4.8 To prepare and show the production of alcohol by...
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![Page 1: 4.8 To prepare and show the production of alcohol by yeastmccscience.yolasite.com/resources/4.8AlcoholbyYeastV.pdf · 4.8 To prepare and show the production of alcohol by yeast 1](https://reader031.fdocuments.in/reader031/viewer/2022022505/5ab8fb157f8b9ad3038d74c0/html5/thumbnails/1.jpg)
4.8 To prepare and show the production of alcohol by yeast
1
Add 200 ml glucose solution to each of two conical flasks.
2
Add 5 g of yeast to one flask. The second flask contains no yeast – this acts as a control.
3
Label the flasks:‘Yeast + Glucose’ and ‘Control’
4
Half fill the fermentation locks with water and put one on each flask.
5
Stand the flasks in a water bath at 30OC for 24 hours.
6
Result: Fermentation is complete when the bubbles stop.
Always remember – Leave time to tidy up This page can be printed in colour from the accompanying DVD
![Page 2: 4.8 To prepare and show the production of alcohol by yeastmccscience.yolasite.com/resources/4.8AlcoholbyYeastV.pdf · 4.8 To prepare and show the production of alcohol by yeast 1](https://reader031.fdocuments.in/reader031/viewer/2022022505/5ab8fb157f8b9ad3038d74c0/html5/thumbnails/2.jpg)
7
Filter the contents of each flask into separate boiling tubes.
8 9
Add 5 ml sodium hypochlorite to eachboiling tube.
10
Place boiling tubes in a water bath at 60OC for 4-5 minutes.
11
Expected result
Always remember – Leave time to tidy up This page can be printed in colour from the accompanying DVD
Add 3 ml potassium iodide to each boiling tube.
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Results Flask Original colour of Final colour of Other changes filtrate filtrate Yeast + Glucose solution Control (no yeast)
Always remember – Leave time to tidy up This page can be printed in colour from the accompanying DVD