8.10.2012 Jonathan Keytel€¦ · Custom Biotech and bioreagents. 3 Roche Applied Science Solutions...
Transcript of 8.10.2012 Jonathan Keytel€¦ · Custom Biotech and bioreagents. 3 Roche Applied Science Solutions...
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Roche Applied Science Solutions
H3 Africa Meeting
8.10.2012
Jonathan Keytel
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Overview Roche Diagnostics
in vitro Diagnostics Life Science
Professional
Diagnostics
Business Area
Molecular
Diagnostics
Business Area
Tissue
Diagnostics
Business Area
Diabetes Care
Business Unit
Applied Science
Business Area
• Serum work area/
immunoassays and
clinical chemistry
• Point-of-care testing
• Coagulation and
specialty testing
• Workflow management
• Virology
• Blood screening
• Genomics/oncology
• Microbiology
• Women’s health
• Primary staining
• Advanced staining
• Workflow management
• Digital pathology
• Blood glucose
monitoring
• Insulin delivery
• Diabetes management
systems
Nucleic acid DNA
purification and
gene expression
• Sequencing solutions
• Custom Biotech and
bioreagents
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Roche Applied Science
Solutions for scientific research across three areas
Genomic
Research Cell
Analysis
Genome Sequencing
Innovatio
n
https://www.roche-applied-science.com
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Supporting Africa from Africa Roche Support on the continent
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Committed to Sustainable Development in Africa
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SPECIFIC SOLUTIONS
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Sample Handling and NA Extraction Solution
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Aliqoting
Storage
Purification
RNA/DNA
Primary
Sample
Handling
MagNA Pure 96
Hamilton PSU STARlet
Appropriate
Freezer System
Hamilton MagNA STARlet
Automated Sample Workflow
Automated Data Workflow
-80 Freezers or
Liquid Nitrogen
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Targeted Re-Sequencing with Sequence Capture
Get only your relevant parts from any genome
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NimbleGen SeqCap EZ Libraries
Capture any target from <100Kb to 100Mb and beyond
• 2.1 Million Long DNA (robust) oligos
– Highest number for high coverage, great uniformity
• Easy, Solution based, error-proof
– qPCR controls, multiplex capable (pre or post)
• Best performing capture
– Discovers more mutations than competitors
More probes – better coverage
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SeqCap EZ Discovery Research Products
Comprehensive Exome and UTR designs
Products Design Size
SeqCap EZ Exome v2.0 44Mb
SeqCap EZ Exome v3.0 64Mb
SeqCap EZ Exome +UTR 96Mb
SeqCap EZ Exome Plus Up to 114Mb
SeqCap EZ UTR only 50Mb
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Vector
preparation Insert
preparation
Transformatio
n & Screening
Protein expression
& purification
Ligation
Primer Probe
Design Nucleic Acid
Purification
cDNA
Synthesis
Sample
preparation
Sample
preparation
Primer Probe
Design
Integrated Genomic Research Solutions
Nucleic Acid
Purification
RT-PCR
PCR
Working with RNA
Working with DNA
Cloning and protein expression
https://www.roche-applied-science.com
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Sequencing
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CONTACTS
Jonathan Keytel
Marketing Manager Roche Applied Science
Office: +27 11 504 4675
Mobile: +27 82 827 3333
Website: www.roche-applied-science.com
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