4-Color Multi Laser Engines...4 (other wavelengths such as 375nm, 415nm, 425nm, 473nm, 660nm, 685nm,...
12
4 www.toptica.com iChrome MLE 4-Color Multi Laser Engines Microscopy Flow Cytometry High Throughput Screening Microplate Reader Metrology 405 nm 445 nm 488 nm 640 nm 515 nm 532 nm 561 nm A Passion for Precision.
Transcript of 4-Color Multi Laser Engines...4 (other wavelengths such as 375nm, 415nm, 425nm, 473nm, 660nm, 685nm,...
4 www.toptica.com
iChrome MLE 4-Color Multi Laser Engines
Microscopy
Flow Cytometry
High Throughput Screening
Microplate Reader
Metrology
405 nm
445 nm
488 nm
640 nm
515 nm
532 nm
561 nm
A Passion for Precision.
5 www.toptica.com
© T
ILL
Pho
toni
cs
Contents
Multi Laser Engine Technology ......................................... 3
iChrome MLE-L ........................................................... 4 - 5iChrome MLE-S ........................................................... 6 - 7
Fiber Delivery ..................................................................... 8Control Features ................................................................ 9 COOLAC Technology ......................................................... 10Mechanical Drawings ...................................................... 11
3
Many biophotonics applications require powerful multi-color laser radiation in order to excite various dyes with high spectral selectivity. To this end, “breadboard solutions” are up to now widely employed: several laser sources are combined with dichroic mirrors on an optical table. However, these setups are prone to misalignment and highly sensitive to environmental changes. A much more favorable solution with respect to reliability, compactness and robustness is the iChrome MLE – a true and complete OEM device for demanding multi-color applications. Up to four different wavelengths from a wide range of lasers from UV to IR can be selected according to the desired application. Two versions of the system are available: the iChrome MLE-S, a compact all-diode laser system, comprising up to 4 different diode lasers and the iChrome MLE-L, with up to 3 different diode lasers and one DPSS laser fully integrated in one single box. In both systems, the individual lasers are efficiently combined and delivered through one single mode, polarization maintaining fiber (all-in-one PM/SM fiber output).
Both systems feature TOPTICA’s COOLAC technology. This exceptional technique ensures a Constant Optical Output Level and provides unprecedented fiber coupling efficiency. To compensate for misalignments due to mechanical stress and environmental changes such as temperature or vibrations, the auto-recalibration automatically aligns the system with a single push of a button. Thus, no manual realignment is necessary any longer, offering true drop-shipment capability and ensuring exceptional long term power stability. Furthermore, the iChrome MLE is microprocessor controlled and thus enables flexible OEM integration. High speed analog and digital modulation allow fast switching of laser wavelength and intensity, e.g. for minimal exposure of the sample reducing phototoxicity and bleaching of the fluorescent dye.
With highest output levels, exceptional power stability and fast speed modulation, instruments like confocal microscopes, high content screening systems or flow cytometers will greatly benefit of these compact and reliable multi-color laser engines.
