3 Ready to use Media - Tulip Group Ready to use Media.pdf · 2011-02-23 · Microxpress Dehydrated...
Transcript of 3 Ready to use Media - Tulip Group Ready to use Media.pdf · 2011-02-23 · Microxpress Dehydrated...
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There is generally recognized need for methods that permit rapid estimation of the
bacteriological quality of water. Applications of rapid method may range from
analysis of waste water to potable water quality assessment. Ideally, rapid
procedures are reliable and have sensitivity levels equal to those of the standard
tests routinely used. However, sensitivity of a rapid test may be compromised
because the bacterial limit sought may be below the minimum bacterial
concentration essential to rapid detection. Tests for detection of indicator
organisms, rather than pathogens are used generally in water testing. The
coliform group of bacteria is the principle indicator of suitability of water for
domestic, industrial or other uses.
Our Water Testing kits are designed to provide rapid, accurate, easy to use and
cost effective solutions to a wide variety of applications for water testing. Though
simple to use, they provide the latest in bacterial diagnosis technology to provide
consistent reliable results.
Water Testing SolutionsColiforms, E.coli and Enterococci are important indicators of faecal pollution in
water analysis. Some of the tests being dependent on enzyme substrate reactions,
these tests utilize hydrolysable substrates for detection of the above organisms.
When the enzyme technique is used, the total coliform group is defined as all
bacteria possessing the enzyme beta-D-galactosidase, which cleaves the
chromogenic substrate, resulting in release of the chromogen. E.coli are defined
as bacteria giving a positive total coliform response and possesseing the enzyme
beta-D-glucuronidase, which cleaves a fluorogenic substrate, resulting in the
release of the fluorogen. Enterococci possess the enzyme beta-D-glucosidase,
which cleaves the chromogenic substrate, resulting in release of the chromogen.
For procedures, storage, references, results and other information, refer
to the package insert of individual product.
Summary and Principle
The test includes detection of E.coli and coliforms based on enzyme substrate
reaction.
The coliform group of bacteria is the principle indicator of suitability of water for
domestic, industrial or other uses.
E.coli can be presumptively identified by the production of beta-
glucuronidase(GUD) which has been proposed as an alternative for IMViC tests.
The medium contains all nutrients required for the growth of coliforms and E. coli
Rapid Coliform Test Kitalong with the substrate. The non-fluorescent substrate is cleaved by the enzyme
GUD produced by E.coli to release the fluorogen (4-methylumbelliferone), which
exhibits a bluish fluorescence when exposed to long wave (366nm) ultraviolet
light. Coliforms, on the other hand, will not produce fluorescence.
Cultural Response
Organism (ATCC) Colour Fluorescence
E.aerogenes (13048) Blue green -
E.coli (25922) Blue green +
Summary and Principle
This kit is used for detection of coliforms in water from treatment plants or
distribution systems, the test being only presumptive.
The presence absence test for the coliform group is a simple modification of the
multiple tube procedure. Simplification, by use of one large test portion (100 ml)
in a single culture bottle to obtain qualitative information on the presence or
absence of coliforms, is justified on the theory that no coliforms should be present
in 100 ml of a drinking water sample
The medium contains nutrients required for the growth of coliforms. Lactose is the
PA Coliform Test Kitfermentable carbohydrate. Phosphates provide buffering action while sodium
lauryl sulphate inhibits organisms other than coliforms. Bromocresol purple is the
pH indicator which turns yellow at acidic pH.
Cultural Response
Organism (ATCC) Growth Colour
E.aerogenes (13048) Luxuriant Light yellow
E.coli (25922) Luxuriant Yellow
E.faecalis (29212) Inhibited -
K.pneumoniae (13883) Luxuriant Yellow
S.typhi (6539) Luxuriant No change (purple)
Summary and Principle
The test includes identification and differentiation of Enterococci from water
samples based on enzyme substrate reaction.
Rapid Enterococci Test KitThe presence of Enterococcus group, which is a subgroup of faecal streptococci,
serves as a valuable bacterial indicator for determining the extent of faecal
contamination and it is more specific than the detection of coliforms which may
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Summary and Principle
This kit is used for simultaneous detection of Salmonella, Vibrio species and E.coli
based on H S production.2
Hydrogen sulphide can be produced at least in small amounts from sulphur
containing amino acids. Some bacteria are capable of breaking down sulphur
containing amino acids (cystine, methionine) or reducing inorganic sulphur
containing compounds (sulfate, thiosulphate) to produce H S. This reduced 2
sulphur may then be incorporated into other cellular amino acids, or perhaps into
coenzymes. The medium incorporates sulphur containing compounds and iron
salts. The organisms reduce this sulphur containing compounds to sulphite and
H S gas by virtue of a reductive enzyme. Production of H S gas is detected as an 2 2
Rapid H S Kit2
insoluble black precipitate of ferrous sulphide formed upon reaction of H S with 2
iron salts. Formation of H S indicates presence of unwanted microorganisms 2
making the water unfit for drinking.
Cultural Response
Organism (ATCC) Growth H S production2
C.freundii (8090) + +
E.coli (25922) + -
P.mirabilis (25933) + +
P.vulgaris (13315) + +
S.typhi (6539) + +
V.cholerae (15748) + +
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Microbiological testing is both time-and labor intensive, which is why there was a
need to develop easier, faster methods for detecting microorganisms.
Our rapid methods for detection of microorganisms offer savings in both time and
labour. These are miniatured iidentification kits for detection of microorganisms
One Step Presumptive Identification Testsbased on various principles like enzyme production, ability to utilize a particular
substrate, detection of metabolic end products, etc.
For procedures, storage, references, results and other information, refer
to the package insert of individual product.
Summary and Principle
The identification is based on fluorescence detection of E. coli using enzyme
substrate reaction.
E.coli are important indicators of faecal pollution. 92- 95% of E.coli possess b-
glucoronidase, which will hydrolyze MUG (4-methylumbelliferyl-b-D-
glucuronide) to yield 4-methylumbelliferone. This compound produces a visible
Rapid Presumptive Identification Kit For E.Coliblue green fluorescence when excited with long wave UV light(366nm).
Cultural Response
Organism (ATCC) Fluorescence
E.aerogenes (13048) -
E.coli (25922) +
originate from non-faecal sources. The medium contains all the essential
nutrients required for the growth of enterococci, and a suitable inhibitor to inhibit
accompanying bacterial flora, specially gram negative organisms. The enzyme
beta-D-glucosidase produced by enterococci cleaves the chromogenic substrate,
resulting in an intensive colour change in the broth from light yellow to blue green.
Cultural Response
Organism (ATCC) Growth Colour
E.coli (25922) Poor Yellow
E.faecalis (29212) Luxuriant Blue
green
Summary and Principle
The identification is based on PYR test. PYR is a rapid test to determine the ability
of the organism to produce the enzyme L-pyrrolidonyl peptidase (PYR). The test
can be used in differentiating streptococcus species. Following incubation with
PYR (L-pyrrolidonyl-b-naphthylamide) substrate, PYR positive streptococci will
Rapid PYR Test Kithydrolyze it to release L-pyrrolidone and b-naphthylamide. On addition of the
PYR Reagent, development of a cherry red colour indicates a positive test.
Cultural Response
Organism (ATCC) Result
S.agalactiae (27956) - (no change)
S.pyogenes (19615) + (red/pink)
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Summary and Principle
The test includes presumptive identification of E.coli on the basis of enzyme
substrate reaction and Indole Test. Indole test alone, is not an adequate screen for
E.coli, since there are at least 52 species of gram negative bacilli that grow on
MacConkey media, that are indole positive and ferment lactose. These include
K.oxytoca, Citrobacter freundii, Serratia odorifera, and Aeromonas hydrophila.
The enzyme-substrate test therefore, in conjunction with Indole test can be used
as a rapid screening test to assist in the identification of E.coli.
Lactose fermentation is a classical identification test. It is normally demonstrated
by acid production after the dissacharide has been cleaved into galactose and
glucose by the enzyme beta-galactosidase.
The medium contains all essential nutrients required for growth of E.coli along
with a synthetic chromogenic substrate ortho-nitrophenyl-beta-D-
Rapid Test Kit For E.Coligalactopyranoside (ONPG), which is a substitute for lactose. ONPG is hydrolysed
in the same way as lactose, but being chromogenic produces a yellow colour.
Using this test system b-galactosidase positive , indole positive, gram-negative
organisms can be presumptively identified as E.coli. Hydrolysis of additive o-
nitrophenyl-b-D-galactopyranoside (ONPG) denotes presence of b-
galactosidase enzyme and is detected by development of yellow color in the
medium. Indole production is detected by addition of Kovac's Reagent.
Development of pink colour is a positive Indole test.
Cultural Response
Organism (ATCC) Result
ONPG Indole
E.aerogenes (13048) + (yellow) -(no change)
E.coli (25922) + (yellow) +(pink)
Summary and Principle
The test is based on detection of esculin hydrolysis and is used for the
identification of group D streptococci. Group D streptococci and enterococci are
able to split esculin.
The medium contains esculin, ferric citrate to provide ferric ions, and 1% oxbile to
inhibit most other strains of non-group D streptococci. Sodium azide is
incorporated into the medium to inhibit gram-negative bacteria. Esculin is
hydrolyzed by group D streptococci to form dextrose and esculetin. Esculetin reacts
with the ferric ions contained within the medium, turning the medium dark brown
Rapid Test Kit For Esculin Hydrolysisto black. Thus, the tolerance to the presence of bile and the hydrolysis of esculin
provide the means to presumptively identify group D streptococci. This test can
also be used for identification of Listeria, since all Listeria species hydrolyse
esculin.Cultural Response
Organism (ATCC) Result (esculin hydrolysis)
L.monocytogenes (19112) +(blackening of medium)
S.bovis (27960) +(blackening of medium)
S.pyogenes (19615) -
Rapid Test Kit For MR-VPSummary and Principle
The test includes detection of acid and acetoin production. Methyl red test detects
the production of sufficient acid during the fermentation of glucose and the
maintenance of conditions such that the pH of an old culture is sustained below
4.4 as shown by a change in colour of the Methyl Red Indicator. Methyl red
positive organisms produce high levels of acid during fermentation of dextrose,
overcome the phosphate buffer system and produce a red color upon addition of
Methyl Red Indicator.
Voges Proskauer test detects the production of acetylmethylcarbinol (acetoin), a
natural product formed from pyruvic acid in the course of glucose fermentation.
The red color produced by the addition of Barritt Reagent A and Barritt Reagent B
tot he cultures is due to the ability of organism to produce a neutral product
acetoin. The acetoin is oxidized in the presence of oxygen and alkali to produce
diacetyl, which reacts in the presence of creatine to produce a red color.
Cultural Response
Organisms (ATCC) Result
MR VP
E.aerogenes (13048) -(yellow) +(red)
E.coli (25922) +red) -(no change)
K.pneumoneae (23357) -(yellow) +(red)
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Summary and Principle
The test is based on the visualization of phenylalanine deamination reaction and
is used to differentiate Proteus and Providencia from enteric bacilli
Members in the genera Proteeae of Enterobacteriaceae have the ability to
deaminate phenylalanine to phenylpyruvic acid by enzymatic activity.
Phenylalanine serves as a substrate for the enzyme, which is able to deaminate it
to form phenylpyruvic acid. The addition of TDA Reagent following incubation
Rapid Test Kit For Phenylalanine Deaminationresults in appearance of deep green color in case of positive reaction or no color
change in case of negative reaction.Cultural Response
Organism (ATCC) Result/Colour
E.aerogenes (13048) -
P.alcalifaciens (9886) +(green)
P.vulgaris (13315) +(green)
Ready Prepared Media
Ready prepared media are both time and labor saving. We offer a variety of ready
media (100 ml) in glass bottles intended for use in food and dairy industry, water
and waste water treatment plants, cosmetics, pharmaceuticals, medicine, etc.
For relevant information like summary, medium formulation, quality
control and results, references, refer to the individual product in
dehydrated media section. For directions of use and storage conditions
refer to the bottle label of individual product.
Alkaline Peptone water is used as an enrichment medium for the cultivation of
vibrio species from clinical and nonclinical specimen. It is also useful for
preliminarily enrichment of vibrios from faeces or other contaminated materials.
Direction
1. Use as per requirements
A highly nutritious general purpose liquid medium for cultivation of variety of
fastidious and non-fastidious microorganisms, including aerobic and anaerobic
bacteria.
Direction
1. Use as per requirements.
Blood Agar Base is a non-selective general-purpose medium to which blood may
be added for use in isolation and cultivation of streptococci, and other fastidious
pathogenic organisms like Neisseria, etc. It is also used for detection of
haemolytic activity.
Directions
1. Loosen the cap.02. Melt the medium in water bath at 100 C.
03. For preparation of blood agar, cool the base to 45-50 C and aseptically add
5% v/v sterile, defibrinated blood.
ALKALINE PEPTONE WATER
BHI BROTH
BLOOD AGAR BASE
4. Mix well and pour plates.
Brain Heart Infusion Agar is a general-purpose medium used for the cultivation of
a wide variety of organisms including bacteria, yeasts and moulds.
Directions
1. Loosen the cap.02. Melt the medium in water bath at 100 C.
3. Mix well to distribute the precipitate uniformly and pour plates.
C.L.E.D. Agar with Andrade indicator is used for the isolation, enumeration and
presumptive identification of microorganisms from urine, giving good colonial
differentiation.
Directions
1. Loosen the cap.02. Melt the medium in water bath at 100 C.
3. Mix well and pour plates.
A basal medium for preparation of blood and chocolate agar and for various
selective and identification media in isolating and cultivating fastidious
microorganisms.
Direction
1. Loosen the cap.02. Melt the medium in water bath at 100 C. Avoid overheating.
03. Cool to 45 C and pour plates.
EMB Agar, Levine is a slightly selective and differential medium used for the
isolation, enumeration and differentiation of members of Enterobacteriaceae.
BRAIN HEART INFUSION AGAR
CLED AGAR WITH ANDRADE INDICATOR
COLUMBIA BLOOD AGAR BASE
EMB AGAR, LEVINE
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Directions
1. Loosen the cap.02. Melt the medium in water bath at 100 C. Avoid overheating.
03. Cool to 45 C and shake the medium in order to restore the blue colour
(oxidize the methylene blue) and to suspend the precipitate which is an
essential part of the medium
4. Pour plates.
Glucose Broth used for the study of cultivation and fermentation studies of
microorganisms and for testing the sensitivity of microorganisms to antibiotics by
the tube dilution method.
Direction
1. Use as per requirements.
Glucose Phosphate Broth is used for differentiation of coli-aerogene group by
means of Methyl Red and Voges Proskauer reactions.
Direction
1. Use as per requirements.
A medium for cultivation of wide variety of bacteria from blood specially
fastidious streptococci and Corynebacterium diptheriae.
Direction
1. Use as per requirements.
MacConkey Agar with Crystal Violet, NaCl and 0.15% Bile Salts is a slightly
selective and differential medium for the detection of coliforms and enteric
pathogens.
Directions
1. Loosen the cap.02. Melt the medium in water bath at 100 C. Avoid overheating.
03. Cool to 45 C and pour plates.
MacConkey Agar without Crystal Violet, NaCl & with Sodium Tauracholate is used
for the cultivation and differentiation of enteric bacteria and potentially
pathogenic gram-positive organisms, while restricting swarming of Proteus
species.
Directions
1. Loosen the cap.02. Melt the medium in water bath at 100 C. Avoid overheating.
03. Cool to 45 C and pour plates.
GLUCOSE BROTH
GLUCOSE PHOSPHATE BROTH
HARTLEY BROTH
MAcCONKEY AGAR WITH CV, NaCl and 0.15% BILE SALTS
MAcCONKEY AGAR W/O CV, NaCl and W/ SODIUM TAUROCHOLATE
MUELLER HINTON AGAR
NUTRIENT AGAR
NUTRIENT BROTH
PEPTONE WATER
PLATE COUNT AGAR
PHOSPHATE BUFFER USP
Mueller Hinton Agar is used for antimicrobial disc diffusion susceptibility testing
of common, rapidly growing bacteria by the Bauer-Kirby method.
Directions
1. Loosen the cap.02. Melt the medium in water bath at 100 C. Avoid overheating.
03. Cool to 45 C, mix well and pour plates.
Nutrient Agar is used as a general-purpose culture medium for the cultivation of
bacteria, which may also be used as enrichment media by incorporating 10% v/v
sterile blood or other biological fluids.
Directions
1. Loosen the cap.02. Melt the medium in water bath at 100 C.
03. Cool to 45 C and pour plates.
Nutrient Broth are used as general-purpose culture media for the cultivation of
microorganisms.
Direction
1. Use as per requirements.
Use
Peptone Water and Peptone Water with Phenol Red are non-selective media used
for cultivating non-fastidious organisms and as a base for carbohydrate
fermentation media.
Directions
1. Loosen the cap.02. Melt the medium in water bath at 100 C. Avoid overheating.
03. Cool to 45 C and pour plates.
Use
Plate Count Agar (Standard Methods Agar) is used for obtaining microbial plate
counts from milk and dairy products, foods, water and other materials of sanitary
importance.
Directions
1. Loosen the cap.02. Melt the medium in water bath at 100 C. Avoid overheating.
03. Cool to 45 C and pour plates.
Phosphate Buffer USP is used as diluent for raw material and finished product
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SIMMONS CITRATE AGAR
SOYABEAN CASEIN DIGEST AGAR
SS AGAR
TSB + 25% CAP4
TRIPLE SUGAR IRON AGAR
VIOLET RED BILE AGAR
Simmons Citrate Agar is used for the differentiation of gram-negative bacteria on
the basis of citrate utilization.
Directions
1. Loosen the cap.02. Melt the medium in water bath at 100 C.
03. Cool to 45 C and pour tubes.
4. Keep in slanted position for use as slants.
Soyabean Casein Digest Agar is a general-purpose medium used for the isolation
and cultivation of a wide variety of fastidious and non-fastidious microorganisms.
Directions
1. Loosen the cap.02. Melt the medium in water bath at 100 C. Do not overheat.
03. Cool to 45 C and pour plates.
A differential and selective medium for isolation of Salmonella and some Shigella
species from clinical and non-clinical specimens.
Direction
1. Loosen the cap.02. Melt the medium in water bath at 100 C. Avoid overheating.
03. Cool to 45 C and pour plates.
A medium for detection and isolation of bacterial contamination from clean
surface and accidentally contaminated raw material samples of pharmaceutical
and cosmetic formulations.
Triple Sugar Iron Agar is used for the identification of gram-negative enteric
bacilli on the basis of dextrose, lactose and sucrose fermentation and hydrogen
sulphide production.
