6 introduction fruit breedomics mab services laurence francois

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FruitBreedomics Molecular Assisted Breeding transfer Pere Arus, Joan Bonany, François Laurens

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Transcript of 6 introduction fruit breedomics mab services laurence francois

Page 1: 6 introduction fruit breedomics mab services laurence francois

FruitBreedomics Molecular Assisted Breeding transfer

Pere Arus, Joan Bonany, François Laurens

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Reminder: some results of apple and peach breeding questionnaires (2011-2012)

Total answers:- Apple: 31- Peach: 18

Fundings Time/labour

space Club variety pollen

availability

Available germplasm novel traits

10 5 6 1 1

Tests for resistance

assessment

Tests for quality

assessment

Widening genetic basis for resistance

Markers for resistance

genes

Climatic factors

2 1 2 1 1

Current Bottlenecks in apple breeding programmes

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Reminder: some results of apple and peach breeding questionnaires (2011-2012)

Are molecular markers being used currently in your breeding program?

Yes:Apple: 43% (on 21)Peach: 31% (on 12)

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Apple peach

Parent selection 8 3

Hybrid selection 6 5Identification of hybrids or varieties 5 4

Identification of parentage 5 5

Genetic profile for intellectual property defense

2 3

What are molecular markers used for in your breeding program?

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No added value6%

No training in usage of molecular

markers for breeding purposes

11%

No technology available

22%

No markers available for traits

of interest22%

Non economically viable39%

What are the reasons for not using molecular markers in the breeding program ?

No training in usage of molecu-lar markers for breeding pur-poses 33.3%

No technology available ; 22.2%

No markers available for traits of interest 22.2%

Non econom-ically viable

44.4%

What are the main reasons for not using molecular markers in the breeding programs?

Apple

Peach

Þ Aim of FruitBreedomics = tackle these technical,

economical, training issues

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YOUR LOGO

What FruitBreedomics provided so far and will provide?

Big genotyping SNP chips: peach IRSC=8K; apple: 20K, 420K

=> Fine mapping => find out markers close to the traits /Apple and

peach–Major genes –QTLs=> From various genetic backgrounds (PBA, GWA)

Test high throughput and cheap genotyping tools adapted to Breeders needs

MAB Pilot studies

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YOUR LOGO

FruitBreedomics MAB ServicesProposal

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YOUR LOGO

Major challenges faced by fruit breeders on the use of MAB

Fruit Breeding companies = small size companies=> limited access to expertise in genetics, genomics, marker technologies,... => Lack of lab facilities for molecular analysis, bioinformatics support,...

FruitBreedomics needs to:- address the specific problems of each company concerning the use of markers in their breeding programs- preserve confidentiality on parents, breeding strategies, key characters, etc.

FruitBreedomics planned to provide breeders with support on MAB - Pilot study

- Training sessions => A more individualized support was needed

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Transfer technology to stakeholders

….

GENOTYPINGPLATFORM

KNOWLEDGE PROVIDERS(PARTNERS)

FRUITBREEDOMICSMAB

SERVICES

Advice on potential useOn site visitsOn site assistanceSamplingSupport decisionData analysis Companies with experience on MAB transfer

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YOUR LOGO

What FruitBreedomics MAB Service should be

• A service offered on a commercial basis • Offered to FruitBreedomics stakeholder

breeding programs in Rosaceae species• Using FruitBreedomics (and other published)

generated information • Not a genotyping Platform

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Transfer technology to stakeholders

Végépolys

/INRAFEM IRTA

….

GENOTYPINGPLATFORM

KNOWLEDGE PROVIDERS(PARTNERS)

FRUITBREEDOMICSMAB

SERVICES

2014 >2015

Implementation