Next Generation Laser Technology - Fully Featured Multi-Color Engines
Contents
Multi Laser Engine Technology ......................................... 3
iChrome MLE-L ........................................................... 4 - 5iChrome MLE-S ........................................................... 6 - 7
Fiber Delivery ..................................................................... 8Control Features ................................................................ 9 COOLAC Technology ......................................................... 10Mechanical Drawings ...................................................... 11
4 www.toptica.com
(other wavelengths such as 375nm, 415nm, 425nm, 473nm, 660nm, 685nm, 785nm, 830nm on request)
Spectral clean-up filter for up to 3 diode lasers (standard) All wavelengths can be triggered simultaneously indepen-dently with asynchronous pulse signals
Excellent power stability under varying ambient temperature
iChrome MLE-LMulti Laser Engine
Key features
• MultiLaserEnginewithupto4laserlines• Single-mode,polarizationmaintainingfiberoutput• Wavelengthsdiodelasers:405nm,445nm,488nm,640nm (375nm, 473nm, 515 nm, 660nm, 785nm and others on request)• WavelengthsDPSSlaser:532nm,561nm (505nm, 515nm, 594nm and others on request)• COOLAC technology for highest coupling efficiency, ultimate stability and drop shipment capability• Directmodulationandfastswitchingbetweenwavelengths• Trueone-boxsolutionwithintegratedelectronics• Uniquefeatures:COOLAC, FINE and SKILL technology• Mostcompactandcosteffectivesolutionformulti-color biophotonic applications
0 3 6 9 12 15 18 21 240
20
40
60
80
100
120
405 nm 488 nm 561 nm 640 nm
Pow
er [%
]
Time [h]
10
15
20
25
30
35
40
45
50
55
60
Am
bien
t Tem
pera
ture
[°C
]
Time
Inte
nsity
405nm
488nm
515nm
640nm
iChrome MLE-L up to 3 diode lasers and 1 DPSS laserStandard Wavelength Sets
LFA 405nm 488nm 561nm 640nm
LFB 445nm 488nm 561nm 640nm
LFC 405nm 488nm 532nm 640mn
LFD 488nm 561nm 640nm
LFE 445nm 515nm 561nm 640nm
5
Key specifications - iChrome MLE-L
Optical specifications Units Diode Lasers DPSS lasers
Available wavelengths* nm 405 nm 445 nm 488 nm 515 nm 640 nm 532 nm 561 nm
Wavelength range nm ± 4 ± 5 + 1 /- 5 ± 5 + 5 /- 3 - -
Fiber coupled output power mW 50 25 40 20 50 30 30
Opticalclean-upfilter:bandwidth/transmission < 10 nm / > 90 % - -
Power stability (drift over 24 h @ +/- 3°C) % ± 2
RMS noise (10 Hz – 10 MHz) % < 0.2
COOLAC calibration time (better 98 %;15 - 40°C) ** s < 2, each wavelength
Fiber output connector FC/AFC (standard), FC/APC compatible, SC (optional), others on request
Fiber cable length (typ.) m 2
Fiber cable type 3 mm Kevlar reinforced PVC
Fiber type single mode, polarization maintaining
Long-term output power average** %/1000 h < 5 < 5 < 2 < 2 < 2 < 2 < 2
M2 < 1.1
Modefielddiameter(typ.) µm 3 3 3.5 3.5 4.2 3.5 3.5
Numerical aperture (5%) (typ.) 0.07 0.06 0.06 0.06 0.05 0.06 0.05
Polarization ratio (typ.) > 100 :1, Linear
Polarization orientation referred to index key ± 3°
Electronic specifications
Digital modulation
Supported digital signal TTL
Maximum digital modulation frequency MHz 20 (asynchronous, each wavelength can be adressed independently)
Rise/Fall time (10% - 90%) (typ.) ns < 5 < 15
Trigger to light (typ.) ns < 50 < 500
Digital modulation extinction ratio ∞, complete on/off > 106
Analog modulation
Maximum analog modulation frequency MHz 1 1
Modulation depth (extinction ratio) % ∞ > 106
General and environmental specifications
Qualified CE
CDRHClassification Class IIIb
RoHS compliancy yes
ESD protection Level 4
DC input requirements 12 V DC, 6 A
Power consumption W < 50
Digital communication interface RS 232 (≤ 115.200 baud), Ethernet
Operating temperature range °C 15 .. 40
Storage temperature range °C -10 .. 60
Operating relative humidity (non-condensing) % < 90