Directions
1. Loosen the cap.02. Melt the medium in water bath at 100 C.
03. Cool to 45 C and pour tubes.
5. Allow the medium to set in sloped form with a butt about 1 inch long.
Violet Red Bile Agar is a selective medium used for the detection and enumeration
of coliforms.
Directions
1. Loosen the cap.
testing as per US pharmacopoeia.
Plate Count Agar (Standard Methods Agar) is used for obtaining microbial plate
counts from milk and dairy products, foods, water and other materials of sanitary
importance.
Directions
1. Loosen the cap.02. Melt the medium in water bath at 100 C.
03. Cool to 45 C and pour plates.
Potato Dextrose Agar is used for the cultivation and enumeration of yeasts and
moulds from dairy and other food products.
Directions
1. Loosen the cap.02. Melt the medium in water bath at 100 C.
3. When required to alter the reaction of the medium to pH 3.5, cool the base to 045 C and aseptically add an appropriate amount of sterile 10% tartaric acid
(approximately 1ml in 100 ml of medium) and mix well.04. Cool to 45 C and pour plates.
5. Do not reheat the medium after addition of acid.
A medium recommended for selective enrichment of Salmonella species under
condition of high osmotic pressure and low pH with modest nutritional
requirement.
Direction
1. Use as per requirements.
Sabouraud Dextrose Agar is a general-purpose medium used for the cultivation of
yeasts, moulds and aciduric bacteria.
Directions
1. Loosen the cap.02. Melt the medium in water bath at 100 C.
03. Cool to 45 C and pour plates.
Saline is used as a diluent for raw material and finished product testing.
An enrichment medium for the isolation of Salmonella species from faeces, urine,
water, foods and other material of sanitary importance.
Direction
1. Use as per requirements.
PLATE COUNT AGAR
POTATO DEXTROSE AGAR
RAPPAPORT VASSILIADIS SALMONELLA ENRICHMENT BROTH USP
SABOURAUD DEXTROSE AGAR
SALINE
SELENITE BROTH
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Blood Culture System is a fast, efficient and simpler detection system for
preliminary identification of microorganisms from blood.
The indications for obtaining blood cultures are manifold but are basically the
occurrence of a sudden relative change in pulse rate and temperature, with or
without chills, prostration, and hypotension; a history of mild, intermittent, and
persistent fever in association with a heart murmur; and generally any time sepsis
is suspected.
The technology of blood culture is revived by Gould and Duerden (1983). In most
bacterial infections of the blood in man the organisms are not numerous, and it is
essential for their demonstration by blood culture that a relatively large amount of
blood, e.g. 5-10 ml, should be used as the inoculum. In children and infants the
order of magnitude of bacteremia is considerably greater, so that a small amount
of blood (usually 1-5 ml) can be drawn. As the blood's natural bactericidal or
bacteriostatic action may interfere with the growth of any bacteria present, this
effect should be annulled by diluting the blood with medium. There is evidence
that upto 10 ml of blood, or probably more, may be added to 100 ml of medium
without a detectable antibacterial effect. For practical reasons, it is usual to add
5-7 ml of blood to about 50 ml of medium. An anticoagulant is added to the
medium to avoid clotting of blood, which would entrap bacteria and prevent their
detection. The most commonly used preparation in blood culture media today is
0.025% to 0.05% Sodium Polyanethol Sulfonate (SPS) (liquoid). In addition to
its anticoagulant properties, SPS is also anticomplementary, antiphagocytic, and
interferes with the activity of some antimicrobial agents, notably
aminoglycosides.
Blood culture includes qualitative detection of microorganisms in a patient's
blood. Culture of a patient's blood is one of the most important investigations in
clinical microbiology, for the demonstration of septicaemia or bacteriemia
indicates that there is an immediate threat to the patient's life and an urgent need
for appropriate antibiotic therapy.
Blood culture is requested mainly in two clinical situations: 1) where the
possibility of septicaemia or bacteriaemia is suggested by the presence of fever,
shock or other symptoms occurring in association with a known or suspected local
infection such as sepsis in a surgical wound, puerperal sepsis, pneumonia,
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02. Melt the medium in water bath at 100 C.03. Cool to 45 C and pour into plates containing the inoculum.
XLD Agar is a moderately selective medium used for the isolation and
differentiation of Salmonella and Shigella.
XLD AGAR
Directions
1. Loosen the cap.02. Melt the medium in water bath at 100 C. Do not overheat.
03. Cool to 45 C and pour plates.
Blood Culturing Systems
meningitis, osteomyletis or endocarditis; and 2) where it is one of the procedures
required in the investigation of a fever difficult to diagnose because of the absence
of signs of a specific infection or local infective lesion, i.e. a pyrexia of unknown
origin.
The isolation of a bacterium from the blood of a patient from a local infection such
as wound sepsis or pneumonia is valuable firstly in indicating the urgent need for
antibacterial therapy, secondly in revealing the species of bacterium against
which therapy should be directed, and thirdly in providing a culture for the
performance of in-vitro drug sensitivity tests.CULTURE MEDIUM
It is advisable to seed more than one medium for blood culture. The wide range of
media includes brain heart infusion broth, cooked meat medium, fastidious
anaerobe broth, thioglycollate broth and soyabean casein digest broth.
The chance of detecting scanty or exacting bacteria will be maximized if a variety
of each media are seeded from each sample and different media are used for
aerobes and anaerobes.
CONTAMINATION
A difficulty in interpreting results arises from the liability of the specimen to be
contaminated with organisms that are commensals or common contaminants on
the skin and the finding of such organisms in the blood culture should be viewed
with the strong suspicion that it is a contaminant from the skin. But in a patient
with debility, immune deficiency, an intravascular line, a prosthetic implant or
some other condition favouring an opportunistic infection, the saprophytic or
commensal organism isolated may indeed have an important pathogenic role.
Consideration must be given to the probability of it having such an opportunistic
role in the circumstances of each particular case.
COLLECTION OF SAMPLE
To minimize difficulty in interpretation, every care must be taken to prevent
contamination of the specimen during its collection from the patient and its
examination in the laboratory. Proper procedures must be adopted for collection.
False negative results: The media and methods used for blood culture should be
such as to support growth of nutritionally exacting organisms, and both strict
aerobes and anaerobes as well as facultative organisms. Even so, a single
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negative culture should not be taken as proof that a patient does not have a blood
infection. In bacteriaemic illnesses the number of bacteria in blood may vary
rapidly between many and few or none as they are introduced intermittently from
a local infective focus and removed by the reticuloendothelial tissues. To
maximize the chances of isolation when the bacteria may be scanty, a large
volume of blood, e.g. 10-20 ml, from each collection should be cultured, and a
number of samples should be collected on separate occasions.
ANTIBIOTICS IN THE BLOOD
The blood sample should be diluted in the culture medium in order to reduce the
concentration of natural antibacterial constituents to a sub-effective level. The
dilution also reduces the concentration of any therapeutically administered
antibiotic. For this reason, every attempt should be made to ensure that samples
of blood are collected before the start of antibiotic therapy. If an antibiotic has
already been administered, the collection of the blood sample should preferably
be delayed until at least 24 hours after a dose has last been given. Otherwise, it
may be possible to add an antibiotic-inactivating agent to the culture bottle.
CULTURE SYSTEMS
Decisions have to be made about 1) the kinds and numbers of culture media to be
seeded from each sample of bottle, 2) the methods of incubation of the culture
bottles in the laboratory, 3) the number and times of examinations of the bottles
during their incubation, and the methods of examination by filming and
subculture.
CULTURE BOTTLES
It is necessary to culture at least several milliliters of blood diluted 1 in 5 to 1 in 10
in a culture medium. To maximize the chances of detecting scanty bacteria, a
larger volume, e.g. 10-20 ml of blood should be collected and half of it should be
inoculated into each of a 'set' of two such culture bottles. The use of two bottles has
several further advantages: they may contain different media or may be
incubated in different atmospheres, and scanty contaminants are likely to grow in
only one of them. The blood is inoculated into the bottle by inserting the syringe
needle through the rubber washer, the bottle remaining unopened and
unexposed to the entry of airborne dust.EXAMINATION OF BLOOD CULTURE
Depending on the number of bacteria in the blood stream and other factors, the
first appearance of growth may take place after as little as a few hours or only
after a longer period of 1 or a few days, or occasionally one or two weeks. As it is
desired to detect any positive culture as early as possible and yet not miss any late
growing culture, the ideal procedure is to make repeated examinations of the
culture bottle at different times during its incubation from 4 hours to two weeks or
longer.
Trials of different schedules suggest that a minimal, reasonably effective
procedure is the making of just two examinations on each bottle, the first after 0incubation at 37 C overnight, the second after 4-7 days. Only very few cultures
negative at 4 days become positive on longer incubation. When a report is required
urgently on a suspected septicaemia, an additional examination may be made
after only 4 hours incubation.
Some workers, however, consider that an examination should always be made on
the day the specimen is received, i.e. after incubation for only 4-8 hours. Positive
findings at that stage are available for earlier reporting to the clinician and are less
likely to be due to the growth of scanty contaminants than those obtained after
longer incubation. Also, the negative cultures, have to be examined again at 18-
24 hours and 4-7 days, requiring a total of three examinations.
SUBCULTURES
It is recommended that on each subsequent occasion of examination, the blood
culture should be examined both by a gram film and by subculturing on two plates
of blood agar, the one incubated in air, plus 5-10% CO , the other anaerobically in 2
nitrogen/hydrogen plus 5-10% CO . If the presence of meningococcus or 2
haemophilus seems likely, e.g. in suspected septicaemic meningitis, a heated
blood agar (chocolate agar) plate may be substituted for the aerobic plain blood
agar plate. Gram filming is particularly valuable when the first examination is
made only after overnight incubation, i.e. after 12-24 hours, for when bacteria are
seen to be present in the film, a preliminary report may be given to the physician at
once.
We offer blood culture media bottles to meet the wide range of blood culture
demands that are put on hospitals and reference labs. These help to improve
patient diagnosis and treatments and reduce health care costs by optimizing
septicemia diagnosis and maximizing lab efficiency, safety and productivity. These
support the recovery of bacteria and fungi present in blood.
For procedures, storage, directions for use, references, results and other
information, refer to the package insert of individual product.
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BHI With 0.05% SPSSoyabean Casein Digest Broth With 0.05% SPS
Summary and Principle
The occurrence of a sudden relative change in pulse rate and temperature with or
without chills, hyperventilation are indications of suspected septicemia.
Septicemia in hospital patients has increased largely, with a corresponding
increase in morbidity and mortality. Hence, for cases of suspected septicemia, the
culturing of blood for determination of bacteria and fungi becomes mandatory.
Blood culture bottles are used primarily for culturing blood to detect aerobic,
facultative, or anaerobic microorganisms present in the blood stream. The
medium contains all essential nutrients to allow the recovery of microorganisms
present. Blood is withdrawn from the patient using a sterile needle and added to
the blood culturing bottle containing 0.05% Sodium Polyanethol Sulfonate
(SPS). SPS is a polyanionic anticoagulant which inhibits complement and
lysozyme activity, interferes with phagocytosis and inactivates antibiotics,
particularly aminoglycosides. After appropriate incubation, the bottle is observed
for turbidity, colour change, hemolysis, gas formation and other evidence of
growth.
Reagent
BHI WITH 0.05% SPS
INGREDIENTS GRAMS/LITRE
Calf brain Infusion 9.0
Beef Infusion 9.0
Proteose Peptone 10.0
Dextrose 2.0
Sodium Chloride 5.0
Di-Sodium Phosphate 2.0
SPS 0.5
pH: 7.4±0.2
SOYABEAN CASEIN DIGEST BROTH WITH 0.05% SPS
INGREDIENTS GRAMS/LITRE
Tryptone 15.0
Soytone 5.0
Sodium Chloride 5.0
Dipotassium Phosphate 2.5
Dextrose 2.5
SPS 0.5
pH:7.3 ±0.2
GLUCOSE BROTH – SUPPLEMENTED WITH 0.05% SPS
INGREDIENTS GRAMS/LITRE
Glucose 5.0
Tryptone 10.0
Sodium Chloride 5.0
SPS 0.5
pH: 7.3 + 0.2
Interpretation of Results
The medium allows good recovery of microorganisms present which can be further
identified by Gram stain and subculturing onto appropriate medium. Growth in
the broth medium is indicated by the presence of turbidity.
Mycobacteriology
The genus Mycobacterium are 'acid fast bacilli', which are difficult to stain, but
once stained resist decolourization with acid and alcohol. They include the
organisms of human tuberculosis, M. tuberculosis, bovine tuberculosis, M.bovis,
and avium tuberculosis, M.avium, and that of leprosy, M.leprae, which is not fully
acid- and alcohol-fast. The commonest species are classified in two groups: 1) the
typical tubercle bacilli, M.tuberculosis and M.bovis, and their variants, and 2)
the atypical mycobacteria, comprising most members of the genus mycobacteria
including commensal, saprophytic and opportunistically pathogenic species
(photochromogens, scotochromogens, non-chromogens, rapid growers). They
are commonly referred to as tuberculoid bacilli or mycobacteria other than
tubercle (MOTT).
Of particular importance are the tubercle bacilli, mainly M.tuberculosis, being
responsible for over 90% of all cases of tuberculosis, is almost exclusively a
parasite of humans and has mainly an airborne mode of transmission. Infection
with Mycobacterium tuberculosis remains a major public health problem.
Although effective methods for its control are available and have been applied
successfully for several decades, in some regions the prevalence of tuberculosis is
still inordinately high and remains the leading cause of death among notifiable
infectious diseases.
Atypical mycobacteria (tuberculoid bacilli or MOTT) mainly being commensals
and saprophytes may cause opportunistic infections in man indistinguishable
clinically, radiologically and histologically from that caused by the human
tubercle bacillus. Such infection is called mycobacteriosis, is not notifiable as
tuberculosis and generally there is no person-to-person transmission.
Culture, isolation and sensitivity of M.tuberculosis from patient groups using
standard methods remain the gold standard for its detection and effective
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Glucose Broth – Supplemented With 0.05% Sps
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treatment worldwide. Differentiation of M.tuberculosis is possible on the basis of
rate and temperature of growth, cultural characteristics and morphology. Also,
various biochemical and other tests have been used to differentiate tubercle
bacilli from mycobacteria other than tubercle (MOTT) and also to differentiate
between M.tuberculosis and M.bovis (niacin and catalase reactions, nitrate
activity, pigment production, TCH and PNB sensitivity, drug susceptibility
patterns, etc). The identification of mycobacteria to the species level is important
because of its clinical significance, as also in order to provide adequate disease
management because specific antimycobacterial drugs are required against
different pathogenic species of mycobacteria.We offer a range of products for mycobacteriology as listed below. For procedures,
storage, references, results and other information, refer to the package insert of
individual product.
Ready to use...
Summary and Principle
Mycocult is a ready to use Lowenstein-Jensen solid medium for M. tuberculosis
isolation
Lowenstein-Jensen medium supports good growth of M.tuberculosis. It contains
all essential nutrients required for the cultivation of M.tuberculosis. Also, glycerol
present in the formulation enhances the growth of M.tuberculosis. Malachite
green present in optimum concentration not only inhibits contaminating
microflora, but also provides the desired colour contrast for easy identification of
M.tuberculosis colonies.
MycocultReagent
Mycocult LJ medium is provided as a ready to use slant. It is a standard non-
selective inspissated egg based solid medium for the isolation of M.tuberculosis
from specimens such as sputum, CSF and urine.
Interpretation of Results
M. tuberculosis colonies may be detected from third week onwards upto eight
weeks. The colonies are characterized by granular buff coloured growth, which
have an initial size of 1-3 mm and full grown size of 5-8 mm.
Mycocult PYSummary and Principle
Mycocult PY is a ready to use solid medium for the isolation of M.bovis. L.J.
medium contains all essential nutrients required for the cultivation of M.bovis.
Also, sodium pyruvate present in the formulation enhances the growth of M.bovis.
Malachite green present in optimum concentration not only inhibits
contaminating microflora, but also provides the desired colour contrast for easy
identification of M.bovis colonies.
Reagent
Lowenstein-Jensen medium is provided as a ready to use slant. It is a standard
non-selective inspissated egg based solid medium containing sodium pyruvate
for the isolation of M.bovis from specimens such as sputum, CSF and urine.
Interpretation of Results
M.bovis colonies can be detected from third week onwards up to eight weeks. The
colonies are characterized by tiny transparent growth which later becomes white.
Summary and Principle
The genus Mycobacterium are acid-fast bacilli, which are difficult to stain, but
once stained resist decolourization with acid and alcohol. Mycostain is an acid fast
stain for screening of M.tuberculosis and M.leprae.
Mycostain is a standard Ziehl Neelsen hot stain set for the screening of
M.tuberculosis from specimens such as sputum, CSF, urine and isolated culture. It
is also used in the identification of M.leprae from ulcerated nodules on skin and
ear lobe, scrapings and secretions from nasal mucosa and sputum specimens.
Carbol fuchsin forms acid insoluble complex with the mycolic acid present in the
cell walls of the acid- fast bacilli and renders red/pinkish red colour to
Mycostain
M.tuberculosis or M.leprae. M.tuberculosis being acid fast in nature, resists
decolourization by strong acid. Other elements present in the smear are
decolourized with sulphuric acid and hence take up the counter stain (methylene
blue) and are stained blue.Reagent
Mycostain comprises of
a) Mycostain A-Ready to use carbol fuchsin.
b) Mycostain B-Ready to use Loeffler's methylene blue.
Interpretation of Results
a) Acid-fast bacilli stain pink to red colour.
b) Pus cells and other contaminants stain purple to blue colour.
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lle
ry
“If you want to be a winner you have to be willing to give it your all.”
