4 Ctkc Bp Vitekms Simplified User Guide en 2012mar08
Transcript of 4 Ctkc Bp Vitekms Simplified User Guide en 2012mar08
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S im p l i f i e d U s e r u i d e
V I T EK MS Sim p l i f ie d U s e r G u id e V e rs i o n 4 p a g e 1
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V I T EK MS Sim p l i f ie d U s e r G u id e V e r si o n 4 p a g e 2
Table of contentsVITEK MS instrument
Configuration...
Workflow
Material and consumables ..
Protocol ID&AST..
Protocol ID
Protocol AST. page 10
page 4
page 5
page 6
page 7
page 8
page 9
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V I T EK MS Sim p l i f ie d U s e r G u id e V e r si o n 4 p a g e 3
VITEK MS Prep Station page 11
MYLA Slides composition.... page 12
VITEK MS Acquisition Station. pages 13-15
MYLA : Results to be reviewed... pages 16-19
MYLA : Results to be approved.. pages 20-21
MYLA : Electronic Signature page 22
MYLA : AST review..... page 23
VITEK MS Users maintenance... page 24
Table of contents
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V I T EK MS Sim p l i f ie d U s e r G u id e V e r si o n 4 p a g e 4
VITEK MS Instrument
Indicates an alarm
Status OK
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V I T EK MS Sim p l i f ie d U s e r G u id e V e r si o n 4 p a g e 5
MS-ID Database
( CE/IVD) embedded into MylaTM
P ow er I on is in g V ac uu m N eg at iv eP ow er I on is in g V ac uu m N eg at iv eP ow er I on is in g V ac uu m N eg at iv e
Po w e r Io n is in g V ac uu m N eg at iv ePo w e r Io n is in g V ac uu m N eg at iv ePo w e r Io n is in g V ac uu m N eg at iv e
Prep Station
Configuration
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V I T EK MS Sim p l i f ie d U s e r G u id e V e r si o n 4 p a g e 6
Workflow
Acquisition Station
P ow er Io ni si ng V ac uu m Neg at iv eP ow er Io ni si ng V ac uu m Neg at iv eP ow er Io ni si ng V ac uu m Neg at iv e
Prep Station
Slidedescription
Spectra
Slidedescription
Peak lists
Slide
description
MS-ID Database
( CE/IVD) embedded into MylaTM
Step 1Operator
Information
Step 3
Step 2
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V I T EK MS Sim p l i f ie d U s e r G u id e V e r si o n 4 p a g e 7
Material & consumables
For Bacteria & Fungi
Pipette ( 0.1-2.5l) + tips without filter
Loops 1l
VITEK MS-DS Target Slides 48 spots ref 410893
Calibrant : LyfoCults Plus E.coli ATCC 8739 Ref. 301145
Columbia agar + 5 % Sheep blood Ref. 43 041 / 43 049
or Trypcase Soy agar + 5 % Sheep blood Ref. 43 001 / 43 009
Matrix: VITEK MS-CHCA ref 411071
For Molds & Mycoplasma
Please refer to :
For Yeast
VITEK MS-FA ref 411072
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V I T EK MS Sim p l i f ie d U s e r G u id e V e r si o n 4 p a g e 8
ProtocolIdentification VITEK MS
& AST VITEK 2Prep Station
Matrix deposit
1L CHCA *
Calibrant
Deposit
+ Matrix
Step 2
Step 4
A B
Step3
McF
* Prior extraction for yeasts only: 0,5L Formic acid deposit
Step 1
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V I T EK MS Sim p l i f ie d U s e r G u id e V e r si o n 4 p a g e 9
ProtocolIdentification VITEK MS only
Prep Station
Matrix deposit
1L CHCA *
Calibrant
Deposit
+ Matrix
Step 2
Step 3
* Prior extraction for yeasts only: 0,5L Formic acid deposit
O
therisolates
OtherAcquisitiongroups
Step 1
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V I T EK MS Sim p l i f ie d U s e r G u id e V e r si o n 4 p a g e 1 0
ProtocolAST VITEK 2 only
Prep Station
McF
* Prior extraction for yeasts only: 0,5L Formic acid deposit
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V I T EK MS Sim p l i f ie d U s e r G u id e V e r si o n 4 p a g e 1 1
6 .Scan the
VITEK2 Card
barcode andplace the card
on the cassette
1 . Scan the
target slide
2 . Scan the Lab
ID on the plate
3 . Choose
between
Bacteria or Fungi
4 . Deposit the
spots
5 . Prepare thebacterial McF
suspension
7 . F4 Validate
Prep Station
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V I T EK MS Sim p l i f ie d U s e r G u id e V e r si o n 4 p a g e 1 2
Slides Composition
Homepage
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V I T EK MS Sim p l i f ie d U s e r G u id e V e r si o n 4 p a g e 1 3
Acquisition Station
1. Open the door
2. Scan the
target slides
barecodes
3. VITEK MS
retrieves target
slides
information
4. Load the
target slides into
the VITEK MS
5. Start the
Acquisition
6. Acquisition in
progress
7. End of
acquisition
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V I T EK MS Sim p l i f ie d U s e r G u id e V e r si o n 4 p a g e 1 4
Acquisition Station:Spots color code
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V I T EK MS Sim p l i f ie d U s e r G u id e V e r si o n 4 p a g e 1 5
Acquisition Station:Spectra features
Average of all the spectra
collected for the sample
Averaged data afterprocessing
(smoothing, threshold
substraction, peak
detection)
Peak list sent to the MS-ID
server
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V I T EK MS Sim p l i f ie d U s e r G u id e V e r si o n 4 p a g e 1 6
Results to be reviewed
Detail of VITEK MS ID to be
reviewed
Homepage
Technologist Dashboard
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V I T EK MS Sim p l i f ie d U s e r G u id e V e r si o n 4 p a g e 1 7
Refresh the list of results
Review selected identif ication results.
