5990-7705CHTW

7/25/2019 5990-7705CHTW

1/4

Poroshell 120 1290

Minjia HUANG

Agilent Technologies, Inc.

100102 3

Rongjie FU

200131 412

Poroshell 120 EC-C18 1290 (LC)

(azo colorant) 22 (aromatic amine)

4 14 26 (aniline)

-N=N-

(EU)

EN14362 [1, 2] 1 22

141314

3.0 150mm2.7 m Poroshell 120 EC-C18

1290 22 4 (1,

4-phenylendiamineaniline2,6-dimethylaniline2,4-dimethylaniline) EN 14362

7/25/2019 5990-7705CHTW

2/4

1. 141314

(CAS)

1 1,4-phenylendiamine 106-50-3

2 4-methoxy-m-phenylenediamine 615-05-4

3 4-methyl-m-phenylenediamine 95-80-7

4 (Aniline) 62-53-3

5 benzidine 92-87-5

6 o-anisidine

2-methoxyaniline 90-04-0

7 4,4'-oxydianiline 101-80-4

8 o-toluidine

2-aminotoluene 95-53-4

9 4-chloroaniline 106-47-8

10 5-nitro-o-toluidine 99-55-8

11 4,4'-methylenedianiline

4,4'-diaminodiphenylmethane 101-77-9

12 6-methoxy-m-toluidine

p-cresidine 120-71-8

13 2,6-dimethylaniline 87-62-7

14 2,4-dimethylaniline 95-68-1

15 3,3'-dimethoxybenzidine

o-dianisidine 119-90-4

16 3,3'-dimethylbenzidine

4,4'-bi-o-toluidine 119-93-7

17 4,4'-thiodianiline 139-65-1

18 2-naphthylamine 91-59-8

19 4-chloro-o-toluidine 95-69-2

20 2,4,5-trimethylaniline 137-17-7

21 4,4'-methylenedi-o-toluidine 838-88-0

22 biphenyl-4-ylamine

4-aminobiphenylxenylamine 92-67-1

23 3,3'-dichlorobenzidine3,3'-dichlorobiphenyl-4,4'-ylenediamine 91-94-1

24 4-aminoazobenzene 60-09-3

25 4,4'-methylene-bis-(2-chloro-aniline)

2,2'-dichloro-4.4-methylene-dianiline 101-14-4

26 o-aminoazotoluene

4-amino-2',3-dimethylazobenzene

4-o-tolylazo-o-toluidine 97-56-3

2

1290 LC

(TCC)

(DAD)

3.0 150 mm2.7 m Poroshell 120 EC-

C18 (p/n: 693975-302)

1 26 J&K

A (10:90)

12 g/mL 4 C

HPLC

Poroshell 120 EC-C183.0 mm 150 mm2.7 m

(p/n: 693975-302)

40 C

0.8 mL

A0.575 g (Mono ammonium phosphate)

0.7 g (Disodium hydrogen phosphate)

900 mL 100 mL pH

pH 6.9

B

DAD 240 nm280 nm305 nm

1 L

4.6 250 mm5 m

70 [1] Poroshell 120 SB-C18 EC-C18

Poroshell 120 EC-C18

2.7 m

2m

150 mm

4, 4'-methylenedianiline

116-methoxy-m-toluidine (12)2,6-dimethylaniline

(13)2,4-dimethylaniline (14)3,3'-dimethoxybenzidine (15)

7/25/2019 5990-7705CHTW

3/4

3,3'-dimethylbenzidine (16)

2

126

15

80%

3.0 mm 4.6 mm

60%

(UV)

240 nm280 nm305 nm

2. 26

B%

0 14

4.5 29

8 29

9 50

10.5 65

12 90

14 100

15 14

2.7 m 1.8 m

40% 150 mm

580 bar 600 bar UHPLC

Poroshell 120 EC-C18

3.0 mm 150 mm2.7 m 1290

2 2

3

0 2 4 6 8 10 12

mAU

-10

0

10

20

30

40

1

2

3

4

5

6

7

8

9

10 11

1213

14

1516

17

18

1920

21

22

23

24

26

25

1. 3.0 mm 150 mm2.7 mPoroshell 120 EC-C18

240 nm 26

0 2 4 6 8 10 12

0 2 4 6 8 10 12

0 2 4 6 8 10 12

mAU

0

20

40

mAU

0

20

40

mAU

0

20

40

1 2

3

4

56

7

8

9

10 111213

141516

17

18

19 20

21

22 23

2426

25

26

26

20 21

2223

24

25

22

23

2425

1817111213

14

1516

910

910

1516

5

6 7 8

7

5

4

240 nm

280 nm

305 nm

2. 240 nm280 nm305 nm

7/25/2019 5990-7705CHTW

4/4

www.agilent.com/chem

2011

2011 4 12

5990-7705CHTW

3.0mm

150mm2.7 um Poroshell 120 EC-C18

15 26

1290

80%

60%

1. EN 14362-1-2003 -

1 (Textiles -

Methods for determination of certain aromatic amines

derived from azo colorants.Part 1:Detection of the use of

certain azo colorants accessible without extraction.)

2. EN 14362-2-2003 -

2

(Textiles.Methods for the determination of certain

aromatic amines derived from azo colorants.Part

2:Detection of the use of certain azo colorants

accessible by extracting the fibres.)

0 2 4 6 8 10 12

-10

0

10

20

30

40

DAD1 A, Sig=240,4 Ref=off (AZOS DYES\...DYES-POROSHELL 120EC\DEF_LC 2010-08-1909-56-21\20100819000001.D)





DAD1 A, Sig=240,4 Ref=off (AZOS DYES\AZODYE-POROSHELLEC\20100819000002.D)

3. 6 240 nm

5990-7705CHTW

Documents

Transcript of 5990-7705CHTW