Dehydrated Culture Media ■ Bases ■ Supplements
Bacteroides Bile Esculin Agar Baird Parker Agar
Uninoculated Plate
Bismuth Sulphite Agar
Uninoculated Plate
Bordet Gengou Agar Base
Candida albicansATCC 10231
Brain Heart Infusion Agar
Uninoculated Plate
Brilliant Green Agar Modified
Salmonella typhiATCC 19430
Bordetella pertussisATCC 8467
Bordetella parapertussisATCC 15311
Salmonella typhimuriumATCC 14028
Bacteroides fragilisATCC 25285
Uninoculated Plate Staphylococcus aureusATCC 25923
Salmonella typhimuriumATCC 14028
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Brilliant Green Bile Agar Cetrimide Agar Base
Columbia Blood Agar Base Columbia Blood Agar Base
Uninoculated Plate
Dexoycholate Citrate Agar
Uninoculated Plate
Dextrose Tryptone Agar
Staphylococcus aureusATCC 25923
Bacillus coagulansATCC 7050
Uninoculated Plate Uninoculated Plate Pseudomonas aeruginosaATCC 27853
Enterobacter aerogenesATCC 13048
Escherichia coliATCC 25922
Salmonella enteritidisATCC 13076
Escherichia coliATCC 25922
Streptococcus pyogenesATCC 19615
StreptococcuspneumoniaeATCC 6305
Staphylococcus aureusATCC 25923
Salmonella typhimuriumATCC 14028
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EMB Agar, Levine Lactobacillus MRS Agar
Escherichia coliATCC 25922
MacConkey Agar
Uninoculated Plate
MacConkey Agar w/o CV
Uninoculated Plate
Malt Agar
Uninoculated Plate
Mannitol Salt Agar
Proteus mirabilisATCC 12453
Escherichia coliATCC 25922
Staphylococcus epidermidisATCC 12228
Escherichia coliATCC 25922
Lactobacillus delbrueckii subsp. lactisATCC 7830
Aspergillus nigerATCC 16404
Candida albicansATCC 10231
Saccharomyces cerevisiaeATCC 9763
Staphylococcus aureusATCC 25923
Proteus mirabilisATCC 12453
Salmonella typhimuriumATCC 14028
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Potato Dextrose Agar Pseudomonas Isolation Agar
Sabouraud Dextrose Agar Soyabean Casein Digest Agar
Shigella flexneriATCC 12022
SS Agar
Vibrio choleraeATCC 14033
TCBS Agar
Candida albicansATCC 10231
Uninoculated Plate Pseudomonas aeruginosaATCC 27853
Aspergillus nigerATCC 16404
Candida albicansATCC 10231
Salmonella typhimuriumATCC 14028
Saccharomyces cerevisiaeATCC 9763
Trichophyton mentajrophytesATCC 9533
Staphylococcus aureusATCC 25923
Streptococcus pneumoniaeATCC 6305
Streptococcus pyogenesATCC 19615
Escherichia coliATCC 25922
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Tomato Juice Agar Violet Red Bile Agar
Uninoculated Plate
Vogel & Johnson Agar
Uninoculated Plate
WL Nutrient Agar
Salmonella typhimuriumATCC 14028
XLD Agar
Yersinia enterocoliticaATCC 9610
Yersinia Selective Agar
Staphylococcus aureusATCC 25923
Escherichia coliATCC 25922
Uninoculated Plate Uninoculated Plate Enterobacter aerogenesATCC 13048
Lactobacillus rhamnosusATCC 9595
Escherichia coliATCC 25922
Staphylococcus aureusATCC 25923with Potassium Tellurite
Saccharomyces cerevisiaeATCC 9763
Shigella flexneriATCC 12022
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Brain Heart Infusion Broth Brilliant Green Bile Broth 2%
Kligler Iron Agar Lactose Broth
Simmons Citrate Agar Triple Sugar Iron Agar
UninoculatedTube
Escherichia coliATCC 25922
Uninoculated Tube
Escherichia coliATCC 25922
Uninoculated Tube
Streptococcus pyogenesATCC 19615
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Neisseria meningitidisATCC 13090
Salmonella typhimuriumATCC 14028
Escherichia coliATCC 25922
Enterobacter aerogenesATCC 13048
Uninoculated Tube
Escherichia coliATCC 25922
Shigella flexneriATCC 12022
UninoculatedTube
Escherichia coliATCC 25922
Uninoculated Tube
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MicroxpressReady to use...
In many cases scientists and health professionals need to identify a microbe in a
given environment. Learning the members of a population of microbes present in
nature can give us new insights into biochemical processes that are taking place.
This leads to better understanding of environments and the role microbes play in
them. Identifying a microbe growing in a patient will help identify the disease
caused and thereby the treatments that are effective at eliminating it.
Identification tests can also be used to monitor and eliminate microorganisms
present in the food supply, thereby increasing food safety by eliminating
pathogens and decreasing spoilage.
Bacteria of different genera can differ greatly in their morphological
characteristics. These differences can help us in identifying different bacteria.
Likewise, some bacterial species differ in their cellular morphologies and staining
properties. Again, these differences can be used to aid in identifying bacterial
species. Generally selective and/or differential media are used to aid in
identifying bacterial species. However, many bacteria may share the same
morphological and cultural characteristics-responses to differential staining, size
and shape of the individual cells, size and shape of the colonies, the growth rate in
the presence or absence of oxygen, at different temperatures, in varied pH ranges,
and many others-so these features are rarely sufficient to classify an organism.
Various strains, species and genera of organisms exhibit characteristic patterns of
substrate utilization, metabolic product formation and sugar fermentation. To
determine the identification of a bacterium, it is necessary to study its
metabolism, characterizing the distinctive biochemical reactions that take place
within a cell. The type of biochemical reaction each organism undergoes act as a
“thumbprint” for its identification. Metabolic reactions involve enzymes (bacteria
are among the most diverse organisms with respect to the type of enzyme they
contain), which are proteins that catalyze biochemical reactions and therefore
identification and classification of bacteria is based on the distinctive enzymatic
Microorganism Identification
makeup of bacterial cells.
Carbohydrate utilization studies is an offshoot technology related to biochemical
analysis. All organisms require a carbon source, as carbon is the backbone
material of biological molecules. Most bacteria obtain energy from the oxidation
of organic compounds such as sugars. Strict aerobes oxidize sugars to carbon
dioxide and water through the process of aerobic oxidation while strict anaerobes
perform fermentation reactions. Facultative anaerobes posess the enzymes that
allow them to perform either fermentation or respiration depending on the
availability of oxygen. The types of compounds that an organism can ferment and
the end products that are produced depends on the enzymes the microorganism
can make. Among the end products commonly generated by fermentation are
organic acids and gases such as carbon dioxide and hydrogen. A positive
fermentation reaction is indicated by a change in the colour of the indicator
present in the medium.
When identifying a suspected organism, a series of differential media containing
indicators are inoculated and incubated. One can determine if a given bacterial
species can utilize a given carbohydrate by checking for the presence of
byproducts that are produced by the oxidation of these carbohydrates. pH
indicators present in the media detect metabolic acids that have been produced
by the bacteria after the oxidation and fermentation of sugars giving some form of
visible reaction such as a colour change. Alternatively, reagents may be added to
media after the bacteria have grown; these reagents react with specific byproducts
or intermediates in a metabolic pathway to give a colour change. The results of
these tests on the suspected organism are then compared to known standards to
confirm its identification.
We offer a series of biochemical test kits, which are standardized miniaturized
versions of conventional procedures (biochemical tests).
General Biochemical Identification TestsSUMMARY AND PRINCIPLE
Biochemical tests used for identification of bacteria are most often qualitative.
Media used in these types of test are usually general type media containing
indicators, suitable for the growth of most organisms.
The tests are based on the principle of pH change and substrate utilization
designed to identify various metabolic properties of different bacterial species
that can be used to differentiate even closely related organisms. The kit allows
one to quickly perform approximately 12 tests on a sample. The kits contain
sterile broth for carbohydrate utilization, substrate utilization and other tests for
identification of organisms. The broths are reconstituted by adding a bacterial
suspension. After incubating for an appropriate period, the broths are examined
when the various indicator systems present are affected by the metabolites or
added reagents to give a colour change.
Package insert supplied in the kit allows one to understand the procedure, type of
components that are present and the way they aid in the identification of bacteria.
The system also contains an identification index (easy to read chart), result
interpretation chart and data entry chart that allows one to quickly identify an
unknown isolate. For procedures, storage, references, interpretation of
results, complete list of organisms that can be identified with individual
kit and precautions refer provided package insert of respective kit.
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Candida Identification Kit is a standardized identification system, comprising of
12 miniatured biochemical tests for identification of Candida species. The test
allows identification of the isolate in 24 hours.
Candida Identification Test KitThe kit contains sterile broth for Urease detection and 11 different carbohydrates
for utilization study-Melibiose, Lactose, Maltose, Sucrose, Raffinose, Galactose,
Trehalose, Cellobiose, Inositol, Xylose, Dulcitol.
E.coli Identification Kit is a standardized identification system, comprising of 12
miniatured biochemical tests for identification of E.coli.
Being one of the most important members of the Enterobacteriaceae, E.coli are
gram negative, lactose fermenting organisms and are the commonest cause of
uncomplicated infections of lower urinary tract. They are routinely isolated from
food, feces, water and other clinical samples. The kit contains sterile broth for
E. Coli Identification Test KitMethyl Red test, Voges Proskauer test, Citrate utilization, Indole test,
Glucuronidase, Nitrate reduction, ONPG, Lysine utilization and 4 different
carbohydrates-Glucose, Lactose, Sucrose, Sorbitol.
Reagents supplied with the kit include Barritt Reagent A, Barritt Reagent B and
Creatine for VP test, Methyl Red Indicator for MR test, Nitrite detection agents
(Nitrite Detection Strip and Zinc Dust ) and Kovac's Reagent for Indole test.
Enterobacteriaceae Identification kit is a standardized identification system,
comprising of 12 miniatured biochemical tests for identification of
Enterobacteriaceae.
Basically gram-negative rods, they are the most frequently isolated bacteria from
clinical specimens. The kit can be used for screening pathogenic organisms from
enteric, urine and other relevant specimens.
Enterobacteriaceae Identification Test KitThe kit contains sterile broth for Indole test, Methyl Red test, Voges Proskauer
test, Citrate utilization, and 8 different carbohydrates for utilization study-
Glucose, Adonitol, Arabinose, Lactose, Sorbitol, Mannitol, Rhamnose, Sucrose).
Reagents supplied with the kit include Kovac's Reagent for Indole test, Methyl Red
Indicator for MR test, Barritt Reagent A, Barritt Reagent B, and Creatine for VP
test.
Gram Negative Identification Kit is a standardized identification system,
comprising of 13 miniatured biochemical tests for identification of gram negative
rods.
Gram Negative identification kit can be used for screening of suspected gram-
negative species isolated from feces, urine, blood or any other similar clinical
isolates. The kit can also be used for validation of known strains. The test allows
identification of the isolate in 6-8 hrs, i.e. in a day's working hours.
Gram-Negative Identification Test KitThe kit contains sterile broth for Lysine utilization, Ornithine utilization, Urease
detection, Phenylalanine deamination (TDA), Nitrate reduction, H S production, 2
Citrate utilization oxidase detectionand 5 different carbohydrates for utilization
study-Glucose, Adonitol, Arabinose, Lactose, Sorbitol.
Reagents supplied with the kit include TDA Reagent and Nitrite detection agents
(Nitrite Detection Strip and Zinc Dust).
Listeria Identification Kit is a standardized identification system, comprising of 12
miniatured biochemical tests for identification of Listeria species.
The kit is used for screening food and clinical samples and also for validating
known strains.
The kit contains sterile broth for Esculin hydrolysis, Voges Proskauer test, Nitrate
Listeria Identification Test Kitreduction, Methyl Red test, Catalase and 7 different carbohydrates-Glucose,
Xylose, Lactose, Mannitol, Rhamnose, alpha-Methyl-D-Mannoside, Ribose.
Reagents supplied with the kit include Barritt Reagent A, Barritt Reagent B, and
Creatine for VP test, Methyl Red indicator and Nitrite detection agents (Nitrite
Detection Strip and Zinc Dust).
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Neisseria Identification Kit is a standardized identification system, comprising of
12 miniatured biochemical tests for identification of Neisseria.
Basically gram-negative rods, they inhabit the mucous membranes of humans.
Neisseria Identification Kit can be used for screening pathogenic Neisseria from
nasopharyngeal, urethral, cervical, rectal and pharyngeal specimens.
The kit contains sterile broth for Urease detection, Voges Proskauer test, Oxidase,
Neisseria Identification Test KitCatalase, Nitrate reduction, ONPG, and 6 different carbohydrates-Glucose,
Maltose, Lactose, Sucrose, Fructose, Mannose.
Reagents supplied with the kit include Barritt Reagent A, Barritt Reagent B and
Creatine for VP test, Gordon McLeod Reagent (Oxidase Reagent) and Nitrite
detection agents (Nitrite Detection Strip and Zinc Dust).
Salmonella Identification Kit is a standardized identification system, comprising
of 12 miniatured biochemical tests for identification of salmonellae.
Salmonella identification kit can be used for screening of feaces, urine, blood or
any other similar clinical specimens suspected of containing Salmonella.
The kit contains sterile broth for Methyl Red test, Voges Proskauer test, Citrate
Salmonella Identification Test Kitutilization, Urease detection, H S production, ONPG, Lysine utilization and 5 2
different carbohydrates-Arabinose, Lactose, Maltose, Sorbitol, Dulcitol).
Reagents supplied with the kit include Barritt Reagent A, Barritt Reagent B and
Creatine for VP test and Methyl Red Indicator for MR test.
Staph Identification Kit is a standardized identification system, comprising of 12
miniatured biochemical tests for identification of Staphylococcus.
Staphylococci are gram positive, non motile organisms of major medical
significance. The kit can be used to screen various clinical specimens.
The kit contains sterile broth for Voges Proskauer test, Phosphatase, ONPG,
Staph Identification Test KitArginine utilization, Urease detection, and 7 different carbohydrates for
utilization study- Arabinose, Lactose, Mannitol, Sucrose, Raffinose, Trehalose,
Maltose.
Reagents supplied with the kit include Barritt Reagent A, Barritt Reagent B and
Creatine for VP test.
Strep Identification Kit is a standardized identification system, comprising of 12
miniatured biochemical tests for identification of streptococci.
The kit contains sterile broth for Esculin hydrolysis, Voges Proskauer's test,
Arginine utilization, PYR hydrolysis, ONPG and 7 different carbohydrates for
Strep Identification Test Kitutilization study-Glucose, Arabinose, Sorbitol, Mannitol, Sucrose, Raffinose,
Ribose.
Reagents supplied with the kit include Barritt Reagent A, Barritt Reagent B and
Creatine for VP test and PYR Reagent.
Ready to use...
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No. Test Reagent to be added Principle Original Colour Positive Negativeof Medium Reaction Reaction
BIOCHEMICAL TESTS RESULT INTERPRETATION CHART
1 Indole test 2-3 drops of Kovac's Reagent Detects deamination of tryptophan Colourless Pinkish-Red Colourless
2 Methyl Red test 1-2 drops of Methyl Red Indicator Detects acid production Colourless Red Yellow
3 Voges Proskauer test 1-2 drops of Creatine, 2-3 drops of Detects acetoin production Colourless Pinkish-Red Colourless/Baritt Reagent A, and 1-2 drops of slight copperBaritt Reagent B
4 Citrate utilization - Detects capability of organism to utilize citrate as sole Yellow-green Blue Yellow greencarbon source
5 Glucose utilization - Detects glucose utilization Red Yellow Red /Pink
6 Adonitol utilization - Detects adonitol utilization Red Yellow Red /Pink
7 Arabinose utilization - Detects arabinose utilization Red Yellow Red /Pink
8 Lactose utilization - Detects lactose utilization Red Yellow Red /Pink
9 Sorbitol utilization - Detects sorbitol utilization Red Yellow Red /Pink
10 Mannitol utilization - Detects mannitol utilization Red Yellow Red /Pink
11 Rhamnose utilization - Detects rhamnose utilization Red Yellow Red /Pink
12 Sucrose utilization - Detects sucrose utilization Red Yellow Red /Pink
13 Raffinose utilization - Detects raffinose utilization Red Yellow Red /Pink
14 Trehalose utilization - Detects trehalose utilization Red Yellow Red /Pink
15 Maltose utilization - Detects maltose utilization Red Yellow Red /Pink
16 Ribose utilization - Detects ribose utilization Red Yellow Red /Pink
17 Mellibiose utilization - Detects mellibiose utilization Red Yellow Red /Pink
18 Galactose utilization - Detects galactose utilization Red Yellow Red /Pink
19 Cellulose utilization - Detects cellulose utilization Red Yellow Red /Pink
20 Inositol utilization - Detects inositol utilization Red Yellow Red /Pink
Ready to use... Microxpress
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No. Test Reagent to be added Principle Original Colour Positive Negativeof Medium Reaction Reaction
21 Xylose utilization - Detects xylose utilization Red Yellow Red /Pink
22 Dulcitol utilization - Detects dulcitol utilization Red Yellow Red /Pink
23 Fructose utilization - Detects fructose utilization Red Yellow Red /Pink
24 Mannose utilization - Detects mannose utilization Red Yellow Red /Pink
25 Alpha-methyl-D-mannoside - Detects alpha-methyl-D-mannoside utilization Red Yellow Red /Pinkutilization
26 Lysine decarboxylase - Detects lysine decarboxylation Olive green Purple
27 Ornithine decarboxylase - Detects ornithine decarboxylation Olive green Purple
28 Phenylalanine deamination 2-3 drops of TDA Reagent Detects phenylalanine deamination activity Colourless Green Colourless
29 Arginine utilization Detects arginine utilization Olive green Purple
30 Oxidase detection 1-2 drops of Gordon McLeod Detects oxidase activity Colourless Purplish Colourless(Oxidase) Reagent
31 Catalase detection 3% H O soln. Detects catalase activity Effervescence No 2 2
seen effervescence
32 Urease detection - Detects urease activity Orangish yellow Pink Orangish yellow
33 Nitrate reduction Nitrite Detection Strip and a pinch Detects nitrate reduction Colourless Pink/red/violet Colourlessof Zinc Dust
34 H S production - Detects H S production Orangish Black Orangish2 2
35 ONPG utilization - Detects beta- galactosidase activity Colourless Yellow Colourless
36 Glucuronidase detection - Detects glucuronidase activity Colourless Blue green No fluoresance
37 Alkaline phosphatase 1-2 drops of 40% NaoH Detects phosphatase enzyme Cream to Pink Cream
38 Esculin hydrolysis - Detects esculin hydrolysis Cream-amber Black Cream
39 PYR test 1-2 drops of PYR Reagent Detects pyroglutamate aminopeptidase activity Cream Cherry red Cream
BIOCHEMICAL TESTS RESULT INTERPRETATION CHART
Olive - GreenYellow
Olive - GreenYellow
Olive - GreenYellow
fluoresance
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IDENTIFICATION INDEX FOR SOME IMPORTANT ORGANISMS
MR
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ORGANISMS
KEY: Based on % strains showing reactions following symbols have been assigned from laboratory results and standard references
+ = Positive (more than 90%) - = Negative (more than 90%) v = 11-98% variable result ND = No data W =Weak reaction
Aeromonas hydrophila d d - - - + + - + - d + -
Candida albicans - - - + - + - - + - - +
Candida tropicalis - - - + + v + - + - - v
Citrobacter freundii + - (+) d - (-) d - + - (+) + - d + + (+) + +
Enterobacter aerogenes - + + - + + - - + - - + + + + + + + +
Enterobacter cloacae - + + - - + d - + - + (-) + + + + + +
Escherichia coli + - - + + d - - + - - + - + + + d + +
Escherichia blattae + - d - + + - - + - + - -
Klebsiella pneumoniae subspecies - + + - + - + - + - - + + + + + + + +pneumonia
Listeria monocytogenes + + - + + v - + - + + -
Morganella morganii + - - + - + + + + - + - - - - - - -
Neisseria gonorrhoea - - + + + - - - - - -
Neisseria meningitidis - - + + + - + - - - -
Proteus mirabilis + d d - - + + + + + + - - - - + - -
Proteus vulgaris + - (-) + - - + + + - + + - - - - + - -
+ - - - -
ND
NDND
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Pseudomonas aeruginosa - + + ND - -
Salmonella cholerasuis subspecies + - + - + + - - + - + + - - + + - v + + -cholerasuis
Salmonella enteritidis + - + - + + - - + - + + - - + +
Salmonella serotype typhi + - - - + - - - + - + + - - - + v
Salmonella serotype typhimurium + - + - + - + - + + + v -
Serratia marcesans (-) + + - + + (-) - + - + d - - + + - +
Shigella boydii, Shigella flexneri, Shigella + - - d - - - - + - + - - d d - - +dysenteriae
Shigella sonnei + - - - - + - - + - + - - + - - v +
Staph. aureus + w w + - + + - + + + -
Staph. epidermidis + w + v - + + - - - + -
Strep. pyogenes - + ND ND - ND - - ND + v
Vibrio cholerae ND + + - - - + + - ND - - -
Vibrio vulnificus + + v - v + + - + - v - -
Yersinia enterocolitica + - - d - + (+) - + - + - - + + + - +
ND ND ND ND + ND ND
IDENTIFICATION INDEX FOR SOME IMPORTANT ORGANISMS
MR
TE
ST
VP
TE
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TILI
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IND
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ORGANISMS
KEY: Based on % strains showing reactions following symbols have been assigned from laboratory results and standard references
+ = Positive (more than 90%) - = Negative (more than 90%) v = 11-98% variable result ND = No data W =Weak reaction
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As seen, various strains, species, and genera of microorganisms exhibit
characteristic patterns of substrate utilization, metabolic product formation and
sugar fermentation. Often, biochemical tests are used to identify microorganisms.
