72 mice total (72 brain RNA samples + 48 bone RNA samples)
5
B6 B6 F F M M E E C C E E D2 D2 F F M M C C E E 9 9 9 72 mice total (72 brain RNA samples + 48 bone RNA samples) Exercise Project Matrix 9 9 9 9 9 B B6 strain D D2 strain M male F female E exerci se C contro l Key: Collaboration with Dr. Sharkey
description
Collaboration with Dr. Sharkey. Exercise Project Matrix. Key:. B6. D2. M. F. M. F. C. E. C. E. E. 9. 9. 9. 9. 9. 9. 9. 9. 72 mice total (72 brain RNA samples + 48 bone RNA samples). Brain RNA QC – quality (260/280) & quantity (ug). Key:. - PowerPoint PPT Presentation
Transcript of 72 mice total (72 brain RNA samples + 48 bone RNA samples)
B6B6
FFMM
EE CC EE
D2D2
FFMM
CC EE
9 9 9
72 mice total (72 brain RNA samples + 48 bone RNA samples)
Exercise Project Matrix
9 9 9 9 9
B B6 strain
D D2 strain
M male
F female
E exercise
C control
Key:Collaboration with Dr. Sharkey
April 2005 Brain Dissections Total = 23 samples
Sample # Sample type 260/280 [ ] ug/ul ul used for spec ul used for gel ul left from 50ul ~ ug
1 BME 1.8 0.11 10 9 31 3
2 BME 1.8 0.12 10 9 31 4
3 BME 1.8 0.13 10 8 32 4
4 BMC 1.8 0.10 10 10 30 3
5 BMC 1.8 0.12 10 9 31 4
6 BMC 1.9 0.09 10 11 29 3
7 BFE 1.8 0.14 10 7 33 4
8 BFE 1.8 0.07 10 14 26 2
9 BFE 2 0.09 15 4 31 3
10 BFC 1.8 0.14 10 7 33 5
11 BFC 1.8 0.14 10 7 33 5
13 DME 1.8 0.07 10 14 26 2
14 DME 1.9 0.09 10 12 28 2
15 DME 1.8 0.13 10 7 33 3
16 DMC 1.7 0.10 10 10 30 4
17 DMC 1.8 0.13 10 8 32 3
18 DMC 1.9 0.12 10 8 32 4
19 DFE 1.8 0.09 10 11 29 4
20 DFE 1.9 0.09 10 11 29 3
21 DFE 1.9 0.12 10 9 31 3
22 DFC 2 0.07 10 15 25 3
23 DFC 1.9 0.10 10 10 30 2
24 DFC 1.9 0.1 10 8 32 3
B B6 strain
D D2 strain
M male
F female
E exercise
C control
Key:
Brain RNA QC – quality (260/280) & quantity (ug)
April 2005 Bone Dissections Total = 16 samples
Sample # Sample type 260/280 [ ] ug/ul ul used for spec ul used for gel ul left from 50ul ~ ug
1 BME 1.8 0.0904 10 11 29 3
3 BME 1.7 0.0264 10 0 40 1
4 BMC 1.8 0.0848 10 12 28 2
6 BMC 1.7 0.0288 10 0 40 1
7 BFE 1.8 0.12 10 8 32 4
9 BFE 1.6 0.0308 10 0 40 1
10 BFC 1.7 0.0364 10 0 40 1
11 BFC 1.7 0.042 10 0 40 2
13 DME 1.7 0.0332 10 0 40 1
15 DME 1.7 0.0188 10 0 40 1
16 DMC 1.8 0.068 10 0 40 3
17 DMC 1.6 0.0736 10 0 40 3
19 DFE 1.5 0.0424 10 0 40 2
21 DFE 1.6 0.0552 10 0 40 2
22 DFC 1.7 0.058 25 0 25 1
24 DFC 1.3 0.0208 10 0 40 1
B B6 strain
D D2 strain
M male
F female
E exercise
C control
Key:
Bone RNA QC – quality (260/280) & quantity (ug)
Bone SamplesBrain Samples
Bone & Brain RNA QC – quality
Feedback
• Further RNA QC (bone samples gel & bioanalyzer)
• Pool samples?
• Replicates? (# of chips & RT-PCR)