Dimensions (L x W x H) mm3 295 x 248 x 110
Dimensionslaserheadwithfiberminimumbendradius (L x W x H)
mm3 295 x 320 x 110
Weight kg 7
* other wavelengths such as 375nm, 395nm, 420nm, 473nm, 594nm, 660nm, 685nm, 785nm, 830nm on request** with COOLAC Technology
6 www.toptica.com
Key specifications - iChrome MLE-S
Optical specifications Units Diode Lasers
Available wavelengths* nm 405 nm 445 nm 488 nm 515 nm 640 nm
Wavelength range nm ± 4 ± 5 + 1 /- 5 ± 5 + 5 /- 3
Fiber coupled output power mW 50 25 40 20 50
Opticalclean-upfilter:bandwidth/transmission < 10 nm / > 90 %
Power stability (drift over 24 h @ +/- 3°C) % ± 2
RMS noise (10 Hz – 10 MHz) % < 0.2
COOLAC calibration time (better 98 %;15 - 40°C) ** s < 2, each wavelength
Fiber output connector FC/AFC (standard), FC/APC compatible, SC (optional), others on request
Fiber cable length (typ.) m 2
Fiber cable type 3 mm Kevlar reinforced PVC
Fiber type single mode, polarization maintaining
Long-term output power average** %/1000 h < 5 < 5 < 2 < 2 < 2
M2 < 1.1
Modefielddiameter(typ.) µm 3 3 3.5 3.5 4.2
Numerical aperture (5%) (typ.) 0.07 0.06 0.06 0.06 0.05
Polarization ratio (typ.) > 100 :1; Linear
Polarization orientation referred to index key ± 3°
Electronic specifications
Digital modulation
Supported digital signal TTL
Maximum digital modulation frequency MHz 20 (asynchronous, each wavelength can be adressed independently)
Rise/Fall time (10% - 90%) (typ.) ns < 5
Trigger to light (typ.) ns < 50
Digital modulation extinction ratio ∞, complete on/off
Analog modulation
Maximum analog modulation frequency MHz 1
Modulation depth (extinction ratio) % ∞
General and environmental specifications
Qualified CE
CDRHClassification Class IIIb
RoHS compliancy yes
ESD protection Level 4
DC input requirements 12 V DC, 6 A
Power consumption W < 50
Digital communication interface RS 232 (≤ 115.200 baud), Ethernet
Operating temperature range °C 15 .. 40
Storage temperature range °C -10 .. 60
Operating relative humidity (non-condensing) % < 90
Dimensions (L x W x H) mm3 201 x 248 x 110
Dimensionslaserheadwithfiberminimumbendradius (L x W x H)
mm3 201 x 320 x 110
Weight kg 7
* other wavelengths such as 375nm, 395nm, 420nm, 473nm, 594nm, 660nm, 685nm, 785nm, 830nm on request** with COOLAC Technology
7
(other wavelengths such as 375nm, 415nm, 425nm,
473nm, 660nm, 685nm, 785nm, 830nm on request)
All lasers can be triggered simultaneously independently with asynchronous pulse signals
Spectral clean-up filter for up to 4 diode lasers (standard) Trigger to light < 50 ns
iChrome MLE-SAll-Diode Multi Laser Engine
Key features
• MultiLaserEnginewithupto4diodelaserlines• Availablewavelengths: 405nm, 445nm, 488nm, 515nm, 640nm (375nm, 473nm, 660nm, 785nm and others on request)• Single-mode,polarizationmaintainingfiberoutput• Highfibercoupledoutputpowerlevels• COOLACtechnologyforhighestcouplingefficiency,ultimate stability and drop shipment capability• Directmodulationandfastswitchingbetweenwavelengths• Trueone-boxsolutionwithintegratedelectronics• Uniquefeatures:COOLAC, FINE and SKILL technology• Mostcompactandcosteffectivesolutionformulti-color biophotonic applications
Time
Inte
nsity
405nm
488nm
515nm
640nm
Trigger signal
Laser light
(other wavelengths such as 375nm, 415nm, 425nm,
473nm, 660nm, 685nm, 785nm, 830nm on request)
iChrome MLE-S up to 4 diode lasers
Standard Wavelength Sets
SFA 405nm 445 nm 488nm 640nm
SFB 405nm 488nm 640nm
SFC 405nm 445 nm 488nm
SFD 405nm 445 nm 515nm 640nm
SFE 445 nm 515nm 640nm
SFF 405nm 445 nm 488nm 515nm
8 www.toptica.com
Fiber Delivery
After efficiently combining the individual laser lines, the iChrome MLE delivers the laser light via one single mode, polarization maintaining fiber. In order to avoid degradation by irradiation in the UV regime, special long-life fibers are employed.
