R. W. Kasinskas2, R. T. Borno1, J. H. Park1, E. Chu3, N. S. Forbes2 and M. M. Maharbiz1 "Differentiation of human Embryonic Stem Cells into Hepatocytes in 2D and 3D culture system in vitro" The International Journal of Developmental Biology, Vol. 50, pp. 645-652, 2006 "The multilayered postconfluent cell culture as a model for drug screening" Critical Reviews in Oncology/Hematol, Vol. 36, pp. 147-157, 2000. J. M. Pardon; C.L. van der Wilt; K. Smid; E. Smitskamp-Wilms; H.H. Baukus; P. E. Piazo; G. "Development of PDMS Microbioreactor with Well-defined and Homogenous Culture Environment for Chondrocyte 3-D Culture M. H. Wu, J. P. G. Urban, Z. Cui and Z. F. Cui, Biomedical Microdevices, Vol. 8, pp. 331-340, 2006.