A Novel Extracellular Vesicle Isolation Method Used to Discover … · 2020. 7. 23. · Anna...
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Anna Markowska Ph.D., [email protected] Shannon Pendergrast Ph.D., [email protected] Contact Information A Novel Extracellular Vesicle Isolation Method Used to Discover Urine Liver Disease Candidate Biomarkers P. Shannon Pendergrast 1 , Anna Markowska 1 , Ashok P. Reddy 2 , Phillip Wilmarth 2 , Scott Naugler 3 , Yiyi Chen 4 , Lina Gao 4 , Thuy Ngo 5 Charles R. Thomas, Jr. 6 , Christie Binder 6 1. Ymir Genomics Cambridge MA, 2. Proteomics Shared Resources, OHSU, Portland, Oregon, 3. Division of Gastroenterology and Hepatology, OHSU, Portland, Oregon. 4. Department of Biostatistics, OHSU, Portland, Oregon.. 5. Knight CEDAR Technology, OHSU, Portland, Oregon 6. Radiation Medicine, Knight Cancer Institute, OHSU, Portland, Oregon The Need for Better HCC Surveillance Screening Urine EVs as a Convenient Source of Biomarkers: Potential and Challenges Liver Disease Pilot Study Design The Std of Care for HCC Surveillance, Ultrasound + AFP is inadequate due to low Sensitivity, a lack of widespread availability/expertise, and inconvenience. What is needed is a convenient screen for early HCC. Ymir Genomics and OHSU have performed a pilot discovery study for urine EV-associated protein and miRNA liver disease biomarkers. Several interesting candidate biomarkers have been identified. Furthermore, the study has perfected methods for sample procurement, sample preparation of EV-associated biomolecules, and high-throughput biomolecule identification with MS and miRNA array. Follow-up larger scale studies are now being planned HCC is a Growing Problem Ymatrix Overcomes the Challenges of Urine for EV Isolation with a Rapid, Low-cost Protocol Immunoblot and Mass Spectrometry data miRNA Array Data (Abcam Array) Proof-of-Concept: Known and Novel Liver- selective or Liver Disease Biomarkers in Patient Urinary Ymatrix EV Preps Two Early Candidate miRNA HCC Biomarkers have been identified CD9 Normalized Candidate Levels in Pooled Group Urinary EV Preps
Transcript of A Novel Extracellular Vesicle Isolation Method Used to Discover … · 2020. 7. 23. · Anna...
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Anna Markowska Ph.D., [email protected] Pendergrast Ph.D.,
Contact Information
A Novel Extracellular Vesicle Isolation Method Used to Discover Urine Liver Disease Candidate Biomarkers
P. Shannon Pendergrast1, Anna Markowska1, Ashok P. Reddy2, Phillip Wilmarth2, Scott Naugler3, Yiyi Chen4, Lina Gao4, Thuy Ngo5 Charles R. Thomas, Jr.6, Christie Binder6
1. Ymir Genomics Cambridge MA, 2. Proteomics Shared Resources, OHSU, Portland, Oregon, 3. Division of Gastroenterology and Hepatology, OHSU, Portland, Oregon. 4. Department of Biostatistics, OHSU, Portland, Oregon.. 5. Knight CEDAR Technology, OHSU, Portland, Oregon 6. Radiation Medicine, Knight Cancer Institute, OHSU, Portland, Oregon
The Need for Better HCC Surveillance Screening
Urine EVs as a Convenient Source of Biomarkers: Potential and Challenges
Liver Disease Pilot Study Design
The Std of Care for HCC Surveillance, Ultrasound + AFP is inadequate due to low Sensitivity, a lack of widespread availability/expertise, and inconvenience. What is needed is a convenient screen for early HCC.
Ymir Genomics and OHSU haveperformed a pilot discovery studyfor urine EV-associated proteinand miRNA liver diseasebiomarkers.
Several interesting candidatebiomarkers have been identified.Furthermore, the study hasperfected methods for sampleprocurement, sample preparationof EV-associated biomolecules,and high-throughput biomoleculeidentification with MS and miRNAarray. Follow-up larger scalestudies are now being planned
HCC is a Growing Problem Ymatrix Overcomes the Challenges of Urine for EV Isolation with a Rapid, Low-cost Protocol
Immunoblot and Mass Spectrometry data
miRNA Array Data (Abcam Array)
Proof-of-Concept: Known and Novel Liver-selective or Liver Disease Biomarkers in
Patient Urinary Ymatrix EV Preps
Two Early Candidate miRNA HCC Biomarkers have been
identified
CD9 Normalized Candidate Levels in Pooled Group Urinary EV Preps
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