The result can be seen by a colour change. Most reactions are based on the
detection of an enzyme with a certain given substrate. Also, methods to detect
certain metabolites by chemical reaction and or complex building techniques are
used. At the end a colour change gives a result which leads to more cognition of
the unknown organism.
Analytical reagents are used for carrying out biochemical tests used to identify
microorganisms.
In general, different bacteria convert dextrose and glucose to pyruvate using
different metabolic pathways. Some of these pathways produce unstable acidic
Readymade Analytical Reagentsproducts which quickly convert to neutral compounds. Some organisms use the
butylene glycol pathway, which produces neutral end products including acetoin
and/or 2,3-butanediol. Other organisms use the mixed acid pathway, which
produces stable acidic end products such as lactic, formic and acetic acid. A
medium like MR-VP Broth is used to determine the pathway a given organism
uses to ferment glucose; to this end reagents like Methyl Red Indicator or Barritt
Reagent A and B are added along with Creatine to detect end products or
intermediates produced by a given fermentation pathway.
We offer microbiologists carefully standardized analytical reagents in dropper
bottles in order to ensure accurate and interpretable results.
For procedures, storage, references, results and other information, refer
to the package insert of individual product.
Summary and Principle
Baritt Reagent A, Barritt Reagent B and Creatine are used to perform the Voges
Proskauer (VP) test.
The VP test detects organisms that utilize the butylene glycol pathway. These
organisms ferment carbohydrates with the production of acetoin (acetyl methyl
carbinol) or its reduction product 2,3-butylene glycol. The substances can be
tested for by a colorimetric reaction between diacetyl (formed during the test by
oxidation of acetoin or 2,3-butylene glycol) and a guanidino group under
alkaline conditions. When Barritt Reagent A (alpha naphtol) and Barritt Reagent
B (KOH) are added to MR-VP Broth along with Creatine (catalyst) that has been
Barritt Reagent A, Barritt Reagent B, Creatineinoculated with an organism that uses butylene glycol pathway, development of
red colour is a positive reaction for formation of a neutral end product (acetoin).
Reagent
Baritt Reagent A comprises of 40% potassium hydroxide.
Baritt Reagent B comprises of 5% alpha naphtol in absolute alcohol.
Creatine comprises of 0.5% Creatine.
Cultural Response
Organism (ATCC) VP test
E.coli (25922) -
K.pneumoneae (23357) +
Summary and Principle
Methyl Red Indicator is used to perform the Methyl Red (MR) test.
The MR test involves adding the pH indicator methyl red to an inoculated tube of
MRVP Broth. If the organism uses the mixed acid fermentation pathway and
produces stable acidic end products, the acids will overcome the buffers in the
medium and produce an acidic environment. When Methyl Red Indicator is
added, if acidic end products are present, the Methyl Red Indicator will remain red
which is a positive test for stable acidic end products.
Methyl Red IndicatorReagent
Methyl Red Indicator comprises of methyl red 0.02%.
Cultural Response
Organism (ATCC) MR test
E.aerogenes (13048) -
E.coli (25922) +
K.pneumoneae (23357) -
The MR and VP tests are generally employed in conjunction to detect specific
breakdown products and are more often used for identification of enteric bacteria.
Summary and Principle
Gordon McLeod Reagent is used for detection of oxidase activity of a bacterial
culture, i.e. to see if the organism is an aerobe.
Gordon McLeod Reagent (Oxidase Reagent)The test depends on the presence in bacteria of certain oxidases that will catalyze
the transport of electrons between electron donors in bacteria and a dye-N,N,N',N
tetramethyl-p-phenylenediamine dihydrochloride (Gordon McLeod Reagent ).
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Oxidases are enzymes that catalyze the reduction of oxygen during respiration.
For example in gram positive bacteria and many gram negative bacteria
cytochrome oxidase performs the final step in electron transport, reducing oxygen
to water. Other bacteria, such as the Enterobacteriaceae, do not reduce oxygen
using this enzyme. The test is a check for the presence of electron transport chain
that is the final phase of aerobic respiration. Normally, oxygen is the final electron
acceptor in this system. In this test, an artificial electron acceptor (Gordon McLeod
Reagent) is used in place of oxygen. This acceptor is a chemical that changes
colour to dark blue/purple when it takes the electron from the last element
(cytochrome oxidase) in the electron transport chain. (the cytochrome c oxidase
present in most gram negative bacteria will oxidize N,N,N',N-tetramethyl-p-
phenylenediamine dihydrochloride to give a dark purple complex).
The test is used for screening species of Neisseria, Aeromonas, Vibrio,
Campylobacter and Pseudomonas, which give positive reactions and for
excluding the Enterobacteriaceae, all species of which give negative reactions.
Reagent
Gordon McLeod Reagent comprises of 0.5% N,N,N',N-tetramethyl-p-
phenylenediamine dihydrochloride.
Cultural Response
Organism (ATCC) O x i d a s e
test
C.jejuni (29428) +
N.gonorrheae (19424) +
E.aerogenes (13048) -
E.coli (25922) -
Summary and Principle
The ability to degrade amino acids to identifiable end products is often used to
differentiate bacteria. Production of indole using pure cultures in tryptophan-
containing media is recommended as an aid in differentiation of bacteria. Kovac's
Reagent is used for performing the Indole test. The test determines the ability of
certain bacteria, primarily Enterobacteriaceae to decompose the amino acid
tryptophan to indole which accumulates in the medium. Indole is then tested for
by a colorimetric reaction with Kovac's Reagent (p-dimethyl-amino-
benzaldehyde) Indole reacts with the aldehyde present in the Kovac's Reagent to
give a reddish-pink colour in the alcohol layer which is a positive test and is
particularly useful in the identification of E.coli. The alcohol layer extracts and
concentrates the red coloured complex.
Reagent
Kovac's Reagent comprises of 5% p-dimethyl-amino-benzaldehyde.
Cultural Response
Organism (ATCC) Indole test
E.aerogenes (13048) -
E.coli (25922) +
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McFarland Standard No. 0.5
McFarland Standard are used as a reference to adjust the turbidity of bacterial
suspensions and in antimicrobial susceptibility testing.
McFarland Standard is turbidity standard incorporating a suspension of barium
sulphate ions or latex particles (particle size is equal to bacterial cell size ) in
buffer, which makes it turbid. Turbidity of bacterial suspension depend on the
number of bacteria present in suspension. Measurement of turbidity
quantifies the amount of bacteria present in bacterial suspension. If we require a
bacterial suspension of specific number of bacteria, we need to match opacity or
turbidity of bacterial suspension with a chosen opacity standard like McFarland.
Turbidity of 0.5 McFarland standard is equal to a suspension, which contains
1.5X10 numbers of bacteria.
McFarland Standard is used in Antibiotic susceptibility test. Antimicrobial
susceptibility test is used to find the effective antimicrobial agent ( drugs /
antibiotics ) for successful treatment of an infection.
McFarland standard used in antimicrobial susceptibility testing for preparation of
microbial suspension of 1.5X108 numbers of bacteria. The performance of
Antimicrobial Sensitivity Disk Test require appropriate amount of bacterial
suspension which can be obtained by optically comparing turbidity of bacterial
suspension with 0.5 McFarland standard. If the bacterial suspension does not
appear to be the same density as the McFarland 0.5, the turbidity can be reduced
by adding sterile saline or increased by adding more microbial inoculum
(Transferring Bacterial Culture).
Reagent
0.5 mL of 1.175% barium chloride dihydrate (BaCl22H2O)
0.69.95 mL 1% sulfuric acid (H2SO4).
The standard can be compared visually to a suspension of bacteria in sterile
saline. If the bacterial suspension is too turbid, it can be diluted with more saline.
If the suspension is not turbid enough, more bacteria can be added.
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Summary and Principle
PYR Reagent is used in conjunction with PYR testing for the detection of L-
pyroglutamyl aminopeptidase found in group A streptococci and group D
enterococci.
The PYR test uses L-pyrrolidonyl-beta-naphthlyamide substrate to detect the
presence of enzyme L-pyroglutamyl-aminopeptidase. Following hydrolysis of the
substrate by the enzyme the resulting beta-naphthylamine produces a cherry red
pigment upon the addition of PYR Reagent (cinnamaldehyde).
PYR ReagentReagent
PYR Reagent comprises of 1% cinnamaldehyde.
Cultural Response
Organism (ATCC) PYR test
E.faecalis (29212) +
S.bovis (27960) -
S.pyogenes (19615) +
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Summary and Principle
Nitrite detection strip and zinc dust are used to perform the nitrate reduction test.
Bacterial species may be differentiated on the basis of their ability to reduce
nitrate to nitrite or nitrogenous gases. Many bacteria have respiratory enzyme
systems that can use nitrate as a terminal electron acceptor forming nitrite.
Bacterial reduction of nitrate results in the production of various end products like
nitrite, nitrogen gas, ammonia, nitrous oxide and hydroxylamine. The nitrate
reduction characteristic of a particular species is more or less constant making it a
useful diagnostic tool.
The ability to reduce nitrate is valuable for differentiating and identifying various
types of bacteria, especially Enterobacteriaceae. Non-fermenters and other
miscellaneous gram negative bacilli vary in their ability to reduce nitrates. Some
members of this group are capable of denitrification which is a reduction of nitrate
to nitrogen gas. For the glucose fermenting gram negative bacilli, the production
of nitrogen gas is an important differential test. The end product of reduction
depends on bacterial species. Reduction of nitrate is generally an anaerobic
respiration process in which an organism derives its oxygen from nitrate.
This is a test for the presence of the enzyme nitrate reductase which causes the
reduction of nitrate, in the presence of a suitable electron donor, to nitrite which
can be tested for by an appropriate colorimetric reaction. The microorganism
under evaluation is inoculated in the medium and after incubation, nitrate
reduction can be determined. Upon addition of nitrite detection strip during the
test, development of pink, deep red or violet colour indicates a positive reaction.
No change in the colour of the strip only indicates that nitrite is not present in the
medium. There are two explanations for these observations:
Nitrite Detection Kit (General Microbiology)
1) The nitrate may not have been reduced and hence the strain is nitrate
negative.
2) The nitrate may have been reduced to nitrite, and further the nitrite has been
completely reduced to nitric oxide, nitrous oxide or nitrogen which will not
react with the reagents on the nitrite detection strip; the strain is nitrate
positive.
Any test medium that gives a negative result for the nitrite detection strip can be
further tested to determine which of the two interpretations is correct. This is done
by adding a small amount of zinc dust to all negative tests. The zinc dust will
catalyze the reduction of nitrate to nitrite. Thus, if the nitrate has not been reduced
by the organisms, i.e. the strain is nitrate negative, it will be reduced by the zinc
dust and the nitrite detection strip will show colour change from colourless to
pink/red/violet. If no colour change in the nitrite reduction strip is observed after
the addition of zinc dust, the organisms have not only reduced nitrate to nitrite,
but have further reduced nitrite to nitrogenous gases; the organisms are nitrate
positive. Almost all Enterobacteriaceae reduce nitrate.
Reagent
Nitrite Detection Kit comprises of
a. Nitrite Detection Strip
b. Zinc Dust
Cultural Response
Organism (ATCC) Nitrate reduction
E.aerogenes (13048) +
E.coli (25922) +
S.serotype typhimurium (14028) +
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Summary and Principle
TDA Reagent (ferric chloride reagent) is used to visualize the phenylalanine
deamination reaction which allows differentiation of enteric bacilli on the basis of
their ability to produce phenylpyruvic acid by oxidative deamination. This reaction
is very useful in the taxonomy of Enterobacteriaceae.
The test allows to differentiate members of the Proteeae from other members of
the Enterobacteriaceae by the ability of organisms in the genera within the
Proteeae to deaminate phenylalanine to phenylpyruvic acid by enzymatic
activity. Proteus, Providencia and Morganella species possess this capability.
Phenylalanine serves as the substrate for enzymes, which are able to deaminate it
TDA Reagentto form phenylpyruvic acid. The addition of TDA Reagent following incubation
results in appearance of deep green color in case of positive reaction or no color
change in case of negative reaction.
Reagent
TDA Reagent comprises of 10% ferric chloride.
Cultural Response
Organism (ATCC) TDA test
E.aerogenes (13048) -
P.alcalifaciens (9886) +
P.vulgaris (13315) +
As bacteria consists of clear protoplasmic matter, differing but slightly in refractive
index from the medium in which they are growing, it is difficult with the ordinary
microscope, except when special methods of illumination are used, to see them in
the unstained condition. Staining, therefore is of primary importance for the
recognition of bacteria.
Stains are used
lTo render microscopic and semitransparent objects visible.
lTo reveal their shape and size.
lTo show the presence of various internal and external structures.
lTo produce specific chemical and physical reactions.
Apart from differentiating and rendering visible the constituents of the cell, stains
will help to identify the organisms and classify to a particular group by their
individual reactions to particular stains. Broadly stains are made up of dyes which
can be classified as natural or synthetic.
The vast majority of staining procedures applied to microorganisms involves the
use of smears on glass slides. The essential steps in the preparation of a fixed,
stained smear are 1) preparation of the film or smear, 2) fixation and
decolourization, and 3) application of one or more staining solutions. The
advantages of these procedures are that the cells are made more clearly visible
after they are coloured and differences between cells of different species and
within the same species can be demonstrated by use of appropriate staining
solutions (differential or selective staining).
A large number of coloured organic compounds (dyes) are available for staining
microorganisms. These compounds are generally rather complex in terms of
Staining Solutionsmolecular structure and on this basis they may be classified into groups such as
triphenylmethane dyes, oxazine dyes and thiazine dyes.
A more practical classification for the cytologist is one based on the chemical
behaviour of the dye; namely acidic, basic or neutral i.e. the most commonly used
stains are salts composed of a base and an acid. An acid (or anionic) dye is the one
in which the charge on the dye ion is negative; a basic (or cationic) dye is one in
which the charge carried by the dye ion is positive. A neutral dye is a complex salt
of a dye acid with a dye base. Acid dyes generally stain basic cell components, and
basic dyes generally stain acidic cell components. (for eg. protoplasm of the
organism being acidic and negatively charged, will combine with the positively
charged cations or coloured part of the basic dye). The process of staining may
involve ion-exchange reactions between the stain and active sites at the surface of
or within the cell.
Many different kinds of staining solutions are employed in various bacteriological
procedures. Simple staining is carried out by applying a single solution of stain to
the fixed smear wherein the organisms are given the colour characteristic of the
staining solution. These stains bring out the morphology best. Differential stains
utilize more than one stain and make visible the differences between bacterial cell
or parts of a bacterial cell. These stains are necessary to bring out characteristics
like flagella, capsules, spores, metachromatic granules, polyssacharides and
lipids.
We offer microbiologists carefully standardized and reproducible staining
solutions in dropper bottles in order to ensure accurate and interpretable results.
For procedures, storage, references, results and other information, refer
to the package insert of the individual product.
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Summary and Principle
Gram Stain is used to differentiate between gram-positive and gram-negative
bacteria depending on their ability to take up either the primary stain or the
counter stain.
Various mechanisms have been proposed to explain the gram reaction. Most
researchers who have studied the mechanism of gram stain reaction have
concluded that the mechanism is based on the distinctive chemistry and physical
structure of the cell walls of the organisms (there are many physiological
differences between the cell walls of gram-positive and gram-negative bacteria).
Certain bacteria when treated with one of the basic para-rosanaline dye such as
crystal violet (primary stain), and then iodine, 'fix' the stain so that subsequent
treatment with a decolourizing agent like alcohol does not remove the colour.
Other organisms, however, are decoulorized by this process. If a mixture of various
organisms are thus stained and subjected to the decolourizer, some organisms
retain the dye and turn violet in colour-these are termed as gram positive,
whereas others that are completely decoulorized are termed gram negative. In
order to render the decoulorized organisms visible, and to distinguish them from
those retaining the colour, a counter stain like methyl red or safranine is applied.
These organisms take up the stain methyl red and turn red in colour.
Generally, a thin smear of bacterial cell suspension is stained with crystal violet,
then treated with an iodine containing mordant to increase the binding of the
primary stain. A decolourizing solution of alcohol is used to remove the crystal
violet from cells which bind it weakly and then a counterstain like safranin is used
to provide a colour contrast in those cells that are decolourized.
The gram positive characteristic results from the ability of a cell to resist the loss of
the primary stain during the decolourization step, as compared to the gram
negative cells which do not possess this ability. The explanation of this
phenomenon is associated with the composition and structure of the cell wall.