The results are removed from this list
and will be displayed in the Reviewed tab
Review all identif ication results with
a high confidence level.
The results are removed from this list
and will be displayed in the Reviewed tab
Discard selected identification results.
The results are removed from this list
and will be displayed in the Reviewed tab
Enter and assign a comment for theselected result (s) if applicable.
Print a report file of the selected
result(s) to be reviewed
Results to be reviewed
Indicates that an organism is a highly pathogenicorganism or a critical pathogen
This result must be review using the following icon
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V I T EK MS Sim p l i f ie d U s e r G u id e V e r si o n 4 p a g e 1 8
Results to be reviewed
Rules for Consolidation :
-The consolidated identification result can be sent as it is.
- Consolidate the identification by selecting a result
- Result cannot be consolidated, repeat the analysis
(1) Select the
organism name(2) Validate the selected
organism name
Optional: Display a pop-up
window to enter comments
for the selected result(s)
Indicates that an organism has been selected
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V I T EK MS Sim p l i f ie d U s e r G u id e V e r si o n 4 p a g e 1 9
Results to be reviewed
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V I T EK MS Sim p l i f ie d U s e r G u id e V e r si o n 4 p a g e 2 0
Results to be approvedOnly if the 'Review and Approve for result
Validation' setting is enabled in MYLA
Detail of VITEK MS ID to be
approved
Homepage
ManagerDashboard
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V I T EK MS Sim p l i f ie d U s e r G u id e V e r si o n 4 p a g e 2 1
Refresh the list of results
Approve the selected result (s)
The results are removed from this list andwill be displayed in the Approved tab
Approve all ident ification resul ts with
a high confidence level
The results are removed from this list
and will be displayed in the Approved tab
Reject the selected result(s)
The rejected results are removed from this
list and will be displayed in the To be
reviewed tab with the status To rework
Display a pop-up window to enter
comments for the selected result(s)
Print a report file of the selected
result(s) to be approved
Results to be approvedOnly if the 'Review and Approve for result
Validation' setting is enabled in MYLA
When a comment is entered, the button
is displayed on the result line
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V I T EK MS Sim p l i f ie d U s e r G u id e V e r si o n 4 p a g e 2 2
Electronic SignatureOnly if the Electronic Signature
option setting is enabled in MYLA
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When you review, discard, approve & reject result(s), an Electronic Signature
has to be entered to confirm the sending of results.
The 1st time, you have to enter the User name and Password, then only thePassword is required.
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V I T EK MS Sim p l i f ie d U s e r G u id e V e r si o n 4 p a g e 2 3
AST Review
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V I T EK MS Sim p l i f ie d U s e r G u id e V e r si o n 4 p a g e 2 4
VITEKMS Users maintenance
Maintenance Operation Frequency Refer to
Cleaning the VITEK MS
and the Acquisition Station
+ Acquisition Station monitor
To be established according your
internal procedure .
To be written in your SOP
Chapter 5 Maintenance
Cleaning the Adapter To be established according yourinternal procedure .
To be written in your SOP
Changing the desiccant When the desiccant turns to pink
Cleaning of the O-ring seal & thesealing face of the door To be established according your
internal procedure .
To be written in your SOP
Chapter 12