By default the system is equipped with an FC/AFC (8° angled) connector. For convenient OEM integration other output options are available on request.
Possible Fiber outputs are: - FC/AFC connector (standard) - (e.g. FC/APC, FC/PC, SC,...) on request
(FC/AFC)
Fiber length: 2 meter
connector index key
8°
± 2°
fast axis
slow axis aligned to index key
± 2°
(FC/AFC)
Fiber length: 2 meter
connector index key
8°
± 2°
fast axis
slow axis aligned to index key
± 2°
(FC/AFC)
Fiber length: 2 meter
connector index key
8°
± 2°
fast axis
slow axis aligned to index key
± 2°
9
Control Features
1
92 34
6710
8
5
11
12
The iChrome MLE requires only one control interface in order to operate all laser lines simultaneously. For this purpose, a graphical user interface is included by default, which gives full control for the individual laser lines and allows operating the iChrome MLE as stand-alone system.
For easiest and flexible OEM integration, the system is equipped with all established communication interfaces. Communication can take place e.g. via RS 232, TCP/IP or via programmable in- and outputs of a DB-15 connector.
CONNECTORS
1 Power input DIN-5 561 nm
2 Aux input SMB-connectorr Analog, 0..+5V
3 Ethernet RJ45 – 8P8C 640 nm
4 Digital input (laser 1 - 4) SMB-connector TTL
5 Analog input (laser 1 - 4) SMB-connector Analog, 0..+5V
6 I/O-A DB-9 female RS 232 input
7 I/O-B DB-9 male RS 232 output
8 I/O-Aux DB-15 femaleInterlock,
programmable in-/ and outputs
INDICATORS
9 LEDs for general system status (laser, ready, error, power)
10 Display for laser specific status
GENERAL
11 Key switch
12 Fiber out
10 www.toptica.com
COOLAC TechnologyUnique Solution for High Power Hands-Off Fiber Coupled Lasers
Aligning and focusing a laser beam into ultra tiny single mode fibers (down to core diameters of 2.5 µm) with highest efficiency is utmost challenging: Laser pointing fluctuations, deviations in the beam path and smallest thermal or mechanical drifts in the fiber mounting can contribute to a significant loss of fiber coupling efficiency and thus in decreased output power. Up to now manual realignment by a trained technician was required to recalibrate the laser output power from time to time.
TOPTICA‘s unique solution for high power single-mode permanently fiber coupled lasers is the COOL technology (Constant Optical Output Level). This patent pending
fiber coupling concept is novel to the industry, and does not use any mechanical micro adjustable parts, which normally are the first to shift due to varying ambient conditions (e.g. temperature changes or vibrations). All major optical components are solidly mounted and the optical micro alignment is unsusceptible to mechanical and thermal distortions or translations.
The auto-calibration feature automatically realigns laser lines of the system with a single push of a button. This ensures full drop shipment capability, push-button installation and exceptional long term operational stability. The result is a multi-color system featuring a high and constant
power level after fiber delivery which is essential for quantitative and reliable data.