Gram Stain KitThe gram-positive organisms have more mucopeptide in their cell walls as
compared to gram-negative ones. On the other hand, gram-negative bacteria
have a high content of lipids in their cell walls. Cell walls of gram-positive
organisms also contain teichoic acids in abundance, not seen in cell walls of
gram-negative organisms. Experimental evidence suggest that during staining of
gram negative bacteria, the alcohol treatment extracts the lipids, which results in
increased porosity or permeability of the cell wall. Thus the crystal violet-iodine
(CV-I) complex can be extracted and the gram negative organism is decolourized,
which subsequently take up the colour of the counter stain. The gram positive
bacteria, because of their lower lipid content become dehydrated during alcohol
treatment. The pore size is decreased, permeability is reduced and the CV-I
complex cannot be extracted. Therefore these cells remain purple violet and do not
take up the counterstain.
The gram stain has its greatest use in characterizing bacteria.
Reagent
Gram Stain Kit is provided as a four component reagent.
Gram’s Crystal Violet solution (Reagent A)
2% crystal violet.
Gram’s Iodine-Iodide solution (Reagent B)
0.66% potassium iodide and 0.33% iodine
Games Decolorizer solution (Reagent C )
50% Acetone in 95% Ethanol.Gram’s Safranine solution (Reagent D)
0.25% safranine.
Observation
Gram positive organisms stain purple in colour whereas gram negative organisms
stain red in colour.
Lactophenol Cotton BlueSummary and Principle
Lactophenol Cotton Blue wet mount preparation is the most widely used method
of staining and observing fungi. It is formulated with lactophenol, which serves as
the mounting fluid and the dye, cotton blue. Lactic acid preserves the fungal
structure and clears the tissue while phenol acts as a disinfectant. The high
concentration of phenol deactivates lytic cellular enzymes thus the cells do not
lyse. Cotton blue is an acid dye that stains the chitin present in the cell walls. The
stain imparts blue colour on fungal hyphae.
Reagent
Lactophenol Cotton Blue comprises of
20% phenol, 0.05% cotton blue, 20% lactic acid in glycerol
Phenol 20 gms
Cotton blue 0.05 gms
Lactic acid 20 ml
Glycerol 40 ml
Distilled water 100 ml
Observation
Fungal hyphae stain blue in colour.
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Summary and Principle
Lactophenol Picric Acid is used for staining as well as for wet mounting of fungi.
Picric acid is a nitrophenol.
Reagent
Lactophenol Picric Acid comprises of
Phenol 100 gms
Lactophenol Picric AcidLactic acid 100 ml
Glycerol 200 ml
Picric acid, saturated aqueous solution 100 ml
Observation
Fungal spores and hyphae stain pale to dark yellow.
Summary and Principle
Picric acid is used for staining of fungi. It is a 2,4,6-trinitrophenol having
antibacterial as well as antifungal properties and stains the fungal spores pale to
dark yellow.
Reagent
Picric Acid comprises of
Picric AcidPicric acid 1.18 gms
Distilled water 100 ml
Observation
Fungal spores stain pale to dark yellow.
425
APPLICATIONS
Food and Beverage Analysis
PRODUCTS
Bac
illus
cer
eus
Aci
duric
and
The
rmop
hilic
Fl
at S
our S
pore
form
ers
Cam
pylo
bact
erC
lost
ridiu
m
Col
iform
s
List
eria
Mes
ophi
lic S
pore
form
ers
(Aer
obic
)
Osm
ophi
lic o
rgan
ism
s
Psy
chro
troph
ic O
rgan
ism
sS
alm
onel
la
Shi
gella
Sta
phyl
ococ
cus
Stre
ptoc
occu
s/E
nter
ococ
cus
Tota
l Pla
te C
ount
Vib
rio
Yeas
ts a
nd M
ould
sYe
rsin
ia e
nter
ocol
itica
Mes
ophi
lic S
pore
form
ers
(Ana
erob
ic)
Iron
Sul
phite
spo
refo
rmer
s
Pro
teol
ytic
mic
roor
gani
sms
Alkaline Peptone water
Azide Dextrose Broth (AM50061)
Bacillus Cereus Agar Base (AM1009/AM5009)
Baird Parker Agar Base (AM1011/AM5011)
BP Sulpha Supplement* (AS005)
Potassium Tellurite 3.5%* (AS023)
Bile Esculin Agar (AM1012/AM5012)
Bismuth Sulphite Agar (AM1013/AM5013)
Blood Agar Base (AM1014/AM5014)
Brain Heart Infusion Agar (AM1016/AM5016)
Brain Heart Infusion Broth (AM1017/AM5017)
Brilliant Green Agar, Modified (AM1018/AM5018)
Sulpha Supplement* (AS027)
Brilliant Green Bile Broth 2% (AM1020/AM5020)
Chloramphenicol Yeast Glucose Agar*
Colombia Blood Agar Base (AM1029/AM5029)
Staph-Strepto Supplement* (AS025)
Strepto Supplement* (AS026)
Campylobacter agar Base (AM50218)
Cooked Meat Medium (AM1030/AM5030)
DNase Test Agar Base (AM10381)
Deoxycholate Citrate Agar (AM1031/AM5031)
Dextrose Tryptone Agar (AM1036/AM5036)
Dextrose Tryptone Broth (AM1037/AM5037)
(AM1910/AM5911)
(AM1024/AM5024)
l
l
l
l
l
l
l
l
l l l ll
l
l
l
l
l
l l
l
l
l l
l
Aci
d P
rodu
cing
Bic
robi
olog
y
Aer
ogen
e
Lact
obac
illus
Ent
eroc
occu
s fa
ecal
is
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits 426
Bac
illus
cer
eus
Aci
duric
and
The
rmop
hilic
Fl
at S
our S
pore
form
ers
Cam
pylo
bact
erC
lost
ridiu
m
Col
iform
s
List
eria
Mes
ophi
lic S
pore
form
ers
(Aer
obic
)
Osm
ophi
lic o
rgan
ism
s
Psy
chro
troph
ic O
rgan
ism
sS
alm
onel
la
Shi
gella
Sta
phyl
ococ
cus
Stre
ptoc
occu
s/E
nter
ococ
cus
Tota
l Pla
te C
ount
Vib
rio
Yeas
ts a
nd M
ould
sYe
rsin
ia e
nter
ocol
itica
Mes
ophi
lic S
pore
form
ers
(Ana
erob
ic)
Iron
Sul
phite
spo
refo
rmer
s
Pro
teol
ytic
mic
roor
gani
sms
Aci
d P
rodu
cing
Bic
robi
olog
y
Aer
ogen
e
Lact
obac
illus
Ent
eroc
occu
s fa
ecal
is
APPLICATIONS
Food and Beverage Analysis
PRODUCTSDifferential Reinforced Clostridial Broth (AM1038/AM5038)
EC Broth (AM1039/AM5039)
Fraser Broth Base (AM50456)
(AM104821/AM504821)
(AM50532)
(AM10576/AM50576)
(AM1058/AM5058)
l
l
l l
l
ll
l
l
l l
l l
l
l
l
l l l
l
l
l
l
l
l
l
l ll l
EMB Agar,Levine (AM1040/AM5040)
Fluid Lactose Medium (AM1042/AM5042)
Fluid Sabouraud Medium (AM1043/AM5043)
Fluid Selenite Cystine Medium (AM1044/AM5044)
Fluid Thioglycollate Medium (AM1045/AM5045)
Heart Infusion Agar (AM10481/AM50481)
Heart Infusion Broth (AM10482/AM50482)
Iron Sulphite Agar (AM10483/AM50483)
Karmali Campylobacter Agar Base (AM1049/AM5049)
Campylobacter Selective Supplement with Hemin (Karmali), Modified* (AS007)
Kligler Iron Agar (AM1050/AM5050)
Lactobacilli MRS Agar (AM1051/AM5051)
Lactose Broth (AM1052/AM5052)
Lauryl Tryptose Broth (AM1053/AM5053)
Listeria Identification Agar Base, PALCAM (AM1055/AM5055)
Listeria Selective Supplement *(AS018)
LPM Agar Base (AM10575)
Lysine Iron Agar
MacConkey Agar with Crystal Violet, NaCl and 0.15% Bile Salts
Hektoen Enteric Agar
Lee's Agar
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits427
Bac
illus
cer
eus
Aci
duric
and
The
rmop
hilic
Fl
at S
our S
pore
form
ers
Cam
pylo
bact
erC
lost
ridiu
m
Col
iform
s
List
eria
Mes
ophi
lic S
pore
form
ers
(Aer
obic
)
Osm
ophi
lic o
rgan
ism
s
Psy
chro
troph
ic O
rgan
ism
sS
alm
onel
la
Shi
gella
Sta
phyl
ococ
cus
Stre
ptoc
occu
s/E
nter
ococ
cus
Tota
l Pla
te C
ount
Vib
rio
Yeas
ts a
nd M
ould
sYe
rsin
ia e
nter
ocol
itica
Mes
ophi
lic S
pore
form
ers
(Ana
erob
ic)
Iron
Sul
phite
spo
refo
rmer
s
Pro
teol
ytic
mic
roor
gani
sms
Aci
d P
rodu
cing
Bic
robi
olog
y
Aer
ogen
e
Lact
obac
illus
Ent
eroc
occu
s fa
ecal
is
APPLICATIONS
Food and Beverage Analysis
PRODUCTSDifferential Reinforced Clostridial Broth (AM1038/AM5038)
EC Broth (AM1039/AM5039)
Fraser Broth Base (AM50456)
(AM104821/AM504821)
(AM50532)
(AM10576/AM50576)
(AM1058/AM5058)
l
l
l l
l
ll
l
l
l l
l l
l
l
l
l l l
l
l
l
l
l
l
l
l ll l
EMB Agar,Levine (AM1040/AM5040)
Fluid Lactose Medium (AM1042/AM5042)
Fluid Sabouraud Medium (AM1043/AM5043)
Fluid Selenite Cystine Medium (AM1044/AM5044)
Fluid Thioglycollate Medium (AM1045/AM5045)
Heart Infusion Agar (AM10481/AM50481)
Heart Infusion Broth (AM10482/AM50482)
Iron Sulphite Agar (AM10483/AM50483)
Karmali Campylobacter Agar Base (AM1049/AM5049)
Campylobacter Selective Supplement with Hemin (Karmali), Modified* (AS007)
Kligler Iron Agar (AM1050/AM5050)
Lactobacilli MRS Agar (AM1051/AM5051)
Lactose Broth (AM1052/AM5052)
Lauryl Tryptose Broth (AM1053/AM5053)
Listeria Identification Agar Base, PALCAM (AM1055/AM5055)
Listeria Selective Supplement *(AS018)
LPM Agar Base (AM10575)
Lysine Iron Agar
MacConkey Agar with Crystal Violet, NaCl and 0.15% Bile Salts
Hektoen Enteric Agar
Lee's Agar
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits 428
Bac
illus
cer
eus
Aci
duric
and
The
rmop
hilic
Fl
at S
our S
pore
form
ers
Cam
pylo
bact
erC
lost
ridiu
m
Col
iform
s
List
eria
Mes
ophi
lic S
pore
form
ers
(Aer
obic
)
Osm
ophi
lic o
rgan
ism
s
Psy
chro
troph
ic O
rgan
ism
sS
alm
onel
la
Shi
gella
Sta
phyl
ococ
cus
Stre
ptoc
occu
s/E
nter
ococ
cus
Tota
l Pla
te C
ount
Vib
rio
Yeas
ts a
nd M
ould
sYe
rsin
ia e
nter
ocol
itica
Mes
ophi
lic S
pore
form
ers
(Ana
erob
ic)
Iron
Sul
phite
spo
refo
rmer
s
Pro
teol
ytic
mic
roor
gani
sms
Aci
d P
rodu
cing
Bic
robi
olog
y
Aer
ogen
e
Lact
obac
illus
Ent
eroc
occu
s fa
ecal
is
APPLICATIONS
Food and Beverage Analysis
PRODUCTSMacConkey Broth with Neutral Red (AM1064/AM5064)
MacConkey Broth Purple with Bromocresol Purple (AM1063/AM5063)
(AM10635/AM50635)
MacConkey Broth Harmonized (AM5063)
MacConkey Broth USP (AM50636)
Malonate Broth Ewing Modified (AM1065/AM5065)
Malt Agar (AM1066/AM5066)
Malt Extract Agar (AM1067/AM5067)
Malt Extract Broth (AM1068/AM5068)
Mannitol Motility Test Medium (AM10681/AM50681)
Modified Rappaport Vassiliadis Medium(AM506928)
Orange Serum Broth (AM50783)
l
l
l
l l
l
l
l ll
l
l l
l
l
l
l
l
l
l l l ll l
l
l ll
l
l l ll
l
l
MacConkey Broth Purple with BCP ISO/BIS/BP/IP
MacConkey Broth
Modified Rappaport Vassiliadis Medium for Water
Testing BIS/ISO (AM506929/30)
MUG Lauryl Sulphate Broth (AM50721)
Mannitol Salt Agar (AM1069/AM5069)
Motility Test Medium (AM506933)
MR-VP Medium (AM1071/AM5071)
Nutrient Agar (AM1074/AM5074)
Nutrient Agar pH 6.8 (AM1075/AM5075)
Nutrient Broth (AM1077/AM5077)
Orange Serum Agar (AM50782)
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits429
APPLICATIONS
Food and Beverage Analysis
PRODUCTSPhenol Red Dextrose Broth (AM50802)
Phenol Red Lactose Broth (AM50802)
Phenol Red Sucrose Broth (AM50808)
Plate Count Agar (AM1081/AM5081)
PNY Medium (AM10812/AM50812)
Potato Dextrose Agar (AM1082/AM5082)
Potato Dextrose Broth (AM1083/AM5083)
Potato Dextrose Agar USP (AM108213/AM50821)
Raka-Ray Agar Base
(Lactic Acid Bacteria Selective Agar Base)(AM10844)
Raka-Ray No. 3 Broth Base
(Lactic Acid Bacteria Selective Broth Base)(AM10845)
Reddy's Differential Agar, Modified (AM50846)
Reinforced Clostridial Agar (AM1085/AM5085)
Rose Bengal Chloramphenicol Agar (AM10857/AM50857)
Sabouraud Dextrose Agar (AM1087/AM5087)
Sabouraud Dextrose Broth (AM1088/AM5088)
Salt Meat Broth (AM50886)
Selenite F Broth (AM1089/AM5089)
Simmons Citrate Agar (AM1090/AM5090)
Skim Milk Agar (AM10901/AM50901)
Soyabean Casein Digest Agar (AM1091/AM5091)
Soyabean Casein Digest Medium (AM1092/AM5092)
SS Agar (AM1093/AM5093)
l
l
l
l ll l l
l
ll l l
l
l
l
l
l
l
l
l
l
l
l
lll
l
l l l l l l
l l lll l
ll
Bac
illus
cer
eus
Aci
duric
and
The
rmop
hilic
Fl
at S
our S
pore
form
ers
Cam
pylo
bact
erC
lost
ridiu
m
Col
iform
s
List
eria
Mes
ophi
lic S
pore
form
ers
(Aer
obic
)
Osm
ophi
lic o
rgan
ism
s
Psy
chro
troph
ic O
rgan
ism
sS
alm
onel
la
Shi
gella
Sta
phyl
ococ
cus
Stre
ptoc
occu
s/E
nter
ococ
cus
Tota
l Pla
te C
ount
Vib
rio
Yeas
ts a
nd M
ould
sYe
rsin
ia e
nter
ocol
itica
Mes
ophi
lic S
pore
form
ers
(Ana
erob
ic)
Iron
Sul
phite
spo
refo
rmer
s
Pro
teol
ytic
mic
roor
gani
sms
Aci
d P
rodu
cing
Bic
robi
olog
y
Aer
ogen
e
Lact
obac
illus
Ent
eroc
occu
s fa
ecal
is
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits 430
APPLICATIONS
Food and Beverage Analysis
PRODUCTSStandard Nutrient Agar /Broth (AM509311/12)
TCBS Agar (AM1095/AM5095)
Tetrathionate Brilliant Green Bile Broth (AM50954)
Tetrathionate Broth Base, Hajna (AM1096/AM5096)
Triple Sugar Iron (TSI) Agar (AM1099/AM5099)
Tryptone Glucose Extract Agar (AM1101/AM5101)
Tryptone Glucose Extract Broth (AM1102/AM5102
Tryptone Phosphate Broth (AM1103/AM5103)
Tryptone Water (AM1104/AM5104)
SS Agar (AM1093/AM5093)
TCBS Agar (AM1095)
Universal Beer agar (AM11042)
Urea Broth Base (AM1106/AM5106)
Urea 40%* (AS028)
Violet Red Bile Agar (AM1107/AM5107)
Violet Red Bile Agar with MUG (AM510712)
Violet Red Bile Broth (AM51072)
Violet Red Bile Glucose Agar (Harmonized)(AMH51073)
Vogel Johnson Agar Base (AM1108/AM5108)
Potassium Tellurite 1%* (AS022)
XLD Agar (AM1112/AM5112)
Yeast Extract Agar (AM1113/AM5113)
Yeast Malt Agar (AM1114/AM5114)
Yersinia Selective Agar Base (AM1116/TM116005)
l l
l
l
l
l l l ll l
l l
l
l
l l
ll
l
l
l l ll
l
l
l l l
l l l
l
ll
l
l
l
Bac
illus
cer
eus
Aci
duric
and
The
rmop
hilic
Fl
at S
our S
pore
form
ers
Cam
pylo
bact
erC
lost
ridiu
m
Col
iform
s
List
eria
Mes
ophi
lic S
pore
form
ers
(Aer
obic
)
Osm
ophi
lic o
rgan
ism
s
Psy
chro
troph
ic O
rgan
ism
sS
alm
onel
la
Shi
gella
Sta
phyl
ococ
cus
Stre
ptoc
occu
s/E
nter
ococ
cus
Tota
l Pla
te C
ount
Vib
rio
Yeas
ts a
nd M
ould
sYe
rsin
ia e
nter
ocol
itica
Mes
ophi
lic S
pore
form
ers
(Ana
erob
ic)
Iron
Sul
phite
spo
refo
rmer
s
Pro
teol
ytic
mic
roor
gani
sms
Aci
d P
rodu
cing
