3C pro wt CM IEALFQ↓GPPKFR 3C pro mut CM IE K LFQ P PPKFR
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41 3C pro wt CM IEALFQ↓GPPKFR 3C pro mut CM IEK LFQ P PPKF R GFP 2 DsRed 2 Figure S1. Diagram depicting the FRET probe for detecting the 3 C pro activity in vivo. Composition and linear arrangement of the G FP 2 and DsRed2 reporters are illustrated. The 3C pro cleavage moti f (3C pro wt CM) and the corresponding mutant motif (3C pro mut CM) in the linker are shown, with the arrow indicating the scissile bond of 3C pro and the underlined residues representing the mutat ions in the P4 and P1’ positions.
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DsRed2. GFP 2. 3C pro wt CM IEALFQ↓GPPKFR 3C pro mut CM IE K LFQ P PPKFR. - PowerPoint PPT Presentation
Transcript of 3C pro wt CM IEALFQ↓GPPKFR 3C pro mut CM IE K LFQ P PPKFR
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3Cpro wt CM IEALFQ↓GPPKFR
3Cpro mut CM IEKLFQ PPPKFR
GFP2 DsRed2
Figure S1. Diagram depicting the FRET probe for detecting the 3Cpro activity in vivo. Composition and linear arrangement of the GFP2 and DsRed2 reporters are illustrated. The 3Cpro cleavage motif (3Cpro wt CM) and the corresponding mutant motif (3Cpro mut CM) in the linker are shown, with the arrow indicating the scissile bond of 3Cpro and the underlined residues representing the mutations in the P4 and P1’ positions.