A Comprehensive Review Study Of Antioxidant Potential of Dillenicea Family

A Comprehensive Review Study Of Antioxidant Potential of Dillenicea Family

Presented by : Exam Roll No:M-10203Registration No: 0407Session : July 2015

University of Science and Technology Chittagong (USTC)Department of Pharmacy

Introduction Various human diseases have oxidative stress as one of their

component. Many herbs have been reported to exhibit properties

that combat oxidative stress through their active constituents such

as flavonoids, tannins, phenolic compounds etc. Different Plants of

Dillenicea family has been shown in in vitro experiments to be

endowed with antioxidant activity. Therefore this study was carried

out to evaluate Dillenicea family for its antioxidant activity.

A lot of modern medicines have been discovered as a result of the scientific follow- up of the traditional herbal preparations.

Still there is no cure for the treatment of diseases caused by free radicals like Alzheimer’s disease, cancer etc.

Investigation of the bioactive principles of antioxidant properties with less side effects is very essential to make the drug affordable to local people with low price.

Plants List of Dillenicea family

1Pentagyna Roxb2 Tetracera Sarmentosa3 Dillenia indica L.

Ref: http://ethnobotanybd.com/index.php?action=Taxonomy&key=sci

PLANT PROFILE REVIEWDillinia Indica L.


Pentagyna Roxb.


Determination of antioxidant and analgesic activities of

Different Plants of Dillenicea family

Antioxidant Testa)DPPH radical scavenging b)Reducing Powerc)Total Phenol andd) Total Flavonoid content

AIM AND OBJECTIVES

MATERIALS AND METHODSAntioxidant properties

Antioxidant activity determined by DPPH, reducing power and total antioxidant. Secondary metabolite content determined bySpectrophotometric process.

Reagents :1. Potassium ferricyanide [K3Fe(CN)6] 2. Trichloro Acetic acid 3. Ferric Chloride (FeCl3)4. Phosphate buffer [K2HPO4 + KH2PO4]5. Ascorbic acid (Analytical or Reagent

grade) 6. Water bath7. Centrifuge machine8. Pipette (1-10 ml)

9. DPPH10. UV spectrophotometer

Ref :Clarke, Garry, et al. "High correlation of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing activity potential and total phenolics content indicates redundancy in use of all three assays to screen for antioxidant activity of extracts of plants from the malaysian rainforest." Antioxidants 2.1 (2013): 1-10.

DPPH (1,1-Diphenyl-2- Picrylhydrazyl ) RADICAL SCAVENGING ACTIVITY

N-N(Ph)2

NO2

NO2 NO2

+ A-H

H-N-N(Ph)2

NO2

NO2 NO2

+ A

DPPH.

DPPH

.

The radical scavenging potential of the test samples are determined by measuring the decrease in absorbance due to the reduced form of DPPH. The absorbance measured at 517nm.

The percentage (%) of Scavenging activity (scv) was calculated from the following equation: % scv = {( A0 – A1 )/A0} X 100 Where, A0 is the absorbance of the control and A1 is the absorbance of the extract/standard.Ref :Clarke, Garry, et al. "High correlation of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing activity potential and total phenolics

content indicates redundancy in use of all three assays to screen for antioxidant activity of extracts of plants from the malaysian rainforest." Antioxidants 2.1 (2013): 1-10.

Fig. 01: % scavenging activity of ascorbic acid and methanol extract of Tetracera Sarmentosa at different concentration

Tetracera Sarmentosa

Ref :Clarke, Garry, et al. "High correlation of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing activity potential and total phenolics content indicates redundancy in use of all three assays to screen for antioxidant activity of extracts of plants from the malaysian rainforest." Antioxidants 2.1 (2013): 1-10.

Table 2: IC50 values of Tetracera Sarmentosa & ascorbic acid (standard)

Sample/Standard IC50 (g/ml)Tetracera Sarmentosa 26.30

Ascorbic acid 1.748

Reducing Power CapacityTable 3: Absorbance of Tetracera Sarmentosa and ascorbic acid (Standard) at four concentrations

Concentration µg/ml

Reducing power of Tetracera Sarmentosa

Reducing power of Ascorbic Acid

125 0.294 1.000

250 0.336 1.504

500 0.458 1.598

1000 0.575 1.878

Ref: Liu, Jun, et al. "Free radical mediated grafting of chitosan with caffeic and ferulic acids: Structures and antioxidant activity." International journal of biological macromolecules 65 (2014): 97-106.

Ref: Carlsen, Monica H., et al. "The total antioxidant content of more than 3100 foods, beverages, spices, herbs and supplements used worldwide.“ Nutrition journal 9.1 (2010): 1.

Table 4:Total phenol content of T.Sarmentosa with necessary data

Sample solution(µg/ml)

Weight of dry extract per mlm(gm)

Absorbance GAE conc.C(µg/ml)

GAE conc.C(mg/ml)

TPC as GAE,A=(µg/ml)

Mean± SEM

1000 0.001 1.04 335.67 0.34 167.83 167.44

1000 0.001 1.034 333.67 0.33 166.83 ±

1000 0.001 1.039 335.33 0.34 167.67 0.31


Antioxidant activity of Dillenia indica L & Pentagyna Roxb

Reducing Power Capability

Concentration µg/ml

Reducing power of Pentagyna Roxb

Reducing powerOf Dillenia indica L.

Reducing power of Ascorbic Acid

0 0 0 0

125 0.979 0.769 1.000

150 1.123 0.869 1.504

500 1.347 1.124 1.598

1000 1.507 1.369 1.878


Antioxidant activity of Dillenia indica L & Pentagyna Roxb

0 200 400 600 800 1000 12000

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6f(x) = 0.00108394376899696 x + 0.606799962006079R² = 0.554314650473266f(x) = 0.00104169452887538 x + 0.461798442249241R² = 0.664866183291377

Reducing potential of Dillenia indica L.& Pentagyna Roxb.

Reducing power of D.ILinear (Reducing power of D.I)Reducing power of D.PLinear (Reducing power of D.P)

Concentration ug/mL

Red

ucin

g po

wer


Determination of total Phenol Content of D.I & P.R

0 100 200 300 400 500 6000

0.5

1

1.5

2

2.5

f(x) = 0.00393934328358209 x + 0.033034825870647R² = 0.998669748560243

Calibration curve of gallic Acid

absorbanceLinear (absorbance)

concentration

Abso

rban

ce





GAE conc.C(mg/ml)


Mean± SEM

1000 0.001 1.04 335.67 0.34 167.83 167.44

1000 0.001 1.034 333.67 0.33 166.83 ±

1000 0.001 1.039 335.33 0.34 167.67 0.31




GAE conc.C(mg/ml)


Mean± SEM

1000 0.001 0.851 252.09 0.27 136.33 133.01

1000 0.001 0.825 264.00 0.26 132.00 ±

1000 0.001 0.837 268.00 0.27 134.00 1.25

Data for the determination phenol content of Dillenia indica L extract

Data for the determination phenol content of Pentagyna Roxb extract


CONCLUSION

Different plants of Dillenicea family showed moderate antioxidant activity by inhibiting DPPH, and reducing power activities when compared with standard ascorbic acid. In addition, the extracts were found to contain a noticeable amount of total phenols and flavonoid which play a major role in controlling oxidation. The results of this study show that the extract can be used as an easily accessible source of natural antioxidant. Therefore, it is suggested that activity–guided isolation study should be performed.

Thank you

A Comprehensive Review Study Of Antioxidant Potential of Dillenicea Family

Health & Medicine

Transcript of A Comprehensive Review Study Of Antioxidant Potential of Dillenicea Family