4.3 (b) To investigate the effect of pH on catalase activity.
-
Upload
kristian-george -
Category
Documents
-
view
215 -
download
0
Transcript of 4.3 (b) To investigate the effect of pH on catalase activity.
![Page 1: 4.3 (b) To investigate the effect of pH on catalase activity.](https://reader035.fdocuments.in/reader035/viewer/2022062423/5697bfd81a28abf838caeebc/html5/thumbnails/1.jpg)
4.3 (b) To investigate the effect of pH on catalase activity
![Page 2: 4.3 (b) To investigate the effect of pH on catalase activity.](https://reader035.fdocuments.in/reader035/viewer/2022062423/5697bfd81a28abf838caeebc/html5/thumbnails/2.jpg)
Before you start get or make a copy of this
Table of results
pH of bufferInitial volume
(ml)Final volume
(ml)Volume of foam produced (ml)
![Page 3: 4.3 (b) To investigate the effect of pH on catalase activity.](https://reader035.fdocuments.in/reader035/viewer/2022062423/5697bfd81a28abf838caeebc/html5/thumbnails/3.jpg)
Step 1
Finely chop the celery.
Weigh 5 g of the chopped celery.
![Page 4: 4.3 (b) To investigate the effect of pH on catalase activity.](https://reader035.fdocuments.in/reader035/viewer/2022062423/5697bfd81a28abf838caeebc/html5/thumbnails/4.jpg)
Step 2
Add 20 ml of buffer pH 4, one drop of washing-up liquid and 5 g of
celery into a graduated cylinder.
![Page 5: 4.3 (b) To investigate the effect of pH on catalase activity.](https://reader035.fdocuments.in/reader035/viewer/2022062423/5697bfd81a28abf838caeebc/html5/thumbnails/5.jpg)
Step 3
Add 2 ml of hydrogen peroxide to a boiling tube.
![Page 6: 4.3 (b) To investigate the effect of pH on catalase activity.](https://reader035.fdocuments.in/reader035/viewer/2022062423/5697bfd81a28abf838caeebc/html5/thumbnails/6.jpg)
Step 4
Place the graduated cylinder and the boiling tube in a water bath at 25 °C.
![Page 7: 4.3 (b) To investigate the effect of pH on catalase activity.](https://reader035.fdocuments.in/reader035/viewer/2022062423/5697bfd81a28abf838caeebc/html5/thumbnails/7.jpg)
Step 5
Add the hydrogen peroxide into the graduated cylinder and
record the volume immediately.
![Page 8: 4.3 (b) To investigate the effect of pH on catalase activity.](https://reader035.fdocuments.in/reader035/viewer/2022062423/5697bfd81a28abf838caeebc/html5/thumbnails/8.jpg)
Step 6
Time for 2 minutes and record the final volume.
![Page 9: 4.3 (b) To investigate the effect of pH on catalase activity.](https://reader035.fdocuments.in/reader035/viewer/2022062423/5697bfd81a28abf838caeebc/html5/thumbnails/9.jpg)
Step 7
Repeat the steps for
different pH buffer solutions
e.g. 6, 7, 9, 10.
![Page 10: 4.3 (b) To investigate the effect of pH on catalase activity.](https://reader035.fdocuments.in/reader035/viewer/2022062423/5697bfd81a28abf838caeebc/html5/thumbnails/10.jpg)
Table of results:
pH of bufferInitial volume
(ml)Final volume
(ml)Volume of foam produced (ml)
![Page 11: 4.3 (b) To investigate the effect of pH on catalase activity.](https://reader035.fdocuments.in/reader035/viewer/2022062423/5697bfd81a28abf838caeebc/html5/thumbnails/11.jpg)
Expected Graph
![Page 12: 4.3 (b) To investigate the effect of pH on catalase activity.](https://reader035.fdocuments.in/reader035/viewer/2022062423/5697bfd81a28abf838caeebc/html5/thumbnails/12.jpg)
END