6 introduction fruit breedomics mab services laurence francois
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Transcript of 6 introduction fruit breedomics mab services laurence francois
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FruitBreedomics Molecular Assisted Breeding transfer
Pere Arus, Joan Bonany, François Laurens
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Reminder: some results of apple and peach breeding questionnaires (2011-2012)
Total answers:- Apple: 31- Peach: 18
Fundings Time/labour
space Club variety pollen
availability
Available germplasm novel traits
10 5 6 1 1
Tests for resistance
assessment
Tests for quality
assessment
Widening genetic basis for resistance
Markers for resistance
genes
Climatic factors
2 1 2 1 1
Current Bottlenecks in apple breeding programmes
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Reminder: some results of apple and peach breeding questionnaires (2011-2012)
Are molecular markers being used currently in your breeding program?
Yes:Apple: 43% (on 21)Peach: 31% (on 12)
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Apple peach
Parent selection 8 3
Hybrid selection 6 5Identification of hybrids or varieties 5 4
Identification of parentage 5 5
Genetic profile for intellectual property defense
2 3
What are molecular markers used for in your breeding program?
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No added value6%
No training in usage of molecular
markers for breeding purposes
11%
No technology available
22%
No markers available for traits
of interest22%
Non economically viable39%
What are the reasons for not using molecular markers in the breeding program ?
No training in usage of molecu-lar markers for breeding pur-poses 33.3%
No technology available ; 22.2%
No markers available for traits of interest 22.2%
Non econom-ically viable
44.4%
What are the main reasons for not using molecular markers in the breeding programs?
Apple
Peach
Þ Aim of FruitBreedomics = tackle these technical,
economical, training issues
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YOUR LOGO
What FruitBreedomics provided so far and will provide?
Big genotyping SNP chips: peach IRSC=8K; apple: 20K, 420K
=> Fine mapping => find out markers close to the traits /Apple and
peach–Major genes –QTLs=> From various genetic backgrounds (PBA, GWA)
Test high throughput and cheap genotyping tools adapted to Breeders needs
MAB Pilot studies
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YOUR LOGO
FruitBreedomics MAB ServicesProposal
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YOUR LOGO
Major challenges faced by fruit breeders on the use of MAB
Fruit Breeding companies = small size companies=> limited access to expertise in genetics, genomics, marker technologies,... => Lack of lab facilities for molecular analysis, bioinformatics support,...
FruitBreedomics needs to:- address the specific problems of each company concerning the use of markers in their breeding programs- preserve confidentiality on parents, breeding strategies, key characters, etc.
FruitBreedomics planned to provide breeders with support on MAB - Pilot study
- Training sessions => A more individualized support was needed
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Transfer technology to stakeholders
….
GENOTYPINGPLATFORM
KNOWLEDGE PROVIDERS(PARTNERS)
FRUITBREEDOMICSMAB
SERVICES
Advice on potential useOn site visitsOn site assistanceSamplingSupport decisionData analysis Companies with experience on MAB transfer
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YOUR LOGO
What FruitBreedomics MAB Service should be
• A service offered on a commercial basis • Offered to FruitBreedomics stakeholder
breeding programs in Rosaceae species• Using FruitBreedomics (and other published)
generated information • Not a genotyping Platform
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Transfer technology to stakeholders
Végépolys
/INRAFEM IRTA
….
GENOTYPINGPLATFORM
KNOWLEDGE PROVIDERS(PARTNERS)
FRUITBREEDOMICSMAB
SERVICES
2014 >2015
Implementation