Bic
robi
olog
y
Aer
ogen
e
Lact
obac
illus
Ent
eroc
occu
s fa
ecal
is
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits431
APPLICATIONS
Food and Beverage Analysis
PRODUCTS
Bac
illus
cer
eus
Aci
duric
and
The
rmop
hilic
Fl
at S
our S
pore
form
ers
Cam
pylo
bact
erC
lost
ridiu
m
Col
iform
s
List
eria
Mes
ophi
lic S
pore
form
ers
(Aer
obic
)
Osm
ophi
lic o
rgan
ism
s
Psy
chro
troph
ic O
rgan
ism
sS
alm
onel
la
Shi
gella
Sta
phyl
ococ
cus
Stre
ptoc
occu
s/E
nter
ococ
cus
Tota
l Pla
te C
ount
Vib
rio
Yeas
ts a
nd M
ould
sYe
rsin
ia e
nter
ocol
itica
Mes
ophi
lic S
pore
form
ers
(Ana
erob
ic)
Iron
Sul
phite
spo
refo
rmer
s
Pro
teol
ytic
mic
roor
gani
sms
Aci
d P
rodu
cing
Bic
robi
olog
y
Aer
ogen
e
Lact
obac
illus
Ent
eroc
occu
s fa
ecal
is
Yersinia Selective Supplement *(AS029)
W.L. Differential Broth (AM51091)
W L Nutrient Agar (AM51092)
l
l
lW L Nutrient Broth (AM1110/AM5111)
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits 432
Dairy Analysis
Bac
illus
Spe
cies
(and
oth
er s
pore
be
arer
s)
APPLICATIONS
PRODUCTS
Col
iform
s
Ana
erob
ic
Mic
roor
gani
sms
(Incl
udin
g C
lost
ridia
)
Lact
obac
illi
List
eria
m
onoc
ytog
enes
Sal
mon
ella
/Shi
gella
Sta
ndar
d P
late
C
ount
Sta
phyl
ococ
cus
Stre
ptoc
occu
s/E
nter
ococ
cus
Yeas
t & M
ould
Yers
inia
ent
eroc
oliti
ca
Cam
pylo
bact
er
Pro
teol
ytic
act
ivity
AK Agar No. 2 (AM5909)
Azide Blood Agar Base (AM1006/AM5006)
Azide Dextrose Broth (AM50061)
Bacillus Cereus Agar Base (AM1009/5009)
Polymyxin B Selective Supplement* (AS021)
Egg Yolk Supplement* (AS010)
Baird Parker Agar Base (AM1011/AM5011)
B P Sulpha Supplement* (AS005)
Egg Yolk Emulsion* (A010)
Egg Yolk Tellurite Emulsion* (AS011)
Potassium Tellurite 3.5%* (AS023)
Bile Esculin Agar (AM1012/AM5012)
Bismuth Sulphite Agar (AM1013/AM5013)
Brain Heart Infusion Agar (AM1016/AM5016)
Brain Heart Infusion Broth (AM1017/AM5017)
Brilliant Green Agar Modified (AM1018/AM5018)
Sulpha Supplement* (AS027)
Brilliant Green Bile Broth 2% (AM1020/AM5020)
Campylobacter Agar Base (AM50218)
Chloramphenicol Yeast Glucose Agar (AM1024/AM5024)
Cooked Meat Medium (AM1030/AM5030)
Czpek Dox Agar (AM10302/AM50302)
Decarboxylase Agar Base (AM503023)
Deoxycholate agar (AM10303/AM50303)
l
l
l
l
l
l
l
l
l l
l
l
l
l
l
l
l
l
l
Buffered Peptone Water (AM10211/50211)
Ent
erob
acte
r and
K
lebs
iella
spe
cies
Pse
udom
onas
ae
rugi
nosa
,
Ser
ratia
mar
cesc
ens
Clo
strid
ia
Pro
teus
mira
bilis
Ent
eroc
occu
s fa
ecal
is
Aer
ugin
osa
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits433
Bac
illus
Spe
cies
(and
oth
er s
pore
be
arer
s)
Col
iform
s
Ana
erob
ic
Mic
roor
gani
sms
(Incl
udin
g C
lost
ridia
)
Lact
obac
illi
List
eria
m
onoc
ytog
enes
Sal
mon
ella
/Shi
gella
Sta
ndar
d P
late
C
ount
Sta
phyl
ococ
cus
Stre
ptoc
occu
s/E
nter
ococ
cus
Yeas
t & M
ould
Yers
inia
ent
eroc
oliti
ca
Cam
pylo
bact
er
Pro
teol
ytic
act
ivity
Ent
erob
acte
r and
K
lebs
iella
spe
cies
Pse
udom
onas
ae
rugi
nosa
,
Ser
ratia
mar
cesc
ens
Clo
strid
ia
Pro
teus
mira
bilis
Ent
eroc
occu
s fa
ecal
is
Aer
ugin
osa
Dairy Analysis
PRODUCTSDifferential Reinforced Clostridial Broth (AM1038/AM5038)
EC Broth (AM1039/AM5039)
EMB Agar, Levine (AM1040/AM5040)
Endo Agar (AM1040/AM5040)
Fluid Lactose Medium (AM1042/AM5042)
Fluid Selenite Cystine Medium (AM1044/AM5044)
Hektoen Enteric Agar (AM104821/AM504823)
Iron Sulphite Agar (AM10483/AM50483)
Lactobacillus MRS Agar (AM1051/AM5051)
Lactose Broth (AM1052/AM5052)
LPM Agar Base (AM10575)
Lysine Iron Agar (AM10576/AM50576)
MacConkey Broth Harmonized (AMH5063)
MacConkey Broth USP (AM50636)
MacConkey Broth Purple with BCP ISO/BIS/BP/IPBCP
l
l
l
l
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l
l
l
l
l
l
l
l
l
l
l
l
l
l
l
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l
Lactobacillus MRS Broth (AM10512)
Lauryl Tryptose Broth (AM1053/AM5053)
Lee's Agar (AM50532)
Listeria Identification Agar Base, PALCAM, (AM1055/AM5055)
Listeria Oxford Medium Base (AM105512/AM505512)
Oxford Listeria Supplement* (AS0201)
Listeria Moxlactam Supplement* (AS0171)
Listeria Selective Supplement* (AS018)
MacConkey Agar
MacConkey Agar with Crystal Violet, NaCl and 0.15%Bile Salts (AM1059/AM5059)
MacConkey Broth with Bromocresol Purple (AM1063/AM5063)
APPLICATIONS
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits 434
Bac
illus
Spe
cies
(and
oth
er s
pore
be
arer
s)
Col
iform
s
Ana
erob
ic
Mic
roor
gani
sms
(Incl
udin
g C
lost
ridia
)
Lact
obac
illi
List
eria
m
onoc
ytog
enes
Sal
mon
ella
/Shi
gella
Sta
ndar
d P
late
C
ount
Sta
phyl
ococ
cus
Stre
ptoc
occu
s/E
nter
ococ
cus
Yeas
t & M
ould
Yers
inia
ent
eroc
oliti
ca
Cam
pylo
bact
er
Pro
teol
ytic
act
ivity
Ent
erob
acte
r and
K
lebs
iella
spe
cies
Pse
udom
onas
ae
rugi
nosa
,
Ser
ratia
mar
cesc
ens
Clo
strid
ia
Pro
teus
mira
bilis
Ent
eroc
occu
s fa
ecal
is
Aer
ugin
osa
Dairy Analysis
PRODUCTSMalonate Broth, Ewing Modified (AM1065/AM5065)
Malt Agar (AM1066/AM5066)
Malt Extract Agar (AM1067/AM5067)
Mannitol Salt Agar (AM1069/AM5069)
Milk Agar (AM10692711/AM50692711)
Modified Rappaport Vassiliadis Medium for Water Testing BIS/ISO (AM506929/AM506930)
Motility Test Medium (AM506933)
MR-VP Medium (AM1071/AM5071)
Nitrate Broth
Nutrient Agar (AM1074/AM5074)
Nutrient Broth (AM1077/AM5077)
Plate Count Agar (AM1081/AM5081)
Phenylalanine Agar (AM108091/AM508091)
Potato Dextrose Agar (AM1082/AM5082)
Potato Dextrose Broth (AM1083/AM5083)
Preston Selective Supplement (Campylobacter Selective Supplement IV, Modified) (AS231)
Reddy's Differential Agar Modified (AM50846)
Reinforced Clostridial Agar (AM1085/AM5085)
Reinforced Clostridial Broth
Rogosa SL Agar (AM50854)
Rogosa SL Broth (AM50855)
Rose Bengal Agar Base (AM50856)
Rose Bengal Chloramphenicol Agar (AM10857/AM50857)
SIM Medium (AM50892)
Simmons Citrate Agar (AM1090/5090)
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APPLICATIONS
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits435
Bac
illus
Spe
cies
(and
oth
er s
pore
be
arer
s)
Col
iform
s
Ana
erob
ic
Mic
roor
gani
sms
(Incl
udin
g C
lost
ridia
)
Lact
obac
illi
List
eria
m
onoc
ytog
enes
Sal
mon
ella
/Shi
gella
Sta
ndar
d P
late
C
ount
Sta
phyl
ococ
cus
Stre
ptoc
occu
s/E
nter
ococ
cus
Yeas
t & M
ould
Yers
inia
ent
eroc
oliti
ca
Cam
pylo
bact
er
Pro
teol
ytic
act
ivity
Ent
erob
acte
r and
K
lebs
iella
spe
cies
Pse
udom
onas
ae
rugi
nosa
,
Ser
ratia
mar
cesc
ens
Clo
strid
ia
Pro
teus
mira
bilis
Ent
eroc
occu
s fa
ecal
is
Aer
ugin
osa
Dairy Analysis
Skim Milk Agar (AM10901/AM50901)
Soyabean Casein Digest Agar (AM1091/AM5091)
Soyabean Casein Digest Medium (AM1092/AM5092)
Soyabean Casein Digest Agar with Lecithin and Tween 80*
SS Agar (AM1093/AM5093)
Staphylococcus Agar No.110 (AM10932/AM50932)
Tetrathionate Broth Base, Hajna (AM1096/AM5096)
Tetrathionate Broth Base
Tomato Juice Agar (AM1097/AM5097)
Tomato Juice Agar, Special (AM1098/AM5098)
Triple Sugar Iron (TSI) Agar (AM1099/AM5099)
Tryptone Glucose Beef Extract Agar (TGB Agar)(AM1102/AM5102)
Tryptone Glucose Extract Agar (AM1101/AM5101)
Tryptone Phosphate Broth (AM1103/AM5103)
Tryptone Soya Agar with Lecithin and Tween 80* (AM11031/AM51031)
Tryptone Water (AM1104/AM5104)
Urea Agar Base, Christensen (AM1105/AM5105)
Urea Broth Base (AM1106/AM5106)
Urea 40%* (AS028)
Violet Red Bile Agar Base (AM1107/AM5107)
Violet Red Bile Agar with MUG (AM510712)
Violet Red Bile Glucose Agar (Harmonized) (AMH51073)
Vogel Johnson Agar Base (AM1108/AM5108)
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(AM50927)
Urea 40%* (AS028)
PRODUCTS
APPLICATIONS
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits 436
Bac
illus
Spe
cies
(and
oth
er s
pore
be
arer
s)
Col
iform
s
Ana
erob
ic
Mic
roor
gani
sms
(Incl
udin
g C
lost
ridia
)
Lact
obac
illi
List
eria
m
onoc
ytog
enes
Sal
mon
ella
/Shi
gella
Sta
ndar
d P
late
C
ount
Sta
phyl
ococ
cus
Stre
ptoc
occu
s/E
nter
ococ
cus
Yeas
t & M
ould
Yers
inia
ent
eroc
oliti
ca
Cam
pylo
bact
er
Pro
teol
ytic
act
ivity
Ent
erob
acte
r and
K
lebs
iella
spe
cies
Pse
udom
onas
ae
rugi
nosa
,
Ser
ratia
mar
cesc
ens
Clo
strid
ia
Pro
teus
mira
bilis
Ent
eroc
occu
s fa
ecal
is
Aer
ugin
osa
Dairy Analysis
PRODUCTS
APPLICATIONS
Potassium Tellurite 1%* (AS022)
XLD Agar (AM1112/AM5112)
Yeast Extract Chloramphenicol Dextrose Agar (AM51131)
Yersinia Selective Agar Base (AM1116/TM116005)
Yersinia Selective Supplement* (AS029)
l
l
l
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits437
Ant
ibio
tic A
ssay
M
edia
E. c
oli
Pse
udom
onas
Sal
mon
ella
e
Sta
phyl
ococ
cus
Tota
l Aer
obic
M
icro
bial
Cou
nt
Yeas
t and
Mou
ld
Cou
nt
Ste
rility
Tes
ting
Vita
min
Ass
ay
Lact
obac
illus
Cou
nt
Microbial Limit Test
Pharmaceutical Analysis
PRODUCTSAlternative Thioglycollate Medium (AM1900/AM5900)
Antibiotic Assay Medium A (No. 1, Seed Agar) (AM1002/AM5002)
Antibiotic Assay Medium C (No. 3, Assay Broth) (AM1003/AM5003)
Antiobiotic Assay Medium E (No. 5) (Streptomycin Assay agar with yeast Extarct) (AM50031)
Antiobiotic Assay Medium F (No. 8) (Base Agar with low pH)
Antibiotic Assay Medium No 11 (Neomycin, Erythromycin Assay Agar) (AM1004/AM5004)
Antibiotic Assay Medium G (N. 19) (AM500411)
Antiobiotic Assay Medium No. 32 (AM500412)
Antifungal Assay Agar (AM10041/AM50041)
B12 Assay Agar using E.coli Mutant Culture (AM1007/AM5007 )
B12 Assay Medium (AM10071)
B12 Maintenance Medium for E.coli Mutant (AM1008/AM5008)
Baird Parker Agar Base (AM1011/AM5011)
B P Sulpha Supplement* (AS005)
Egg Yolk Emulsion* (AS010)
Egg Yolk Tellurite Emulsion* (AS011)
Potassium Tellurite 3.5%* (AS023)
Bismuth Sulphite Agar (AM1013/AM5013)
Brilliant Green Agar Modified (AM1018/AM5018)
Sulpha Supplement* (AS027)
Cetrimide Agar Base (AM1022/AM5022)
Columbia agar Base
Nalidixic Selective Supplement* (AS020)
Deoxycholate Citrate Agar (AM1031/AM5031)
E.E. Broth, Mossel (AM103913/AM503913)
EMB Agar, Levine (AM1040/AM5040)
l
l
l
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l
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l
l
l
l
l
l
l
l
(AM50032)
APPLICATIONS
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits 438
Pharmaceutical Analysis
APPLICATIONS
PRODUCTS
Ant
ibio
tic A
ssay
M
edia
E. c
oli
Pse
udom
onas
Sal
mon
ella
e
Sta
phyl
ococ
cus
Tota
l Aer
obic
M
icro
bial
Cou
nt
Yeas
t and
Mou
ld
Cou
nt
Ste
rility
Tes
ting
Vita
min
Ass
ay
Lact
obac
illus
Cou
nt
Microbial Limit Test
Fluid Lactose Medium (AM1042/AM5042)
Fluid Selenite Cystine Medium (AM1044/AM5044)
Fluid Thioglycollate Medium (AM1045/AM5045)
Glucose Yeast Extract Agar (AM1048/AM5048)
Inactivator Broth (AM104824/AM504824)
Lactose Broth (AM1052/AM5052)
Lactose Sulphite Broth Base (AM50525)
Lysine Iron Agar (AM10576/AM50576)
MacConkey Agar with Crystal Violet,NaCl and 0.15% Bile Salts (AM1059/AM5059)
MacConkey Broth
(AM10785)
(AM10787)
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l
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l
l
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l
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l
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l
l ll l
MacConkey Broth with Bromocresol Purple (AM1063/AM5063)
Mannitol Salt Agar (AM1069/AM5069)
Nutrient Broth (AM1077/AM5077)
Pantothenate Assay Medium (AM10784)
Pantothenate Assay Medium, AOAC
Pantothenate Inoculum Broth
Pseudomonas Agar (For Fluorescein) (AM108411/AM508411)
Pseudomonas Agar (For Pyocyanin) (AM108414)
Peptone Water (AM1079/AM5079)
Potato Dextrose Agar (AM1082/AM5082)
Sabouraud Chloramphenicol Agar (AM1086/AM5086)
Sabouraud Dextrose Agar (AM1087/AM5087)
Sabouraud Dextrose Broth (AM1088/AM5088)
Selenite F Broth (AM1089/AM5089)
Soyabean Casein Digest Agar (AM1091/AM5091)
Soyabean Casein Digest Medium (AM1092/AM5092)
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits439
Pharmaceutical Analysis
APPLICATIONS
PRODUCTS
Ant
ibio
tic A
ssay
M
edia
E. c
oli
Pse
udom
onas
Sal
mon
ella
e
Sta
phyl
ococ
cus
Tota
l Aer
obic
M
icro
bial
Cou
nt
Yeas
t and
Mou
ld
Cou
nt
Ste
rility
Tes
ting
Vita
min
Ass
ay
Lact
obac
illus
Cou
nt
Microbial Limit Test
Soyabean Casein Digest Medium Sterile Powder (AM50925/AM50925-5K)
Tetrathionate Brilliant Green Bile Broth (AM50954)
Tetrathionate Broth Base, Hajna (AM1096/AM5096)
Triple Sugar Iron (TSI) Agar (AM1099/AM5099)
Urea Broth Base (AM1105)
Urea 40%* (AS028)
Vogel Johnson Agar Base (AM1108/AM5108)
Potassium Tellurite 1%*(AS022)
W.L. Differential Broth (AM51091)
XLD Agar (AM1112/AM5112)
l l l
l
l
l
l
l
l
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits 440
APPLICATIONS
Water and Wastewater Analysis
PRODUCTS Tota
l Pla
te C
ount
Sta
phyl
ococ
cus
MP
N C
onfir
med
MP
N P
resu
mpt
ive
Iden
tific
atio
n &
B
ioch
emic
al T
ests
Sel
ectiv
e Is
olat
ion
Pre
-Enr
ichm
ent/
Sel
ectiv
e E
nric
hmen
t
Pse
udom
onas
Legi
onel
la S
peci
es
Kle
bsie
lla
Feca
l Stre
ptoc
occi
Col
ipha
ge D
etec
tion
Diff
eren
tiatio
n of
Fec
al &
Non
feca
l Col
iform
Feca
l Col
iform
s
MP
N C
ompl
eted
Tes
t
MP
N C
onfir
med
Tes
t
MP
N P
resu
mpt
ive
Test
Clo
strid
ia
Vib
rio
Yeas
ts a
nd M
ould
sYe
rsin
ia E
nter
