3 Ways our New LC-MS Triple Quads Improve

Residue Analysis

Complex Matrices

Large Sample Numbers

Low Concentration

Today’schallenges in quantitative mass spectrometry

demanded that we elevate

triple quad capabilities

Small Sample Amounts

Extreme quantitative value with best-in-class sensitivity

Thermo Scientific™TSQ Endura MS

Thermo Scientific™TSQ Quantiva MS

Extreme quantitative performance with unrivaled attogram level sensitivity

New GenerationTriple Quadrupole LC-MS systems

How do our new Triple Quadrupole systems improve residue analysis?

SPEED

ChallengeHigh volume multi-residue analyses in short run times

Solution500 SRMs per second

9.9 SRM/sec 47 SRM/sec 166 SRM/sec 333 SRMs/sec 500 SRMS/Sec0

2000000

4000000

6000000

8000000

10000000

12000000

14000000

Pea

k A

rea

Traditional Triple Quads decline in sensitivity, even at lower SRMs per second

New Thermo Scientific™ Triple Quadrupole systems maintain sensitivity up to 500 SRMs per second

High sensitivity at every speed

More points across the peak means

more reproducible results and allows

for faster run times

1.75 1.8 1.85 1.9 1.95 2 2.05 2.10

1000000

2000000

3000000

4000000

5000000

6000000

250 Transitions Monitored Simultaneously

167 SRMS/second 500 SRMS/second

Time (minutes)

Sig

nal

3 points across the peak

10 points across the peak

Analysis of 250 pesticides> 500 SRMs 5 ppb in onion matrix  

Scans Across Peaks 500 SRMs simultaneously (not scheduled/timed)

Sufficient number of data points for reproducible assays

Comparison of Area Response vs. Scan Speeds

0.8 1 2 5 10 20 500.00E+00

1.00E+07

2.00E+07

3.00E+07

4.00E+07

5.00E+07

6.00E+07

7.00E+07

AtrazineAzoxystrobinCarbendazimSimazine

Dwell time (ms)

Ave

. Are

a

10 ppb in Onion Matrix

SENSITIVITY

ChallengeDemanding assays requiringlowest limits of detection

Unrivaled triple quad sensitivity

Solution

Unrivaled quantitation on TSQ Quantiva MS

Pesticides in extracted spinach matrix

Benfuracarb (10 ppq LOD and 4.5 orders dynamic range) Chromatograms - CV%:11.8%

LOQ (ppt) – Veterinary Drug Residues in Milk (n=7) on TSQ Quantiva MS

Compound LOD (ppt) % RSD LOQ (ppt) % RSD

Albendazole 2.47 12.64 12.34 4.11

Oxfendazole 2.44 10.86 12.20 6.89

Sulfadiazine 23.81 17.5 119.05 9.05

Sulfamethazine 12.62 10.62 25.24 10.21

Sulfaquinoxaline 5.98 7.29 11.97 2.47

Thiabendazole 24.19 3.48 48.37 1.65

Tilmicosin 121.79 1.05 243.57 2.16

Trimethoprim 11.91 20.91 23.81 10.12

Tylosin 11.87 12.79 23.74 10.75

Exceeds EU Regulation No 37/2010100–1000x

Low and Sub-ppt Levels

• Experiment: Analysis of reclaimed waste water for emerging contaminants (PPCPs)*

• TIC of 1.75 pg/mL injected standard 1 mL injection with Thermo Scientific™ EQuan MAX Plus™ online sample prep LC-MS with TSQ Quantiva MS

• Calibration range:0.06 pg/mL – 1000 pg/mL with a 1mL injection, that’s 60 fg – 1000 pg on column.

0

10

20

30

40

50

60

70

80

90

100

Re

lati

ve A

bu

nd

ua

nce

9.68

9.79

10.266.87

10.37

11.269.46

8.177.856.63

11.516.01 12.425.844.34

4 5 6 7 8 9 10 11 12 13

Time (min)

*Lee Ferguson and Gordon Getzinger, Environmental Chemistry, Duke University, USA

Calibration range, 0.06 – 1000 pg/mL - Flutolanil

60fg On Column

4.5 orders of magnitude

Ion Ratio overlay

Metoprolol and Oxadiazon

LOD 100 ppq Metoprolol

Tilt LOD 100 ppq

LOD 50 ppq Atraton

Calibration range: 0.1-100 ppt

Calibration range: 0.05-100 ppt

Calibration range: 0.01-100 ppt

Quantitation results using Equan MAX Plus System with TSQ Quantiva MS

Compound LOD (pg/mL) Compound LOD (pg/mL)Acephate 0.24 Fluridone 0.12Allethrin 7.8 Flutolanil 0.06Ametryn 0.12 Formasulfron 0.12

Atraton 0.12 Halosulfron-methyl 0.12Atrazine 0.12 Imidacloprid 0.06

Atrazine-desethyl 0.12 Iprodione_a 15.63Atrazine-desisopropyl 0.24 Iprodione_b 31.25

Azoxystrobin 0.12 Metalaxyl 0.06Benzotriazole 7.8 Metoprolol 0.24Bioresmethrin 62.5 Oxadiazon 3.9

Bloc (Fenarimol) 0.24 Pramoxine 0.12Carbaryl 0.12 Prometron 0.12

Carbendazim 0.12 Propanmide 0.12DEET 0.98 Quinclorac 7.8

Etofenprox 3.9 Thiencarbazone-methyl 3.9

Fenamiphos 0.12 Thiophanate-methyl 0.24Fluoxastrobin 0.5 Tramadol 0.06

ROBUSTNESS

ChallengeRobust performance for ongoing reliability

SolutionBuildup prevention by

elimination of neutral clusters

Are

a

Injections

Robustness across >350 injections with pesticide spiked sample on TSQ Endura MS

Initial calibration curve overlaid with calibration

curve acquired after 300 injections of pesticide spiked

sample on TSQ Endura MS (Imidacloprid and

2,4-D)

Robustness across 1600 injections

Ave. %RSD 3.5

• Tuesday: 10 ppb standards in solvent

• Wednesday and Friday: 10 ppb standards in jalapeno matrix

• LC Run time: 8 minutes

Ave. %RSD 3.3

0.00E+00

5.00E+06

1.00E+07

1.50E+07

2.00E+07

2.50E+07

3.00E+07

0 200 400 600 800 1000 1200 1400 1600 1800

tues

weds

fri

0.00E+00

5.00E+06

1.00E+07

1.50E+07

2.00E+07

2.50E+07

3.00E+07

3.50E+07

4.00E+07

4.50E+07

5.00E+07

0 200 400 600 800 1000 1200 1400 1600 1800

tues

weds

fri

Are

aA

rea

Injection number

Injection number

How are our systems delivering these

capabilities?

Active Ion Management

Precision design of all electric fields, optimized in concert, to produce maximum signal and to prevent contamination.

AIM TECHNOLOGY

The design behind the capabilities

Novel Neutral Blocker

Captures contaminating species outside of the ion path, keeping ion optics cleaner

Tool-less Removal

No tools needed to remove ion optics, facilitating quicker cleaning

Re-designed TSQ Triple Quadrupole Platform

Extreme quantitative value with best-in-class sensitivity

TSQ Endura MS

TSQ Quantiva MSExtreme quantitative performance with unrivaled attogram level sensitivity

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