A cancer drug exploration
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Transcript of A cancer drug exploration
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Secrets of the Rainforest
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The Mysterious Green Florescent Leaves
While Hiking through the Andean Rain Forest, You and a friend, Tisha, obtain medicinal green leaves from Ramon
You bring back to Biotex, a biotech company working on a cure for cancer
Hi, I am Tish
a
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Green Florescent Protein
Biotex scientists determine that the leaves contain a florescent green protein
DNA from the leaves is inserted into bacterial cells
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Cloning the Gene from Mysterious Leaves
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More on Cloning the Gene…
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Purifying the GFP
Biotex technicians need to separate the GFP from other bacterial proteins
Column Chromatography separates contaminating proteins from GFP
The result is a purified GFP that can be tested for its ability to cure stomach cancer
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Testing and Placebos
GFP will need to be tested on rats before it is allowed for a human trial
The US Food and Drug Administration will examine Biotex’s experiments to determine if the protein is safe and cures stomach cancer without negative side effects
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Ethics and Economics
Once GFP is approved by the FDA, you will develop GFP as a commercial product
You will need to be aware of the following viewpoints: FDA, social conscience, patient advocacy groups, and the employees of Biotex
You must resolve the dilemmas in order to successfully market GFP
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Final Presentations
Turn in all lab work with analysis questions
Video Presentation of your marketing of GFP Address issues of all
dilemmas 1-8
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Background Information- Lesson 1
How to use Pipettes
Streaking of agar plates to Produce Single Colonies (Sterile Technique)
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Background Continued…Lessons 2 & 3
Process of Transformation (GMOs or genetic recombinants)
Jellyfish , GFP and pGLO and Fluorescence
Isolating Clones to Propagate in Liquid Culture Media
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Background Continued…Lesson 4
Concentration of Bacteria from Liquid Culture Media
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Background Continued…Lesson 5
Prepare a Bacterial Lysate for Protein Chromatography
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Background Continued…Lesson 6
Protein Chromatography-Hydrophobic Interaction Chromatography (HIC)
GFP is hydrophobic and will stick to hydrophobic beads in salt water buffer
When salt is removed, GFP will then be collected
4 buffers: 1) Equilibration
buffer (salt buffer) 2) Binding Buffer
(high salt) Wash buffer (medium
salt) Elution buffer (low
salt)
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Background Continued…Lessons 7-10
Lesson 7- testing and placebos Lesson 8- Advocacy Groups and
Viewpoints Lesson 9-Ethics and Economics Lesson 10- Preparing and Giving
Presentations