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Figure S4. Time lapse movie showing polarized TIRF of 1:1:1 DOPC:DSPC:cholesterol supported bilayers labelled with the fluorescent probe DiI-C 18 . 30 um x 30um, 20 min, 20 fps/ Images were taken for 20 min at 20 fps for parallel excitation (Fs) and perpendicular excitation (Fp) of the fluorescent probe following the addition of PrP(106- 126) oligomers to a final concentration of 4.1µM. Rapid switching of the polarization facilitated acquisition of near-simultaneous images for each polarization. The intensely bright dots in each panel are due to the presence of a vesicle within the pTIRF illumination field. The third panel shows the order parameter <P2> at each pixel, calculated for each frame as described in the text. Each panel represents the same 30 m x 30 m area of the bilayer, and is an expansion of the 15 m x 15 m area shown in Figure 6B. F S F P <P 2 >

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F S. F P. . 5 µm. - PowerPoint PPT Presentation

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Figure S4. Time lapse movie showing polarized TIRF of 1:1:1 DOPC:DSPC:cholesterol supported bilayers labelled with the fluorescent probe DiI-C18. 30 um x 30um, 20 min, 20 fps/ Images were taken for 20 min at 20 fps for parallel excitation (Fs) and perpendicular excitation (Fp) of the fluorescent probe following the addition of PrP(106-126) oligomers to a final concentration of 4.1M. Rapid switching of the polarization facilitated acquisition of near-simultaneous images for each polarization. The intensely bright dots in each panel are due to the presence of a vesicle within the pTIRF illumination field. The third panel shows the order parameter at each pixel, calculated for each frame as described in the text. Each panel represents the same 30 m x 30 m area of the bilayer, and is an expansion of the 15 m x 15 m area shown in Figure 6B.FSFP

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