ocol
itica
Total coliformSalmonella/Shigella
Qualitative Tests Quantitative Tests
Alkaline Peptone water (AM1910/AM5910)
Aspargine Proline Broth (AM1005/AM5005)
Azide Blood Agar Base (AM1006/AM5006)
Azide Dextrose Broth (AM50061)
Baird Parker Agar Base (AM1011/AM5011)
BP Sulpha Supplement* (AS005)
Egg Yolk Emulsion* (A010)
EY Tellurite Enrichment* (AS011)
Potassium Tellurite 3.5%* (AS023)
Beef Extract Agar (AM10111/AM50111)
Bile Esculin Agar Base (AM1012/AM5012)
Bile Esculine Azide Agar (AM501212)
Bismuth Sulphite Agar (AM1013/AM5013)
Brain Heart Infusion Agar (AM1016/AM5016)
Brain Heart Infusion Broth (AM1017/AM5017)
Brilliant Green Agar Modified (AM1018/AM5018)
Sulpha Supplement* (AS027)
Brilliant Green Bile Broth 2% (AM1020/AM5020)
Cetrimide Agar Base (AM1022/AM5022)
Nalidixic Selective Supplement* (AS020)
Cetrimide Broth (AM1023/AM5023)
Cetrimide Broth (Am1023/AM5023)
Corn Meal Agar (AM10301/AM50301)
Deoxycholate Citrate Agar (AM1031/AM5031)
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l
l l
l
l
l
l
l
l
l
l l
ll
l
l
l
l
Cam
pylo
bact
er
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits441
APPLICATIONS
Water and Wastewater Analysis
Tota
l Pla
te C
ount
Sta
phyl
ococ
cus
MP
N C
onfir
med
MP
N P
resu
mpt
ive
Iden
tific
atio
n &
B
ioch
emic
al T
ests
Sel
ectiv
e Is
olat
ion
Pre
-Enr
ichm
ent/
Sel
ectiv
e E
nric
hmen
t
Pse
udom
onas
Legi
onel
la S
peci
es
Kle
bsie
lla
Feca
l Stre
ptoc
occi
Col
ipha
ge D
etec
tion
Diff
eren
tiatio
n of
Fec
al &
Non
feca
l Col
iform
Feca
l Col
iform
s
MP
N C
ompl
eted
Tes
t
MP
N C
onfir
med
Tes
t
MP
N P
resu
mpt
ive
Test
Clo
strid
ia
Vib
rio
Yeas
ts a
nd M
ould
sYe
rsin
ia E
nter
ocol
itica
Total coliformSalmonella/Shigella
Qualitative Tests Quantitative Tests
Differential Reinforced Clostridial Broth (AM1038/AM5038)
EC Broth (AM1039/AM5039)
ECD Agar (AM503912)
EMB Agar, Levine (AM1040/AM5040)
Endo Agar (AM1041/AM5041)
(AM5063)
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l
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l l
Fluid Lactose Medium (AM1042/AM5042)
Fluid Selenite Cystine Medium (AM1044/AM5044)
Kligler Iron Agar (AM1050/AM5050)
Lactose Broth (AM1052/AM5052)
Lauryl Tryptose Broth (AM1053/AM5053)
Legionella Agar Base (AM1054/AM5054)
Legionella Growth Supplement* (AS016)
Legionella Selective Supplement* (AS017)
Lysine Iron Agar (AM10576/AM50576)
MacConkey Agar with Crystal Violet, NaCl and with 0.15% Bile Salts (AM1059/AM5059)
MacConkey Agar without Crystal Violet and with 0.15% Bile Salts(AM1060/AM5060)
MacConkey Agar without Crystal Violet, NaCl and with 0.5% Sodium Taurocholate (AM1061/AM5061)
MacConkey Broth Double Strength with Neutral Red BIS(AM10621/AM50621)
MacConkey Broth Harmonized
MacConkey Broth USP (AM50636)
MacConkey Broth with Neutral Red (AM1064/AM5064)
MacConkey Broth Purple W/Bromocresol Purple (AM1063/AM5063)
PRODUCTS
Cam
pylo
bact
er
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits 442
APPLICATIONS
Water and Wastewater Analysis
PRODUCTS Tota
l Pla
te C
ount
Sta
phyl
ococ
cus
MP
N C
onfir
med
MP
N P
resu
mpt
ive
Iden
tific
atio
n &
B
ioch
emic
al T
ests
Sel
ectiv
e Is
olat
ion
Pre
-Enr
ichm
ent/
Sel
ectiv
e E
nric
hmen
t
Pse
udom
onas
Legi
onel
la S
peci
es
Kle
bsie
lla
Feca
l Stre
ptoc
occi
Col
ipha
ge D
etec
tion
Diff
eren
tiatio
n of
Fec
al &
Non
feca
l Col
iform
Feca
l Col
iform
s
MP
N C
ompl
eted
Tes
t
MP
N C
onfir
med
Tes
t
MP
N P
resu
mpt
ive
Test
Clo
strid
ia
Vib
rio
Yeas
ts a
nd M
ould
sYe
rsin
ia E
nter
ocol
itica
Total coliformSalmonella/Shigella
Qualitative Tests Quantitative Tests
Cam
pylo
bact
er
MacConkey Broth Purple with BCP ISO/BIS/BP/IPBCP
Malonate Broth Ewing, Modified (AM1065/AM5065)
Malt Agar (AM1066/5066)
Malt Extract Agar (AM1067/5067)
Mannitol Motility Test Medium (AM10681/AM50681)
Mannitol Salt Agar (AM1069/AM5069)
M-Endo Agar LES (AM106921/AM506921)
M-Endo Broth (AM106922/AM506922)
M-FC Agar Base (AM506923)
M-FC Broth Base (AM506924)
M-(HPC) Heterotrophic Plate Count Agar Base (AM506925)
M-(HPC) Heterotrophic Plate Count Broth Base (AM506926)
MR-VP Medium (AM1071/AM5071)
Milk Agar AM10692711/AM50692711)
MUG Lauryl Sulphate Broth (AM50721)
Nutrient Agar pH 6.8 (AM1075/AM5075)
Nutrient Broth (AM1077/AM5077)
PA Broth (AM507831)
Phenol Red Dextrose Broth (AM50802)
Phenol Red Lactose Broth (AM50803)
Phenol Red Mannitol Broth (AM50806)
Phenol Red Sucrose Broth (AM50808)
Phenol Red Xylose Broth (AM50809)
Phenylalanine Agar (AM108091/AM508091)
l
l
l
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l
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l
l
l
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits443
APPLICATIONS
Water and Wastewater Analysis
PRODUCTS Tota
l Pla
te C
ount
Sta
phyl
ococ
cus
MP
N C
onfir
med
MP
N P
resu
mpt
ive
Iden
tific
atio
n &
B
ioch
emic
al T
ests
Sel
ectiv
e Is
olat
ion
Pre
-Enr
ichm
ent/
Sel
ectiv
e E
nric
hmen
t
Pse
udom
onas
Legi
onel
la S
peci
es
Kle
bsie
lla
Feca
l Stre
ptoc
occi
Col
ipha
ge D
etec
tion
Diff
eren
tiatio
n of
Fec
al &
Non
feca
l Col
iform
Feca
l Col
iform
s
MP
N C
ompl
eted
Tes
t
MP
N C
onfir
med
Tes
t
MP
N P
resu
mpt
ive
Test
Clo
strid
ia
Vib
rio
Yeas
ts a
nd M
ould
sYe
rsin
ia E
nter
ocol
itica
Total coliformSalmonella/Shigella
Qualitative Tests Quantitative Tests
Cam
pylo
bact
er
Plate Count Agar (AM1081/AM5081)
Preston Agar Base (AM10831/AM50831)
Preston Selective Supplement (Campylobacter Selective Supplement IV, Modified) (AS0231)
Pseudomonas Agar (For Fluorescein) (AM108414/AM508415)
Pseudomonas Agar (For Pyocyanin) (AM108411/AM508411)
Pseudomonas Agar Base (AM1084/AM5084)
Cetrinix Supplement* (AS008)
CFC Supplement* (AS009)
Pseudomonas Asparagine Broth (AM108418/AM508418)
R-2A Agar (AM10841/AM50841)
R-2A Agar (Agar Medium S)
Rappaport Vassiliadis Salmonella Enrichment Broth
(Harmonized) (AMH508453)
Reinforced Clostridial Agar (AM1085/AM5085)
Selenite F Broth (AM1089/AM5089)
SIM Medium (AM50892)
Simmions Citrate Agar (AM1090/AM5090)
Soyabean Casein Digest Agar (AM1091/AM5091)
Soyabean Casein Digest Medium (AM1092/AM5092)
SS Agar (AM1093/AM5093)
TCBS Agar (AM1095/AM5095)
Tergitol-7 Agar Base (AM10951/AM50951)
Tergitol-7 Broth (AM50953)
l
l
l
l
l
l
l
l l
l l l
l
l l
l
l
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits 444
APPLICATIONS
Water and Wastewater Analysis
PRODUCTS Tota
l Pla
te C
ount
Sta
phyl
ococ
cus
MP
N C
onfir
med
MP
N P
resu
mpt
ive
Iden
tific
atio
n &
B
ioch
emic
al T
ests
Sel
ectiv
e Is
olat
ion
Pre
-Enr
ichm
ent/
Sel
ectiv
e E
nric
hmen
t
Pse
udom
onas
Legi
onel
la S
peci
es
Kle
bsie
lla
Feca
l Stre
ptoc
occi
Col
ipha
ge D
etec
tion
Diff
eren
tiatio
n of
Fec
al &
Non
feca
l Col
iform
Feca
l Col
iform
s
MP
N C
ompl
eted
Tes
t
MP
N C
onfir
med
Tes
t
MP
N P
resu
mpt
ive
Test
Clo
strid
ia
Vib
rio
Yeas
ts a
nd M
ould
sYe
rsin
ia E
nter
ocol
itica
Total coliformSalmonella/Shigella
Qualitative Tests Quantitative Tests
Cam
pylo
bact
er
Tetrathionate Broth Base, Hajna (AM1096/AM5096)
Triple Sugar Iron Agar (AM1099/AM5099)
Tryptone Glucose Beef Extract Agar (TGB Agar) (AM11002/AM51002)
Tryptone Glucose Extract Agar (AM1101/AM5101)
Tryptone Water (AM1104/AM5104)
Urea Agar Base, Christensen (AM1105/AM5105)
Urea 40%* (AS028)
Urea Broth Base (AM1106/AM5106)
Urea 40%* (AS028)
Violet Red Bile Agar (AM1107/AM5107)
Violet Red Bile Broth (AM51072)
XLD Agar (AM1112/AM5112)
Yeast Malt Agar (AM1114/AM5114)
Yeast Malt Broth (AM1115/AM5115)
Yersinia Selective Agar Base (AM1116/TM5116)
Yersinia Selective Supplement* (AS029)
l l
l l l l
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l l
l l
l
l
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l l
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l
l
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits445
APPLICATIONS
Veterinary Testing
Yeas
t & M
ould
s
Vib
rio
Stre
ptoc
occi
Sta
phyl
ococ
ci
Sal
mon
ella
/Shi
gella
Pse
udom
onas
Myc
obac
teriu
m
List
eria
Gen
eral
Pur
pose
Cul
ture
Med
ia
Col
iform
s
Bru
cella
Ana
erob
es (I
nclu
ding
Clo
strid
ia)
PRODUCTSAzide Blood Agar Base (AM1006/AM5006)
Baird Parker Agar Base (AM1011/AM5011)
BP Sulpha Supplement* (AS005)
Egg Yolk Emulsion*(A010)
Egg Yolk Tellurite Emulsion* (AS011)
Potassium Tellurite 3.5%* (AS023)
Bismuth Sulphite Agar (AM1013/AM5013)
Brain Heart Infusion Agar (AM1016/AM5016)
Brain Heart Infusion Broth (AM1017/AM5017)
Brilliant Green Agar Modified (AM1018/AM5018)
Sulpha Supplement* (AS027)
Cetrimide Agar Base (AM1022/AM5022)
Nalidixic Selective Supplement* (AS022)
Columbia Blood Agar Base (AM1029/AM5029)
Brucella Selective Supplement* (AS006)
Staph-Strepto Supplement* (AS025)
Strepto Supplement* (AS026)
Cooked Meat Medium (AM1030/AM5030)
Corn Meal Agar (AM10301/AM50301)
DNase Test Agar Base (AM10381)
EMB Agar, Levine (AM1040/5040)
Endo Agar (AM1041/AM5041)
ll
l
l
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l
l
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l
l l
l
l
l
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits 446
APPLICATIONS
Veterinary Testing
Yeas
t & M
ould
s
Vib
rio
Stre
ptoc
occi
Sta
phyl
ococ
ci
Sal
mon
ella
/Shi
gella
Pse
udom
onas
Myc
obac
teriu
m
List
eria
Gen
eral
Pur
pose
Cul
ture
Med
ia
Col
iform
s
Bru
cella
Ana
erob
es (I
nclu
ding
Clo
strid
ia)
PRODUCTSListeria Identification Agar Base PALCAM (AM1055/AM5055)
Fluid Selenite Cystine Medium (AM1044/AM5044)
Fluid Thioglycollate Medium (AM1045/AM5045)
Heart Infusion Agar (AM10481/AM50481)
Listeria Selective Supplement* (AS018)
Liver Infusion Agar (AM10551/AM50551)
Liver Infusion Broth (AM10552/AM50552)
Lowenstein Jensen Medium Base (AM1057/AM5057)
MacConkey Agar Base (AM1058/AM5058)
Malt Agar (AM1066/AM5066)
Malt Extract Agar (AM1067/AM5067)
Mannitol Salt Agar (AM1069/AM5069)
MR-VP Medium (AM1071/AM5071)
Potato Dextrose Agar (AM1082/AM5082)
Pseudomonas Agar Base (AM1084/AM5084)
Cetrinix Supplement* (AS008)
CFC Supplement* (AS009)
Reinforced Clostridial Agar (AM1085/AM5085)
Sabouraud Dextrose Agar (AM1087/AM5087)
Sabouraud Dextrose Broth (AM1088/AM5088)
Selenite F Broth (AM1089/AM5089)
Simmons Citrate Agar (AM1090/AM5090)
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l
l l
l
l
l
l
l
l
l
l
l l l
l
l
l
l
l
l
l l
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits447
APPLICATIONS
Veterinary Testing
Yeas
t & M
ould
s
Vib
rio
Stre
ptoc
occi
Sta
phyl
ococ
ci
Sal
mon
ella
/Shi
gella
Pse
udom
onas
Myc
obac
teriu
m
List
eria
Gen
eral
Pur
pose
Cul
ture
Med
ia
Col
iform
s
Bru
cella
Ana
erob
es (I
nclu
ding
Clo
strid
ia)
PRODUCTSSoyabean Casein Digest Agar (AM1091/AM5091)
Soyabean Casein Digest Medium (AM1092/AM5092)
SS Agar (AM1093/AM5093)
TCBS Agar (AM1095/AM5095)
Tetrathionate Broth Base, Hajna (AM1096/AM5096)
Triple Sugar Iron (TSI) Agar (AM1099/AM5099)
Tryptose Agar (AM11041/AM51041)
Urea Agar Base, Christensen (AM1105/AM5105)
Urea 40%* (AS028)
Violet Red Bile Agar (AM1107/AM5107)
Vogel Johnson Agar Base (AM1108/AM5108)
Potassium Tellurite 1%* (AS022)
XLD Agar (AM1112/AM5112)
Yeast Malt Agar (AM1114/AM5114)
Yeast Malt Broth (AM1116/AM5116)
l
l
l
l
l
l l
l
l l
l
l
l
l
l
0* - Store between 2-8 C.
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits 448
Products for Agriculture
Ashby's Glucose Agar-- Nitrogen fixing bacteria
Ashby's Mannitol Agar-- Nitrogen fixing bacteria
Czpek Dox Agar
Cetrimide agar base
Cetrimide Broth
Jensen Broth
Jensen Medium
Pseudomonas Agar (For Pyocyanin)
Pseudomonas Agar (For Fluorescein)
Yeast Mannitol agar with 1.5% Agar
Yeast Mannitol Agar with Congo Red
Yeast Mannitol Broth
Pikovskaya's agar
Pikovskaya's Broth
l
l
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l
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0* - Store between 2-8 C.
PRODUCTS
APPLICATIONS
Yeas
t & M
ould
s
Pen
icill
ium
not
atum
Pse
udom
onas
Rhi
zobi
um
Asp
ergi
llus
nige
r
Azo
toba
cter
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits449
Products for Brewery and Fermentation Analysis
APPLICATIONS
Aga
r Med
ia
Liqu
id M
edia
Col
iform
s
Lact
obac
illi
Fermentation Process
Fluid Lactose Medium (AM1042/AM5042)
Lactobacillus MRS Agar (AM1051/AM5051)
MacConkey Agar with Crystal Violet, NaCl and 0.15%Bile Salts (AM1059/AM5059)
MacConkey Broth Purple with Bromocresol Purple (AM1063/AM5063)
Malt Agar (AM1066/AM5066)
WL Differential Agar (AM1109/AM5109)
WL Nutrient Broth (AM1110/AM5110)
Wort Agar (AM1111/AM5111)
Yeast Malt Agar (AM1114/AM5114)
l
l
l
l
l
l
l l
l l
l
Brewery Analysis
PRODUCTS
0* - Store between 2-8 C.
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits 450
Products for Medical and
Research Institutes
APPLICATIONS
PRODUCTS
Yers
inia
ent
eroc
oliti
ca
Yeas
t & M
ould
s
Vib
rio
Stre
ptoc
occu
s
Sta
phyl
ococ
cus
Shi
gella
Sal
mon
ella
Pro
teus
Pse
udom
onas
Nei
sser
ia
Myc
opla
sma
Myc
obac
teria
List
eria
Legi
onel
la
Kle
bsie
lla
Hae
mop
hilis
E. c
oli
Cor
yneb
acte
rium
Clo
strid
ia
Bru
cella
Bor
dete
lla
Bac
tero
ides
0C - Cultivation, E - Enrichment, I - Identification, N - Non-Selective and S - Selective Isolation. * - Store between 2-8 C.
