A Guide to Microscopy. The Light Microscope Purpose: History Flea glasses Anton van Leeuwenhoek...
-
Upload
robyn-mckinney -
Category
Documents
-
view
216 -
download
0
Transcript of A Guide to Microscopy. The Light Microscope Purpose: History Flea glasses Anton van Leeuwenhoek...
![Page 1: A Guide to Microscopy. The Light Microscope Purpose: History Flea glasses Anton van Leeuwenhoek Robert Hooke.](https://reader030.fdocuments.in/reader030/viewer/2022032605/56649e745503460f94b74dc8/html5/thumbnails/1.jpg)
A Guide to Microscopy
![Page 2: A Guide to Microscopy. The Light Microscope Purpose: History Flea glasses Anton van Leeuwenhoek Robert Hooke.](https://reader030.fdocuments.in/reader030/viewer/2022032605/56649e745503460f94b74dc8/html5/thumbnails/2.jpg)
The Light Microscope
Purpose: History
Flea glasses Anton van Leeuwenhoek Robert Hooke
![Page 3: A Guide to Microscopy. The Light Microscope Purpose: History Flea glasses Anton van Leeuwenhoek Robert Hooke.](https://reader030.fdocuments.in/reader030/viewer/2022032605/56649e745503460f94b74dc8/html5/thumbnails/3.jpg)
Anton “The Great” Van Leeuwenhoek
Father of microscopy
Apprenticeship Created the first
light microscope
![Page 4: A Guide to Microscopy. The Light Microscope Purpose: History Flea glasses Anton van Leeuwenhoek Robert Hooke.](https://reader030.fdocuments.in/reader030/viewer/2022032605/56649e745503460f94b74dc8/html5/thumbnails/4.jpg)
Robert Hooke
First to see cells
Observed cells in cork
![Page 5: A Guide to Microscopy. The Light Microscope Purpose: History Flea glasses Anton van Leeuwenhoek Robert Hooke.](https://reader030.fdocuments.in/reader030/viewer/2022032605/56649e745503460f94b74dc8/html5/thumbnails/5.jpg)
The Compound Light Microscope
Uses 2 or more lenses to view a sample Objective lens Ocular lens
Light Source Advantages and Limitations
![Page 6: A Guide to Microscopy. The Light Microscope Purpose: History Flea glasses Anton van Leeuwenhoek Robert Hooke.](https://reader030.fdocuments.in/reader030/viewer/2022032605/56649e745503460f94b74dc8/html5/thumbnails/6.jpg)
![Page 7: A Guide to Microscopy. The Light Microscope Purpose: History Flea glasses Anton van Leeuwenhoek Robert Hooke.](https://reader030.fdocuments.in/reader030/viewer/2022032605/56649e745503460f94b74dc8/html5/thumbnails/7.jpg)
Parts of Interest
See homework for functions Diaphragm Revolving nose piece Course adjustment knob Fine adjustment knob Which knob to use during high
power?
![Page 8: A Guide to Microscopy. The Light Microscope Purpose: History Flea glasses Anton van Leeuwenhoek Robert Hooke.](https://reader030.fdocuments.in/reader030/viewer/2022032605/56649e745503460f94b74dc8/html5/thumbnails/8.jpg)
Magnificaiton
Definition: Eye Piece: _____ Scanning Power: ____ Low Power: ____ High Power: ____ How do you calculate total
magnification?
![Page 9: A Guide to Microscopy. The Light Microscope Purpose: History Flea glasses Anton van Leeuwenhoek Robert Hooke.](https://reader030.fdocuments.in/reader030/viewer/2022032605/56649e745503460f94b74dc8/html5/thumbnails/9.jpg)
Resolution
Definition:
Bad Microscope Good Microscope
![Page 10: A Guide to Microscopy. The Light Microscope Purpose: History Flea glasses Anton van Leeuwenhoek Robert Hooke.](https://reader030.fdocuments.in/reader030/viewer/2022032605/56649e745503460f94b74dc8/html5/thumbnails/10.jpg)
Limitations of Light Microscope
Poor Magnification Poor Resolution WHY???
![Page 11: A Guide to Microscopy. The Light Microscope Purpose: History Flea glasses Anton van Leeuwenhoek Robert Hooke.](https://reader030.fdocuments.in/reader030/viewer/2022032605/56649e745503460f94b74dc8/html5/thumbnails/11.jpg)
Stereomicroscope
Dissecting microscope: Why? Reflects light off of the surface Results in a ___ Image
![Page 12: A Guide to Microscopy. The Light Microscope Purpose: History Flea glasses Anton van Leeuwenhoek Robert Hooke.](https://reader030.fdocuments.in/reader030/viewer/2022032605/56649e745503460f94b74dc8/html5/thumbnails/12.jpg)
Electron Microscopes
2 types: Transmission Electron Microscope
(TEM) Scanning Electron Microscope (SEM)
Electrons either pass through a specimen or bounce off of the surface to provide an image
Resolution and Magnification?
![Page 13: A Guide to Microscopy. The Light Microscope Purpose: History Flea glasses Anton van Leeuwenhoek Robert Hooke.](https://reader030.fdocuments.in/reader030/viewer/2022032605/56649e745503460f94b74dc8/html5/thumbnails/13.jpg)
![Page 14: A Guide to Microscopy. The Light Microscope Purpose: History Flea glasses Anton van Leeuwenhoek Robert Hooke.](https://reader030.fdocuments.in/reader030/viewer/2022032605/56649e745503460f94b74dc8/html5/thumbnails/14.jpg)
Images obtained from EM’s
![Page 15: A Guide to Microscopy. The Light Microscope Purpose: History Flea glasses Anton van Leeuwenhoek Robert Hooke.](https://reader030.fdocuments.in/reader030/viewer/2022032605/56649e745503460f94b74dc8/html5/thumbnails/15.jpg)
Electron Microscopes
Advantages? Limitations?