COOLAC benefits:•Auto Calibration feature •Combinedfibercouplingvaluesin excess of 50% can be restored by a single push-button command •Dropshipmentwarrantyandpush- button installation: Laser in spec after push-button command •Unsusceptibletothermalfluctuations between 15 and 40 °C •Unsusceptibletoextensivemechanical disturbances •Highestoperationalstability
Performance
COOLAC Technology
0 1 2 3 4 5 6 70
10
20
30
40
50
60
70
80
90
100
110
Powe
r [%
]
Time [h]
10
15
20
25
30
35
40
45
50
55
60
Am
bien
t Tem
pera
ture
[°C]
COOLAC
0
10
20
30
40
50
405 488
60
445Wavelength [nm]
Pow
er [m
W]
515 532 561 640
iChrome MLE standard wavelengths with
highest optical power
Random noise < 0.2 %, all lasers running
10 100 1k 10k 100k 1M 10M-160
-150
-140
-130
-120
-110
-100
-90
-80
-70
-60
RIN
[dB/
Hz]
Frequency [Hz]
iChrome MLE-LFA - Random Intensity Noise
All 4 lasers running: RMS noise 0.13 %
All dimensions are given in mm
11
Mechanical Drawings
All dimensions are given in mm
iChrome MLE-S
iChrome MLE-L
12 www.toptica.com
Distributors
BR
-044
A 2
011-
01
TOPTICA Photonics AGLochhamer Schlag 19D-82166 Graefelfi ng/MunichGermanyPhone: +49 89 85837-0Fax: +49 89 [email protected]
Australia & New ZealandLastek Pty. Ltd.Mr. Alex StancoThebarton CampusUniversity of Adelaide10 Reid Street5031 Thebarton, SAAustralia Phone: +61 8 8443 8668Fax: +61 8 8443 [email protected]
ChinaUniversal (Hong Kong)Technology Co. Ltd.Mr. Alex Cai Room 615-619Capital Group PlazaNo. 6 Chaoyangmen BeidajieBeijing 100027, P.R. ChinaPhone: +86 10 8528 3377Fax: +86 10 8528 [email protected]
FranceOpton Laser InternationalDr. Costel SubranParc Club d‘Orsay Université29, rue Jean RostandF-91893 Orsay Cedex, FrancePhone: +33 1 6941 0405Fax: +33 1 6941 [email protected]
IndiaSimco Global Technology & Systems Ltd.Dr. R. S. Daryan Simco House (Head Offi ce)14 Bhawani KunjBehind Sector D-II Vasant Kunj110017 New Delhi, IndiaPhone: +91 11 2689 9867Fax: +91 11 2612 [email protected]
IsraelLahat Technologies Ltd. Mr. Kfi r Ben-YehudaTeradion Industrial ZoneM.P. Misgav, 20179, Israel Phone: +972 4 999 0151Fax: +972 4 999 [email protected]
JapanINDECO, INC.Mr. Kazunobu Takahashi1-11-14, Kasuga, Bunkyo-kuTokyo 112-0003, Japan Phone: +81 3 3818 4011 Fax: +81 3 3818 4015
Sonezaki Sawada Bld. 6F2-1-13, Sonezakishinchi,Kita-ku,Osaka 530-0002, JapanPhone: +81 6 6341 5799 Fax: +81 6 6341 5798
KoreaJINSUNG LASERMr. Ha-Won Lee#535-5, Bongmyung-DongYusung-GuHanjin Offi cetel Rm# 1016Daejeon, 305-301, South KoreaPhone: +82 42 823 5300Fax: +82 42 823 [email protected]
Singapore / MalaysiaPrecision Technologies Pte LtdMr. Vincent Chan211 Henderson Road # 13-02Henderson Industrial ParkSingapore 159552Phone: +65 6273 4573Fax: +65 6273 [email protected]
TaiwanSLEO Photonics Co. Ltd. Mr. Jimmy Chao6F, No. 2, Lane 74An-der StreettHsin Tien City, Taipei CountyTaiwan 231, R.O.C.Phone: +886 2 2211 5408Fax: +886 2 2211 [email protected]
TurkeyCS Analytical LaboratoryEquipments LimitedMr. Ercan AcikalinErgin Sokak No: 27/506580 Mebusevier - Tandogan Ankara, TurkeyPhone: +90 312 223 0302Fax: +90 312 223 [email protected]
United Kingdom & IrelandMr. Howard Potter Unit 4H Lansbury Estate Woking, Surrey, UK, GU21 2EP Great BritainPhone: +44 1483 799 030 Fax: +44 1483 799 [email protected] www.toptica.com
USA & CanadaTOPTICA Photonics, Inc.1286 Blossom DriveVictor / Rochester, NY 14564U.S.A.Phone: +1 585 657 6663Fax: +1 877 277 [email protected]
Every other country not listed:
TOPTICA Photonics AGLochhamer Schlag 19D-82166 Graefelfi ng / MunichGermanyPhone: +49 89 85837-0Fax: +49 89 [email protected]
TOPTICA Photonics, Inc.1286 Blossom DriveVictor / Rochester, NY 14564U.S.A.Phone: +1 585 657 6663Fax: +1 877 277 [email protected]
