4 Pradier Metarhizium IBMA Luzern 2008 - ABIM -Home · Simplified Life Cycle of Metarhizium...

22/10/2008

Met52Bio-insecticide

Metarhizium anisopliae Strain F52

Thierry Pradier

Novozymes Biologicals

NOVOZYMES PRESENTATION22/10/20082

Overview

Brief M. anisopliae Cycle

Product Description

Efficacy and Related Data

Major targets

Secondary targets

Summary


Simplified Life Cycle of Metarhizium anisopliae

Saprophyte in Soil

Infection throughthe Cuticle

Mortality and Sporulation

Horizontal Transmission


Product Description

2 formulations

Granular Formulation on rice for soil applications

EC Formulation for spray


Metarhizium has demonstrated some control on:• Black Vine Weevil• Blacklegged Ticks• Greenhouse Whitefly• Green Peach Aphid• Two Spotted Spider Mite• Western Flower Thrip• Root Maggots (cabbage, sugarbeet)• Cotton Aphid• Japanese Beetle

Met52tm Bioinsecticide : Spectrum of activity


1st Major Targets

Denny Bruck, USDA-ARS

Denny Bruck, USDA-ARS Kirby Stafford, CT Ag. Expt. Station

Kirby Staffor, CT Ag. Expt. Station

Black Vine Weevil Ticks


Worldwide Distribution of Black Vine Weevil

150+ Host Plant Species


Competitive Efficacy with Chemical Standards(Black Vine Weevil)

Control Met52 G1g/L

Met52 G2g/L

Met52 G5g/L

Bifenthrine40 oz/100gal

Bendiocarb4 oz/

100 gal

Imidacloprid32 g/

100gal

0

5

10

15

20

Liv

e L

arv

ae /

Po

t (3

wk P

ost

-Trt

)

Dan Gilrein, 1999, Cornell Cooperative Extension


Persistent Control of Black Vine Weevil in Potting Media

5

5.5

6

6.5

7

7.5

0 50 100 150 200 250 300 350Days Post Fungus Application

Log 1

0C

FU/g

of P

ottin

g M

edia

Bruck (2005) Biological Control 32:155-163

97.5% Infection ofLast Instar BVW


Temperature Optima of M. anisopliae F52

Moorhouse et al. (1994) Ann Appl. Biol. 124:185-193

0

5

10

15

20

25

30

35

10° C(50° F)

15° C(59° F)

20° C(68° F)

25° C(77° F)

LT50

(Day

s) B

VW L

arva

e


Fungicide Compatibility of M. anisopliae F52

0

10

20

30

40

50

60

70

80

90

100

Met

52

Ben

omyl

+Met

52

Bup

irim

ate+

Met

52

Car

bend

azim

+Met

52

Chl

orot

halo

nil+

Met

52

Etrid

iazo

le+M

et52

Fena

rimol

+Met

52

Fura

laxy

l+M

et52

Ipro

dion

e+M

et52

Prop

amoc

arb+

Met

52

Pyra

zaph

os+M

et52

Tolc

lofo

s-m

ethy

l+M

et52

Zine

b+M

et52

Moorhouse et al. (1992) Biocontrol Sci. Technol. 2:49-58

% C

ontr

ol o

f Bla

ck V

ine

Wee

vil


1st Major Targets

Denny Bruck, USDA-ARS

Denny Bruck, USDA-ARS Kirby Stafford, CT Ag. Expt. Station

Kirby Staffor, CT Ag. Expt. Station

Black Vine Weevil Ticks


Worldwide Distribution of Lyme Disease


Integration of M. anisopliae into Tick IPM

Stafford (2004) Tick Control Handbook. CT. Ag. Expt. Station Publication


Control of Black Legged Ticks with Tick-EX EC New Jersey Field Trial

Days Post Treatment

Ave

rage

Tic

ks p

er D

rag

0

10

20

30

40

50

60

70

80

0 7 14 21 28 35 42 49 56 63 70 77 84

Control-NymphTick EX EC-NymphControl-LarvaeTick EX EC-Larvae

Single Application at 1X


M. anisopliae F52 Persistence in Tick Environments New Jersey Field Trial

0.00E+00

5.00E+04

1.00E+05

1.50E+05

2.00E+05

2.50E+05

3.00E+05

3.50E+05

Jun 5 Jun 12 Jun 19 Jun 26 Jul 3 Jul 10

Date

CFU

's/g

of le

af li

tter

Treated

Control


Greenhouse and Field Trials

Whiteflies

Thrips:Wireworms

Grubs:Root Maggots:

Leaf-Feeding Beetles

Cockroaches

Mites

Weevils

Borers

Ticks

Nursery and OrnamentalsLawn and Landscape PCO

ForestrySustainableAgricultureApiculture

Flies


Greenhouse and Field TrialsNursery and Ornamentals• Black vine weevil, Japanese Beetle, Oriental Beetle• Whiteflies, thrips, mites, aphids• Leaf-feeding beetles• Scale, mealybugs

Lawn and Landscape•Ticks•Turf Grubs• Crane flies• Chinch bugs• Billbugs

PCO• Cockroaches• Ants

Forestry• Poplar plantations• Tick control• Asian longhorn beetle

SustainableAgriculture• Vegetables• Berries• Tree Fruits/Nuts• Root crops• Organic

Apiculture• Small hive beetle• Varroa mite

• 90 Field Trials• 40 Insect Targets


Whitefly Control with Met52 EC

0

20

40

60

80

100

120

140

160

180

0 14 28

Days

Tota

l Whi

tefli

es p

er L

eaf

ControlMet52 LowMet52 MidMet52 High


Two-Spotted Spider Mite Control with Met52 EC

0

2

4

6

8

10

12

14

0 14 28Days

Mite

s pe

r Lea

f

ControlM et52 LowM et52 High


Thrip Control and Onion Yield from Met52 EC

0

10

20

30

40

50

60

70

80

Thrips/plant Foliar damage % larger onions (#) % larger onions(wt.)

Met52 ECControl


Potential for Thrip Pupae Control in Soil

Ma=Met52Imi=ImidaclopridFip=FipronilFC= Full ConcentrationSLD=Sub-lethal DosePM= PremixedDR=Drench Ansari et al (2007) Biological Control. 40: 293-297


Mole Crickets – Laboratory Bioassays

0102030405060708090

100

0 2 4 6 8

Days

% M

orta

lity

4x10E5 spores/mL 4x10E6 spores/mL4x10E7 spores/mL Untreated Control


Western Corn Rootworm

0102030405060708090

100

Met52ECat 2x104

Met52ECat 2x105

Met52ECat 2x106

Met52G at3x106

Met52G at3x107

Control

Spores per Gram of Soil

% M

orta

lity


Summary

Granular and EC formulations available

Proven efficacy by independent researchers

Residual soil activity (one year in potting media)

Expanding labels for granular and EC to include food use and wider pest spectrum

Registered in USA and Holland

EU registration process currently going-on (on active ingredient forth list): decision for Annex 1 inclusion end 2008 – early 2009

4 Pradier Metarhizium IBMA Luzern 2008 - ABIM -Home · Simplified Life Cycle of Metarhizium...

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