XAC NH HAM LNG AFLATOXIN TRONG THC PHAM

Mot so oc to vi nam thng gap trong t nhien

Cau truc mot so aflatoxin pho bien trong t nhien

Aspergillus flavus toxin Aflatoxin Aflatoxin UV Blue Aflatoxin B Aflatoxin UV Green Aflatoxin G

Tnh oc cua aflatoxinTnh oc cua aflatoxin giam dan t B1>G1>B2>G2, la do oc tnh cua aflatoxin lien quan vi noi oi tai vong furan

Gii han ham lng aflatoxin trong thc pham s dung cho ngiV tnh chat oc cua aflatoxin nen nhieu nc a a ra tieu chuan ve ham lng aflatoxin trong thc pham vi ham lng cho phep t 0 g/kg en 50 g/kg.

CAC PHNG PHAP KIEM TRA D LNG AFLATOXIN TRONG MAU THC PHAM

Phng phap sac ky ban mong - Aflatoxin c chiet bang dung moi thch hp - Dung dch mau kiem tra va chuan aflatoxin vi cac nong o khac nhau c cham len ban sac ky - Ket qua c oc tren en huynh quang tai bc song 365nm - Luc nay ngi ta se phun dung dch H2SO4, neu ung la aflatoxin B1 th iem phat huynh quang se chuyen t mau xanh sang vang

Phng phap cot sac ky m

(Romer Minicolumn Method)

Phng phap huynh quang

-Aflatoxin chiet, lam sach bang cot sac ky ai lc

-Kiem tra tren may huynh quang-Tnh toan ham lng aflatoxin trong mau

Phng phap ELISA canh tranh

Them aflatoxin gan men vao gieng ELISA

Them chuan va mau aflatoxin vao giengU phien

Ra phien ELISA

Lam kho phien tren giay tham

Them c chat hien mau

oc phan ng tren may oc ELISA

Nguyen tac cua phan ng ELISA

Phng phap sac ky long cao ap

- Lm sch mu - Phn tch trn HPLC-FL, LC MS, LC MS/MS

- Lm sch mu bng ct tch chit pha rn SPE C18. - Lm sch mu bng ct Mycosep. - Lm sch mu bng ct sc k i lc min dch

LM SCH MU BNG CT SPE C18-Chit mu bng methanol / acetonitrile.

- Cho mu qua ct C18.- Ra gii aflatoxin ra khi ct bng acetonitrile/ methylenchloride.

- Phn tch trn HPLC.

LM SCH MU BNG CT MYCOSEP

LM SCH MU BNG CT SC K I LC

- Chit mu bng methanol/acetonitrile - Cho mu qua ct sc k i lc

CC BC TINH SCH MU AFLATOXIN BNG CT SC K I LC MIN DCH

Cot sac ky ai lc gan khang the khang aflatoxin

Cho mau qua cot sac ky ai lc.

Aflatoxin gi lai tren cot chat tap nhiem thai loai ra ngoai

Ra giai aflatoxin ra khoi cot sac ky ai lc

PHN TCH AFLATOXIN TRN HPLC

- Phng php o dng h thng sc klng vi u d hunh quang:

- Phng php to dn xut trc ct.- Phng php to dn xut sau ct.

CC PHN NG DN XUT TRONG PHN TCH AFLATOXIN

Dn xut trc ct vi Trifluro-acetic-acid (TFA)

Aflatoxin B1 aflatoxin B2a Aflatoxin G1 aflatoxin G2a

SC K CA AFLATOXIN DN XUT TRC CT

-Dn xut sau ct vi iot.

- Dn xut sau ct vi PBPB: Pyridinium Bromide PerBromide

H THNG TO DN XUT SAU CT BNG HA HC

H THNG TO DN XUT SAU CT BNG HA HC

Dn xut sau ct dng n UV

H THNG TO DN XUT BNG UV

H THNG TO DN XUT BNG UV

PHN TCH AFLATOXIN BNG K THUT KHI PH MT LN LC MS- Khi lng trn in tch (m/z) ca aflatoxin

PHN TCH AFLATOXIN BNG K THUT KHI PH MT LN LC MS

PHN TCH AFLATOXIN BNG K THUT KHI PH MT LN LC MS

PHN TCH AFLATOXIN BNG K THUT KHI PH MT LN LC MS

PHN TCH AFLATOXIN BNG K THUT KHI PH HAI LN LC MS/MS- Khi lng trn in tch (m/z) ca phn mnh aflatoxin

PHN TCH AFLATOXIN BNG K THUT KHI PH HAI LN LC MS/MS

PHN TCH AFLATOXIN BNG K THUT KHI PH HAI LN LC MS/MS