Anaerobic Agar (AM1000/AM5000)
Azide Blood Agar Base (AM1006/AM5006)
Bacteroides Bile Esculin Agar (AM1010/AM5010)
Bacteroides Selective Supplement* (AS001)
Baird Parker Agar Base (AM1011/AM5011)
B P Sulpha Supplement* (AS005)
Egg Yolk Emulsion* (AS010)
Egg Yolk Tellurite Emulsion* (AS011)
Potassium Tellurite 3.5%* (AS023)
Bismuth Sulphite Agar (AM1013/AM5013)
Blood Agar Base (AM1014/AM5014)
Bordet Gengou Agar Base (AM1015/AM5015)
Bordetella Selective Supplement* (AS004)
Brain Heart Infusion Broth (AM1017/AM5017)
Briliiant Green Agar Modified (AM1018/AM5018)
Sulpha Supplement* (AS027)
Brilliant Green Bile Agar (AM1019/AM5019)
Brilliant Green Bile Broth 2% (AM1020/AM5020)
Cetrimide Agar Base (AM1022/AM5022)
Nalidixic Selective Supplement* (AS020)
Cetrimide Broth (AM1023/AM5023)
Chloramphenicol Yeast Glucose Agar (AM1024/AM5024)
CLED Agar with Andrade indicator (AM1026/AM5026)
CLED Agar with Bromothymol Blue (AM1027/AM5027)
Coagulase Mannitol Agar Base (AM1028/AM50258)
C
C C
C/I/S
S
S
C/N C/N
C/S
E
S
N
E
S
C
S
N N N N N N N
N N N N N N N
S
S S SColumbia Blood Agar Base (AM1029/AM5029)
Cam
pylo
bact
er
Sel
ectiv
e Is
olat
ion
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits451
Products for Medical and
Research Institutes
APPLICATIONS
PRODUCTS
Yers
inia
ent
eroc
oliti
ca
Yeas
t & M
ould
s
Vib
rio
Stre
ptoc
occu
s
Sta
phyl
ococ
cus
Shi
gella
Sal
mon
ella
Pro
teus
Pse
udom
onas
Nei
sser
ia
Myc
opla
sma
Myc
obac
teria
List
eria
Legi
onel
la
Kle
bsie
lla
Hae
mop
hilis
E. c
oli
Cor
yneb
acte
rium
Clo
strid
ia
Bru
cella
Bor
dete
lla
Bac
tero
ides
0C - Cultivation, E - Enrichment, I - Identification, N - Non-Selective and S - Selective Isolation. * - Store between 2-8 C.
Cam
pylo
bact
er
Sel
ectiv
e Is
olat
ion
Brucella Selective Supplement* (AS006)
Campylobacter Selective Supplement with Hemin (Karmali) Modified* (AS007)
Staph-Strepto Supplement* (AS025)
Strepto Supplement* (AS026)
Cooked Meat Medium (AM1030/AM5030)
Deoxycholate Agar (AM10303/AM50303)
Deoxycholate Citrate Agar (AM1031/AM5031)
Differential Reinforced Clostridial Broth (AM1038/AM5038)
EC Broth (AM1039/AM5039)
EMB Agar (AM10391/AM50391)
EMB Agar, Levine (AM1040/AM5040)
EM Broth (AM10401/AM50401)
Endo Agar (AM1041/AM5041)
Fluid Lactose Medium (AM1042/AM5042)
Fluid Sabouraud Medium (AM1043/AM5043)
Fluid Selenite Cystine Medium (AM1044/AM5044)
Fluid Thioglycollate Medium (AM1045/AM5045)
G.C Agar Base (AM1046/AM5046)
G.C Supplement* (AS012)
Haemoglobin Solution* (AS014)
Hektoen Enteric Agar (AM104821/AM504821)
Kligler Iron Agar (AM1050/AM5050)
Lauryl Tryptose Broth (AM1053/AM5053)
Legionella Agar Base (AM1054/AM5054)
Legionella Growth Supplement* (AS016)
E/C
S S
S S
C
S
C/S C/S
N N N
C/S C/S
N N N
E E E
E
E E
C
C/S C
S S
I I I I I
E
S
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits 452
Products for Medical and
Research Institutes
APPLICATIONS
PRODUCTS
Yers
inia
ent
eroc
oliti
ca
Yeas
t & M
ould
s
Vib
rio
Stre
ptoc
occu
s
Sta
phyl
ococ
cus
Shi
gella
Sal
mon
ella
Pro
teus
Pse
udom
onas
Nei
sser
ia
Myc
opla
sma
Myc
obac
teria
List
eria
Legi
onel
la
Kle
bsie
lla
Hae
mop
hilis
E. c
oli
Cor
yneb
acte
rium
Clo
strid
ia
Bru
cella
Bor
dete
lla
Bac
tero
ides
0C - Cultivation, E - Enrichment, I - Identification, N - Non-Selective and S - Selective Isolation. * - Store between 2-8 C.
Cam
pylo
bact
er
Sel
ectiv
e Is
olat
ion
Legionella Selective Supplement* (AS017)
Listeria Identification Agar Base,PALCAM (AM1055/AM5055) S
S
C/N
C/N
N N N N N N
N N N N N
N
I I
C
C
C
S
I
I I I
C/S
C/N
Listeria Selective Supplement* (AS008)
Listeria Oxford Medium Base (AM105512/AM505512)
Loeffler Medium Base (AM1056/AM5056)
Horse Serum* (AS015)
Lowenstein Jensen Medium Base (AM1057/AM5057)
Gruft Mycobacterial Supplement* (AS013)
Mac Conkey Agar with Crystal Violet, NaCl and 0.15% Bile Salts(AM1059/AM5059)
MacConkey Agar without Crystal Violet, NaCl and with 0.5% Sodium Tauracholate (AM1061/AM5061)
MacConkey Broth Purple with Bromocresol Purple (AM1063/AM5063)
Malonate Broth Ewing Modified (AM1065/AM5065)
Malt Agar (AM1066/AM5066)
Malt Extract Agar (AM1067/AM5067)
Malt Extract Broth (AM1068/AM5068)
Mannitol Salt Agar (AM1069/AM5069)
M-FC Agar/Broth Base (AM506923/AM506924)
MR-VP Medium (AM1070/AM5070)
Mycoplasma Agar Base (PPLO Agar) (AM1073/AM5073)
Mycoplasma Enrichment Supplement* (AS019)
Horse Serum* (AS015)
Potato Dextrose Agar (AM1082/AM5082)
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits453
Products for Medical and
Research Institutes
APPLICATIONS
PRODUCTS
Yers
inia
ent
eroc
oliti
ca
Yeas
t & M
ould
s
Vib
rio
Stre
ptoc
occu
s
Sta
phyl
ococ
cus
Shi
gella
Sal
mon
ella
Pro
teus
Pse
udom
onas
Nei
sser
ia
Myc
opla
sma
Myc
obac
teria
List
eria
Legi
onel
la
Kle
bsie
lla
Hae
mop
hilis
E. c
oli
Cor
yneb
acte
rium
Clo
strid
ia
Bru
cella
Bor
dete
lla
Bac
tero
ides
0C - Cultivation, E - Enrichment, I - Identification, N - Non-Selective and S - Selective Isolation. * - Store between 2-8 C.
Cam
pylo
bact
er
Sel
ectiv
e Is
olat
ion
Potato Dextrose Broth (AM1083/AM5083)
Preston Agar Base (AM10831/AM50831)
Pseudomonas Agar Base (AM1084/AM5084)
Cetrinix Supplement* (AS008)
CFC Supplement* (AS009)
Pseudomonas Isolation Agar (AM1084117/AM5084117)
Reinforced Clostridial Agar (AM1085/AM5085)
(AM509312)
(AM11002/AM51002
C/N
S
S
S
C
S
C
C
E
I I I I I
S S
C C C
C C C
S
S S S
S
E E
C C
I I I I
C
I I I I
E
Sabouraud Chloramphenicol Agar (AM1086/AM5086)
Sabouraud Dextrose Agar (AM1087/AM5087)
Sabouraud Dextrose Broth (AM1088/AM5088)
Selenite F Broth (AM1089/AM5089)
Simmons Citrate Agar (AM1090/AM5090)
SS Agar (AM1093/AM5093)
Standard Nutrient Agar (AM509311)
Standard Nutrient Broth
Staphylococcus Agar No. 110 (AM10932/AM50932)
Stuart Transport Medium (AM1094/AM5094)
TCBS Agar (AM1095/AM5095)
Tetrathionate Broth Base, Hajna (AM1096/AM5096)
Todd Hewitt Broth (AM10964/AM50964)
Triple Sugar Iron (TSI) Agar (AM1099/AM5099)
Tryptic Digest Broth (AM1100/AM5100)
Tryptone Glucose Beef Extract Agar (TGB Agar)
Tryptone Phosphate Broth (AM1103/AM5103)
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits 454
Products for Medical and
Research Institutes
APPLICATIONS
PRODUCTS
Yers
inia
ent
eroc
oliti
ca
Yeas
t & M
ould
s
Vib
rio
Stre
ptoc
occu
s
Sta
phyl
ococ
cus
Shi
gella
Sal
mon
ella
Pro
teus
Pse
udom
onas
Nei
sser
ia
Myc
opla
sma
Myc
obac
teria
List
eria
Legi
onel
la
Kle
bsie
lla
Hae
mop
hilis
E. c
oli
Cor
yneb
acte
rium
Clo
strid
ia
Bru
cella
Bor
dete
lla
Bac
tero
ides
0C - Cultivation, E - Enrichment, I - Identification, N - Non-Selective and S - Selective Isolation. * - Store between 2-8 C.
Cam
pylo
bact
er
Sel
ectiv
e Is
olat
ion
Tryptone Water (AM1104/AM5104)
Urea Agar Base, Christensen (AM1105/AM5105)
Urea 40%* (AS028)
Urea Broth Base (AM1106/AM5106)
Urea 40%* (AS028)
Violet Red Bile Agar (AM1107/AM5107)
Vogel Johnson Agar Base (AM1108/AM5108)
Potassium Tellurite 1%* (AS022)
XLD Agar (AM1112/AM5112)
Yeast Malt Agar (AM1114/AM5114)
Yeast Malt Broth (AM1115/AM5115)
Yersinia Selective Agar Base (AM1116/AM5116)
Yersinia Selective Supplement* (AS029)
I
I I I I
I I I I
N
S
S S
C/N
C/N
C/S
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits455
Products for Biochemical Tests
Ure
ase
Test
Met
hyl R
ed T
est
Mal
onat
e U
tiliz
atio
n
Leci
thin
ase
Test
Indo
le P
rodu
ctio
n
Hyd
roge
n S
ulph
ide
Pro
duct
ion
Hae
mol
ysis
Tes
t
Esc
ulin
Hyd
roly
sis
Citr
ate
Util
izat
ion
Car
bon
Util
izat
ion
Med
ia W
ith M
ore
Than
O
ne C
arbo
hydr
ate
Med
ia W
ith O
ne
Car
bohy
drat
e
Med
ia W
ithou
tC
arbo
hydr
ates
APPLICATIONS
PRODUCTS
Carbohydrate Fermentation
Deo
xyrib
onuc
leas
e Te
st
Andrade Peptone Water(AM1001/AM5001)
Baird Parker Agar Base(AM1011/AM5011)
BP Sulpha Supplement* (AS005)
Egg Yolk Emulsion* (AS010)
Egg Yolk Tellurite Emulsion* (AS011)
Potassium Tellurite 3.5%* (AS023)
Bile Esculin Agar (AM1012/AM5012)
Blood Agar Base (AM1014/AM5014)
Brain Heart Infusion Agar (AM1016/AM5016)
Bromothymol Blue Lactose Agar (AM1021/AM5021)
Christensen Citrate Agar(AM1025/AM5025)
Columbia Blood Agar Base (AM1029/AM5029)
Decarboxylase agar Base (AM503023)
DNase Test Agar Base (AM10381)
Heart Infusion Agar (AM10481/AM50481)
Staph-Strepto Supplement* (AS025)
Strepto Supplement* (AS026)
Kligler Iron Agar (AM1050/AM5050)
Malonate Broth, Ewing Modified (AM1065/AM5065)
Motility Test Medium (AM506933)
Motility Test Medium ( Edwards and Ewing) BIS(AM506934)
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Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits 456
Products for Biochemical Tests
Ure
ase
Test
Met
hyl R
ed T
est
Mal
onat
e U
tiliz
atio
n
Leci
thin
ase
Test
Indo
le P
rodu
ctio
n
Hyd
roge
n S
ulph
ide
Pro
duct
ion
Hae
mol
ysis
Tes
t
Esc
ulin
Hyd
roly
sis
Citr
ate
Util
izat
ion
Car
bon
Util
izat
ion
Med
ia W
ith M
ore
Than
O
ne C
arbo
hydr
ate
Med
ia W
ith O
ne
Car
bohy
drat
e
Med
ia W
ithou
tC
arbo
hydr
ates
APPLICATIONS
PRODUCTS
Carbohydrate Fermentation
0* - Store between 2-8 C.
Deo
xyrib
onuc
leas
e Te
st
Simmons Citrate Agar (AM1090/AM5090)
MR-VP Medium (AM1071/AM5071)
Nitrate Broth
Peptone Water (AM1079/AM5079)
Peptone Water with Phenol Red (AM1080/AM5080)
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Phenol Red Dextrose Broth (AM50802)
Phenol Red Lactose Broth (AM50803)
Phenol Red Mannitol Broth (AM50806)
Phenol Red Sucrose Broth (AM50808)
Soyabean Casein Digest Agar (AM1091/AM5091)
Triple Sugar Iron (TSI) Agar (AM1099/AM5099)
Tryptone Water (AM1104/AM5104)
Urea Agar Base, Christensen (AM1105/AM5105)
Urea 40%* (AS028)
Urea Broth Base (AM1106/AM5106)
Urea 40%* (AS028)
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Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits457
APPLICATIONS
Cosmetic Analysis
PRODUCTSAnaerobic Agar (AM1000/AM5000)
Baird Parker Agar Base (AM1011/AM5011)
BP Sulpha Supplement* (AS005)
Egg Yolk Emulsion* (AS010)
Egg Yolk Tellurite Emulsion* (AS011)
Potassium Tellurite 3.5%* (AS023)
Brilliant Green Bile Broth 2%(AM1020/AM5020)
Bromothymol Blue Lactose Agar (AM1021/5021)
Cetrimide Agar Base (AM1022/AM5022)
Nalidixic Selective Supplement* (AS020)
Cetrimide Broth (AM1023/5023)
CLED Agar with Andrade Indicator (AM1026/AM5026)
CLED Agar with Bromothymol Blue (AM1027/AM5027)
Cooked Meat Medium (AM1030/AM5030)
Czpek Dox Agar (AM10302/AM50302)
Differential Reinforced Clostridial Broth (AM1038/AM5038)
DNase Test Agar Base (AM10381)
EMB Agar, Levine (AM1040/AM5040)
Endo Agar (AM1041/AM5041)
Fluid Lactose Medium (AM1042/AM5042)
Fluid Thioglycollate Medium (AM1045/AM5045)
Letheen Agar Modified (AM505411)
Letheen Broth Modified (AM50543)
Letheen Broth (AM50542)
Lysine Medium Base (AM10577/AM50577)
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Yeas
t & M
ould
Cou
nts
Yeas
t & M
ould
s
Ste
rility
Tes
t
Sta
phyl
ococ
ci
Pse
udom
onas
Ent
erob
acte
riace
ae
Bac
illus
Bac
teria
l Cou
nts
Ana
erob
es (i
nclu
ding
C
lost
ridiu
m)
Col
iform
s
Lact
obac
illi
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits 458
MacConkey Agar with Crystal Violet, NaCl and 0.15% Bile Salts (AM1059/AM5059)
MacConkey Agar without Crystal Violet and with 0.15% Bile Salts(AM1060/AM5060)
Malonate Broth, Ewing Modified (AM1065/AM5065)
Malt Agar (AM1066/AM5066)
Malt Extract Agar (AM1067/AM5067)
Sabouraud Dextrose Agar (AM1087/AM5087)
Sabouraud Dextrose Broth (AM1088/AM5088)
Soyabean Casein Digest Agar (AM1091/AM5091)
Soyabean Casein Digest Medium (AM1092/AM5092)
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Malt Extract Broth (AM1068/AM5068)
Mannitol Salt Agar (AM1069/AM5069)
MR-VP Medium (AM1070/AM5070)
Nutrient Agar (AM1074/AM5074)
Nutrient Broth (AM1077/AM5077)
Plate Count Agar (AM1081/AM5081)
Potato Dextrose Agar (AM1082/AM5082)
Pseudomonas Agar Base (AM1084/AM5084)
Cetrinix Supplement* (AS008)
CFC Supplement* (AS009)
Pseudomonas Agar (For Fluorescein) (AM108411/AM508411)
Pseudomonas Agar (For Pyocyanin) (AM108414/AM508414)
Pseudomonas Isolation Agar Base (AM108417/AM508417)
Reinforced Clostridial Agar (AM1085/AM5085)
APPLICATIONS
Cosmetic Analysis
Yeas
t & M
ould
Cou
nts
Yeas
t & M
ould
s
Ste
rility
Tes
t
Sta
phyl
ococ
ci
Pse
udom
onas
Ent
erob
acte
riace
ae
Bac
illus
Bac
teria
l Cou
nts
Ana
erob
es (i
nclu
ding
C
lost
ridiu
m)
Col
iform
s
Lact
obac
illi
PRODUCTS
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits 459
APPLICATIONS
Cosmetic Analysis
PRODUCTS
Yeas
t & M
ould
Cou
nts
Yeas
t & M
ould
s
Ste
rility
Tes
t
Sta
phyl
ococ
ci
Pse
udom
onas
Ent
erob
acte
riace
ae
Bac
illus
Bac
teria
l Cou
nts
Ana
erob
es (i
nclu
ding
C
lost
ridiu
m)
Standard Nutrient Broth (AM509312)
Staphylococcus Agar No. 110 (AM10932/AM50932)
Staphylococcus Selection Agar (AM50933)
Triple Sugar Iron (TSI) Agar (AM1099/AM5099)
Tryptone Soya Agar with Lecithin and Tween 80 (AM11031/AM51031)
Vogel Johnson Agar Base (AM1108/AM5108)
Potassium Tellurite 1%* (AS022)
Yeast Malt Agar (AM1114/AM5114)
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0* - Store between 2-8 C.
Col
iform
s
Lact
obac
illi
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Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits460
Cetrimide agar Base
Cetrimide Broth
Czpek Dox Agar
Letheen Broth
Letheen Agar Modified
Letheen Broth Modified
Lysine Medium Base
Plate Count Agar`
Pseudomonas Agar (For Pyocyanin)
Pseudomonas Agar (For Fluorescein)
Pseudomonas Isolation Agar Base
Sabouraud Dextrose Agar
Sabouraud Dextrose Broth
Standard Nutrient Broth
Staphylococcus Selection Agar
Staphylococcus Agar No. 110
Malt Extract Broth
Tryptone Soya Agar with Lecithin and Tween 80
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APPLICATIONS
Product for Environmental and sanitary testing
PRODUCTS
Yeas
t & M
ould
s
Ste
rility
Tes
t
Sta
phyl
ococ
ci
Pse
udom
onas
Ent
erob
acte
riace
ae
Bac
illus
Col
iform
s
Stre
ptoc
occu
s
Lact
obac
illi
Pic
hia
ferm
enta
ns
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Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits461
APPLICATIONS
Textile, Petroleum and Molecular Biology
PRODUCTS
Col
iform
s
Luria Agar
Luria Broth
]Luria Bertani Agar, Miller
Luria Bertani Broth, Miller
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0* - Store between 2-8 C.
Ready to use... Microxpress
Microxpress Dehydrated Culture Media, Bases, Supplements, Ready to use Media, Indicators & Stains, Test Kits 462